[Histonet] possible answer

2019-02-08 Thread Cassie P. Davis via Histonet 
Reguarding:
Hello fellow Histonetters
I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.
Thanks for the assistance, I definitely appreciate it very much.

Best wishes,

[image001]  Debbie Siena, HT(ASCP)QIHC
Empowering Anatomic Pathology
Technical Support Manager, StatLab
2090 Commerce| McKinney, TX 75069
t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369
dsi...@statlab.com|www.statlab.com>


What you are seeing may be caused by the surgeon waiting before placing the 
specimen into the formalin. There are several newer studies indicating ischemic 
time adversely affecting IHC and since the outside of the specimen seems to be 
showing this, it would seem to fall in-line with the theory.

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



Confidentiality Notice:
This e-mail, including any attachments is the property of Trinity Health and is 
intended for the sole use of the intended recipient(s). It may contain 
information that is privileged and confidential.  Any unauthorized review, use, 
disclosure, or distribution is prohibited. If you are not the intended 
recipient, please delete this message, and reply to the sender regarding the 
error in a separate email.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Derm IHC question

2019-02-08 Thread Cartun, Richard via Histonet 
Doesn't sound like a fixation issue to me.  Could the tissue be drying out 
before it's placed in formalin?  Also, are these specimens inked for assessment 
of margins?  I've seen ink interfere with immunoreactivity.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Debra Siena via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 11:51 AM
To: 'histonet'
Subject: [Histonet] Derm IHC question

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

Hello fellow Histonetters

I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.

Thanks for the assistance, I definitely appreciate it very much.



Best wishes,



[image001]  Debbie Siena, HT(ASCP)QIHC

Empowering Anatomic Pathology

Technical Support Manager, StatLab

2090 Commerce| McKinney, TX 75069

t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369

dsi...@statlab.com|https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M=y7Dkipiv1JdLkqba8XolhtugffjOReKOTWuW0mZaJJI=



StatLab is an ISO 13485 Certified Company



___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M=9GfWQBzHH2U1LIbnBXO5wvUeCTlJulcFAkXN5aWrbCw=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ER/PR question

2019-02-08 Thread Cartun, Richard via Histonet 
If the tumor is ER/PR negative the first thing I do is to look for an internal 
positive control (immunoreactive benign breast epithelium).  In my experience, 
the majority of these cases have internal positive controls to validate the 
negative ER/PR results.  When I don't see internal positive controls, and the 
HER2 is negative, I recommend that the testing be repeated on excisional tumor 
for confirmation.  This is why it's important to submit adjacent benign breast 
tissue along with the tumor tissue when grossing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Greg Dobbin via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 8:52 AM
To: karen.heckf...@dignityhealth.org; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] ER/PR question

CAUTION: This email is from outside HHC. USE CARE when opening attachments or 
links.

Hi Karen,

As mentioned by others "decay" is not likely going to be an issue. More

concerning for you could be not knowing how those tissues were handled

prior to processing 10 years ago.



Presumably, you now track cold ischemic times and have standardized your

fixation protocols for breast tissues and you have validated your IHC

procedures with these current parameters. If your lab was like many others

10 years ago, these parameters were probably not maintained so rigorously.

So interpretation of a negative result may be problematic.



Having said that, there is the internal control for both ER and PR. It

seems to me that if the internal control is staining adequately, then

perhaps interpretation will not be an issue. I am curious, does anyone have

a different take on relying the internal control as a guide in this

particular situation??

Greg



--

*Greg Dobbin*

1205 Pleasant Grove Rd

RR#2 York,

PE  C0A 1P0





*Everything in moderation...even moderation itself**!*

___

Histonet mailing list

Histonet@lists.utsouthwestern.edu

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE=6t6lv-HaXrWz9L26Ub8EE5c9k76MJqlTDQ1XNA9cb2Q=_Gu4paw-d2iygUvP770iXrMhJFfeQlU9bt3MZLe-NIA=



Reminder: This e-mail and any attachments are subject to the current HHC email 
retention policies. Please save or store appropriately in accordance with 
policy.

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Derm IHC question

2019-02-08 Thread Debra Siena via Histonet 
Hello fellow Histonetters
I would like to ask you a question about IHC staining and derm cases.  I am 
seeing a peculiar issue going on, where the melanocytes in the middle of the 
tissues are staining pretty well but when you get to the ends of the tissues 
either shaves or ellipses, they are not staining. This is sporadic, not every 
case and there is no consensus as to a common thread between the cases.I 
feel that this may be a fixation issue but was just wondering if anyone had 
ever seen the same phenomena and would be willing to share the theory or even 
better what was the remedy behind this issue.  The fixative is 10% Neutral 
Buffered Formaliln  and the cells in question that are "dropping out" which is 
what the pathologist is describing are melanocytes, especially with Sox-10 and 
Mart 1 antibodies.
Thanks for the assistance, I definitely appreciate it very much.

Best wishes,

[image001]  Debbie Siena, HT(ASCP)QIHC
Empowering Anatomic Pathology
Technical Support Manager, StatLab
2090 Commerce| McKinney, TX 75069
t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369
dsi...@statlab.com|www.statlab.com

StatLab is an ISO 13485 Certified Company

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] ER/PR question

2019-02-08 Thread Greg Dobbin via Histonet 
Hi Karen,
As mentioned by others "decay" is not likely going to be an issue. More
concerning for you could be not knowing how those tissues were handled
prior to processing 10 years ago.

Presumably, you now track cold ischemic times and have standardized your
fixation protocols for breast tissues and you have validated your IHC
procedures with these current parameters. If your lab was like many others
10 years ago, these parameters were probably not maintained so rigorously.
So interpretation of a negative result may be problematic.

Having said that, there is the internal control for both ER and PR. It
seems to me that if the internal control is staining adequately, then
perhaps interpretation will not be an issue. I am curious, does anyone have
a different take on relying the internal control as a guide in this
particular situation??
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet