[Histonet] HSV

[Blazek, Linda via Histonet]

Dear all,

Is there anyone out there that has a good HSV control block that they could 
spare?  I'm sorry but I don't have anything to trade since we only have small 
biopsy specimens.

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

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[Histonet] CMV

[Blazek, Linda via Histonet]

Does anyone have a good CMV control block they would be willing to share?
Thanks

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



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[Histonet] job opening

[Blazek, Linda via Histonet]

We have a Histotech position open in a GI lab located outside Dayton Ohio. This 
position is Monday - Friday day shift position.  We are a growing company that 
has just opened a brand new facility, with windows so you can actually see 
outside!  You must be HT (ASCP) certified or eligible and have a minimum of one 
year of experience as a Histology Technician. We work hard to encourage team 
values, open honest communication and a system of continuous quality 
improvement.
For consideration for this position, please send a cover letter and your resume 
directly to me at 
lbla...@digestivespecialists.com.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



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Re: [Histonet] Cameras in the Laboratory

[Blazek, Linda via Histonet]

If you could share the responses you get I'd appreciate it.  
We just built a new facility.  There are cameras everywhere.  They are in the 
lab and I hate them but you soon forget that they are there.  They cover almost 
all of the lab.  There is a small area that isn't covered and we call it the 
personal scratching area.  LOL .  My office does not have a camera in it.  
Linda

-Original Message-
From: Pratt, Caroline via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, January 22, 2019 3:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cameras in the Laboratory

If anyone is using cameras in the laboratory.  Can you share your experience 
with me and the placement/use/effectiveness, etc?  
caroline.pr...@uphs.upenn.edu.  Thank you!


Caroline M. Pratt, MBA
Practice Administrator Dermpath
3020 Market Street, Ste 201
Philadelphia, PA  19104
Phone 215-349-8178
Cell 610-800-1381
Fax 215-662-6150

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Re: [Histonet] Eosin on processor for biopsies

[Blazek, Linda via Histonet]

We have put eosin in the last alcohol on the processor for years.  It stays in 
the esophageal biopsies just fine.  
Linda


-Original Message-
From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, January 10, 2019 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin on processor for biopsies

Gareth,

reguarding the eosin biopsy post on the histo net. Things you might want to 
considered looking at, I cannot say for sure because I don't know your 
processing solutions/schedule:

Most Eosins are alcohol soluable, by putting the eosin in your formalin most of 
it will wash out during processing. Many labs put the Eosin in the last alcohol.

Hematoxylin is typically water soluable, if you put it in formailin processing 
usually removes water if the formailin is followed by alcohols.

I hope this helps,

Cassie


From: Gareth Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 10 January 2019 9:15 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinion on dye on biopsies

So, I work in a small GI lab, and I put Eosin in my first formalin on my 
processor.  My biopsies are very small and this helps, somewhat, to see the 
specimens for embedding and cutting.  But, unfortunately, the esophagus tissues 
do not absorb the eosin much.  Anyway, the hospital lab I work, part-time, in 
has started using hematoxylin to help see their biopsies.  I happen to embed 
there and I think it just makes a big mess and the tissue does not absorb much 
of the stain.
What are other labs doing to aid in making their small biopsies easier to see?  
What are pros and cons to doing this, in your opinion?
Thanks!

--
*Ms. Gareth B. Davis*, B.S., HT, QIHC  (ASCP)cm Yuma Gastroenterology Yuma, AZ 
85364
928-248-5259
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[Histonet] Job opening Dayton Oh

[Blazek, Linda via Histonet]

We're looking for a stand out Histology Technician.  This position is Monday - 
Friday day shift position.  We are a growing company with a brand new facility. 
To be successful in this job you must be focused, methodical, precise and able 
to work collaboratively with a variety of groups.  You must be Certified or 
eligible for Board of Certification (BOC) by the American Society of Clinical 
Pathologists (ASCP) and have a minimum of one year of experience as a Histology 
Technician with competency in the areas of embedding, microtomy and special 
stains and with a basic knowledge of immunohistochemistry. You must be able to 
work as a team that values open and honest communication, embraces challenge, 
innovation and continuous quality improvement, works hard, has fun and displays 
a can-do attitude.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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[Histonet] job opening

[Blazek, Linda via Histonet]

We have an opening for a Histology Technician. This position is Monday - Friday 
day shift position.  We are a growing company that has just opened a brand new 
facility, with windows!  You must be Certified or eligible for Board of 
Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and 
have a minimum of one year of experience as a Histology Technician with 
competency in the areas of processing, embedding, microtomy and with a basic 
knowledge of immunohistochemistry.  We only do a minimum number of special 
stains.  We work hard to encourage team values, open honest communication and a 
system of continuous quality improvement.
For consideration for this position, please send a cover letter and your resume 
to 
dsiemploym...@digestivespecialists.com
 and place your name on the subject line of the email, or contact me directly.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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[Histonet] job opening

[Blazek, Linda via Histonet]

We're looking for a Histology Technician, who will prepare histology slides 
from tissue sections for microscopic examination by staff pathologists.  This 
position is Monday - Friday day shift position.  We are a growing company that 
has just opened a brand new facility, with windows!  You must be Certified or 
eligible for Board of Certification (BOC) by the American Society of Clinical 
Pathologists (ASCP) and have a minimum of one year of experience as a Histology 
Technician with competency in the areas of processing, embedding, microtomy and 
a minimum number of special stains and with a basic knowledge of 
immunohistochemistry.  We work hard to encourage team values, open honest 
communication and a system of continuous quality improvement.
For consideration for this position, please send a cover letter and your resume 
to 
dsiemploym...@digestivespecialists.com
 and place your name on the subject line of the email.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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Re: [Histonet] Quality Measure for Pathology

[Blazek, Linda via Histonet]

Gudrun,
Now you're making me think!  I do have a cut-off.  It's at 95%.  I do keep 
records of the percentages of specimens that do not fall into the range.  But 
as to the process of solving the problem it's just counseling.  As we have only 
hit that cut-off once in the past three years I really haven't thought it out 
farther than that.  

Linda

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, June 13, 2018 11:55 AM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Quality Measure for Pathology

Linda,
that's exactly what we do here.
I wondered, if you fixed a cut-off. Because in quality-audits they often
asked the "what do you if.."-questions. 
for example: "There is an increase in the number of discrepancies in this
special month. The rate is over your defined aim. What did you do to fix the
problem? Do you keep records about the process of solving the problem?"

I was just curious, if you go further than the counting of events. 
In daily work we often know the causes of mistakes, and very often we know,
that a certain amount of mistakes can't be avoided, although everyone tries
to give his/her best. 
I think QM should help to discover the special events or circumstances, that
raise the number of mistakes above the "normal" level. I was curious, if you
defined this "normal" level for your lab.

Kind regards
Gudrun


-Ursprüngliche Nachricht-
Von: Blazek, Linda [mailto:lbla...@digestivespecialists.com] 
Gesendet: Mittwoch, 13. Juni 2018 14:38
An: Gudrun Lang
Cc: histonet@lists.utsouthwestern.edu
Betreff: RE: [Histonet] Quality Measure for Pathology

Gudrun,
Our lab is 95% biopsy specimens.  Therefor the number of pieces counted at
gross is written on the side of the cassette so that at the time of
embedding the tech knows what should be present.  That helps in being sure
there isn't a piece stuck to the lid or forceps, or popped out when opening
the cassette.  It's really more of a Quality Assurance measure than tracking
a discrepancy.  I do watch how frequent there is a discrepancy though and if
there seems to be a pattern will initiate a review of processes for both the
grossing person and the embedding person.  
What we see most is the gross will say one piece and there will actually be
multiple tiny pieces or two pieces will be three at embedding.  As all staff
is aware that we are following this they tend to be much more precise in
counting at grossing and embedding.  
Linda

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, June 13, 2018 4:19 AM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Quality Measure for Pathology

Linda,
How do you handle this key figure of discrepancies? Have you set a cut-off
(per person, per day) for any consequencies? What are the
consequencies, besides repeated training?

thanks
Gudrun

-Ursprüngliche Nachricht-
Von: Blazek, Linda via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Dienstag, 12. Juni 2018 18:55
An: Normington Lacy; Amy Self
Cc: histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Quality Measure for Pathology

Also a discrepancy between the number of pieces that are dictated at gross
as being in a cassette compared to what is found in the
cassette at embedding.  You can record the number of blocks that need
re-embedded and the number of slide recuts needed a month. 

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Normington Lacy via Histonet
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 9:34 AM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Quality Measure for Pathology

Some ideas:

Proficiency Testing
Corrected Results (Addendums/Amendment) volume
Turnaround Time Monitoring
Frozen Section TAT (critical call)
Rejected Tests (empty bottles, etc)


-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 8:18 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Quality Measure for Pathology

WARNING: This email appears to have originated outside of the UW Health
email system.
DO NOT CLICK on links or attachments unless you recognize the sender and
know the content is safe.




Good Morning,

I am looking for ideas/suggestions for QM Histology/Pathology.  My QM
director wants me to "measure" something that I can place on
the lab dashboard.

Thanks in advance for your help..

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through bett

Re: [Histonet] Quality Measure for Pathology

[Blazek, Linda via Histonet]

Gudrun,
Our lab is 95% biopsy specimens.  Therefor the number of pieces counted at 
gross is written on the side of the cassette so that at the time of embedding 
the tech knows what should be present.  That helps in being sure there isn't a 
piece stuck to the lid or forceps, or popped out when opening the cassette.  
It's really more of a Quality Assurance measure than tracking a discrepancy.  I 
do watch how frequent there is a discrepancy though and if there seems to be a 
pattern will initiate a review of processes for both the grossing person and 
the embedding person.  
What we see most is the gross will say one piece and there will actually be 
multiple tiny pieces or two pieces will be three at embedding.  As all staff is 
aware that we are following this they tend to be much more precise in counting 
at grossing and embedding.  
Linda

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, June 13, 2018 4:19 AM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Quality Measure for Pathology

Linda,
How do you handle this key figure of discrepancies? Have you set a cut-off (per 
person, per day) for any consequencies? What are the
consequencies, besides repeated training?

thanks
Gudrun

-Ursprüngliche Nachricht-
Von: Blazek, Linda via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Dienstag, 12. Juni 2018 18:55
An: Normington Lacy; Amy Self
Cc: histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Quality Measure for Pathology

Also a discrepancy between the number of pieces that are dictated at gross as 
being in a cassette compared to what is found in the
cassette at embedding.  You can record the number of blocks that need 
re-embedded and the number of slide recuts needed a month. 

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Normington Lacy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 9:34 AM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Quality Measure for Pathology

Some ideas:

Proficiency Testing
Corrected Results (Addendums/Amendment) volume
Turnaround Time Monitoring
Frozen Section TAT (critical call)
Rejected Tests (empty bottles, etc)


-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 8:18 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Quality Measure for Pathology

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




Good Morning,

I am looking for ideas/suggestions for QM Histology/Pathology.  My QM director 
wants me to "measure" something that I can place on
the lab dashboard.

Thanks in advance for your help..

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through better health.
NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this
message is not the intended recipient, or an employee or agent responsible for 
delivering this message to the intended recipient,
you are hereby notified that any dissemination, distribution or copying of this 
communication is strictly prohibited. If you have
received this communication in error, please notify us immediately by replying 
to this message and deleting it from your computer.
Thank you.
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Re: [Histonet] QM Dashboard

[Blazek, Linda via Histonet]

Terri,
I completely agree with you on the recut issue but I separate the recuts by 
Quality Issue and just a deeper section.  If a section is folded, has chatter 
or some other quality issue then I think it needs addressed as a Quality 
Management issue.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 2:21 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] QM Dashboard

We use: 
1.  Report TAT
2.  Discrepant pathology reports
a.  Internal vs External report correlation
b.  Frozen section vs Final diagnosis correlation
c.  Amended reports
I liked the idea of piece count discrepancy or even poorly processed blocks.  I 
never recommend using "recuts" as a QM because that is more often a product of 
the pathologists' preference or desire to see more tissue and does not reflect 
the quality of the sections (unless it is an incomplete facing issue).  This 
can quickly become punitive for your department because this information is not 
presented to people who understand pathology.
My 2 cents. Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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Re: [Histonet] Quality Measure for Pathology

[Blazek, Linda via Histonet]

Also a discrepancy between the number of pieces that are dictated at gross as 
being in a cassette compared to what is found in the cassette at embedding.  
You can record the number of blocks that need re-embedded and the number of 
slide recuts needed a month. 

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Normington Lacy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 9:34 AM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Quality Measure for Pathology

Some ideas:

Proficiency Testing
Corrected Results (Addendums/Amendment) volume
Turnaround Time Monitoring
Frozen Section TAT (critical call)
Rejected Tests (empty bottles, etc)


-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 8:18 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Quality Measure for Pathology

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




Good Morning,

I am looking for ideas/suggestions for QM Histology/Pathology.  My QM director 
wants me to "measure" something that I can place on the lab dashboard.

Thanks in advance for your help..

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through better health.
NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this message is not the intended 
recipient, or an employee or agent responsible for delivering this message to 
the intended recipient, you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. If you 
have received this communication in error, please notify us immediately by 
replying to this message and deleting it from your computer.
Thank you.
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Re: [Histonet] Slide printer and cassette label

[Blazek, Linda via Histonet]

Karen,

We print an average of 150 blocks a day and 250+ slides a day.  We have 2 
cassette printers and 1 slide printer.  We have had them for several years now. 
 They are Primera printers purchased from Creative Waste Solutions. 
https://cwsincorp.com/
We have had excellent results with them both in functionality and service.  
Also they can connect to your LIS if you want very easily without needing an 
expensive interface.

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, March 14, 2018 8:50 AM
To: Histonet (E-mail)
Subject: [Histonet] Slide printer and cassette label

Good Morning,
I am looking for suggestions for a slide printer and cassette labeler for a 
smaller lab.  Any suggestions.   I need it with the two patient identifier.

