RE: [Histonet] Paraffin Accumulation on floor
We have a long-handled scraper and use it periodically. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, February 24, 2010 1:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Accumulation on floor How is everyone dealing with the accumulation and tracking of paraffin problems. We have mats on the floor where we cut and embed. We also have the mats that you walk on and it takes the paraffin off of the bottom of your shoes and you peel off a layer a day. Our medical director wants us to find a way to keep the floors clear, so that no one will slip and fall. I have never seen a histololgy lab that did not have a paraffin tracking problem. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] See you at the NSH
I'll be looking for you! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, September 24, 2009 7:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] See you at the NSH Hi all, Just a note to say that I will be leaving next week for Birmingham Alabama for the NSH conference. It has taken me 30 years to get to a NSH and I am really looking forward to it. I hope to see you all (I will be the old, short, bald guy with the Aussie accent) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA - * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cold trays
Sakura carries them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of maureen bukhari Sent: Tuesday, September 22, 2009 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cold trays Does anyone out there know where I can find a fridge-tray or a cold plate to cool wax blocks while cutting Maureen Bukhari Phone: 403-210-6524 e-mail: mlbuk...@ucalgary.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Products
We have the embedding center and would highly recommend it -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Butte Sent: Tuesday, August 25, 2009 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Products Hi All: I'm looking to purchase equipment for a new lab and would like the opinion of anyone who has worked with various Sakura products before... good or bad. I would be looking to buy the following products: Tissue-Tek TEC 5 Embedding Station Accu-Cut SRM 200 Microtome Cyto-Tek Cytocentrifuge Also if anyone has experience with prostate and GI bx processed in the Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that too. Thanks! -- Emily Butte, M.S., HTL (ASCP) Path Lab Solutions Inc. (701) 371-7515 em...@pathlabsolutions.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] refrigerating sliver and gold chloride??
I know when commercial Schiff's was no longer required to be kept refrigerated I had my doubts but it worked fine. I am guessing this will be the same. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, August 21, 2009 12:09 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-boun...@lists.utsouthwestern.edu Subject: [Histonet] refrigerating sliver and gold chloride?? I just noticed that my silver and gold chloride says to store at room temp now. I have always stored them in the refrigerator. Is anyone still doing this?? Old habits die hard. I want to know if this has caused any staining issues for anyone. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disinfectant
Perhaps this will help. http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5404a2.htm Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, August 20, 2009 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disinfectant Could anyone please assist me in locating specific references for disinfecting work surfaces in the laboratory? I'm looking for something from the CDC or OSHA regarding this issue. Thank you in advance! Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lab Telephone
When our new lab was built they gave us phones that could only be used for dialing out...and none in the smaller, procedure rooms. We argued that the tissue processors and other instruments were often in these procedure rooms and it would be handy if we needed a service tech to walk us through a problem. We ended up with the phones in the procedure rooms and also a phone that allowed incoming calls in the main lab. That way we could call the lab looking for individuals without having to run around looking for them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, August 18, 2009 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab Telephone I'm having a challenge wrapping my head around this one: my boss wants to know why I need a telephone in my new lab in the new building we're moving into in January. Part of this is cost (what isn't a cost issue these days??) but I am to come up with justification for having a telephone IN the lab. The new phones will be cordless but he wants the phone to be in the hallway for what he deems a safety issue (I am not a BSL3 lab). The new phone system will be individual lines for specific locations within our facility. I'm the only tech and I'll have a 22x22' lab and the adjoining (with separate entrance) storage room/volatile storage and I will be doing specimen cut-in in another area altogether. I know I've left out some salient points so if you need more info AND if you have some logical input, please ANSWER offline to me directly. I can't figure out why I would NOT need a telephone... egad! I was asked how many calls (in/out) I make/receive per month as a basis for the decision. OMG. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NSH Meeting Hotels
And when I made my reservation at the Sheraton the NSH rate they had was more expensive than what the NSH website showed. Not much, but it was higher. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 13, 2009 9:45 PM To: histonet Subject: [Histonet] NSH Meeting Hotels Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium. I went to the NSH website and looked under the Hotel Travel icon and saw that the only hotel that wasn't Sold Out was the Double Tree. I called and they never heard of us NSH. I read all the information to the reservation person and she came up with a blank. I requested the supervisor. After searching for about 15 minutes, she found us under histotechnology. Anyone trying to book a room should state that our GROUP CODE is CI0N Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Tissue Tek Xpress
I love our Xpress! Our lab is certainly not typical...we are very different from a routine hospital lab. We are an infectious diseases group so primarily receive liver tissue (both autopsy and biopsy), skin biopsies, kidney, lung, GI...pretty much everything other than breast, uterus, prostate and the like. We do not work a night shift so our justification for the instrument was primarily for outbreaks, urgent cases, etc. It saves a huge amount of time when it is critical for us to get results ASAP. Our IHC are at least as good as with traditional processing and in some cases better. Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider Sent: Monday, July 27, 2009 3:19 PM To: histonet Subject: [Histonet] Sakura Tissue Tek Xpress My group is considering the purchase of the Sakura Tissue Tek Xpress Continuous Rapid Tissue Processor. Can any Histonetters give me feedback on their experience with this instrument? I'd like to hear the good and the bad. Can you justify the cost? How did you modify your staffing schedule? Does it produce quality results on biopsies? and on surgicals? and fatty tissue? How does it effect IHC? Feel free to reply on the Histonet, or, if you'd prefer, you may email me privately. Thank you! Daniel Schneider, MD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Start Up Lab
Thanks to spell-check..it self-corrected me..incorrectly! Thanks for setting it right. -Original Message- From: Smith, Allen [mailto:asm...@mail.barry.edu] Sent: Thursday, July 23, 2009 12:26 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Start Up Lab I can't imagine why any one would use dioxin, which used to be used as an insulator and heat convector in electron microscope transformers, as a clearing agent. I think you mean dioxane. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, July 23, 2009 12:13 PM To: Edwards, R.E.; Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Hah! Sure do. -Original Message- From: Edwards, R.E. [mailto:r...@leicester.ac.uk] Sent: Thursday, July 23, 2009 11:51 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab You still have fingers!?. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 23 July 2009 16:44 To: Montina Van Meter; Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab And I used to use dioxin in my tissue processor and would actually dip gauze in it to clean paraffin off my fingers. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Montina Van Meter Sent: Thursday, July 23, 2009 11:31 AM To: Carol Bryant; theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab I can remember back in the late 70's sitting at my microtome in a clinical lab (that was the size of an elevator shaft = no ventilation),where my supervisor would puff away on cigarettes with an ashtray perched on top of her microtome. When we had inspections everyone would put their contraband in their personal drawer. I also interned in an ENT lab that processed with celloidin (=ETHER) and didn't have windows or a hood. That supervisor also smoked in the lab! I said a prayer every day that I wouldn't blow up or be asphyxiated during my two week stay. Montina J. Van Meter, HT (ASCP) Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2564 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, July 23, 2009 9:04 AM To: theci...@yahoo.com; Lynette Pavelich Cc: Histonet Subject: RE: [Histonet] Start Up Lab Love it! Too funny! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of theci...@yahoo.com Sent: Wednesday, July 22, 2009 6:59 PM To: Lynette Pavelich Cc: Histonet Subject: Re: [Histonet] Start Up Lab Dang maybe I should stop keeping my lunch in the cryostat. The fresh unfixed tissue adds a certain je ne sais quoi to the flavor. :/ Sent from my Verizon Wireless BlackBerry -Original Message- From: Lynette Pavelich lpave...@hurleymc.com Date: Wed, 22 Jul 2009 18:07:07 To: lei...@buffalo.edu; histonet@lists.utsouthwestern.edu; cing...@uwhealth.org Subject: RE: [Histonet] Start Up Lab Gosh.I remember the days sipping on my coffee and nibbling on a fresh donut as I cut my morning slides! Sigh.. Merced M Leiker lei...@buffalo.edu 07/22/09 5:00 PM (lol some labs have a bench area as well as a desk area where food is allowed.) --On Wednesday, July 22, 2009 3:19 PM -0500 Ingles Claire cing...@uwhealth.org wrote: In the lab?!? For shame. :) From: histonet-boun...@lists.utsouthwestern.edu on behalf of Cindy DuBois Sent: Wed 7/22/2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Start Up Lab And a coffee pot. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However
RE: [Histonet] Leica Peloris versus Microwave Processing
We use the Sakura Xpress with no problems. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna Willis Sent: Tuesday, May 19, 2009 9:55 AM To: Carol Bryant; histonet-boun...@lists.utsouthwestern.edu; Mr. Jim Vickroy; Histonet Cc: Dave Sanford; Jes Strong Subject: Re: [Histonet] Leica Peloris versus Microwave Processing No issues using the Milestone products. Donna Sent via BlackBerry by ATT -Original Message- From: Carol Bryant cb...@lexclin.com Date: Tue, 19 May 2009 09:35:54 To: Vickroy, Jimvickroy@mhsil.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Peloris versus Microwave Processing Are there any issues with microwave processing and IHC? Thanks, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, May 19, 2009 9:25 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica Peloris versus Microwave Processing We are thinking about purchasing a new tissue processor. We have looked extensively into microwave tissue processors instead of the conventional tissue processors we are all used to. Recently two of my techs came back from a symposium and brought me literature on the Leica Peloris. The brochure makes some statements that it is faster than microwave processing. Has anyone used this processor and what has been your impression? Also do you have any opinion on how it matches up with microwave processors such as the Sakura Tissue Tek Express or the microwave processor by Milestone? Any input would be appreciated. Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by the State of Kentucky and/or Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient and have received this communication in error, please contact the sender and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome Alignment
Newcomer sells them. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Thursday, May 07, 2009 9:24 AM To: histonet Subject: [Histonet] Microtome Alignment Does anyone know where I can an alignment device? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AMMONIUM HYDROXIDE
Phyllis, I have used ammonia frequently in the past for soaking certain troublesome blocks but it has never resulted in sections washing off. We do use Plus slides, though. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Monday, April 27, 2009 10:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] AMMONIUM HYDROXIDE Hi all, I looked in archives to see if I could find any info on this but did not. Remember, we are having a problem with some of the sections floating off during IHC. If paraffin blocks are faced and then placed in ammonium hydroxide before cutting the HE, would this cause the sections to float off if the unstained sections that are cut in addition to the HE's are also produced in this manner? Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fontana Masson
I love the one from American Mastertech. www.americanmastertech.com Product code: KTFMA Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Putnam, Jodi Sent: Monday, April 27, 2009 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fontana Masson Hello. Do any of you know of a source for a kit for the Fontana Masson? I am used to doing it on the bench and making up all the solutions, but under the circumstances, a kit might be better. I have checked Sigma and Newcomersupply so far and they don't have kits. Any help would be greatly appreciated. Thanks! Jodi Jodi Putnam (HT,ASCP) Graves Gilbert Clinic Pathology Department 201 Park Street Bowling Green, KY 42102 (270) 393-2728 (voicemail) (270) 393-2795 Fax : (270) 393-2736 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Graves-Gilbert Clinic. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] email
You must go to the Histonet site and follow the instructions to remove yourself. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nan Wang Sent: Thursday, April 16, 2009 10:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] email Hi, Can you remove me from the email list? Too many emails. Thanks. nan wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Stamp???
It's called a tamper and I know Fisher sells them.search embedding tamper on the web site. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yeonju Shim Sent: Tuesday, April 14, 2009 9:54 AM To: histonet; histonet-ow...@lists.utsouthwestern.edu Subject: [Histonet] Embedding Stamp??? Hi, I am trying to order a tool that I can flatten wavy tissues down at the bottom of the mold for embedding. It looks like a little metal square (kind of) stamp. Do anyone know what it's called and where I can order? Thank you, Judy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Staying...
