Re: [Histonet] [External] Expiration date after opeing a reagent

[Bitting, Angela K. via Histonet]

This sounds absurd to me.

-Original Message-
From: Paula via Histonet 
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent

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Hello,



We just got CLIA inspected and had no deficiencies. The inspector did say he 
wants to see us write down on any reagent containers when it was opened, and 
write down the new expiration date.



The containers have the expiration date on them from the manufacturer, but the 
inspector said when the container is opened, the expiration will change and we 
need to write the date on the container under the opened date.



I've never heard of this requirement and I would like to ask if you have.
He said if the vendor does not give us any written documentation of when a 
reagent would expire after it's opened, then we should seek an alternate vendor 
who can.



Any comments, etc. are greatly appreciated.



Paula Lucas

Bio-Path Medical Group



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Re: [Histonet] [External] Re: Coffee at the desk

[Bitting, Angela K. via Histonet]

Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the 
VIP without fear of admonishment.

-Original Message-
From: Jay Lundgren via Histonet 
Sent: Friday, June 2, 2023 3:34 PM
To: Piche, Jessica 
Cc: Histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] Coffee at the desk

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Next thing you're going to be telling me it's not OK to embed with a 1 foot 
tall bunsen burner open flame and a Coke on the cold tray!

On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> It also doesn't set a good impression for everyone else in the lab
> since they can't have a coffee at their stations. If it could be done,
> I wouldn't want everyone else's coffees on my desk either,  but I'd
> have to because I'd want to make it fair to everyone. If only it was safe for 
> all of us
>
> Have a great weekend everyone!
>
> Jessica
>
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory Histology Team Leader
> 203-573-7167
> 
> From: McLaughlin, Stacy L. via Histonet
>  >
> Sent: Friday, June 2, 2023 6:09 AM
> To: Hannen, Valerie ;
> Histonet@lists.utsouthwestern.edu 
> Subject: Re: [Histonet] Coffee at the desk
>
> [EXTERNAL MSG]
>
> It is not allowed.  The area you want food/drink has to be completely
> separate- floor to ceiling walls and a door.
>
> Stacy McLaughlin, HT(ASCP) QLScm
> Histology Supervisor
> Cooley Dickinson Hospital
> 30 Locust Street
> Northampton, MA 01060
> Office:  (413)582-2019
> Lab:  (413)582-2179
> smclaughl...@cooleydickinson.org
>
>
>
>
> -Original Message-
> From: Hannen, Valerie via Histonet 
> Sent: Thursday, June 1, 2023 3:40 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Coffee at the desk
>
> External Email - Use Caution
>
> Is there any regulation/ reason why a Histology supervisor whose desk
> is in the department and the desk area is taped off and designated as
> a "clean area (  meaning no chemicals or specimens cross the taped off
> area) can't have a closed coffee cup at their desk??  We are having a
> debate in our Lab and I wanted to get a consensus.
>
> Thanks in Advance,
>
> Valerie
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish
> Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333  Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
>
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Re: [Histonet] [External] Clarification about Immunohistochemistry

[Bitting, Angela K. via Histonet]

We use an Azure B counterstain. Melanin has a greenish tinge after 
counterstaining.

-Original Message-
From: jayalakshmy p.s via Histonet  
Sent: Monday, April 11, 2022 11:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Clarification about Immunohistochemistry

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hai all Histonetters
 Please anybody clarify my this doubt if possible.
When doing Immunohistochemistry for confirmation of Melanoma with DAB 
chromogen(brown color)the interpretation is not possible because of obscuring 
by the dense melanin pigment. We dont have any other color chromogen. I tried 
ihc after bleaching but the section gets detached even from charged slides. Is 
there any other effective way to do this?
Thanks in advance
Regards
Dr. P S Jayalakshmy
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Re: [Histonet] [External] Re: Another Dispenser Failure

[Bitting, Angela K. via Histonet]

Is there any other way! :-O

Sent from my iPhone

> On Feb 28, 2019, at 3:30 PM, Mark Tarango via Histonet 
>  wrote:
> 
> I hope everyone is using on-slide controls :-)
> 
> On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Another one!!!
>> Our Her2 antibody dispenser failed, LOT #E22628
>> This one "supposedly" FDA approved.
>> Roche, why do you continue to lie to consumers of your product?  You claim
>> you've "fixed" the problem but your Ventana dispensers DON'T WORK!
>> This is patient care!  Why don't you care about the customers and patients
>> you are supposed to be serving
>> Shame on you.
>> 
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> 
>> -Original Message-
>> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
>> histonet-requ...@lists.utsouthwestern.edu]
>> Sent: Thursday, February 28, 2019 1:00 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: Histonet Digest, Vol 183, Issue 23
>> 
>> 
>> Today's Topics:
>>   1. H Staining question (Charles Riley)
>>   2. Re: H Staining question (Jay Lundgren)
>>   3. FYI- Roche Ventana users (Cassie P. Davis)
>> Message: 3
>> Date: Thu, 28 Feb 2019 15:23:16 +
>> From: "Cassie P. Davis" 
>> To: histonet 
>> Subject: [Histonet] FYI- Roche Ventana users
>> Message-ID: 
>> Content-Type: text/plain; charset="iso-8859-1"
>> 
>> Hi Histoland,
>>I am biting my tongue HARD and just letting you know so it doesn't
>> happend to you. I just got off the phone with Roche here is the heads-up.
>> If one of their anitbody dispensers fails DO NOT put the antibody in one
>> of their prep kits, as soon as you do they consider it off label use.
>> Call customer service immediately and have them overnight a replacement!
>> Cassandra Davis
>> Histology Technician
>> AP Laboratory
>> 302-575-8095
>> Email:  cda...@che-east.org
>> 
>> 
>> 
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>> 
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[Histonet] NOTCH2 help

[Bitting, Angela K. via Histonet]

Is anyone running NOTCH2 on Bond III OR Benchmark Ultra? Can I take a peek at 
your protocol and antibody selection?


Sent from my iPhone

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Re: [Histonet] [External] Re: Automated Slide Stainers

[Bitting, Angela K. via Histonet]

There are slide basket inserts to accommodate that sized slide for Sakura 
stainers. The DRS2000 for example. Check out this web page.

http://www.tedpella.com/histo_html/slides-large.htm


-Original Message-
From: Atoska Gentry via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, August 05, 2016 11:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] Automated Slide Stainers

Hello, one of my department's researchers is in need of an automated slide 
stainer which accommodates 3"x2" microscope slides. If you have experience with 
such will you please contact me at your earliest convenience? Regards, Atoska 
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Re: [Histonet] [External] Crystals in PAS from Ventana

[Bitting, Angela K. via Histonet]

Hi Mark,
 We still have this issue after two years of complaining to Roche.  We've had 
multiple TAS's come in and we've sent slides to Tucson. They've gone over the 
instruments, etc. Long story short, their fix was adding something to their 
package insert that says "Schiff's Reagent is known to contain some needle-like 
precipitate. At expected levels, this precipitate should not affect assay 
performance". Good luck.

-Original Message-
From: Mark, Danielle via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, July 20, 2016 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Crystals in PAS from Ventana

I am wondering if anyone else has been having issues with the PAS stain.  The 
crystals are not rinsing off of the slide using the benchmark special stainer.  
We are on our 3rd lot number, no change.  We have had our tubing changed and 
our instrument checked to make sure the rinse is appropriate.  This problem is 
causing our lab great frustration.  Any feedback would be appreciated.


Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories
8901 W Lincoln Ave. | West Allis, WI 53227
O: 414.328.6235 | F: 414.328.6237 |
danielle.m...@aurora.org

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[Histonet] curiosity may kill me

[Bitting, Angela K. via Histonet]

I'm curious as to why we heat the Auramine Rhodamine when we are staining 
tissue sections but our Micro lab stains at room temp.  It is because "it's 
always been done that way"?
Thanks group,
Angie






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Re: [Histonet] Histonet Digest, Vol 150, Issue 2

[Bitting, Angela K. via Histonet]

This seems to be the trend of society in general these days.
No manners.

-Original Message-
From: Hudson, Pamela R ASHVAMC via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, May 04, 2016 7:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2

OMG  This is terrible.  I can't believe I belong to a group of such 
unprofessional people.

Pamela R. Hudson, HT (ASCP)
Charles George VA Medical Center
Pathology Department
1100 Tunnel Road
Asheville, NC   28805
828-298-7911   x5747
pamela.hud...@va.gov

-Original Message-
From: T H via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 03, 2016 4:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2

Good to know that I am not the only on that think Rene is negative to others.  
Hey Rene it's not just one person that thinks your a dirt bag.


From: histonet-requ...@lists.utsouthwestern.edu

Sent: Tuesday, May 3, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2

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Today's Topics:

   1. No More Blog Posts --  Over and Out! (Lester Raff MD)
   2. IHC positive controls (Cindy Bulmer)
   3. Re: No More Blog Posts --  Over and Out! (Rene J Buesa)
   4. Fwd: (Linda Margraf)
   5. Re: No More Blog Posts --  Over and Out! (Manfre, Philip)
   6. Re: IHC positive controls (Morken, Timothy)
   7. Re: No More Blog Posts --  Over and Out! (Boyd, Debbie M)
   8. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
   9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
  10. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
  11. Re: Fwd: (Rene J Buesa)
  12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
  13. PAS/Decal Question (Marcum, Pamela A)


--

Message: 1
Date: Mon, 2 May 2016 17:39:28 +
From: Lester Raff MD 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
 
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local>

Content-Type: text/plain; charset="us-ascii"

To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a
line at les.r...@post.com   The mailing list
is never used for any purpose other than announcing a new blog post. Be sure to 
let me know you are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203



--

Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8


Hello Histoland,

I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?

1)  Cut (serial sections, stain the last slide for bugs) and oven time
(60) for 1 hr.
 then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time and put 
slides
 directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.

Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX






--

Message: 3
Date: Mon, 2 May 2016 18:22:59 + (UTC)
From: Rene J Buesa 
To: Lester Raff MD ,
"'histonet@lists.utsouthwestern.edu'"

[Histonet] Auramine Rhodamine staining

[Bitting, Angela K. via Histonet]

Hi Histonet Colleagues,

I'm surveying Histology labs about Auramine-Rhodamine staining for acid fast 
organisms.
If you'd like to help me by participating in my survey please follow the link 
below.
Thank you in advance for your responses.

https://www.surveymonkey.com/r/7JPL5K2


Angie

Angela K. Bitting, HT(ASCP),QIHC
Analytical Specialist, Histology
Geisinger Health System
100 N. Academy Avenue
Danville, PA 17822-1920
t. 570-214-9634
f. 570-271-5916



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[Histonet] BCL-10 for IHC-P

[Bitting, Angela K. via Histonet]

Has anyone been able to optimize an antibody to BCL-10 using Ventana's 
Ultraview detection?


Angie



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[Histonet] NHPD

[Bitting, Angela K. via Histonet]

Is it me or does anyone else think $20 is a lot of money for a T-shirt?  The 
Histotechnology Professionals Day T-shirts on NSH'S website seem over-priced.

Angie




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[Histonet] Phosphorylase stain for muscle

[Bitting, Angela K. via Histonet]

Can anyone tell me what kind of glycogen to use for this? Sigma's website has 
multiple choices. Thanks.

Angie



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[Histonet] Old microtome knives

[Bitting, Angela K.]

For anyone out there still using the old microtome knives that you have to 
sharpen yourself- I have a box full, maybe 20 knives that are free to a good 
home.  Contact me off line if you are interested.

Warmest regards,
Angie

Angela K. Bitting, HT(ASCP),QIHC
Technical Specialist for Special Stains/ IHC/ISH
Geisinger Health System
100 N. Academy Avenue
Danville, PA 17822-2300
t. 570-214-9634
f. 570-271-5916



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[Histonet] IHC repeat rates

[Bitting, Angela K.]

What does this group feel is an acceptable repeat rate for automated IHC 
staining??

Thanks for your responses in advance.

Angie



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[Histonet] TOMO IHC glass slides

[Bitting, Angela K.]

Does anyone have any experience with using  these slides on a Ventana platform?


Thanks in advance,
Angie


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Re: [Histonet] plants in the lab

[Bitting, Angela K.]

So sad bc spider plants THRIVE ON  the fumes!!

-Original Message-
From: Michelle Lamphere [mailto:michelle.lamph...@childrens.com] 
Sent: Sunday, May 31, 2015 8:36 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Our hospital Safety and Infection Control departments have policies in place 
prohibiting any potted plants from being in the hospital, anywhere.  We can 
have them if they are only in water, but the soil presents an infection control 
issue for patients because of potential mildew, mold, spores, etc.


Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamph...@childrens.com
1935 Medical District Drive | B1.06  | Dallas, Texas  75235



Message: 2
Date: Fri, 29 May 2015 14:23:00 -0400
From: Blazek, Linda lbla...@digestivespecialists.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] plants in the lab
Message-ID:

5a2bd13465e061429d6455c8d6b40e391742126...@ibmb7exchange.digestivespecialists.com

Content-Type: text/plain; charset=us-ascii

Happy Friday all!

Does anyone have documentation of the benefit of having plants in the lab?  I 
know this was discusses quite a while ago but I can't find references for it.  
Any help would be appreciated.

Thanks,
Linda


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[Histonet] Ventana dispensers

[Bitting, Angela K.]

Good morning Histonetters,
Is anyone else having issues with their Ventana detection? My dispensers are 
clogging up with rubbery plugs and mis-dispensing. It's not that they sit in 
the refrigerator and don't get used for long periods of time. We are a high 
volume lab and we go through detection very quickly.   The biggest culprit is 
OptiView. I called in to log a complaint and was told to dig in the drop tubes 
with a pipette tip each time I use them. Really?
I've logged the complaint several times now. Just had a TAS out. She told us to 
do the same thing. I don't know but ever since Roche bought them, I get more 
and more dissatisfied.
If you are having issues too, please contact me off line.

Thanks,
Angie



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[Histonet] Visual alert for processors

[Bitting, Angela K.]

Does anyone know of a way to generate a visual alert (hearing impaired device?) 
to notify us when our processors finish?
They are going to be elsewhere in the building and we need to know when they 
finish. Baby monitor is out of the question due to privacy issues.

Sent from my iPhone


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[Histonet] autdialer for Peloris

[Bitting, Angela K.]

Can anyone recommend a third party auto dialer to connect to Peloris?

Thanks,
Angie




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Re: [Histonet] Nuclear Artifact

[Bitting, Angela K.]

We occasionally see this also.

Sent from my iPhone

 On Apr 21, 2015, at 7:59 PM, Sue suetp...@comcast.net wrote:
 
 OMG we are experiencing the same issue. At first it was just GI and now we 
 are seeing it on prostate. One pathologist said it looks like the tissue has 
 been cooked. The only issue is we can have two biopsies right next to one 
 another in the basket one looks good and one looks bad. My director also 
 thinks it is the processors. I had Thermo out and they could find nothing. We 
 changed out all the reagents and the biopsies were fine than two days later 
 we had some bad ones. I know in July Fisher had a formalin recall associated 
 to the mixture of buffer, water and formalin. We thought that might be it but 
 it is now almost a year later and all the bad formalin should be gone. The 
 histotechs say the tissue is crunchy and they are right. I am running a test 
 tonight of a small needle biopsy that I made from a colon. I placed it is 
 straight formaldehyde overnight and am processing it on our biopsy cycle 
 tonight. My director also wanted us to only put three levels on our Thermo, 
 but he wanted the middle level to have empty baskets. I stopped that today 
 because I think the other issue is that the poor biopsies may be on the top 
 level and as the reagents are used the level changes, and also due to 
 displacement with the middle level being empty the reagent levels may not 
 reach the top. We just do not have the manpower to inspect every reagent 
 every day, we have 6 processor and it would take a tech all day. We actually 
 take a digital picture when they come out of the processor. I want to check 
 my problems cases tomorrow. We do not use sponges but the only other like was 
 the PA who was wrapping the blue paper very tight around the tissue. I really 
 do not think this is the issue though.. Any other insight would be greatly 
 appreciated. 
 
 Susan T. Paturzo 
 TJUH 
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RE: [Histonet] Orange peel for GMS

[Bitting, Angela K.]

We just tried a moldy  strawberry. It was loaded with fungus!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Friday, April 17, 2015 7:11 AM
To: 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

I got a nice GMS control from some moldy chicken in my fridge. I am also trying 
the onion. I haven't stained the onion yet but it actually cut beautifully so I 
have high hopes. I let you know how it turns out. Have a nice weekend!

Jessica Piche, HT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of T Williams
Sent: Thursday, April 16, 2015 9:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Orange peel for GMS

I tried using a moldy orange peel as a GMS control, but the staining did not 
work.  I ran it in a 10 hour processing protocol.  Should I try something 
different?  Any suggestions would be extremely helpful - we're desperate for a 
GMS control.

Thanks,
T. Williams, HTL (ASCP), QIHC
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notify the sender immediately and delete these documents. Copyright (c) 
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[Histonet] lab dishwasher

[Bitting, Angela K.]

Can anyone recommend a good dishwasher for cleaning my special stains glassware?



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[Histonet] DPC4/SMAD4 on Ventana platform

[Bitting, Angela K.]

Has anyone had any luck optimizing DPC4 aka SMAD4 with Ventana's XT or 
BenchmarkULtra platform?
Any help is appreciated.

Angie



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[Histonet] Oil Red O

[Bitting, Angela K.]

Hello Fellow Histonetters,

We often get slides from another hospital for Oil Red O staining. On occasion, 
they send them at room temp. and until they get to us it's been a day or more.
I'm worried that because they are unfixed the cells will be autolyzed and I 
don't want to charge the patient for a useless stain.
  I haven't been able to find any guidance on how long a frozen section can sit 
at room temp. and still be acceptable to stain for Oil Red O.
Your thought and opinions please?

