[Histonet] Question about gelatin embedding

[Alonso Martínez Canabal via Histonet]

Hello,
  I am here again. I am wondering if someone has good experience
embedding in gelatin-albumin for cryostat or vibratome sectioning.
Specifically we use brain tissue and is common in free floating techniques
non-attached parts of the same section float around and later that
generates all sorts of problems.
   Thank you very much.

-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 194, Issue 12btattoo removal

[Steve McClain via Histonet]

I don not know any method for tattoo pigment removal.  Aside from carbon black, 
most of the red, yellow, blue and green pigments are metal salts and polarize 
brightly.  The inflammatory response may be vigorous, especially w repeat or 
Re-do of a tattoo. In some cases a pseudo-carcinoma or KA may result.

I am puzzled to learn why the request!  Can anyone in the group think of a 
reason why or purpose for the pathologist needs to request removal?Perhaps 
Staining of infectious organism?

Steve
Steve A. McClain, MD
631-361-4000  Cell 631-926-3655

Hello fellow histonetters,

We received a skin sample that has ink from a tattoo - the sample if from
tattooed skin. One of our pathologists would like us to see if we can get
rid of the tattoo ink from the sections before H staining. Does anyone
out there know how to do this?

Thank you,
Donna Emge
Anatomic Pathology Manager
Mercy Hospital and Medical Center, Chicago

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Prepared slides of human skin with tattoos in xs

[Jorge A. Santiago-Blay via Histonet]

Hi:

I teach college human anatomy and physiology and would like to have a slide
or two of human skin with tattoos in cross section. Do you know where may I
get a hold of those? All I use these days is an illustration from the web.
Please, email me directly if you have constructive suggestion.

blaayjo...@gmail.com

Gratefully,

Jorge

Jorge A. Santiago-Blay, PhD
*https://blaypublishers.com *

1. Positive experiences for authors of papers published in *LEB*
http://blaypublishers.com/testimonials/

2. Free examples of papers published in *LEB*:
http://blaypublishers.com/category/previous-issues/.

3. *Guidelines for Authors* and page charges of *LEB*:
http://blaypublishers.com/archives/ *.*

4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/

http://blayjorge.wordpress.com/

http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] 3rd party block and slide storage fees

[Olszewski, Dawn via Histonet]

Hi everyone,

We are a smaller hospital that stores our blocks, slides and files off site 
with a 3rd party storage facility.  Does anyone have any information on fees 
for storage of blocks, slides, records and how much they charge to pick-up and 
deliver when needed and the names of any companies you prefer to use?

Thank you all as always for your help.

Dawn Olszewski, HTL(ASCP)QIHC
Pathology Manager
South Georgia Medical Center
P: (229) 259-4830
E:  dawn.olszew...@sgmc.org



Dawn Olszewski
Pathology Manager - Histotechnologist
Laboratory

[cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png]

South Georgia Medical Center
2501 N. Patterson St.
Valdosta, GA 31602
229-259-4830 (O) | 229-560-6191 (M)
dawn.olszew...@sgmc.org | sgmc.org

[cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png]
  [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] 
   
[cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] 
   
[cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] 







___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FW: New Post: Strategies for Coping With the Shortage of Histotechs

[Pam Barker via Histonet]

Hi Histonetters!

Here is my latest blog post on the NSH - Fixation on Histology site:

"Strategies for Coping with the Shortage of Histotechs.

TGIF.  Have a wonderful weekend!

 

Thanks-Pam

From: Fixation on Histology [mailto:hi...@nsh.org] 
Sent: Friday, January 17, 2020 1:07 PM
To: rel...@earthlink.net
Subject: [SPAM] New Post: Strategies for Coping With the Shortage of
Histotechs

 

 Please click here to access the web/mobile version
 




 fixationonhistology_60470.PNG
 


Check out Fixation on Histology's Newest Post: Strategies for Coping With
the Shortage of Histotechs
 

By: Pam Barker, Relia Solutions

 




 needjob_960971.PNG
 



Has there ever been a time when there wasn't a shortage of histotechs? It
just seems to get more critical every year. I have to be honest I would LOVE
to place techs with all of you but they aren't always out there. I wanted to
write this article to encourage you and give you strategies to cope with the
shortage because I think we can all agree that this shortage is not going
away.





 
 Continue Reading



  _  


 


Interested in Contributing?


NSH is accepting submissions for Fixation on Histology! Share your stories,
advice, or research with others in the histology profession!






 
 Submit Your Post 






 National Society for Histotechnology
 


National Society for Histotechnology 

3545 Ellicott Mills Dr. 

Ellicott City, MD 21043 

  

Phone: 443:535-4060 

Fax: 443-535-4055 

Email: hi...@nsh.org 


Follow Us:
 facebook
 twitter
 linkedin
 linkedin

 

Subscribe to our Blog:
 Fixation on Histology


Unsubscribe
  from these messages. 