Amen! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marla Thomas Sent: Tuesday, April 07, 2009 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staying... I am staying on the list-serve. I have learned a lot from people on this list-serve. I have been in the field since 1970 and I too am still learning things. No one has all of the answers, and sometimes there isn't just one answer. I am staying on the list-serve, and if my input can help one person, or 15 different responses can help me, then the list-serve works. There are still people out there asking questions in between the bickering. Marla Thomas, HT(ASCP) Litton Pathology Associates, PC 700 NW Hunter Dr. Blue Springs, MO 64015 Phone: 816-229-6449 Fax:816-874-4400 CONFIDENTIALITY NOTICE This message and any included attachments are from Litton Pathology Associates, P.C. and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 816-229-6449 and ask for the HIPAA/Compliance Coordinator. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fc receptor blocker for staph
Does anyone know where you can purchase an Fc receptor blocker for staph? Vendors welcome! Thanks! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] onalighternote
I enjoy the lighter side of things in my crazy day.and the subject line warns us ahead of time -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 9:51 AM To: Pat Flannery Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote Then maybe we're really making fun of the other Bernie; we just didn't know it. :-) Perhaps you should get back to your histology and just plain stop making fun of anyone, for any reason. From: Pat Flannery pjfne...@duke.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:20:30 AM Subject: Re: [Histonet] onalighternote Then maybe we're really making fun of the other Bernie; we just didn't know it. :-) I don't know what makes some of you people persist in making fun of a complete stranger's name, but I'll have you know I was born before the person you are talking about. From: Va Paula Sicurello vapat...@yahoo.com To: Jeanine (CDC/CCID/NCZVED)Bartlett j...@cdc.gov; R.E.Edwards r...@leicester.ac.uk;histonet@lists.utsouthwestern.edu; Bernie Taupin bernietau...@ymail.com Sent: Tuesday, March 31, 2009 7:01:14 PM Subject: Re: [Histonet] onalighternote Hey, we can't help it if your parents were big fans of his. He is a great song writer. ;-) Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Research Imaging Center 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 --- On Tue, 3/31/09, Bernie Taupin bernietau...@ymail.com wrote: From: Bernie Taupin bernietau...@ymail.com Subject: Re: [Histonet] onalighternote To: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov, Edwards, R.E. r...@leicester.ac.uk,histonet@lists.utsouthwestern.edu Date: Tuesday, March 31, 2009, 4:52 PM Yeah, hilarious.. I swear, I haven't heart THAT one before From: Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov To: Edwards, R.E. r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:18:36 AM Subject: RE: [Histonet] onalighternote I wanted to ask how Elton was but thought that was a bit too easy... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] onalighternote
Hey Bern, I'm with you! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 10:03 AM To: Robyn Vazquez; Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote Please stop replying and use your own advice. Histology talk anyone? I'm curious- Why didn't you seem to feel this way about the first 3-5 replies that were sent out making fun of my name, completely devoid of histology talk? From: Robyn Vazquez vazqu...@ohsu.edu To: Bernie Taupin bernietau...@ymail.com; Edwards, R.E. r...@leicester.ac.uk; histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 9:57:53 AM Subject: RE: [Histonet] onalighternote Please stop replying and use your own advice. Histology talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 6:55 AM To: Edwards, R.E.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record?? I would try to reply to this, but I have no idea what s are. From: Edwards, R.E. r...@leicester.ac.uk To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, March 31, 2009 10:15:51 AM Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Off topic...lets call a truce
It's not just you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 01, 2009 7:09 PM To: Amy Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Off topic...lets call a truce What I have read has not been offensive or rude by anymeans towards any one individual he thinks he needs to retaliate by insulting others way worse that the original comments had been is it just me, or do these two statements sound quite contradictory? From: Amy Johnson ajohn...@aipathology.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 1, 2009 4:00:37 PM Subject: [Histonet] Off topic...lets call a truce Histonetters, I have been watching and reading these posts all day and I find it all very hilarious. What I have read has not been offensive or rude by any means towards any one individual yet there is one out there who thinks he is being attacked and therefore he thinks he needs to retaliate by insulting others way worse that the original comments had been. Can this all be for real or have I just got caught in an April fools day joke. Just wanted to get in on the fun. Amylin Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] onalighternote
I wanted to ask how Elton was but thought that was a bit too easy... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Tuesday, March 31, 2009 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] onalighternote I have just had s from Bernie Taupin and Paul Schofield, is this a record??. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: 31 March 2009 15:08 To: Bernie Taupin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ford Royer I think Ford gets the message...GET ON WITH IT! Antibody talk anyone? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Tuesday, March 31, 2009 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer I can personally attest that Ford must have been having a VERY bad day indeed. Although I can empathize that this might be the case, I do find it curious that we've heard nothing from Mr. Royer since his little outburst. I, for one, wouldn't mind an apology to the list for your antisocial behaviour on it, Mr. Royer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave tissue processor
We have had great success with our Sakura Xpress. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, March 31, 2009 11:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] microwave tissue processor We are thinking about Microwave Tissue processors. Unfortunately I have been given very little time to present a budget request. Can anyone comment on a recent purchase of a microwave tissue processor and their experiences? Thanks Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Please help! In dire need of user manuals
Double ouch! Somebody has their panties in a bunch today! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, March 27, 2009 11:53 AM To: fro...@bitstream.net; Jennifer Anderson Cc: Histonet Subject: Re: [Histonet] Please help! In dire need of user manuals OUCH JTT - Original Message - From: fro...@bitstream.net To: Jennifer Anderson jander...@halozyme.com Cc: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 26, 2009 9:50 PM Subject: Re: [Histonet] Please help! In dire need of user manuals Dear Jennifer and any other Histonetters that are of like mind, It's not quite Flaming Friday yet, but it will be in just a few hours, so I'll go ahead a little early with what I hope is a reply that may enlighten, be consultative, and hopefully not too offensive to those that may be sensitive to such replies. Here it goes... My First Question: You state that your lab has purchased all used equipment. What vendor did you purchase from that did not provide operator's manuals with the equipment? This is unheard of amongst us reputable used/refurbished equipment dealers so if I were you, I would not recommend this vendor to others. Now let's make sure that I have the facts straight... You have posted a request that is seeking charity from members of this list that also includes vendors. In addition, not only are you asking for free copies of publications that have been copyrighted and are the proprietary information of the manufacturers that, at great RD expenditure developed them, but you are asking that the donor(s) mail hard copies (if available) to you ...at the donor's expense for the shipping handling. Should hard-copies not be available, you request the donor(s) to provide these lengthy documents in digital PDF format... again with a substantial expense to the donor(s) of converting these publications into digital form. Surprisingly, you have made this burdensome and expensive request without any offer to reimburse the charitable donor(s) for their time and expense to do all of this work for you. In your final statement you then wistfully hope that some benevolent vendor should raise their hand and offer you a (free) copy and then insult that same vendor buy stating that you won't hold your breath for that. My Second Question: Are you crazy? From your signature address; it appears that you are employed by a company called Halozyme Therapeutics. A quick web search informs us that Halozyme is a for-profit California company that had revenues in the tens of millions in 2008. It was also exciting to learn that Halozyme is in Phase 2 clinical trials of their innovative insulin-PH20 with individuals that suffer from Type 1 diabetes. Very impressive. Here is some good news for you. I happen to have ALL the operator manuals for the equipment that you are requesting. I will copy them for you and send them to you at my expense under one condition. You state that if someone can do this for you that you will repay the favor, if at all possible. I have a family member that is a Type 1 insulin-dependent diabetic. All I ask in return is that you arrange with your marketing department that they send me a one-year's supply of PH20 when it is released to the public... all at no charge, including shipping handling of course. My Third Question: Do we have a deal? I look forward to your reply, ~ Ford Ford the demon vendor Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. (a for-profit corporation just like yours) Golden Valley, MN Hello Everyone. I our lab we've purchased all used equipment. None of these instruments came with a user's manual. I am in need of a manual (hard-copy or PDF) for the following: Sakura Tissue-Tek VIP 3000 Leica Jung Histo Embedder Microm HM335E Microtome I do realize that requesting a copy of these is a lot to ask of someone. It takes a lot of time to copy a 50-page manual. I'll repay the favor, if at all possible. I'm hoping that a vendor may raise their hand and offer a copy? I won't hold my breath for that... Thanks everyone. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com mailto:jander...@halozyme.com The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error,
RE: [Histonet] Paraffin Block trimming
Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
We have used a variety of cassettes and paraffin over the years...same result. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 11:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http
RE: [Histonet] Paraffin Block triming
I also use mine warm Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, March 19, 2009 1:03 PM To: 'Doug Showers'; Histonet Subject: RE: [Histonet] Paraffin Block triming I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds cold at room temperature and don't have much leakage whereas techs who keep the molds hot have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged
RE: [Histonet] Paraffin Block triming
I use the minimum amount in the base mold then top it off after I add the cassette top. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, March 19, 2009 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Paraffin Block triming I think, it depends on the amount of paraffin you fill in the mold, before you put the cassette on it. If it is a little too much, you press the paraffin out. Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] metal molds vs. disposable molds
I agree. I also find you get a more level plane which makes trimming easier. We use both in our group and the individuals that use the disposable tend to use them multiple times before tossing them. I have not had to clean the metal ones I use in years but have heard you can toss them in the clean cycle of your tissue processor. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 10, 2009 12:00 PM To: histonet@lists.utsouthwestern.edu; Sharon Campbell Subject: Re: [Histonet] metal molds vs. disposable molds Metals molds are easier to work with and almost indestructible, but if you have money to throw to the air and patient and time in large supply, use plastic. René J. --- On Tue, 3/10/09, Sharon Campbell shar...@celligent.net wrote: From: Sharon Campbell shar...@celligent.net Subject: [Histonet] metal molds vs. disposable molds To: histonet@lists.utsouthwestern.edu Date: Tuesday, March 10, 2009, 11:55 AM Hello everyone, We have a debate going on about purchasing metal molds vs. disposable plastic molds. Which is better? Is the cost better in the long run to buy metal? Also, how does everyone clean their metal molds? Thanks Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 800-524-6779 ext. 104 704-970-3304 Direct Line shar...@celligent.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Express
We have had no negative impact on either. We did many parallel runs initially and had to make no modifications. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Friday, February 20, 2009 10:12 AM To: Histonet Subject: [Histonet] Sakura Express Good Morning All - Our facility is looking to purchase a Sakura Express Tissue Processor and would like any feedback from other users as to how this affected molecular testing, as well as IHC. Were there any needed changes that needed to take place after implemented this processor? Mary Abosso ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microwave processors
We have the Sakura Xpress and really like it. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Thursday, February 12, 2009 1:19 PM To: histonet Subject: [Histonet] Microwave processors Hi all, What experiences have you all had with microwave processors? Sakura? Milestone? We're trying to figure something our. I know there will be some passionate responses...And I look forward to them! Thanks so much, Erin Martin, HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: uncertified techs in Histology
