Re: [Histonet] prostate needle bx

[Cristi Rigazio via Histonet]

Hi Silvia,

We process them with the rest of our small stuff.  About 3 hours on a Peloris.  
We get them and process them on an ASAP run to embed cut stain and distribute 
overnight to be on the pathologists desk at 8.

> On Feb 2, 2023, at 7:31 PM, Silvia Bonner via Histonet 
>  wrote:
> 
> Hi Everyone,
> 
> What are the different processor run times for prostate needle biopsies? We 
> might be processing them and I wanted to get an idea of schedules and average 
> turnaround time.  Are you doing the same day? Overnight?
> 
> Thank you,
> 
> 
> Silvia Bonner, BS, HT(ASCP) CM
> 
> Histology Supervisor
> 
> 
> 
> sbon...@pathregional.com
> 
> Pathologists’ Regional Laboratory
> 
> Anatomical Pathology
> 
> 2841 Juniper Dr. Suite 1
> 
> Lewiston, ID 83501
> 
> (208)746-5533
> 
> 
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> This email may contain physician, patient and/or attorney material that is 
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Re: [Histonet] Post a question

[Cristi Rigazio via Histonet]

Good afternoon,
We are set up the same way, but we have a pathologist located at each location. 
 We do have a mix of on site and courier services.  When we transport to the 
hub for crossing we use a large cooler.
Thanks
Cristi 

> On Jan 27, 2023, at 1:10 PM, Reilly, Amie via Histonet 
>  wrote:
> 
> Good Morning,
> 
> I would like to post a question on histonet:
> 
> My organization is made up of multiple hospitals and surgery centers. All 
> specimens are couriered to the Pathology Department  which is located at only 
> one of the facilities. The sites without a fully operational pathology lab 
> provide frozen section support  and are equipped with grossing stations.  
> Currently any below and above knee leg amputations are held at the collecting 
> hospital and a pathologist is deployed to gross the leg at that location. 
> Only the blocks are transported to the pathology department. My question is 
> where do other systems with  multiple hospitals and surgery centers, with an 
> offsite pathology department, gross their amputations?  Is the entire limb 
> being transported to the offsite Pathology Lab or do personnel gross at 
> collecting location ? If the entire limb is being couriered, how is it 
> packaged?
> 
> 
> Amie Reilly, MLS (ASCP)CM
> Pathology Manager
> Catholic Health System
> Buffalo, NY 14221
> (Phone) 716-828-2440
> (Fax)  716-828-2791
> 
> 
> From: histonet-requ...@lists.utsouthwestern.edu 
> [mailto:histonet-requ...@lists.utsouthwestern.edu]
> Sent: Friday, January 27, 2023 1:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 230, Issue 13
> 
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Re: [Histonet] Dragon

[Cristi Rigazio via Histonet]

I used it exclusively in GI lab for years and loved it but we only did small 
biopsies, so it was super simple.  We use it now throughout the system I work 
in and there are a variety of opinions.  Some of the PAs love it and some 
really struggle.  Same with the pathologists.  So we live in dual worlds with 
both Dragon and MModal.  If you would like to contact me separately I am happy 
to discuss!

Thanks
Cristi

> On Feb 9, 2021, at 4:56 PM, Amanda Coscetti via Histonet 
>  wrote:
> 
> Does anyone use Dragon while grossing? We are looking at switching to Dragon 
> and wanted some feedback for use in pathology.  
> Currently, we use M*Modal for dictations and then have transcriptionists type 
> all of the information in Cerner reports. 
> 
> Thanks!! 
> Amanda 
> 
> 
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Re: [Histonet] Poll question

[Cristi Rigazio via Histonet]

Do you mean between specimens or per shift?

