Re: [Histonet] Ventana temp veriifer slides
I'm doing the even one quarter and the odds the next also. I number the slides in Sharpie to cprrespond to the heat pad # after they are finished and take a photo of them for documentation, then toss the slides. es...I've decided to do the even's 1 quarter and the odds the next quarter (because of cost)...do you just run them, document it and throw them away? Or does anyone make a copy of them and save in the QC book? Thanks! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Negative Reagent Control
Our Prayers have been answered!!! Richard Cartun rcar...@harthosp.org 7/17/2012 7:04 PM Most of you know that I have been advocating for the elimination of the Negative Reagent Control when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence: Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director. I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to loud applause. This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica CV5030 coverslipper issues
If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to use as a coverslipper. histot...@imagesbyhopper.com histot...@imagesbyhopper.com 5/21/2012 3:30 PM We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken ears on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, Horn, Hazel V hor...@archildrens.org wrote: We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original
RE: [Histonet] FW: Redneck Lent
I'm a redneck Catholic too and I heard it weeks ago and thought it was pretty funny. Still doesn't belong on a public forum though. Britton, Josette C jcbrit...@cheshire-med.com 3/27/2012 3:16 PM I am a redneck and a catholic and was not offended at all! As a matter of fact it made me hungry! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Tuesday, March 27, 2012 11:44 AM To: Davide Costanzo Cc: Histonet Server Subject: RE: [Histonet] FW: Redneck Lent Hey how bout us rednecks? This redneck wasn't at all offended. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Tuesday, March 27, 2012 10:28 AM To: Davide Costanzo Cc: Histonet Server; JOSEPH FRAZEE; LINDA FRAZEE; mike tony siltman; Taylors Cars Subject: RE: [Histonet] FW: Redneck Lent Your last sentence was inappropriate. Ye who live in glass houses should not cast stones. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 From: Davide Costanzo [pathloc...@gmail.com] Sent: Tuesday, March 27, 2012 11:22 AM To: Boyd, Debbie M Cc: JOSEPH FRAZEE; Histonet Server; Taylors Cars; LINDA FRAZEE; mike tony siltman Subject: Re: [Histonet] FW: Redneck Lent I, personally, found the joke very funny. I find a lot of distasteful jokes funny - I actually prefer them over anything clean. That does not take away the fact that discussing religion, or politics (with humor or in any other form) has no place in the workplace. Histonet is, in many ways, an extension of the workplace. I also do not discuss religion or politics with strangers, and there certainly are more strangers that read this blog than folks we know. While I was not personally offended by that joke, it is very conceivable to think that some folks would be offended. As I told one replier - had this joke been about Jews it would have been something folks reacted to harshly. And, for good reason. So we cannot joke about Jews or Muslims, but Catholics are fine? I respectfully disagree - ALL religions and posts of humor in reference to a religion on a public listserv is a terrible idea. And, incidentally, this support for those that could be offended is coming from me - a person that thinks ALL religion is a joke in the first place. On Tue, Mar 27, 2012 at 8:08 AM, Boyd, Debbie M dkb...@chs.netmailto:dkb...@chs.net wrote: For goodness sakes! It is a joke. First of all it was accidently sent to HistoNet per Joseph's second email. But most of all can't we just loosen up a bit and laugh at/with each other? Every religion, race, gender, etc. has had jokes made about it. Give the guy a break. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050tel:804-765-5050 l FAX 804-765-8852tel:804-765-8852 From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-bounces@lists. utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edumailto:histonet-bounces@lists .utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfra...@hotmail.commailto:jfra...@hotmail.com] Sent: Monday, March 26, 2012 6:48 PM To: Histonet Server; Taylors Cars; LINDA FRAZEE; mike tony siltman Subject: [Histonet] FW: Redneck Lent Date: Mon, 26 Mar 2012 19:55:27 +0100 From: spoeri...@yahoo.commailto:spoeri...@yahoo.com Subject: Fw: Fwd: Redneck Lent To: karen.green...@hobbylobby.commailto:karen.green...@hobbylobby.com; stewartdap...@hotmail.commailto:stewartdap...@hotmail.com; yvette.fette...@basf.commailto:yvette.fette...@basf.com; footch...@yahoo.commailto:footch...@yahoo.com; frazeeli...@hotmail.commailto:frazeeli...@hotmail.com; jfra...@hotmail.commailto:jfra...@hotmail.com; donna.lu...@gmail.commailto:donna.lu...@gmail.com Kerri - Forwarded Message - From: Sharen Pray praysha...@yahoo.commailto:praysha...@yahoo.com To: Ruth Posey ruthalpo...@yahoo.commailto:ruthalpo...@yahoo.com; LueAnn Root lar...@ymail.commailto:lar...@ymail.com; Marjorie Norris nursenor...@yahoo.commailto:nursenor...@yahoo.com; Tom Voss, Sr. tomv...@wildblue.netmailto:tomv...@wildblue.net; Taber Stewart texcon.ta...@gmail.commailto:texcon.ta...@gmail.com; MONTIE L WINTERS molo...@yahoo.commailto:molo...@yahoo.com; Terry Maloney maloney_te...@yahoo.commailto:maloney_te...@yahoo.com; kerri spoering spoeri...@yahoo.commailto:spoeri...@yahoo.com; Kenny Debbie Hager kanddha...@att.netmailto:kanddha...@att.net Sent: Saturday, 24 March 2012, 21:06 Subject: Fw: Fwd: Redneck Lent Each Friday night after work, Bubba would fire up
Re: [Histonet] Smad4 (B-8) / DPC4
I've tried to work it up on the XT also with inconsistent, weak results. If you get a reply on this one, let me know. Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 Giroux, Stacy stacy.gir...@stjohn.org 3/6/2012 7:35 AM Hi, I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are currently trying to work up this antibody for validation; however, we are having significant problems getting it to work consistently. We are using the Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology. If anyone has protocol information to share regardless of platform used it would be greatly appreciated. Thanks, Stacy Stacy Giroux, HTL(ASCP) Operations Coordinator, Histology St. John Hospital Medical Center Phone: 313-343-3130 Fax: 313-343-4965 Histotechnology Professionals Day - March 10, 2012 CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC + control question
My vote has always been for vimentin. See Dako's booklet IHC Staining Methods, 5th Ed, Chapter 19 addresses this. Angie Chiriboga, Luis luis.chirib...@nyumc.org 3/6/2012 9:01 AM Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested
I've had a temp, who we interviewed over the phone, come in and sit down at a microtome and create the most horrendous slides I've ever seen. He lasted a week and we sent him back from whence he came. I don't think he was EVER a Histotech or if he was it was many, many moons ago. Point is.he snowed us all during the interview. Just thought I'd throw that out there. Kim Donadio one_angel_sec...@yahoo.com 1/30/2012 10:01 PM Oh come on. The truth of the matter of why I like to give a manual test to new hires is because people are graduating some Internet programs without the technical skills to function in a lab. Not all. But I've seen a lot. Just saying:) I don't think it should be made a big deal. You take a drivers test to drive. Peoples lives are on the line in each case. Does that a lone mean I don't hire them. Probaly not. I just need to know how much personal investment of my time I am going to need to give .. Runs for her pillow of dreams :). Nite nite Kim Sent from my iPhone On Jan 28, 2012, at 4:25 PM, Thomas Jasper tjas...@copc.net wrote: Ray, Took the time to read your post. You make excellent points. Getting at the gist of your wannabee comments. What boggles my mind is - how or why someone would try to pull something off like that. Sooner or later (hopefully sooner...like before actually hiring them) the charade will be discovered. Misrepresenting oneself and false or misleading information given on an application is generally grounds for dismissal. Seems to me this isn't Leonardo di Caprio and Catch Me If You Can. In the end you are right about finding ways to determine if an applicant is legit. I've come to believe that in the Histology world - if you meet or hear of someone you don't know...someone you do know...knows them. At least that seems to be true almost all the time. Kind regards, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of koelli...@comcast.net Sent: Saturday, January 28, 2012 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] interview cutting-OT-disarmingly long for deletiondisinterested Or as Gayle wisely pointed out it might be interview sectioning to differentiate those who cut out on an interview. While there is no right or wrong to this question, I'm still not convinced that it is a useful tool for you or HR to just have a routine can cut (section) on rotary microtome check box on application the same as you do for a current address or reference contact check box on a form. As I pointed out in my original stupid reply, willfully breaking my own internal rule to avoid taking up these gray (not black and white scientific) discussions, it would depend on the circumstance (unknown person from unknown parts vs. someone from part of the histology community well known). If I call x who I've known for years about an applicant y who is applying and worked with x and am told Oh! y worked for us for last 4 years. He/she along with z and zz were our 3 who sectioned (#) blocks a day. Devastated to see him/her go but know they had to move along with husband/wife. Great cutter and everyone liked him/her. Having him/her sit down to now cut 10 blocks to see if they can cut as a routine question accomplishes WHAT? If someone mysterious with no background walked in, sure have them cut although there have been numerous fantastic options already posted how to weed them out prior to sectioning a finger off. A (purposely) mis-processed block with tissue now shrunken in from block face and a question of we need a recut, what would you do for this block will let you know in about 2 seconds whether or not this is a histotech impostor. Or looking at a blandly stained, necrotic section under microscope and asking interpret this section will tell you something of who or what this person is. Personally, I'd far rather have a person who is energetic, scientifically and intellectually confident and talented, personable, works well within the symphony of histology and cuts 8 blocks and leaves a few wrinkles in this new environment set-up than a (female or male) diva who cuts 10 perfect blocks but who has that nearly imperceptible tint of not a complete team player or dubious personality. A routine check box can cut I think is just a waste of time and resources unless a particular circumstance warrants it. Someone asked would you hire a secretary without a wpm typing test. Absolutely, beyond any doubt. If the transcriptionist next door wants a secretary position and routinely types 3 times faster than is required as a secretary; why a wpm test? If I call someone I know across state where this applicant
Re: [Histonet] RE: time off
I've witnessed that granting time by seniority lets you open to abuse. I know of an employee with high seniority who takes the whole week after Christmas every year. Our policy allows only one person off per day per shift. So then no one else ever can take off at Christmastime. Her peers complain to management about it, but they won't say anything to her. Just my two cents. Andrea abeharry...@gmail.com 1/3/2012 5:40 PM We also have a policy on the number of people that can be off during peak times. Our vacation schedule runs from June to June of the following year. Staff have up until a deadline of January 31 to put in their request for the next vacation year, whether it is one day or weeks. The requests handed in by this time period are granted based on seniority. ( in our institution they figure it's one of the only perks to being a senior!) After the January 31 deadline it is first come first serve. All vacation requests must be submitted on a vacation request form and time stamped when handed in. This way if two people ask for the same day the person who handed it in with an earlier time stamp is granted the time off. All of this is written in our scheduling guidelines. It seems to work pretty well. On 2012-01-03, at 4:32 PM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: We have a Laboratory policy which states that holidays will be rotated. There is also a section which gives a limit to the amount of time an employee can have off during peak vacation time. For example, our staff can only have a maximum of two weeks off during the peak summer time, and no more that 2 days off during the last two weeks of December. I personally have no problem with staff requesting time off early in the year, but I do ask that they discuss with their coworkers before giving me the request. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 03, 2012 4:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet
Re: [Histonet] IHC on Bones
pray. McCabe, Sara J. sjmcc...@drmc.org 12/30/2011 1:02 PM Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CK15 with Ultraview
Is anyone running Cytokeratin 15 on the Ventana Benchmark XT or Ultra platform?? Would you be so kind as to share the protocol?? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] c-myc
Does anyone send slides out for c-myc staining by IHC? Where do you send to? Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] saving slides between levels for IHC?