Jodie, I agree, but a good program cannot be beat. I had 2 years of college back in the early 1970's and then attended a 12 month accredited histology program. I passed my registry and have been in this field ever since. However, it was not until I went back and complete my degree (just a few years ago) that I was eligible for the level of pay that my co-workers that had degrees were already earning. I can honestly say that those extra 2 years of college did not make me a better employee but it did bring a great since of accomplishment and pride. The 12 months of training in the accredited program I attended is what made be an accomplished histotech. Jeanine Bartlett From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jodie Robertson Sent: Wed 2/11/2009 5:26 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: uncertified techs in Histology I've been listening to this and there's so much controversy. The real bottom line of this is work ethics. It really doesn't matter if you have a degree or not. You have to work, and work hard to prove yourself. I'm proud to be OJT. I worked hard for my certification. I didn't have the theory behind things initially but I learned them thru mentoring and reading and studying and such. Some people have good work ethics and are stellar performers and others don't and some of those that don't have a degree and some don't. I've been doing this for 15 years now and I've worked with both types. I've had wonderful opportunities and have taken advantage of each and every one to learn more. I learn every day. Histology is a wonderful constantly changing field in many ways. Instead of stating negatives so much, look how far we've come and how far we can go. Jodie Robertson, HT(ASCP) QIHC Pathology Sciences Medical Group Chico, CA 95926 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Wednesday, February 11, 2009 1:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: uncertified techs in Histology I've been a research scientist and laboratory manager for over 20 years, and I learned histology by on-the-job training. Apparently I'm pretty good at it, and the people I've trained became good at it. I am slightly bothered by the fact that after all these years of experience, I'm still not considered a real histologist. Mind you, I understand that histology for diagnosing human patients ought to be more rigorous than histology for pure research. I have actually hired on certified histotechnologist, and he was the best I ever had. I mean, he was even as good as me. Jerry Ricks Research Scientist University of Washington Department of Pathology From: pmar...@vet.upenn.edu To: sjchta...@yahoo.com; Histonet@lists.utsouthwestern.edu Date: Wed, 11 Feb 2009 10:21:52 -0500 Subject: RE: [Histonet] uncertified techs in Histology CC: It is called be cheap and don't pay for training!! Sorry that is how I see and ASCP does not seem to care or force the issue for training in histology. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Wednesday, February 11, 2009 9:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] uncertified techs in Histology Any thoughts or experiences with my fellow HT/HTL's(ASCP). What the big advantage do all these facilities think there gaining by going with unregistered techs, especially when theres always ongoing quality issues when theres so many trained certified HT looking for work? In my area of the country I can't believe how many Hospitals go this way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows Live(tm): E-mail. Chat. Share. Get more ways to connect. http://windowslive.com/howitworks?ocid=TXT_TAGLM_WL_t2_allup_howitworks_ 022009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] Sakura Glas Coverslipper and Xylene Sub
I found this in the Operating Manual Compatible Solvent Xylene D-Limonene-based substitutes Aliphatic-Hydrocarbon-based substitutes Compatible Mounting Medium Xylene-based mounting medium D-Limonene-based mounting medium Aliphatic-Hydrocarbon-based mounting medium Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, February 10, 2009 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Glas Coverslipper and Xylene Sub Hello, We use Xylene in the Sakura Glas auto-coverslipper and I would like to know if anyone uses a xylene sub in this piece of equipment. I'm looking for one that is okay to use in the equipment and one with less odor than Xylene. Thanks in advance, Paula Lucas Lab Manager BioPath MG ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Who can gross??
Hey, I am one of those short people and I do NOT have tiny little teeth OR nasty little feet. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, January 30, 2009 1:49 PM To: 'histonet' Subject: RE: [Histonet] Who can gross?? I hear you Judy... you have to pick them up just to say hello! ;-) (Now before there is a flame of political correctness coming back at us... everyone lighten up. I AM one of those short people, and I can make fun of myself if I want too). ;-) Ford M. Royer, MT(ASCP) ...who use to have to sit on a phone book so that I could reach the microscope to scan slides for QA. Minnesota Medical, Inc. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams Sent: Friday, January 30, 2009 11:59 AM To: Charles.Embrey Cc: histonet Subject: RE: [Histonet] Who can gross?? Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: As short histo techs what kind of aids are you using, stepladders or stools? Sorry, I couldn't resist. There is a ton of information on the histonet archive about this. A search should yield all the info you seek and more. Charles Embrey, PA(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Friday, January 30, 2009 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can gross?? Hi All, We are short histo techs and are using aids to help more and more. Our manager believes they can be doing the grossing of a histo tech as long as they have a tech or Dr to consult with. Can someone remind me of the guide lines for grossing. One has a BSc, is that enough? Thanks in advanceSheila Adey HT MLT Port Huron Hospital Michigan _ Windows Live Messenger. Multitasking at its finest. http://www.microsoft.com/windows/windowslive/messenger.aspx___ __ __ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sectioning on different microtomes
I simply align my block holder to match up with the block. We get many outside blocks so I have learned to be pretty good at this. From: histonet-boun...@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Sat 1/24/2009 7:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Prion Contamination
All, The formic acid is a post-fixation step. http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s7d.htm#Table%205.%20Tissue %20preparation Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, January 22, 2009 6:46 PM To: sal...@exchange.hsc.mb.ca; Histonet Subject: RE: [Histonet] RE: Prion Contamination I just researched this recently for procedure writing. I referenced an article from the CDC that made mention of specific handling guidelines for histology, and also specifically the formic acid treatment step. I do not have the article in front of me, but by my recollection I believe that it stated that the formic acid was a post-fixation step. I found this article relatively easily by using the search field for CJD from the CDC website. You can check my recollection here: http://www.cdc.gov/od/ohs/biosfty/bmbl5/sections/SectionVIIIH-PrionDisea ses.pdf Thanks Joelle Weaver HTL(ASCP) Date: Thu, 22 Jan 2009 13:21:25 -0600 From: sal...@exchange.hsc.mb.ca To: tbr...@holyredeemer.com Subject: [Histonet] RE: Prion Contamination CC: histonet@lists.utsouthwestern.edu Hi, I was reading your email of Jan. 15/09 on the subject of Prion Contamination. In it you had made the following statement, which I was curious as to where this information had come from. the procedure to inactivate using formic acid is followed before fixation and processing. If the tissue is fixed (formalin or other common fixatives) then you would be actually fixing the prion's ability to NOT be inactivated. I had read again the WHO Guidelines on CJD that you had a link to could find no mention of the fact that the formic acid step for de-activating the prions had to be done before any fixation. The WHO instructions: P. 18 8.2.2 Histopathological examination: states formic acid treatment consists of placing small pieces of fixed tissue, no more than 4 to 5 mm thick, in 50 to 100 ml of 95% formic acid for an hour. I have been dealing with CJD brains for many years always following the CDC, WHO Health Canada guidelines but have never read any studies that had indicated that the fixing in formalin made the treatment in formic acid ineffective. If you have any further information I would really appreciate seeing it. Thank you S. Allen sal...@hsc.mb.ca _ Windows Live(tm): E-mail. Chat. Share. Get more ways to connect. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_allup_explore_012009 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prion contaminated tissue processing