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

Caution:  This email message, including all content and attachments, is 
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received this communication in error, please notify us  immediately by reply 
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Re: [Histonet] Slide labels or slide labeler

[Blazek, Linda via Histonet]

I've used the Primera slide printer since it came out.  I like it a lot.  It 
needs it's maintenance kept up like anything else but other than cleaning the 
rollers there is very little else that needs cleaning.  

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Victor via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, March 07, 2018 5:59 PM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide labels or slide labeler

James,

I totally agree with your assessment of the Slidemate printers. Have not had 
any experience with the newer model. 
Applying slide labels shouldn’t be an issue when dealing with one block and 
slide(s) at a time.  Some of this could depend upon your software available to 
you. Our system generated just the labels needed for the block you were 
cutting, on demand. You should be able to get a nice Zebra printer for under 
$700.

Another of our labs used the new Sakura/Premera printer and really like it.

Victor

Sent from Mail for Windows 10

From: Vickroy, James via Histonet
Sent: Wednesday, March 7, 2018 2:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide labels or slide labeler

We have used the older version Slidemate slide labelers from Thermofisher for 
the last three years.   I think putting the patient information directly on the 
slide is preferred however the wear and tear on slide printers means higher 
maintenance costs and more down time.  Thermofisher has a newer version of the 
Slidemate now that has been designed so that there is less wear and tear on the 
machines.   Currently when one of the old labelers breaks down we have to send 
the instrument out and the cost is generally 1000 - 1500 for repair each time.  
  It also seems that the older machines have to be sent in at least every other 
year.  Our older machines have been sent in almost yearly.

We are considering switching to a slide label making system by General Data.   
One of the downsides is that we have to apply the label to each slide so 
therefore we introduce another step where the slides could be mislabeled.   The 
upside is that equipment costs are much less.   A new thermal label printer 
runs around 700.00 where as the instrument that imprints the slide label on the 
slide runs between 12000 - 15000 per unit.  We need three units.
Thermo also offers a lease system.What I need to know is what has been the 
experience with the new Slidemate AS printers?   What kind of maintenance 
issues have you encountered? Finally please let me know your latest experiences 
with both slide label printers versus slide labeling printers?

Thanks for your input.

Jim


Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

[Blazek, Linda via Histonet]

I really take exception to those finding fault with this book!  Maybe another 
word other than hack would have made some people feel better but we all know 
the intent.  Stating that "many of the fixes or secrets described are because 
some hack failed to do her/his job at an earlier step in the process" is 
totally incorrect.  Very few of the "hacks" has anything to do with someone 
failing to do their job.  I think this books intent was to help new people in 
the field of histology.  It has been a great form of dialog between our new 
tech and the older techs.  It has served a very good purpose.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc

-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, January 16, 2018 11:11 AM
To: Histonet
Subject: Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

Kind of in that same vein, but not to denigrate the book ( I have not seen it), 
I try to impress on people that "enabling" a poor process via "workarounds" or 
"shortcuts" does nothing to solve the original problem - it just prolongs the 
problem and peoples frustration with the problem. A much better path is to 
figure out why people feel the need for a workaround and then solve that 
problem. In other words, if a workaround is needed, then you have a problem 
that needs solving!

So now I have people come to me  saying  "I think this thing we do is a 
workaround for another problem." That is the kind of observation you like to 
hear.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Steve McClain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 16, 2018 4:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

I purchased the book and applaud the effort because there is some decent 
information.  However, the term hack is a poor choice for histology and many of 
the fixes or secrets described are because some hack failed to do her/his job 
at an earlier step in the process. (Definition of hack. transitive verb. 1 a : 
to cut or sever with repeated irregular or unskillful blows. b : to cut or 
shape by or as if by crude or ruthless strokes. As a noun it is used to mean a 
mediocre performer or worker; tiresome drudge.)

Some methods, while useful in some settings, have important cons not listed, 
cons which may be counterproductive. For example using Mercurochrome or Eosin 
to mark tissue may preclude further testing with fluorescent endpoints, such as 
FISH.  Plus if you really want to use Eosin to mark the dermis, it is far 
easier to add used Eosin to one alcohol in the tissue processor. That gives a 
visible indicator of carryover, indicating need to change or rotate solutions.

Other methods seem (to me) like workarounds or Band-Aids for Labs w poor 
grossing, poor processing or poor reagents or poor technique or poor method 
choices, eg, 2.14 describes a situation where an incompetent grosser truly 
hacks or crudely cuts into unfixed tissue yielding too thick a slice. The real 
solution is to fix the tissue before slicing. Poor fixation results in poor 
processing and poor sectioning and poor staining reactions.

For another example, Cassette sponges offer few advantages, while folding lens 
paper allows the grossers to see through the paper and know all pieces are 
inside before closing the cassette lid.  The tissue does not stick to it, and 
small flakes can be scraped from the lens paper at embedding. Last during 
folding, the forceps can be cleaned at grossing and during unfolding, forceps 
may be cleaned w the paper after embedding.  Sponges also result in greater 
solution carryover.

Several colloquial naming conventions, eg, chamber saver 2.16 for 
underprocessed tissue may be memorable to some readers, yet seem  are odd to me.

This 2.16 method is an especially useful technique which may also be done to 
extend paraffin time, whenever poor sectioning due to poor processing is 
encountered at the microtome.
Variation 1 Place the block back into the proper sized mold and return to the 
heated side of the embedding center for an hour to extend processing 
(reprocessing). Then remove the old paraffin from the mold w a plastic pipette, 
 then re-embed, replacing the paraffin w new.
Variation 2 for outside blocks we routinely replace an unknown paraffin from 
another lab by melting in a mold, and 'reprocess' in our (blue ribbon) paraffin 
for 1 hr in a mold in the embedding center then re-embed.

Good first effort, yet this book could be improved by a good editor, by more 
collaborators, by illustrations, and the addition of variations or other uses 
as described above for 2.16.
The font size and format will cause many readers to suffer

Re: [Histonet] Histology Hacks - the book

[Blazek, Linda via Histonet]

I agree with Terri!  We have a newly registered tech and several of us "oldies" 
and it started a great dialog on tips we have run across in the years.  The 
book has some things we had forgotten too!  We all have been enjoying reading 
and adding little comments.  Since we are a GI lab it was great to have some of 
the other hints that our new tech hasn't had much exposure to.  
Good job Michael!

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, January 12, 2018 11:06 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Histology Hacks - the book

I just wanted to share a quick review of the new book "Histology Hacks", by 
Michael Backhus.  It is the kind of book that brings together all of the little 
tidbits that a tech picks up along a lifetime of working and networking with 
others.  It is well organized and the information is presented with the pros 
and cons of each hint.  The big bonus was that by flipping through the book in 
my department, it started a conversation on techniques and experiences with 
similar tips.  Anything that gets your Histology team excitedly talking about 
their profession is well worth the relatively small investment.  It would make 
a great staff present for Lab Week.  I don't know Michael, but kudos!  Check it 
out on Amazon.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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[Histonet] counters

[Blazek, Linda via Histonet]

Good morning.  Does anyone have a picture of a curved counter top for use with 
the microtome?  I thought I had one but can't seem to find it.

Thanks!

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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Re: [Histonet] cassette printers - multi color cassettes and network ready-

[Blazek, Linda via Histonet]

Cheryl,
We use the Primera printer and have had great success with it.  It's small and 
reliable.  You can get it from http://cwsincorp.com/

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Cheryl via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 15, 2017 3:57 PM
To: Histonet
Subject: [Histonet] cassette printers - multi color cassettes and network ready-

What are you using for your cassette printer?  The Leica IP-C is a familiar 
model but it is SO BiG.
We need 3-5 colors of cassettes, and it has to network so we can have multiple 
users.  We print two lines (number and name) with hopes to go to full bar 
coding in the near future. Looking for any/all input - vendors welcome, too. 
Cheryl Kerry, HT(ASCP) ADG pathologycke...@adgpath.com 
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Re: [Histonet] looking for a slider printer

[Blazek, Linda via Histonet]

Blanca,

We have had the  Primera Slide printer for several years now. It's a great 
small footprint printer and easily connects to our LIS.  Check with Creative 
Waste Solutions.  http://cwsincorp.com/
They sell them.  
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, April 21, 2017 12:09 PM
To: 
Subject: [Histonet] looking for a slider printer

Dear Histonets!!!
We are looking to buy a slide printer for a low cost, durable and easy to work. 
W are a very small lab, we don't' need anything fancy. If you know somebody or 
have a good experience with one please let me know.


Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] solvent recyclers

[Blazek, Linda via Histonet]

I have had the recycler from CBG for years and it's been a great workhorse!


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, March 23, 2017 11:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] solvent recyclers

Hi everyone,

We are looking into getting a new solvent recycler to recycle our xylene and 
alcohol, any recommendations?

Thanks!



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Re: [Histonet] Bob Richmond

[Blazek, Linda via Histonet]

Happy retirement Dr. Richmond!  Just don't retire from the histonet!  We need 
you.  I too have a wonderful Samurai collection that someday I'll hand down to 
a "newbie" that will just scratch their head and say "really"
Your advice and humor have been appreciated (and collected) more than you know!
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Gagnon, Eric via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, March 13, 2017 10:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bob Richmond

Happy 'retirement', Bob. Old pathologists never retire. They are just 
glassy-eyed!



Appreciative of your many years of pragmatic, might I say homespun, advice and 
counsel on this list. Many of your posts have been printed off and retained in 
my Big Binder of Histo-Truth.



Enjoy!



Eric Gagnon

Histology Laboratory

Kingston General Hospital,

Kingston, Ontario, Canada.





>The old Samurai Pathologist - now retiring at 78 - thanks the many
histotechnologists who've kept him out of hot water the past 52 years.

Too bad we can't have more kids in search of a career reading Histonet - or at 
least, the Help Desperately Needed notices!

Bob Richmond
Samurai Pathologist
Maryville TN

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Re: [Histonet] cassette printer additional request

[Blazek, Linda via Histonet]

We have the Primera Signature Cassette printer and like it a lot.  Support is 
excellent but we haven't needed it much.  Set up is easy.  Printing is very 
good.  We also have their slide printer that is interfaced with our LIS.  It's 
sold by Creative Waste Solutions.
 http://cwsincorp.com/
If you'd like any questions answered feel free to contact me.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Eileen Akemi Allison via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, March 01, 2017 8:31 AM
To: Histonet
Subject: [Histonet] cassette printer additional request

Hi Again:

I forgot to mention I am open to investigate other Cassette Printers other than 
the General Data Printer… AlOf course, price, and dependability is a big 
factor. Would love to hear your comments, good, bad or indifferent...

Thanks in again!

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 

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[Histonet] Excelsior AS

[Blazek, Linda via Histonet]

Does anyone have any experience using this processor?  Could you share with me 
the good and/or bad you have experienced?
Thanks in advance!  :)
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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Re: [Histonet] (no subject)

[Blazek, Linda via Histonet]

Try the BioCare antibody.  It's excellent.

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, December 01, 2016 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Since, Cell Marque H. Pylori is not available,  can some one give a comparable 
antibody to order?  Thanks in advance


Nirmala Srishan








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for patient care, clinical performance and workplace excellence.
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[Histonet] new lab

[Blazek, Linda via Histonet]

Hi all,

It's finally official.  We will be building a new facility with ground breaking 
in the spring!  I have seen the drawings for the designated area in the new 
building for the histology lab.  Now I could use any suggestions that you all 
may have in designing a new lab.

Thanks in advance!
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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[Histonet] rotomat

[Blazek, Linda via Histonet]

Good morning,
Does anyone have any firsthand knowledge of the Hanel Rotomat slide storage 
system?
Thanks,

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


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[Histonet] expiration dates

[Blazek, Linda via Histonet]

Does anyone see an expiration date printed on their can of Freeze Spray?
I was told by the company I purchase mine from that it was not needed because 
it was "not a medical device".

Thanks
Linda

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Re: [Histonet] Job opportunity in Colorado-

[Blazek, Linda via Histonet]

I'm sorry, I can't resist.  I guess it's just too close to vacation time...  
How strong do you have to be?

-Original Message-
From: Taylor Rinaldi via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 03, 2015 12:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Job opportunity in Colorado-

Hello all! 

 

My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We 
specialize specifically in laboratory recruiting all over the United States for 
many hospitals and reference laboratories. We just received a new order for a 
great opportunity with a well-known hospital located in Colorado. We are 
recruiting for a strong Histotechnician/Grosser for their lab. This position is 
a dayshift, fulltime, permanent opportunity. ASCP certification preferred. If 
you may be interested in this or any of our other opportunities, please reach 
out to me for immediate consideration.

 

Thank you in advance!

 

Taylor Rinaldi

Recruiting Manager

Prometheus Healthcare 

Office (301) 693-9057

  tay...@prometheushealthcare.com

 

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Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

[Blazek, Linda]

Dear Mr. Cantankerous, :)

So how would you gross a colon biopsy that was <0.1 cm and probably mucus?  

-Original Message-
From: Steve McClain [mailto:ste...@mcclainlab.com] 
Sent: Wednesday, July 08, 2015 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

My goodness 7% tissue loss is ridiculous.
What fool published that?

A 0.8% "loss of tissue" is beyond me.
Would any one advertise in the local paper that your center of excellence lab 
loses nearly 1% of your specimens?

"possibly too small to survive processing" is in my opinion, a self-fulfilling 
prophesy, one to be eschewed and denounced, or beaten out of the grosser who 
dares use that description for it is an expectation of failure.
That grosser would not last a week around me.
I would walk them to the train station and ask them not to return.

I felt less cantankerous before I read that one. 
Steve A. McClain, MD
631 361 4000


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Re: [Histonet] IHC repeat rate

[Blazek, Linda]

I would think that it would really depend on if it is a repeat due to an 
instrument problem or a human error.  I think that if a vendor says that a 5% 
repeat rate is acceptable for their instrument I would look for another vendor. 

-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Thursday, June 25, 2015 3:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC repeat rate

I once had a vendor tell me that 5% was an acceptable rate.  I am more inclined 
to use 2%

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
   
4. IHC repeat rates (Bitting, Angela K.)
Message: 4
Date: Thu, 25 Jun 2015 15:08:46 +
From: "Bitting, Angela K." 
Subject: [Histonet] IHC repeat rates

What does this group feel is an acceptable repeat rate for automated IHC 
staining??
Thanks for your responses in advance.
Angie
***


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Re: [Histonet] de-waxing protocol on instruments just fact finding

[Blazek, Linda]

Rachel,

We dewax off line.  We use the same protocol that we do for any other staining; 
3 min in  3 changes of xylene (we use a xylene substitute though), 3 min in 2 
changes of 100% alcohol and one 3 min in 95%.  
We use BioCare's IntelliPath immuno stainer.  It is a completely open system.  
All of the sales reps, support techs, and service tech have always been very 
open and willing to help in any way to provide information and support for this 
stainer.  If by dewaxing you are also referring to off line antigen retrieval, 
we use the pressure cooker that BioCare has.  You can use any retrieval 
solution you choose with it.  If you need to use two different retrieval 
solutions you can fill your containers with two different retrieval reagents.  
I don't find that there is any difference in doing off line retrieval than on 
line retrieval.  
If I can answer any other questions please feel free to contact me.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Rachel M Gonzalez [mailto:rac...@gbi-inc.com] 
Sent: Monday, June 22, 2015 5:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] de-waxing protocol on instruments just fact finding

Hi

Just beginning to look at instruments DAKO Ventana Leica. We may not have the 
budget but if we do However talking to the sales reps have been challenging 
as everything is proprietary.

This is for research consideration so we will use an open instrument? Which 
instrument do you like better open?

Does  manual dewaxing affect the staining? Every rep says yes... but does it?

What is the time for dewaxing when you start the instrument? What are the 
number of steps in the protocol? What is the waste generation like more or less 
than manual?

Thanks for your help.

Rachel
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[Histonet] plants in the lab

[Blazek, Linda]

Happy Friday all!

Does anyone have documentation of the benefit of having plants in the lab?  I 
know this was discusses quite a while ago but I can't find references for it.  
Any help would be appreciated.

Thanks,
Linda
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[Histonet] RE: Nuclear "Artifact"

[Blazek, Linda]

The first place I would look is to what may be happening before they reach me.  
If it's only one site with an issue, it sounds more like an issue at collection.
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa
Sent: Tuesday, April 21, 2015 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nuclear "Artifact"

Hi HistoNetters:
I have run into quite a problem.  My lab currently processes all tissue types 
from 3 different sites.  Recently, we have been getting complaints from one of 
the sites that the biopsies have a nuclear "artifact".  It is described as 
"washed out" or poor to no nuclear detail.  Pictures have been uploaded 
(Nuclear "Artifact").  The Medical Director at said site is convinced that a 
processor error is occurring.  Our site is not seeing this on any of our 
slides.  Biopsies from all three sites are processed and embed together.  We 
have done all trouble shooting that we can think of.  Leica service has come to 
inspect our Peloris processor and all areas checked out as functioning 
properly.  The problem is not consistent daily.  Seems to be worst toward the 
end of the week.
We have been running the same processing protocol, staining protocol and 
cutting protocols for years now.  This problem has just developed over the last 
2 months.  Any ideas, no matter how far-fetched, would be greatly appreciated 
at this point.

Lisa Roy, HT(ASCP)
Histology Supervisor
LabCorp at St. Vincent Hospital
123 Summer St
Worcester, MA
(508)363-9420

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RE: [Histonet] Orange peel for GMS

[Blazek, Linda]

Our blue cheese GMS would make you never want to eat it again!

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Friday, April 17, 2015 11:21 AM
To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

We just tried a moldy  strawberry. It was loaded with fungus!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Friday, April 17, 2015 7:11 AM
To: 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

I got a nice GMS control from some moldy chicken in my fridge. I am also trying 
the onion. I haven't stained the onion yet but it actually cut beautifully so I 
have high hopes. I let you know how it turns out. Have a nice weekend!

Jessica Piche, HT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of T Williams
Sent: Thursday, April 16, 2015 9:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Orange peel for GMS

I tried using a moldy orange peel as a GMS control, but the staining did not 
work.  I ran it in a 10 hour processing protocol.  Should I try something 
different?  Any suggestions would be extremely helpful - we're desperate for a 
GMS control.

Thanks,
T. Williams, HTL (ASCP), QIHC
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RE: [Histonet] BS in Histotechnology

[Blazek, Linda]

Fortunately times have changed.  The person I replaced in the late 70's early 
80's had been brought in from the hospital laundry and was "trained".  They 
were still pouring embedding molds then.  She did a beautiful job at cutting 
and staining H&E slides and 2 or 3 specials but that was all there was to do.  
This world has come a lot farther than those days of the 80's and 70's.  We 
have grown into fully capable labs that don't have to send work out to the big 
reference centers to have tests done.  I remember a class with Lee Luna when he 
said "We have to excel and learn these immuno procedures or the MT's were going 
to take them away from us."  We learned and they didn't!  We may not be 
recognized at the level with MT's but we are slowly changing and getting there. 
 At least now an associates is required and CEU's are required.  I think that's 
progress.  If you're looked down on in your present position move on!  It's not 
worth it.
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Tuesday, March 24, 2015 1:53 PM
To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: RE: [Histonet] BS in Histotechnology

That was nicer than the pathologist who told me years ago, "any monkey could be 
trained to do my job".  I basically did not take the job I was interviewing for 
at the time.  At least the next interview went a lot better and I did take the 
job.

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 24, 2015 12:30 PM
To: Sue; Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: RE: [Histonet] BS in Histotechnology

I agree, BUT>as long as many pathologists think you can teach any 
trained dog how to section histology will never have the recognition those of 
us that have studied and trained deserve.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 24, 2015 12:59 PM
To: Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: Re: [Histonet] BS in Histotechnology

This is a fight that we continue to have with hospital administration.  In my 
opinion histologists are just as important and needed as MT.  Even though there 
is an increase in automation in pathology the hands on of a histologists is 
most important.  The fact that hospital still consider a lower entry job is the 
reason there are not more of us.  It is quite frustrating. 
  
Sue 
TJUH 
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[Histonet] job opening

[Blazek, Linda]

I'm posting this for Matt .  And it is a great place to work!
Linda


Hey All
We have a full time position now open at the Dayton Children's Hospital. This 
is a small lab, we process between 15 and 50 blocks per day. Pay will be 
determined according to experience. Must be certified or certified eligible 
with-in two years. Great place to work, little stress, great people and 
benefits. Follow the link to apply.

http://www.childrensdayton.org/cms/sitelet/job_postings/index.html

Click on Technical and Full Time


Matt Chase, HT (ASCP)
Supervisor of Pathology

Dayton Children's
One Children's Plaza
Dayton, Ohio 45404-1815
Office Number: 937-641-3000 Ext 8229
Fax Number: 937-641-5482
Email: cha...@childrensdayton.org
Website: childrensdayton.org

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RE: [Histonet] Controls:

[Blazek, Linda]

Look at one under the scope!  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy
Sent: Thursday, March 05, 2015 1:30 PM
To: James Watson; 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:

Do you mean using Slim Jims right out of the package, as is, for Gram controls? 
 What is in those things - sounds like a food safety issue!


-Nancy Stedman

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, March 05, 2015 1:01 PM
To: 'Jb'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls:

Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all 
the labs at AFIP.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Wednesday, March 04, 2015 1:05 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Controls:

Off the wall question, I have been told that slim jims (pepperoni stick) at the 
gas station can be processed and used as good gram controls. Has anyone done 
this and do they work for GMS also?

Thank you,

Sent from my iPhone
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[Histonet] RE: need light for flotation bath

[Blazek, Linda]

I found them at Lowe's.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Preiszner, 
Johanna
Sent: Monday, March 02, 2015 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] need light for flotation bath

Hi,

we have a Fisher Tissue Prep 135 flotation bath and the light died. The 
instrument is no longer manufactured and I cant find a source for the 
fluorescent light tube. There are multiple baths for sale on ebay but nobody 
sells the light alone.

Could someone help me out? Would something from Home Depo do?


Thanks,
Hanna




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RE: [Histonet] Receipt of specimens

[Blazek, Linda]

I actually use three different times for tracking since it means different 
things to different people.  I track collection to sign out, received in the 
lab to sign out, and report ready to sign out.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik
Sent: Tuesday, February 10, 2015 2:25 PM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Receipt of specimens

Tanya, you can probably put either date/time into the receive date/time prompt 
of your LIS.  It just depends on how you want to use that information.  For 
instance, perhaps you want to look at turn around times.
>From the lab's perspective, the 'clock' starts when the sample is 
>received
in the lab.  On the other  hand, from the physician's perspective, it starts at 
sample collection and from the organization's perspective the clock might start 
when the sample was put in the fridge.

If it was 'me', I'd probably put the date/time the sample was received in the 
lab and I'd make sure the other information was either scanned into the
case via paper or somehow noted somewhere else.   The reason for my thinking
is that those cases received off shift are going to skew your turnaround times 
if that's something you measure.

Bottom line, it just depends on what you're using that information for.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Monday, February 09, 2015 9:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Receipt of specimens

I am curious as to how everyone handles the receipt of their surgical, and 
Cytology specimens in to the lab? Especially on the off shift hours? Other 
clinical lab specimens are received directly in the computer in Specimen 
Processing when they come in the door from remote locations or via the tube 
system, then delivered to departments. During the day, specimens get dropped 
off directly to Pathology by the various departments collecting (ie. OR, 
Radiology, etc). On our off shifts, specimens get received by Specimen 
Processing(date/time stamped and initialed)  and put in the fridge for 
Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, 
for the "receive date", they go by the date the specimens are "received" (or 
unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen 
Processing received them in to the actually laboratory where the specimens are 
then "controlled".
Any input is appreciated! Thanks!
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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RE: [Histonet] RE: Cassette Labeler

[Blazek, Linda]

I've had mine longer than that and don't have a problem.  I have a feeling that 
you may need a bit of service on it.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge
Sent: Tuesday, January 27, 2015 1:11 PM
To: Helen Fedor
Cc: histonet@lists.utsouthwestern.edu; Debbie Granato
Subject: Re: [Histonet] RE: Cassette Labeler

I also have a Primera slide printer. It worked well at first but now it is
2 years old and prints very slowly. I can hand write up slides faster than they 
print. I will be in the market for a new slide printer soon and will not be 
going back to Primera.

-- 

Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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[Histonet] RE: Cassette Labeler

[Blazek, Linda]

I second that!  I love mine.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Tuesday, January 27, 2015 12:25 PM
To: Debbie Granato; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cassette Labeler

Hello, we recently purchased the Primera unit from Creative waste solutions.
http://www.cwsincorp.com/

we like it. I would be happy to discuss it further if you like.


Helen L. Fedor 

Oncology Tissue Services, Manager
Johns Hopkins University
411 N. Caroline St
Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231

410-614-1660

http://tmalab.jhmi.edu/






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Granato
Sent: Monday, January 26, 2015 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Labeler

Our lab is currently looking into purchasing a cassette and slide labeler.

We are a small lab and are looking for a stand- alone unit with the flexibility 
to be used with the computer or bar code scanner at a later date.

I would appreciate any feedback or suggestions for any model that may fulfill 
our needs.

Thank you,

Debbie Granato HT(ASCP)


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[Histonet] CCK testing

[Blazek, Linda]

Dear all,

If there is anyone that is doing crystal identification in bile fluid could you 
contact me off list?

Thanks,
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

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[Histonet] RE: Slide and cassette labelers

[Blazek, Linda]

You might also look at the Primera printers sold by Creative Waste Solutions.  
I have both the slide and cassette printer and like them both.  They are nice 
and compact.

http://cwsincorp.com/

Linda
Linda Blazek HT (ASCP)
Manager 
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Thursday, January 08, 2015 9:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and cassette labelers


Has anyone used the Tissue Tex "SmartWrite" Slide Printer and Cassette Printer? 
  These both use Thermal transfer.

I am also considering ThermoScientific, Leica, and General Data.

Any advice would be welcome.

Thanks

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
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taken on the contents of this information is strictly prohibited. If you have 
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[Histonet] control

[Blazek, Linda]

Hello all,
I was wondering if there is anyone that may have a positive HSV control that 
they would be willing to share.  I would greatly appreciate it!
Thanks in advance,
Linda

Linda Blazek HT (ASCP)
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

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[Histonet] RE: Angle of Wrist/Hands Using Microtome?

[Blazek, Linda]

My two cents...  # 2  Keep your wrist straight.
(you may get rich if you get enough 2 cents!)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Monday, December 08, 2014 4:33 PM
To: Smith, Denise; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome?

I think you want to keep your wrist straight so it would be 2.   Just my two 
cents

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Denise
Sent: Monday, December 08, 2014 1:53 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Angle of Wrist/Hands Using Microtome?

Hi all!

When I took HTL exam and there was a question on the exam that bothered me the 
most but I cannot find an exact answer for it, had to guess!  I'm very curious 
about your thoughts on this.  I have asked Histology Core and they weren't sure 
about it either - wanted to share it with you guys if you know the official 
answer for this!

The question was mainly about the angle of wrist/hands on the rotating 
microtome and the degree must be correct so it won't cause any stress on 
wrist/hands, it listed 4 different choices:


1.   30 degrees wrist down forward

2.   90 degrees straight without bending the wrist

3.   60 degrees wrist up backward

4.   60 or 90 degrees wrist down forward


(Please be gentle with me since I don't remember the EXACT numbers but those 
were close to numbers provided.)

