Re: [Histonet] cleaning old charged slides

[Atoska Gentry via Histonet]

Hello,

Has anyone had success cleaning old charged slides which have become foggy over 
time.

Thanks!
~Atoska
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Re: [Histonet] Leitz Rotary Microtome

[Atoska Gentry via Histonet]

Hello,
Does anyone have experience with a Leitz Minot Rotary Microtome No. 1212?
I'm particularly interested in a source for troubleshooting problem sectioning.
Thanking you in advance for your assistance.

Best Regards,
~Atoska
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Re: [Histonet] Histonet Digest, Vol 188, Issue 17

[Atoska Gentry via Histonet]

Hello Sandra, 

Following are catalog #'s for Fisher substitutes that I received from their 
site: 12-545GP (50x35mm); 12-548-5RP (45x50mm). 
Ted Pella, Inc.  Gold Seal 3327
www.expressmedicalsupply.com Erie-Scientific ER 35x50-1.5 &  ER 45x50-1
Also try Brain Research Laboratories 4860-1 (48x60mm) although they're a bit 
more pricey.
Thomas Scientific (48x60mm) #6672-A64
Additionally, I've seen some eBay listings.

Best wishes,

~Atoska

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Wednesday, July 24, 2019 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 188, Issue 17

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Today's Topics:

   1. Jeepers Creepers, Coverslipping Peepers! (Sandra Cheasty)
   2. Re: [External]  Jeepers Creepers, Coverslipping Peepers!
  (Dessasau III, Evan)
   3. Two part Special Stain wash (Cassie P. Davis)


--

Message: 1
Date: Tue, 23 Jul 2019 19:49:21 +
From: Sandra Cheasty 
To: "Histonet (histonet@lists.utsouthwestern.edu)"

Subject: [Histonet] Jeepers Creepers, Coverslipping Peepers!
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Hello all,
Does anyone have a source for oversized coverglass? (35x50, 
35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, 
some of which are from horses, cows, etc. that are put on the larger slides. 
Fisher used to carry their own brand, but it's apparently discontinued them, 
and their "alternate" product is $50/oz!
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine



--

Message: 2
Date: Tue, 23 Jul 2019 20:46:38 +
From: "Dessasau III, Evan" 
To: Sandra Cheasty 
Cc: "'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] [External]  Jeepers Creepers, Coverslipping
Peepers!
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Hi Sandra, Have you tried Brain Research Laboratories?  We use the 35x50mm 
no.1, 3oz. pack.  We buy them through Mark at PathSupply.  The phone number on 
the box is 617 965 5544.  PathSupply's number is 800 631 3556.
Thank you,

E-van

-Original Message-
From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, July 23, 2019 3:49 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [External] [Histonet] Jeepers Creepers, Coverslipping Peepers!

Hello all,
Does anyone have a source for oversized coverglass? (35x50, 
35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, 
some of which are from horses, cows, etc. that are put on the larger slides. 
Fisher used to carry their own brand, but it's apparently discontinued them, 
and their "alternate" product is $50/oz!
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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--

Message: 3
Date: Wed, 24 Jul 2019 16:41:14 +
From: "Cassie P. Davis" 
To: histonet 
Subject: [Histonet] Two part Special Stain wash
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"

Hi Histonet folks,

  If you still have the NEXES special stainer do you use the two part wash  
cat#860-040 or the 10x concentrate cat#860-015?

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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information that is privileged and 

[Histonet] mouse pituitary

[Atoska Gentry via Histonet]

Hello,

does anyone have a tried & proven method and/or pointers  for mounting mouse 
pituitary for cryo sectioning which guarantees great cross sections?

Thanks!

~Atoska

Sent from Mail for Windows 10

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Re: [Histonet] blades

[Atoska Gentry via Histonet]

Hello does anyone have a source for Personna Single-edge stainless steel razor 
blades? Our former vendor no longer stocks them. Thanks, Atoska
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Re: [Histonet] specimen storage cabinets

[Atoska Gentry via Histonet]

Hello, I work for a research facility and we currently have an archive of 
several formalin fixed samples stored in 500ml glass specimen jars; one of our 
PI's recently asked me to conduct a search for storage cabinets specifically 
designed to accommodate these samples. Is anyone aware of the availability of  
such cabinets? The only thing my search has turned up thus far are the 
flammable metal cabinets. But due to limited storage space we have to stack 
these jars and the generic flammable cabinets are not stacked glass specimen 
jar suitable. All ideas & input are welcome. Thank you kindly, Atoska
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Re: [Histonet] Automated Slide Stainers

[Atoska Gentry via Histonet]

Hello, one of my department's researchers is in need of an automated slide 
stainer which accommodates 3"x2" microscope slides. If you have experience with 
such will you please contact me at your earliest convenience? Regards, Atoska 
Gentry
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