Thanks
Cristi

Sent from my iPhone

> On Feb 26, 2020, at 5:24 PM, Charles Riley via Histonet 
>  wrote:
> 
> How often does everyone clean their water-bath?
> 
> -- 
> 
> Charles Riley BS  HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
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[Histonet] Benchmarks for Reprocessing

[Cristi Rigazio via Histonet]

> Good afternoon Fellow histonetters!
> 
> I was wondering if there are any tertiary institutions out there that have 
> set a benchmark for reprocessing tissue?  We tend to be less than 1%, but 
> would love to see what others think is reasonable.
> 
> Thanks for the feedback and hope you all are having an excellent day!
> Cristi

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[Histonet] Benchmarks for Reprocessing

[Cristi Rigazio via Histonet]

Good afternoon Fellow histonetters!

I was wondering if there are any tertiary institutions out there that have
set a benchmark for reprocessing tissue?  We tend to be less than 1%, but
would love to see what others think is reasonable.

Thanks for the feedback and hope you all are having an excellent day!
Cristi
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Re: [Histonet] Breast lumpectomy fixation

[Cristi Rigazio via Histonet]

PAs and pathologists are the only ones that gross them, we have six hours of 
formalin on the processing schedule to ensure we meet the minimum fixation time 
and Saturdays are rotated to ensure we never exceed 72 hours.

Sent from my iPhone

> On Dec 19, 2018, at 6:38 PM, Bob Richmond via Histonet 
>  wrote:
> 
> Charles Riley BS  HT, HTL(ASCP)CM, Histopathology Coordinator/ Mohs
> (where?) asks:
> 
>>> How does everyone gross and process your breast lumpectomy / mastectomy
> specimens?<<
> 
> These are extremely complex and exacting procedures. It's your
> pathologist's job to do this, and your pathologist can't delegate any of
> the task to you without giving you a great deal of instruction. Don't try
> to charge into this unaided!
> 
> Bob Richmond
> Samurai Pathologist, ret.
> Maryville TN
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[Histonet] Beaker, Sendouts and ICD10 codes

[Cristi Rigazio via Histonet]

Hi histo land!

Anyone out there in the admin area using Beaker that can discuss workflows?  I 
am curious how others are handling the new OPPS Medicare rule for molecular 
testing?  Also, who is providing the ICD codes for your sendouts?

Hope everyone is having a great day!
Cristi

Sent from my iPhone
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Re: [Histonet] AFB STAIN

[Cristi Rigazio via Histonet]

We always try to put them in the same slide, but sometimes it doesn’t work out 
the way.  Either would be acceptable.

Sent from my iPhone

> On Oct 4, 2018, at 12:35 AM, Tony Henwood (SCHN) via Histonet 
>  wrote:
> 
> We do,
> 
> It works well
> 
> 
> Regards 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney 
> Tel: 612 9845 3306 
> Fax: 612 9845 3318 
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> -Original Message-
> From: ADESUPO ADESUYI via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Thursday, 4 October 2018 12:54 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] AFB STAIN
> 
> Hello,
> I have a question please. For the AFB Stain, do we put both the 
> control tissue and the patient on the same slide?
> 
> Thanks,
> Ade
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Re: [Histonet] Hazy/cooked cells in H

[Cristi Rigazio via Histonet]

Have you checked the processors?  It is easy to “mix up” the reagents.  
Has tissue collection changed? Are the specimens left out longer or placed on 
paper? 
 Do you use sponges?  Any chance the fixation time has been decreased?

Sent from my iPhone

> On Jul 18, 2018, at 3:46 PM, Mac Donald, Jennifer via Histonet 
>  wrote:
> 
> Is anyone using freezing spray?  We had this problem and all cases were 
> traced back to the same microtomist. She used the freezing spray excessively 
> and was causing “freezer burn” to the GI biopsies.
> Jennifer
> 
> Get Outlook for iOS
> 
> 
> From: Charles Riley via Histonet 
> Sent: Wednesday, July 18, 2018 11:12 AM
> To: Histo List
> Subject: [Histonet] Hazy/cooked cells in H
> 
> Our pathologists have said that we have had a few cases the past few days
> that are unreadable. They say the cells appear "cooked".
> 
> Nothing in our process has changed, we have been using the same lot of
> reagents since the middle of June, and no errors were reported in our
> processing runs.
> 
> Do anyone have any ideas to what could be causing this issue?
> 
> 
> --
> 
> Charles Riley BS HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
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[Histonet] Automated Embedder

[Cristi Rigazio via Histonet]

 Good morning fellow histo peeps!

