Re: [Histonet] detection of ganglion cells and nerve fibers

[Terri Braud via Histonet]

In the past, I've used a rapid acetylcholinesterase on frozen material, but we 
never used Osmium to enhance staining.  I've also used IHC on FFPE sections 
using s-100 and peripherin.  Works great, but it's been awhile, so I don't know 
if there is something better/easier out there now.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

--

Message: 3
Date: Mon, 13 Aug 2018 19:11:34 +
From: "Hobbs, Carl" 
When I was doing them biopsies (  pre-operative) to establish status, we used a 
std histochemical method on frozen sections of biopsy material ( visualising 
acetylcholinesterase activity).
It used Osmium to enhance /darken positivity so, NOT recommended these days.
I recall somebody leaving the lid off the Osmium ( stored at 4C)?..the fridge 
plastic lining was BLACK the next day!
So, any antibody that identifies nerve fibres and ganglion cells should be 
equally effective, using IHC/IF?
Sure, interpretation is critical ( identifying presence/absence of ganglion 
cells and "significant" increase in lamina propria of nerve fibres as 
compensatory mechanism)
There must be an internationally recognised/accepted protocol?
Which technique does Great Ormond Street Hospital/St Thomas' Hospital  use, 
these days?
Curious-illy
Carl
 Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge? 
Kings College London
London
SE1 1UL
? 
020 7848 6813




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Expired SurePath vials

[Charles Riley via Histonet]

Does anyone know how to dispose of expired SurePath vials in Delaware?

Or is anyone able to use expired SurePath vials for research testing or any
other purpose?


Thanks in advance for any replies!!

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RELIA Special Job Alert!!! Exciting Opportunities in NC, TN, GA, AL and FL

[Pam Barker via Histonet]

Hi Histonetters,

How are you? I hope this is the start to a great week for you!

I have several exciting opportunities to share and if you aren't interested
maybe you know someone who might be!

 

I have been engaged on searches by some of my best clients located in North
Carolina, Tennessee, Alabama, Georgia and Florida!

 

My clients offer excellent compensation, benefits and in most cases
relocation assistance or sign on bonuses.

Here's what they are looking for:

.ASCP certified or eligible

.Entry level or experienced

.Opportunity for Advancement

 

The help I need from you Histonetters is do you know anyone that might be
interested in hearing about any of these opportunities?  If so could you
please forward my e-mail to them or pass their contact information to me?
*remember if I place someone you refer to me you will earn a referral bonus!

 

If you are interested in any of these positions please contact me ASAP on my
cell/text 407-353-5070 or toll free at 866-607-3542 

or via email at rel...@earthlink.net

 

If you are interested in positions in other areas of the U.S. please contact
me as well. I have clients nationwide.  I will keep your resume confidential
and I won't release it to anyone without your permission.

 

Thanks-Pam

 

Right Place, Right Time, Right Move with RELIA!

 

Thank You!
 Pam M. Barker

 

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 

https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 

 

 

 

 

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet