[Histonet] Teaching

[Abbott, Tanya via Histonet]

I am looking for any information anyone can offer regarding using/teaching 
Histology techniques in a high school setting. Any input is greatly 
appreciated! Feel free to email me, thanks!
Tanya Abbott   arh...@yahoo.com


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[Histonet] Decal

[Abbott, Tanya via Histonet]

Just wondering if anyone has switched to a different decal and done any sort of 
validation? We are CAP accredited.
Thanks in advance,
Tanya

Tanya G. Abbott, HT (ASCP)CM, MLT(CSMLS)
Pathology Manager


Penn State Health St. Joseph
Pathology Department
(o) 610-378-2635
(f) 610-898-5871
tabb...@pennstatehealth.psu.edu
Penn State Health St. Joseph | The Future of 
Healthcare


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[Histonet] Technical/Performing

[Abbott, Tanya via Histonet]

We currently have a "performing location" listed on our reports. CAP is telling 
me that if you have technical work at one lab, and sign out/Interp at another 
lab, you must list both on the report.
We are a multiple facility where my lab does our own work, but will be 
performing technical work for another hospital in our health system. I also 
have a bigger hospital that will be doing this for different facilities. So 5 
facilities in total.
Thoughts on how to handle this? We are thinking a generic disclaimer but it's 
going to be lengthy! Hope my explanation made sense?!

Tanya G. Abbott, HT (ASCP)CM, MLT(CSMLS)
Pathology Manager

Penn State Health St. Joseph
Pathology Department
(o) 610-378-2635
(f) 610-898-5871
tabb...@pennstatehealth.psu.edu
Penn State Health St. Joseph | The Future of 
Healthcare


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[Histonet] Giemsa controls

[Abbott, Tanya via Histonet]

Suggestions for where to buy good Giemsa controls for H. pylori? Thank you in 
advance!
Tanya



Tanya G. Abbott
Pathology Manager
PennState Health St. Joseph
Reading Pennsylvania
tabb...@pennstatehealth.psu.edu
610-378-2635


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[Histonet] Job posting

[Abbott, Tanya via Histonet]

St. Joseph Hospital in Reading, PA has an immediate opening for a Full-time 
Histotechnologist.

JOB SUMMARY:
The Histotechnologist at St. Joseph Hospital is responsible for performing 
technical duties in preparation of surgical specimens for microscopic 
examination.

RESPONSIBILITIES:
Receive and accession tissue specimens and prepare specimens for processing.
Cut thin sections and stain according to established procedures for routine, 
special stains, immunohistochemistry and coverslip and label finished slides 
for the pathologists review.
Assist the pathologist with cutting and staining of tissues for frozen sections.
Maintain equipment, instruments and cleanliness of working area and maintain a 
safe, clean work environment including proper labeling and storage of chemicals 
and specimens (and disposal of same).
Prepares written documentation as required by regulatory agencies, the 
profession and the department. Regulatory agencies include, but are not limited 
to, FDA, JCAHO, CAP, CLIA, OSHA and the state in which this facility is located.

MINIMUM QUALIFICATIONS:

Associates degree required
At least two years on-the-job training or experience is required
Experience with personal computers is required

PREFERRED QUALIFICATIONS:

Bachelors degree preferred
Certified as HTL/HT (ASCP) or equivalent preferred or eligible for 
certification as HTL/HT (ASCP)

St. Joseph Hospital offers an exceptional benefit package including but not 
limited to:

* Health, Vision, and Dental plans effective first day of hire
* Wellness programs
* Employee discounts
* Generous Paid time off for work/life balance

About St. Joseph Hospital:
Located in the heart of Berks County in Reading, PA, St. Joseph Medical Center 
first opened its doors in 1873. The new 204 bed state of the art hospital, 
located on Bernville Road, opened in 2004 and in 2015 became the first hospital 
to join the Penn State Health organization.  Penn State Health St. Joseph is 
dedicated to the health, education and wellness of the community.

To apply online, visit our career site:  careers.pennstatehealth.org
Job #16924

Thank you!

Tanya G. Abbott
Pathology Manager
PennState Health St. Joseph
Reading Pennsylvania
tabb...@pennstatehealth.psu.edu
610-378-2635


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[Histonet] Tissue discard

[Abbott, Tanya via Histonet]

What sort of PPE does everyone use when "dumping" tissue? Interested mostly in 
if folks are using a N95 mask, respirator, PAPR? We pour the formalin off our 
tissues and bag the tissues to be taken away.
Any input is appreciated!
Tanya

Tanya G. Abbott, HT (ASCP)CM, RT(CSMLS)
Pathology Manager
Penn State Health St. Joseph
Reading, PA
tabb...@pennstatehealth.psu.edu


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[Histonet] Primera cassette engraver

[Abbott, Tanya via Histonet]

Has anyone out there set up a Primera cassette engraver with CoPath? If so, 
could you reach out to me please, I have some questions!! Any help is greatly 
appreciated!!!
Best,
Tanya


Tanya G. Abbott
Pathology Manager
PennState Health St. Joseph
Reading Pennsylvania
tabb...@pennstatehealth.psu.edu
610-378-2635


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[Histonet] Temporary Histotech position

[Abbott, Tanya via Histonet]

I am in need of a temporary Histotechnologist from approximately March 3 for 8 
to 10 weeks, any interest, please contact me, thanks!
Tanya

Tanya G. Abbott, HT (ASCP)CM, RT(CSMLS)
Pathology Manager

Penn State Health St. Joseph
Pathology Department
(o) 610-378-2635
(f) 670-898-5871
tabb...@pennstatehealth.psu.edu
Penn State Health St. Joseph | The Future of 
Healthcare

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[Histonet] TempTrak monitoring system

[Abbott, Tanya via Histonet]

Good morning!
Is anyone using the TempTrak automated temperature monitoring system in their 
Lab? We are getting ready to go live with it, but are having problems with 
TempTrak probes reading the proper temperatures for our cryostats. And also a 
-30C refrigerator in another part of the Lab. The system doesn't seem to like 
cold temps! Any input would be greatly appreciated! Tanya



Tanya G. Abbott
Pathology Manager
PennState Health St. Joseph
Reading Pennsylvania
tabb...@pennstatehealth.psu.edu
610-378-2635


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[Histonet] Cassette/slide printers

[Abbott, Tanya via Histonet]

Looking for suggestions on cassette and slide printers (either direct print or 
"paper" labels for slides) for a smaller, community hospital lab.
Histo - 2 cutting stations, I Grossing area, average about 150 slides, 100 
cassettes/day
Cyto - average 40 ThinPreps, 5 FNAs/day
Any input is appreciated, thanks in advance!
Tanya

Tanya G. Abbott HT(ASCP), RT(CSMLS)
Pathology Manager
Penn State Health St. Joseph
Pathology Department
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[Histonet] Medicare 14 day rule

[Abbott, Tanya via Histonet]

I am just curious on  how everyone is handling the Medicare 14 day rule when 
sending blocks out to reference labs for testing?
Are you just holding on to those blocks for 14 days post discharge for 
inpatients then sending out? Reregistering these with the
Date of Service as when you are sending these blocks out? Any input is 
appreciated!
Best,
Tanya

Tanya G. Abbott
Pathology Manager
PennState Health St. Joseph
Reading Pennsylvania

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[Histonet] Bar Code and Tracking

[Abbott, Tanya via Histonet]

Hello,
I manage a small Pathology lab where we generate approx. 80-140 blocks for 
Histo and 5-6 Cytology fluids a day. I have 2 Histotechs that swap doing 
grossing monthly. I have 1 full time and 1 part time Cytotech and a Cyto 
assistant. I am curious if there are any smaller labs out there that have gone 
to a Bar Code and Tracking system and can offer any input. I came from a much 
larger lab that used CoPath from accessioning to sign-out, so that is the kind 
of system I am used to. It is certainly a system I wish to integrate in to my 
lab on a much smaller level, we currently have a very manual process, other 
than some entry/labeling in our LIS.
Any input is appreciated! Tanya

Tanya G. Abbott
Pathology Manager
Penn State Health St. Joseph
Pathology Department
(o) 610-378-2635
(f) 670-898-5871
tabb...@pennstatehealth.psu.edu
Penn State Health St. Joseph | The Future of 
Healthcare

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[Histonet] New CPT Code 88350

[Abbott, Tanya via Histonet]

Has anyone determined how/when to use the new CPT code 88350 for 
Immunofluorescent studies? Is it like IHC? First IHC is 88342, the rest are 
88341. So the  first IF is 88346, the rest ordered are 88350?
Thanks in advance for your input!
Tanya

Please note new email address: 
tabb...@pennstatehealth.psu.edu, 
effective immediately