It will not. Check out the CDC and/or the WHO guidelines. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Thursday, January 15, 2009 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prion contaminated tissue processing Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prion contaminated tissue processing
Please read the protocols at the CDC and/or the WHO website. Bleach in not effective. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, January 15, 2009 12:40 PM To: rjbu...@yahoo.com Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] prion contaminated tissue processing Viable prions have been found in 20 year old paraffin blocks.Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbu...@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin har...@oncology.wisc.edu cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. René J. --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PRO Cycler BR instrument
We have 2 and we have service agreements on them. We have no problems getting our PMs done. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R Sent: Wednesday, January 14, 2009 10:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] PRO Cycler BR instrument Hi Netters, We have a Procycler by BR Instrument we have been calling the company with some questions and they refuse to send someone out to PM the recycler. We don't have major problems, but sometimes it doesn't want to read the temp probe, and we've had some issues that are 'solved' by shutting the machine totally off ... when we start it again, 5 minutes later, it seems to be fine. The representative we speak with wants to give us information over the phone to 'fix' the machine, but we are wondering if someone could come in that we could actually talk to and get some further PM done on it. Does anyone else use this recycler and/or have you had a tech come to service your machine? If you did, what did you bribe them with? Thank you so much for the replies to my earlier question about outside labs. WOW I got the responses to that question! Thanks again so very much! Hope everyone is having a great day... Amy HT, Holy Spirit, Camp Hill PA Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processing 3-dimensional cell cultures into paraffin
Histogel works great and does not absorb water like plain agar does. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Tuesday, January 13, 2009 12:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processing 3-dimensional cell cultures into paraffin I have processed and embedded cells grown in/on agar, or post-embedded in agar or agarose, many times. It really isn't much different from processing and embedding tissue. You can trim the agar blocks to appropriate size with a scalpel (if necessary) either before or after fixation (if you trim them before fixation, do it while they are cold, so the agar is firm), and place them in a cassette just like a piece of tissue. Put them on the processor as usual, processing times the same as you would use for tissues of equivalent size, and embed as usual. One difference in sectioning however. Don't place the blocks in water after facing them off. The agar absorbs too much water and becomes soft. The blocks should be cut cold, but not moistened. Either place them on a cold plate without water, or put them in the refrigerator after facing, and take them out one at a time as you section them. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome Cutting Safety
I use my fingers too, so no help there. But an automated microtome is great because it frees both hands to handle a ribbon as it is coming off the blade. It is easy to get careless if you use a foot pedal but the one time I have been seriously cut it was with a manual microtome so I guess the moral is to simply be careful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna Harclerode Sent: Tuesday, January 06, 2009 11:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Cutting Safety Begin forwarded message: From: Donna Harclerode erid...@cox.net Date: January 6, 2009 8:27:14 PM PST To: histonet-requ...@lists.utsouthwestern.edu Subject: Microtome Cutting Safety Anyone know of a paraffin microtome that you can section WITH the knife guard in position? I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this . I figured if anyone knows they would be on this list. I tried the Leica RM2255 (both in automated and manual mode) and I could sort of section a couple small blocks with the guard up, but it was not going to work with anything except perfect processed small blocks and not well for those. Any opinions on the safety automated versus manual microtomes? I adore automated cryostats (Leica 3050 is my favorite) , but I can not figure why use an automated paraffin microtome. Logically I always figured an automated can do more damage, but really have no facts. Thanks in advance, Donna Harclerode, HT, HTL, SLS, (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome Cutting Safety
I guess I wasn't totally clear in my earlier reply. I use my bare fingers but I also use a camel hair brush as Beth states. I direct the brush with my right hand and pick up the first part of the ribbon with my left. The brush is used to hold the last part of the ribbon to aid in maneuvering to the waterbath. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Beth Millerman Sent: Wed 1/7/2009 4:53 PM To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Cutting Safety I use a fine tipped camel hair brush (sometimes wetted water) to ease the ribbon from the blade. It works great without any danger to the operator. The most that can happen is losing the end of the brush and ending up with a stick. It also prevents damaging the blade holder. On convincing your techs...it could help them develop fine motor movement/cordination if they truely want to develop their professional expertise. Beth Millerman, HT, SRA/SWC Stiefel Laboratories, Inc JR R rosenfeld...@hotmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu Wed 07 Jan 2009 11:30 AM --- To histonet @lists.ut southwest ern.edu cc Subject [Histonet ] Microtome Cutting Safety --- I have always used forceps to keep my fingers safe, but some techs are harder to convince to do this... Dear God in Heaven. Not only are Dumont forceps are more precise and delicate than fingers, but forceps don't bleed. I would never, ever allow anyone in my lab to use their fingers to pull ribbons off of the microtome. I may as well allow them to pipette concentrated HCl by mouth. Write S.O.P's. Then follow them and enforce them. Jerry Ricks Research Scientist University of Washington Department of Pathology Date: Wed, 7 Jan 2009 07:25:32 -0500 From: j...@cdc.gov To: erid...@cox.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome Cutting Safety CC: I use my fingers too, so no help there. But an automated microtome is great because it frees both hands to handle a ribbon as it is coming off the blade. It is easy to get careless if you use a foot pedal but the one time I have been seriously cut it was with a manual microtome so I guess the moral is to simply be careful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna Harclerode Sent: Tuesday, January 06, 2009 11:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Cutting Safety Begin forwarded message: From: Donna Harclerode erid...@cox.net Date: January 6, 2009 8:27:14 PM PST To: histonet-requ...@lists.utsouthwestern.edu Subject: Microtome Cutting Safety Anyone know of a paraffin microtome that you can section WITH the knife guard in position? I have always used forceps to keep