We have been looking into the automated embedding system. The cutting room
is excited about this opportunity, but the grossing personnel have some
concerns. Is  anyone out there currently using this?  Can anyone provide
some input on tissue types, implementation, time differences in the use of
the cassette inserts, etc.,?

thanks,
Cristi
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Re: [Histonet] Histopathology text recommendations

[Cristi Rigazio via Histonet]

Ada Feldman does a great course in fundamentals!  I believe I have the power 
point from NSH last year if I can find it!

Sent from my iPhone

> On May 19, 2018, at 5:17 PM, Saundra Ellis via Histonet 
>  wrote:
> 
> Anything written by Freida Carson.
> 
> Saundra Ellis
> Histology Supervisor
> Florida Woman Care Laboratory
> Cell (360) 513-9665 (best contact)
> FAX (813) 433-5542
> 
> saundra.el...@floridawomancare.com
> 
> 
> 
> From: Gordon Brown via Histonet 
> Sent: Saturday, May 19, 2018 2:49:45 PM
> To: histonet@lists.utsouthwestern.edu
> Cc: Gordon Brown
> Subject: [Histonet] Histopathology text recommendations
> 
> Can anyone give me recommendations for a good histopathology text and atlas? 
> I'm not a professional or even a formal student, I'm an amateur microscopist 
> with a keen interest in histology, although I did attend a UK medical school 
> many years ago only to decide that medicine was not for me. I'm now retired 
> and expanding my interest in microscopy, which has fascinated me since the 
> age of 8, I now have a collection of decent microscopes and microtomes and 
> I'm setting up my study as a home microscopy and slide making facility. I 
> have a growing collection of old histology slides including a number of 
> pathology examples and I'm keen to be able to identify the histological 
> changes that take place due to disease. In particular I am looking to be able 
> to identify and photograph carcinomas as I recently acquired a 
> photomicrography bench used by the Imperial Cancer Research Campaign in 
> London during the 1950s and 1960s, and it would be appropriate to use it for 
> the purpose it was designed for.
> 
> Hope someone can help, I've subscribed to this list for many years but only 
> rarely posted anything, the last time was when I enquired about replaceable 
> blade holders for the original Cambridge rocking microtome and a very kind 
> histologist in the USA sent me a slightly damaged holder and some blades. 
> Fortunately I never managed to adapt it for the Cambridge as it is a perfect 
> fit for the Reichert and Leitz rotary microtomes I now own!
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[Histonet] Digital path

[Cristi Rigazio via Histonet]

Hi histoland,

I am exploring digital pathology and I am unclear about the current CAP 
requirements and how those may change if the platform is FDA approved for 
primary diagnosis.  Is the wash out period still necessary if it’s not 
considered and LDT?  Does the personnel still have to be qualified as high 
complexity testing personnel to scan the slides?  Any help or other information 
is greatly appreciated!

Thanks,
Cristi

Sent from my iPhone
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Re: [Histonet] Beaker with or without Vantage

[Cristi Rigazio via Histonet]

We use Beaker across our system and like anything it has its pros and cons.  
Tim is correct, it was NOT developed for Pathology, rather for physicians and 
the lab module was an afterthought.  Our IHC lab uses Vantage with Middleware 
and things go really well for them to my knowledge, but it certainly wasn’t 
without its challenges.