Tanya G. Abbott
Pathology Manager - Histology/Cytology
Penn State Health St. Joseph
Phone:610-378-2635
Fax: 610-898-5871




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[Histonet] VIP5

[Abbott, Tanya via Histonet]

Help!!! We changed our processor on Monday, all reagents. Run Monday into 
Tuesday a.m. was fine. Tuesday nights run had some blocks in it that weren't 
fully processed; chamber looked like it had droplets of residual water (or 
maybe alcohol?) in it. Dumped and refilled everything, ran a few test blocks, 
same scenario.
Any suggestions?!
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
Penn State Health St. Joseph
(phone) 610-378-2635

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[Histonet] Coverslip tape

[Abbott, Tanya via Histonet]

This is more a Cyto question, but, does anyone out there use the Sakura 
Coverslip tape to coverslip their Thin Preps? We seem to be having a problem 
down the sides of the slide where the tape is not being pushed down and access 
glue seems to accumulate there. Sakura tells us it's a drying issue but I 
really don't think it is, we let the slides dry long enough. It seems to me 
that the bar that pushes the tape down is not pushing down well enough on the 
sides. We have changed the springs recently and are on a new roll of tape. Our 
H slides don't seem to be affected.
Any input is appreciated! Happy Friday!
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
Penn State Health St. Joseph
(phone) 610-378-2635

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[Histonet] Grossing Histotechs

[Abbott, Tanya via Histonet]

So, are there a lot of Histotechs out there who are still grossing? Or is this 
job being done by PAs?
Thanks! Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
Penn State Health St. Joseph
(phone) 610-378-2635

This email and attachments contain information that may be confidential or 
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[Histonet] Slide label printer

[Abbott, Tanya via Histonet]

I am looking for a slide label printer that is compatible with Meditech, any 
suggestions? If so, also any suggestions on what label stock/size to use that 
obviously can go through chemicals/staining?
Thanks in advance! Tanya


Tanya G. Abbott
Manager Technologist
Histology/Cytology
Penn State Health St. Joseph
(phone) 610-378-2635

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
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[Histonet] IHC negative controls

[Abbott, Tanya via Histonet]

Happy Friday everyone! We currently have a Ventana Ultra. I am wondering what 
everyone is doing/running for a negative reagent control and/or a negative 
tissue control?
Thanks in advance!
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
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[Histonet] AFB contaminant

[Abbott, Tanya]

Has anyone ever had a problem with a possible contaminant in their AFB hand 
stain? If so, how did you deal with it? Any suggestions?
Ours actually looked like an AFB like organism, which we later determined to be 
negative.
Thanks!

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] Subject: high school donations

[Abbott, Tanya]

HI all,
I've had the pleasure of meeting Adelle and two of our students! She has a 
wonderful program; her students are knowledgeable and very enthusiastic. Please 
help her out wherever you can!
Tanya Abbott 
St Joseph Medical Center
Reading PA

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Wednesday, June 17, 2015 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 139, Issue 17

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit

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Contents of Histonet digest...


Today's Topics:

   1. (no subject) (sris...@mail.holyname.org)
   2. Myogenin (Vanessa Keeton)
   3. high school donations (Schade, Adelle)
   4. Decontamination of Ventana Ultra (Victoria Baker)
   5. Fairbanks, Alaska job opening (Travel Histotech HTL)
   6. Bond Max Available (Richard Yeo)


--

Message: 1
Date: Tue, 16 Jun 2015 14:16:30 -0400
From: sris...@mail.holyname.org
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Message-ID:
ofc9cb199c.32e69070-on85257e66.00642e2f-85257e66.00646...@holyname.org

Content-Type: text/plain; charset=US-ASCII

Hi All,

Does anyone know where we could get the original grey antibody storage racks 
used to store antibodies in the refrigerator?  We are using ventana antibodies. 
Thanks in advance.

Mala









Holy Name Medical Center is ranked among the top hospitals in the nation for 
patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If you 
are not the intended recipient, you are hereby notified that any disclosure, 
copying, distribution, or taking of any action in reliance on the content of 
this message is strictly prohibited. If you have received this communication in 
error, please notify the sender by replying to this message, and then delete it 
from your system.






--

Message: 2
Date: Tue, 16 Jun 2015 15:40:32 -0400
From: Vanessa Keeton vanessa.keeto...@gmail.com
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Myogenin
Message-ID:
CAD5hts_-a=v_kgxhf6ghzp9oqfjgodjpcoi-lgbqgfst93a...@mail.gmail.com
Content-Type: text/plain; charset=UTF-8

I am looking for information on Myogenin IHC stain such as origin/history, 
uses, and any other pertinent information for a research paper for college.

Thank you in advance.


--

Message: 3
Date: Tue, 16 Jun 2015 20:33:19 +
From: Schade, Adelle a_sch...@conradweiser.org
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] high school donations
Message-ID:
e708b96c127027449ab2337747f80b5f7e3e7...@cwmail.cwasd.net
Content-Type: text/plain; charset=iso-8859-1

Hello,
I am posting to ask for any information or direction this group may be able to 
give (or leads for contacts) concerning the possibility of donations to a high 
school science/ histology laboratory.

I am a high school teacher (Conrad Weiser HS, Robesonia, PA) and Cell/ 
Molecular Biology PhD student (Univ. of the Sciences, Philadelphia, PA).  I 
developed a histology laboratory this year in my high school and it has taken 
off!  The students are extremely interested in learning and experimenting 
utilizing histological technique.  I am holding a summer program opening the 
lab for students to work.  Initially I thought I would have 5 to 10 students 
that would want to work over the summer; 28 students are attending.  It is 
inspiring to see such enthusiasm!  With this in mind, I would like to try to 
accumulate some additional equipment/ supplies.  If you know of any 
laboratories that are closing/ shutting down/ or equipment/supplies that are 
not being utilized and there is a possibility of donating these items to a 
public high school please email me off list 
(a_sch...@conradweiser.orgmailto:a_sch...@conradweiser.org).  We are looking 
for any histology related equipment and supplies (
 especially a tissue processor- we are manually processing at this point); as 
well as any basic laboratory equipment/ 

[Histonet] Pipettes

[Abbott, Tanya]

CAP checklist ANP.23085 refers to Pipettes that are used for quantitative 
dispensing of material are checked for accuracy and reproducibility at least 
annually, and results documented.
We perform our Special Stains by hand. So we use disposable, glass, Non Class A 
pipettes. About 15 in a bag. We obviously do not check these one by one for 
reproducibility. I did call CAP and although he tried to be helpful, I still am 
confused!  Any suggestions on how anyone else handles this?
Thanks in advance,
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] ANP.22760 New Reagent Lot Confirmation of Acceptability

[Abbott, Tanya]

Can you tell it's review the CAP checklist time?!! In regards to ANP.22760, 
New Reagent Lot Confirmation of Acceptability, how does everyone handle it? I 
have a procedure in place, but I would like it to flow a bit better.
Thanks in advance! Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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RE: [Histonet] FW: Release of blocks to research facilities

[Abbott, Tanya]

Luckily we do have a research coordinator who takes care of all of these for us 
and he is very thorough. He obtains consent and deals with all costs. I am
thinking that I just need to verify that the facility receiving the block (s) 
are retaining them for the appropriate amount of time, which they are.
But yes, all good food for thought! Thanks for all the input folks!
Tanya

-Original Message-
From: James Watson [mailto:jwat...@gnf.org] 
Sent: Tuesday, March 17, 2015 12:18 PM
To: 'WILLIAM DESALVO'; Abbott, Tanya
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] FW: Release of blocks to research facilities

Bill is correct, CAP is actually the least of your worries.  There are many 
regulations about human tissue used in research (I do not know them all), but 
patient consent is essential and in some cases the patient must actually agree 
to the specific research project.  Here we have regulations about control block 
tissue that we use, we cannot get tissue directly from a local hospital.   Also 
the laws are different in each country.  There is a lot of bioethics involved.

https://urldefense.proofpoint.com/v2/url?u=http-3A__bioethics.od.nih.gov_humantissue.htmld=AwIFAwc=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmawm=OCnLOvVFGUusxYsBbq-Ykuqe0CPoX1ZNX9HWkbBcgXEs=RlbMe-0hfDNzmnp9qRfqYfeoqOM6X3Mg6YcKEkC3vl0e=
 

Human Tissue Ownership and Use in Research: What Laboratorians and Researchers 
Should Know 
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.clinchem.org_content_56_11_1675.full.pdfd=AwIFAwc=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmawm=OCnLOvVFGUusxYsBbq-Ykuqe0CPoX1ZNX9HWkbBcgXEs=cyiM7Uax7JX_cIRMQBNYnrjVrhBa-8Aw3ekWJvXuuA0e=
 