we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, liver core bxs, prostate needle core bxs. Harrison, Sandra C. sandra.harris...@va.gov 10/25/2011 1:59 PM Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cat Scratch fever
I was just watching his show last night! That's entertainment! Galbraith, Joe joseph-galbra...@uiowa.edu 10/12/2011 10:19 AM No one under 30 would have a clue what that joke means. Lol Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, October 12, 2011 8:38 AM To: Andrew Byrnes; Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cat Scratch fever Too true! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Byrnes Sent: Wednesday, October 12, 2011 8:31 AM To: Mauger, Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cat Scratch fever Good song! Andrew Byrnes AccelPath.com On Oct 12, 2011, at 7:35 AM, Mauger, Joanne mau...@email.chop.edu wrote: Newcomer Supply sells cat scratch control slides. Jo -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay Sent: Wednesday, October 12, 2011 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cat Scratch fever Does anyone know where I can get a paraffin control block for cat scratch fever? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I too spoke to one vendor who claimed that the floaters are not an issue and that their bottles drew below the level of the floating floaters. I didn't feel insulted, but did feel that this salesperson obviously had no actual lab experience or they never would have made such a ridiculous claim. I always put the opinions and experiences of other Histotechs far ahead of anything I hear from sales reps. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 Janice Mahoney mamaw...@hotmail.com 10/5/2011 8:11 PM Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding floaters and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of HE stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that All Histo techs know that floaters come from the water bath. Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the floater is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] STAIN PRECIPITATE
We've been seeing high background staining on abt 10% of our HPs as well. We use Cellmarques predilute on the XT as well. Been happening for a couple months. Sara Baldwin/mhhcc.org sbald...@mhhcc.org 9/23/2011 3:57 PM Hi Histonetters I have the Ventana xt, H pylori from cell marque and my pathologists says there is too much stain precipitate and was wondering if I could do something about it my protocol is mild cc1 standard ABY H pylori 32 min ultra wash and counterstains Any help is appreciated Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
same here. McMahon, Loralee A loralee_mcma...@urmc.rochester.edu 9/22/2011 1:43 PM That is what we do as well. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreig...@gmail.com] Sent: Thursday, September 22, 2011 1:18 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] new antibody lot We run 1 slide from each new lot, and compare it with the previous stained slide and have it signed off. We have several lot to lot slides which come in very handy for seeing how the antibodies will vary from lot to lot. On Thu, Sep 22, 2011 at 7:47 AM, anita dudley azdud...@hotmail.com wrote: how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody lot
yes, they are recommendations. Unless it has changed, I believe it is up to the discretion of your Doctoral Director. Elizabeth Chlipala l...@premierlab.com 9/22/2011 2:07 PM CAP has recommendations from the following paper Recommendations for Improved Standardization of Immunohistochemistry Neal S. Goldstein, MD, Stephen M. Hewitt, MD, PhD, Clive R. Taylor, MD, DPhil, Hadi Yaziji, MD, David G. Hicks, MD, and Members of Ad-Hoc Committee On Immunohistochemistry Standardization 3 tissues need to be evaluated - one that is strongly positive, one that is weak to moderately positive and one that is negative for the particular antibody. If you put these all on one slide then it would just be one slide. Liz From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley [azdud...@hotmail.com] Sent: Thursday, September 22, 2011 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new antibody lot how are others handling new lot numbers of antibodies when you receive them in the lab? thanks for any input. have a great day!!! anita dudley providence hospital mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] pax2
CC1 standard, 32 min incubation with heat disabled. I use Cellmarques predilute and UltraView DAB detection. Dorothy Glass techman...@yahoo.com 7/7/2011 3:28 PM Does anyone have a working protocol for pax2 on the Ventana Ultra or XT? Can you please share on Histonet? Dorothy Glass Supervisor, SEPA LABS Brunswick,Ga. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Luciferase
ok there is a really BAD religious joke coming soon, isn't there? Rene J Buesa rjbu...@yahoo.com 6/30/2011 12:00 PM You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.com sgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.com sgoe...@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Luciferase
You can, but the retrieval involves a young priest and an old priest. ff McAuliffe mcaul...@umdnj.edu 6/30/2011 12:42 PM It is a devil to stain. Geoff On 6/30/2011 12:13 PM, Angela Bitting wrote: ok there is a really BAD religious joke coming soon, isn't there? Rene J Buesarjbu...@yahoo.com 6/30/2011 12:00 PM You would have to have an antibody to it and I don't know any one exists, but I can be wrong. René J. --- On Thu, 6/30/11, sgoe...@mirnarx.comsgoe...@mirnarx.com wrote: From: sgoe...@mirnarx.comsgoe...@mirnarx.com Subject: [Histonet] Luciferase To: histonet@lists.utsouthwestern.edu Date: Thursday, June 30, 2011, 11:55 AM Can you stain for luciferase in tissue? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Anatomicalalal
Shall we all debate the pronunciation of Periodic acid next ?!?! I've heard it 3 different ways so far in my career. Breeden, Sara sbree...@nmda.nmsu.edu 6/13/2011 3:03 PM I just LOVE it when I'm right - and it happens so rarely! The consensus of ten responders so far is ANATOMIC. This just makes sense to me - we don't call them Diagnostical Pathologists, do we? Nah. Keep those correct answers coming my way - I needed the confirmation! And I have $50 toward the rowboat that'll take me to that island. Can you tell I need the diversion today? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana probes
I have a Pathologist asking me to try running Kappa and Lambda by CISH on bone marrow smears. Does anyone have any experience with trying that? Happy strange Tuesday, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Safety glasses required for embedding
We went round with a Safety Dept. employee when I first started here about gloves for embedding. Couldn't convince him that we didn't need to wear them. Rene J Buesa rjbu...@yahoo.com 5/5/2011 3:46 PM No. Rerné J. From: Cindy DeRiso cindy.der...@yale.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 3:11 PM Subject: Re: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hep B
I'm looking for hepatitis B positive control tissue. Willing to trade if I have tissue that you might want. Contact me off line. Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Probes
Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rantanyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Probes
Sorry folks, in my impaired state of mind I made a boo boo. I need 5-6 ml to do 50 tests. If the dispenses are still 100 ul each. Any thoughts? Angela Bitting akbitt...@geisinger.edu 4/28/2011 11:03 AM Ok. Ventana is on my last nerve with this new probe configuration of theirs. I have a 4 ml vial of EBER Probe here in my lab. I am told that this is good for approx 50 tests. So at 100 ul a dispense, I need to bring the volume up to at least 15 ml (probably 16ml). Not a soul under Ventana's employ will tell me what probe diluent to use, or what else I am supposed to do with it. I'm ready to add vodka. If anyone out there has transitioned to the new EBER probe configuration, can you give a gal a break and contact me? Sorry for the Thursday rantanyway Happy Lab Week everyone!!! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Probes resolution
Thanks to all of you who offered your help. I did have a conference call with Ventana this afternoon and all my questions were answered. I'll share with you all what I know so no one else needs to fret needlessly. I didn't receive 2- 4ml vials of EBER Probe. I only received 1 when I ordered it. When you order the 760-1209, you should get 2 vials of Probe 4 ml each. I will be getting the second vial sent out to me tomorrow. So now I will have enough Probe to fill my ISH prep kit. It was a production/shipping error. If Customer Support could have explained that to me this morning, I would never have been so frustrated. Water under the bridge. I have put Ventana back on my Christmas card list. Also, they cannot give you the recipe but if your recipe doesn't work, a TAS can tell you what to try to fix it. I hope this info is helpful. Angie That's what I know, so thought I'd share. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: [IHCRG] Ventana EBER DNP
Your sales rep should supply these to you. I'm having problems with filling my ISH Probe Kit. I logged in my EBER Probe. I registered the Probe kit and went to fill it and it only lets me pick from my Fillable Reagents list, not the Fillable probes list. Tech support can't figure out why this is happening and said they haven't encountered this problem with any other clients. Anyone else having this issue? Mark Tarango marktara...@gmail.com 4/19/2011 11:08 AM If anyone has the catalog numbers for the probes, that would help. I couldn't get this info from Ventana when I asked. They said we have to ask another lab, even for catalog numbers! Mark On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner sfon...@labpath.com wrote: SAME HERE!!! I would love some help with this. We are going to start doing the EBER with Ventana and have no idea where to start. Thanks in advance for ANY suggestions or help you could give. Sheila, HT (ASCP) KDL Pathology Knoxville, TN From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Tallardy, Betty S Sent: Tuesday, April 19, 2011 10:55 AM To: ih...@googlegroups.com Subject: [IHCRG] Ventana EBER DNP Hi Group, Is anyone stuck in the EBER transition with Ventanas' probe changeover? The new product is supplied in a small vial and of course there are no suggestions about how to use the product. If anyone has some ideas on how to proceed with this, I would really appreciate some suggestions. I hate to waste such a pricey product trying to get something workable. Thanks in advance. Betty Tallardy, HT(ASCP)QIHC Dept. of Pathology Presbyterian Hospital 200 Hawthorne Lane Charlotte, NC 28204 704-384-5490 bstalla...@novanthealth.org _ This message and any included attachments are from NOVANT HEALTH INC. and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. If you believe that any information contained in this message is disparaging or harassing or if you find it objectionable please contact Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to repo...@novanthealth.org. Thank you. -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana ultra
each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Benchmark Ultra question - follow up
Augh! I hate when that happens! Gudrun Lang gu.l...@gmx.at 4/13/2011 10:44 AM Thank you all for your replies. After all I have to admit, that the cause was human failure. We found, that the protocols have been changed and noone did remember. That was after a stopped run, where HIER war clicked off for the repetition. And afterward only short HIER was clicked on again instead of mild HIER. – stupid me! Bye Gudrun _ Von: Lynn Lee [mailto:lynnlee2...@live.com] Gesendet: Mittwoch, 13. April 2011 04:14 An: gu.l...@gmx.at Betreff: FW: [Histonet] Benchmark Ultra question - follow up One last thought- do you filter the new antibody solution you made EVERY time when refilling the prep kit? If not, there could be some particulates in the diluent causing a blockage also. Hope these suggestions help or maybe you have already checked this out. Lynn Lee _ From: lynnlee2...@live.com To: gu.l...@gmx.at Subject: RE: [Histonet] Benchmark Ultra question - follow up Date: Tue, 12 Apr 2011 20:10:43 -0600 It's possible the flip lid on the prep kit dispenser top is not completed closed and seated properly. When the dispense hammer goes down, the pressure may not be the same, every time if this is the case. I always check the tip of the dispenser for air bubbles before putting on the machine for EVERY one whether it is a prefilled Ventana dispenser or my prep kit dispenser. Finally something could be clogged in a line somewhere. I worked in R D for Ventana for almost 4 years and have since used the XT and Ultra in other labs and never had a problem with incorrect amount of reagent being dispensed. I guess there's a first time for anything though. I would call Ventana Customer Support. They are great! Lynn Lee B.S., HT(ASCP) Tucson, AZ From: gu.l...@gmx.at To: histonet@lists.utsouthwestern.edu Date: Tue, 12 Apr 2011 18:23:27 +0200 Subject: [Histonet] Benchmark Ultra question - follow up Today I made a further experiment. I did two titration runs. On one slide I added the working solution by pipette on the other slide I added it by pushing the filled PrepKit. Both stainings came out beautiful. - There has to be something wrong with the dispension-amount. Ventana is informed, I hope they find the culprit. Bye Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 11. April 2011 18:48 An: 'Angela Bitting' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] Benchmark Ultra question Yes, that could be true. Some of the PrepKits go harder than others. Perhaps the pressure of the hammer is not big enough. Sometimes the nozzles are not filled equally. (We press always reagens in the nozzle when we prepare the run.) With the Ventana-System there is an additional dilution, because the working-solution is added to a reaction-buffer film under the LCS. Perhaps it makes a difference if you dispense the solution with the pipett-tip under the LCS or if the drop falls on the surface of the LCS. That could be a matter of the LCS-quality, or buffer-quantity , or . I think I'll become mad! Our pathologists have an uncertain unhappiness with the overall staining results, but most of the antibodies work well. Perhaps the majority isn't very sensitiv to small changes in the system and the quality-difference isn't big enough, but some are easier to kill. Regards, Gudrun _ Von: Angela Bitting [mailto:akbitt...@geisinger.edu] Gesendet: Montag, 11. April 2011 18:06 An: gu.l...@gmx.at Betreff: Re: [Histonet] Benchmark Ultra question When I was trained to do titration runs on the BenchmarkXT and Ultras, I was told to titrate 100ul of antibody. I have been suspecting for some time now that the dispensers DON'T dispense a full 100ul. I haven't taken the time to prove it, but your question may have motivated me. That could explain the weak staining. Gudrun Lang gu.l...@gmx.at 4/11/2011 11:48 AM Hi! I have some issues with our new Ultra. Since a few days antibodies, that work usually well, show up very faint. For example the TTF1 (Novocastra). I ordered a new bottle, made a titration and found, that the old titer 1:50 was again well enough. I filled the old PrepKit - and the result was very weak. Then I thought, the PrepKit itself is the culprit. I changed the PrepKit - and again the result was very weak. At that time the working-solution was only three days old. Has anyone an explanation, why the titration works well and the automated dispension doesn't? Regards Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ IMPORTANT WARNING: The information in this message (and the documents attached to it, if any
RE: [Histonet] Ventana ultra
We load our Ultras with the most commonly used antibodies each morning. So that minimizes the number of landing zones we need to use. Also, (if you run both XTs and Ultras) run your antibodies with the longest protocols on Ultra and that will shorten your XT runs. Setlak, Lisa lset...@childrensmemorial.org 4/13/2011 10:54 AM We like ours as well. It's not as continual as we originally thought to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a landing zone in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; barbara.cr...@lpnt.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Kappa/Lambda ISH on Ventana
Ventana can no longer give out protocols. Theses probes are ASR products and the FDA doesn't allow them to tell you the protocol. They can offer guidance if you have a protocol that doesn't work well, but they can't outright tell you a protocol. Another institution that runs Ventana probes is your best bet. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Sebree Linda A lseb...@uwhealth.org 3/31/2011 7:09 PM You should be able to get protocols directly from Ventana. I tried out their K and L ISH probes and they gave us the protocols. They also supplied us with the protocol we use for EBER ISH. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Wilkes Sent: Thursday, March 31, 2011 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kappa/Lambda ISH on Ventana Hi Histonet! I have been tasked with validating Kappa and Lambda on our Ventana Benchmark XT machines. Does anyone who uses this platform have suggestions as to which Ventana protease works best (1, 2 or 3)? Additionally, any other information concerning the optimization (suggested incubation times, RNA regions etc.) would be greatly appreciated. Thanks in advance. Steven srwil...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] reagents with no expiration dates
Call the vendor. Many times they will give you a date based upon the lot number. Michele Carr michelecar...@yahoo.com 3/18/2011 12:49 PM Hi everyone was curious about how to obtain an expiration date for those reagents that don't come with one. I know this is a cap requirement and we are preparing for our first inspection soon. Any info would be appreciated. Thank you all, Michele Carr HTL ASCP Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] S100 A1
I'm looking for a mouse anti human S100 A1 antibody for IHC. My vendor doesn't carry it anymore. Anyone have one that they are using on FFPE tissue? Could you send me ordering info or a copy of your data sheet? Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?