Thank you!

Denise Smith


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RE: [Histonet] Re: Flames at embedding centers

[Blazek, Linda]

Since the break room was right across the hall from the histology lab, we use 
to clear off a counter to put all the food for our potlucks.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, November 19, 2014 2:04 PM
To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

 the good old days

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Wednesday, November 19, 2014 1:58 PM
To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

How about the person in the 1970's coverslipping with an open dish of xylene at 
AFIP and someone at the other end of the stain line decolorizing Brown and 
Brenn stains with acetone/ether in the sink; then the acetone/ether fumes 
migrating across the stain line to the cigarette and having the whole counter 
and wall catch fire.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Scientific 
Technical Leader II, Histology Tel    858-332-4647 Fax   858-812-1915 
jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, November 19, 2014 10:36 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Flames at embedding centers

Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes 
while hand-staining slides in rows of large Stender dishes, including a dish 
with 20% picric acid in acetone, used to remove formalin pigment (since 
buffering formalin wasn't permitted way back then).

Bob Richmond
Samurai Pathologist
Maryville TN
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RE: [Histonet] Re: Flames at embedding centers

[Blazek, Linda]

"It would be interesting to create an album of memoirs to share with our 
younger generations."

Calling Sally, calling Sally.  this is a good one for you to start!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Wednesday, November 19, 2014 1:42 PM
To: Bob Richmond; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Flames at embedding centers

It would be interesting to create an album of memoirs to share with our younger 
generations.


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Bob Richmond 
[rsrichm...@gmail.com]
Sent: Wednesday, November 19, 2014 1:36 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Flames at embedding centers

Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes 
while hand-staining slides in rows of large Stender dishes, including a dish 
with 20% picric acid in acetone, used to remove formalin pigment (since 
buffering formalin wasn't permitted way back then).

Bob Richmond
Samurai Pathologist
Maryville TN
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RE: [Histonet] Flames at embedding centers

[Blazek, Linda]

We're well preserved!


-Original Message-
From: Horn, Hazel V [mailto:hor...@archildrens.org] 
Sent: Tuesday, November 18, 2014 4:23 PM
To: 'Paula Pierce'; Blazek, Linda; Jay Lundgren; Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Flames at embedding centers

Yes, the good old days!And, we survived!

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children’s Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, November 18, 2014 3:14 PM
To: Blazek, Linda; Jay Lundgren; Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flames at embedding centers

Like! & ditto! Paula Pierce,BS,HTL(ASCP)HT President Excalibur Pathology, Inc. 
5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX 
www.excaliburpathology.com
  From: "Blazek, Linda" 
 To: Jay Lundgren ; Ludlow Patricia 
Cc: "histonet@lists.utsouthwestern.edu" 
 Sent: Tuesday, November 18, 2014 3:03 PM
 Subject: RE: [Histonet] Flames at embedding centers
   
Grinning and remembering the "good old days".  What's more fun is the look of 
horror on the faces of the young ones when they hear it!



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Tuesday, November 18, 2014 2:42 PM
To: Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flames at embedding centers

    Ahh, the good old days of walking into the lab and lighting all the Bunsen 
burners first thing in the morning.  I didn't have any hair on my knuckles for 
years.  Embedding with a Coke on the cold plate, and a smoke in the ashtray 
next to you, anyone?  Good times.

    Now we have to use forceps warmers and change forceps between specimens.  
If you get 3 or 4 pair of forceps, one will always be hot enough to use.  Also, 
there are embedding centers with heated forceps, which I love .
    Just remember to clean out the wells of the forceps warmers every day to 
prevent cross contamination.  Cotton applicator swabs work great for this.  And 
always keep a towel or gauze handy to wipe the tips of the forceps between 
specimens.  Forceps warmers unfortunately don't incinerate any stray tissue 
like a Bunsen burner did.

                      Sincerely,

                              Jay A. Lundgren, M.S., HTL (ASCP)

>
>
>
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RE: [Histonet] Flames at embedding centers

[Blazek, Linda]

Grinning and remembering the "good old days".  What's more fun is the look of 
horror on the faces of the young ones when they hear it!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
Sent: Tuesday, November 18, 2014 2:42 PM
To: Ludlow Patricia
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Flames at embedding centers

Ahh, the good old days of walking into the lab and lighting all the Bunsen 
burners first thing in the morning.  I didn't have any hair on my knuckles for 
years.  Embedding with a Coke on the cold plate, and a smoke in the ashtray 
next to you, anyone?  Good times.

 Now we have to use forceps warmers and change forceps between specimens.  
If you get 3 or 4 pair of forceps, one will always be hot enough to use.  Also, 
there are embedding centers with heated forceps, which I love .
 Just remember to clean out the wells of the forceps warmers every day to 
prevent cross contamination.  Cotton applicator swabs work great for this.  And 
always keep a towel or gauze handy to wipe the tips of the forceps between 
specimens.  Forceps warmers unfortunately don't incinerate any stray tissue 
like a Bunsen burner did.

   Sincerely,

   Jay A. Lundgren, M.S., HTL (ASCP)

>
>
>
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[Histonet] microwave

[Blazek, Linda]

Dear all,

Is there anyone out there doing microwave processing of GI biopsies?  What are 
you using and what are the pros and cons of what your choice of instruments has 
been?

Thanks in advance!
Linda



Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

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RE: [Histonet] Solvent Recyclers

[Blazek, Linda]

CBG 
 http://www.cbgtechnologies.com/

Completely dependable.  I've had mine for years with no problems at all.  It 
runs forever!
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann 
Deathridge
Sent: Thursday, October 30, 2014 2:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Solvent Recyclers

Any recommendations for solvent recyclers?  Due to space issues benchtop unit 
preferred.  "Dependability" more preferred.
 Pros and cons?  You may email if preferred.  Thanks in advance for responses
  
 Maryann .
  
  madeathri...@pastnashville.com  

  




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[Histonet] RE: H Pylori IHC

[Blazek, Linda]

You might find that the financial impact wouldn't be that great.  There is a 
reason they are doing an EGD, most commonly for gastritis which is a valid 
reason to look for H pylori.  There is a pretty standard list of ICD 9 codes 
that would trigger the justification for doing an H pylori.   Also, you're 
going to one of two things when you have a stomach biopsy.  You will either cut 
extra slides, (time and material there) or you will wait until its ordered 
(loss of TAT).  So, if you are going to do a retrospective study you need to 
include that in your review.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Monday, October 13, 2014 3:20 PM
To: 'wanda.sm...@hcahealthcare.com'; sbald...@mhhcc.org; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: H Pylori IHC

You might be able to get around this by doing the IHC on all of these as 
requested, but only charging for those which are indicated as being medically 
necessary. This could benefit your TAT, if that's the reason the pathologist 
wants these done routinely. The pathologist would also have to agree not to 
bill for the PC of this test in these cases also. You might want to do a 
retrospective study and determine if this was done what the financial impact 
would have been.
Good luck.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
wanda.sm...@hcahealthcare.com
Sent: Monday, October 13, 2014 2:49 PM
To: sbald...@mhhcc.org; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: H Pylori IHC

Kathy,
My understanding is CMS (Medicare) has ruled that h.Pylori stains cannot be a 
standing order.  The Pathologist must look at the H&E's first and deem the 
stain is needed based on the inflammation.


WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Baldwin, Kathy
Sent: Monday, October 13, 2014 2:36 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] FW: H Pylori IHC



Hi all
My pathologist would like our techs to do H pylori IHC stains on all stomach, 
GE Junction and esophagus BX's.  What I was wondering, if there were any 
indication in Regulations or anything that we couldn't do this?  Any advice 
would be greatly appreciated :)

S Kathy Baldwin
Histology/Cytology Supervisor
PH. 812-996-0210, Fax 812-996-0232
sbald...@mhhcc.org:)

"Christ's healing mission of compassion empowers us to be for others through 
Quality and Excellence"
Vision Statement:  We are committed to being the preferred health and wellness 
provider; transforming lives through faith based, compassionate care.


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RE: [Histonet] RE: New CPT Codes for IHC

[Blazek, Linda]

Joyce

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Wednesday, October 08, 2014 3:45 PM
To: 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New CPT Codes for IHC

I would like the correct answer for this.  I have always billed as Joyce 
states.  But a sister hospital says it's like Andrew's email.  Who is right?

Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas 
Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, October 06, 2014 3:41 PM
To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New CPT Codes for IHC

Sorry, I left that part out. We don't do them, so I just didn't think. 

I understand the 88343 codes to be for multiple stains on the same slide. The 
first is 88342 additional 88343 - e.g. PIN4.

So has that changed? 


Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email. 

-Original Message-
From: Andrew Horvath [mailto:ahorv...@cogipath.com]
Sent: Monday, October 06, 2014 3:36 PM
To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New CPT Codes for IHC

Actually, for non-Medicare if there are multiple stains performed on a block, 
where the initial CPT is 88342, any subsequent billing for stains on that block 
would actually be 88343 from the information I see.  

Please refer to the links below for more information...

http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf


https://www.aapc.com/memberarea/forums/showthread.php?t=101402



quote from the forum:

Quite and uproar about this in our office - and a lot of confusion. Go to page 
360 in this document to read Medicare's decision about this. (inactive link 
removed)


"The CPT Editorial Panel revised the existing immunohistochemistry code, CPT 
code 88342 and created a new add-on code 88343 for CY 2014. Current coding 
requirements only allow CPT code 88342 to be billed once per specimen for each 
antibody, but the revised CPT codes and descriptors would allow the reporting 
of multiple units for each slide and each block per antibody
(88342 for the first antibody and 88343 for subsequent antibodies). We believe 
that this coding would encourage overutilization by allowing multiple blocks 
and slides to be billed. 
To avoid this incentive, we are creating G0461 (Immunohistochemistry or 
immunocytochemistry, per specimen; first single or multiplex antibody stain) 
and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each 
additional single or multiplex antibody stain (List separately in addition to 
code for primary procedure)) to ensure that the services are only reported once 
for each antibody per specimen. We believe this will result in appropriate 
values for these services without creating incentives for overutilization."

Some of our docs are participating in conference call today with CAP to help 
clarify what this all means.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Friday, October 03, 2014 12:33 PM
To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: New CPT Codes for IHC

For non-Medicare it is per block - CPT code 88342.

For Medicare - per specimen. G0461 for the first antibody and G0462 for each 
additional different ab.

Best, j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the

RE: [Histonet] On the lighter side...

[Blazek, Linda]

41 in histology 32 registered.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Friday, August 08, 2014 6:00 AM
To: Douglas Porter; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

29 years. 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

  doug.por...@caplab.org 

  www.caplab.org   

 

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[Histonet] RE: Recycled or not? NO PHI

[Blazek, Linda]

I agree with Tom.  With the exception of self-inflicted issues we also have not 
had any issues with recycling our reagents.  We check each batch as it is 
recycled.  
We also don't have a problem with fumes.  (And our pathologists are fussy)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, 
Thomas
Sent: Thursday, June 26, 2014 10:34 AM
To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycled or not? NO PHI

We have never had an issue with either our recycled xylene or alcohol that was 
not self inflicted. When our system is running there are no fumes. 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Thursday, June 26, 2014 9:06 AM
To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycled or not?

While I can agree that recycling alcohol and xylene is both environmentally and 
economically advantageous, technically it's awful.  There's no way to make used 
alcohol and xylene as pure as it was originally.  There's also the issue of 
fumes from recycling a solvent.  The company I had used years ago swore that 
there were no fumes when using their machine but the personnel working in the 
laboratory would vigorously disagree. 

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Sanders, Jeanine 
(CDC/OID/NCEZID) 
Sent: Thursday, June 26, 2014 9:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recycled or not?

Morning All!

I have heard for years the general problems with using recycled alcohols on H&E 
stainers, but do the same problems occur when using recycled xylene?

Thanks!

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


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those of the author and do not necessarily represent those of LRGHealthcare.


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RE: [Histonet] Basement Lab

[Blazek, Linda]

The life of a basement lab rat isn't all bad!  We don't hear thunder storms.  
We are probably be safe from tornados, hurricanes and will have lots of company 
if one does come by.  Usually no one wants to go to the basement.  
There are no windows so there isn't any of the outside world distraction.  If 
it's a beautiful day you don't see it so you don't long to be out there.
Linda  


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, May 05, 2014 2:43 PM
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Basement Lab

Our main Histology lab at UW Madison Hospital is in the basement of the VA 
(They're) attached. We have to go UP a floor to get to the morgue. Although why 
they put the morgue on the main level is beyond me... Oh, wait. government 
administration... I am at one of the outlying clinics and at least we can walk 
by windows once in a while. :)  But for some reason we do get paid more than 
the med techs here. Knock me over with a feather. 
Claire


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RE: [Histonet] Xylene substitute for processing GI biopsies

[Blazek, Linda]

Lori,

We use Formula 83 from CBG for both processing and staining.  We have used this 
for years with no problem for our GI biopsies.  It wouldn't do any good to 
share my processing schedule with you though since we have a different 
processor.  If you want to talk or any further information feel free to contact 
me.  We also recycle our Formula 83.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies

Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl 
alcohol) and after sectioning you can use dishwasher soap before going directly 
to the staining procedure. After staining you need nothing but an oven to dry 
out the stained sections and go diractly to coverslipping.
If you want details on the above please go to: 
http://www.histosearch.com/rene.html and read my articles on the subject, 
including processing protocols.
If you follow the instructions, you will have a totally xylene free lab.
René J. 
On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt  wrote:
  
Hi there!
I have spent a lot of time reading the archives, but still need some specific 
and current input/advice. 
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with 
a xylene substitute.  I need insight on protocols, brands, effects on 
staining. The good, the bad, the ugly. I have only used xylene in my many 
years of experience!  This is completely new (to me), and rather overwhelming. 
It would be ideal to use the same substitute in the staining process, so any 
consideration with that is also appreciated. 
I understand this has been discussed, at length in the past, and I 
apologize I am just hoping to zero in on the best case scenario as a 
starting point.

Thanks, in advance, for your help!
Lori

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[Histonet] RE: Retirement

[Blazek, Linda]

Congratulations on your retirement!  May you enjoy your time and remember 
you're supposed to take a picture of the sunrise on your first day of 
retirement!  
And personally I don't think vendors are frowned upon.  They are full of wise 
info.  What's frowned upon is blatant advertising.  Even then the delete key is 
on the keyboard.  
Good Luck!

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of VanTilburg, Walt
Sent: Tuesday, April 15, 2014 3:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Retirement

I know it is frowned upon for vendors to write to Histonet, but I hope you will 
indulge me as I have no easy way to say good bye to some great people.  I am 
retiring next Tuesday the 22nd and for the past 11 year I have met many of you 
in histo land and have enjoyed the help you all have given me.  Without fail 
every time I entered a lab you unselfishly answered my questions and helped me 
understand the work flow in the AP labs.  For the first couple of years I had 
to write down and Google almost every third word you said, but I started to 
catch on and you input and our discussions helped to shape the products that we 
developed.  For that I am grateful.
I met a lot of people and made a lot a good friends and saying good bye is hard 
but the truth is you wore me out and I need to take a break.  I would list all 
of the folk who were especially helpful but the list would be really long so 
this is just a blanket thank you and good bye.

Walt
Walter Van Tilburg
Vice President of Business Development
General Data Healthcare, Inc.
26500 Bruce Road
Bay Village, Ohio 44140

440-823-5495 - Mobile
440-808-8983 - Office
440-808-8995 - Fax
wvantilb...@general-data.com
Visit our web site http://www.general-data.com/healthcare/

Great news! Triangle Biomedical Sciences (TBS) is now a division of General 
Data Healthcare!
Visit us online at www.general-data.com/hc  and www.trianglebiomedical.com.

http://www.general-data.com/news/general-data-acquires-triangle-biomedical-sciences



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RE: [Histonet] GI Biopsies

[Blazek, Linda]

3 levels - 1 slide

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
imhype...@aol.com
Sent: Thursday, March 13, 2014 2:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI Biopsies


Good afternoon all,
 I was just curious about how your institutions handle GI biopsies, 
specifically how many slides you cut off the bat.  We presently cut 2 levels on 
each GI biopsy block, but I'm hearing that more and more places only cut 1 
slide per GI biopsy block.  Please share what you are doing at your 
establishment.
Thank you 
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RE: [Histonet] Mailing Freebie

[Blazek, Linda]

You're supposed to stick it on your phone and put your money in it.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
joellewea...@hotmail.com
Sent: Friday, March 07, 2014 12:32 PM
To: Goins, Tresa; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Mailing Freebie

I thought earlier this week they sent out an explanatory email saying it was a 
phone holder? Perhaps  I  dreamt this, it has been a week. 

Sent from my Verizon Wireless 4G LTE Smartphone

- Reply message -
From: "Goins, Tresa" 
To: "histonet@lists.utsouthwestern.edu" 
Subject: [Histonet] Mailing Freebie
Date: Fri, Mar 7, 2014 12:03 pm




OK - I may be missing something, but what is that "blue adhesive" pocket that 
was sent out with histology professionals' day literature?
Just curious . . . . .

Happy Friday.

Tresa





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RE: [Histonet] Cost per test

[Blazek, Linda]

You might want to get ahold of Tim Morken also.  He has a great spreadsheet.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Thursday, March 06, 2014 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cost per test

Rene,

I know that you have discussed this on histonet several times and you have 
written a paper - can you send me a copy of your paper? We are having to do 
some accounting here to justify our charges and I think your paper would help.

Andrea




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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[Histonet] 88342 - again

[Blazek, Linda]

Good morning,

Has anyone seen or know anything yet as to how insurances are paying for 
multiple 88342's on a case for non-Medicare patients?  It seems as though no 
one yet is sure what is going on.
Thanks,
Linda

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[Histonet] RE: New GHS labels

[Blazek, Linda]

Here is what I have in my notes for the new standards.  I think you're probably 
safe with what you currently have as long as you've don't  the training that 
should have been done by Dec 1st 2013.

The first compliance date of the revised HCS is December 1, 2013. By that time 
employers must have trained their workers on the new label elements and the SDS 
format.

All hazardous chemicals shipped after June 1, 2015, must be labeled with 
specified elements including pictograms, signal words and hazard and 
precautionary statements. However, manufacturers, importers, and distributors 
may start using the new labeling system in the revised HCS before the June 1, 
2015 effective date if they so choose. Until the June 1, 2015 effective date, 
manufacturers, importers and distributors may maintain compliance with the 
requirements of HazCom 1994 or the revised standard. Distributors may continue 
to ship containers labeled by manufacturers or importers (but not by the 
distributor themselves) in compliance with the HazCom 1994 until December 1, 
2015.

Linda

Linda Blazek HT (ASCP)
GI Pathology of Dayton
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, February 27, 2014 2:34 PM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] New GHS labels 

I'm getting ready for my CAP inspection and I came along information about the 
new Global warning labels that were to replace the NFPA labels we currently use 
for our chemicals. Is everyone using these or can we still use the NFPA label 
and placards? I remember hearing about it at a seminar just shortly after out 
inspection in 2012 and never thought of it again. Thanks, Cheri

Cheryl Miller HT ASCP
Histology Supervisor
Hygiene Officer
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 493 0403
Fax 402 731 8653


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RE: [Histonet] Purple haze on H&E slides

[Blazek, Linda]

Haley,
 We use Leica's Selectech also and occasionally have the same issue.  I don't 
think your ETOH after the eosin is a problem.  If you add a 95% you will leach 
out the eosin a bit.  I think you should add another 15 - 30 seconds in the 
acid alcohol.  One of the things we find here though is that there can be a 
great variation to the pH of the water.  That affects the results of the stain. 
Specifically the "purple haze"  Go Jimi!

Purple haze all in my brain 
Lately things just don't seem the same 
Actin' funny, but I don't know why 
'Scuse me while I kiss the sky 

Linda
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Huggins, Haley 
- MRMC
Sent: Tuesday, February 11, 2014 12:07 PM
To: Campbell, Tasha M.
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Purple haze on H&E slides

We are using the Leica Selectech brand. No additives to the waterbath. We are 
using xtra or superfrost slides. The protocol is as follows:
3 min xylene
3 min xylene
1 min 100% ETOH
2 min 100% ETOH
1 min 95% ETOH
1 min water rinse
4 min hematoxylin
2 min water rinse
15 sec acid alcohol
1 min water rinse
1.5 min bluing
1 min water rinse
1 min 95% ETOH
5 min eosin
30 sec 100% ETOH
1 min 100% ETOH
1 min 100% ETOH
1 min xylene
2 min xylene

Haley

-Original Message-
From: Campbell, Tasha M. [mailto:tmcampb...@fmh.org] 
Sent: Monday, February 10, 2014 5:38 PM
To: Huggins, Haley - MRMC
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Purple haze on H&E slides

What brand of reagents are u using and what is your protocol? What slides r u 
using and r u using gelatin in ur waterbath?

Tasha

Sent from my iPhone

> On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" 
>  wrote:
> 
> I am trying to get the histology lab up and running and I am having 
> difficulties getting the H&E Stain just right. I am nearly there, but I keep 
> getting a purple bleed or haze around the tissue. Any suggestions on getting 
> rid of this? I actually got rid of it at one point, but the medical director 
> wasn't happy with the stain. I appreciate any suggestions from the histology 
> world.
> 
> Haley Huggins
> ___
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[Histonet] RE: IHC

[Blazek, Linda]

I second this!  Since I've not worked with animal tissue I didn't know quite 
how to respond to the question but in regards to flexibility, ease of use and 
unquestionably remarkable support I would not hesitate to check into the 
Intellipath FLX.   

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Thursday, January 23, 2014 3:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC

I've used many different platforms and have liked most.  Currently we are using 
BioCare. We LOVE our BioCare Intellipath FLX for our IHC platform.  It is very 
cost effective, completely open, and has very user friendly software. BioCare 
has been a super company to work with.
Fabulous customer support with a very knowledgeable technical staff.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 16
Date: Thu, 23 Jan 2014 11:49:15 -0500
From: Erin Sarricks 
Subject: [Histonet] IHC-start-up

Hi all-
Our histopathology lab is looking to
set up an IHC component to service the request of our clients.  All work is on 
animal tissue.  We will probably run about 1,000 IHC slides the first year and 
hope to increase the workload each year.  Some  stains we would run would 
include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and 
appeared to be a good fit for our needs.  Does anyone have any other advice on 
other machines we should look into?  Any information or advice would be greatly 
appreciated. Thank you!

Regards,
Erin Sarricks, HT (ASCP)

*
-



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RE: [Histonet] Histology and hip replacement

[Blazek, Linda]

That's what I was going to suggest.  "open a drawer and put a tray or board on 
it"  We do that any way as a place to set the slides and other paraphernalia 
that you may need.  It keeps from having to reach over or around.  
Linda

Linda Blazek HT (ASCP)
GI Pathology of Dayton
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Friday, January 10, 2014 11:57 AM
To: 'fredandlaura...@sbcglobal.net'; Sherwood, Margaret
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histology and hip replacement

I would suggest that if you have a table (or can open a drawer and put a tray 
or board on it), put your water bath beside you. Then you wouldn't need to 
"push off". Maybe that would be a solution?

Best wishes for a speedy and complete recovery! j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
fredandlaura...@sbcglobal.net
Sent: Friday, January 10, 2014 11:53 AM
To: Sherwood, Margaret
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histology and hip replacement

Thanks Peggy,

  I've heard many positive accounts and they help me maintain a positive 
attitude.  My main restrictions, for a few months are; no crossing of legs, no 
turning of feet or knees inward, and no bending at the hip past 90 degrees.  
Also, items need to be located between my legs before lifting.  One of my 
concerns is the ability to move between microtome and water bath, while 
sitting, without violating those restrictions.  I may need to get creative in 
the layout of the lab.
 On a positive note, the Leica RM2255 we purchased earlier in the year, 
helped alleviate my repetitive motion problems.  Thank you Histonetters for 
helping me with that selection.  I apologize for being so long winded.  I guess 
spending the days locked inside, interacting only with a couple snoring 
dachshunds, might be taking it's toll.

Fred

Sent from my iPad

> On Jan 9, 2014, at 3:36 PM, "Sherwood, Margaret"  
> wrote:
>
> I've had 2 hip replacements; facing having the cup replaced on the first one 
> (20 years since replaced).  I have had no issues, ergonomically or otherwise. 
>  It made a world of difference in the quality of my life.
>
> Good luck!
>
>
>
>
> Peggy Sherwood
> Research Specialist, Photopathology
> Wellman Center for Photomedicine (EDR 214) Massachusetts General 
> Hospital
> 50 Blossom Street
> Boston, MA 02114-2696
> 617-724-4839 (voice mail)
> 617-726-6983 (lab)
> 617-726-1206 (fax)
> msherw...@partners.org
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
> fredandlaura...@sbcglobal.net
> Sent: Thursday, January 09, 2014 2:51 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Histology and hip replacement
>
> Happy New Year out there in Histoland,
>
> Here in Dayton Ohio, we're coming out of the deep freezer back into the 
> refrigerator.  I just had hip replacement surgery.  Beats some ball dropping 
> on Times Square, right?  I'm a one person lab, and am looking for some input 
> on issues I'll be dealing with when I get back to work.  Ergonomics, etc.
>
> Thanks in advance,
> Fred
>
> Sent from my iPad
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>
>
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> information, please contact the sender and properly dispose of the e-mail.
>

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RE: [Histonet] Safety items

[Blazek, Linda]

If it isn't documented it isn't done!  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Tuesday, January 07, 2014 11:09 AM
To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Are they looking for documentation that it's been checked, or will they take 
your word for it?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert 
A
Sent: Tuesday, January 07, 2014 10:51 AM
To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Newest CAP inspection stupid thing. They want you to periodically check your 
spill kit for clumping, since it does not have an expiration date. I challenged 
it, but don't know if will go through. 

Robert A. Eytalis
Laboratory Manager
robert-eyta...@riversidehealthcare.net
Phone: (815) 935-7256 ext. 5186
(815) 935-7535
Fax (815) 935-7068

Riverside Medical Center
350 N. Wall Street - Kankakee, IL 60901


http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f
  |  
http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh 
[amurv...@advancederm.net]
Sent: Tuesday, January 07, 2014 9:25 AM
To: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Safety items

Don't forget to make sure you have a spill kit and its labeled well with easy 
access,  Inspectors will ask employees if they know where it and your fire 
extinguisher and alarm are located. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, January 07, 2014 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Safety items


What safety items do you keep in your lab besides the normal apron, gloves, eye 
wash stations and shower?  Thanks!

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Re: [Histonet] OT: RE: wax on the floors

[Blazek, Linda]

I'm sure there won't be much left when we're finished. 