James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Tuesday, March 17, 2015 8:45 AM
To: Abbott, Tanya
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] FW: Release of blocks to research facilities

There are other issues, besides CAP, that you should consider when releasing 
blocks.
1. A block is a medical record. Has the patient consented to release for 
research?
2. If the block is released prior to 14 days discharge and there are any 
charges, the charge must be sent to your hospital. Post 14 days the charges are 
sent to insurance and patient.
3. Will the research lead to a commercial product? Again, written patient 
consent.
4. You may be able to charge the research facility for sending the block. 
Administrative costs.
5. Communicate with patient is a must.
6. Get written consent and have the receiving facility verify they will handle 
properly, retain or return the block d you will get copies of data for patient 
file.

Make sure you reduce your risk.

Sent from my iPhone

 On Mar 17, 2015, at 5:38 AM, Abbott, Tanya tanyaabb...@catholichealth.net 
 wrote:
 
 I did call the CAP and they stated first of all, that it is a very confusing 
 checklist component! But I was told that the research facility is taking on 
 the ownership of the block, and we need to ensure that whoever we are sending 
 our blocks to are following the proper retention protocols.
 
 From: Abbott, Tanya
 Sent: Monday, March 16, 2015 3:33 PM
 To: 'histonet@lists.utsouthwestern.edu'
 Subject: Release of blocks to research facilities
 
 CAP checklist ANP.12500 refers to Record Retention. I am looking 
 specifically at NOTE 2: Regarding extra-institutional release of blocks for 
 research purposes.
 I am wondering how everyone handles this, especially if you have only 1 block 
 on newly diagnosed patient and the Doctor wants it sent out for research.
 Thanks in advance for your help! Tanya
 
 Tanya G. Abbott
 Manager Technologist
 Histology/Cytology
 St Joseph Medical Center
 (phone) 610-378-2635
 
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[Histonet] FW: Release of blocks to research facilities

[Abbott, Tanya]

I did call the CAP and they stated first of all, that it is a very confusing 
checklist component! But I was told that the research facility is taking on the 
ownership of the block, and we need to ensure that whoever we are sending our 
blocks to are following the proper retention protocols.

From: Abbott, Tanya
Sent: Monday, March 16, 2015 3:33 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Release of blocks to research facilities

CAP checklist ANP.12500 refers to Record Retention. I am looking specifically 
at NOTE 2: Regarding extra-institutional release of blocks for research 
purposes.
I am wondering how everyone handles this, especially if you have only 1 block 
on newly diagnosed patient and the Doctor wants it sent out for research.
Thanks in advance for your help! Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] Release of blocks to research facilities

[Abbott, Tanya]

CAP checklist ANP.12500 refers to Record Retention. I am looking specifically 
at NOTE 2: Regarding extra-institutional release of blocks for research 
purposes.
I am wondering how everyone handles this, especially if you have only 1 block 
on newly diagnosed patient and the Doctor wants it sent out for research.
Thanks in advance for your help! Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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privileged. If you are not the intended recipient, notify the sender at once 
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[Histonet] Body storage refrigerator temperatures

[Abbott, Tanya]

Does anyone else have a problem getting their body storage refrigerators to 
maintain the 1.1°C to 4.4°C temps to meet CAP guidelines?
Thanks in advance for your responses!
Tanya


Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] RE: Histonet Digest, Vol 133, Issue 21

[Abbott, Tanya]

I have been in my new role as a Pathology manager for a little over a year now 
and I don't know what I would have done without Histonet! It is a fabulous 
resource, and everyone's' silliness and lightheartedness makes me laugh!! Thank 
you everyone for all of your help, I'll continue to pick your brains in the new 
year!!
Happy Holidays!!!

Tanya G. Abbott
Histology/Cytology
St. Joseph Medical Center
610-378-2635

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Saturday, December 20, 2014 1:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 133, Issue 21

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Today's Topics:

   1. Histonet anniversary and welcome to Dr Sengupta  (Linda Margraf)
   2. Thank you Dr Margraf! (mtitf...@aol.com)
   3. RE: Histonet anniversary and welcome to Dr Sengupta
  (Weems, Joyce K.)
   4. RE: Histonet anniversary and welcome to Dr Sengupta 
  (Marcum, Pamela A)
   5. RE: Histonet anniversary and welcome to Dr Sengupta
  (Whitaker, Bonnie)
   6. RE: Histonet anniversary and welcome to Dr Sengupta
  (Douglas Porter)
   7. Re: Histonet anniversary and welcome to Dr Sengupta 
  (Michael Ann Jones)
   8. unsubscibe (Fischer, Dolores)
   9. unsubscribe (Fischer, Dolores)
  10. Request Recommendation for Fixative/Stains Article
  (Ryan Michael Hickey)


--

Message: 1
Date: Fri, 19 Dec 2014 13:49:17 -0600
From: Linda Margraf lindamarg...@gmail.com
Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID: bbeab515-86d7-4259-a3eb-944bc4bf8...@gmail.com
Content-Type: text/plain;   charset=us-ascii

 Dear Histonetters,
 In a couple of weeks, Histonet will celebrate its 19th anniversary! It is 
 hard to believe it has been in existence that long. I would like to say 
 thanks to all the members who contribute to the list, sharing their time and 
 expertise with others in histology-related fields. We currently have 3800 
 subscribers from at least 30 countries ( last time I counted).  It is amazing 
 to see how the list continues to provide new insights and information after 
 all these years.
 I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr 
 Sandy Cope as a co-administrator of the list with me. Anita is also a 
 pediatric pathologist and she will help with some of the issues with list 
 subscribing etc. Please continue to be patient with us if you are having list 
 issues as we often get busy in our day jobs. If your message to the list 
 doesn't post and it says it is because you are not a member, please see if 
 you need to update your email address on the membership page. Remember you 
 can only post to the list from an email address that matches exactly with one 
 on the subscriber list. You can always send us an email at the 
 histonet-owners address and we will try to help you out. Best wishes for a 
 happy and safe holiday season.
 Linda M
 Histonet administrator
 
 Sent from my iPhone


--

Message: 2
Date: Fri, 19 Dec 2014 15:15:50 -0500
From: mtitf...@aol.com
Subject: [Histonet] Thank you Dr Margraf!
To: histonet@lists.utsouthwestern.edu
Message-ID: 8d1e9dfb772dc91-b24-62...@webmail-va031.sysops.aol.com
Content-Type: text/plain; charset=us-ascii

Thank you Dr Margraf, Dr Cope and now Dr Sengupta for giving up your time and 
hosting the Histonet. It is an ideal way for histotechnologists and others to 
be kept informed.


And Merry Christmas to everyone from the heart of Dixie!


Michael Titford
Pathology
USA Mobile
AL  USA


--

Message: 3
Date: Fri, 19 Dec 2014 20:21:43 +
From: Weems, Joyce K. joyce.we...@emoryhealthcare.org
Subject: RE: [Histonet] Histonet anniversary and welcome to Dr
Sengupta
To: 'Linda Margraf' lindamarg...@gmail.com,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:

e3a4ebd57a691646bcced4aa5911a030011c401...@e14mbx12n.enterprise.emory.net

[Histonet] Pennsylvania Department of Health permit

[Abbott, Tanya]

This is for you Histoneters In Pennsylvania! Are you all familiar with PA 
Department of Health Act 122-2013? I am wondering how the Pennsylvanians are 
interpreting the rules for sending out slides for consults to other states? Are 
you expecting those consulting labs to have PA DOH permits? Some of our 
consulting labs perform further testing that we cannot perform here in our lab, 
and some perform further testing that we can perform here, to aid in their 
consult opinion. And sometimes they just look at our material and give an 
opinion. Any ideas?
Thanks in advance and happy holidays!! Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Water baths

[Abbott, Tanya]

Does anyone take daily temperature checks of their water bath (used for cutting 
only) for CAP?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Competency Assessment

[Abbott, Tanya]

Does any have their Surgical Secretaries perform any sort of annual competency? 
If so, any ideas you could share please? Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Processor

[Abbott, Tanya]

Looking for a tissue processor to process 200-300 cassettes per run, any 
favourites?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Recycler

[Abbott, Tanya]

We have a CBG Biotech recycler. We recycle Americlear, xylene substitute. How 
does everyone dispose of the waste alcohol and waste paraffin?
Thanks and have a great weekend!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Fixation with phenol formalin

[Abbott, Tanya]

We have a brain fixing with phenol formalin, any suggestions on anything 
special we need to do with the tissue before processing?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Storing specimens

[Abbott, Tanya]

