[Histonet] Depalstify

[Reuel Cornelia via Histonet]

Hello histonetters,
Regarding my first email. The thickness of my section is 5 micron.


Reuel

 I have a problem with my MMA plastic section on my slide. I could not remove 
the plastic MMA even if I leave the section slides in Xylene for several days 
with heat incubation at 60 degrees. This is the first time that happened to my 
section for several years of doing the same thing over. I was thinking that it 
was my Xylene lot number so I tried to change it but still does not remove the 
plastic, and then Itried different solvent Acetone, and Ethylene glycol 
Monoethyl ether but still it does not work. Can I ask for help if anyone knows 
what was going on and what would be the best way to remove this plastic from my 
section?

Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and 
perkadox 16- 0.5%. This have been my solution for years and I do not have any 
problem with the removal of plastic section until now. I was thinking that my 
MMA (M55909) different lot number from Sigma Aldrich may have cause this 
because even I tried to use the MMA to dissolve my plastic it does not work.


Thank you for and any opinions or protocols are greatly appreciated.


Reuel Cornelia

TSRH

214-559-7766

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pediatric centers for the treatment of orthopedic conditions, certain 
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[Histonet] Deplastifying MMA

[Reuel Cornelia via Histonet]

Hello histonetters,

I have a problem with my MMA plastic section on my slide. I could not remove 
the plastic MMA even if I leave the section slides in Xylene for several days 
with heat incubation at 60 degrees. This is the first time that happened to my 
section for several years of doing the same thing over. I was thinking that it 
was my Xylene lot number so I tried to change it but still does not remove the 
plastic, and then Itried different solvent Acetone, and Ethylene glycol 
Monoethyl ether but still it does not work. Can I ask for help if anyone knows 
what was going on and what would be the best way to remove this plastic from my 
section?

Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and 
perkadox 16- 0.5%. This have been my solution for years and I do not have any 
problem with the removal of plastic section until now. I was thinking that my 
MMA (M55909) different lot number from Sigma Aldrich may have cause this 
because even I tried to use the MMA to dissolve my plastic it does not work.


Thank you for and any opinions or protocols are greatly appreciated.


Reuel Cornelia

TSRH

214-559-7766

--
Texas Scottish Rite Hospital for Children is one of the nation's leading
pediatric centers for the treatment of orthopedic conditions, certain 
related neurological disorders and learning disorders, such as dyslexia. 
This email transmission and/or its attachments may contain confidential 
health information, intended only for the use of the individual or entity
named above.
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[Histonet] Job Opening in Athens, GA

[Jennifer Kempf via Histonet]

Hi...Is it OK to post a position opening?  If so, can you please include this 
in the next histonet email?  Thanks so much!!

 Histology/Grossing technician position open at the University of Georgia, 
College of Vet Med.  This position is primarily for the Grossing Lab, but will 
have regular rotations through the histology side as well.  Posting available 
here:  https://www.ugajobsearch.com/postings/17821.

The University of Georgia is an Equal Opportunity/Affirmative Action employer. 
All qualified applicants will receive consideration for employment without 
regard to race, color, religion, sex, national origin, disability, gender 
identity, sexual orientation or protected veteran status.

Jennifer M Kempf
Histology Laboratory Manager
Department of Pathology
College of Veterinary Medicine
University of Georgia
501 D.W. Brooks Drive
Athens, Georgia  30602
Ph:  706-542-2218
Fax:  706-542-5828
jkemp...@uga.edu



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Re: [Histonet] MMR Negative controls

[Allan Wang via Histonet]

 Hi,

We (at US Biomax) recently screened our colon TMAs for loss of expression
for MLH1, MSH2, MSH6, PMS2 using Ventana's antibodies.

If you need around 10 cases of negative tissue:
CO20813a, 208 cases of colon, contains loss of:
PMS2: 35
MLH1: 18
MSH6: 14
MSH2: 8

This TMA is not listed on our website but is similar in design to CO20813.
We have additional results on other 208 case TMAs too. Feel free to contact
me directly for more details.

Allan

Allan Wang
Lab Manager
US Biomax, Inc.

