[Histonet] Fw: Fixative in diff-quick

2021-10-05 Thread Tony Henwood (SCHN) via Histonet 
Hi Clay,

The first solution is methanol.
So lab grade methanol will do.
The blue colour is from an innocuous dye added to differentiate from water (and 
ethanol used in cytology fixation). This helps us poor cytologists when we are 
doing ROSE at FNAs.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-Original Message-
From: Corbin, Clay via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, 5 October 2021 7:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fixative in diff-quick

Hey folks,
I am shopping for a diff-quick kit.  However, all I really need is the 
fixative.  Generally, there is a blue stain (triarylmethane) added to the 
methanol in the fixative solution.  I have a giant jug of lab grade methanol.  
What would I lose by using methanol alone compared to the fixative solution 
included in a diff-quick kit?
Thanks!
Clay

Clay Corbin, PhD
Professor of Biology
Bloomsburg University
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Re: [Histonet] FW: Biopsy processing

2021-10-05 Thread John Garratt via Histonet 
We (CPQA) ran our first EQA to included a review of tissue processing and 
staining of various tissue and the report might be of interest to you and 
others. Contact me directly if you, or anybody else, would like a copy of the 
report.

John

Sent from ProtonMail for iOS

On Tue, Oct 5, 2021 at 9:30 AM, Hannen, Valerie via Histonet 
 wrote:

> From: Hannen, Valerie
> Sent: Monday, October 04, 2021 2:54 PM
> To: 'histo...@list.utsouthwestern.edu'
> Subject: FW: Biopsy processing
>
> From: Hannen, Valerie
> Sent: Monday, October 4, 2021 11:03 AM
> To: 'histo...@list.utsouthwestern.edu' 
> Subject: Biopsy processing
>
> Good Morning All... My Pathologist is wondering if there is any benefit to 
> having a separate processing run just for small biopsies? He said that what 
> is seeing now, he has no issue with, the sections are just as good as all the 
> other tissue sections. He just asked for me to get the consensus of the group.
>
> Thank You in advance!!
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333 Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
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[Histonet] Using LR white with a tungsten carbide knife

2021-10-05 Thread Chaitanya Kolluru via Histonet 
Hi,

I’m looking for some advice on using LR white on an osmium stained tissue.
Unfortunately, I don’t have access to an ultra-microtome or diamond knives.

Has someone here tried sectioning LR white with tungsten carbide knives on
a motorized rotary microtome? Are there any sectioning artifacts that I
might see? Also, LR white seems to be available in three grades  - soft,
medium and hard. Is the soft grade recommended with my setup?

Thank you,
Chaitanya
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Re: [Histonet] Histonet Digest, Vol 215, Issue 3

2021-10-05 Thread John Frazier via Histonet 
If you are looking to prioritize certain tissues slides to the pathologist,
I think separate small biopsy runs are a great idea. You can process them
with a much shorter run cycle.(90-120 min). You can actually do several
runs per day. This is a good way of decreasing your TAT for all blocks and
putting the signout TAT back in the pathologist's hands. If you are
interested, I can suggest a run schedule.


*John Frazier*
*MT(ASCP), MBA, *
*Lean 6 Six Sigma Black Belt*
*Healthcare Consultant*
*C - 302-310-7567*
*H - 704-847-0566*
*wilfong1...@gmail.com *






On Tue, Oct 5, 2021 at 1:00 PM 
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>1. Re: Dissect Aid - Histonet Digest, Vol 215, Issue 2 (Terri  Braud)
>2. Fixative in diff-quick (Corbin, Clay)
>3. Re: Fixative in diff-quick (Bryan Llewellyn)
>4. Tissue Quality (Chakib Boussahmain)
>5. microtome (Silvia Bonner)
>6. FW: Biopsy processing (Hannen, Valerie)
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> Subject: Re: [Histonet] Dissect Aid - Histonet Digest, Vol 215, Issue 2
> We do not re-use.
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> Subject: [Histonet] dissect aid
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> Happy Monday!
> For those using Dissect Aid (Statlab) - are you re-using this product for
> multiple specimens?
> Can you please share what your process is for this?
> Much appreciated,
> Nancy Schmitt MLT, HT(ASCP)
> MercyOne Dubuque
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> -- Forwarded message --
> From: "Corbin, Clay" 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Mon, 4 Oct 2021 20:58:17 +
> Subject: [Histonet] Fixative in diff-quick
> Hey folks,
> I am shopping for a diff-quick kit.  However, all I really need is the
> fixative.  Generally, there is a blue stain (triarylmethane) added to the
> methanol in the fixative solution.  I have a giant jug of

[Histonet] FW: Biopsy processing

2021-10-05 Thread Hannen, Valerie via Histonet 



From: Hannen, Valerie
Sent: Monday, October 04, 2021 2:54 PM
To: 'histo...@list.utsouthwestern.edu'
Subject: FW: Biopsy processing



From: Hannen, Valerie
Sent: Monday, October 4, 2021 11:03 AM
To: 'histo...@list.utsouthwestern.edu' 
Subject: Biopsy processing

  Good Morning All...  My Pathologist is wondering if there is any benefit 
to having a separate processing run just for small biopsies?  He said that what 
is seeing now, he has no issue with, the sections are just as good as all the 
other tissue sections. He just asked for me to get the consensus of the group.

Thank You in advance!!




Valerie A. Hannen,MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Avenue
Titusville, Florida 32796
P: 321-268-6333  Ext. 7506
F: 321-268-6149
valerie.han...@parrishmed.com
www.parrishmed.com

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[Histonet] microtome

2021-10-05 Thread Silvia Bonner via Histonet 
Hello,

We are in the market for a new/refurbished microtome.  We currently have a 
Micron HM355s, and Micron HM 335E.  We are interested in buying something 
comparable.  Any suggestions?  We are looking at Micron and Leica.  Good or bad 
reviews would be appreciated.

Thanks,


Silvia Bonner, BS, HT(ASCP) CM

Histology Supervisor



sbon...@pathregional.com

Pathologists’ Regional Laboratory

Anatomical Pathology

2841 Juniper Dr. Suite 1

Lewiston, ID 83501

(208)746-5533



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[Histonet] Tissue Quality

2021-10-05 Thread Chakib Boussahmain via Histonet 


Hi Guys,

We normally buy human tissue blocks to use them as controlsor to develop IF 
assays in our laboratory. However, most of these blocksappeared to be fixed or 
processed adequately. I am wondering if anybody has amethod to assess the 
quality of these tissue blocks besides H stain. Forinstance, is there an IHC 
marker or a panel of the markers that I can use as agood indicator to qualify 
these tissue blocks?

Thank you so much in advance for any suggestions!

Best

Chakib

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