[Histonet] Microsporidia
Dear Friends, Can anyone tell me how to demonstrate Micrsporidia in Deudenal biopsy.For me the modified Brown and bren gram is not working.if u have any other method pls let me know. with regrads, Aazath ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAP
Dear friends, does anyone have rapid PAP(cytostain) preparation,for rapid PAP staining. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals chennai India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 80, Issue 31
Dear Friends, Does anyone have rehydration technique of air dried smear which can be stained for pap. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals -chennai India ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Artifacts in histology section
Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problem in IF stains
Dear Friends, I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative. Can you people tell me the possible mistake which may cause these kind of effects on the IF stain. I dont see any gross mistakes on the Frozen sections after sectioning.All the temperature and other things are fine on the cryostate, but my pathologist feels that there may be any problem in section.will that have any effects on the IF stains.. Aazathraj. Technical Officer. Apollo Hospitals India. _ The latest songs, trailers and more http://video.in.msn.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fading of Histology slides
Dear Colleagues , I am an Histopathology Technologist .We use DPX (merck) as the mounting medium for the the light microscopy slides. Particularly HE slides gets faded very fast with min 3 -4 month. Is there any other alternative other than gum dammar which we are not getting now a days in the market. Can any one suggest me with some way to avoid the fading of HE slides. With regards, Aazathraj.P Technical Officer, Department of Histopathology and Cytology Apollo Hospitals-Chennai India _ Your E-mail and More On-the-Go. Get Windows Live Hotmail Free. https://signup.live.com/signup.aspx?id=60969___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing Of CSF
Dear Friends, Iam a Technical Officer in Apollo Hospitals India in Department of Histopathology and Cytology. I am having a problem in CSF processing for cytology. We are using Thermo Shandon 4- Cytocentrifuge for processing CSF and Urine. The yield is very less. We spin it with 750rpm ( acceleration- medium)for 5 min with 1drop of egg albumin as adisive.Most of the time the CSF has been reported as no cellular material found.Iam little concern about the technique ,which we are following. Is anybody have a better way to process the CSF for Cytology with better yield of material. Merry X-Mas and Happy New Year to all. with regards, Aazath _ Windows 7: Find the right PC for you. Learn more. http://windows.microsoft.com/shop___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE preparation
Dear All, We are preparing harris heamtoxylin for HE in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India _ News, sports, entertainment and fine living…learn the ropes on MSN India http://in.msn.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Dear All, we have got a new leica multistainer ST 5020.we are using it for both HE and as well as PAP.Out of 34 station i have only 15 station for HE.Is anybody having a Staining protocol for HE which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo Hospitals Chennai India _ Log on to MSN India for a lowdown on what’s hot in the world today http://in.msn.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