How does everyone handle storage of specimens for 2 weeks post sign out? We are 
a small lab (as is everyone on Histonet, I'm sure!!) and trying to figure out 
the best way to organize to make the flow go smoothly. Meaning, we are hoping 
not to have to check each specimen to determine it was signed out before we 
discard. Any suggestions?
Thanks! Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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immediately reply to the sender and delete the message completely from your 
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[Histonet] RE: Histonet Digest, Vol 131, Issue 22 ] Reticle Stain

[Abbott, Tanya]

I agree with Rebecca. We had this problems constantly at the previous hospital 
I worked at and were in contact with Ventana frequently regarding this issue. 
It is my understanding that this hospital is still having problems and that a 
Ventana rep is in there frequently to perform decons. It's all about the 
decons! Very frustrating! Where I am now, we hand stain our retics, no 
problems!! Good luck!

Tanya G. Abbott
Histology/Cytology
St. Joseph Medical Center
610-378-2635


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, October 20, 2014 1:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 131, Issue 22

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Today's Topics:

   1. Staining bacteria in cryo + paraffin (Mikael Niku)
   2. Dallas job openings (Michelle Lamphere)
   3. Protargol S (Freida Carson)
   4. Re: Protargol S (Tina Van Meter)
   5. RE: Histonet Digest, Vol 131, Issue 20 (Riesen, Rebecca)
   6. Mount-Quick for tissue transfer? (Eva Permaul)
   7. Re: Protargol S (Rene J Buesa)


--

Message: 1
Date: Mon, 20 Oct 2014 14:00:11 +0300
From: Mikael Niku mikael.n...@helsinki.fi
Subject: [Histonet] Staining bacteria in cryo + paraffin
To: histonet@lists.utsouthwestern.edu
Message-ID: 5444eb3b.5050...@helsinki.fi
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hello!

What is your preferred method to show ANY bacteria (mostly intestinal
flora) on cryo AND paraffin sections? Two different stains OK.

With best regards,
Mikael



--

Message: 2
Date: Mon, 20 Oct 2014 13:08:35 +
From: Michelle Lamphere michelle.lamph...@childrens.com
Subject: [Histonet] Dallas job openings
To: 'histonet@lists.utsouthwestern.edu'
histonet@lists.utsouthwestern.edu
Message-ID:
eee916a21070714f918c63c127e620a33b617...@cmcpbexmail03.childrens.med
Content-Type: text/plain; charset=us-ascii

We have full and part time openings at Children's Health in Dallas, TX.  
Positions require qualification for High Complexity Testing.  Please contact me 
directly to inquire.

Michelle Lamphere
Senior Tech, Histology
Children's Health
214-456-2318
michelle.lamph...@childrens.com

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you are not the intended recipient, any disclosure, copying, printing, or use 
of this information is strictly prohibited and possibly a violation of federal 
or state law and regulations. If you have received this information in error, 
please notify Children's Medical Center Dallas immediately at 214-456- or 
via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its 
affiliates hereby claim all applicable privileges related to this information.


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is intended only for the use of the
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or state law and regulations. If you have received this information in error, 
please notify Children's Medical Center Dallas immediately at 214-456- or 
via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its 
affiliates hereby claim all applicable privileges related to this information.



--

Message: 3
Date: Mon, 20 Oct 2014 08:43:00 -0500
From: 

[Histonet] Ebola

[Abbott, Tanya]

Dare I ask?! Are any Pathology labs discussing what to do with 
specimens/precautions, etc. regarding a person with a potential Ebola infection?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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immediately reply to the sender and delete the message completely from your 
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[Histonet] More info on EBUS

[Abbott, Tanya]

Sorry I was a little vague on my quest for answers!! It was our first 
one,!
Pathologist was down in the Lab (we are a small hospital) and slides were sent 
down. He gave a dx/adequacy (none of them were really adequate for a proper dx) 
for each slide, they sampled 3 levels multiple times, some passes were grouped 
together. Slides came down about 7 different times for a total of 22. We 
received slides that some appeared to be dropped on and some were smears. And 
then after the procedure received aspirates in Cytolyte.
It was a very interesting day!
Much thanks!  Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net




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[Histonet] Rapid on site evaluation for EBUS

[Abbott, Tanya]

This is possibly more of a Cytology question, but we are a small Path lab that 
covers it all!! We just did our first EBUS, and are wondering how to code for 
the Rapid on site evaluations, any suggestions?
Thanks! Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Gram stain

[Abbott, Tanya]

We only perform a few Gram stains a year, hardly seems worth It to keep a kit 
and expire reagents! Does anyone have an idea for other options? Ie, doing the 
stains in Micro? Checking with their
BD rep to see if we can do their Gram stain kit on formalin fixed tissue,...

