Hi Kristy,
As the others said, so many things that could be on the way...But making sure your system is based on a HRP enzyme is definitely a good start, as mentioned by Joe. If you can share details, I might be able to help too. Welcome to the IHC world! Cheers, Ana Ana Maluenda Research Assistant/Laboratory Manager Atherothrombosis and Vascular Biology Laboratory ________________________________ From: Joe Myers <jmye...@aol.com> Sent: Saturday, 2 May 2020 4:49 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC DAB Kristy: I’m thinking along the same lines as Paula; is it possible that your detection reagent contains only Alkaline Phosphatase (ALP) as there a reactive enzyme? If so, a peroxide/DAB solution simply won’t react with it. Can’t wait to see your protocol, with detailed descriptions of the antigen, pretreatment reagent and ‘labeling’ enzyme. Cheers, Joe Myers, M.S.,CT/QIHC(ASCP) ________________________________ From: Kristy Castillo via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, May 1, 2020 11:00 AM To: histonet@lists.utsouthwestern.edu<histonet@lists.utsouthwestern.edu> Subject: [Histonet] IHC DAB Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting priv...@baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