Angie




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RE: [Histonet] CBG Recycler users

[Bitting, Angela K.]

Thank you everyone for your replies and advice regarding my recycler.
As always, the best information comes from my esteemed peers on Histonet :)
Have a great day !!

-Original Message-
From: histot...@imagesbyhopper.com [mailto:histot...@imagesbyhopper.com] 
Sent: Tuesday, February 10, 2015 5:20 PM
To: Bitting, Angela K.
Subject: Re: [Histonet] CBG Recycler users

Hi Angie, I used it for quite a while, what's up?

Michelle

Sent from my iPhone

On Feb 10, 2015, at 10:40 AM, Bitting, Angela K. akbitt...@geisinger.edu 
wrote:



Would someone who is a CGB alcohol recycler user contact me? I can't get 
through to CBG.

Thanks,
Angie



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[Histonet] CBG Recycler users

[Bitting, Angela K.]

Would someone who is a CGB alcohol recycler user contact me? I can't get 
through to CBG.

Thanks,
Angie



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[Histonet] Ventana's MDM2 probe

[Bitting, Angela K.]

I'm looking for another institution who is running Ventana's MDM2 DNP probe. If 
you would be so kind as to contact me.
Thanks,
Angie



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RE: [Histonet] Benchmark Special Stainers

[Bitting, Angela K.]

Thank you everyone for your swift replies.

-Original Message-
From: STACEY [mailto:stace...@sbcglobal.net] 
Sent: Monday, February 02, 2015 3:57 PM
To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Benchmark Special Stainers

Yes, it is the same. (Carboy with a spigot.)

Stacey Merica, B.S., HT
Histology Supervisor
North Kansas City Hospital
North Kansas City, MO 64116

--
On Mon, 2/2/15, Bitting, Angela K. akbitt...@geisinger.edu wrote:

 Subject: [Histonet] Benchmark Special Stainers
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
 Date: Monday, February 2, 2015, 2:48 PM
 
 Can anyone tell me if the waste
 collection on the Benchmark Special Stainer is the same as  the waste 
collection on the Benchmark Ultra or XT? Carboy  with spigot??
 
 
 
 
 
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 Access to this message by anyone else is unauthorized. If  you are not the 
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 email. Thank you.
 
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[Histonet] Benchmark Special Stainers

[Bitting, Angela K.]

Can anyone tell me if the waste collection on the Benchmark Special Stainer is 
the same as the waste collection on the Benchmark Ultra or XT? Carboy with 
spigot??





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[Histonet] shelf life

[Bitting, Angela K.]

We are being inspected soon and I'm trying to check the labels on all of my 
chemicals.  How do I determine the expiration date of a 1N HCl solution I 
prepared in house.  I went on line and a commercially available 1N HCl solution 
has a 36 month shelf life according to the vendor's web site. I don't know how 
they arrived at that. I know concentrated HCl has an indefinite shelf life, but 
a 5 year retest date appears on the COA. It's confusing to me.
Any help would be appreciated.



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Re: [Histonet] TMA arrayer

[Bitting, Angela K.]

We also have the Grand Master. It's quite amazing, however we have had issues 
with the punches occasionally. Also alignment issues which turned out to be 
software related. Have you seen any of this Ashley??

Regards,
Angie

Sent from my iPhone

 On Dec 2, 2014, at 8:59 AM, Troutman, Kenneth A 
 ashley.trout...@vanderbilt.edu wrote:
 
 Hello Bernice,
 
 We have a TMA Grand Master from Perkin Elmer.  It is quite a machine.  Let me 
 know by email if you would like contact information.
 
 Regards,
 
 Ashley Troutman BS, MBA, HT(ASCP)QIHC
 Supervisor-Translational Pathology Shared Resource
 Vanderbilt University Medical Center
 S-1310 Medical Center North
 1161 21st Avenue South
 Nashville, TN  37232
 
 Message: 1
 Date: Mon, 1 Dec 2014 14:34:23 +
 From: Bernice Frederick 
 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu
 Subject: [Histonet] TMA arrayer
 To: Histonet 
 (histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu)

 histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
 Message-ID:

 62c639732d3f274daced033ebdf6adaf2f131...@evcspmbx2.ads.northwestern.edumailto:62c639732d3f274daced033ebdf6adaf2f131...@evcspmbx2.ads.northwestern.edu
 
 Content-Type: text/plain; charset=us-ascii
 
 Hello all,
 If anyone has a semi-automated arrayer, where did you get it? We have one 
 from Veridiam, but are wondering if anyone else makes them. We are looking to 
 purchase another unit.
 Bernice
 
 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edumailto:b-freder...@northwestern.edumailto:b-freder...@northwestern.edu%3cmailto:b-freder...@northwestern.edu
 
 
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[Histonet] RE: QIHC

[Bitting, Angela K.]

Yes, it's part of the requirements to be able to take the exam.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson
Sent: Wednesday, October 29, 2014 12:19 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FW: QIHC



From: Amy Johnson
Sent: Wednesday, October 29, 2014 6:57 AM
To: 'histonet-boun...@lists.utsouthwestern.edu'
Subject: QIHC

Do you need to have experience with ISH to take the QIHC test?

Thanks,
Amylin Johnson, BS HTL(ASCP)
Associates in Pathology
Wausau, WI
715-847-2130

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RE: [Histonet] Solvent Recyclers

[Bitting, Angela K.]

ditto

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Thursday, October 30, 2014 3:45 PM
To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Solvent Recyclers

CBG 
 http://www.cbgtechnologies.com/

Completely dependable.  I've had mine for years with no problems at all.  It 
runs forever!
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann 
Deathridge
Sent: Thursday, October 30, 2014 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Solvent Recyclers

Any recommendations for solvent recyclers?  Due to space issues benchtop unit 
preferred.  Dependability more preferred.
 Pros and cons?  You may email if preferred.  Thanks in advance for responses
  
 Maryann .
  
  madeathri...@pastnashville.com  

  




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[Histonet] Gram staining

[Bitting, Angela K.]

Our new Pathologist would like to see more yellow in the background of our Gram 
stain.  We use picric acid-acetone currently, which looks more pink than yellow 
most times. So  I'm wondering if a method using a Tartrazine counterstain would 
be better.
Any thoughts?


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[Histonet] microtome laceration not an exposure

[Bitting, Angela K.]

Does anyone have something that I can show to nursing to explain why a 
microtome laceration is not an exposure? I can't get the fixed tissue 
explanation to sink in. Anyone else had issues with this?




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[Histonet] PAS kit

[Bitting, Angela K.]

Is anyone else in Histoland having issues with the Ventana PAS kits? 
Particularly, crystals laying on top of the tissue after staining. If so, 
please contact me off line.

Thanks,
Angie

Angela K. Bitting, HT(ASCP),QIHC
Technical Specialist for Special Stains/ IHC/ISH
Geisinger Health System
100 N. Academy Avenue
Danville, PA 17822-2300
t. 570-214-9634
f. 570-271-5916



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RE: [Histonet] On the lighter side...

[Bitting, Angela K.]

28 years here

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeff L
Sent: Thursday, August 07, 2014 3:54 PM
To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

36 years here


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Thursday, August 07, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

36 years.

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Sullivan, Beatrice bsulli...@virtua.org
To: 'Douglas Porter' doug.por...@caplab.org; 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Thursday, August 7, 2014 1:51 PM
Subject: RE: [Histonet] On the lighter side...
 

44 years here.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???



Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street                                    
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

mailto:doug.por...@caplab.org doug.por...@caplab.org 

http://www.caplab.org/ www.caplab.org                              



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[Histonet] Desmoglein-3

[Bitting, Angela K.]

Ventana Benchmark Ultra users: Where are you buying your Desmoglein-3? I need 
an IVD product.

Thanks in advance,
Angie






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[Histonet] RE: multitissue control block

[Bitting, Angela K.]

We used to use a pc. Of proliferative endometrium, a pc of secretory 
emdometrium and a pc of cervix in a multitissue block for our ER/PgR control. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Friday, July 25, 2014 2:37 PM
To: Ortiz, Debra; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: multitissue control block

What is it that you want to know?  I used to make frozen multiple tissue 
control blocks for ER/PR many years ago before these antibodies could be used 
for FFPE tissues.  You could do the same thing with paraffin embedded tissue.  
I've made multi-tissue blocks using a skin biopsy punch tool to obtain small 
amounts of paraffin-embedded tissues to be used in the control block.  

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu on behalf of Ortiz, Debra 
debra.or...@advocatehealth.com
Sent: Friday, July 25, 2014 3:56 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] multitissue control block

Hello,

Could someone provide some information on the multi-tissue control block  used 
for IHC? Does anyone have one that is also used for ER/PR too.