Changing any system is going to come with growing pains, for sure, but some of 
the things I would want “redone” include tracking, as a multiple site system we 
have issues with packing lists moving tests with materials to other labs and 
keeping the pathologists from signing cases out.  We lose our tracking from the 
gross station and pick it back up at embedding, using manual batch tracking 
sheets for what goes on the processors.  I believe something has been built to 
help with this and we use a wingman system, so others might not have this 
issue.  From a manager perspective, The report system is getting a little 
insane as with upgrades there seems to be unforeseen changes that impact 
reports you may rely on, also there are a variety of ways to develop or run 
reports (workbench reports, Clarity and Universe!) and the redundancy is 
getting confusing.  From the bench perspective, depending on the type of lab 
you are, accessioning is accomplished three different ways for us, client 
specimens that we have to register, within system specimens that are ordered in 
a manner that we have to go “grab” the order and the ideal way where we simply 
scan the label and input the task protocol (again within the system, but why 
can the doctors/nurses use two different ways to input the order??).  Scanning 
the barcodes from the cassettes can be an issue, but that is not Beakers fault, 
make sure you have solid cassette printers.  Lastly, the physicians view in 
Epic is much different than your view in Beaker, this has caused confusion when 
trying to find things like additional testing reports performed outside and 
then scanned in.  We have hammered through the majority of these issues and the 
Beaker team is great at helping out and thinking outside the box to smooth out 
workflows.  Oh, and billing!  Make sure you test all the way through patient 
accounting!  Stay positive, as noted, likely the choice isn’t yours to make, 
but how you approach it is.  Build a good relationship with your Beaker team, 
prioritize your needs and chip away.  It will work out.  

Sent from my iPhone

> On Nov 29, 2017, at 2:19 PM, Morken, Timothy via Histonet 
>  wrote:
> 
> Ginny, when you say "slow down our whole process" is that slowness in the 
> system or mismatch of the LIS to the pathology process?
> 
> My understanding is that this was originally designed for use in public 
> health labs (EpicLab), which are quite different than pathology labs. Now 
> they are trying to adapt a thirty-year old non-pathology system to pathology. 
> Sure would be nice if someone would just use a new pathology-specific LIS 
> built with new technology!  Why do we keep having to keep having 
> non-pathology people shove things on us and demand we adapt to their 
> ignorance? We went thru this with Copath for many years and had to teach them 
> how pathology works (they should have been paying us for our knowledge, not 
> charging us to teach them!). Now it seems it is coming around again in a new 
> form.
> 
> 
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
> 
> From: Kurth Virginia L. [mailto:vku...@uwhealth.org]
> Sent: Wednesday, November 29, 2017 10:16 AM
> To: Morken, Timothy; Martha Ward-Pathology
> Subject: Re: Beaker with or without Vantage
> 
> 
> Hello
> 
> We switched to beaker this year, and it definitely had growing pains.
> 
> I personally believe it was made for a doctor not a lab, and seemed to slow 
> down our whole process,
> 
> but everyone is going to it.  It helps patient care in the aspect of everyone 
> is on the same page with that
> 
> patient.  I think that more and more kinks will be worked out as it continues 
> to adapt to the lab setting.  I think
> 
> asking people when they switched should be considered because it has evolved. 
>  Good Luck!
> 
> 
> 
> 
> 
> Ginny Kurth
> 
> 
> UW Hospital of Wisconsin
> 
> 
> From: Martha Ward-Pathology via Histonet 
> >
> Sent: Tuesday, November 28, 2017 12:30:57 PM
> To: Morken, Timothy
> Cc: 
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Beaker with or without Vantage
> 
> WARNING: This email appears to have originated outside of the UW Health email 
> system.
> DO NOT CLICK on links or attachments unless you recognize the sender and know 
> the content is safe.
> 
> 
> 
> 
> "They" aren't the ones that will have to 

[Histonet] Fixation and IHC

[Cristi Rigazio via Histonet]

Hi histonetters!

Can anyone tell me what, if any, guidelines there are for fixation time for
animal tissue with potential subsequent IHC stains.  I am very familiar
with CAP guidelines for receptor testing with breast cases, but I can't
seem to find much on IHC for animal tissue.

Thanks,
Cristi
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Re: [Histonet] Send-Outs

[Cristi Rigazio via Histonet]

Thanks everyone for the responses!  And just to make sure...I was joking with 
the Amazon reference, lol!  But I do aim high!

