[Histonet] retracting or non-retracting microtome?

[Cazares, Ruth via Histonet]

Good morning all,

Which is the best rotary microtome for routine tissues and biopsies?  No 
plastic only paraffin.  I am leaning toward retracting, but is there a benefit 
to non-retracting microtomes?

Thanks in advance:)


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 N. California
Chicago, IL 60625
(773) 878-8200 ext-5190


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[Histonet] I have an open part time position

[Cazares, Ruth via Histonet]

Good morning Histonetters!

Swedish Covenant Hospital in Chicago has an available 20 hour per pay period 
position. The hours are 8 pm to midnight, 2 days one week and 3 days the next 
week.  The days are shared with a second histotech and the Fridays are 
alternated.  You would be responsible for cutting biopsies and any immunos, 
special stains or deepers ordered after the techs have left.

If anyone is interested, please apply by following the link below.

https://recruiting2.ultipro.com/SWE1003SCHMC/JobBoard/b3bbfb25-ba1f-43ae-a6cb-4f0e2cb70043/?q=hist=relevance

Any questions, please feel free to contact me directly.


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 N. California
Chicago, IL 60625
(773) 878-8200 ext-5190
rcaza...@schosp.org


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[Histonet] histology temporary help

[Cazares, Ruth via Histonet]

Hi all,

I am looking for temporary help for the summer, 13 weeks from end of July to 
the end of October.  Early morning 6 am to 2:30 (or 5am to 1:30 pm).  If anyone 
is interested or knows someone, please have them fill out the online 
application for Swedish Covenant Hospital  (Chicago).

OR... Does anyone know of a temp agency that staffs Histotechs and is local?


Thanks in advance!!

Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 N. California
Chicago, IL 60625
(773) 878-8200 ext-5190


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RE: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - Email found in subject - Email found in subject

[Cazares, Ruth]

Hi William,

Our competency evaluation is done once a year as part of the employee's annual 
evaluation.  I don't actually have each employee perform the procedure as part 
of the documentation, although. h, maybe an in-service to 
discuss the new procedure with all employees?

I agree with you about discussing things on Histonet, I have found it to be a 
lifesaver on more than one occasion (OK, so maybe that's a little dramatic, but 
you get the point) :)


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
Chicago, IL





From: WILLIAM DESALVO [mailto:wdesalvo@hotmail.com]
Sent: Thursday, December 23, 2010 10:50 AM
To: Cazares, Ruth; histonet
Subject: [SPAM-HC] - RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding 
procedure manual - Email found in subject - Email found in subject

Thanks, I am aware of Peggy's form. We have similar hard copy forms now, but we 
are moving to an electronic system. It will be much more manageable for 125 
employees.

When you state in your form the employee is competent, do you have 
documentation to support that there was a competency evaluation? We only state 
the employee has read and understands. We have separate documentation for 
training and competency.

I love this type of discussion string. There are so many different processes 
out in Histoland.

Do others document separately the original creation, training, competency, 
implementation of procedures/processes and then continual yearly training, 
competency and review of those processes? Are you using a Quality Management 
System? How about Electronic system to manage?

William DeSalvo, B.S., HTL(ASCP)





 From: rcaza...@schosp.org
 To: wdesalvo@hotmail.com
 Date: Thu, 23 Dec 2010 10:17:48 -0600
 Subject: RE: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure 
 manual - Email found in subject

 Hello histonetters,

 When I implement a new procedure in my lab, besides the routine yearly 
 sign-off sheet that accompanies the procedure, there an additional sheet that 
 states

  The following employees have reviewed this procedure and by signing below 
 are acknowledging that they understand and are competent in performing the 
 procedure:

 Below that statement is a box with signature and date lines for all the 
 employees using this procedure to sign. This makes each employee responsible 
 for reading and understanding the procedure. This I got from one of Peggy 
 Wenk's workshops (Thanks Peggy!!). I can send a copy to anyone who'd like 
 one, just contact me directly.