Thank you

Debra Ortiz
Technical Director, AP
ACL Laboratories, Il
debra.or...@advocatehealth.com
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[Histonet] LOGOS One

[Bitting, Angela K.]

If anyone is currently using this processor I'd love to hear some feedback on 
this specific model.

Thanks,
Angie




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RE: [Histonet] Paraffin blocks

[Bitting, Angela K.]

Our facility handles paraffin shavings and blocks as Biological Waste which is 
different from Biohazardous waste.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, July 10, 2014 4:56 PM
To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet
Subject: RE: [Histonet] Paraffin blocks

I have been following this stream of conversation and thought I might jump in. 
Paraffin blocks and microtomy waste are NOT biohazard waste. The process of 
fixation and tissue processing makes the tissue acceptable for general waste 
disposal. You do have to address the issue of PHI on the blocks, if you are 
using bar  code information and especially if patient information is included 
in the bar code. All bar codes used in the lab can be read in any standard word 
or spreadsheet software. I do not see the need to pay for expensive removal of 
biohazard waste, use general waste disposal for your blocks and cutting waste.

William DeSalvo, BS HTL(ASCP)
 
 From: vriv...@westderm.com
 To: bcoo...@chla.usc.edu; jfra...@hotmail.com; 
 histonet@lists.utsouthwestern.edu
 Date: Thu, 10 Jul 2014 20:37:39 +
 Subject: RE: [Histonet] Paraffin blocks
 CC: 
 
 You would think, especially when you see the amount of waste a tech can 
 produce on high volume days, but we also discard ours into regular trash 
 too. Just curious how other facilities handle this type of waste.
 
 Regards,
 
 Vincent Rivera, HT (ASCP), QIHC, QLS
 Histopathology Supervisor
 West Dermatology Pathology Laboratory
 vriv...@westderm.com
 714-924-7240 (Lab)
 714-390-0906 (Cell)
 
 
 -Original Message-
 From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] 
 Sent: Thursday, July 10, 2014 1:30 PM
 To: Vincent Rivera; JOSEPH FRAZEE; histonet server
 Subject: RE: [Histonet] Paraffin blocks
 
 Good question.  So the linear path would dictate that they too should be 
 discarded into red bags, right?   You could also pose the same question about 
 the kimwipes used to skim your flotation baths.  We've always just thrown 
 both the shavings and kimwipes into the regular trash though.Guess we're 
 not so linear . . . 
 
 Thanks, 
 
 Brian
 
 
 -Original Message-
 From: Vincent Rivera [mailto:vriv...@westderm.com]
 Sent: Thursday, July 10, 2014 1:12 PM
 To: Cooper, Brian; JOSEPH FRAZEE; histonet server
 Subject: RE: [Histonet] Paraffin blocks
 
 I know this is slightly off topic but still along the same lines. How is 
 everybody handling their paraffin shaving waste? The waste that is produced 
 when trimming/facing tissue blocks? Is this also considered regulated medical 
 waste?
 
 Thanks,
 
 Vincent Rivera, HT (ASCP), QIHC, QLS
 Histopathology Supervisor
 West Dermatology Pathology Laboratory
 vriv...@westderm.com
 714-924-7240 (Lab)
 714-390-0906 (Cell)
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
 Sent: Thursday, July 10, 2014 12:23 PM
 To: JOSEPH FRAZEE; histonet server
 Subject: RE: [Histonet] Paraffin blocks
 
 In every institution I've ever worked, paraffin tissue blocks were handled as 
 regulated medical waste, and therefore discarded into red biohazard bags.
 
 Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology 
 and Laboratory Medicine Children's Hospital Los Angeles bcoo...@chla.usc.edu 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE
 Sent: Thursday, July 10, 2014 12:13 PM
 To: histonet server
 Subject: [Histonet] Paraffin blocks
 
 How is everyone disposing of paraffin blocks regular trash or biohazard 
 containers
 
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
 
 
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[Histonet] RE: Large slide rack and adaptor for Sakura stainer

[Bitting, Angela K.]

I posted about this earlier this year. Apparently they are no longer available 
in the US. I have found no substitute for these racks or adapter and Sakura was 
unwilling to help in any way. (Which I am still miffed about). 
If someone has a metal shop, here is a great opportunity.


Angela K. Bitting, HT (ASCP), QIHC
Technical Specialist IHC/Special Staining
Geisinger Medical Center
100 N. Academy Ave.
Danville, PA 17822-2300
Tel.570-214-9634





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, May 14, 2014 2:01 PM
To: Histonet
Subject: [Histonet] Large slide rack and adaptor for Sakura stainer

Hi all, Sakura has discontinued its large slide (50 x 75 mm) rack and metal 
adaptor for their Prisma stainer. Does anyone know a source for these that may 
have some left? Or do you have some you don't use anymore? The slide rack is a 
modified 24-slide Sakura rack that has cross bars on the bottom. The metal 
adaptors are inserts that slide in vertically on each end of the rack to space 
the slides.

The catalog numbers from Sakura are:

Large Slide Basket Sakura #4366
Adaptor for Large slides  #4365


Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


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RE: [Histonet] Ventana Benchmark Ultra and FITC antibody stains

[Bitting, Angela K.]

FITCs have to be purchased directly from Ventana. I've been trying to figure 
out how to trick their system because I have third partys that I'd like to 
try out. They don't offer an option to choose  a Prep kit in their FITC 
template. When I called in to ask why they don't allow it they told me they 
didn't have that much interest from people who wanted to do that.

Good luck,
Angie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman
Sent: Thursday, May 01, 2014 2:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana Benchmark Ultra and FITC antibody stains

Is anyone running FITC stains on the Ventana Benchmark Ultra?  Are you able to 
utilize Prep Kits or do the antibodies have to purchased directly from Ventana. 
 I appreciate your feedback.

 

Many Thanks,

Susan

 

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[Histonet] RE: Slide Consults

[Bitting, Angela K.]

I think it's pretty standard that outside institutions put their accession ID 
on the front of the consult slides. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, March 19, 2014 5:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide Consults


Would it be rude to ask other hospitals that review my slides to NOT WRITE 
their accession number on the front of my slides, but instead put their 
accession number on the back of my slides? Sometimes, I get slides back that 
have stickers on the front under my accession number or they'll hand write 
their accession number under mine.  I feel like I should scribble out the other 
institution number so that it doesn't confuse any of us refilling slides. What 
are your thoughts?  Would anyone like to share their slide send out form?

Thanks!

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[Histonet] RE: Activated carbon

[Bitting, Angela K.]

www.drsfostersmith.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Friday, January 24, 2014 10:51 AM
To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Activated carbon

I am looking for a low cost source for activated carbon. So far I am looking at 
$50 per pound for 6-14 mesh. This is for a VIP 300. Anybody use a different 
mesh size?



Thanks!

Mike
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RE: [Histonet] IHC-start-up

[Bitting, Angela K.]

So Mike,
It appears you are a sales director for the company, which makes your opinion 
null and void. Sorry.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Thompson
Sent: Thursday, January 23, 2014 1:10 PM
To: Colleen Forster
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC-start-up

Diagnostic Biosystems has the same platform with tech support that is 
outstanding!

Michael O. Thompson
Director of Sales
Diagnostic BioSystems
Phone: 1-888-896-3350
Mobile: 412-860-1288
Office Fax: 412-727-6080

IHC Made Affordable
 www.dbiosys.com

Colleen Forster cfors...@umn.edu wrote:

I totally agree with William. I do animal IHC and I am, using the 
Biocare Nemesis, which be the same as the Dako platform. Completely 
open so you can do what you need to at a cost you can afford

Colleen Forster HT(ASCP)QIHC
u of MN



On 1/23/2014 11:44 AM, Will Chappell wrote:
 Doing ihc on animal tissue is very tricky, if for no other reason than you 
 are trying to standardize an antibody across multiple species. You will need 
 to use every trick in the book and invent some of your own to get 
 reproducible results.

 I would steer clear of any platforms that do not let you customize 
 everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have 
 their quirks but they have the openness you require.

 William Chappell

 Sent from my iPhone

 On Jan 23, 2014, at 11:49 AM, Erin Sarricks esarri...@gmail.com wrote:

 Hi all-

 Our histopathology lab is looking to set up an IHC component to 
 service the request of our clients.  All work is on animal tissue.  We will 
 probably run about 1,000 IHC slides the first year and hope to increase the 
 workload each year.  Some  stains we would run would include CD4, CD8, 
 Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a 
 good fit for our needs.  Does anyone have any other advice on other 
 machines we should look into?  Any information or advice would be greatly 
 appreciated. Thank you!

 Regards,

 Erin Sarricks, HT (ASCP)
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[Histonet] RE: Thermo Scientific Embedding Centre Histostar

[Bitting, Angela K.]

We had one screen replaced early on but no problems since. However, we've had 
to replace the plastic lids several times and the parts are always on back 
order. The one that covers the molds breaks right off.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne
Sent: Tuesday, January 21, 2014 2:54 PM
To: 'Gregoire, Rhonda (MAFRI)'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Thermo Scientific Embedding Centre Histostar

I have the Histostar embedding center (purchased 9/2012) and have not had any 
problems with the touch screen.  