you all saw the little wink ;) at the end of his paragraph, right Shirley A. Powell powell...@mercer.edu 3/1/2011 3:01 PM Bet you he already does. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, March 01, 2011 3:01 PM To: 'Marcum, Pamela A'; 'Jay Lundgren'; Mahoney, Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob at his door! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 3:00 PM To: 'Jay Lundgren'; Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I truly hope that was humor about oversupply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called onesies (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling Faster, faster in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls on immunos
Fun no, impractical yes. Weems, Joyce jwe...@sjha.org 3/2/2011 10:30 AM The CAP guidelines are pretty clear. Copied from latest checklist.. Isn't this fun???! j:) ANP.22570 Phase IIN/A YES NO Are appropriate negative controls used? NOTE: Negative controls must assess the presence of nonspecific staining in patient tissue as well as the specificity of each antibody. Results of controls must be documented, either in internal laboratory records, or in the patient report. A statement in the report such as, All controls show appropriate reactivity is sufficient. A negative reagent control is used to assess nonspecific or aberrant staining in patient tissue related to the antigen retrieval conditions and/or detection system used. A separate section of patient tissue is processed using the same reagent and epitope retrieval protocol as the patient test slide, except that the primary antibody is omitted, and replaced by any one of the following: ■ An unrelated antibody of the same isotype as the primary antibody (for monoclonal primary antibodies) ■ An unrelated antibody from the same animal species as the primary antibody (for polyclonal primary antibodies) ■ The negative control reagent included in the staining kit ■ The diluent/buffer solution in which the primary antibody is diluted In general, a separate negative reagent control should be run for each block of patient tissue being immunostained; however, for cases in which there is simultaneous staining of multiple blocks from the same specimen with the same antibody (e.g., cytokeratin staining of multiple axillary sentinel lymph nodes), performing a single negative control on one of the blocks may be sufficient provided that all such blocks are fixed and processed identically. This exception does not apply to stains on different types of tissues or those using different antigen retrieval protocols or antibody detection systems. The laboratory director must determine which cases will have only one negative reagent control, and this must be specified in the department's procedure manual. The negative reagent control would ideally control for each reagent protocol and antibody retrieval condition; however, large antibody panels often employ multiple antigen retrieval procedures. In such cases, a reasonable minimum control would be to perform the negative reagent control using the most aggressive retrieval procedure in the particular antibody panel. Aggressiveness of antigen retrieval (in decreasing order) is as follows: pressure cooker; enzyme digestion, boiling; microwave; steamer; water bath. High pH retrieval should be considered more aggressive than comparable retrieval in citrate buffer at pH 6.0. It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen. The negative tissue control is processed using the same fixation, epitope retrieval and immunostaining protocols as the patient tissue. Unexpected positive staining of such tissues indicates that the test has lost specificity, perhaps because of improper antibody concentration or excessive antigen retrieval. Intrinsic properties of the test tissue may also be the cause of non-specific staining. For example, tissues with high endogenous biotin activity such as liver or renal tubules may simulate positive staining when using a detection method based on biotin labeling. A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered best practice (see below). 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250). Multitissue blocks may be considered best practice and can have a major role in maintaining quality. When used as a combined positive and negative tissue control as mentioned above, they can serve as a permanent record documenting the sensitivity and specificity of every stain, particularly when mounted on the same slide as the patient tissue. When the components are chosen appropriately, multitissue blocks may be used for many different primary antibodies, decreasing the number of different control blocks needed by the laboratory. Multitissue blocks are also ideal for determining optimal titers of primary antibodies since they allow simultaneous evaluation of many different pieces of tissue. Finally, they are a useful and efficient means to screen new antibodies for sensitivity and specificity or new lots of antibody
[Histonet] parts for CV5030 glass coverslipper
Does anyone know if you can buy these from someone else besides Leica? My Fisher rep never got back to me. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: RE: [Histonet] parts for CV5030 glass coverslipper
I'm not looking for repair parts. Just a place to order the filters that must be changed frequently. Sometimes, Fisher or VWR sell these type of consumables for Histo equipment. Victoria Baker bakevicto...@gmail.com 2/28/2011 12:26 PM Have you tried contacting Belair Instruments? I believe they are located in Fanwood NJ. Angie if you get a chance contact me off server :-). On Feb 28, 2011 11:04 AM, Sherwood, Margaret msherw...@partners.org wrote: We have a refurbished CV5030 coverslipper and we ordered directly from Leica. If someone knows of anyone else who sells the parts/supplies, I would also be interested. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, February 28, 2011 10:55 AM To: histonet Subject: [Histonet] parts for CV5030 glass coverslipper Does anyone know if you can buy these from someone else besides Leica? My Fisher rep never got back to me. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
We just had ours last week. We did well, but the inspecting Pathologist said the lab smelled like oranges we were in a way too cramped space. Go figure.. Akemi Allison akemiat3...@yahoo.com 2/23/2011 9:02 AM Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP
I did notice CAP inspectors concentrated more on safety this time around. Akemi Allison akemiat3...@yahoo.com 2/23/2011 11:50 AM We were given the new CAP checklist. I totally revamped our SOP Manuals, ALL QC forms, etc. to comply. This is the 1st Childrens Hospital CAP inspection I have undergone. We are inspected by other Children Hospital inspecting teams. After all the hard work, they did not go through any of our SOP's, QC manuals, Special Stains, validation protocols manuals for either histology or the IHC lab. Very odd indeed. They spoke with the AP manager for our department only, except to ask one of the histology techs how we disposed of our hazardous waste. Stay tuned till after the summation. Akemi Allison BS, HT(ASCP)HTL From: Jesus Ellin jel...@yumaregional.org To: Akemi Allison akemiat3...@yahoo.com; histonet histonet@lists.utsouthwestern.edu Sent: Wed, February 23, 2011 7:23:43 AM Subject: RE: [Histonet] CAP Akemi, were you inspected with the new CAP checklist or the old one? The situation in our lab is that we were given the checklist and then they changed us to the new format. Your thoughts -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Wednesday, February 23, 2011 7:03 AM To: histonet Subject: [Histonet] CAP Lot's of Labs in LA are in their CAP window! We had our CAP inspection yesterday and having our summation this morning at 9:00. I think our department did pretty good. Keeping my fingers crossed. Akemi Allison BS, HT(ASCP)HTL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] QIHC
I thought the NSH Self Assessment CD was helpful too. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Mark Tarango marktara...@gmail.com 2/10/2011 11:58 AM Its true, that test is too easy if you ask me. Dako handbook is all you'll need. Mark On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald ronald.hous...@nationwidechildrens.org wrote: Read and digest the Dako book and you will sail through the exam. For a specialist type qualification it really is pitiful. Ronnie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Bradley Sent: Wednesday, February 09, 2011 4:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi all I am preparing to take the QIHC exam. Does anyone have any suggestions on what I should study? What areas does the exam concentrate on and what research material I can use. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] High Complexity Testing