Sent from my iPhone

On Dec 11, 2013, at 6:31 PM, "Ingles Claire"  wrote:

> When you're finished with him, send Bruce my way!!!
> Claire
> 
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Blazek, Linda 
> [lbla...@digestivespecialists.com]
> Sent: Wednesday, December 11, 2013 2:32 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] RE: wax on the floors
> 
> I'm still looking for Bruce the stripper!
> We use to use a paraffin floor scraper kind of like a scraper on the end of a 
> broom stick but somehow it disappeared.  We now use a paraffin scrapper that 
> is much easier on the floor.  The one designed for scrapping the floor had a 
> blade that had a tendency to gouge the floor.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
> Sent: Wednesday, December 11, 2013 3:17 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] Re: wax on the floors
> 
> Ok, I could not resist.  Let's see, I have used:
> Paint scraper
> Slide (when I first started)
> Spackle spatula taped to a broomstick (when I worked at small, poor place) 
> Ice thingy (removes the ice from car windshield)  hey I live in Houston, we 
> don't have ice Floor scraper from a carpet/vinyl floor company (when I worked 
> in a fancy lab)
> 
> Also, don't let housekeeping wax the floors.
> 
> 
> 
> Sincerely,
> 
> Toysha N. Mayer, MBA, HT(ASCP)
> tnma...@mdanderson.org
> Instructor/Education Coordinator
> Program in Histotechnology
> School of Health Professions
> MD Anderson Cancer Center
> 713-563.3481
> 
> 
> Message: 1
> Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST)
> From: Paula Pierce 
> Subject: Re: [Histonet] wax on the floors
> To: "Victor A. Tobias" ,Histonet
>
> Message-ID:
><1386784143.76836.yahoomail...@web5706.biz.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
> 
> A brand name! LOL
> ?
> Paula K. Pierce, HTL(ASCP)HT
> President
> Excalibur Pathology, Inc.
> 5830 N Blue Lake Dr. Please note new address!
> Norman, OK 73069
> 405-759-3953 Lab
> 405-759-7513 Fax
> www.excaliburpathology.com
> 
> 
> 
> From: Victor A. Tobias 
> To: Paula Pierce ; Nancy Schmitt 
> ; Histonet 
> Sent: Wednesday, December 11, 2013 11:25 AM
> Subject: RE: [Histonet] wax on the floors
> 
> 
> Is Bruce a Brand name or the employee?
> 
> Victor Tobias HT(ASCP)
> Clinical Applications Analyst
> Harborview Medical Center
> Dept of Pathology Room NJB 244
> Ninth & Jefferson
> Seattle, WA 98104
> vtob...@u.washington.edu
> 206-744-2735
> 206-744-8240 Fax
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
> Sent: Wednesday, December 11, 2013 9:17 AM
> To: Nancy Schmitt; Histonet
> Subject: Re: [Histonet] wax on the floors
> 
> Bruce floor stripper!
> ?
> Paula K. Pierce, HTL(ASCP)HT
> President
> Excalibur Pathology, Inc.
> 5830 N Blue Lake Dr. Please note new address!
> Norman, OK 73069
> 405-759-3953 Lab
> 405-759-7513 Fax
> www.excaliburpathology.com
> 
> 
> 
> From: Nancy Schmitt 
> To: "histonet@lists.utsouthwestern.edu" 
> Sent: Wednesday, December 11, 2013 11:05 AM
> Subject: [Histonet] wax on the floors
> 
> 
> Happy Hump Day!
> 
> What is everyone doing to clean floors of paraffin?? Does anyone have any 
> great secrets to how the floors are cleaned of the debris?
> 
> Thanks for any input!
> 
> Nancy
> 
> Nancy Schmitt HT, MLT(ASCP)
> Histology Coordinator
> United Clinical Laboratories
> 
> 
> 
> 
> NOTICE: This email may contain legally privileged information. The information
> is for the use of only the intended recipient(s) even if addressed
> incorrectly. If you are not the intended recipient, please notify the sender
> that you have received it in error and then delete it along with any
> attachments. Thank you.
> 
> 
> 
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[Histonet] RE: wax on the floors

[Blazek, Linda]

I'm still looking for Bruce the stripper! 
We use to use a paraffin floor scraper kind of like a scraper on the end of a 
broom stick but somehow it disappeared.  We now use a paraffin scrapper that is 
much easier on the floor.  The one designed for scrapping the floor had a blade 
that had a tendency to gouge the floor.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Wednesday, December 11, 2013 3:17 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: wax on the floors 

Ok, I could not resist.  Let's see, I have used:
Paint scraper
Slide (when I first started)
Spackle spatula taped to a broomstick (when I worked at small, poor place) Ice 
thingy (removes the ice from car windshield)  hey I live in Houston, we don't 
have ice Floor scraper from a carpet/vinyl floor company (when I worked in a 
fancy lab) 

Also, don't let housekeeping wax the floors.



Sincerely,

Toysha N. Mayer, MBA, HT(ASCP)
tnma...@mdanderson.org
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
713-563.3481


Message: 1
Date: Wed, 11 Dec 2013 09:49:03 -0800 (PST)
From: Paula Pierce 
Subject: Re: [Histonet] wax on the floors
To: "Victor A. Tobias" ,Histonet

Message-ID:
<1386784143.76836.yahoomail...@web5706.biz.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

A brand name! LOL
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



 From: Victor A. Tobias 
To: Paula Pierce ; Nancy Schmitt 
; Histonet 
Sent: Wednesday, December 11, 2013 11:25 AM
Subject: RE: [Histonet] wax on the floors
 

Is Bruce a Brand name or the employee?

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB 244
Ninth & Jefferson
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Wednesday, December 11, 2013 9:17 AM
To: Nancy Schmitt; Histonet
Subject: Re: [Histonet] wax on the floors

Bruce floor stripper!
?
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Dr. Please note new address!
Norman, OK 73069
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com



From: Nancy Schmitt 
To: "histonet@lists.utsouthwestern.edu" 
Sent: Wednesday, December 11, 2013 11:05 AM
Subject: [Histonet] wax on the floors


Happy Hump Day!

What is everyone doing to clean floors of paraffin?? Does anyone have any great 
secrets to how the floors are cleaned of the debris?

Thanks for any input!

Nancy

Nancy Schmitt HT, MLT(ASCP)
Histology Coordinator
United Clinical Laboratories




NOTICE: This email may contain legally privileged information. The information
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incorrectly. If you are not the intended recipient, please notify the sender
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End of Histonet Digest, Vol 121, Issue 15
*

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[Histonet] RE: 2014 IHC CPT coding

[Blazek, Linda]

88342 for the first IHC antibody then 88343 for subsequent antibodies on the 
SAME SLIDE is my understanding.  
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Tuesday, December 10, 2013 4:34 PM
To: Histonet
Subject: [Histonet] 2014 IHC CPT coding

My understanding is that 88342 is used for the first IHC antibody, then 88343 
is used for each subsequent antibody (per specimen part).  Is that correct?  
Thank you.



Richard



Richard W. Cartun, MS, PhD

Director, Histology & Immunopathology

Director, Biospecimen Collection Programs

Assistant Director, Anatomic Pathology

Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 545-1596 Office

(860) 545-2204 Fax

richard.car...@hhchealth.org

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RE: [Histonet] Yahoo link

[Blazek, Linda]

Wow!  And I've survived 40 years with only 2 "nicks" in this perilous field!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Tuesday, December 03, 2013 11:41 AM
To: Mike Tighe
Cc: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Yahoo link

Thank you for sharing that!  Yikes!

Sent from my iPhone

On Dec 3, 2013, at 6:31 AM, Mike Tighe  wrote:

> Anybody wonder who has the most harzardous job to your health? We're Number 
> One!!!
> 
> 
> 
> http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html
> 
> 
> 
> Mike
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[Histonet] job opening Dayton Oh area

[Blazek, Linda]

Come join our team!  We have an opening for a full time tech to perform all  
routine tissue processing embedding, cutting and staining, perform a minimum 
number of special stains and re-cuts, perform immuno stains on an automated 
immuno stainer, also perform histology lab maintenance to include maintenance 
of instrumentation, assist in compliance with CLIA regulations, providing 
completed preventative maintenance and temperature log sheets for Histology 
lab. Email me for additional information or send your resume if you're 
interested in joining our great team!

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

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RE: [Histonet] India Ink for inking surgical margin borders

[Blazek, Linda]

LOL  It's Friday...  I can't resist!  You SUED India Ink?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
wanda.sm...@hcahealthcare.com
Sent: Friday, October 11, 2013 11:38 AM
To: ecre...@cmblabs.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] India Ink for inking surgical margin borders

We sued India Ink from an art supply store for years.

WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

This email and any files transmitted with it may contain PRIVILEGED or 
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this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ed Crespo
Sent: Thursday, October 10, 2013 12:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] India Ink for inking surgical margin borders

I normally purchase India Ink from one of our vendors, but know it's also sold 
and used at artist supply shops.  Does anyone know if I can used the artist 
india ink for Pathology use?  Really, the only issue would be if the ink stays 
on the tissue during processing right? Please advise.

Ed Crespo, CT(ASCP)
Anatomic Pathology Manager
Safety Officer


10700 Walker Street
Cypress, CA 90630
phone: 714 880.3330
fax: 714 816.1511
email: ecre...@cmblabs.com
cmblabs.com
 
 
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attachments from your computer and any archival/backup copies.  Thank you.
 



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[Histonet] RE: Pathology charges

[Blazek, Linda]

I know you have to have the correct ICD-9 codes attached but have not heard of 
pre-authorization.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch
Sent: Wednesday, October 09, 2013 12:13 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Pathology charges

We've been told that before our pathologists can order/run any of these stains 
(CPT 88313, 88342 or 88360) on our pathology specimens, we must obtain a 
pre-authorization from the insurance carrier or the payment will be denied.
Has anyone else run into this?  How are you efficiently handling that?

Maray Weirauch
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RE: [Histonet] Lab Chairs

[Blazek, Linda]

Mercedes Medical has something like that too.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Tuesday, October 01, 2013 10:06 AM
To: Rathborne, Toni; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Lab Chairs

I cannot find the company I originally order my saddle chairs from but the link 
below has a picture that looks a lot like what I have.  The chairs I have are 
from  CNS Chairs 800 Boylston St Suite 1600 Boston, MA  1-800-457-3120.  Sorry 
it took a while to get the information I had to go back through my orders to 
find it.

http://www.sitbetter.com/view/chair/ost-st205/backless-tall-stool-with-saddle-seat-and-seat-angle/?utm_campaign=shoppingengine&utm_medium=shoppingengine&utm_source=shopzilla&zmam=91792832&zmas=1&zmac=11&zmap=OST-ST205

Roberta Horner HT/HTL
Penn State University
Animal Diagnostic Lab

-Original Message-
From: Rathborne, Toni [mailto:trathbo...@somerset-healthcare.com]
Sent: Friday, September 27, 2013 7:36 AM
To: Roberta Horner; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Lab Chairs

Do you have a link to where we could see them? 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Friday, September 27, 2013 7:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Lab Chairs

I have 2 saddle chairs and I really like them.  You have to sit straight on 
them, no slouching.  I have less back aches since I got them.
Roberta Horner HT/HTL
Penn State University
Animal Diagnostic Lab
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RE: [Histonet] The Good Old Days...

[Blazek, Linda]

One has been missed!  Doing gross without gloves.  There was a pathologist that 
claimed to be able to feel the lymph nodes much better without gloves.  

-Original Message-
From: James Watson [mailto:jwat...@gnf.org] 
Sent: Friday, September 20, 2013 3:20 PM
To: 'Goins, Tresa'; Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, 
Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

OK

1.  Making formalin and Grossing with an old metal table fan to blow the 
formalin fumes away.  
2.  Finding the technicon hung up in the morning with the samples dried out.
3.  Figuring out how to reprocess the tissue
4.  Pan embedding and having the paper labels float away from the samples 
in the pan.
a.  Bunsen burner needed
5.  Big kitchen knife heated over a Bunsen burner to make blocks
6.  Soaking re-useable metal cassettes in a bucket of xylene.  (next to 
where you were pan embedding)
7.  Sharpening a knife on multiple stones then stropping on leather then 
cloth.
8.  Over tightening the microtome clamp on the paraffin block and having to 
re-embed because the block broke.
9.  Making staining solutions (some of us still do this).
a.  Making Schiffs regent without a hood
b.  Cresyl violet stain powder floating around the room,  watch 
where you put your hands
c.  Silver nitrate spilled on the counter where you just put your 
hand then wiped your face. 
10. Staining slides in open staining dishes with no fume hood.
11. Wiping the whole room down with xylene to clean the wax up.
12. Autopsies in the afternoon,
13. Whole human brains soaking in the sink in 5 gallons of 20% formalin 
overnight.

Oh boy,  really miss them good old days.  Life was easier then!

Hey Teri,   When are we going to get our new coverslipper in?  We were 
spoiled by the demo unit.

Jamie


James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation Tel    858-332-4647 
Fax   858-812-1915 jwat...@gnf.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Friday, September 20, 2013 11:33 AM
To: Blazek, Linda; Victor A. Tobias; Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

I've stropped blades until they looked like scythes - and I got pretty good at 
adjusting those three screws to line up my block on the microtome.



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, September 20, 2013 12:15 PM
To: Victor A. Tobias; Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

How many can strop a knife?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias
Sent: Friday, September 20, 2013 2:01 PM
To: Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

Seems like no one has touched upon the fine art of knife sharpening. Sometimes 
I felt like an executioner sharpening my axe to the point of splitting a hair. 
Heaven forbid if you hit a staple. That part of the blade was no useless for 
days to weeks until the daily sharpening ground it out. Life is pretty good 
when you just pull out another blade from the plastic box.

Victor 

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB 244
Ninth & Jefferson
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
Privileged, confidential or patient identifiable information may be contained 
in this message. This information is meant only for the use of the intended 
recipients. If you are not the intended recipient, or if the message has been 
addressed to you in error, do not read, disclose, reproduce, distribute, 
disseminate or otherwise use this transmission. Instead, please notify the 
sender by reply e-mail, and then destroy all copies of the message and any 
attachments.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, September 20, 2013 10:40 AM
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Good Old Days...

Hear hear!  I agree and was just saying I love hearing the stories (although I 
am not young).  Thanks all for sharing these memories and lessons!

Sent from my iPhone

On Sep 20, 2013, at 10:13 AM, "Davis, Cassie"  wrote:

> I enjoy hearing sincere reminiscing...Even though us "kids" don'

RE: [Histonet] The Good Old Days...

[Blazek, Linda]

How many can strop a knife?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias
Sent: Friday, September 20, 2013 2:01 PM
To: Cristi Rigazio; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] The Good Old Days...