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT  05641
802-371-4923
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gregoire, 
Rhonda (MAFRI)
Sent: Tuesday, January 21, 2014 1:09 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Thermo Scientific Embedding Centre Histostar

We are looking at purchasing a Histostar and would like to know if you have had 
any trouble with the touch screen?  I had to replace the touch screen on our 
Histocenter 3 because we could no longer navigate the menu with all the lines 
going thru it.  Wanted to know if it was just our bad luck or if anyone else 
has had the same experience with the touch screen.

Thanks

Rhonda Gregoire, MLT
Supervisor, Clinical Pathology
Veterinary Diagnostic Services
Manitoba Agriculture, Food and Rural Development
545 University Crescent
Winnipeg, MB
R3T 5S6

phone 204-945-7641
fax 204-945-7646
email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca



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RE: [Histonet] Recuts/deepers

[Bitting, Angela K.]

We track shallow/incomplete sections and have found numerous reasons. 
Inadequate fixation. 
The way a tissue is bisected can cause it to not lay flat in the embedding 
mold. For anstance a grossing tech who does a wrist turn kind of slicing 
motion and causes an arch shaped edge. 
Embedding pieces that are varying sizes. 
Too many pieces in a cassette. 
Some Histotechs are trained to err on the side of caution and do not like to 
cut too deep the first time. Their though being you can always come back  and 
recut it, but once it's cut away, it's gone. 

Our idea was that by tracking these we might find an opportunity for retraining 
an individual, but as it turns out, we all cut a shallow/incomplete section 
occasionally. 
Some steps we took -
  We had the grossing techs come and watch us embed derm cases. That gave them 
a perspective of why it's important for them to do things a certain way. 
 One of our Dermatopathologists came in and gave the HTs and in-service on what 
they are looking to see on a slide and what the inked margins mean. This helped 
with orienting specimens at embedding. 
 We have reduced our recut numbers by taking the time to identify the causes 
and offering additional training to the staff. Collecting the data is the first 
step, and though it's time-consuming, it is worth it in the end.
Best wishes.
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, January 13, 2014 11:36 PM
To: Trini; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Recuts/deepers

What are the reasons they ask for recuts? The answer to that will give you some 
ideas. For instance, did they not get a full face with margins the first time? 
Was the section not readable? Was the stain inadequate?

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Trini
Sent: Monday, January 13, 2014 4:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recuts/deepers

Hello histonetters! 

Could I please get some help/advice/ideas on how to reduce recut/deeper 
requests? What does your lab do to reduce that? Would you say embedding is 
partially the problem? Such as small tissue embedded with bigger tissue? Etc? 


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RE: [Histonet] Safety items

[Bitting, Angela K.]

That should be the slogan for NSH next year :D

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, January 07, 2014 11:26 AM
To: Rathborne, Toni; 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

If it isn't documented it isn't done!  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Tuesday, January 07, 2014 11:09 AM
To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Are they looking for documentation that it's been checked, or will they take 
your word for it?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert 
A
Sent: Tuesday, January 07, 2014 10:51 AM
To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Newest CAP inspection stupid thing. They want you to periodically check your 
spill kit for clumping, since it does not have an expiration date. I challenged 
it, but don't know if will go through. 

Robert A. Eytalis
Laboratory Manager
robert-eyta...@riversidehealthcare.net
Phone: (815) 935-7256 ext. 5186
(815) 935-7535
Fax (815) 935-7068

Riverside Medical Center
350 N. Wall Street - Kankakee, IL 60901


http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.riversidemc.net%2f
  |  
http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh 
[amurv...@advancederm.net]
Sent: Tuesday, January 07, 2014 9:25 AM
To: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Don't forget to make sure you have a spill kit and its labeled well with easy 
access,  Inspectors will ask employees if they know where it and your fire 
extinguisher and alarm are located. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, January 07, 2014 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safety items


What safety items do you keep in your lab besides the normal apron, gloves, eye 
wash stations and shower?  Thanks!

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RE: [Histonet] NCCI interpretation for ISH coding

[Bitting, Angela K.]

That's my understanding as well.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Monday, January 06, 2014 6:03 PM
To: Johns, Jill
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] NCCI interpretation for ISH coding

My understanding was that this is just for Medicare patients...



On Mon, Jan 6, 2014 at 12:35 PM, Johns, Jill jjoh...@cpallab.com wrote:

 The following was taken from the NCCI manual, Chapter 10, effective 1/1/14:

 9. The unit of service for in situ hybridization reported as CPT codes 
 88365, 88367, or 88368 is each probe staining procedure per specimen. 
 If a single probe staining procedure for one or more probes is 
 performed on multiple blocks from a surgical specimen, multiple slides 
 from a cytologic specimen, or multiple slides from a hematologic 
 specimen, only one unit of service may be reported for each separate 
 specimen. Physicians should not report more than one unit of service 
 for CPT codes 88365, 88367, or 88368 per specimen for a probe staining 
 procedure even if it contains multiple separately interpretable probes.

 I'm wondering how other labs are interpreting this, because I'm not 
 sure how we continue to offer FISH testing and not go in the hole 
 financially, if we can only bill for ONE probe on ONE specimen, 
 regardless of the actual number of interpretable probes (Example: 
 currently, for a HER2 FISH test, we bill for 2 units of service--1 for 
 the HER2 probe and 1 for the CEP17 probe--both of which have to be 
 enumerated by a professional and a ratio generated). Any insight from others 
 would be greatly appreciatedThanks!


 Jill A. Johns, MT(ASCP)SH, QCym, CCy
 Manager of Molecular Pathology
 Central PA Alliance Laboratory (CPAL)
 1803 Mt. Rose Ave., Suite C3/C4
 York,  PA17403
 phone: (717) 851-4320
 fax:  (717) 851-1450
 email:  jjoh...@cpallab.commailto:jjoh...@cpallab.com


 
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[Histonet] RE: Yahoo link

[Bitting, Angela K.]

The article doesn't even mention the repetitive motion injuries.




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RE: [Histonet] This is not Facebook

[Bitting, Angela K.]

Yeah, wow, I just read through this thread I guess haters are gonna hate. 
Nobody's got time for that.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Wednesday, September 04, 2013 12:55 PM
To: 'Marsh, Nannette'; ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] This is not Facebook

I responded to jw privately but want to show my support for Hazel or anyone 
else that is facing a CAP inspection.  I think Hazel and everyone that posts 
that CAP is in their institution is looking for moral support and I sent Hazel 
a message right away saying Good luck.  I hope Hazel or anyone else posts how 
their inspection goes and lets the rest of us know any issues we should be on 
the look out for!~

We all know how trying it can be to be inspected  Just hit delete if you 
don't want to be friedly and show your support!!!


Dawn D. Schneider, HT(ASCP)
Lead HT
Howard Young Medical Center
240 Maple Ave.
Woodruff, WI 54558
715-356-8174

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marsh, Nannette
Sent: Wednesday, September 04, 2013 8:48 AM
To: ihcs-wojcieszyn; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] This is not Facebook

Wow, life is too short.  We don't have to take things so seriously all of the 
time.  I do hope your day gets better :-)

Nanne Marsh HT ASCP

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ihcs-wojcieszyn
Sent: Tuesday, September 03, 2013 8:36 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] This is not Facebook

E-mails that tell all of what is happening, i.e. CAP is in house is a waste 
of time. Are we going to communicate when you wash your hands or have a cup of 
coffee.
If Histonet is to be a source of information, ASK a question


jw


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[Histonet] RE: Labels for outside blocks

[Bitting, Angela K.]

If you keep the layer of paraffin thin enough, it can still be scanned with 
most barcode scanners.

-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com] 
Sent: Thursday, August 29, 2013 11:15 AM
To: Bitting, Angela K.; Morken, Timothy; Histonet
Subject: RE: Labels for outside blocks

I do the same thing.  Adding a couple of drops of paraffin over the label has 
worked for us for years.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Thursday, August 29, 2013 10:43 AM
To: Morken, Timothy; Histonet
Subject: [Histonet] RE: Labels for outside blocks

We get the label with the block and stick it on ourselves with melted paraffin.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, August 28, 2013 4:41 PM
To: Histonet
Subject: [Histonet] Labels for outside blocks

Has anyone found a very sticky label for labeling outside consult blocks,  ie 
that won't fall off?

Tim Morken
UCSF Pathology

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RE: [Histonet] Borrelia burgdorferi IHC

[Bitting, Angela K.]

I am using a polyclonal antibody to B. burgdorferi. Item #BP1002 from Acris 
Antibodies.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Wednesday, August 14, 2013 1:39 PM
To: Delray Beach Pathology Kari Simeone
Cc: histonet
Subject: Re: [Histonet] Borrelia burgdorferi IHC

Meridian Life Science has monoclonal and polyclonal antibodies to B.
burgdorferi.  None are ready-to-use, but two are conjugated (either to HRP or 
Biotin).