 Ruth Cazares, HT (ASCP)
 Histology Supervisor
 Department of Pathology
 Swedish Covenant Hospital
 Chicago, IL





 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM 
 DESALVO
 Sent: Thursday, December 23, 2010 7:27 AM
 To: bsulli...@shorememorial.org; Victor Tobias
 Cc: histonet; histonet-boun...@lists.utsouthwestern.edu
 Subject: [SPAM-HC] - RE: [Histonet] CAP Question regarding procedure manual - 
 Email found in subject


 Beatrice,
 How do you show proof to CAP that your employees that use the procedures and 
 perform the tasks described in the procedures have reviewed and understand 
 the procedure when there is a new one during your cycle year and before it is 
 implemented?

 CAP has changed the way they inspect and they are now looking for how whether 
 the employees performing a task described in the procedure understands the 
 procedure and is performing exactly as the procedure describes. They are not 
 as concerned that the Supervisor and management knows about the procedures, 
 they want to see how informed, trained and competent the bench employee is.

 William DeSalvo, B.S., HTL(ASCP)





  To: vic...@pathology.washington.edu
  From: bsulli...@shorememorial.org
  Date: Thu, 23 Dec 2010 07:53:22 -0500
  Subject: Re: [Histonet] CAP Question regarding procedure manual
  CC: Histonet@lists.utsouthwestern.edu; 
  histonet-boun...@lists.utsouthwestern.edu
 
  Victor,
  To my knowledge all you need is proof that the staff reviewed the manuals.
  We accomplish this by a sign off sheet in the front of each manual we use.
  The Supervisor, or designee, needs to review and sign off on each procedure
  in each manual.
 
  Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
  AP Supervisor
  Shore Memorial Hospital
  609-653-3590
 
 
  Speak only well of people and you need never whisper
 
 
 
  Victor Tobias
  vic...@pathology
  .washington.edu To
  Sent by: Histonet
  histonet-bounces@ Histonet@lists.utsouthwestern.edu
  lists.utsouthwest cc
  ern.edu
  Subject
  [Histonet] CAP Question regarding
  12/22/2010 06:16 procedure manual
  PM
 
 
 
 
 
 
 
 
 
  Is there a requirement to have a signature of every staff member on a
  procedure if they perform that procedure in a manual? Wouldn't one
  signature on a cover page suffice that you have read

RE: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - Email found in subject

[Cazares, Ruth]

In the summer of this year, ASCO/CAP issued new guidelines for proper handling 
of breast specimens to improve the accuracy of ER and PR status. It states that 
the fixation of breast specimens must extend for at least six hours and no more 
than 72 hours. It stands to reason that these guidelines should also stand for 
Her2 testing. On the second to the last paragraph before the authors disclaimer 
it reads,

We are confident that these guidelines and measures developed for testing of 
ER, PgR, and HER2 will improve performance of laboratories using these and 
future predictive testing methods.
You can find the guidelines at this website, click on FULL TEXT (on the right).

www.asco.org/guidelines/erpr

Comments? Opinions? Anyone?

Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
Chicago, IL



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
teri.hall...@midmichigan.org
Sent: Tuesday, October 12, 2010 11:57 AM
To: janice.maho...@alegent.org; mpe...@grhs.net; joyce.cl...@wchsys.org; 
histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR - 
Email found in subject

Sorry Jan,

ASCO/CAP Guideline Recommendations for HER2 in Breast Cancer Article
Table 6. Sample Exclusion Criteria to Perform or Interpret a HER2 FISH Assay
Item #3 Tissue fixed for prolonged intervals in formalin (greater than 48 hours)

Teresa Hallada MTCT (ASCP)
MidMichigan Health Gratiot



From: histonet-boun...@lists.utsouthwestern.edu on behalf of Mahoney,Janice A
Sent: Tue 10/12/2010 12:05 PM
To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR



Formalin fixation time does not impact the results of FISH as it does IHC.
Jan M
Omaha

-Original Message-
From: Mike Pence [mailto:mpe...@grhs.net]
Sent: Tuesday, October 12, 2010 11:00 AM
To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

I don't think it matters if you do Her2 by FISH or IHC the time is still
48hr. I hope I am wrong, but I don't think I am.

