Re: [Histonet] Modified Davidson solution

2024-03-06 Thread Colleen Forster via Histonet 
I agree. Making some of these simple solutions that need to be FRESH is the
better way to go. As John said, there are ma ny books with these formulas
in them.

I have used Davidson's for different projects and I make mine in the lab
fresh each time tissue is harvested.

Colleen Forster HT(ASCP)QIHC

On Wed, Mar 6, 2024 at 5:26 AM Tony Henwood via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I agree.
>
> A great book.
>
>
> Regards,
>
>
>
> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>
> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>
> Adjunct Fellow, School of Medicine, University of Western Sydney.
>
> 
> From: John Kiernan via Histonet 
> Sent: 06 March 2024 18:32
> To: Histonet ; Naira Margaryan <
> nmargarya...@gmail.com>; Davoli, Katherine A 
> Subject: Re: [Histonet] Modified Davidson solution
>
> The Davidson/Hartmann fixative is just another alcoholic formaldehyde
> mixture acidified with acetic acid. Like all others of that ilk it was
> intended for making up in the lab soon before using. Storage causes slow
> deterioration. The ethanol slowly gets esterified by the acetic acid,
> making ethyl acetate, which has no fixative properties. This change is
> evident from the fruity smell of the ester, which becomes evident after a
> week or two. All this has been in the textbooks for more than 50 years.
> Why doesn't every lab spend perhaps $200 on a couple of books?  For
> obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from
> the publisher, which is $30 less than Amazon's price), but there are other
> histotechnology books that are very good.  A book may cost much less than a
> big bottle of a deteriorating mixture of questionable value, and you and
> your staff could learn a lot by reading.
> John Kiernan
> = = =
> 
> From: Davoli, Katherine A via Histonet 
> Sent: March 5, 2024 4:40 PM
> To: Histonet ; Naira Margaryan <
> nmargarya...@gmail.com>
> Subject: Re: [Histonet] Modified Davidson solution
>
> Hi Naira, I get Hartmann's from Electron Microscopy Sciences
> Cat# 64133-10 (for 1L, but they have larger sizes I believe)
>
>
> Katherine Davoli, HTL(ASCP)cm(they/them/theirs)
>
> Lab Manager, Tissue Culture and Histology Core Facilities
>
> U. Pitt Dept of Ophthalmology
>
> 7.373 UPMC Mercy Pavilion
>
> 1622 Locust St., Pittsburgh PA 15219
>
> (412) 624-8508   this number cannot receive texts
>
> 
> From: Naira Margaryan via Histonet 
> Sent: Tuesday, March 5, 2024 1:27 PM
> To: Histonet 
> Subject: [Histonet] Modified Davidson solution
>
> Hello!
>
> What is the best company to buy a ready to use Modified Davidson solution?
>
> Thanks in advance,
> Naira
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> 
> From: Davoli, Katherine A via Histonet 
> Sent: March 5, 2024 4:40 PM
> To: Histonet ; Naira Margaryan <
> nmargarya...@gmail.com>
> Subject: Re: [Histonet] Modified Davidson solution
>
> Hi Naira, I get Hartmann's from Electron Microscopy Sciences
> Cat# 64133-10 (for 1L, but they have larger sizes I believe)
>
>
> Katherine Davoli, HTL(ASCP)cm(they/them/theirs)
>
> Lab Manager, Tissue Culture and Histology Core Facilities
>
> U. Pitt Dept of Ophthalmology
>
> 7.373 UPMC Mercy Pavilion
>
> 1622 Locust St., Pittsburgh PA 15219
>
> (412) 624-8508   this number cannot receive texts
>
> 
> From: Naira Margaryan via Histonet 
> Sent: Tuesday, March 5, 2024 1:27 PM
> To: Histonet 
> Subject: [Histonet] Modified Davidson solution
>
> Hello!
>
> What is the best company to buy a ready to use Modified Davidson solution?
>
> Thanks in advance,
> Naira
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN

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Re: [Histonet] Modified Davidson solution

2024-03-06 Thread Tony Henwood via Histonet 
I agree.

A great book.


Regards,



Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)

Principal Scientist, the Children’s Hospital at Westmead (Retired)

Adjunct Fellow, School of Medicine, University of Western Sydney.


From: John Kiernan via Histonet 
Sent: 06 March 2024 18:32
To: Histonet ; Naira Margaryan 
; Davoli, Katherine A 
Subject: Re: [Histonet] Modified Davidson solution

The Davidson/Hartmann fixative is just another alcoholic formaldehyde mixture 
acidified with acetic acid. Like all others of that ilk it was intended for 
making up in the lab soon before using. Storage causes slow deterioration. The 
ethanol slowly gets esterified by the acetic acid, making ethyl acetate, which 
has no fixative properties. This change is evident from the fruity smell of the 
ester, which becomes evident after a week or two. All this has been in the 
textbooks for more than 50 years.
Why doesn't every lab spend perhaps $200 on a couple of books?  For obvious 
reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from the 
publisher, which is $30 less than Amazon's price), but there are other 
histotechnology books that are very good.  A book may cost much less than a big 
bottle of a deteriorating mixture of questionable value, and you and your staff 
could learn a lot by reading.
John Kiernan
= = =

From: Davoli, Katherine A via Histonet 
Sent: March 5, 2024 4:40 PM
To: Histonet ; Naira Margaryan 

Subject: Re: [Histonet] Modified Davidson solution

Hi Naira, I get Hartmann's from Electron Microscopy Sciences
Cat# 64133-10 (for 1L, but they have larger sizes I believe)


Katherine Davoli, HTL(ASCP)cm(they/them/theirs)

Lab Manager, Tissue Culture and Histology Core Facilities

U. Pitt Dept of Ophthalmology

7.373 UPMC Mercy Pavilion

1622 Locust St., Pittsburgh PA 15219

(412) 624-8508   this number cannot receive texts


From: Naira Margaryan via Histonet 
Sent: Tuesday, March 5, 2024 1:27 PM
To: Histonet 
Subject: [Histonet] Modified Davidson solution

Hello!

What is the best company to buy a ready to use Modified Davidson solution?

Thanks in advance,
Naira
___
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From: Davoli, Katherine A via Histonet 
Sent: March 5, 2024 4:40 PM
To: Histonet ; Naira Margaryan 

Subject: Re: [Histonet] Modified Davidson solution

Hi Naira, I get Hartmann's from Electron Microscopy Sciences
Cat# 64133-10 (for 1L, but they have larger sizes I believe)


Katherine Davoli, HTL(ASCP)cm(they/them/theirs)

Lab Manager, Tissue Culture and Histology Core Facilities

U. Pitt Dept of Ophthalmology

7.373 UPMC Mercy Pavilion

1622 Locust St., Pittsburgh PA 15219

(412) 624-8508   this number cannot receive texts


From: Naira Margaryan via Histonet 
Sent: Tuesday, March 5, 2024 1:27 PM
To: Histonet 
Subject: [Histonet] Modified Davidson solution

Hello!

What is the best company to buy a ready to use Modified Davidson solution?

Thanks in advance,
Naira
___
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
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