Hi Kristy,
As the others said, so many things that could be on the way...But making sure
your system is based on a HRP enzyme is definitely a good start, as mentioned
by Joe.
If you can share details, I might be able to help too.
Welcome to the IHC world!
Cheers,
Ana
Ana Maluenda
Research Assistant/Laboratory Manager
Atherothrombosis and Vascular Biology Laboratory
From: Joe Myers
Sent: Saturday, 2 May 2020 4:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC DAB
Kristy:
I’m thinking along the same lines as Paula; is it possible that your detection
reagent contains only Alkaline Phosphatase (ALP) as there a reactive enzyme?
If so, a peroxide/DAB solution simply won’t react with it. Can’t wait to see
your protocol, with detailed descriptions of the antigen, pretreatment reagent
and ‘labeling’ enzyme.
Cheers,
Joe Myers, M.S.,CT/QIHC(ASCP)
From: Kristy Castillo via Histonet
Sent: Friday, May 1, 2020 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC DAB
Hi Histonetters,
We are starting our IHC (fun times), we are having trouble with the DAB
lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent
red is working just fine. Any ideas.
Thank you!
Kristy
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