RE: [Histonet] Slide drying
I would suggest filing using the spacer coils and then removing them after 4-7 days. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, December 22, 2008 2:06 PM To: histonet Subject: [Histonet] Slide drying Hello everyone, I was asked to find out how to dry slides quickly. They are glass coverslipped in an automated coverslipper at the reference lab we use and the our docs want them filed in less than 12 hours from the time they are coverslipped. We have been putting them in a 125 degree C convection oven for a few hours but the slides still get all stuck together in the file. They will not consider film coverslipping. Does anyone else file this quickly? I am grateful for any suggestions! Erin Martin UCSF Dermatopathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] dako artisan gram and afb
We have not experienced this problem, thank goodness! Have you tried different reagent packs? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carrie Disbrow Sent: Thursday, December 18, 2008 8:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dako artisan gram and afb Happy Holidays to all. W ea re using the Dako Artisan stainer and have had problems lately with the AFB and Gram staining inconsistently from day to day. We have had the machine services and the PM done and the problem has returned. Has anyone had the same problem and what did you do? Thanks for all your help. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Casual Histotech
Who knew? Thanks! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Laurie Popp Sent: Wednesday, December 03, 2008 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Casual Histotech Casual Histotech is usually considered to either be very part time or a pool position to cover only when needed. Laurie Popp, BA HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Casual Histotech
As opposed to the rest of us...you know, the formal ones. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of JD Sent: Tuesday, December 02, 2008 10:42 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Casual Histotech My thoughts exactly. Casual histotech?___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question
LOL! That is too funny! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cheryl Sent: Tuesday, November 18, 2008 1:17 PM To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu; 'Andrea Grantham' Subject: Check out this MSDS exerpt for HistoClear RE: [Histonet] HistoClear question Read down to Characteristics-- I want to know who they got to taste this stuff??!!! Cheryl _ HISTOCLEAR (C10H16) A naturally occurring hydrocarbon found in plants. SYNONYMS 1-methyl-4(1-methylethenyl) cyclohexane p-mentha-1,8-diene, d-limonene, Safsolv, Histolene, dipentene. GENERAL PRECAUTIONS Avoid skin contact, can cause dermatitis. Avoid inhalation. Keep away from heat or naked flames. Wash hands thoroughly after handling. CHARACTERISTICS Clear liquid of low viscosity with a fresh, light and sweet citrusy odour. The taste is sweet, refreshing, citrus-like and mild. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RA Lamb has been sold to the evil empire
Crap. So much for the hospitalities. From: [EMAIL PROTECTED] on behalf of Joe Nocito Sent: Wed 11/12/2008 5:17 PM To: Bernice Frederick; 'Jack Cates'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RA Lamb has been sold to the evil empire great. Soon there will be only one booth at the states and the NSH conventions - Original Message - From: Bernice Frederick [EMAIL PROTECTED] To: 'Jack Cates' [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 12, 2008 2:46 PM Subject: RE: [Histonet] RA Lamb has been sold to the evil empire I keep telling everyone it's going to be histosupplies are us one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jack Cates Sent: Wednesday, November 12, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RA Lamb has been sold to the evil empire I am very sorry to see this happen again to a great company who bent over backwards to assist customers. I hope ThemoShandonFisherRichardRaymond (to be continued) will start to keep the parts that made Richard Allen, Raymond A. Lamb and others great to work with I am also very sorry to see Tonia and Jerry go. Tuesday, Nov. 11, 2008 Thermo Fisher Scientific Strengthens Anatomical Pathology Portfolio with Acquisition of Raymond A. Lamb WALTHAM, Mass. - Thermo Fisher Scientific Inc., the world leader in serving science, today announced it has acquired Raymond A. Lamb Ltd., a manufacturer of histology and anatomical pathology products based in Eastbourne, U.K., near London. In business since 1964, Raymond A. Lamb has grown to become a global provider of products for the pathology laboratory. Recently, its focus has been on systems that automate the labeling and tracking of slides and cassettes that carry patient tissue samples, reducing the possibility of errors during their processing, storage and retrieval. The company has had a longstanding relationship with the anatomical pathology business of Thermo Fisher as a supplier of labeling products for cassettes and glass slides, as well as other anatomical pathology equipment. Our anatomical pathology customers - in both clinical and research markets - are demanding better solutions for tracking the increasing number of samples that they need to process and diagnose, said Marijn E. Dekkers, president and chief executive officer of Thermo Fisher Scientific. This acquisition brings new-generation systems that can be integrated with our existing portfolio of anatomical pathology equipment and consumables to create a more efficient, and reliable, workflow. Raymond A. Lamb had revenues of approximately $9 million in 2007, and will be integrated into Thermo Fisher's Analytical Technologies Segment. About Thermo Fisher Scientific Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com. Advertisement The following constitutes a Safe Harbor statement under the Private Securities Litigation Reform Act of 1995: This press release contains forward-looking statements that involve a number of risks and uncertainties. Important factors that could cause actual results to differ materially from those indicated by such forward-looking statements are set forth in the company's Quarterly Report on Form 10-Q for the quarter ended September 27, 2008, under the caption Risk Factors, which is on file with the Securities and Exchange Commission (SEC) and available in the Investors section of our Website under the heading SEC Filings. Important factors that could
RE: [Histonet] 200 proof alcohol?