On Mon, Feb 12, 2018 at 10:37 AM, Lindrud, Scott via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Good Morning HistoNet,
>
> Would anyone be willing to share what they use for control tissue for MMR
> testing?  Finding negative expressing tumor is becoming more difficult for
> us to find and we're wondering if there is an alternate negative tissue
> that would be acceptable.
>
> NordiQC recommends using Tonsil as a positive tissue control and Colon
> Adenocarcinoma that has a loss of expression as a negative tissue control
> for all four antibodies (MLH1, MSH2, MSH6, PMS2).  Stromal cells in the
> negative tissue control should have positive nuclear staining and serve as
> a positive internal control.  NordiQC doesn't have any recommendations for
> alternative tissue to use as a negative control.
>
> Anyone doing anything differently?
>
> Thanks,
>
> Scott
>
> Scott A. Lindrud, MLS(ASCP)CM CTCM
> Histopathology Technical Specialist/Cytotechnologist
> Rice Memorial Hospital
> 301 Becker Ave SW
> Willmar, MN 56201
> WP(320)231-4406
> Fax(320)231-4861
> scott.lind...@rice.willmar.mn.us
>
>
> 
>
> "This e-mail transmission is for the sole use of the intended recipient(s)
> and may contain confidential and privileged information. Any unauthorized
> review, use, disclosure, or distribution violates confidentiality and
> privacy laws and is prohibited. If you are not the intended recipient,
> please notify the sender immediately and delete all copies of the message.
> Thank you."
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] RELIA Special Job Alert Exciting Opportunities Coast to Coast Managers, Supervisors and a little something different! 2-12-2018

[Pam Barker via Histonet]

Hello Histonetters,

I hope you are having a wonderful day.  I am reaching out to you in hopes
that you or someone you know may be interested in a new leadership position.

I have several exciting opportunities for experienced Managers and
Supervisors in hospital and private lab environments in several locations
nationwide.  

These are some of the premier employers in the United States.  The positions
are of course full time and permanent and offer attractive compensation
great benefits and relocation assistance. 
Here is a list of our current leadership opportunities:
Histology Manager - San Francisco Bay Area

Histology Manager - Tampa, FL

Product Manager - San Francisco Bay Area

Histology Supervisor - Birmingham, AL

Field Applications Specialist - San Diego or San Francisco

Team Lead Histology - Birmingham, AL

Our phones are ringing off the hook and we have several confidential
management searched getting ready to start so if you are interested in a new
management opportunity but don't see what you are looking for please feel
free to drop me a line and let me know what to be on the lookout for, for
you!


If you would like more information or know of someone else who might be
interested, please contact me at rel...@earthlink.net or 866-607-3542.
I am available to discuss the opportunity at your convenience including
after hours. Thanks-Pam

 

RELIA Offers A $500 Referral Bonus

For Anyone You Refer And We Place!!

 

There are a lot of recruiters out there right now trying to work with histo
techs and I appreciate your support and respect your needs.  Remember I
offer over 25 years of experience as a recruiter and for over 10 years I
have dedicated my practice solely to placing histology professionals like
you.  

 

 

Thanks-Pam

 

Right Place, Right Time, Right Move with RELIA!

 

Thank You!
 Pam M. Barker

 

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 

 

 

 

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[Histonet] MMR Negative controls

[Lindrud, Scott via Histonet]

Good Morning HistoNet,

Would anyone be willing to share what they use for control tissue for MMR 
testing?  Finding negative expressing tumor is becoming more difficult for us 
to find and we're wondering if there is an alternate negative tissue that would 
be acceptable.

NordiQC recommends using Tonsil as a positive tissue control and Colon 
Adenocarcinoma that has a loss of expression as a negative tissue control for 
all four antibodies (MLH1, MSH2, MSH6, PMS2).  Stromal cells in the negative 
tissue control should have positive nuclear staining and serve as a positive 
internal control.  NordiQC doesn't have any recommendations for alternative 
tissue to use as a negative control.

Anyone doing anything differently?

Thanks,

Scott

Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201
WP(320)231-4406
Fax(320)231-4861
scott.lind...@rice.willmar.mn.us




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may contain confidential and privileged information. Any unauthorized review, 
use, disclosure, or distribution violates confidentiality and privacy laws and 
is prohibited. If you are not the intended recipient, please notify the sender 
immediately and delete all copies of the message. Thank you."
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