[Histonet] cadaver tissue processing for histology

[Preiszner, Johanna via Histonet]

Hi,

I got some cadaver tissue floating in "LTP embalming fluid w/ phenol".

Can I put those straight into the processor? Or has to be rinsed/soaked before? 
If so what should I use?

My Internet searches did not bring up anything useful.

Thank you!

Hanna Preiszner

ETSU/QCOM Dept of Pathology
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Re: [Histonet] special Stains under a hood

[Terri Braud via Histonet]

I have never (3 different places, 30+ years) heard of requiring special stains 
to be performed under a hood.  Certainly, there are a few reagents that might 
need benefit from a hood (concentrated Ammonium Hydroxide) but generally, they 
are performed on an open counter next to a sink.  If you are getting pushback, 
check to see if Micro is doing their gram stain under a hood? 

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal


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[Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?

[Cheryl via Histonet]

Help?   we have a student with one of the online schools and we're doing 
specials like it is 1972.  She has to do a Al Blue 2.5 w Hyaluronidase 
digestion.   We haven't done this since the development of clinical IHC two 
dozen years ago -
Is there any other way to acheive this outcome on a colon slide or do I have to 
order the reagent?
Even your best guess is welcome as we didn't get ahead enough to order the 
reagent and she'll end up submitting a null response (and it will be largely my 
fault :(  ) Cheryl Kerry, HT(ASCP) 
ADG Houston Path

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[Histonet] Special stains and hoods

[Cassie P. Davis via Histonet]

25+ years in Histology, half the places I worked had vents on the wall near the 
countertop where we did specials, otherwise it was on the counterclosest to the 
sink. The only times we were time doing anything under a hood was for frozen 
section staining because of the fresh tissue, mixing sulfer chemicals for 
muscle enzyme studies, diluting acids and the bouins when we microwaved it.  As 
long as there is a sink under the hood too, I wouldn't mind, though.

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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Re: [Histonet] Special stains and hoods

[Morken, Timothy via Histonet]

I guess the question is, who told you this and what is their reference?

Most special stains don't require a hood. Only those that have volatile 
chemicals or odors that may be offensive (ie, ammonium sulfide). We have some 
areas we do specials without a hood. Our main special stain areas have a 
"comfort" hood that is not a real chemical hood but more like a hood in an oven 
hood in a commercial kitchen.  They are not certified or checked in any way and 
we do not claim they are safety hoods.



Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, August 21, 2018 7:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains and hoods

I would like to know how many people perform (manual) routine special stains 
under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - 
but we do them on the counter, not under a hood.  We have been told the 
staining needs to be done under a hood, but I have never done that in my entire 
career.
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Re: [Histonet] Special stains and hoods

[Logan, Shannon via Histonet]

That is certainly an odd request…
I have never performed any special stains under a hood
any place I have ever worked.
Always done on a counter top near a sink…
Is someone in your department extremely sensitive to odors? Or have asthma?
We have a tech who experiences attacks if there are certain chemicals in the 
air however,
she has never complained about the special stains odors.


Shannon H. Logan  B.S., HTL (ASCP)
Pathology Department

Bellin Health Memorial Hospital
744 South Webster Avenue
Green Bay, WI 54305-3400
920-433-3653  X3727



From: Laurie Colbert via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 21, 2018 9:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stains and hoods


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I would like to know how many people perform (manual) routine special stains 
under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - 
but we do them on the counter, not under a hood.  We have been told the 
staining needs to be done under a hood, but I have never done that in my entire 
career.
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[Histonet] MSH2 and MSH6 Loss of Expression

[Knutson, Deanne via Histonet]

Fellow Histonet Members -

We are in the process of moving our IHC bench from the Leica BOND to the ROCHE 
ULTRA and are in the mist of antibody validations.
We are having great difficulty in finding negatives to validate for MSH2 and 
MSH6.
Would anyone have any loss of expression MSH2 and MSH6 appropriate validation 
specimens or cut slides they are willing to share?

Deanne Knutson
Supervisor
Anatomic Pathology

900 East Broadway Avenue, Bismarck, ND 58501-4520
P 701-530-6730  I  F 701-530-6735
dknut...@primecare.org
st.alexius.org
 [X]
 "Let All Be Received as Christ."



  
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