[Histonet] Farewell
Hard as it is to believe - and I probably won't until I've been away for a week or more - this is my LAST day to work. Retirement? Who knew it would come around so fast? I remember having only fifteen years left to work and that was just yesterday! Histology has been 'bery, 'bery good to me and although I fell into it by accident, it has been a fascinating, involving, liberating experience. I'll be lurking on Histonet but under the alias of nmhisto and I'll probably do some p.r.n. work but I'm going home and throwing away my alarm clock and resetting the coffee maker for 6:00 a.m. instead of 3:45 a.m. Thank you all for your advice, guidance, humor, relative insanity and wisdom. Enjoy what you do and try to recruit at least one person into this field before it's your time to pick out the color of the tennis balls on your walker. Hail and Farewell and best of everything to every one of you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
Amen!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Block Tracking
During a recent inspection of my department, the inspector encouraged me to develop a method of tracking which control tissue I used for a case (i.e., individually identifying the block and referencing that identifier on the slide). To be honest, I believe this to be overload, but since I'm probably going to have to abide by that encouragement, I'd like to know if/how you identify which control block is used for a special stain. I can think of a simple method but wonder if any of you have used a really spectacular method that just blew the inspecting agency away. I'd promptly plagiarize it! Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Block Tracking, Part 2
Thank you to everyone for your tracking suggestions. I think we all agree that there are reasonable regulations and requirements for QA, and we can also agree that some are doing nothing but making our jobs more complex when it's really unnecessary. Soon, every laboratory professional will be attached to a tech robot - your clone physically! Every time you make a move, the docutech photographs and documents. At the end of the work day, you unplug your docutech and put him/her/it in your locker for recharging. I'm thinkin' there must be a movie in here somewhere - and it may not be a sci-fi feature. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Secret Identity
It just occurred to me (after prompting from a friend on Histonet who knows my Secret Identity) that you might not know who nmhisto@comcast is. That would be me. I'm transitioning to my home base and get Histonet there. That explains the strange posting from that person, does it not? But, yes, I'm still working - for now. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Removing coverslipping tape
Soak the slide in acetone for 10-15 minutes; it turns to something that looks like a slice of Jell-O and slithers down the slide, leaving cells and stain and structure in perfect order. I usually dip the slide in xylene a couple times to clear the acetone and re-coverslip it. Works like a charm. LOVE the coverslipping tape! I'm down to 20 days until I retire! Not that I'm counting or anything... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Interview Questions
Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Interview Questions
So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain Dye Stability Article
I've received permission to send a copy of the article from the Biological Stain Commission on Stain Dye Stability to those who asked. If you are one of those several individuals who asked me to send you a copy, please contact me directly and I'll attach and send to you ASAP. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain Dye Stability
I received a reply yesterday from an individual who attached a link to the article in Biotechnic Histochemistry relating to stability of dry stains. I have deleted that link but have had some inquiries from others about that article. Would the person who posted in response to my inquiry please re-post that link? The article was submitted by representatives of the Biological Stain Commission. Thank you! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain Dye Stability
For those of you who are patiently waiting for a link to the article, I am waiting for the person to re-post the link. If there is any way you might be able to access the article by searching online, it comes from Biotechnic Histochemistry 2009, 84(1): 11-15. Possibly there is some sort of link on the Biological Stain Commission website? In any event, I am hoping that the link will soon be posted for all. Thank you. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain and component shelf life
Can someone direct me to a reliable source for information on shelf life of stains and components, specifically expiration date for unopened vs. opened powdered/dry/granular stains? I have heard unopened dry stains w/shelf life of 10 years; opened dry stains w/shelf life of 5 years. Any citation for that? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Stain Dye Stability: The Reference
I received an excellent reference relating to shelf life of dye and stain powders and would like to post this as a reference for anyone having questions. The journal BIOTECHNIC HISTOCHEMISTRY 2009, 84(1): 11-15. I believe that this reference could stand the Credibility Test for accrediting agencies. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lillie, 3rd Edition
In honor of my impending RETIREMENT on April 1, 2012, I have decided to offer for sale my copy of Histopathologic Technic and Practical Histochemistry by R.D. Lillie, 3rd edition (1964). It is complete and in very good condition; pages unbent, minor page darkening (age) and comes with a FREE autograph in my maiden name. First offer of eighty dollars ($80.00) takes it and I'll pay the postage to get it to you. I've got to pay for my bon-bons and TV cable bill somehow! First offer gets it. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LILLIE
Well, you Crafty Individuals who found a 3rd edition Lillie at Amazon or the like and for cheaper, make it necessary for me to withdraw my offer of sale. I can't sell it for $8 and I'm not sure even about $40 (those online prices). No hard feelings. Deal's off. And I wasn't trying to pull a fast one. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cost of 10% NBF
I don't suppose that anyone out in HistoPersonLand has done a cost comparison between making your own 10% NBF and purchasing a 50-gallon drum of 10% NBF, perhaps? I did this several years ago but my idea of saving tech time/costs by buying the bulk was shot down because there was someone here that did the making. Now we're even more shorthanded and I think it would be a better use of everyone's time to just buy the darned stuff in bulk. Any chances that someone's already done this? If you have, I'd appreciate your input and my everlasting gratitude (brownies not included). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin Pricing Follow-up
Thanks to everyone who gave me Peer Review on my make-formalin-vs-buy-formalin. I appreciate everyone's input and with all the comments, I think I've convinced Our Fearless Leader to go with the 55-gallon drum idea. Thank you, everyone! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automated Slide Stainer and Coverslipper: Foil orGlass?
I know I've been around a l-o-n-g time, but what the heck is a foiled slide? That's a new one on me! Rats! Foiled again! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
Perfect!! And I was afraid Halloween was going to sneak by unnoticed! Which vendor sells holy water, 'cause I'm gonna need some... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
OOOH!!! Why didn't I think of that! Good goin'! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Luciferase IHC
At times like these, I wonder where Joe Nocito is... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Where are you, JTT?
This being Halloween Week, you must be lurking out there somewhere... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] SALARY
I nominate Dr. Cartun as the Patron Saint for Histopathology. We should work on a statue and a Mission Statement for him... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Difference between Neutral Buffered Formalin 10% andNBformaldehyde 3.7 to 4%
Now, wait just a minute! Don't tell me that after 40+ years of being a histotech that I've been WRONG all this time??? FORMALDEHYDE is FORMALDEHYDE is FORMALDEHYDE. FORMALIN is the diluted form of FORMALDEHYDE, buffered and used at (usually) 10%. If I'm wrong, this will be my last day at work... :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] formol
Not to put too fine a point on this interesting discussion (or lecture series, as it were), I add a little quote from R.D. Lillie, Histopathologic Technic and Practical Histochemistry, 3rd ed, 1965: The word 'formal' [sic], sometimes erroneously used as a synonym, refers properly to quite another substance, CH3O-Ch2-OCH3, dimethoxymethane. It matters not and I'm not even quite sure why I decided to take up the fight - what was the question??? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue processor
Although I appreciate individual opinions, do not overlook the Leica line. Us old timers may recognize the names Sakura or Thermo (in its many permutations) more readily, I'd put my money on Leica every time (and I have - with processor, embedding center, microtome and autostainer). They do not let me down. I have no issues with either other vendor, but I placed my trust in German engineering. I'm just sayin'... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Klotz Solution
I've just googled and histosearched for Klotz solution but cannot find anything that doesn't require chloral hydrate. Has anyone made a Big Breakthrough in making Klotz that can be made without that pesky controlled substance? Any help will be appreciated...who knew the recipe joined the Witness Protection Program! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nuclear Fast Red Substitute?
What can I use as a substitute for NFR for Prussian Blue iron stain, please? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] LEICA rep for New Mexico?
