[Histonet] counter stains for Sudan Black B

[Tyrone Genade via Histonet]

Hello,

I need a counterstain for use together with Sudan Black B. I am using this
method: https://www.ncbi.nlm.nih.gov/pubmed/27812872 which uses Nuclear
Fast Red. I don't have nuclear fast red... In an updated protocol by these
authors they use hematoxylin but in a previous trial I found it was too
dark and make visualizing the Sudan Black signal too difficult. I'm using
Sigma's Mayer's Hematoxylin solution. It is past its sell-by date and just
won't blue nicely any more..

I have available Methyl Green, Neutral Red and  Safranin  O. The former
washes out in aqueous/glycerol mounting medium (required for the protocol).
How well does Neutral Red and Safranin work with an aqueous mounting medium?

Thanks
-- 
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

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[Histonet] Gout specimens

[Anne Murvosh via Histonet]

I haven't done a gout specimen in years, I remember the processing part, not 
remembering the Staining part. Could someone please send me your gout protocol? 
Thanks Anne Murvosh HT

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[Histonet] Freezing artefact

[Finlay Finlay via Histonet]

Good Afternoon all,

I have a couple of cases where my post mortem samples have been accidentally 
frozen in Neutral Buffered Formalin. Naturally this has led to pretty striking 
artefact in the tissue. I was wondering if anyone who perhaps has more 
experience of dealing with frozen tissue has any tips on minimising this 
artefact? I still have some fixed tissue but am unable to resample from the 
unfixed tissue. Thanks in advance.

Finlay Finlay

Senior Histology Technician
Forensic Medicine and Science
School of Medicine
College of Medical, Veterinary and Life Sciences
University of Glasgow
Joseph Black building

Direct Line: +44 (0) 141 3303443
E-mail: finlay.fin...@glasgow.ac.uk

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