Jan Shivers
UMN


On Wed, Aug 14, 2013 at 12:12 PM, Delray Beach Pathology Kari Simeone  
ksime...@leavittmgt.com wrote:

 Can anyone recommend a vendor where I can purchase ready to use 
 Borrelia burgdorferi antibody for IHC? Thanks in advance.

 Kari M Simeone
 Histology/Immunohistochemistry Specialist Supervisor Alternate 
 Laboratory Supervisor ADCS Clinics, LLC Advanced Dermatology and 
 Cosmetic Surgery Ameriderm
 561.819.0857 ext 100
 561.819.6517 fax
 ksime...@leavittmgt.com
 www.advancedderm.com http://www.advancedderm.com/

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[Histonet] RE: Histology Video

[Bitting, Angela K.]

I saw a few a while back. Pretty funny. It looks like they were made by some 
Pathology residents and techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, July 18, 2013 1:02 PM
To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histology Video

Let's make one!

Actually, I probably am not allowed to...but the rest of you could!  Go for it!

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Thursday, July 18, 2013 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Video

Does anyone know of any Histology parody videos out there on YouTube?  Just 
curious!

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

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RE: [Histonet] HPV ISH Probes

[Bitting, Angela K.]

I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit 
Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, 
Montgomery, TX 77356, 1-800-338-9579

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia
Sent: Tuesday, June 25, 2013 4:33 PM
To: Houston, Ronald; joelle weaver; Susan Foreman; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HPV ISH Probes

I have personally tried the Dako probes on the Ventana platform but no luck so 
far. 

I have heard about the Enzo probes been succesfully worked up at other 
institutions. Does anybody know if a 'link antibody' is needed for the Enzo 
probes? 

Greatly appreciate any inputs. 

Cecilia M. Obregon, HTL (ASCP)
Memorial Regional Hospital
3501 Johnson Street
Hollywood, FL 33021
Ph: 954-265-5317

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Houston, Ronald 
[ronald.hous...@nationwidechildrens.org]
Sent: Tuesday, June 25, 2013 3:41 PM
To: joelle weaver; Susan Foreman; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HPV ISH Probes

Many people are working on the Enzo probes just now. Mayo does have the probes 
up and running on the Bonds, but are keeping tight-lipped about their protocols.
We tried the Dako probes but never could get consistent results.

Ronnie Houston MS HT(ASCP)QIHC

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, June 25, 2013 3:34 PM
To: Susan Foreman; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HPV ISH Probes

HPV6, 11, 16 18, 31, 33  51 screening'




Joelle Weaver MAOM, HTL (ASCP) QIHC

 From: sfore...@labpath.com
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 25 Jun 2013 14:10:59 -0400
 Subject: [Histonet] HPV ISH Probes

 Does anyone have HPV-ISH working?  utilizing a vendor for the probe 
 other than Ventana?  Prep Kit?





 Many Thanks,

 Susan Foreman, HT (ASCP)
 (865)584-1933





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[Histonet] RE: Control Storage

[Bitting, Angela K.]

Something to remember is that humidity is the enemy too. If you are storing 
your slides in a refrigerator make sure that the humidity is well controlled. 
We found out the hard way.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Wednesday, June 12, 2013 11:56 AM
To: Herring, Colleen; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Control Storage

We store our blocks at room temp, our IHC slides are stored in the fridge.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen 
[colleen_herr...@bshsi.org]
Sent: Wednesday, June 12, 2013 9:48 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Storage

Does anyone have any ideas or suggestions about the storage of blocks and 
precut slides for immunos?


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RE: [Histonet] Is the server down?

[Bitting, Angela K.]

Vikki, Didn't they tell you?? You were voted off the island.
:)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Tuesday, April 23, 2013 2:10 PM
To: Histo Net list server
Subject: [Histonet] Is the server down?

Haven't seen any posts over past day or so.
Vikki
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RE: [Histonet] Batch Controlling for IHC

[Bitting, Angela K.]

Technically, he would be right. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Tuesday, March 26, 2013 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Batch Controlling for IHC


Hello All,
 
Have you ever had a Pathologist say, if you have one tissue control (placed on 
the same slide as the patient tissue) per antibody, per patient case, but there 
are multiple blocks (same tissue type) from the same patient case, that would 
be batch controlling?  
 
Hope that made sense, just wondering if this is something others have dealt 
with.
 
Thanks,
 
Tim
 
  
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RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)

[Bitting, Angela K.]

We hate Leica's CV5030 coverslipper too.!!! Babysit is a good word to 
describe the user experience.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, March 20, 2013 7:32 PM
To: Contact HistoCare; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)

I agree. The Leica stainer and Sakura are both good instruments, however I 
really HATE that Leica glass cover slipper and I had the same assessment as to 
need to baby sit. I sometimes preferred just hand cover slipping because it 
was less trouble, and even faster sometimes ( believe that or not). I had none 
of these issues with the Prisma covcr slipper, and no trouble with the user 
interface etc. I had the same issue with the door latch on the Prisma, but 
other than a quick replacement of that, it worked perfectly.




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: cont...@histocare.com
 Date: Wed, 20 Mar 2013 17:49:22 -0500
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)
 
 Both stainers are powerhouses. The Leica has a plain menu screen with a 
 simple interface while the Sakura has a LCD screen with detailed information 
 about what stage the staining process a rack is along with multiple menus.  
 The difference between the performance changes drastically when the 
 respective coverslipper attachments become involved.
 
 The Leica is seriously no match for the Sakura in this respect. The Leica's 
 coverslipper is its Achilles heel and requires a LOT more attention and 
 alerts frequently, very frequently. It takes a separate rack for staining the 
 slides at the beginning of the process and eventually transfers them to a 
 different rack one the cover slip is complete. This one uses glass and 
 frequently drops glass, creates bubbles, drops and breaks slides. You will 
 have to frequently purge the system and clean the cover medium needle 
 dropper.  Once done, it only holds. Two racks of 30 slides and will alert 
 until you remove it. You can't leave this one alone for more than 5 minutes 
 without an alert. Seriously.
 
 The Sakura's coverslipper uses cover tape which won't need to be replaced not 
 even remotely as soon as the glass in the Leica.  Finished slides remain in a 
 carousel at the top and can hold about 10 racks of 20 before it alerts. For 
 high volume, the Sakura pair wins hands down. You won't lose productivity 
 time by needing to check on this machine pair.
 
 HistoCare.com
 
 
 
 
 
 
 Hi,
 
 We are currently looking to switch out our linear MKII stainer for 
 either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any 
 recommendations?
 Are quantity of HEs is increasing and we need adequate equipment to 
 meet our workload. The incorporated oven seems excellent on both 
 stainers. Any pros/cons would be greatly appreciated! Also, if you are 
 currently using the stainer, does it meet your workload and what is your 
 volume?
 
 Thanks!
 
 Sophia
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RE: [Histonet] Billing for Pin 4 Cocktail

[Bitting, Angela K.]

I was given info from a Dako rep last week that if the primary antibodies are 
applied separately to the slide as is true with Ventana instruments, you can 
charge for  both.  I am skeptical. Has anyone else heard this?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez
Sent: Friday, March 15, 2013 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Billing for Pin 4 Cocktail


Its actually not allowed already.  Any cocktail where all stains are done at 
one time on one slide can only be charged x1 even if its 2 or more antibodys in 
the cocktail.  One of our reps updated us with our PIN cocktail



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Friday, March 15, 2013 11:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Billing for Pin 4 Cocktail

Our lab currently bills for three, and I know of other labs in our area that 
also bill for three. I have heard rumors that this may not be allowed in the 
near future.
 
Roger Heyna
Maywood, IL

 Debbie Granato debgran...@yahoo.com 3/15/2013 11:00 AM 
We have a billing question for the PIN4 Cocktail that we perform on prostate 
needle biopsies.
We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in 
the lab.
How would you bill for this?
Would billing for 1 stain be correct or can you bill for 3 stains?

Thank you for your input.
Debbie Granato

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RE: [Histonet] Ventana HPV High/Low Replacements?

[Bitting, Angela K.]

Just spoke with my Ventana rep about this same topic. My understanding is ANY 
vendor who sells HPV probe for ISH is out of compliance with FDA and is 
eventually going to get in trouble. I specifically asked if I could buy probe 
from elsewhere and was told not likely. This is only a problem for us here in 
the US because of FDAs restrictions. I hope someone will tell me I 
misunderstood. Ventana suggests running p16 if you don't already. Just throwing 
in my 2 cents.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
Masuzumi
Sent: Friday, March 01, 2013 2:09 PM
To: Histonet
Subject: [Histonet] Ventana HPV High/Low Replacements?