Will this person please contact me? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Mexico LEICA rep
I am trying to contact my LEICA rep for CONSUMABLES. Thank you to all who've replied so far... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] knife used for cleaning paraffin off the blocks
AHA! I have an Answer! EMS (Electron Microscopy Sciences) sells a One-Piece Scalpel Blade with Handle Cat. #72048-01 that is perfect for cleaning blocks of excess paraffin! I've had one for over 30 years and it will retire with me! It is initially sharp (but not as sharp as a normal blade would be) but if you cut open a few cardboard boxes with it (which it also does beautifully), it dulls down to the perfect edge for cleaning blocks. It fits the hand well and doesn't shave the plastic off the cassette. Someone left an old paring knife here but it's too big and cumbersome. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] repetitive wrist injury
I've just added DeQuervain's tendonitis to my repertoire! It joins bilateral carpal tunnel (repaired successfully) and trigger finger (two repaired, three developing). You'd THINK I could say it's time to quit but the boss has threatened to put sugar in my gas tank. Oh, bother... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Digital pathology systems
At a past NSH S/C, there was a vendor demonstrating digital pathology from the grossing station thru the slide. This was a system for photography of the gross specimen. Can someone remind me what company that was? I believe it was attached to a Thermo workstation. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Histonet Digest, Vol 92, Issue 35
I had this vision of your Blackberry in your back pocket --- well, you know... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica SELECTECH
Need to speak with a Technical contact regarding SelecTech staining system - someone who can give details about expiration dates. Cannot find contact info online. Thanks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab humidity range
We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way. Realizing that we all work in different environments and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report. If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm out of range. Please give me as much detail as you are able, without being required to join me in hiding. Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Humidididity Ranges
I love your replies because it gives me quite a range in order to avoid Incarceration, but I need to know THE CRITERIA (specific equipment operating parameters, for example?) you used to come to that range. I'm walkin' the plank here and I'm getting close to the end... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Remote Alarm for Processor
Do you use a remote alarm on your processor? If so, would you tell me what brand/model/manufacturer and how it works (keeping in mind that I am Electronically Challenged)? I run a Leica ASP300 in a new building with only digital phone lines and zero cell phone reception inside the building. I can't think of anything else that would complicate this issue more than that! Any help out there? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] What is a great manual microtomes
What she said! RM2235 without question! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OT: Wednesday Half-Wit
I used to use Sub-X in the processor. Changing paraffin was no problem because I'd just drain it into a Styrofoam box, allow it to set, seal it up, mark it waste paraffin and put it into the regular trash. Easy, right? Well - then I changed back to using xylene. First time I changed paraffin, I used my tried-and-true method, only to arrive at the lab in the morning to find a partially dissolved Styrofoam box on the counter, surrounded by a solidified pool of paraffin. Eureka! Xylene dissolves Styrofoam! It was not pretty but I think I've invented a new space-age compound. Fortunately, I was struck by an Inspiration - use an empty xylene/alcohol bottle with the top cut off (so I can insert the paraffin drain). I don't know why I'm posting this except to show that when confronted with an unusual circumstance, women rule! Now, back to work. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Methylene Blue
Okay - it's not for me, but I have offered to make up a new solution of NEW METHYLENE BLUE for our specimen prep area. I need a recipe for making an aqueous solution used to stain fluid preps from animals (urines, etc.). I've checked Lillie, Carson and Sheehan and Google. Can't seem to find an appropriate Formula. Help? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Retirement