Seems like no one has touched upon the fine art of knife sharpening. Sometimes 
I felt like an executioner sharpening my axe to the point of splitting a hair. 
Heaven forbid if you hit a staple. That part of the blade was no useless for 
days to weeks until the daily sharpening ground it out. Life is pretty good 
when you just pull out another blade from the plastic box.

Victor 

Victor Tobias HT(ASCP)
Clinical Applications Analyst
Harborview Medical Center
Dept of Pathology Room NJB 244
Ninth & Jefferson
Seattle, WA 98104
vtob...@u.washington.edu
206-744-2735
206-744-8240 Fax
=
Privileged, confidential or patient identifiable information may be contained 
in this message. This information is meant only for the use of the intended 
recipients. If you are not the intended recipient, or if the message has been 
addressed to you in error, do not read, disclose, reproduce, distribute, 
disseminate or otherwise use this transmission. Instead, please notify the 
sender by reply e-mail, and then destroy all copies of the message and any 
attachments.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, September 20, 2013 10:40 AM
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] The Good Old Days...

Hear hear!  I agree and was just saying I love hearing the stories (although I 
am not young).  Thanks all for sharing these memories and lessons!

Sent from my iPhone

On Sep 20, 2013, at 10:13 AM, "Davis, Cassie"  wrote:

> I enjoy hearing sincere reminiscing...Even though us "kids" don't know how 
> good we have it, some of us enjoy having an "old tech" beside us on the 
> bench. I find weeding through the sarcasm can be profitable and in doing so 
> have learned so much. What the old techs did on a daily basis, we only did in 
> the practice lab and when the automated instruments and pre-made solutions 
> that we have come to rely on fail, experience is so very valuable. Only by 
> their blood, sweat and tears have we benefitted however, we have so far to 
> go, let's do it together.
> 
> Cassandra Davis
> cda...@che-east.org
> 302-575-8095
> 
> 
> 
> 
> 
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Catholic 
> Health East and is intended for the sole use of the intended 
> recipient(s).
> It may contain information that is privileged and confidential.  Any 
> unauthorized review, use, disclosure, or distribution is prohibited. 
> If you are not the intended recipient, please delete this message, and 
> reply to the sender regarding the error in a separate email.
> 
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RE: [Histonet] Re: bunsen burner at the embedding center

[Blazek, Linda]

Lots of us and we survived!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Tournear
Sent: Friday, September 20, 2013 1:55 PM
To: Paula Sicurello
Cc: Histonet@lists.utsouthwestern.edu; Bob Richmond
Subject: Re: [Histonet] Re: bunsen burner at the embedding center

How many of us smoked and drank coffee at our microtomes? Or while doing 
autopsies? Those were the days and a lot of us are still around. Lol

Sent from the iPhone of Kim Tournear 🇺🇸 😃

On Sep 20, 2013, at 8:44 AM, Paula Sicurello  wrote:

> I worked for the Navy Hospital in San Diego, way back in the day, and 
> we did an extraction with fuming HCl (straight, 100% HCl for folks who 
> didn't know what fuming meant).  Several people would get nosebleeds 
> every time they performed the extraction.  I finally got one of those 
> fans that looks like an airplane propellor in a cage and pried open 
> the painted shut windows.  I dared anyone to stop me, no one did!  1 
> was quite gutsy for a
> 22 year old.
> The Petty Officers had me make 10N NaOH and forgot to tell me how hot 
> it got.  I almost dropped it when I picked it up.
> 
> Plus I had to swim to work, both ways in a rip tide-battleing sharks 
> and jelly fish, while sloshing around in an earthquake!  It is San 
> Diego after all.
> 
> Happy Friday!
> 
> Paula
> --
> Paula Sicurello, HTL (ASCP)
> Supervisor, Clinical Electron Microscopy Laboratory Duke University 
> Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 
> 27710
> P: 919.684.2091
> HIPAA Privacy Notification: This message and any accompanying 
> documents are covered by the Electronic Communications Privacy Act, 18 
> U.S.C. 2510-2521, and contain information intended for the specific 
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> the intended recipient, you are hereby notified that you have received 
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> the taking of any action based on the contents of this information is 
> strictly prohibited . If you have received this communication in 
> error, please notify us immediately by e-mail, and delete the original 
> message.
> 
> 
> On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen  wrote:
> 
>> yet here we are..how much of what they tell us is hyperbole?
>> 
>>> Date: Fri, 20 Sep 2013 14:50:24 +
>>> From: mucra...@comcast.net
>>> To: rsrichm...@gmail.com
>>> Subject: Re: [Histonet] Re: bunsen burner at the embedding center
>>> CC: histonet@lists.utsouthwestern.edu
>>> 
>>> 
>>> 
>>> I actually knew the person you are speaking of and it was his 
>>> favorite
>> trick.  We should be careful these younger people are using gloves 
>> for everything now to protect themselves.  W hen I started in 
>> Histology even the pathologists cleaned the paraffin off their hands 
>> with xylene and encouraged everyone to do the same .  We used so many 
>> things that are now not even allowed to be open on this planet and 
>> did know how dangerous or serious the possible issues could be over time.
>>> 
>>> 
>>> Pam Marcum
>>> 
>>> 
>>> 
>>> - Original Message -
>>> From: "Bob Richmond" 
>>> To: "Histonet@lists.utsouthwestern.edu" <
>> histonet@lists.utsouthwestern.edu>
>>> Sent: Friday, September 20, 2013 9:35:55 AM
>>> Subject: [Histonet] Re: bunsen burner at the embedding center
>>> 
>>> I distinctly remember when I was a resident at Johns Hopkins in the 
>>> late 1960s that the histotechs would smoke while staining and 
>>> coverslipping without much ventilation. When I suggested to the 
>>> chief technologist (who later died of smoking related disease at 65, 
>>> but at least he hadn't set himself afire) that this wasn't such a 
>>> good idea, he responded by
>> stubbing
>>> out a lighted cigarette in a Stender dish full of xylene (apparently 
>>> you can do this trick with gasoline also, but don't try it at home please).
>>> 
>>> Buffering formalin was prohibited back then, and they removed the
>> formalin
>>> pigment by passing the sections through a concentrated solution (20 
>>> or
>> 30%)
>>> of picric acid in acetone. I'm glad he didn't try the cigarette 
>>> trick in THAT Stender dish.
>>> 
>>> Fast-forward nearly half a century, and in the three labs I'm 
>>> working in I'm still grossing formalin-fixed tissue with minimal 
>>> ventilation, but at least people aren't allowed to smoke in the lab any 
>>> more.
>>> 
>>> Bob Richmond
>>> Samurai Pathologist
>>> Maryville TN
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>> __

RE: [Histonet] RE: bunsen burner at the embedding center

[Blazek, Linda]

Embedding center?  We had to pour melted paraffin into metal grid molds.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, September 20, 2013 10:09 AM
To: Weems, Joyce K.; 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center

 and it was always snowing and I had to walk 19 miles to work 
uphillboth ways. . We had to stock our cryostat with fresh cut blocks of 
ice from the river 10 miles away. (uphill, both ways) We had to start our 
Bunsen burner with steel and flint and we used our fingers instead of forceps.  
G. Gorden Liddy was a wimp compared to us 

Kids today don't know how good they got it! (They also don't know who G. Gordon 
Liddy was but can instinctively Google it) 

- Just some Friday fun for what has turned into a fun thread. - Oh wait, I 
forgot, this forum is for professional questions - so- Can you please 
unsubscribe me?

Have a great weekend! - Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Friday, September 20, 2013 8:48 AM
To: 'Edwards, Richard E.'; 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center

With no ventilation

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, 
Richard E.
Sent: Friday, September 20, 2013 9:46 AM
To: 'Jim Burchette'; Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: bunsen burner at the embedding center

And sit around smoking as  we  mounted the  sections from  xylene, in an open 
lab

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jim Burchette
Sent: 20 September 2013 14:41
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: bunsen burner at the embedding center

Back in the 70's we would boil metal base molds in water using a bunsen burner 
and a 3 legged ring stand.
On Sep 20, 2013 9:37 AM, "Davis, Cassie"  wrote:

> Hi Valerie,
> When I started in Histo in 90' everybody used the alcohol 
> burners...Open flame concern became a concerned and the separate 
> forcep warmers were purchase because the old embedding centers did not 
> have the nice warmers like the new ones do. The last place I worked at 
> had an old embedding center when I started but we weren't allowed open flames.
> Fortunately, we found an unused Bacteria Incinerator that Micro.
> wasn't using and used that until that embedding center died. That worked 
> great!
>
> Cassandra Davis
> cda...@che-east.org
> 302-575-8095
>
>
> From: "Hannen, Valerie" 
> To: "Histonet Post (histonet@lists.utsouthwestern.edu)" < 
> histonet@lists.utsouthwestern.edu>
> Sent: Thursday, September 19, 2013 11:04 AM
> Subject: [Histonet] Bunsen Burner
>
>
> Hi all..
>
> We are having a discussion/ disagreement in our department as far as 
> whether using a bunsen burner at the embedding center is against fire codes.
>
> What is the consensus??
>
>
> Thanks,
>
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief 
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.han...@parrishmed.com
>
> Confidentiality Notice:
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> If you are not the intended recipient, please delete this message, and 
> reply to the sender regarding the error in a separate email.
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RE: [Histonet] Unregistered HT

[Blazek, Linda]

Wow René, I didn't realize you had such a demeaning and disrespected career.  I 
have been in this profession for almost 40 years.  I have worked for some of 
the greatest pathologists you could ask for.  They have respected me, asked my 
opinions and shared a great deal of time at the microscope with me.  Maybe it 
has to do with attitude..

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, September 11, 2013 1:30 PM
To: Clare Thornton; 'Emily Sours'
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unregistered HT

The issue remains the WE cannot impose on others how we are viewed. No matter 
how many brain storming meetings there are about "demanding" respect the issue 
remains that those who have to respect us have to do so.
Short of a national strike (absolutely impossible to achieve) or a national 
union (that will be fought to the death) the only thing we can do is to stand 
for our rights of being treated as professionals every time somebody, either 
pathologist or administrator, disrespects us. And who, may I ask, will be able 
to do that in an economic situation as the present one? Who is going to risk 
his or her job just "to demand respect"?
That is an illusion. Unfortunately the economic situation impedes any action 
and those who can will keep disrespecting us as long as they can get away with 
it.
Having a good "position statement" about "what does it mean to be a 
professional" is an exercise in futility that probably for those who wrote it 
may have meant being able to have 1 or 2 days paid without actual work to do.
René J.



From: Clare Thornton 
To: Clare Thornton ; 'Emily Sours' 
 
Cc: "histonet@lists.utsouthwestern.edu"  
Sent: Wednesday, September 11, 2013 1:20 PM
Subject: RE: [Histonet] Unregistered HT


My apologies, the editorial was in the September 2011 issue of Journal of 
Histotechnology.  I have a pdf of it, will be happy to email.


Clare J. Thornton, HTL(ASCP)QIHC 
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton
Sent: Wednesday, September 11, 2013 10:20 AM
To: 'Emily Sours'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Unregistered HT

Look for a position paper presented by members of the NSH BOD in the Journal of 
Histotechnology after the national convention in Vancouver last year.  I'm not 
sure of the exact issue (thought I still had it on my desk).  This will explain 
what being a laboratory professional is and why it is so important that as 
histotechnologists we are viewed as such.


Clare J. Thornton, HTL(ASCP)QIHC 
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Wednesday, September 11, 2013 9:58 AM
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Unregistered HT

Do you get paid more with that title? Just curious, I'm not sure what 
"laboratory professional" means, as I'm not in that kind of lab.
Or is it a respect thing?

Emily

"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Tue, Sep 10, 2013 at 7:32 PM, Jennifer MacDonald wrote:

> As long as we do not need certification, licensure and minium 
> education requirements we will not be recognized as Laboratory Professionals.
>
>
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[Histonet] RE: Labels for outside blocks

[Blazek, Linda]

I do the same thing.  Adding a couple of drops of paraffin over the label has 
worked for us for years.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Thursday, August 29, 2013 10:43 AM
To: Morken, Timothy; Histonet
Subject: [Histonet] RE: Labels for outside blocks

We get the label with the block and stick it on ourselves with melted paraffin.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Wednesday, August 28, 2013 4:41 PM
To: Histonet
Subject: [Histonet] Labels for outside blocks

Has anyone found a very sticky label for labeling outside consult blocks,  ie 
that won't fall off?

Tim Morken
UCSF Pathology

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RE: [Histonet] (no subject)

[Blazek, Linda]

Hi,
I understand using lean at the embedding center but when it comes to the little 
things like the types of forceps it may be best to let the techs use what works 
best for them.  Everyone's hands are different and the feel of the forceps in 
their hands and dexterity come into play here.  If you have many different 
embedders maybe it would be best if each had and were responsible for their own 
forceps.  I don't know how that would counter affect the lean process.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanjeet 
Dhirubhai
Sent: Wednesday, August 07, 2013 9:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)



 Hi,

I am trying lean up at the embedding system. We have issues where staff have 
their own preference in regards to working on a specific forceps. I am trying 
to standardize this process and eliminate the hassle of having different types 
of forceps. Can anyone help me. Thanks Regards,
 
Sanjeet Dhirubhai - Supervisor Histology  MLT 
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[Histonet] RE: Accessioning specimes

[Blazek, Linda]

We label both the paperwork and the container with the surgical number.  We get 
Avery labels that are four across and have a program set up to print batches of 
consecutive numbers four across.  

We keep our embedding logs in the computer.

Linda Blazek HT (ASCP)
Manager/Supervisor
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Monday, July 08, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Accessioning specimes


When accessioning, do you label the paperwork AND the container with the 
surgical number?  If so, do you hand write the number or use a label maker?

Also, do you keep your embedding logs for 2 years like CAP requires QA and QC 
records?