That was a hole in 1, Vikki. Viva la rant!! Victoria Baker bakevicto...@gmail.com 2/8/2011 11:58 AM The performance of ISH/IHC is in many laboratories considered high complexity testing - however - as the technologist doing the work does not make the diagnosis it is considered to be a part of specimen processing. (How's that for political double talking!) Use of automation in these procedures provides standardization of the protocols, but it is still a 'machine' it would not know how to recognize a staining or processing issue along with other possible faults. The purpose of automation was to provide standardization and consistent reproducibility of protocols, reduce antibody/reagent amounts, free the technologist for other needs in the lab and reduce turn around time required for this procedure. In essence it is hands off BUT not brains off. Short history is that when CLIA and CAP first started doing these ratings of low - high complexity testing Histology was a grey/special area in that the staff performed the technical aspect of the work, but did not sign off or make the diagnosis. Since then we have had revisions in CLIA and even with CAP guidelines there is still an aspect of subjectivity based upon the inspector's interpretation of the guidelines. It's frustrating, infuriating at and at times totally exasperating as you are trying to comply with all the requirements and regulations that are supposed to be clear. If your pathologist is considering all Histology work as low complexity I would wonder why they thought that? Is the lab participating in any of the CAP QA/QI programs? Does he/she participate in doing any In services with the staff? Do they interact with the staff and share anything with them in regards to the work or any specific concerns they may have? Does the department have reference material available to the staff for further study or understanding of the work they do and how important it is to patient care? Is the department willing to assist with $$'s for in-house CE courses or local Histology societies to further the laboratory staff development? One thing that I would wonder most is if they think that what you do is 'low complexity' could they go into the lab and perform the work themselves? Okay my morning rant is complete --- now I guess I should take my Estrogen ;-) If I have offended anyone, I apologize in advance. Hope everyone has a nice day. Vikki On Tue, Feb 8, 2011 at 10:06 AM, O'Donnell, Bill billodonn...@catholichealth.net wrote: I don't actually have an answer, but rather an observation. How many med techs are still doing glusoses in test-tubes, or manual drug screenings or hcg's? It would seem, by deduction that an automated glucose, if only because who it performing it, is a complex test. If measuring a bowel biopsy is now complex, why should the critical judgements and skills needed to cut a section, place it correctly on a slide and load the machine be considered not complex. If it comes down to who is doing it, then all of our efforts to elevate the field, gain higher and more competative wages and education requirements have done little except in isolated regions or laboratories. Morning rant Need to start getting more sleep. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a machine is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic
RE: [Histonet] Lab Assistants Embedding
Yes, I wondered about that reference too. This is embedding, not grossing that I'm asking about. Mike Pence mpe...@grhs.net 1/21/2011 9:49 AM Why, ANP11610 has to do with Gross Examination Qualifications for techs doing grossing. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, January 21, 2011 8:34 AM To: Mahoney,Janice A; Angela Bitting; histonet@lists.utsouthwestern.edu; histonet-reque...@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding If you are a CAP inspected lab, be sure to determine if your lab assistants qualify by meeting the guidelines in ANP11610. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, January 21, 2011 9:19 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu; histonet-reque...@lists.utsouthwestern.edu Subject: RE: [Histonet] Lab Assistants Embedding As long as you are in a state that does not require licensure for Histo techs you are fine. Just be sure that you have training and competency performed and documented according to CAP. We have done this successfully in my lab. Jan, Omaha, NE -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, January 21, 2011 8:01 AM To: histonet@lists.utsouthwestern.edu; histonet-reque...@lists.utsouthwestern.edu Subject: [Histonet] Lab Assistants Embedding Hello Ever-helpful Histofriends, I want to train my lab assistants to embed simple tissues like breast resection specimens, placentas, etc. My manager feels that this will break some kind of regulations and won't sit well with our doctoral staff. I feel pretty confident that other Labs are doing this and I'm ready to take up the torch, but I need some data from other hospitals. Are any of you utilizing your non-HT staff to do these tasks and what hoops did you have to jump through to get approval from the Doctoral staff? In addition, how does CAP look at this? Thanks for your help, as always, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site
[Histonet] Changing solutions on VIP processors.
Hello All, I'm involved in a debate with a colleague about how frequently the tissue processors need to be changed. We do roughly 1000 blocks a day on various runs, utilizing 7 tissue processors. About 100 of those are microwave processed, so I'm subtracting those from the equation. So 900 blocks (mixed tissues, no breast) divided by 6 conventional processors is 150 blocks each. I think it's poor reagent management to change a VIP after 150 blocks. Does anyone disagree with me? If these were processing very fatty tissue, I might think differently, but that's not the case. All opinions are welcome. Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Special Stain Automation
Just wondering how many people hate the Artisan's plastic well things that you have to clip on the slides?? Nails, Felton flna...@texaschildrens.org 12/1/2010 11:06 AM The problem with the Nexus is that you can not do silver and AFB on the same run. We use the Dako artisan with very consistent results and it is very easy to use. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Richmond Sent: Wednesday, December 01, 2010 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain Automation We are in the market for a new stainer to do special stains. We currently are using the Nexus and have been unhappy with it, particularly with the silver stains for which we do a lot of. I'm wondering what techs out there recommend for us to check into. - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC FILD
In my case, it was for my own personal gratification. Debora Probst histonet-boun...@lists.utsouthwestern.edu 10/15/2010 1:53 PM Can anyone tell me if once you have taken the IHC certification test and passed dose the administration consider that a specialty field and give you a pay increase? Or is it just for a persons own gratification to take it and pass? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOX-10
Happy Monday Histonetters, I'm wondering if anyone can share a protocol for running SOX-10 on the Ventana BenchmarkXT? (On FFPE tissue) Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parvovirus B19
Wondering if anyone is running Cellmarques Parvovirus B19 or Toxoplasma Gondii on a Ventana platform and would you mind sharing your protocol? Have a great weekend everyone! Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Powdered reagent expiration dates
Coincidentally, I had a question on this subject this morning. We rec'd in some oxalic acid and phoshotungstic acid, neither of which had expiration dates. I called Fisher and they looked it up for me and told me their expiration and offered to send it to me in writing, if I wanted. (it was 3-5 years from the manufacture date.) I was recently told by another tech that she thought 5 years was the shelf life of powders, but I wanted to see if that held true. Moral of the story is that it never hurts to call the manufacturer. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Pat Laurie foreig...@gmail.com 7/26/2010 1:27 PM We were inspected by CAP on friday and we were cited for ANP.21366 *Are reagents and solutions properly labeled, as applicable and appropriate, with the following elements?* * * 1. *Content and quantity, concentration or titer* 2. *Storage requirements* 3. *Date prepared or reconstituted by laboratory* 4. *Expiration date* Specifically that our staining powders didn't have an expiration date printed on the bottle. All of our reconsituted reagents which are in use were dated with an expiration date properly though. I have always assumed, perhaphs incorrectly, that powdered stains never expire. We have powders like Luxol Echt Blau, etc. that were purchased and opened over 40 years ago.If so, then these powdered reagents have gone through CAP inspections since the beginning and this inspector was the first one to find this problem. Is this one that we might protest? -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-215-5949 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Block disposal