Mike

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Tuesday, October 12, 2010 10:25 AM
To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR


We have decided to reflex to FISH those breasts that do not fall within
the recommended formalin fixation time.  We do work on Saturdays so it
is only the rare 3 day weekends that this comes into play. Jan M Omaha

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: Tuesday, October 12, 2010 10:10 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR

Does anyone have any experience with storing formalin fixed breast
tissue in 70% before processing?  I am trying to comply with the new
guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and
since my lab does not operate on the weekend we have been well above the
48 hour recommended formalin fixation time.  Does 70% affect
antigenicity for either Her2 or ER/PR?  Any information or suggestions
will be greatly appreciated.  Thanks :)


Ronda Souders
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
ronda.soud...@wchsys.orgmailto:ronda.soud...@wchsys.org


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RE: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject

[Cazares, Ruth]

Hello Histonetters,

I have just received the microtome aligner tool from TechOne that Newcomer has. 
 I have used it on my three microtomes and let me tell you guys, I LOVE IT!!  
It is very easy to use, just follow the instructions that come with it. I 
strongly recommend it (but that's just my opinion).



Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
Chicago, IL



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Wednesday, July 14, 2010 10:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - [Histonet] Microtome Aligner - Email found in subject

Hi All, I just want to clarify that the microtome aligner that we are
having issues with is the one made by Advanced Innovations and sold by
Newcomer Supply not the one made by Tech One (Shane Perkins) which
Newcomer also sells.  I have had no experience with the device that
Shane is referring to.



Joanne Clark, HT

Histology Supervisor

Pathology Consultants of New Mexico

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RE: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject

[Cazares, Ruth]

Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to 
processing. My techs say they cut like butter!


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba
Sent: Tuesday, April 27, 2010 5:22 PM
To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found 
in subject

HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide!

(1) Cut the slides and place on Plus(+) slide
(2) shake off excess water
(3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at 
bottom.
(4) place in hot oven for 5 mins (50-60 degrees)
(5) remove from Coplin jar under a hood as formalin drops are now carcinogenic 
vapour
(6) place slides in rack in oven so that the formalin moisture dries...
(7) slide is ready for use

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
zodia...@comcast.net
Sent: Tuesday, April 27, 2010 12:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] toe nails falling off slides

To all,


I was wondering if anyone out there had any tips on how to keep toenail 
sections from falling off slides. We mount them on positive slides, air dry for 
about an hour but they still fall off. Any insight on the subject would be 
greatly appreciated.


Thanks,


Jenny
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RE: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject

[Cazares, Ruth]

Kim wipes work great, and if done after each block shouldn't slow things down 
much. Besides, what's the point of quantity when the quality is compromised 
with floaters. You should have a policy regarding this since it is a CAP 
requirement.


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles
Sent: Friday, October 23, 2009 9:11 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - [Histonet] Floaters in Waterbath - Email found in subject

I know we have all had some problems with floaters in our waterbath at some 
point in our microtomy career.
Our doctors are very picky and I need some tips on keeping an immaculate clean 
waterbath, but not sacrificing the speed in a regular routine lab.  We use the 
pyrex waterbath and paper towels for wiping our area.

Thanks
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RE: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject

[Cazares, Ruth]

 I gave out misinformation which I want to rectify.

The metal sleeves and holding tray is sold by McCormick Scientific which is a 
part of Leica, but they have their own ordering website:

http://www.mccormickscientific.com/cassettemangement.asp?PCID=853

The above link should open to the item itself. The ordering information is-

# MC 100  Formalin Holding tank
# MC 101  Formalin Tank Lid


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: Cazares, Ruth
Sent: Wednesday, August 26, 2009 4:22 PM
To: 'ba...@gundluth.org'; histonet@lists.utsouthwestern.edu
Subject: RE: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - 
Email found in subject

Hi Barb,

We have metal sleeves in which 18 cassettes fit standing front to back, and a 
metal box with lid which we fill with formalin. This box holds 6 sleeves and as 
cases are grossed, the cassettes are picked up in order (it doesn't take any 
effort to do this at the time of grossing) and placed in the sleeves. We have 
two metal box/containers in which we separate our routines and our biopsies (we 
use a short program for our biopsies). At the end of the day we print out hard 
copies of all the cases that will be cut the next morning, separating the 
routines from the biopsies so there are 2 lists, and then we go cassette by 
cassete and check off on the list to assure that every cassette is accounted 
for.

This system works great for us and it helps to find errors before they go any 
further. We use colored dots on the cases that are biopsies so when its time to 
print out our log sheets we can do so from the requisitions or working log 
sheet, and if done right, every case and cassette should match up.

I believe Leica now carries these sleeves and metal box containers, I strongly 
urge you to look into them, they keep things so organized and we LOVE them!! I 
can get you ordering information, just let me know.



Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
ba...@gundluth.org
Sent: Wednesday, August 26, 2009 3:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email 
found in subject


Hi all -

For those that do batch overnight processing, how do you organize the cassettes?

Currently we have 2 path assistants that gross throughout the day, and each 
puts their cassettes as they are grossed into a bucket of formalin.  At the end 
of the day a histotech drains the formalin off, rinses the cassettes in water, 
then manually puts the cassettes into order according to our worklist, with 
rush cases being put up front.  The baskets are then loaded onto the tissue 
processor (Sakura VIP5 and VIP6).

We are wondering if there are some other ideas of how to streamline this 
process.  One thought was to have the cassettes loaded/organized into the 
tissue processing basket as they are grossed, but have a concern about formalin 
exposure while doing this.

 Any thoughts would be greatly appreciated.

Barb Moe
Gundersen Lutheran Medical Center
La Crosse WI


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RE: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email found in subject

[Cazares, Ruth]

Hi Barb,

We have metal sleeves in which 18 cassettes fit standing front to back, and a 
metal box with lid which we fill with formalin. This box holds 6 sleeves and as 
cases are grossed, the cassettes are picked up in order (it doesn't take any 
effort to do this at the time of grossing) and placed in the sleeves. We have 
two metal box/containers in which we separate our routines and our biopsies (we 
use a short program for our biopsies). At the end of the day we print out hard 
copies of all the cases that will be cut the next morning, separating the 
routines from the biopsies so there are 2 lists, and then we go cassette by 
cassete and check off on the list to assure that every cassette is accounted 
for.

This system works great for us and it helps to find errors before they go any 
further. We use colored dots on the cases that are biopsies so when its time to 
print out our log sheets we can do so from the requisitions or working log 
sheet, and if done right, every case and cassette should match up.

I believe Leica now carries these sleeves and metal box containers, I strongly 
urge you to look into them, they keep things so organized and we LOVE them!! I 
can get you ordering information, just let me know.



Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
ba...@gundluth.org
Sent: Wednesday, August 26, 2009 3:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [SPAM-HC] - [Histonet] Organizing of cassettes for processing - Email 
found in subject


Hi all -

For those that do batch overnight processing, how do you organize the cassettes?

Currently we have 2 path assistants that gross throughout the day, and each 
puts their cassettes as they are grossed into a bucket of formalin.  At the end 
of the day a histotech drains the formalin off, rinses the cassettes in water, 
then manually puts the cassettes into order according to our worklist, with 
rush cases being put up front.  The baskets are then loaded onto the tissue 
processor (Sakura VIP5 and VIP6).

We are wondering if there are some other ideas of how to streamline this 
process.  One thought was to have the cassettes loaded/organized into the 
tissue processing basket as they are grossed, but have a concern about formalin 
exposure while doing this.

 Any thoughts would be greatly appreciated.

Barb Moe
Gundersen Lutheran Medical Center
La Crosse WI


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[Histonet] Prostate needle saturation biopsies

[Cazares, Ruth]

Hello Histonetters,

With the new Medicare G codes for saturation biopsies of the prostate, the 
flyer reads:

G0416, Surgical pathology gross and microscopic exam for prostate needle 
saturation biopsy sampling, 1 to 20 specimens.
G0417, 21 to 40 specimens.
G0418, 41 to 60 specimens.
G0419, greater than 60 specimens.

We seem to be having a difference of opinion as to how we should use these new 
codes.  Some of us think that if we get forty cores both G0416 and G0417 should 
be used. Some think only G0417 should be used and some just don't know. 
Medicare was no help, they said Write a letter.

How are others out there using these codes?


Ruth Cazares
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital


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