I have perfused mice and rats at 300 mm Hg, about double physiological
level, don't know what that made the flow rate. All mammals have the
same blood pressure (within tolerances), so it is easier to select a
suitable pressure to use than a flow rate, which varies dramatically.
I look at brain, never pay any attention to the gut. Clear fluid comes
out the nose, that is a good sign. There are pressure release valves
across the cribiform plate to release CSF if there is too much. I am
flooding the system, fluid coming out the nose means the extracellular
fluid and CSF is being replaced as well as vascular blood. Good.
The tissue is quality is excellent, free of red blood cells, can be
unshrunk depending on the tonicity (should be sub isotonic) of the
fixative fluid. Have looked at Nissl and EM material, no evidence of
damage to the tissue.
If gut is extended, might have something to do with the large intestines
job of removing fluid from feces, and flooding the system swells the
tissue. But does it matter? Do you use that tissue? What is the
tissue quality if you use it after physiological pressure perfusion.
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com http://www.myneurolab.com/
www.leica-microsystems.com http://www.leica-microsystems.com/
IMPORTANT - This email and any attachments may be confidential. Any
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From: Merced M Leiker lei...@buffalo.edu [mailto:Merced M Leiker
lei...@buffalo.edu]
Sent: Monday, March 29, 2010 9:05 AM
To: Joseph Saby saby_josep...@yahoo.com;
charles.scou...@leica-microsystems.com; mak...@ufl.edu;
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mouse perfusion rate
Hi Joe,
Thanks for that notice about flow rates. But I think for the mouse you
meant 1-3mls/min (not per 10min?)...
Regards,
Merced
--On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby
saby_josep...@yahoo.com wrote:
All-
From previous work with rat perfusions, the flow rate was about 10
ml/minute. If I had to guess, the equivalent flow rate for a mouse
would
be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will
definitely cause blowout artefacts.
Joe Saby, BA HT
__
From: Merced M Leiker lei...@buffalo.edu
To: charles.scou...@leica-microsystems.com; mak...@ufl.edu;
histonet@lists.utsouthwestern.edu
Sent: Fri, March 19, 2010 9:21:38 AM
Subject: RE: [Histonet] mouse perfusion rate
The vasculature will leak too much and the mouse will get bloated -
you'll
see it first in either the intestines blowing up like a balloon or
fluid
coming out of the nose. Just not the same as the heart pumping when
the
mouse is alive with intact physiology and normal functioning. Don't
know
exactly why, but that's what happens when you go too fast. Perhaps the
vasculature has lost its control to compensate for the pressure? I'm
not
a
physiologist so I'm not sure why...maybe someone on the Histonet can
answer
that?
Regards,
Merced
--On Thursday, March 18, 2010 5:49 PM -0500
charles.scou...@leica-microsystems.com wrote:
Why not? What happens? One would think the mammalian cardiovascular
system could withstand physiological pressures and flow rates, at
least
for one lifetime?
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com
www.leica-microsystems.com
IMPORTANT - This email and any attachments may be confidential. Any
retransmissions, dissemination or other use of
these materials by persons or entities other than the intended
recipient
is prohibited. If received in error, please contact
us and delete all copies. Before opening or using attachments, check
them
for viruses and defects. Our liability is limited
to resupplying any affected attachments. [Any representations or
opinions
expressed in this email are those of the
individual sender].
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Merced M
Leiker lei...@buffalo.edu
Sent: Thursday,