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Back up Tissue processor

[Abbott, Tanya]

Does anyone keep a back up tissue processor in your lab? And if so, what sort 
of requirements are there for CAP for insuring it is working properly, etc?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] VIP 1000

[Abbott, Tanya]

To go along with my last question, does anyone know where I could get charcoal 
filters for the VIP 1000 tissue processor? I have a letter from 2002 from 
Sakura saying parts for this will
probably only be available for 2 more years, so that's only 10 years 
ago,...!! Assuming if I can't get filters, I can't run it anyway?!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Clearium?

[Abbott, Tanya]

This is a bit more of a Cytology question, but I thought I would survey my 
Histo friends! We are starting to have bubbles with our PAP slides, and it 
actually seems to occur after drying, initially after coverslipping (by hand) 
there appears to be no bubbles. We use Clearium, so we can go directly from 
alcohol. I am a new manager, and this lab is very adversed to Xylene.  Any 
ideas?
Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Acid Cleaned glassware

[Abbott, Tanya]

We just acquired a brand new laboratory microwave to replace ours from the 
1980s,..so we are optimizing our GMS stain. Currently we would let the 
glassware sit in
Chromic acid for 1 hr to chemically clean the glassware before staining. Now we 
are using the CAP required polypropylene vented microwave containers. Can we 
skip the chromic
Acid step?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Tissue Tek Prisma coverslipper

[Abbott, Tanya]

Does anyone who uses the Tissue Tek Prisma coverslipper have an external 
ventilation source? Or is the internal filtering system the instrument uses 
good enough?
Thanks!
Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Combo xylene/xylene substitute

[Abbott, Tanya]

We use xylene substitute for everything in our lab instead of xylene, including 
in our Prisma stainer. We are looking to purchase the coverslipper and link, 
which needs to use xylene with the coverslipper tape, and also in the last 
station of the stainer. Has anyone ever had to use both xylene and xylene 
substitute (Citra-clear) in their staining process?  Any suggestions?
Thanks!
Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] New Microwave

[Abbott, Tanya]

We have finally taken the plunge (thanks to our CAP inspection!) to a new 
beautiful stainless steel vented microwave to replace our kitchen one from the 
80s!
Any suggestions on how to meet all the CAP guidelines for this? We only use the 
microwave for Special Stains, not for processing.  Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] ANP.22970

[Abbott, Tanya]

Per CAP checklist ANP.22970, it lists the expected published benchmarks for 
ER/PR, does anyone know where to find them for Her2 or MMR?
Thanks and Happy Friday!!
Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] H. pylori

[Abbott, Tanya]

Can anyone recommend a good commercially bought slide for H.pylori?
Thanks!  Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Vented containers for microwave

[Abbott, Tanya]

What sort of vented containers is everyone using for microwave Special stains?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Antibody validation

[Abbott, Tanya]

I am wondering what process labs are doing to validate new antibodies per CAP 
guidelines?
Thanks!
Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Gross only

[Abbott, Tanya]

How does everyone bill for Gross Only?
Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] pH sticks

[Abbott, Tanya]

Is anyone aware if there are pH indicator strips that read to the 0.1?
Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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named addressee(s) and contain confidential information. If you are not an 
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[Histonet] Billing

[Abbott, Tanya]

Does anyone bill for a technical component for send outs? Meaning, billing 
for supplies, time it takes to process sendout/receive them back in, etc?
Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Validating New IHC antibodies

[Abbott, Tanya]

What's everyone's go to # for Pos/Neg slides when validating new IHC antibodies?
(Not counting Her2 and ER/PR, of course!)
Thanks!
Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Negative Controls for IHC

[Abbott, Tanya]

I just read in Advance Dec 2013 that there is a possibility of laboratories 
utilizing fewer QC controls
to cut costs? Has anyone stopped running negative controls for IHC?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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named addressee(s) and contain confidential information. If you are not an 
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[Histonet] SNOMED

[Abbott, Tanya]

Can anyone give me a brief synopsis of how this SNOMED thing works? Please?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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named addressee(s) and contain confidential information. If you are not an 
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[Histonet] Cryostat Decontamination

[Abbott, Tanya]

I am looking for a procedure and product to use for decontamination after a 
potential TB case, help please!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Cryostat

[Abbott, Tanya]

Need to decon my cryostat

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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