Sent from my iPhone

> On May 16, 2017, at 2:37 PM, Dragoo, Michelle <michelle.dra...@nm.org> wrote:
> 
> We are the same as Tim. Typically we try to get things out within 24 hours, 
> that said, it depends on how old the case is, does it need recuts etc. etc. I 
> think your current time of 48 hours sounds quite reasonable. 
> 
> Michelle R. Dragoo, MBA, HT (ASCP)
> Supervisor, Histology 
> Northwestern Medicine Central Dupage Hospital
> 25 North Winfield Road, Winfield IL 60190
> O: 630.933.6748
> michelle.dra...@nm.org
> 
> 
> 
> 
> 
> -Original Message-
> From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] 
> Sent: Tuesday, May 16, 2017 10:53 AM
> To: Cristi Rigazio
> Cc: Histonet
> Subject: Re: [Histonet] Send-Outs
> 
> Really? You want to compare your lab to Amazon? If you have stock on-hand, 
> located in one place, have robots to retrieve it, people who do nothing but 
> pack boxes and trucks standing by to take any order at any time of day, then 
> you are in the league of Amazon and can receive an order and send it out 
> within an hour.
> 
> However most of us first need to find the material to send out - slides? 
> where are they? Pathologist, resident? waiting to be filed? Removed from file 
> by someone else? Can't find anything in THAT office...Same with blocks - not 
> in file, is there a card telling who took it? Maybe for recuts, maybe for 
> research, then back into the batch to be filed. Look 5 different places. 
> Frozen tissue is usually easier but, whoops...already sent some out for 
> another requested test...
> 
> Anyway, that is what my experience is!
> 
> We don't have any set time requirement. It could be an hour or a few days 
> depending on if can easily find the material they want to send. Usually it is 
> within a day.
> 
> 
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
> Pathology UC San Francisco Medical Center
> 
> 
> 
> -Original Message-
> From: Cristi Rigazio via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Monday, May 15, 2017 10:55 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Send-Outs
> 
> Hi Histoland!
> 
> We have been reviewing our send out procedures and I have been tasked with 
> researching what TAT's other institutions define.  Currently, we allow 48 
> hours for the preparation of "orders" to the time sent out.  Is this 
> reasonable?  Do other facilities allow more or less time?
> 
> I feel like Amazon can get something to my door step in a matter of hours, so 
> I must be missing something obvious in our steps that could be simplified, 
> but I can't put my finger on it.  Any feedback on what others are doing is 
> greatly appreciated.
> 
> Sincerely,
> Cristi
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[Histonet] Send-Outs

[Cristi Rigazio via Histonet]

Hi Histoland!

We have been reviewing our send out procedures and I have been tasked with
researching what TAT's other institutions define.  Currently, we allow 48
hours for the preparation of "orders" to the time sent out.  Is this
reasonable?  Do other facilities allow more or less time?

I feel like Amazon can get something to my door step in a matter of hours,
so I must be missing something obvious in our steps that could be
simplified, but I can't put my finger on it.  Any feedback on what others
are doing is greatly appreciated.

Sincerely,
Cristi
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[Histonet] ANP.22780 and validation

[Cristi Rigazio via Histonet]

Hi Histoworld and Happy New Year!

I am currently validating new processing schedules and am a little concerned 
about my understanding.  NSH guidelines don't say special stains, IHCs etc., 
need to be re validated when validating a new processor, schedules etc., but 
this CAP standard seems to imply it is required.  This, in turn, doesn't make 
sense to me as many labs send their blocks or unstained slides for subsequent 
testing to other labs.  Those labs don't know the processing schedule, 
equipment, etc.,  Can someone shed some light on this for me?

Thank you,
Cristi

Sent from my iPhone
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[Histonet] Peloris Processing Schedules

[Cristi Rigazio via Histonet]

Hi Histoland,

I am currently establishing new processing schedules for the Peloris
processor and wondered if anyone had a schedule for large tissue they would
be willing to share?  Also, maybe one for excisional/lumpectomy/mastectomy
cases?  I have been testing one for large tissue that is approximately 4
hours, but I think a little longer in formalin may be necessary.  Anyone
willing to share their experiences with the Peloris is greatly appreciated.

Happy Holidays,
Cristi
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[Histonet] Cold Ischemic time

[Cristi Rigazio via Histonet]

Hi histo land!

Referencing CAP requirement ANP.22983, how do you all document cold
ischemic time?  Do you have a "macro" that the pathologists input or a
disclaimer?  We were recently inspected and we include a "disclaimer" in
the reports that the time is less than one hour, but the inspector said
that it was not "Accurately documented in the report"...I want to fix this
this, of course but wondered if you all had any guidance such that I don't
try and recreate the wheel.  Thank you for your time!

Cristi
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Re: [Histonet] GI Biopsies

[Cristi Rigazio]

Three levels one slide for all biopsies.  Colon polyps one section.

Sent from my iPhone

On Mar 13, 2014, at 11:29 AM, imhype...@aol.com wrote:

 
 Good afternoon all,
 I was just curious about how your institutions handle GI biopsies, 
 specifically how many slides you cut off the bat.  We presently cut 2 levels 
 on each GI biopsy block, but I'm hearing that more and more places only cut 1 
 slide per GI biopsy block.  Please share what you are doing at your 
 establishment.
Thank you 
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Re: [Histonet] Rush bx schedule?

[Cristi Rigazio]

We do strictly GI biopsies, but if we have a stat case, we have a processing 
schedule as follows: five minutes in each station of one 70%, two 95%, two 100% 
and three xylenes, then 10 minutes in each of three paraffins.  We have the 
ASP300 and this has been very successful.  I have heard from others they do as 
little as 2 minutes in each, With a bit longer in the paraffin.  Generally our 
STAT cases are in formalin overnight so we skip that step altogether.
Happy Holidays!
Cristi

Sent from my iPhone

On Dec 4, 2013, at 9:31 AM, Morken, Timothy timothy.mor...@ucsfmedctr.org 
wrote:

 For tissue, what step most contributes to subsequent swelling of tissue when 
 the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin 
 infiltration all contribute, but at what percentage?
 
 Here is the current schedule, which apparently was meant to mimic a microwave 
 schedule that was used before the MW quit working. The tissue - kidney and 
 liver bx for the most part, up to 10 per day, is coming out soft and swells a 
 bit when soaked. So sectioning is difficult.
 
 We need to have a rapid schedule but need good sections. Obviously we are 
 pushing the limits on this so need a bit of leeway for variation in tissue
 
 So, I'm considering which of these steps is the most critical to lengthen.
 This is on a VIP5 but we can also use a peloris for this processing
 
 Formalin 2 min, 50degC (this is not necessary at all, IMHO)
 80% ETOH 5 min, 50degC
 95%ETOH 5 min, 50degC
 100% ETOH 8 min, 50degC
 Xylene, 9 min 50degC
 Paraffin, 4 min,
 Paraffin, 5 min
 
 Thanks for your insight!
 
 
 
 Tim Morken
 Supervisor, Electron Microscopy and Neuromuscular Special Studies
 UC San Francisco Medical Center
 Box 1656
 505 Parnassus Ave
 San Francisco, CA 94143
 USA
 
 415.353.1266  (office)
 tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org
 
 
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Re: [Histonet] Yahoo link

[Cristi Rigazio]

Thank you for sharing that!  Yikes!

Sent from my iPhone

On Dec 3, 2013, at 6:31 AM, Mike Tighe mti...@trudeauinstitute.org wrote:

 Anybody wonder who has the most harzardous job to your health? We're Number 
 One!!!
 
 
 
 http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html
 
 
 
 Mike
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Re: [Histonet] IHC lab who can do Veterinary Markers?

[Cristi Rigazio]

MPI does as well.  Melissa Martinek at 269-668-3336 ext 1271 can get you where 
you need to be.

Sent from my iPhone

On Nov 27, 2013, at 8:45 AM, Candice Smoots candice_cami...@yahoo.com wrote:

 You should try Idexx Laboratories!
 
