Re: [Histonet] AFB STAIN
We do, It works well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, 4 October 2018 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Annual Competency
Hi, I am in the process of updating the competencies for histotechs and lab Assistant in our lab. I will appreciate it, if you guys could share. Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AFB STAIN
Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O
Hi, I have seen this too. I mitigate the problem by making it up in 50 ml increments and staining them flat. I draw from around the middle of the Falcon tube I make it up in. It tends to precipitate so you could filter it. Don't bother re-using the reagent. It's cheap & easy to make up. I usually only see the precipitate with old solutions. Just make what you use within a few weeks. Best 'o luck, Amos > > Message: 2 > Date: Wed, 3 Oct 2018 15:26:25 + > From: Reuel Cornelia > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] Oil Red O > Message-ID: > < > sn1pr17mb0543f87dbc5e39aa8875c9f1e7...@sn1pr17mb0543.namprd17.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > I was wondering if you could help me know why our Oil Red O have some > black snowflakes on our fat tissue hours after staining or after 24 hours > more snowlflakes precipitation occurs. The staining was reference was from > Lillie RD, Ashburn. Please note that our staining works well between two to > three hours. Is there a reason fro this precipitation? > > > Reuel > > TSRH > > Dallas, TX > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil Red O
Hi Reuel, In my experience this is the ORO crystallizing out on the section. I would first filter your staining solution, then keep your staining times to as short as you can. Also changing between 37 and room temperatures may be useful too. mills ᐧ On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I was wondering if you could help me know why our Oil Red O have some > black snowflakes on our fat tissue hours after staining or after 24 hours > more snowlflakes precipitation occurs. The staining was reference was from > Lillie RD, Ashburn. Please note that our staining works well between two to > three hours. Is there a reason fro this precipitation? > > > Reuel > > TSRH > > Dallas, TX > > -- > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the treatment of orthopedic conditions, certain > related neurological disorders and learning disorders, such as dyslexia. > This email transmission and/or its attachments may contain confidential > health information, intended only for the use of the individual or entity > named above. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O
I was wondering if you could help me know why our Oil Red O have some black snowflakes on our fat tissue hours after staining or after 24 hours more snowlflakes precipitation occurs. The staining was reference was from Lillie RD, Ashburn. Please note that our staining works well between two to three hours. Is there a reason fro this precipitation? Reuel TSRH Dallas, TX -- Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide folders with space for blocks too
I want to order some folders that have space for blocks to go with the slides. I swear this exists - I remember very clearly bringing it up as a suggestion at my last job and the pathologist there shutting it down instantly (he didn't want to be responsible for checking the block against the slide himself). The only ones I can find googling now are these: http://sourcemp.com/consulting-folder-for-slides-and-blocks.aspx But I am certain I saw them before with room for 20, and they were plastic if I remember right. Anyone else remember these things, or have I lost my mind? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
