[Histonet] Deep Water Bath with Lighting

2019-12-18 Thread Cooper, Brian via Histonet
I really like the TFB 35 flotation baths from Medite. They make a shallow and a 
deeper dish version; you'll want to go with the deeper dish. I'm not in front 
of one right now, but I suspect they're about 3" give or take.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles

Sent from my cell phone, so please excuse typos


On Dec 18, 2019 3:45 PM, Sandra Cheasty via Histonet 
 wrote:
Ho-ho-ho all,
I'm looking for a deep water bath, (3" minimum), that has an 
interior light source. Most of them seem to be only 2" tall, or have a slanted 
front control panel that none of us in the lab want. Does anyone know of a 
brand of flotation water baths that are 3" deep and have interior lighting?
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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[Histonet] Deep Water Bath with Lighting

2019-12-18 Thread Sandra Cheasty via Histonet
Ho-ho-ho all,
I'm looking for a deep water bath, (3" minimum), that has an 
interior light source. Most of them seem to be only 2" tall, or have a slanted 
front control panel that none of us in the lab want. Does anyone know of a 
brand of flotation water baths that are 3" deep and have interior lighting?
Cheers!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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[Histonet] Large Format Cover Glass

2019-12-18 Thread Rebecca E. Ashley via Histonet
Does anyone know of a good source for cover glass for the large format slides?  
48x60mm specifically.  We do a lot of eyes from large animals and I'm having a 
hard time finding coverslips big enough for a horse eye!
Thanks,
Rebecca

Rebecca Ashley, HTL (ASCP)CM
Laboratory Technician III
Wyoming State Veterinary Laboratory
1174 Snowy Range Rd.
Laramie, WY 82070
307-766-9946

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Re: [Histonet] Delay in embedding?

2019-12-18 Thread Hobbs, Carl via Histonet


I agree with replies/statements 3, 4, 5
Anyone who states differently have not experienced this contingency.



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 

020 7848 6813
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Re: [Histonet] plural fluid prep

2019-12-18 Thread John Garratt via Histonet
The cells have been living quite nicely in a natural culture medium at 37c so 
putting the pleural fluid at 4c will preserve the cells for a number of days. I 
do not think I would want to go past 3 to 4 days, but that is just the limit of 
my experience revisiting old ascitic fluid specimens.
You could use alcohol to preserve the specimen longer but not knowing what your 
experiment is I am loath to offer an opinion on that.

John

Sent from ProtonMail Mobile

On Wed, Dec 18, 2019 at 9:51 AM, Michelle Jamison via Histonet 
 wrote:

> Hi All,
> I work in a research lab where we are using plural fluid to proof a
> method. Recently we have received some, and are unsure how long it will
> be ok in the fridge, or if we should add some preservation fluid to it.
> Can anyone advise please.
> Thank you in advance, and Happy Holidays to all!!
> --
>
> Best of all things to you,
>
> Michelle Jamison
>
> //
>
> Tel : +1.435.752.6011 Ext. 435.227.1474
>
> m.jami...@elitechgroup.com  • www.elitechgroup.com
> 
>
> 
>
> Logo
>
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[Histonet] plural fluid prep

2019-12-18 Thread Michelle Jamison via Histonet

Hi All,
I work in a research lab where we are using plural fluid to proof a 
method. Recently we have received some, and are unsure how long it will 
be ok in the fridge, or if we should add some preservation fluid to it. 
Can anyone advise please.

Thank you in advance, and Happy Holidays to all!!
--

Best of all things to you,

Michelle Jamison

//

Tel : +1.435.752.6011 Ext. 435.227.1474

m.jami...@elitechgroup.com  • www.elitechgroup.com 





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[Histonet] Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy!

2019-12-18 Thread Pam Barker via Histonet
Hi Histonetters,
Happy Holidays!
Here is a gem posted on the histonet in 12/2013 

The 12 days of Christmas for Histotechs !
written by Ashley Troutman

“Hello all,
I hope the holidays are treating everyone well. (Especially in light of us
having the most hazardous job in the country...) Here's some fun for the
season:
The Twelve Days of Histo
TWELVE Medical Directors
ELEVEN logs-a-printing
TEN residents reading
NINE frozen sections
EIGHT administrators
SEVEN stainers beeping
SIX techs complaining
FIVE I...H...Cs  :)
FOUR calling docs
THREE recuts
TWO special stains
And a block with an H and E...
Please feel free to sing along.
Merry Christmas in Histoland!”
Ashley Troutman BS, MBA, HT(ASCP) QIHC

Histonetters,
I have some REALLY Exciting opportunities, 
Here’s a quick list:
Leadership:
MA - Field Applications Specialist – IHC- Boston
IL - Field Applications Specialist – IHC – Chicago
MO - Histology Lab Manager – St. Louis
TN - Histology Supervisor - Nashville
Histotechnicians/Histotechnologists
North Carolina
South Carolina
Wisconsin
Florida
Kansas
New York
California
I have new opportunities coming in all of the time.  Let me know if anything
looks interesting on my list or if there is a specific area you would like
for me to be on the lookout for.  
We can start chatting now about opportunities after the new year!!
The early bird gets the worm!!
I also can help if you are short staffed!
I can be reached asap on my cell/text at 407-353-5070 or email me at
rel...@earthlink.net

Happy Holidays to you and yours

Happy Holidays 

Thanks-Pam

#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 




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Re: [Histonet] Delay in embedding?

2019-12-18 Thread Garrey Faller via Histonet
I’ve done it a handful of times when my only Histotech called in sick.
No effect on quality.

Garrey

Sent from my iPhone

> On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet 
>  wrote:
> 
> I don’t see a problem with it. We all melt and re-embed tissue for all kinds 
> of reasons every day. I think it is much better for the tissue than keeping 
> it warm for an extended period of time. Jen Saunders 
> 
> 
> Sent from Yahoo Mail for iPhone
> 
> 
> On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet 
>  wrote:
> 
> Hi Paula,
> 
> We routinely do this, especially for our fetal autopsy blocks.
> We are then able to process and let them set at room temp until we are able 
> to embed and cut.
> Some cases are more urgent than others so these can be expedited a lot easier 
> since they will only need embedding, sectioning and staining. We also get 
> best usage of our limited processing capabilities.
> It is more efficient for us.
> 
> Morphology, staining and immunohistochemistry is not affected.
> 
> It is better to do this rather than leave them at 64oC plus for extended 
> times prior to embedding since many antigens will be adversely affected.
> 
> Take home point - do not overcook your tissues.
> 
> 
> 
> Regards 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Principal Scientist, the Children’s Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney 
> Tel: 612 9845 3306 
> Fax: 612 9845 3318 
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> -Original Message-
> From: Theresa Dalton via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Wednesday, 18 December 2019 7:34 AM
> To: P Sicurello 
> Cc: HistoNet 
> Subject: Re: [Histonet] Delay in embedding?
> 
> We have done this - only on an emergency basis. 
> 
> Sent from my iPhone
> 
>> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet 
>>  wrote:
>> 
>> Good Morning Listers,
>> 
>> 
>> 
>> How many out there will process tissue and then leave the cassettes at 
>> room temperature and embed it at a later time (hours or the next day)?
>> 
>> 
>> 
>> Please send me your opinions about doing this.  I think it’s a bad 
>> idea, others I speak with disagree.
>> 
>> Sincerely,
>> 
>> Paula Sicurello, HTL (ASCP)CM
>> 
>> Histotechnology Specialist
>> 
>> UC San Diego Health
>> 
>> 9300 Campus Point Drive
>> 
>> La Jolla, CA 92037
>> (P): 858-249-5610
>> 
>> 
>> 
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