 
 I remain yours truely, 
 
 Candice Camille 
 
 
 
 From: Rob Day r...@foliobio.com
 To: histonet@lists.utsouthwestern.edu 
 Sent: Friday, November 22, 2013 4:58 PM
 Subject: [Histonet] IHC lab who can do Veterinary Markers?
 
 
 You might try www.phylogenyinc.com.
 
 
 Looking  for a lab that can do IHC on veterinary tissue of all types,
   rodents(murine  and  rats),  canine, feline, equine, avia as many as a
   zoo  would  have..  Slides  would  be  precut,  usually with a control
   already  on  the  slide.  Lookng  for  a  lab  with  high quality IHC,
   expeditious  TAT  and  a  competitive cost. Would prefer DC/MD/VA area
   with  a  courier,  but  mailings  will  do  provided  it  is  not cost
   prohibitive.  For  those  who  are serious, please send information to
   [1]joseph.n.mad...@health.mil
 
 
   Very respectfully,
 
 
   Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
 
 References
 
   1. mailto:joseph.n.mad...@health.mil
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Re: [Histonet] The Good Old Days...

[Cristi Rigazio]

Hear hear!  I agree and was just saying I love hearing the stories (although I 
am not young).  Thanks all for sharing these memories and lessons!

Sent from my iPhone

On Sep 20, 2013, at 10:13 AM, Davis, Cassie cda...@che-east.org wrote:

 I enjoy hearing sincere reminiscing...Even though us kids don't know how 
 good we have it, some of us enjoy having an old tech beside us on the 
 bench. I find weeding through the sarcasm can be profitable and in doing so 
 have learned so much. What the old techs did on a daily basis, we only did in 
 the practice lab and when the automated instruments and pre-made solutions 
 that we have come to rely on fail, experience is so very valuable. Only by 
 their blood, sweat and tears have we benefitted however, we have so far to 
 go, let's do it together.
 
 Cassandra Davis
 cda...@che-east.org
 302-575-8095
 
 
 
 
 
 Confidentiality Notice:
 This e-mail, including any attachments is the
 property of Catholic Health East and is intended
 for the sole use of the intended recipient(s). 
 It may contain information that is privileged and
 confidential.  Any unauthorized review, use,
 disclosure, or distribution is prohibited. If you are
 not the intended recipient, please delete this message, and
 reply to the sender regarding the error in a separate email.
 
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Re: [Histonet] Handouts - NSH Symposium

[Cristi Rigazio]

Thank you Peggy!

Sent from my iPhone

On Sep 18, 2013, at 7:30 AM, Lee  Peggy Wenk lpw...@sbcglobal.net wrote:

 Giving everyone going to the NSH Symposium a heads up, in case they missed 
 reading the NSH email about handouts.
 
 NSH will NOT be providing printed out handouts for the workshops. They are 
 going green.
 
 NSH sent out an email around Sept. 10 (that’s when I received the email), 
 that had a subject line of “download your handouts”. If you want handouts for 
 the workshops you signed up for, the email directed you to a link, in which 
 you put in your email address and a password they provided you. You then go 
 to your workshops/lectures that you signed up for, and print off the PDF’s of 
 the handouts.
 
 I’ve talked with a couple of people recently, who didn’t realize this, so I 
 thought getting this information out another way through Histonet might be 
 helpful to those attending.
 
 Peggy A. Wenk, HTL(ASCP)SLS
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Re: [Histonet] RE: Histology Video

[Cristi Rigazio]

If one is organized for NSH, count me in!!

Sent from my iPhone

On Jul 18, 2013, at 11:09 AM, Bernice Frederick b-freder...@northwestern.edu 
wrote:

 In Providence?
 
 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 ECOGPCO-RL
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edu
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, 
 Karen - SMMC-SF
 Sent: Thursday, July 18, 2013 12:00 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Histology Video
 
 Does anyone know of any Histology parody videos out there on YouTube?  Just 
 curious!
 
 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org
   
Caution:  This email message, including all content and attachments, is 
 CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
 information contained in this email message is intended only for the use of 
 the recipient(s) named above. If the reader of this message is not the 
 intended recipient or an agent responsible for delivering it to the intended 
 recipient, you have received this document in error.  Any further review, 
 dissemination, distribution, or copying of this message is strictly 
 prohibited.  If you have received this communication in error, please notify 
 us  immediately by reply email.  Thank you.
 
