[Histonet] LDT question
Hi All, I have a validation question - we are looking into buying an RUO rated IHC stainer for a future multiplexing project. Roche's Discovery Ultra to be specific. I have a pathologist that is convinced that we can run our clinical antibodies on it as an LDT and still bill for the work. The material I have read up to this point regarding that instrument explicitly states that no slides can be run for diagnostic purposes. The instrument has software that allows you to use it to mimic the IVD instruments' software capabilities so you can validate a protocol on it, then move it right over to the Benchmark so I can see why the idea that it can run our IVD items seems plausable. However what I need to know is which is correct? I do not want to do something that I am uncomfortable doing without checking with others in my field first. Thanks! -- Erin McCarthy, HT (ASCP) Histotechnician Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws. It is intended solely for the use of Tempus Labs and the recipient(s) named above. Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality. If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited. * If you have received this transmission in error, please notify us immediately at **(855)-442-8305** and permanently delete this email and any attachments*. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
You are right! Thank you for correcting me. Surely sialomucins will be positive for PAS and H.acid will be negative? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From: Mac Donald, Jennifer Sent: 23 August 2018 19:52:52 To: Hobbs, Carl Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0. -Original Message- From: Hobbs, Carl via Histonet Sent: Thursday, August 23, 2018 11:32 AM To: histonet Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7Cc3bfd2048e1b4508a26b08d60929a33c%7C8370cf1416f34c16b83c724071654356%7C0sdata=CTVwv5AklHrbGKIoml5W4NVoYJ7Skdfro9F%2FXE0uuWM%3Dreserved=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0. -Original Message- From: Hobbs, Carl via Histonet Sent: Thursday, August 23, 2018 11:32 AM To: histonet Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cadaver tissue processing for histology
Rinse in dist water ( or tap water) x3 over 30 mins No big deal to do this but nice to get rid of extra smells in your processing reagents ( depending on where you start processing: I start in 90% alcoholsure, I ask for recd specimens to be rinsed in water then transferred to 70% alcohol before I will process them) Hopefully, you don't want to do IHC as inclusion of Glut. is counterproductive. Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives?
Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] LTP fluid with Phenol
Here is the approximate chemical make-up of LTP with Phenol. (LTP stands for Long Term Preservation) Ethanol 48.% Formaldehyde 20.% Ethylene Glycol 4.% Phenol 11.% Glutaraldehyde 4.% Due to the presence of Ethylene Glycol, I would let the tissue set in a separate container of 10% Neutral Buffered Formalin for 24 hours before processing as usual. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 4. cadaver tissue processing for histology (Preiszner, Johanna) Message: 4 Date: Wed, 22 Aug 2018 18:59:44 + From: "Preiszner, Johanna" Subject: [Histonet] cadaver tissue processing for histology Hi, I got some cadaver tissue floating in "LTP embalming fluid w/ phenol". Can I put those straight into the processor? Or has to be rinsed/soaked before? If so what should I use? My Internet searches did not bring up anything useful. Thank you! Hanna Preiszner ETSU/QCOM Dept of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Windsurg Reports
Afternoon, Looking to see if anyone has some tipsheets on how to pull embedding logs or accession logs from winsurg. I am by no means familiar with the system so if you could send me step by step instructions I would really appreciate it!! Thanks! Jessica Vacca HCA Epic Anatomic Pathology Lead ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