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RE: [Histonet] Block labeling

[Blazek, Linda]

Put a label on the back of the block and then a couple of drops of paraffin.  
It acts like glue!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie
Sent: Monday, June 24, 2013 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Block labeling

Hi Everyone,

What is the best way to label blocks that are already embedded?  We have a 
large control bank that we are trying to relabel and make search able, however, 
most marking pens don't write well on paraffin covered blocks, even when we 
scrape them off.  Pencils work, not always the most legibly, so I was wondering 
if there is a clever solution that someone might have found.  We've tried 
putting stickers or labels on the back, but that falls right off.

Thanks in advance for any suggestions.

-- 

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869
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RE: [Histonet] Blade Rationing

[Blazek, Linda]

I don't think it's a matter of being reasonable or not, though I think 26 
blocks is a bit high.  
The major issue is taking away the discretion of the tech to make a decision as 
to the quality of the slide they are producing.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Benjamin
Sent: Tuesday, June 18, 2013 11:43 AM
To: Teresa Moore
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Blade Rationing

200 blocks per day / 3 techs = 66 blocks per day per tech 

66 blocks X 20 workdays a month = 1320 blocks a month

1320 blocks / 50 pack of blades = 26 blocks per blade

Resonable or not? You decide. 



Sent from my iPhone

On Jun 17, 2013, at 5:10 PM, Teresa Moore  wrote:

> I work in a hospital, there are three of us on this particular shift 
> and we cut approx. 200 blocks, give or take a few.  Our histo lab 
> manager is telling us we should only be using one pack of blades (50 
> per pack) a month.  I'm wondering what other techs think of this 
> especially lab managers and supervisors.
> 
> tmoor...@gmail.com
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Re: [Histonet] Blade Rationing

[Blazek, Linda]

>From a managers point of view, whoin my opinion that is a poor way to try to 
>cut expenses. It will only lead to recuts and possible loss of important 
>tissue. For the techs to understand the necessity to conserve is important but 
>the tech needs to use their discretion as to when a blade needs changing.  

Sent from my iPhone

On Jun 17, 2013, at 5:08 PM, "Teresa Moore"  wrote:

> I work in a hospital, there are three of us on this particular shift and we
> cut approx. 200 blocks, give or take a few.  Our histo lab manager is
> telling us we should only be using one pack of blades (50 per pack) a
> month.  I'm wondering what other techs think of this especially lab
> managers and supervisors.
> 
> tmoor...@gmail.com
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RE: [Histonet] Xylene substitutes for tissue processor clean cycle

[Blazek, Linda]

Carla, 
We use Formula 83 in the clean cycle.  We do change it more often than you 
would xylene though.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 11:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle

Hello all,

The technician who does preventive maintenance on our LX-120 tissue processor 
recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would 
appreciate any recommendations.

Thanks very much,


Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] Formula 83: xylene substitute

[Blazek, Linda]

Kiran,
We use Formula 83 on both staining and processing without any problem at all.  
You have to watch and either rotate or change your last Formula 83s on the 
stain line because you can't tell if there is water in it like you can with 
xylene.  We also use CBG's recycler to recycle Formula 83 without any problem.  
We have not had any problems with our IHC using Formula 83 either.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran
Sent: Tuesday, May 14, 2013 9:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formula 83: xylene substitute

Hello Everyone,

I would like to get some feedback from Formula 83 users. Are you using it only 
on the stainers or for tissue processing as well. What kind of validation 
process is required to switch to this substitute for ihc stains? We are 
interested to use it for both histo and cyto lab.
Any input is much appreciated.

Thank you in advance,

Kiran
Sent from my iPad
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RE: [Histonet] HIER pressure cookers....

[Blazek, Linda]

I second that!

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett 
M
Sent: Tuesday, April 30, 2013 9:54 AM
To: k. leigh adams; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HIER pressure cookers

Biocare Decloaker here !!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams
Sent: Tuesday, April 30, 2013 9:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HIER pressure cookers

Any input as to preferred instruments would be greatly appreciated...

Leigh
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[Histonet] RE: Primera slide printer

[Blazek, Linda]

Bea,
You can use need to use the clipped corner slides.  The best ones are the Epic 
Scientific slides available from CWS, Inc or the clipped corner Tanner slides 
available from Mercedes Medical.  I'm sure others carry them also.  These are 
the only two slides I've found that work really well in the printer.  I am very 
happy with my slide printer. Set up was very simple and interfacing with our 
LIS was very easy.  
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea 
DeBrosse-Serra
Sent: Tuesday, April 16, 2013 3:56 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Primera slide printer

Hello histonetters,

What kind of slides, from which vendor, do you use with the Primera slide 
printer? Do they need to be clipped corners? Are you happy with the slide 
printer? Does it have major issues?

Any information would be greatly appreciated.

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



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RE: [Histonet] slide printer

[Blazek, Linda]

Jennifer,

I have the Primera Signature Slide printer made by Primera Technology and sold 
by Creative Waste Solutions.  http://cwsincorp.com/
I really like it.  You can use it as a stand-alone or connected to your APLIS.  
It's compact and works very well.  You can print your slides with a color band 
across the top to distinguish different stains or types of tissue if you want 
or add bar coding.  I don't use bar coding yet so I can't say how well that 
function works.  The printing on the slides is very good.  There hasn't been 
any smudging or fading. There is a little bit of babysitting but not much.  
Tech support is fast and reliable when needed.  
If I can answer any other questions feel free to get in touch with me.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Monday, April 01, 2013 5:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slide printer

Surgipath used to have a small slide printer.  It would print individual 
slides.  Does anyone know if there is still such a printer available?
Thanks,
Jennifer
Mt. San Antonio College
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[Histonet] RE: Biocare Decloaker Nx Gen

[Blazek, Linda]

We have used the Decloaking Chamber for several years now and have had 
excellent results. The nicest thing is that there is consistency and 
reliability.  Each run will be the same as the one previous and having the 
ability to use the USB stick for documentation of time, pressure and 
temperature makes record keeping really easy.  We have had no complaints.  The 
only con I can come up with is that it comes in a big box that is hard to store 
if you have to send it back for some reason.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, 
Steven J
Sent: Thursday, March 28, 2013 1:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare Decloaker Nx Gen

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope 
unmasking (heat retrieval)? Also does anyone have any personal pros/cons with 
this instrument? We are thinking about getting one because of inconsistencies 
with our current method using a vegetable steamer. We use many different tissue 
types both animal and human. Also we use many different antibodies. Currently 
with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA 
combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 
2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure 
it wasn't due to fixation vimentin staining was performed and shows even 
staining. It's just certain antibodies that are giving us trouble even from 
multiple different companies. Any advice would be greatly appreciated and very 
beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartw...@cshs.org

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[Histonet] RE: Tracking OR specimens

[Blazek, Linda]

Michelle,
We have a check sheet that has all cases scheduled for the day.  There is a 
place to log how many specimens were collected for each case.  If there were no 
specimens collected then that is logged also.
Linda

Linda Blazek HT (ASCP)
lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle 
Lamphere
Sent: Monday, March 18, 2013 9:14 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Tracking OR specimens

Are there any histology labs that actively participate in auditing the 
Operating Room on a daily basis to make sure that histology receives all of the 
specimens that the OR should have submitted?  If so, how do you do this?  Or 
should the OR be solely responsible for making sure that they specimens make it 
to histology?

Michelle M Lamphere, HT (ASCP)
Senior Tech, Histology
Children's Medical Center
1935 Medical District Drive
Dallas, TX  75235
Office :214-456-2798
Histology: 214-456-2318
Fax:  214-456-0779


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[Histonet] RE: DAB source needed

[Blazek, Linda]

I have retired from shoveling but try Bio Care for your DAB.  They have a very 
nice one.  Also if you find someone to shovel send them my way!  Although 
Wisconsin to Ohio might be tricky.  But thanks for sending the snow our way.  :)
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty
Sent: Wednesday, March 06, 2013 10:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAB source needed

Hello All,

Can anyone recommend a DAB kit that is reliable and reasonable priced? Also, 
can someone shovel my driveway?

 

Thanks!

Sandy

 

Sandra Cheasty

Histology & Necropsy Supervisor

UW-Madison, Wisconsin 

 

 

 

 

 



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[Histonet] RE: Type I Water

[Blazek, Linda]

I have used tap water without any problems.  The only thing is you need to be 
sure you clean your waterbath well at the end of the day and dry it out.  What 
I experienced was hard water residue on the waterbath.
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Friday, February 15, 2013 9:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Type I Water

Clearly I sent this too quickly (early) - this is in regards to the water in 
our tissue float baths - does it HAVE to be Type I?
Thanks
Nancy

Good Morning-
I feel like I am racking up frequent flier miles on Histonet lately:) We are 
seeing bacteria in our type I.  This requires us to obtain our Type I from 
another of our lab sites.  Do I have any other options?  Like tap water?
Nancy Schmitt HT, MLT(ASCP)




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RE: [Histonet] Xylene Substitutes

[Blazek, Linda]

We have used Formula 83 from CBG for years with excellent results.  We use it 
for processing and staining.  We also recycle it making the cost very low.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, 
Michael J
Sent: Wednesday, February 06, 2013 11:47 AM
To: Adrienne Anderson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene Substitutes

We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with 
excellent results as well as cost savings.

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital 
| An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1526 
 

-Original Message-
From: Adrienne Anderson [mailto:rennie1...@yahoo.com]
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes

Hello all,

My lab is looking into xylene substitutes, and I'd love some feedback on what 
other labs are using. We currently use SubX, but are there other items out 
there more economical?

Thanks,
Adrienne
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RE: [Histonet] Joe Nocito's request -- oh my!

[Blazek, Linda]

I figured it was a test to see who would go on a rant!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Tuesday, February 05, 2013 2:42 PM
To: Cheryl
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Joe Nocito's request -- oh my!

Am I the only one who thinks its funny Joe the Toe did an unsubscribe e-mail 
and Cheryl Kerry copied an entire digest to reply.  Oh histonet pet peeves... 
haha

On Mon, Feb 4, 2013 at 1:05 PM, Cheryl  wrote:

> Oh no, Joe!  Say it ain't so!!  Please tell us this is just to take 
> off your military address and you aren't leaving us forever...
>
> Cheryl
>
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab by helping one GREAT Tech at a time.
> 281.852.9457 Office
> 800.756.3309 Phone & Fax
> ad...@fullstaff.org
>
> Sign up for the FREE newsletter AP News--updates, tricks of the trade 
> and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe'
> request to apn...@fullstaff.org. Please include your name and 
> specialty in the body of the email.
>
> --- On Mon, 2/4/13, histonet-requ...@lists.utsouthwestern.edu < 
> histonet-requ...@lists.utsouthwestern.edu> wrote:
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RE: [Histonet] Space requirements for AP lab

[Blazek, Linda]

LOL  I think if you have to ask then there isn't enough room!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Wednesday, December 12, 2012 3:37 PM
To: jmasla...@stpetes.org; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Space requirements for AP lab

Well, if it's more than 2.5 sq. ft per tech, we're out of compliance :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jmasla...@stpetes.org
Sent: Monday, December 10, 2012 12:41 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Space requirements for AP lab

Greetings All, Are there any JACHO or CAP space requirements for an AP 
laboratory? Looking for recommended square footage per tech, equipment, and/or 
work area.
Thanks


Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126 

Give thanks for ALL things.
"Kindness is the language the blind can see & the deaf can hear- Mark Twain 
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RE: [Histonet] H&E Question

[Blazek, Linda]

I second this one!  Been there!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich
Sent: Wednesday, November 14, 2012 12:35 PM
To: Goins, Tresa; Jaclyn Pitts; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E Question

I know this is out of the box, but it happened to me so I thought I'd pass this 
along. We had the same issue with our chief pathologist...hematoxylin was 
too light. We tried everything, but she still complained!! Then we diluted our 
Eosin Y 1:1 with 95% alcohol and then she was happy. Crazy, I know, but 
something to think about!

Best of luck, and please let us know your findings!
Lynette


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Goins, Tresa 
[tgo...@mt.gov]
Sent: Wednesday, November 14, 2012 11:02 AM
To: Jaclyn Pitts; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E Question

Jackie -

Try a different hematoxylin - we switched to Platinum Line Modified Harris 
Hematoxylin - we save money and the pathologists prefer the results.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts
Sent: Wednesday, November 14, 2012 8:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H&E Question

Hi All,
I am having a problem with my staining, ( well, I think its pretty good but the 
path says its not cark enough for her) So, I am using Azer Scientific 
Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 
5  min and then to 10 and 12 min. The problem is the control I have works and 
looks great at the 2.5 min. Besides just keeping it in the heme longer what 
else can I do? I have cut a few extra dummy slides today so I can try different 
things out with them. I am tired of hearing from the path everyday that the 
heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me to 
figure this out. Please help! Thanks!

Jackie
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RE: [Histonet] slide and cassette labeler

[Blazek, Linda]

Mary, 
Look into Primera Technologies.  They have a slide printer that would be ideal 
for your situation.  The distributer for them is Creative Waste Solutions 
http://cwsincorp.com/
Talk to Rex.  
We've had the slide printer for a while now and really like it.  Rumor has it 
that they may be coming out with a cassette printer in the near future too.
http://www.primerahealthcare.com/signature-slide-printer.html


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Fontaine
Sent: Monday, November 05, 2012 2:50 PM
To: histonet
Cc: Daniel Jones
Subject: [Histonet] slide and cassette labeler


   Our  hospital  will be looking into a cassette =d slide labeler. We
   are  a  small  lab  with  a  volume of about 4000 surgicals= would
   appreciate  any suggestions on the company and model that has workí
   will for others. This may be a stand alone saystem that is not hook up
   t=he hospital system .Thank you [1]mary.fonta...@vrh.org
   
References

   1. 3D"mailto:mary.fonta...@vrh.org";
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