Hello everyone,

My lab is interested in starting HPV High and Low Risk ISH testing, but it 
seems like we just missed the boat on it. We have Benchmark XTs, but Ventana 
discontinued their HPV probes sometime around last December, and they don't 
have an alternative at this time. After talking with nearby labs that are 
currently using stockpiles of Ventana probes, they told me the following 
courses of action they were planning on using when their stock runs out:
-One lab is thinking of doing HPV by PCR -Another is thinking of switching to 
Dako probes

After discussing the possibility of Dako probes with Ventana and Dako, neither 
could recommend using them together because each side was unfamiliar with the 
other's reagents or system, though both said it might be possible.

Is anybody in a similar situation, looking for a replacement for the Ventana 
HPV High/Low Risk probes? Any insight on possibilities to pursue would be much 
appreciated!

Sincerely,
 
Jennifer Masuzumi
Sterling Pathology
Seal Beach, CA
jennifer.masuz...@sterlingpath.com
562-799-8900
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RE: [Histonet] Ventana Labels

[Bitting, Angela K.]

I just tried them Friday. They worked fine but the label picked up counterstain 
a bit. However, depending on the cost per label, it may be worth the trouble. 
I'm waiting for a quote.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Roark
Sent: Monday, February 25, 2013 2:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana Labels

Hello all,

I am getting ready to try some slide labels as an alternative to the Ventana 
labels.  I was wondering if anyone else is using them or have tried them in the 
past.

They do not have that plastic fold over flap and are suppose to be quite 
economical, though I have not got a quote yet. 

They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - 
Mercedes Medical Supply sent me the trial roll.

Thanks!


Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
mro...@sfmc.net
http://www.sfmc.net





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[Histonet] RE: Antibody IDH1

[Bitting, Angela K.]

Are you using the antibody from Dianova??

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, 
Billie
Sent: Friday, February 22, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody IDH1

Does anyone perform this antibody?? We can't get it to work on the Ventana 
Ultra.  PhenoPath in Seattle performs it on the Dako autostainer using a small 
size polymer detection kit.  I would appreciate any input.

Thanks,

Billie Zimmerman


Augusta State University and Georgia Health Sciences University have 
consolidated to become Georgia Regents University. Effective January 9, 2013, 
my email address has changed to bzimm...@gru.edu. Please update your address 
book to reflect this change.
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RE: [Histonet] Antibody IDH1

[Bitting, Angela K.]

I received a spec sheet from Dianova that has their Ventana XT protocol. It 
suggests using CC2 standard retrieval and incubation for 32 minutes with amp. 
They give a dilution range of 1:20-1:50. It seems to work well for us although 
we are still validating. I hope that helps.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Friday, February 22, 2013 4:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibody IDH1

Billie,
 
We use Dianova's antibody and purchase it from Fisher. We've performed this 
stain for a little over six months and seem to be having success with the 
following protocol:
 
Dianova's IDH1 R132H Antibody, Diluted 1:100 Ventana Benchmark XT, iView 
Detection Standard CC1
37 degrees C
32 minute antibody incubation
A/B Block
 
I hope this helps!
 
Roger Heyna
Maywood, IL

 Zimmerman, Billie bzimm...@gru.edu 2/22/2013 3:20 PM 
Does anyone perform this antibody?? We can't get it to work on the Ventana 
Ultra.  PhenoPath in Seattle performs it on the Dako autostainer using a small 
size polymer detection kit.  I would appreciate any input.

Thanks,

Billie Zimmerman


Augusta State University and Georgia Health Sciences University have 
consolidated to become Georgia Regents University. Effective January 9, 2013, 
my email address has changed to bzimm...@gru.edu. Please update your address 
book to reflect this change.
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RE: [Histonet] Space requirements for AP lab

[Bitting, Angela K.]

Well, if it's more than 2.5 sq. ft per tech, we're out of compliance :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jmasla...@stpetes.org
Sent: Monday, December 10, 2012 12:41 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Space requirements for AP lab

Greetings All, Are there any JACHO or CAP space requirements for an AP 
laboratory? Looking for recommended square footage per tech, equipment, and/or 
work area.
Thanks


Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126 

Give thanks for ALL things.
Kindness is the language the blind can see  the deaf can hear- Mark Twain 
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RE: [Histonet] 88305TC starting to hit the fan...

[Bitting, Angela K.]

Histonet should stay free of those entanglements(political opinions)
Funny, when I wrote that here 2 weeks before the election no one wanted to hear 
it.
What has changed??


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stanicar, 
Jennifer
Sent: Tuesday, November 20, 2012 10:53 AM
To: 'ihcman2...@hotmail.com'; David Kemler; Fellow HistoNetters
Subject: RE: [Histonet] 88305TC starting to hit the fan...

Thank you for that Glen.  I agree!

Jen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
ihcman2...@hotmail.com
Sent: Tuesday, November 20, 2012 8:40 AM
To: David Kemler; Fellow HistoNetters
Subject: Re: [Histonet] 88305TC starting to hit the fan...

We can all see sour grapes about the election by simply turning on any news 
channel.  Histonet should stay free of those entanglements and remain focused 
on discussion and problem solving.

My opinion.
Glen Dawson
Janesville, WI 


Sent from my U.S. Cellular Android device

- Reply message -
From: David Kemler histot...@yahoo.com
Date: Mon, Nov 19, 2012 8:49 pm
Subject: [Histonet] 88305TC starting to hit the fan...
To: Fellow HistoNetters Histonet@Lists.UTSouthwestern.edu

Hmmm...I think more people should have paid attention to Obamacare two years 
ago when it was being shoved down eveyones throat. Oh well.. as old saying 
goes...You ain't seen nottin' yet! I'm just amazed that there are so many in 
the profession who are surprised about the changes beginning to take place, 
there are many, many more to come in 2013  14 and all of them affect your job 
or lack thereof. :)
 
Everyone had the opportunity to change things on November 6th - they chose not 
to. So, you live with it.
 
Yours,
David
 
 



From: Davide Costanzo pathloc...@gmail.com
To: Webster, Thomas S. twebs...@crh.org
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Monday, November 19, 2012 6:46 PM
Subject: Re: [Histonet] 88305TC starting to hit the fan...

While this stinks on many levels, I have to take issue with the shift wealth 
from specialists to family practice - family practice docs have been the 
frontline of medicine, all the while earning less than a quarter of what 
specialists earn. It's about time they get a boost.
Too many specialists earn over a million a year, while the family practice 
guys/ladies can barely pay their student loans.

Sent from my iPhone

On Nov 19, 2012, at 1:25 PM, Webster, Thomas S. twebs...@crh.org wrote:

 CAP had a webinar last week about the cut. These are some very scary times. 
 For some reason the government has decided to shift wealth from specialists 
 to family practice. I am becoming more angry with the affordable care act 
 everyday.

 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverrid
 e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtl
 t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinar
 s.html_state=maximized_pageLabel=cntvwr


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 appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 47201___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] 88305TC starting to hit the fan...

[Bitting, Angela K.]

Are we a bunch of catty 13 year olds or can we stick to Healthcare? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo
Sent: Tuesday, November 20, 2012 10:31 AM
To: Rene J Buesa
Cc: Fellow HistoNetters
Subject: Re: [Histonet] 88305TC starting to hit the fan...

Tell them Rene! Too many Fox News listeners here I suspect.

Sent from my iPhone

On Nov 20, 2012, at 6:05 AM, Rene J Buesa rjbu...@yahoo.com wrote:

 The law provides economic assistance to those with low income who cannot 
 afford insurance.
 It would be nice if we all read the law instead of paying attention to those 
 who try to scare people.
 René J.


 
 From: Bernice Frederick b-freder...@northwestern.edu
 To: Rene J Buesa rjbu...@yahoo.com; David Kemler 
 histot...@yahoo.com; Fellow HistoNetters 
 Histonet@Lists.UTSouthwestern.edu
 Sent: Tuesday, November 20, 2012 9:00 AM
 Subject: RE: [Histonet] 88305TC starting to hit the fan...

 But can they afford to buy it

 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 ECOGPCO-RL
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edu


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J 
 Buesa
 Sent: Tuesday, November 20, 2012 7:58 AM
 To: David Kemler; Fellow HistoNetters
 Subject: Re: [Histonet] 88305TC starting to hit the fan...

 Tell that to all those who have pre-existing medical conditions that can now 
 buy health insurance.
 René J.


 
 From: David Kemler histot...@yahoo.com
 To: Fellow HistoNetters Histonet@Lists.UTSouthwestern.edu
 Sent: Monday, November 19, 2012 9:49 PM
 Subject: Re: [Histonet] 88305TC starting to hit the fan...

 Hmmm...I think more people should have paid attention to Obamacare two 
 years ago when it was being shoved down eveyones throat. Oh well.. as 
 old saying goes...You ain't seen nottin' yet! I'm just amazed that 
 there are so many in the profession who are surprised about the 
 changes beginning to take place, there are many, many more to come in 
 2013  14 and all of them affect your job or lack thereof. :)

 Everyone had the opportunity to change things on November 6th - they chose 
 not to. So, you live with it.

