Re: [Histonet] KP Marker Plus

2009-05-15 Thread APRIL SHOWERS
> Does anyone know who sells the KP Marker PLUS? I can't seem to find them.
Thanks for any help.

Did you try Googling it?

http://www.google.com/#hl=en&q=KP+Marker+Plus&btnG=Google+Search&aq=f&oq=KP+Marker+Plus&fp=-dx2DTn7Pl4






On Fri, May 15, 2009 at 12:12 PM, Metzger, Kenneth <
kenneth.metz...@aruplab.com> wrote:

> Does anyone know who sells the KP Marker PLUS? I can't seem to find
> them. Thanks for any help.
>
>
>
> Ken
>
>
>
> Kenneth G Metzger HTL(ASCP)
>
> ARUP Labs
>
> Histology Supervisor
>
> 500 Chipeta Way
>
> Salt Lake City, Utah 84108-1221
>
> kenneth.metz...@aruplab.com
>
> (801) 583-2787 x 3101
>
>
>
>
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Re: [Histonet] 70% alcohol fixation of brain

2009-05-15 Thread APRIL SHOWERS
> Good luck freezing a brain that is full of alcohol! Have you checked the
freezing point of alcohol?
> Why are you doing this?

I think that moire often than not, people ask before they think ;-)



On Fri, May 15, 2009 at 11:56 AM, Geoff McAuliffe wrote:

> Greetings TF:
>
> Good luck freezing a brain that is full of alcohol! Have you checked the
> freezing point of alcohol?
> Why are you doing this?
> Immersing a whole brain in 70% alcohol, why??
> 30% sucrose is a cryoprotectant so the brain is not full of holes from ice
> crystals.Alcohol defeats this purpose.
>
> Geoff
>
>
>
> TF wrote:
>
>> Hi, i dont want to use PFA for the brain fixation (rat).
>> Now I tried to perfuse the rat with saline, followed with 70% alcohol.
>> Then I do post-fixation at room temperature for 24 hours.
>> I also tried saline perfusion, then I directly put the whole brain into
>> 70% alcohol.
>> Is this fine?
>>
>> Also, for dehydration before cutting frozen sections on a
>> cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~
>>
>> 2009-05-15
>>
>>
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>>
>
>
> --
> --
> **
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583 mcaul...@umdnj.edu
> **
>
>
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Re: [Histonet] Customary fees

2009-05-15 Thread APRIL SHOWERS
> It seems that people are suing for any and everything they can these days

I would too if I was misdiagnosed or otherwise the victim of malpractice
involving incorrect/erroneous histopathology. Don't disrespect such victims
by lumping them in the "damn litigious people these days!" crowd.





On Fri, May 15, 2009 at 11:44 AM, Mike Pence  wrote:

> Hi All,
>
> I am just wanting to get an idea of how many of you charge law firms or
> legal gathering sites for making copies of pathology reports and sending
> slides and blocks. It seems that people are suing for any and everything
> they can these days. I would also like to know what people are charging
> for their copying and time with these cases. Please respond off line if
> you don't want to "publish" your fees.
>
> Thanks,
> Mike Pence
> AP Supervisor
> GRMC
> West Burlington, Iowa
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Re: [Histonet] CD25

2009-05-15 Thread APRIL SHOWERS
Google is always a good first step:
http://www.google.com/#hl=en&q=CD25+in+canine+tissue&btnG=Google+Search&aq=f&oq=CD25+in+canine+tissue&fp=-dx2DTn7Pl4

On Fri, May 15, 2009 at 4:02 AM, NEIL MACINTYRE <
neil.macint...@btinternet.com> wrote:

> Hi everyone
>
> I have a researcher wishing to look at CD25 in canine tissue. Has anyone
> any information that could help?
>
> Many thanks
>
> Neil MacIntyre
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Re: [Histonet] Embeeding frozen tissue in paraffin

2009-05-15 Thread APRIL SHOWERS
> is there any good method for embeeding fresh frozen tissue (without any
fixation) in paraffin?

Wouldn't the molten paraffin would melt the frozen tissue? LOL
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