It seems that my reference to RETIREMENT has gotten everyone thinking about it. Heh...heh.. It has been suggested that I reconnoiter in advance of Those of You Who Won't Be Retiring Before I Do (February 29, 2012, if the creek don't rise...). I would be happy to perform that hazardous duty but I need more of those $5.00 donations coming in for whatever it was that I posted last week (I hope my gray cells will rejuvenate when I retire). I won't need the money for travel because I think I'm right where I need to be. Have any of you thought about New Mexico??? Just within the past year, it has occurred to me many times why this is such a good choice for retirement. We do not have hurricanes, we do not have tornados (okay, maybe rarely), we are not prone to earthquakes, the weather is jolly darn good 90% of the time (spring is out - way too windy) and we don't have more than a couple or four inches of snow in the winter. We don't start our furnace/heater until November and it's only in use until maybe early April. The air conditioner was just put to use two weeks ago and we won't need it past the first part of September. Low cost of living, lots of homes (reasonably priced - info upon request) for sale and the number of things one can do in New Mexico are practically endless. We have everything but a beach (and if California keeps shaking, we might have beachfront property - not that I'd wish that on California...). We have skiing, a big lake (fondly called Elephant Butt [Butte]) for water activities, stream, river and lake fishing out the kazoo, mountains to climb, white sands in which to wallow, beautiful sunsets and terrible drivers. Oops - that one slipped out! The margarita (and Bud Light) are the State Drinks (if one is so inclined) and this is the Land of Manana (read it like Spanish). Manana is way much better than I needed that right this very minute and no excuses! Shaded patios, cool evening breezes and gorgeous cool summer mornings (at least until 7:00 a.m.). Besides, I need a replacement beginning March 1, 2012. Brand new lab, tech-designed, bright and LEED, tons of space, a separate storage room for blocks and slides AND a volatile storage room with two acid cabinets and two xylene/alcohol cabinets and a salary (that's another subject, I do work for a State...). Can't have everything, but this is darned close. And I do not work for the Chamber of Commerce! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ANATOMIC Pathology
With one exception (who shall remain nameless unless you want to send me another $5.00...), the Consensus of Professional Opinion Including the Samurai Pathologist (The Knower of All Things Histologic[al]) - I christen thee ANATOMIC Pathology. And thank you, all, for allowing me my leap across to the Land of Nonsensic[al]ness). I feel so much better for having caused a ripple in the cosmic continuum. Without further ado, I shall count my money and dream of that pathologist-less island (sorry, guys). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Retirement
Some of my compatriots are laboring under the assumption that retirement is sooner than later. If it were only true... my planned Date of Departure (pending an Offer I Cannot Refuse) is 29 February 2012. I have many ideas for interrupting your days until then... Fear not! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OT: How do you call...
I've figured out how to fund my approaching retirement! A poll! So... is the correct term Anatomical Pathology or Anatomic Pathology? Send $5.00 with your email reply and I'll have the down payment for that tropical island with internet. Seriously - what is the consensus and/or the correct usage? Figure I better get all these Deep Questions answered while I have the chance. (P.S., I'm only kidding about the $5.00 but I do take PayPal). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anatomicalalal
I just LOVE it when I'm right - and it happens so rarely! The consensus of ten responders so far is ANATOMIC. This just makes sense to me - we don't call them Diagnostical Pathologists, do we? Nah. Keep those correct answers coming my way - I needed the confirmation! And I have $50 toward the rowboat that'll take me to that island. Can you tell I need the diversion today? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] OT: How do you call...
Huh??? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Anatomicalalal
Okay - more money for my retirement fund! Is it Periodic Acid? Periodic Table of the Elements? Periodical Acid? Periodical Table of the Elements? Is Time magazine a periodic or a periodical? Ooohhh...this is fun. However, I've probably just about worn out my welcome in the OT portion of Histonet, so I should go back into hiding. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 10% NBF Shelf Life?
I have just come from the Histonet Archives (cough, cough...) and my Extensive Library of Histology Reference Books (Sheehan, Carson and Lillie) and have found nothing on the supposed or generally-accepted shelf-life of 10% NBF. Is there any such number? For example, we make our own 10% NBF in 20L carboys; our usage varies from day to day so sometimes it's used quickly and sometimes it sits for several weeks. It has been proposed to me that we add shelf life date to our carboys when a new batch is made. I know there is someone out there that has investigated this before and can give me a time and a reference (and I need the reference as well for completeness). Thank you so much - I'm standing by. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Yes - make sure they're long enough in the SEAT! Mine roll around the lab like little 5-wheeled rockets, but cut off the circulation to my thighs (is that something I can say on television?). And no arms on the chairs! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Von kossa stain
Funny you should ask... just last week I had two requests for a Von Kossa. That's when I found out that, despite the fact that we just moved into a brand new building with all the bells and whistles, there was not one single UV light in any of the many hoods that had been installed. Heaven forbid I could even find an incandescent bulb (we are a green building). So, I prepped my slides, put them in a clear glass Coplin jar and parked the jar on the hood of my car for an hour. Works like a charm. This all depends, naturally, on (1) if you even HAVE sunshine where you live; (2) how far a walk it is to the sunshine, and (3) whether you have a car hood on which to park the Coplin jar. Needless to say, out of the line of sight of curious onlookers with sticky fingers and no business wondering what that glass jar is... Nonetheless, it works just fine! May the Force be with you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] patient melanin pigment
Deparaffinize and hydrate to DH20. Place slides into 0.25% potassium permanganate for 30 min to 1 hour (visually inspect to measure level of melanin removed); wash in running tap H20 for 1 minute and place into 5% oxalic acid for 2-5 minutes until section becomes clear. Wash in H20 and stain with HE. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Off-Topic: April Fool's Day
What's the best April Fool's Day prank (not causing permanent damage, please) you've ever played on your pathologist? You can consider this a poll but the underlying reason is that I desperately need to do something to mark this occasion - this being my last year in the lab. I have three pathologists that are in serious need of some foolishness. The only thing that comes to mind is MASH's episode with the charcoal on the oculars of Colonel Potter's binoculars. I need serious foolishness. Please feel free to respond off-line to avoid giving away your secrets and causing you to be discovered for your past transgressions. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OT: April Fool's Nonsense
I like what I've gotten so far but I'm obviously a lot more evil than some of you... I'm thinking about how I could temporarily glue the radio station dial on the boss's radio to classical music in order to keep him from listening to Rush Limbaugh and Glen Beck. Or Saran Wrap over the urinal. Vaseline on office door handles. Filling one of their offices with bubble wrap. Gluing their desk drawer shut (the one with the food in it). Let's get SERIOUS here - I need more! I want people to be glad I've retired! Heh...heh... No pathologists were harmed in the making of this Tomfoolery. Darn it. And, yes, I do have work to do today... so far, anyway. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] End of April Fool's Day
I've collected enough fodder to take me through the year 2015 - good thing I'll be retiring before that or I'll be surplused! Thanks to everyone who contributed and if you have anything further, send it to me directly so we don't clog up Histonet any more than I already have. I'll post a comprehensive list of suggestions that I've gotten... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] April 1st Fun
Dead cockroach on autopsy/necropsy table as legitimate specimen; goldfish processed as patient Barry Cuda; change family photos to total strangers; butterscotch pudding in (unused) specimen jar and subsequent tasting with applicator stick; activated charcoal on oculars (a la Colonel Potter in MASH); single hair attached to ocular with super-glue (tickling nose); cut fingertips off gloves; pumpkin/squash in animal shapes to slide; bug in block; foam-rubber-stuffed-box-with-toy-rat-to-burst-out-and-cause-startlement (toy rat to morph into histo lab mascot complete with appropriate holiday costume); process beef jerky as legitimate specimen; light coating of oil on 'scope oculars; - and the latest one I just received - a small slip of paper on the underside of the (computer's) mouse. Oh - the joy! What shall I do first?! Happy Annoying! This ought to cover the last several months I've not done the Friday Hour of Fuming... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Processing animal fat
How about a little time in 10% neutral buffered alcoholic formalin? Say a couple hours or more? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] glass coverslippers
I agree - I have a totally Leica lab except for my Sakura tape coverslipper and I would not trade it for anything. I test-drove the Leica glass but it seemed that there were more minute (my-noot) adjustments than for the Space Station and I could see problems down the line (sorry, Leica!). I know some love tape and some hate it but it's the absolute easiest to use and adjusting for drip (xylene) and length of coverslip (for those pesky Ventana-labeled slides) is a breeze. My bosses have no issues with optical quality or photography with the tape, either. Tape: Hoo-rah! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Daylight Savings Time?
Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathco double-blade handle
Am looking for this item to replace one that was older than I am. I need the handle (perhaps Pathco #D2877) for the blades (Pathco #D2878-2C). The catch is that I want the SOLID BRASS handle - not the aluminum. I've googled all over the place - can anyone help me find these handles? Muchas gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bacterial Contamination
Thanks to everyone that sent in their suggestions. I especially liked the one that suggested I inform all my pathologists that special stains are unconstitutional. Seriously, though, I'll give all your suggestions a shot and see if I can get rid of the little rascals. Thanks again! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Go for HISTOLOGY!! Cytology requires a lot of Sitting in One Place Staring into a Microscope (please! - no offense to cytotechs - really!). With histology, your daily routine is varied and invigorating. And now I'm probably on some Cytology Tech Blacklist... sigh... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Static issues
This is New Mexico where humidity is a rumor. The humidity in the lab here - as I write - is 18% and that's on a really wet day! If I have static issues with my ribbons, I just lean a little bit toward the block and breathe on it and the ribbons just float (in a good way) off the knife. I do that so often that when I use my sewing machine, I find myself breathing on the material. That's just sad! But try the Breathing Thing. Or not. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Stupid, stupid static!!
This has SO many possible responses but I'll not go there. Someone might videotape it and leak it to the press... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Light Green
Why does my light green counter stain just stain the edges of the sections??? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Veterinary SOPs
For those of you who work in a veterinary diagnostic lab where there is a BSL-2 and/or BSL-3 necropsy room, I could use some assistance with designing some SOPs for maintenance of those rooms. For example, what are your daily routines and/or procedures to ensure that those rooms are clean, temperatures checked, setup requirements met, and so forth. This is not writing pathologists' SOPs for specific necropsy procedures, but more the day-to-day operation and maintenance of the rooms in which a pathologist would work. I would appreciate any input and will share with those who ask. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processor change
I change solutions after 500 blocks; paraffin after 1500 blocks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtomy SOP
Thanks to everyone (seven of you!) that sent me examples of your microtomy SOP. I'll pick and choose from all to build my own - and it will save a lot of time, I'm positive. I tend to make my SOPs rather generic as far as specific steps, equipment names, etc., at the behest of my QA manager. We'd rather not be too specific in case momentary deviation is required - that's a fine line, let me tell you! Thanks to my Faithful Contributors and have a wonderful Thanksgiving holiday. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtomy SOP
I am sorry to say that as I received the SOPs on Microtomy, I printed and deleted them. I apologize. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOP for Microtomy?
Polishing up my List of SOPs To Write. Don't want to bake from scratch if there's an instant mix (that only applies to writing SOPs, by the way). Do you have an SOP for the physical process of microtomy (i.e., cutting a paraffin block) that I could plagiarize? I will trade a pre-written SOP on Microtomy for a one-way ticket to your closest city on a glass-bottom bus. Muchas gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] lost tissue
Gremlins. That's what it is. I recently cut in a case with two parts - one Extremely Thin Black Crescent of tissue in one cassette and the other tissue in the next cassette. The next morning, the Extremely Thin Black Crescent was NOT in the cassette. Gulp. Sometime later, as I was almost done embedding, I opened a cassette and there - lo and behold! - was the Extremely Thin Black Crescent. Creepy. I had the pathologist confirm that it was, indeed, the Extremely Thin Black Crescent of tissue that had started out somewhere else... 'splain that, Lucy! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I'm in the New Lab!
We are physically moved and Organized Chaos prevails (except in Histo, of course!). There's been some discussion about what would be needed when designing a new lab and I agree with what has been offered - and actually used this sort of input from Histonet, along with the lab design course at NSH (which was priceless), and visiting a couple of labs before meeting with the architects. It was a long and arduous process but, with the exception of the chairs chosen by the majority in this building (about 700 people), was absolutely worth all the pain and agony. Please make note of our new physical address and a direct line to the histo lab (which I'll answer just as soon as I figure out how to work all those buttons and knobs!). Now to find out where I set up the microtome so I can start cutting! Good day, all! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Metal Base Molds
Try Electron Microscopy Sciences ( www.emsdiasum.com). They are a treasure-trove of goodies like base molds. They have things in their catalog you didn't know you needed until you see it! I just looked and they have many sizes of base molds. Shop! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cool Formalin
Thanks to everyone that replied to my question about refrigerating 10% NBF for up to 6 weeks. I'll fold your information into the decision. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Refrigerate formalin?
We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chilling trays
I went to eBay and found a set of three (total $12.00) stainless steel food prep trays, roughly 9x5x2 (LxWxH). I fill them with DI water and freeze them. I also inherited a larger stainless steel container that's 12.5x10.5x4.5 - same deal, DI water and freeze. I face blocks, put them on the ice and add a little DI water and soak for 10-15 minutes - blocks cut like a dream! They stay frozen for a l - o - n - g time! Try a local restaurant supply. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] State Veterinary Diagnostic Labs
Are there any other State vet dx labs besides Minnesota, Kansas, Ohio and Illinois that would like to participate in my Poll about Ventana service contracts? I need more input! Thanks! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vet Dx Labs
If you are the tech for one of the States' vet dx labs, could you please contact me; I have a question about the Ventana NexES and the service contract ending. I don't want to send out to the NVSL list because my list is from 2003 and I'm sure it's changed. Once I have all your names, I'll send out one email with one question about the NexES. Thanks! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Waterbath Options
Okay, time for my two cents' worth. Water baths - I don't like the glass-dish-insert kind because water can get beneath the glass dish and can temp fluctuations. And if the glass insert breaks, it might be difficult to find a replacement (Pyrex?). I like the good ol' fashioned round or rectangular black-lined fill-it-with-water-and-turn-it-on kind. They hold temp much better and because they are lined with something like black Teflon (or whatever), they clean like a dream. I found that I really do not like the romantic under-tissue lighting. It's all about preferences and this is mine...not that you asked, but that never stopped me before, did it? Happy Tuesday! Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Linda Blazek
Please contact me at this email address. Thank you. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 595-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sticky knives
Some time back, I posted a message here about how my Feather blades were sticking together and hard to dispense. A nice Sakura person contacted me to offer help but I'd just opened a new dispenser of blades and they were just peachy, so I replied to her that all was well. Well, not entirely true - could that nice Sakura rep please contact me again so I can discuss her offer of help? Thank you. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Theresa Schuldt?
If Theresa Schuldt is out there, please contact me. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Communication when pathologist are not in same area aslab
Communication? I'm just down the HALL and we don't communicate! :) Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Storage Boxes
One of my former labs used plastic stacking boxes for holding blocks between cutting and filing, as tool trays and for many other purposes. These are the same boxes used in eyeglass fabricators (LensCrafters, etc.). I've been searching for them for quite some time and have just found a supplier at www.westernoptical.com (and likely other optical suppliers as well) - search for plastic stackable shop trays. I just bought a dozen for $30. I find them indispensible, which is why I've been looking for them for so long. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HISTO STORIES
I have sent out the collection of histo stories but need email from each one of the following people: greenjumpyone, Thom Jensen, Valerie Rodriguez, Sharon Campbell, Mary Abosso, Janice Mahoney, Valerie Hannen, Valantou Grover, Glen Dawson, Bernice Frederick, Sharon Beckham. When I forwarded emails posted to Histonet to my home email, all addresses showed on behalf of... and not your actual email address. Send them to me here at work today and you'll get immediate gratification (a copy of the Stories). If you did not request a copy but would like one, email me and here it'll come! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] For Warren Eddings
Warren, please send me another email address other than the one you just used to request a copy of the Histo Stories. I'm getting an error that will not allow it to be received at your end of the wire. Thanks. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Stories - Send Yours
I'm typing like a madwoman and am up to about 6 pages of single-spaced stories with at least 25 more contributions to add. This is a very interesting read and I want to make sure everyone has a chance to Tell Their Tale. If you would like to add your story to the collection, please send it to me before the beginning of next week so I can meet my self-imposed deadline of getting it done and distributed to you around April 15th. PLEASE SEND TO MY HOME EMAIL: nmhi...@comcast.net - not to Histonet so we don't clog up the mechanism. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Stories
To all of you who sent me your how did I get started in histology stories, I have not forgotten you! I am making all your contributions into one document and although I have about a dozen left to transcribe, if you will contact me DIRECTLY at nmhi...@comcast.net, I will send you the entire document when I finish it. Taken as a whole, it's pretty amazing/interesting/involved/riveting! Please do NOT contact me via Histonet - use my home email as above. My plan is to submit the stories to NSH to use as a tool for recruiting (it seems that most of us got our start through karma!). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Stories, Part 2
PLEASE send requests for the histo stories to my HOME EMAIL ADDRESS - not via Histonet!!! nmhi...@comcast.net. Thank you! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Question
I have my processor set to notify me at 1500 blocks, at which time I change the paraffins. I usually run 500 in the other solutions (same notification system) before changing. It works for me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Silver Lips and Fingers
I'm not a chemist and may shoot myself in the foot here, but if gold chloride tones silver, would it work on skin? Then you could call it a beauty spot? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Costing Thanks
Thanks to those of you who sent me Magical Spreadsheets for Calculating Histo Costs. I highly recommend the one that Tim Morken did for a presentation at 2007 NSH - it is excellent and covers every conceivable scenario and allows you to calculate anything. I hesitate to forward it without his permission, but I'm certain you could contact him directly and ask for a copy. It's priceless (as in worth the price of admission). Thank you to all. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cost per slide?
Salutations (that's my real name) to all of you in the VETERINARY DIAGNOSTIC world. I need to come up with the cost of producing one HE slide and because I'm Old and Lazy (not), I'm wondering if any of you have already done this complicated calculation and have a number I could use. I barely have enough time to gross in, type reports, embed, cut, stain, coverslip, file slides/blocks and do special stains and immunos as it is! I shouldn't claim I'm lazy but why invent the wheel twice, I'm sayin'. I'd appreciate any cost you might have... Muchas gracias! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Accessioning Similar Samples
Regarding the need to avoid accessioning similar samples consecutively, I addressed this last week with my bosses. Specifically, I asked that spleen specimens not be accessioned consecutively (because of shattering and static and the possibility of fragments being carried over); my response was that I should just not CUT the blocks consecutively. Not the point I was trying to make but typical in some cases when addressing the issues histotechs face, true? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Stories
Thanks to everyone that sent their Story of How I Ended Up Doing This Histology Thing! I have gotten 50 or more replies! The one thing that strikes me is how many of us went into this profession without a clue! With all the opportunities to recruit future histologists, this Histology Day idea is a good start. On the original subject, I'm planning to make one document out of all the replies and - WITH PERMISSION - attach your name to the answers. If you do NOT want your submission listed because you want to remain anonymous, you must let me know ASAP. Send to: nmhi...@comcast.net. Thanks for your stories! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Stories, Part 3
I am stunned and amazed at the amount of response this little query has gotten! I have forwarded those that were posted here to my home email (and I've gotten many there, too) and expect that I'll get many, many more. I know not everyone has time to respond to email every single day, so I'm giving it more time. Unless you specifically request to remain anonymous, I'm going to give each contributor credit for their story. And because we have so many members from around the world, I would very much like to know how our fellow techs got started in histology in their countries. But this whole endeavor has been very interesting and I thank each of you for sharing your story. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Professionals Day
If it weren't for a med-tech-turned-pathologist that took me up on my interest in becoming trained in histology (1967), I would not have just marked my 41st year as a histologist. Back then, you just had to have OJT and study independently for your written and practical tests. I had no idea what I was getting into but it was one of the absolute best choices I ever made. To pretend to quote some baseball player, Histology been berry, berry good to me! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Professionals Day
Perhaps what each of us ought to do is write a quick line naming the one person that got you involved in histology and how they did that. I'd be willing to gather them and pass them on to NSH so they could post it at the Seattle meeting. If you'd like to do this, send the email to nmhi...@comcast.net and I'll collect. Just an idea... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcohol followup
As I pressed the SEND button it came to me like a bolt of lightning that alcohol is GRAIN based. Oops... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