 Yours,
 David




 
 From: Davide Costanzo pathloc...@gmail.com
 To: Webster, Thomas S. twebs...@crh.org
 Cc: histonet@lists.utsouthwestern.edu 
 histonet@lists.utsouthwestern.edu
 Sent: Monday, November 19, 2012 6:46 PM
 Subject: Re: [Histonet] 88305TC starting to hit the fan...

 While this stinks on many levels, I have to take issue with the shift wealth 
 from specialists to family practice - family practice docs have been the 
 frontline of medicine, all the while earning less than a quarter of what 
 specialists earn. It's about time they get a boost.
 Too many specialists earn over a million a year, while the family practice 
 guys/ladies can barely pay their student loans.

 Sent from my iPhone

 On Nov 19, 2012, at 1:25 PM, Webster, Thomas S. twebs...@crh.org wrote:

 CAP had a webinar last week about the cut. These are some very scary times. 
 For some reason the government has decided to shift wealth from specialists 
 to family practice. I am becoming more angry with the affordable care act 
 everyday.

 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverri
 d 
 e=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPt
 l 
 t%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webina
 r s.html_state=maximized_pageLabel=cntvwr


 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use 
 of the intended recipient(s) and may contain confidential and 
 privileged information. You may NOT use, disclose, copy or 
 disseminate this information.  If you are not the intended recipient, 
 please contact the sender by reply e-mail immediately.  Please 
 destroy all copies of the original message and all attachments. Your 
 cooperation is greatly appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 
 47201___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 

RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) andfuture trends

[Bitting, Angela K.]

Here we go with the political opinions again. They have no place on this forum. 
It's bad enough that Facebook is polluted with them.

Have a nice day!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 31, 2012 11:03 AM
To: Kaye Ryan
Cc: Histonet
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

Kaye:
Sure!
The odd thing that struck me is that this fictitious scenario was planted 
on Histonet just a few day before the presidential election and I am suspicious 
of the final intent of the posting.
Is it to link a frightening scenario with the so called Obama care?
Is it the possible solution of this imaginary scenario to eliminate Obama 
care as has been promised by Romney?
Is this a way to get a few additinal votes for Romney? I would not be surprised 
at all!
I am always very suspicious of coincidences because coincidences do not 
exist.
René J.



From: Kaye Ryan kr...@nfderm.com
To: Rene J Buesa rjbu...@yahoo.com
Sent: Wednesday, October 31, 2012 10:55 AM
Subject: RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

THANK YOU SO MUCH, RENEE!

Kaye

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 31, 2012 10:51 AM
To: Brendal Finlay; Webster, Thomas S.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

To everybody worrying for something that has NOT happen yet and that NOBODY 
knows if will happen.
These are pure speculations.
Do not put the cart before the horses. I do not think that this scary scenario 
is in the near future.
René J.



From: Brendal Finlay brendal.fin...@medicalcenterclinic.com
To: Webster, Thomas S. twebs...@crh.org
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wednesday, October 31, 2012 9:39 AM
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) and 
future trends

This is disturbing news. As an employee of an in-house lab (which started in 
1996/1997) that does mostly skins, GI biopsies, and outpatient surgery 
specimens I'm pretty disheartened to hear about the 88305 issue. Melanoma 
excisions, prostates (even lower block # cases, we don't always get 12), breast 
biopsies, and other more difficult cases can be a lot of work on both the 
professional  technical end of things.  

As for prostate biopsies, CMS has already lowered reimbursement with the G 
codes. This is despite the wording that they are for saturation biopsies. We 
rarely have saturation biopsies, but Medicare denies us the 88305 charge if 
more than 5 specimens. 

Other insurance companies tend to follow their lead  after a little time.  I 
believe reimbursement is 50-75% less for 5-20 biopsies, but don't quote me on 
that.  I expect we may see the end of saturation and multi-container prostate 
biopsies in the near future. 

Another issue for many outpatient labs in my area is that larger insurances are 
requiring their patients to go to large multinational labs.  We cannot accept 
many PPOs or Medicare replacement plans because of this. 

I feel it can be a disservice to the patient because they do not get the same 
personal, local service with good turn around times. Even my insurance requires 
me to go to one of these labs where I feel inconvenienced and frustrated at the 
wait time required to submit my sample and get results to my physician. 

On Oct 31, 2012, at 8:17 AM, Webster, Thomas S. twebs...@crh.org wrote:

 Here is what CAP has on their website about the issue.
 Only the TC of 88305 is being discussed for 2013. We should know fairly soon 
 the decision.
 More codes have been flagged as overvalued as well that could be cut for 2014 
 (PC and TC at this point).
 
 http://www.cap.org/apps/docs/advocacy/advocacy_issues/revaluation.pdf
 
 
 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use of 
 the intended recipient(s) and may contain confidential and privileged 
 information. You may NOT use, disclose, copy or disseminate this 
 information.  If you are not the intended recipient, please contact 
 the sender by reply e-mail immediately.  Please destroy all copies of 
 the original message and all attachments. Your cooperation is greatly 
 appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 47201___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu

RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) andfuture trends

[Bitting, Angela K.]

I'm going back to Facebook now. Let me know when this thread poops out.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hermina 
Borgerink
Sent: Wednesday, October 31, 2012 11:33 AM
To: Rene J Buesa; Kaye Ryan
Cc: Histonet
Subject: RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

Thank you!!! I couldn't agree with you more. Those were my exact thoughts when 
I read the initial e-mail.

Hermina 

Hermina M. Borgerink, MA, HT(ASCP)HTL, QIHC Wake Forest University Primate 
Center Department of Pathology Medical Center Blvd.
Winston-Salem, N C  27157
Tel. (336) 716-1538
Fax (336) 716-1515
Email: hborg...@wakehealth.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 31, 2012 11:03 AM
To: Kaye Ryan
Cc: Histonet
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

Kaye:
Sure!
The odd thing that struck me is that this fictitious scenario was planted 
on Histonet just a few day before the presidential election and I am suspicious 
of the final intent of the posting.
Is it to link a frightening scenario with the so called Obama care?
Is it the possible solution of this imaginary scenario to eliminate Obama 
care as has been promised by Romney?
Is this a way to get a few additinal votes for Romney? I would not be surprised 
at all!
I am always very suspicious of coincidences because coincidences do not 
exist.
René J.



From: Kaye Ryan kr...@nfderm.com
To: Rene J Buesa rjbu...@yahoo.com
Sent: Wednesday, October 31, 2012 10:55 AM
Subject: RE: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

THANK YOU SO MUCH, RENEE!

Kaye

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, October 31, 2012 10:51 AM
To: Brendal Finlay; Webster, Thomas S.
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) 
andfuture trends

To everybody worrying for something that has NOT happen yet and that NOBODY 
knows if will happen.
These are pure speculations.
Do not put the cart before the horses. I do not think that this scary scenario 
is in the near future.
René J.



From: Brendal Finlay brendal.fin...@medicalcenterclinic.com
To: Webster, Thomas S. twebs...@crh.org
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wednesday, October 31, 2012 9:39 AM
Subject: Re: [Histonet] The Rise of Physician Owned/Operated Labs (POLs) and 
future trends

This is disturbing news. As an employee of an in-house lab (which started in 
1996/1997) that does mostly skins, GI biopsies, and outpatient surgery 
specimens I'm pretty disheartened to hear about the 88305 issue. Melanoma 
excisions, prostates (even lower block # cases, we don't always get 12), breast 
biopsies, and other more difficult cases can be a lot of work on both the 
professional  technical end of things.  

As for prostate biopsies, CMS has already lowered reimbursement with the G 
codes. This is despite the wording that they are for saturation biopsies. We 
rarely have saturation biopsies, but Medicare denies us the 88305 charge if 
more than 5 specimens. 

Other insurance companies tend to follow their lead  after a little time.  I 
believe reimbursement is 50-75% less for 5-20 biopsies, but don't quote me on 
that.  I expect we may see the end of saturation and multi-container prostate 
biopsies in the near future. 

Another issue for many outpatient labs in my area is that larger insurances are 
requiring their patients to go to large multinational labs.  We cannot accept 
many PPOs or Medicare replacement plans because of this. 

I feel it can be a disservice to the patient because they do not get the same 
personal, local service with good turn around times. Even my insurance requires 
me to go to one of these labs where I feel inconvenienced and frustrated at the 
wait time required to submit my sample and get results to my physician. 

On Oct 31, 2012, at 8:17 AM, Webster, Thomas S. twebs...@crh.org wrote:

 Here is what CAP has on their website about the issue.
 Only the TC of 88305 is being discussed for 2013. We should know fairly soon 
 the decision.
 More codes have been flagged as overvalued as well that could be cut for 2014 
 (PC and TC at this point).
 
 http://www.cap.org/apps/docs/advocacy/advocacy_issues/revaluation.pdf
 
 
 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use of 
 the intended recipient(s) and may contain confidential and privileged 
 information. You may NOT use, disclose, copy or disseminate this 
 information.  If you are