 

3545 Ellicott Mills Drive, Ellicott City, MD 21043


   

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis

[Charles Riley via Histonet]

The only way I know of is through lasers

On Fri, Jan 17, 2020 at 12:25 PM John Garratt via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> It is a pigment (ie carbon) and not like a soluble ink, so good luck with
> that.
> You could consult your histotech text book on exogenous pigment removal
> and give the recipe a go. I will certainly be interested in other histonet
> replies.
>
> John
>
>
>
> www.cpqa.ca
>
> ‐‐‐ Original Message ‐‐‐
> On Friday, January 17, 2020 8:16 AM, Donna Emge via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Hello fellow histonetters,
> >
> > We received a skin sample that has ink from a tattoo - the sample if from
> > tattooed skin. One of our pathologists would like us to see if we can get
> > rid of the tattoo ink from the sections before H staining. Does anyone
> > out there know how to do this?
> >
> > Thank you,
> > Donna Emge
> > Anatomic Pathology Manager
> > Mercy Hospital and Medical Center, Chicago
> >
> >
> -
> >
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis

[John Garratt via Histonet]

It is a pigment (ie carbon) and not like a soluble ink, so good luck with that.
You could consult your histotech text book on exogenous pigment removal and 
give the recipe a go. I will certainly be interested in other histonet replies.

John



www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Friday, January 17, 2020 8:16 AM, Donna Emge via Histonet 
 wrote:

> Hello fellow histonetters,
>
> We received a skin sample that has ink from a tattoo - the sample if from
> tattooed skin. One of our pathologists would like us to see if we can get
> rid of the tattoo ink from the sections before H staining. Does anyone
> out there know how to do this?
>
> Thank you,
> Donna Emge
> Anatomic Pathology Manager
> Mercy Hospital and Medical Center, Chicago
>
> -
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Skin Sample with Tattoo - need to remove for diagnosis

[Donna Emge via Histonet]

Hello fellow histonetters,

We received a skin sample that has ink from a tattoo - the sample if from
tattooed skin. One of our pathologists would like us to see if we can get
rid of the tattoo ink from the sections before H staining. Does anyone
out there know how to do this?

Thank you,
Donna Emge
Anatomic Pathology Manager
Mercy Hospital and Medical Center, Chicago

--
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] VERBAGE FOR CUTTING MULTIPLE SPECIMENS ON MICROTOME

[Baldwin, Kathy via Histonet]

Standard protocol for replacing microtome blades anybody have this or can help??

Thanks
S Kathy Baldwin ASCP, SCT
Memorial Hospital and Health Care Center
Pathology and Cytology Manager
Ph 812-996-0210
Fax 812-996-0232


CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information or otherwise protected by law. Any unauthorized review, 
use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all copies of 
the original message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] WVU Medicine

[Hall, Jessica via Histonet]

Good Morning,

We have recently opened a new Histotechnologist position with WVU Medicine!  
This position is eligible for a generous sign-on bonus, full comprehensive 
benefit package, and a competitive salary range.  I invite you to contact me 
with any questions about this exciting opportunity to join the region's largest 
health system!
To submit your application for consideration, click here:  
https://re12.ultipro.com/WES1019WVUH/jobboard/NewCandidateExt.aspx?__JobID=48450

WVU Medicine - Work Here, Live Here, Thrive Here - Click Here to watch video 
about our Health System

/ Jessica Hall,  CIR, SHRM-CP, AASPR, STA
Supervisor, Talent Acquisition
Human Resources
WVU Medicine
Office: 304-598-6689
Cell: 304-709-4783





 Confidentiality Notice: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information. Any unauthorized review, use, disclosure or 
distribution is prohibited. If you are not the intended recipient, please 
contact the sender by reply e-mail and destroy all copies of the original 
message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cell block preparations

[Tony Reilly via Histonet]

Some predictive text issues like lyse and nucleated. 

