[Histonet] Platelet staining on FFPE mouse tissues
Hi everyone, Does anyone has experience performing IHC staining for platelets on mouse FFPE? I've been trying for a while with different primary antibodies unsuccessfully. I found two that work in cryosections, but not a single hint of positivity on FFPE tissues. The samples have been prepared as usual (NBF fixation, graded EtOH, Xylene, wax...) and thin sections were collected by usual procedures (think they are 5 um). Unfortunately I have limited resources, so my protocol has been manual staining (no autostainer) and I have only microwave or water baths available around to play with Ag retrievals. I also already tried Sodium Citrate and Citrate buffers at pH = 6 and 9. Anyone has a protocol that works and that they would be willing to share? Much appreciated! Kind regards, Ana Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting priv...@baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hope to See You in St. Louis at the NSH SC! And Here are some Exciting New Opportunities!
Hi Histonetters, How are you? As you know I do the majority of my work via email and phone so I rarely get a chance to meet people in person. That is one of the many reasons I enjoy attending the NSH conventions. I wanted to drop you a line and see if you or anyone you work with is planning on attending the NSH convention in St. Louis next week? If so I would love to have the opportunity to say Hi. I will be very easy to find because I am working the registration desk all day Friday, Saturday, Sunday and Monday so please if you are going to be there stop by and say Hi. Also if you haven’t already done so, check out the NSH convention app. It is available at the NSH convention Attendee website. It has some really cool features to enhance your convention experience. If you aren’t attending this year follow the fun on Facebook or on twitter we will be posting with the hashtag #nshsc one of my volunteer responsibilities this year is social networking so I will be posting lots of pics and statuses about all the exciting stuff going on so stay tuned!! If you are interested in a new opportunity I have some exciting H • Virginia (Senior Tech) • North Carolina (Management and Tech positions) • SW Florida – (Mohs Tech) • California – (Get off the Bench!) • Chattanooga (IHC) • Missouri – KC – Senior Tech All of these positions are full time and permanent and most of my clients offer relocation assistance and or a sign on bonus. For more info please contact me at rel...@earthlink.net or on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. Hope to see you in St. Louis!! ☺ Have a great day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