They are classified as Pathological Waste. You should see what your state's rules are regarding Pathological Waste. histot...@imagesbyhopper.com 6/21/2010 12:33 PM Would blocks be considered biohazard waste for purposes of disposal? I have blocks stored in an off-site facility and am preparing to dispose of the blocks. Can I simply allow the storage place dispose of the blocks in normal trash, or would I need to have them disposed of as biohazard trash? On a separate but sort of related issue, does anyone use the Adlex crystals to dispose of their formalin? If yes, how do you dispose of the jellied formalin, in normal trash or in biohazard trash? I know the mfr says you can put it in the normal trash, but does anyone trust this?? ;o) By the way, I am in Florida, if that makes any difference in the answers! THANKS! Michelle ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab refrigerator
I'm looking for a new lab refrigerator for my IHC reagents. The techs would like to have shelves that pull out so the racks in the back aren't so hard to reach. We'd also like to have a double-wide model. Is anyone using one like this in their lab and would you tell me where you got it and the model #? Thanks and Happy Memorial Day All! Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana EMA antibody
Histofriends, Has anyone had success staining an ependymoma with Ventanas EMA antibody? My meningiomas stain nicely. I have a case that I've stained with Dakos EMA on an Autostainer, and it shows the expected spotty pattern. Anyone out there have a successful protocol? Happy Monday, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Artisan
Anyone know the approx. price of Dakos Artisan? Cost per slide? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Kaiser Permanente
If someone from Kaiser Permanente is watching the Histonet, would you contact me offline about a piece of Histology equipment. My rep. (and I'm not mentioning the company!!) is telling me you have something that I'm interested in buying, and I'd like your feedback. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Staffing for IHC
I'm hoping some of the larger hospitals out there will share their data with me. I'm curious as to how labs are staffed for doing IF/IHC/CISH. A small portion of our staining protocols still require pretreatment by hand, but we run instruments that do the pretreatments on board for the majority of our stains. We have 2 IHC techs, 1 on 1st shift and 1 on 3rd shift.Together they are cutting and staining between 200-350 slides per day. I think this is a high volume for only two people. What do you all think? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Floaters in Waterbath
We currently have a Quality Improvement Plan in effect to address this issue. Jackie is right about keeping those forcep wells clean. Although we don't swipe Kimwipes over our waterbath after each block, we do it very regularly. Another thing to consider is how often you clean your embedding molds. ~Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 ! Jackie M O'Connor Jackie.O'con...@abbott.com 10/23/2009 10:23 AM Kim wipes seem to pick up more debris than paper towels, and they pick up much less water. We routinely sweep the waterbath with a kimwipe after each block. You can also pick up floaters from embedding if the forceps are not cleaned between each block. Most embedding centers have multiple wells for forceps - how often do you clean those wells? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles estellamire...@gmail.com To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-boun...@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti-Tau on BenchmarkXT
I may have asked this before, but is anyone using BioCare's Tau antibody on their BenchmarkXT?? If so, would you be willing to share your experience with it? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thermometer vendor
Can anyone help me find where to order the short thermometers for use in the floatation baths? Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] water on slides?
We are having a very similar problem too. We change our alcohols and Histoclear like mad, but are having eosin bleeding out of the sections. I'm starting to wonder if it is the mounting media on our glass coverslipper. I ordered new bottle gaskets, so I guess I'll see what happens then. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Hayes, Tina J. tina.ha...@va.gov 8/24/2009 12:41 PM We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] GMS-Fungus on nails
Just wondering why you use Titebond II? We use plain old Elmer's Glue. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! jstaruk jsta...@masshistology.com 7/24/2009 11:41 AM Prepare a 10% solution of Titebond II premium wood glue (found in most hardware stores). Dip the slide in the solution just before mounting the section on the slide. Let the slide dry and stain away. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running several GMS stains before we might get a speck or two of tissue we can look at. We use the Artisan stainer for our specials. We are really not interested in performing the GMS manually, due to volume and turn around time restrictions. Any suggestions? Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for NYU contact
Just wondering if anyone can give me contact information for someone in the Neuropath Lab at NYU? Thanks Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Lean Consultants
Here's the website for consulting group we used: www.sprickstegall.com Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Anita Thorn ath...@bmhvt.org 5/12/2009 3:15 PM Are there any companies out there that go around helping set up LEAN Histology laboratories? If anybody can supply some company names I'd appreciate it. ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Job Openings
We have a part-time dayshift and a full-time night shift opening for a HT. If you're interested here's our web address. www.geisinger.org or call me directly. Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! eric.halp...@comphealth.com 5/12/2009 1:15 PM Good Afternoon- How do I review the job openings on the Histonet site? Eric Halpern Sr.Search Consultant, Lab Sciences and Imaging Division Permanent Placement Division CompHealth Associates, Inc. 6451 North Federal Highway, Suite 702 Fort Lauderdale, Florida 33308 Phone: 866-782-9029, ext. 5852 Cell 954-336-8913 Fax: 800-420-2329 email: eric.halp...@comphealth.com www.comphealth.com Please note: This is a commercial email from CompHealth Associates, Inc. If you do not want to receive future emails from CompHealth Associates, Inc. , please reply to the sender of this email and ask to be removed from our list. Customer service is the key to success. Are you satisfied with your experience? Send your comments to my manager at valerie.patter...@comphealth.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SMMS
We've been having trouble getting satisfactory staining for SMMS on our BenchmarkXTs. Is anyone getting a good result running these on the XT platform. If so, would you mind sharing your secret?? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gram AFB staining FNA smears
The subject of staining FNA smears with AFB and Gram stains in Histology vs in Micro came up today. Is there a reason that FNAs can't be stained the same way we stain our tissue sections? One of our docs was under the impression that it's not acceptable to use the staining methods we use in our Histology lab. I don't know what method Micro labs use, so I was hoping someone could shed some light on this subject for me. Thanks, as always, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Benchmark XT
I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. Joe Nocito jnoc...@satx.rr.com 4/8/2009 5:08 PM another issue could be the mixer blowing too hard. I've had that happen too. JTT - Original Message - From: Kari Bradshaw kbrads...@lcpath.com To: Dana Spears dspe...@mmci.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problemnot to mention several different lots numbers of slidesyikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbrads...@lcpath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] G 20 MEETING NEWS