Sent from my iPhone

> On 17 Jan 2020, at 8:24 am, Tony Henwood (SCHN) via Histonet 
>  wrote:
> 
> Hi Jennifer,
> 
> I have had excellent success with lysing the red blood cells (using  Isotonic 
> Ammonium Chloride) prior to cell block preparation with 
> thromboplastin-plasma. 
> The lysing solution contains EDTA so you will need to add a few drops of 1% 
> calcium chloride. Method as follows:
> 
> Lysis solution
> Ammonium Chloride4.5g
> Potassium carbonate  0.5g
> EDTA 0.0186g
> Distilled water  500mls
> 
> Method:
> 1.Centrifuge bloody fluid.
> 2.Remove supernatant and add equal volume of lysis solution.
> 3.Resuspend and incubate for 5 minutes at 4oC.
> 4.Centrifuge, if blood still remains, then repeat from step 2.
> 5. Rinse in Hanks or RPMI, centrifuge.
> 6.Mix pellet in a few drops of plasma.
> 7. Add thromboplastin and a few drops  of 1% Calcium Chloride, mix gently 
> and allow clot to form.
> 8. Add 10% buffered formalin and fix and process as usual.
> 
> Reference:  
> Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479
> 
> If you donot use the plasma clot method for cell block preparation, then use 
> your preferred method after step 5.
> The lysis solution can also be purchased commercially from several companies 
> (eg Biolegend). It is commonly used for sample preparation for flow 
> cytometry. Check the SDS to make sure it does not contain formaldehyde.
> 
> 
> -Original Message-
> From: Mac Donald, Jennifer via Histonet 
> [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Friday, 17 January 2020 4:53 AM
> To: Charles Riley ; Histo List 
> 
> Subject: Re: [Histonet] Cell block preparations
> 
> Acetic acid would work.
> 
> Get Outlook for iOS 
> From: Charles Riley via Histonet 
> Sent: Thursday, January 16, 2020 8:55:21 AM
> To: Histo List 
> Subject: [Histonet] Cell block preparations
> 
>  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.
> 
> What is the best way to remove excess blood from FNA sample collections 
> before spinning them down into cell blocks?
> 
> --
> 
> Charles Riley BS  HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> This message is intended for the addressee named and may contain confidential 
> information. If you are not the intended recipient, please delete it and 
> notify the sender.
> 
> Views expressed in this message are those of the individual sender, and are 
> not necessarily the views of NSW Health or any of its entities.
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Cell block preparations

[Tony Reilly via Histonet]

Hi Charles

A method my EM scientist used many years ago was quite simplistic and he 
thought it worked well.
You simply centrifuge the specimen,  add a set volume of deionised water, mix 
to lose the red cells, then add an equal volume of double strength normal 
saline to create a suspension in normal saline.  
The centrifuge where the heavier uncleared cells go to the bottom of the tube.  
I have to say the I have never tried this personally but if the morphology was 
retained for EM it must be fine for LM.
Having said that I have great respect for Tony Henwood and his method looks 
much more scientific.

Regards
Tony

Sent from my iPhone

> On 17 Jan 2020, at 8:24 am, Tony Henwood (SCHN) via Histonet 
>  wrote:
> 
> Hi Jennifer,
> 
> I have had excellent success with lysing the red blood cells (using  Isotonic 
> Ammonium Chloride) prior to cell block preparation with 
> thromboplastin-plasma. 
> The lysing solution contains EDTA so you will need to add a few drops of 1% 
> calcium chloride. Method as follows:
> 
> Lysis solution
> Ammonium Chloride4.5g
> Potassium carbonate  0.5g
> EDTA 0.0186g
> Distilled water  500mls
> 
> Method:
> 1.Centrifuge bloody fluid.
> 2.Remove supernatant and add equal volume of lysis solution.
> 3.Resuspend and incubate for 5 minutes at 4oC.
> 4.Centrifuge, if blood still remains, then repeat from step 2.
> 5. Rinse in Hanks or RPMI, centrifuge.
> 6.Mix pellet in a few drops of plasma.
> 7. Add thromboplastin and a few drops  of 1% Calcium Chloride, mix gently 
> and allow clot to form.
> 8. Add 10% buffered formalin and fix and process as usual.
> 
> Reference:  
> Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479
> 
> If you donot use the plasma clot method for cell block preparation, then use 
> your preferred method after step 5.
> The lysis solution can also be purchased commercially from several companies 
> (eg Biolegend). It is commonly used for sample preparation for flow 
> cytometry. Check the SDS to make sure it does not contain formaldehyde.
> 
> 
> -Original Message-
> From: Mac Donald, Jennifer via Histonet 
> [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Friday, 17 January 2020 4:53 AM
> To: Charles Riley ; Histo List 
> 
> Subject: Re: [Histonet] Cell block preparations
> 
> Acetic acid would work.
> 
> Get Outlook for iOS 
> From: Charles Riley via Histonet 
> Sent: Thursday, January 16, 2020 8:55:21 AM
> To: Histo List 
> Subject: [Histonet] Cell block preparations
> 
>  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.
> 
> What is the best way to remove excess blood from FNA sample collections 
> before spinning them down into cell blocks?
> 
> --
> 
> Charles Riley BS  HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> This message is intended for the addressee named and may contain confidential 
> information. If you are not the intended recipient, please delete it and 
> notify the sender.
> 
> Views expressed in this message are those of the individual sender, and are 
> not necessarily the views of NSW Health or any of its entities.
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet