Re: [Histonet] Alcian blue van gieson double stain

[Tony Reilly via Histonet]

Hi Charles

I have done this stain many times as an abbreviated version of Movats 
Pentachrome.
You are correct in thinking the van gieson will affect the AB stain due to the 
acid.

As in the Movat stain do AB first then place slides in alcoholic ammonia for 1 
hour to convert AB to insoluble Monastral blue then finish with the VVG.

Regards
Tony Reilly

Sent from my iPhone

> On 10 Mar 2023, at 5:49 am, Akemi Allison via Histonet 
>  wrote:
> 
> Just curious, why not do a Movat’s Pentachome? It gives you everything you 
> are looking for and more.  
> Best regards,
> Akemi Allison-Tacha, BS, Ht, HTL 
> akemiat3...@gmail.com
> 
> Sent from my iPhone
> 
>> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet 
>>  wrote:
>> 
>> Hi,
>> if they are wanting to stain elastic, Alcian blue, and Van Gieson, I 
>> recommend doing the Verhoff elastic stain first, then the Alcian blue, and 
>> then the Van Gieson.
>> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
>> Blue Lake DriveNorman, OK 73069PH 
>> 405-759-3953http://www.excaliburpathology.com
>> 
>> A sharp knife is nothing without a sharp eye. - Klingon Proverb 
>> 
>>   On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet 
>>  wrote:  
>> 
>> I am working with a researcher and they saw an article where someone
>> performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
>> has done this before can you provide your protocol.  I am trying to figure
>> out the best way to perform this process to get the best staining results.
>> Here are my questions/thoughts
>> 
>> 1. Is it better to run the Alcian blue as usual and then perform the van
>> gieson stain?
>> 2. Will the differentiating solution in the van gieson stain affect the
>> alcian blue staining in any way?
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Re: [Histonet] Alcian blue van gieson double stain

[Akemi Allison via Histonet]

Just curious, why not do a Movat’s Pentachome? It gives you everything you are 
looking for and more.  
Best regards,
Akemi Allison-Tacha, BS, Ht, HTL 
akemiat3...@gmail.com

Sent from my iPhone

> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet 
>  wrote:
> 
> Hi,
> if they are wanting to stain elastic, Alcian blue, and Van Gieson, I 
> recommend doing the Verhoff elastic stain first, then the Alcian blue, and 
> then the Van Gieson.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
> Blue Lake DriveNorman, OK 73069PH 
> 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb 
> 
>On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet 
>  wrote:  
> 
> I am working with a researcher and they saw an article where someone
> performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
> has done this before can you provide your protocol.  I am trying to figure
> out the best way to perform this process to get the best staining results.
> Here are my questions/thoughts
> 
> 1. Is it better to run the Alcian blue as usual and then perform the van
> gieson stain?
> 2. Will the differentiating solution in the van gieson stain affect the
> alcian blue staining in any way?
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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Re: [Histonet] Alcian blue van gieson double stain

[Paula Keene Pierce via Histonet]

Hi,
if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend 
doing the Verhoff elastic stain first, then the Alcian blue, and then the Van 
Gieson.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet 
 wrote:  
 
 I am working with a researcher and they saw an article where someone
performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
has done this before can you provide your protocol.  I am trying to figure
out the best way to perform this process to get the best staining results.
Here are my questions/thoughts

1. Is it better to run the Alcian blue as usual and then perform the van
gieson stain?
2. Will the differentiating solution in the van gieson stain affect the
alcian blue staining in any way?
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[Histonet] Alcian blue van gieson double stain

[Charles Riley via Histonet]

I am working with a researcher and they saw an article where someone
performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
has done this before can you provide your protocol.  I am trying to figure
out the best way to perform this process to get the best staining results.
Here are my questions/thoughts

1. Is it better to run the Alcian blue as usual and then perform the van
gieson stain?
2. Will the differentiating solution in the van gieson stain affect the
alcian blue staining in any way?
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Re: [Histonet] Mice/Rat brain

[Kurth, Virginia L via Histonet]

I remember adding a couple drops of ETOH to the water bath; it's been a while 
so I am not sure the exact protocol.  (it will spin the tissue).  

Virginia Kurth, HT, (ASCP)CM
UW Health Surgical Pathology
Madison, WI  53792




 

-Original Message-
From: Renee Fisher via Histonet  
Sent: Thursday, March 9, 2023 8:51 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mice/Rat brain

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

We are having issues with mice and rat brain sections. There are folds in the 
tissue. We just can't seem to get a flat section without folds. Any suggestions?
Sent from my iPhone
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[Histonet] Georgia Society for Histotechnology 50th Anniversary

[Shirley A. Powell via Histonet]

Hi Histotechs,
Please join us at Sea Palms Resort on St Simons Island, GA for our 50th 
anniversary meeting.  The links to the program and registration are below.
Rooms will go fast so make your reservations soon.  The hotel rates and phone 
number to call are on our website at 
www.georgiahistotech.org
These are the Program Schedule link and the Registration Form link.

50th Anniversary Program 
Schedule

50th Anniversary Registration 
Form

Look forward to seeing ya'll there.
Shirley
Shirley Powell, HTL(ASCP)
Technical Director Histology Curricular Support Laboratory
Pathology Department
Mercer University School of Medicine
powell...@mercer.edu


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Re: [Histonet] Mice/Rat brain

[Paula Sicurello via Histonet]

Hi Renee, 
Brains are notorious for being difficult.  Processing has to be just so, 
embedding has to be just so, and sectioning?  Well, just plain tricky.
It sounds like your water bath may be a smidge too cool.  Brains are picky that 
way.  Water bath too warm?  Poof!  The section explodes.  Too cool? Wrinkles.  
Can you use vibratome sections or do you need FFPE sections?

Paula Sicurello 
 
  On Thu, Mar 9, 2023 at 6:51 AM, Renee Fisher via 
Histonet wrote:   
We are having issues with mice and rat brain sections. There are folds in the 
tissue. We just can’t seem to get a flat section without folds. Any suggestions?
Sent from my iPhone
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[Histonet] Mice/Rat brain

[Renee Fisher via Histonet]

We are having issues with mice and rat brain sections. There are folds in the 
tissue. We just can’t seem to get a flat section without folds. Any suggestions?
Sent from my iPhone
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