Yes please. There are plenty of blogs out there for this kind of thing. Bernie Taupin bernietau...@ymail.com 4/1/2009 9:53 AM Please refrain from discussing politics on this list. Edwards, R.E. wrote: Apparently Obama and Brown, with the reluctant compliance of Germany and China are aiming to rationalise the pay and conditions of laboratory workers worldwide, and hopefully if they succeed the histotechs in the U.S.A. will one be the major beneficiaries!, so fingers xxxed everyone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histos-5
Anyone out there using the Histos-5 from Milestone? How much reagent does a Histomodule hold? I'm trying to figure out my return on investment using reagent savings. Do you really change your paraffin less often or is that just a sales pitch? How often do you change your paraffin and what is the volume of the paraffin tank? Thanks for your help once again, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] C4d Antibody
We get our from Alpco. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Cesar Francisco Romero chesar...@hotmail.com 3/24/2009 12:00 AM Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] help with muscle stains
Would anyone like to share their procedures for Cytochrome oxidase(COX), Succinic dehydrogenase (SDH) and NADH-TR with me? I have old, old ones but they are sketchy. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dako, Thermo and others
I'm pretty sure Danaher owns Surgipath now.along with nearly everything else. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Burton, Lynn lynn.bur...@illinois.gov 3/12/2009 11:46 AM Dako did acquire Surgipath recently. The Surgipath rep said it has been a good marriage for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. S. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu
RE: [Histonet] Dako, Thermo and others
Danaher owns them both. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Weems, Joyce jwe...@sjha.org 3/12/2009 11:53 AM Leica acquired Surgipath, not DAKO... j -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Thursday, March 12, 2009 11:46 AM To: Pamela Marcum; Victor Tobias; jeff Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others Dako did acquire Surgipath recently. The Surgipath rep said it has been a good marriage for them so far. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pamela Marcum Sent: Thu 3/12/2009 10:24 AM To: 'Victor Tobias'; 'jeff' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako, Thermo and others AMEN Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, March 12, 2009 11:02 AM To: jeff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako, Thermo and others Jeff, It is not just Dako, but many vendors have jacked up the cost of goods. Let's take the PSLIM from Accuplace. About a month ago you could purchase this for $5K. Now that Thermo has acquired distribution rights it costs over $10K and nothing has changed. To me, it is better to sell 10 at $5K than 5 at $10K. We were ready to purchase several more of these, but have to reconsider our options now. No wonder Healthcare costs are out of control. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. jeff wrote: I was wondering if anyone else is being blackmailed by Dako to sign a contract with them. We have enjoyed our relationship a number of years buying there product as we need it. We also have changed detection kits as we where advised. Now they are telling us that if we do not go with price per slide they will raise our costs of purchasing our supplies. Example EnVision + dual link system last year cost 1,580.70 this year 3,300 and that this will go in effect March 1st ( just talked to the rep about this Tues) Just 1 example. S. Has anyone else had this happen to them and what did you do? Thanks Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing
Re: [Histonet] BVD Antibody
I'm fighting a compulsion to make a bad underwear joke. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Breeden, Sara sbree...@nmda.nmsu.edu 2/24/2009 3:04 PM I need a source for BVD antibody and would appreciate suggestion. Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] on-line Histology programs
I have a Lab assistant who would like to become an HT. She is interested in OJTing here with us and taking the required classes on-line so that she can sit for her HT exam. Can anyone recommend a good program for her? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathos/ Milestone products
Does anyone have experiences with Milestone equipment, specifically the new KOS and Pathos Delta? Would you share your comments, pros and cons with me? Feel free to call me on the phone if you don't feel like typing a novel. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BioCare rep
Can anyone tell me how to get in contact with Kathy Bowden from Biocare? I left a message at Biocare but she didn't call me back. Maybe one of you has her email or phone number. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA job
Geisinger Medical Center in Danville, PA is searching for two Histotechnologists. We have a dayshift 0.6 tech position and a nightshift 8P-4A FT position available. Visit our website at www.geisinger.org to see more information about our Health System. Contact me for more information at 570-214-9634 or send me an email. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide drying
We use MM24 from Surgipath. We file our slides within 24 hours. I don't know if it will work in 12 hours, but maybe it's worth trying. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Martin, Erin erin.mar...@ucsf.edu 12/22/2008 2:06 PM Hello everyone, I was asked to find out how to dry slides quickly. They are glass coverslipped in an automated coverslipper at the reference lab we use and the our docs want them filed in less than 12 hours from the time they are coverslipped. We have been putting them in a 125 degree C convection oven for a few hours but the slides still get all stuck together in the file. They will not consider film coverslipping. Does anyone else file this quickly? I am grateful for any suggestions! Erin Martin UCSF Dermatopathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] protocols on Ventana
From what company do you order your PMS2, MSH2 and MSH6 concentrates? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Piero Nelva [EMAIL PROTECTED] 12/2/2008 2:52 AM I've deleted the original post, but to the person who was after Ventana protocols for Mismatch Repair proteins, here are ours: MSH2, 1:50, CC1 Mild, 37, 28 MSH6, 1:4000, CC1 Std, 37, 40 plus amplification kit MLH1, Vetnana predilute, CC1 Mild, 42, 28 plus amplification kit PMS2, 1:100, CC1 Mild, 37, 56 plus amplification kit. I can send you some images of our results off list if you like Regards Piero Nelva Anatomical Pathology Monash Medical Centre Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:[EMAIL PROTECTED] N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] breast bx processing
We have staff in on Sunday afternoon, so we start them Friday night and let them sit in paraffin until the techs come in on Sunday. The extra paraffin has actually cut down the number of dry outs we were having. Martin, Gary [EMAIL PROTECTED] 11/17/2008 2:14 PM We come in and process them ! -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Margiotta, Michele Sent: Monday, November 17, 2008 9:37 AM To: [EMAIL PROTECTED] Subject: [Histonet] breast bx processing Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] President
not really...just want to keep this arena of scientific thinking sacred (and totally non-partisan)! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Joachim Hehl [EMAIL PROTECTED] 11/5/2008 10:58 AM Interesting, the histonet list seems to be occupied by ultraconservatives. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:[EMAIL PROTECTED] N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] progressive HE staining using Gills
We just switched our HE stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:[EMAIL PROTECTED] N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] barcode labels vs handwritten
Hi Folks, I am running into resistance with a few of our pathologists regarding the use of barcoded labels. They want us to handwrite our slides and then apply our permanent labels over top of that. We are using the Stainershield labels that are solvent-resistant. We eliminated the step of relabeling our slides with the permanent labels at the sorting area, and began having the cutter label their slides with the permanent labels at the microtome area as part of a Lean workflow initiative. However, a few of our Pathologists like having the handwriting underneath the label so they can see who cut the slide if it is of poor quality. Also, if they think it is mislabeled they say that gives them a clue as to where the slide really came from. I guess I feel like, if the slide is mislabeled it doesn't matter where it came from, it still needs to be recut from the correct block. I'd appreciate your comments and suggestions about this issue. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
