[Histonet] Mart-1 rapid test for Mohs specimens

[Anne Murvosh via Histonet]

I'm working up a price comparison for NovoDiax's rapid 10 minute Mart- 1 stain 
for melanoma on Mohs specimens. The doctors would like me to price shop but I 
only know of 40 minute stains which is too long. Does anyone know of any other 
companies running a rapid 10-15 minute Mart- 1 stain? Thanks Anne Murvosh (HT)
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[Histonet] Bubbles noticed on Bond-Max

[Eddie Martin via Histonet]

Hi Tim, 

From a user perspective: The slides you’re using may be a bad lot causing 
hydrophobic staining or you’re not cleaning your covertiles correctly. The 
wells on the assembly might also not be pulling adequately. Have you cleaned 
the assembly recently, particularly the wells? You could use a clean kit offer 
by Novocastra or make your own. Performing a decontamination on your instrument 
would be helpful too to include your bulk reagent compartments and reagent 
syringe.  Have you also been performing the suggested manufacturer’s guidance 
on maintaining your Bond-Max IHC/ISH analyzer?
Or...
Simply the instrument needs to be recalibrated by a Leica engineer. Do not PM 
with a third party biomed group as the Leica engineer will need to recalibrate 
the instrument with a PM kit to check and if necessary replace the physical 
components, electronics, and fluidic system of your analyzer along with 
updating the coordinate system to match software build according to their 
latest recommendation from their home office. 

Apart from typical lab reagents or processes only performed by an engineer,  do 
you have electrical or magnetic surges in your lab? The backup battery that 
comes with your Bond is typically good for two years, and would need to be 
replaced every few years, especially as the Bond-Max requires a step down from 
the 120V outlet to 110V. And this consideration can also deeply impact your 
overall staining. 


The application specialist should have more politely said that they didn’t know 
but will try to get a response back to you. I don’t comprehend how the tray 
would be the issue unless it was severely cracked and would prevent the slide 
assembly from functioning properly. 

Very respectfully,
Eddie Martin, HT, HTL, QIHC
Hematology Team Leader
The National Institutes of Health
10 Center Drive
Bethesda, MD 20892


Sent from my iPhone

> On Aug 13, 2020, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
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> Today's Topics:
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>   1. Re: End of shift (Charles Riley)
>   2. Blank spots on IHC tissues - bubbles on Bond (Morken, Timothy)
>   3. Re: Blank spots on IHC tissues - bubbles on Bond (Morken, Timothy)
>   4. Re: Blank spots on IHC tissues - bubbles on Bond
>  (Paula Keene Pierce)
>   5. Protocol or SOP of how to make Tissue microarray
>  (Chakib Boussahmain)
> 
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> 
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Re: [Histonet] Blank Spots on IHC- Bubbles on Bond

[Greg Dobbin via Histonet]

Hi Tim,
The usual source of bubbles is a deteriorating syringe or lines or valve.
Have you observed the syringe while running the Clean Fluidics maintenance
function? There can be some "micro" bubbles in the syringe on first draw of
a reagent however, if working properly these tiny bubbles quickly disappear
in subsequent draws of that reagent. If bubbles persist in the barrel of
the syringe throughout the flushing, then you have found the source of the
problem. If the bubbles occur with every reagent then the syringe is the
problem but if you only see bubbles in one of the reagents then it is a
defective valve or line for that reagent.
I hope this was helpful.
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

[Morken, Timothy via Histonet]

Joanna, thanks! You are the first who said they had seen it before. I have a 
Leica tech coming in tomorrow to check out the syringes and lines. That was my 
thought too.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

From: Bartczak, Joanna 
Sent: Thursday, August 13, 2020 10:10 AM
To: Paula Keene Pierce ; Morken, Timothy 
; Histonet 
Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

I have seen this phenomenon years ago on the Bond III when I worked at a 
different laboratory.  If I remember correctly, Leica had to replace the 
syringes and lines as air bubbles were being introduced when aspirating 
reagents.
I would definitely contact Leica technical service and let them investigate.  
Good luck.

Sincerely,
Joanna


Joanna Bartczak

Charge Technologist - Immunopathology
University Health Network - Toronto General Site

200 Elizabeth Street

Toronto, Ontario M5G 2C4

Phone:  (416) 340-4800 ext.6004

joanna.bartc...@uhn.ca




From: Paula Keene Pierce 
mailto:pa...@excaliburpathology.com>>
Sent: Wednesday, August 12, 2020 6:42 PM
To: Morken, Timothy mailto:timothy.mor...@ucsf.edu>>; 
Histonet 
mailto:histonet@lists.utsouthwestern.edu>>
Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

I have seen this when using Sequenza cover plates.
My thoughts were that it is dissolved gases in the washes. As I noticed that 
when filling a container with my tap water and leaving it a while, bubbles form 
on the sides.
Do you brush away bubbles in your water bath even when using distilled water 
too?
Try not using freshly made, stirred, agitated, etc. solutions and adding a 
surfactant.
Extreme solution: place solutions in a vacuum.


Sent from Smallbiz Yahoo Mail for iPhone


On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue 
sections that cause unstained (ie, clear) areas where bubbles have formed?

We've been trying things like extra buffer wash before reagent, new slide trays 
(tech rep suggestion). It is seen on single- antibody slides and very 
noticeable on dual - stain slides on which the first stain is there but the 
second stain shows numerous bubbles that do prevent the second stain from areas 
of the tissue.  We can see the bubble artifact on the slide away from tissue 
sections as well.

Any clues to solve this will be appreciated!
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

[Bartczak, Joanna via Histonet]

I have seen this phenomenon years ago on the Bond III when I worked at a 
different laboratory.  If I remember correctly, Leica had to replace the 
syringes and lines as air bubbles were being introduced when aspirating 
reagents.
I would definitely contact Leica technical service and let them investigate.  
Good luck.

Sincerely,
Joanna


Joanna Bartczak

Charge Technologist - Immunopathology
University Health Network - Toronto General Site

200 Elizabeth Street

Toronto, Ontario M5G 2C4

Phone:  (416) 340-4800 ext.6004

joanna.bartc...@uhn.ca



From: Paula Keene Pierce 
Sent: Wednesday, August 12, 2020 6:42 PM
To: Morken, Timothy ; Histonet 

Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

I have seen this when using Sequenza cover plates.
My thoughts were that it is dissolved gases in the washes. As I noticed that 
when filling a container with my tap water and leaving it a while, bubbles form 
on the sides.
Do you brush away bubbles in your water bath even when using distilled water 
too?
Try not using freshly made, stirred, agitated, etc. solutions and adding a 
surfactant.
Extreme solution: place solutions in a vacuum.


Sent from Smallbiz Yahoo Mail for iPhone


On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet 
 wrote:

Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue 
sections that cause unstained (ie, clear) areas where bubbles have formed?

We've been trying things like extra buffer wash before reagent, new slide trays 
(tech rep suggestion). It is seen on single- antibody slides and very 
noticeable on dual - stain slides on which the first stain is there but the 
second stain shows numerous bubbles that do prevent the second stain from areas 
of the tissue.  We can see the bubble artifact on the slide away from tissue 
sections as well.

Any clues to solve this will be appreciated!
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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use of the intended recipient.
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originally intended is strictly prohibited.
If you have received this e-mail in error, please contact the sender and delete 
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Opinions, conclusions or other information contained in this e-mail may not be 
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