I like mine with blue-cheese olives but to each his/her own. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Joe Nocito Sent: Wednesday, November 05, 2008 7:23 PM To: Caroline Bass; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] 200 proof alcohol? 200 proof is 200 proof, can't get any purer than that. Of course, I would take the taste test first. Green olives stuffed with Feta cheese goes good. JTT - Original Message - From: Caroline Bass [EMAIL PROTECTED] To: histonet@lists.utsouthwestern.edu Sent: Wednesday, November 05, 2008 11:15 AM Subject: [Histonet] 200 proof alcohol? Hello, I¹m trying to buy some alcohol for both histology and molecular biology protocols. But I¹m having some difficult getting ³molecular biology grade² 100% ethanol. The hospital I work at sells 200 proof ethanol, but can¹t say anything about the quality. Since it¹s 200 proof, is that basically the same as molecular biology grade? How about histology grade? I seem to recall using hospital provided ethanol for molecular biology experiments in my postdoc, so I think it should be ok. Any advice is appreciated! Caroline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] President
Thank you Angela! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Angela Bitting Sent: Thursday, November 06, 2008 10:16 AM To: histonet@lists.utsouthwestern.edu; Joachim Hehl Subject: Re: [Histonet] President not really...just want to keep this arena of scientific thinking sacred (and totally non-partisan)! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Joachim Hehl [EMAIL PROTECTED] 11/5/2008 10:58 AM Interesting, the histonet list seems to be occupied by ultraconservatives. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] funding for NIH
All I will say on the matter is that if more funding is approved I hope it is simply pulled from somewhere else and is not in addition to.because that would mean more taxes! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Mark Tarango Sent: Wednesday, November 05, 2008 12:17 PM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] funding for NIH I definately hope that now that Obama is going to be President that the reseach will get moving again. He's got The Congress too. P.S. I hope this message doesn't shut the histonet down. Mark On 11/5/08, Geoff McAuliffe [EMAIL PROTECTED] wrote: Hi Emily et al. The President, any President, can put $ for NIH is the budget request he/she submits to Congress but ONLY Congress can appropriate money. That is in the Constitution. Several of my colleagues are out of work due to loss of grant support 'cause NIH, while not broke, has many worthy projects to fund and cannot fund them all. Geoff Emily Sours wrote: Isn't the election over? Griping will do you no good, spambot! So, being in grant funded research, I hope our new president will put some money back into NIH. (I was hoping that no matter who won, so my political views will not spam you) Anyone else feel that way? Emily -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 [EMAIL PROTECTED] ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] funding for NIH
unfortunately, all government funding comes from us peons...that's the way it has always been and will always be. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Peter Carroll Sent: Wednesday, November 05, 2008 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] funding for NIH because that would mean more taxes! Making more than $250,000/year doing histology? Where do I sign up?! ;) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Be careful, be very careful when you Vote!
Good one! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Angela Bitting Sent: Tuesday, November 04, 2008 11:15 AM To: 'Sam Histology'; Allen Smith Cc: 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Be careful, be very careful when you Vote! I'd say we all should be more worried about Revelations Smith, Allen [EMAIL PROTECTED] 11/4/2008 10:06 AM Sam Histology seems to be unfamiliar with Exodus 23:1. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Sam Histology Sent: Tuesday, November 04, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Be careful, be very careful when you Vote! Barack Hussein Obama was born in Honolulu, Hawaii, to Barack Hussein Obama Sr. (black muslim) of Nyangoma-Kogelo, Siaya District, Kenya, and Ann Dunham of Wichita, Kansas. (white atheist ). When Obama was two years old, his parents divorced and his father returned to Kenya. His mother married Lolo Soetoro -- a Muslim -- moving to Jakarta with Obama when he was six years old. Within six months he had learned to speak the Indonesian language. Obama spent two years in a Muslim school, then two more in a Catholic school in Jakarta. Obama takes great care to conceal the fact that he is a Muslim while admitting that he was once a Muslim, mitigating that damning information by saying that, for two years, he also attended a Catholic school. Obama's father, Barack Hussein Obama, Sr. was a radical Muslim who migrated from Kenya to Jakarta, Indonesia. He met Obama's mother, Ann Dunham-a white atheist from Wichita, Kansas-at the University of Hawaii at Manoa. Obama, Sr. and Dunham divorced when Barack, Jr. was two. Obama's spinmeisters are now attempting to make it appear that Obama's introduction to Islam came from his father and that influence was temporary at best. In reality, the senior Obama returned to Kenya immediately following the divorce and never again had any direct influence over his son's education. Dunham married another Muslim, Lolo Soetoro who educated his stepson as a good Muslim by enrolling him in one of Jakarta's Wahabbi schools. Wahabbism is the radical teaching that created the Muslim terrorists who are now waging Jihad on the industrialized world. Since it is politically expedient to be a Christian when you are seeking political office in the United States, Obama joined the United Church of Christ to help purge any notion that he is still a Muslim. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Presidential Voting Infomation
I received something similar but it was pro-Obama/anti-McCain on another listserv. I guess both parties are guilty. From: [EMAIL PROTECTED] on behalf of Greg Dobbin Sent: Mon 11/3/2008 11:40 AM To: histonet@lists.utsouthwestern.edu; [EMAIL PROTECTED]; [EMAIL PROTECTED] Subject: RE: [Histonet] Presidential Voting Infomation I don't remember seeing this persons name on the Histonet before. Could he be a Republican Party volunteer who joins various listservs for the sole purpose of garnering support for Mr. McCain?? It certainly would not surprise me. The fact that so many of us have read it already tells me he's accomplished his mission! Greg Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 And in the end it's not the years in your life that count. It's the life in your years. - Abraham Lincoln - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue swelling out of block
Is he saying that he embeds processed tissue and removes the chilled paraffin block from the base mold that it then swells up in the paraffin? Before it is trimmed and placed on ice? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 [EMAIL PROTECTED] -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Wednesday, October 01, 2008 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue swelling out of block Hi all, a friend recently got a job in a veterinary histo and has some troubles in cutting. He tells about paraffinblocks, where the tissue is swelling out while warming like a small hill. I referred to the possibilty of taking up humidity that causes swelling. Do you know other causes for this problem? Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
