Re: [Histonet] Tissue processor validation

[Simmons, Christopher via Histonet]

Get your gross lab to provide you with samples representative of what you 
normally process and the same for tissue you use for internally procured 
special stain controls (kidney, lung, etc)
Process as usual and cut and perform specials
If they work: done.

Get Outlook for iOS

From: Martha Ward-Pathology via Histonet 
Sent: Wednesday, September 2, 2020 3:51:42 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Tissue processor validation

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue processor validation

[Martha Ward-Pathology via Histonet]

I am posting this question for my colleague in the Histology lab.   She is 
working on a plan for a new tissue processor validation, including special 
stains, and would like to get input from anyone that has already gone through 
it.This is the first time anyone here has ever been involved in validating 
a new processor.   Current ones are 25+ years.


Any help or advice would be appreciated.   Thanks in advance!

Martha Ward, MT (ASCP) QIHC
Wake Forest Baptist Health
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] CAP inspection

[Joe W. Walker, Jr. via Histonet]

The CAP also offers an inspector training course, which provides lots of 
helpful information.  At one time this was a requirement to be a CAP inspector. 
 Not sure about this requirement anymore.

Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology and Interim Phlebotomy Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P 802.747.1790  F 802.747.6525
joewal...@rrmc.org, www.rrmc.org

-Original Message-
From: P Sicurello via Histonet 
Sent: Sunday, August 16, 2020 11:57 AM
To: samantha.gol...@hcahealthcare.com
Cc: HistoNet 
Subject: Re: [Histonet] CAP inspection

[External Email] This email originated from outside of the organization. Think 
before you click: Don’t click on links, open attachments or respond to requests 
for sensitive information if the email looks suspicious or you don’t recognize 
the sender.


Hi Samantha,

Have "fun" with your first inspection.  A few pointers:  try not to compare the 
place you are inspecting with your lab, try to stay focused on the task at hand 
(many new inspectors are easily influenced), walk around and get a feel for the 
lab- sure they are going to be tense because of the CAP inspection - but look 
around - does anything feel off?, SDS's are a good thing to check - many places 
don't update theirs and the SDS is either not current or comes from a different 
vendor than the chemical in use.

You'll probably get lots of suggestions.

Stay safe.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



*Confidentiality Notice*: The information transmitted in this e-mail is 
intended only for the person or entity to which it is addressed and may contain 
confidential and/or privileged material.  Any review, retransmission, 
dissemination or other use of or taking of any action in reliance upon this 
information by persons or entities other than the intended recipient is 
prohibited.  If you received this e-mail in error, please contact the sender 
and delete the material from any computer.


On Sun, Aug 16, 2020 at 8:32 AM Samantha Golden, HT(ASCP) via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> I’m excited to participate in my first CAP audit as part of the
> inspection team, and I would love some tips and advice for a first timer.
>
> I don’t want to get stuck in the weeds as to what I’m searching for.
> While I’ve read all the info from CAP regarding a successful
> inspection, I’d love some first hand experience from those who have done this.
>
> Thanks a bunch.
> Samantha Golden, BS, HT(ASCP)cmQIHCcm
>
>
> ---
> Sent from Workspace ONE
> Boxer __;!!I87qwjxLstg3H_X5!sFgRfeJtvJNEMq7SYM9QIV2bsDfmyJkZrV2JlxKBzv_koq5U
> RGt0Hn0MEXHNHwU$ > ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li
> stinfo/histonet__;!!I87qwjxLstg3H_X5!sFgRfeJtvJNEMq7SYM9QIV2bsDfmyJkZr
> V2JlxKBzv_koq5URGt0Hn0M5MPKz0M$
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!sFgRfeJtvJNEMq7SYM9QIV2bsDfmyJkZrV2JlxKBzv_koq5URGt0Hn0M5MPKz0M$
[https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg]
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Delays in tissue embedding after mouse tissue processing

[Erickson, Jamie E via Histonet]

Hello All,
I have a question about effects on tissues if samples are processed but NOT 
embedding immediately after processing.
We deal with mostly mouse tissues and sometime samples will process over the 
weekend and come off on a day that no one can embed straight away.
Samples are taken out of the processor and let sit on the bench and the 
paraffin coated cassette tissues harden. This delay can be hours or a few days 
if it's a weekend/ holiday.  The samples are then placed in molten paraffin to 
melt and are  embedded.
We have done this with minimal problems but are wondering if some tissues like 
skin mouse ears  could be more difficult due to this delay. Have other lab done 
this delay or have seen any problems

Thank you for any though on this sticky situation...Hehe

Jamie

Jamie ericKson, MS
Scientist II
AbbVie Bioresearch Center, Inc.
Pharmacology/Pathology
100 Research Drive
Worcester, MA 01605
OFFICE+1 508-688-3134
EMAIL  jamie.erick...@abbvie.com
This communication may contain information that is proprietary, confidential, 
or exempt from disclosure. If you are not the intended recipient, please note 
that any other dissemination, distribution, use or copying of this 
communication is strictly prohibited. Anyone who receives this message in error 
should notify the sender immediately by telephone or by return e-mail and 
delete it from his or her computer.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryostats for Mohs specimens

[Woodard, Tonya via Histonet]

Anne,

I have a Avantik QS11 and a QS12 in my Mohs Lab.  The QS12 is a wonderful 
machine with a touch screen for operation and is very user friendly.  I can 
pick up tissue very easily with the QS12 and it works well with freezing 
multiple pieces at a time.  Let me know if you have an other questions about it 
and I would be happy to answer them for you!!!

Tonya?



Tonya K. Woodard

Mohs Histology Specialist

UR Dermatology

40 Celebration Drive
 Rochester, NY 14623
Lab Phone (585)275-9818 / (585)275-9802
Fax (585)334-5823
tonya_wood...@urmc.rochester.edu

Your talent determines what you can do,
Your motivation determines how much you are willing to do,
Your attitude determines how well you do it.
Lou Holtz




___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cryostats for Mohs specimens

[Anne Murvosh via Histonet]

We are shopping for a new cryostat; has anyone used the Tanner Scientific (r) 
TN50 cryostat? Can you let me know if you like it. We are unhappy with the new 
Leica's. We are also looking at the Avantik ones as well. Any input is helpful; 
this is for Mohs specimens so they need to cut well and be able to get a lot of 
tissue on each slide, sometimes the components of the machine get in the way of 
good tissue pick-up. Thanks Anne Murvosh HT
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC validation after a new tissue processer is installed

[Greg Dobbin via Histonet]

Hi martha,
Prognostic markers must be re-validated (Eg.s Breast markers and CD117) as
you described.
Every Ab in your menu should be  tested (as you would for a new a new lot)
and do not forget to validate your H (with various tissue types) and SS
as well. For the H, if possible do side-by-side comparisons between old
and new processors (the downside of what is otherwise an exciting time!).
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet