Re: [Histonet] Slide remounting
Hello Soak slides in xylene or equivalent solvent for sometimes till the coverslip is loose, carefully remove the coverslip; you can remount the slides with Permount. You can dissolve the Permount in organic solvents, but the glue will be dissolved and you have to remount the slide. Bader On Wed, Aug 27, 2014 at 8:12 AM, Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US) juan.l.bassett@mail.mil wrote: Does anyone have a procedure that removes coverslips (w/crystallized permount present) ? The slides are 10+ years old. And is there a process to liquify the crystallized permount and refill w/o removing the coverslip? The mounted slides are 3 x 1's Juan l. Bassett HT ASCP Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 juan.l.bassett@mail.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて muito agradecido/a(s) Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Website: www.ImmunoBioScience.Com *E-mail: bade...@gmail.com* Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 Cell (Mobile) phone: + 1 425 314 0199 Address: PO Box 218 Mukilteo, WA 98275-0218 USA Marketing person: Anita E-Mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Hello Methanol is added to reagent alcohol (ethyl alcohol) so that people do not use for drinking purpose and for tax purposes. Alcohol (ethyl alcohol) has federal tax just like liquor. One can buy pure alcohol, but you have keep inventory and make sure it is not misused. Bader On Tue, Jul 17, 2012 at 1:28 PM, Rene J Buesa rjbu...@yahoo.com wrote: To prevent oxidation of those 2 reagents René J. From: Kelly Boyd kdboydhi...@yahoo.com To: histonet Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 17, 2012 1:16 PM Subject: [Histonet] (no subject) Since there is discussion of formalin and methanol, I would like to ask everyone.Why would a vendor add methanol to their reagent alcohol and their 10% buffered formalin? What are the advantages/disadvantages to the tissue especially for processing? Kelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Invitation to connect on LinkedIn
LinkedIn Bader Siddiki requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Bader Accept invitation from Bader Siddiki http://www.linkedin.com/e/yvpgd1-gxm7sa8y-5z/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I212940440_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYMd3gMd3AOcj99bTlfkA9mlkVvbPoMdzkPdjkUdjkLrCBxbOYWrSlI/EML_comm_afe/?hs=falsetok=0e1VA57-BVul41 View invitation from Bader Siddiki http://www.linkedin.com/e/yvpgd1-gxm7sa8y-5z/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I212940440_13/3cNnP0Qd30Qej8NcAALqnpPbOYWrSlI/svi/?hs=falsetok=0Nno1M-mxVul41 -- Why might connecting with Bader Siddiki be a good idea? Bader Siddiki's connections could be useful to you: After accepting Bader Siddiki's invitation, check Bader Siddiki's connections to see who else you may know and who you might want an introduction to. Building these connections can create opportunities in the future. -- (c) 2011, LinkedIn Corporation ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] We need someone to test two antibodies.
Hello Histonetters We are in need of someone who can test two antibodies: CD71 and CD74 for us. --IHC Frozen Tissue (Methanol fixed tonsil) for both antibodies. --ImmunoFluorescence - Frozen tissue (Methanol fixed) We have FITC conjugated antibodies. Please contact us at : bade...@gmail.com If you need more information, please visit our web site: www.ImmunoBioScience.com and look for monoclonal antibodies catalog # MM-1027 and MM-1029 Thank you. Bader -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene
Hello Histonetters I have been reading about xylene, how dangerous it is. Have you thought about using mounting medium made from Limonene. Here is some info: *AR-6504 Organo (Limonene) mount™:** *This mounting medium is made with limonene a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in Immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. This Organo mounting medium is also suitable with alkaline phosphatase chromogens, an organic solvent resistant Super Fast Red (IBSC, cat AR-8211). It is an excellent choice for mounting H and E stained slides. Coverslip is required. *DIRECT SUBSTITUTE OF Biomeda’s Clarion* This mounting medium is made by ImmunoBioScience corp. www.ImmunoBioScience.Com Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Light + IF microscope
Hello Histonetters I need your professional advice. We are a small IHC and IF company looking for a good microscope. We have investigated in Nikon;s they have 50i and 55i microscope that one can use for IHC and IF with proper attachment, but pretty steep price. Do you have any recommendation for a good quality microscope w/ IF attachments. May be one of you have a microscope like this and want to sell at a reasonable price. Your expert advice and recommendation will be appreciated. Thank you. Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rcd e-mail on Histonet regarding a good place to get eletronics etc.
Hello Histonetters I hope you guys and gals are getting ready for the weekend, have a good one. Few months ago we received an e-mail on histonet that there was a place on internet one can purchase electronics at reasonable prices. Did any order anything from them, are they for real. I lost that e-mail and web site address, if you have this info. We Bader Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mounting medium for IF
Hello Laurie Pi gives red color in IF, it stains DNA and RNA PI excites at 535nmand emits at 615nm, producing a *red* fluorescence. Therefore if you are using any chromophore at these wave lengths which emits red color you will have problems. By the way we also have several mounting mediums for IHC and IF, if you would like to try, please let us know. Bader ** -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] opinion please
Hello I have the same experience. When I worked for a company and right after 9/11, we shipped antibodies to Israel via Post office at ambient temp. This package came back after 6-8 weeks or may be longer. We do not know how they were stored at post office. We tested the antibodies and could not find differences in the activity using IHC. Bader On Mon, Dec 7, 2009 at 4:02 PM, Akemi Allison akemiat3...@yahoo.com wrote: I agree with Tim. This is a very common practice for antibody manufacturer's. Also, manufacturer's will revalidate antibodies when they are close to their exploration date and if that antibody has the same sensitivity. They will extend the shelf-life date. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Dec 7, 2009, at 2:32 PM, Morken, Tim wrote: Kim, Test a random sampling (10%) of the antibodies and see if they work. If so, then they are most likely fine. Document the test. Antibodies are very robust and the warmup probably won't damage many, if any, of them. BTW, When I worked for an antibody manufacturer I did a test once as part of a stability problem investigation and left six different antibodies in a 37C oven for up to 4 months. We tested a sample of each weekly. All worked fine and some even worked better even after 4 months! The issue I would be concerned about is bacterial growth in any of them. Keep an eye on that. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Monday, December 07, 2009 6:51 AM To: Histonet Subject: [Histonet] opinion please Help! My refrigerator died over the weekend. This fridge had most of my antibodies and IHC reagents. When I came in, the temperature inside the fridge was a balmy 37C! Do you think any of my reagents are still usable? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plants in Histology lab
Everybody is writing about the plants in the histology lab. May be I will share my experience with you in Biochemistry lab at MSU . Many many years ago when I was a post doc in Biochemistry dept. I had lots of plants in the window. One day when I came from lunch, I saw unusual plants along with mine, on close look, they were marijuana plants. I almost had heart attack to see these plants in my cubicle, right away I hid these plants. On investigation we found that our dish washer had these plants some place in the lab. and she was hiding it from the professor. Bader ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] need help in selecting blades for Microtome Leitz1512 and good place for service
Hello Histonets I need some help and advice from you experts. We are new in cutting formalin fixed paraffin embedded (FFPE) tissue sections. We need your advice. We have a used Leitz 1512 microtome. --- perhaps it needs service, can you recommend a good and reasonable place. what will be a good disposable blades to buy for cutting FFPE tissues sections (mostly soft tissues like colon, stomach, skin, cancer tissues etc). this instrument needs a blade holder, what would you recommend, Leitz does not sell blade holders for this instrument anymore. any good technique for cutting tissues for a person who is not expert. good place to buy formalin fixed tissue blocks for research use. Thanks a million. Bader e-mail address: bade...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome Leitz 1512, knife/blade holder
Need help, looking for knife/blade holder for Leitz 1512 Microtome. Does anybody know where to buy this, thanks Bader e-mail address: bade...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Fluorescent dyes and xylene
Hello My experience is that most Immunofluorescence dyes do not withstand alcohol and organic solvents dehydration. You can use aqueous mounting medium with anti-fading agent. Frozen brain tissue is tough to work with; since it contains tons of lipids. Lots of mounting mediums will produce air bubbles because aqueous mounting medium being hydrophilic and brain tissue being hydrophobic. We are in the process of developing an aqueous mounting medium with anti-fading agent and should be OK to use with tissue like brain, if interested please let us know we can send you a sample for evaluation. Bader On Mon, Jul 20, 2009 at 11:22 AM, Bob Nienhuis bob.nienh...@gmail.comwrote: Sorry, I should have mentioned that this will be done on thick frozen sections. Bob On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis bob.nienh...@gmail.com wrote: Will the Alexa Fluor dyes withstand some dehydration and clearing in alcohol and xylene? If they won't are there any bright fluorescent dyes that can be obtained conjugated to a secondary antibody that will? Looking to immunostain tyrosine hydroxylase in rodent brain with a fluorescent marker in tissue that needs to be dehydrated and cleared, perhaps with an abbreviated series. Bob Nienhuis VA / UCLA Neurobiology Research ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne / 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience corp. Phone: + 425 367 4601 Phone: + 425 514 3761 Fax: + 425 367 4817 cell (mobile) phone: + 425 314 0199 e-mail address: bade...@gmail.com Web site: www.immunobioscience.com Marketing: phone: + 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