 
 
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Re: [Histonet] Formalin Neutralizer

[Cristi Rigazio]

We neutralize ours and have no problems with it.  I am not sure how much you 
use, so I will say it is easiest in smaller batches as you do have to shake it 
up to make sure it dissolves.  We purchase ours from BBC Biochemical for a very 
reasonable price and then test before dumping.  We have doing this for four 
years with no issues.
Thanks,
Cristi

Sent from my iPhone

On Apr 11, 2013, at 6:04 AM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote:

 We are looking into the option of neutralizing our formalin waste instead of 
 having it to be picked up.
 If you use Formalin Neutralizer, do you have any  pro/con about this product?
 Thank you very much.
 
 Lin S. Bustamante, B.S., H.T.(ASCP)
 VIBS Histology Laboratory Supervisor
 College Of Veterinary Medicine
 Texas AM University
 College Station, Texas 77843-4458
 Phone: (979) 845-3177
 Fax: (979) 458-3499
 
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Re: [Histonet] Formalin Neutralizer

[Cristi Rigazio]

We have a formaldehyde test kit.  It's a dip stick type test.

Sent from my iPhone

On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote:

 Can I ask how you test before dumping?
 
 Thanks
 
 Mark
 
 On Apr 11, 2013 6:21 AM, Cristi Rigazio cls71...@gmail.com wrote:
 We neutralize ours and have no problems with it.  I am not sure how much you 
 use, so I will say it is easiest in smaller batches as you do have to shake 
 it up to make sure it dissolves.  We purchase ours from BBC Biochemical for 
 a very reasonable price and then test before dumping.  We have doing this 
 for four years with no issues.
 Thanks,
 Cristi
 
 Sent from my iPhone
 
 On Apr 11, 2013, at 6:04 AM, Bustamante, Lin lbustama...@cvm.tamu.edu 
 wrote:
 
  We are looking into the option of neutralizing our formalin waste instead 
  of having it to be picked up.
  If you use Formalin Neutralizer, do you have any  pro/con about this 
  product?
  Thank you very much.
 
  Lin S. Bustamante, B.S., H.T.(ASCP)
  VIBS Histology Laboratory Supervisor
  College Of Veterinary Medicine
  Texas AM University
  College Station, Texas 77843-4458
  Phone: (979) 845-3177
  Fax: (979) 458-3499
 
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Re: [Histonet] RE: AFB and negative control

[Cristi Rigazio]

Isn't a gram stain the only special that requires a positive and negative 
control?  

Sent from my iPhone

On Mar 14, 2013, at 1:51 PM, joelle weaver joellewea...@hotmail.com wrote:

 This practice is listed as a QC measure for issues of cross contamination in 
 the ASCP publication Quality Management in Anatomic Pathology, Nakhleh, R. 
 M.D. I have never had any issues that were persistant enough to warrant this 
 measure myself, but it is one of the suggestions made under the section for 
 use of control tissue/slides. 
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
 From: tnma...@mdanderson.org
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 14 Mar 2013 17:52:39 +
 Subject: [Histonet] RE: AFB and negative control
 
 While I don't use a negative control for the AFB, I will use distilled water 
 throughout the procedure.  Most of the time the water in the waterbath is 
 distilled as well, to rule out contamination there as well.  Make sure the 
 waterbath has been disinfected.
 
 Toysha N. Mayer, MBA, HT (ASCP)
 Instructor, Education Coordinator
 Program in Histotechnology
 School of Health Professions
 MD Anderson Cancer Center
 (713) 563-3481
 tnma...@mdanderson.org
 
 
 
 
 Date: Thu, 14 Mar 2013 12:42:30 +
 From: Ian R Bernard ibern...@uab.edu
 Subject: [Histonet] AFB and Negative Control
 To: Lee  Peggy Wenk lpw...@sbcglobal.net, Tighe, Sean T
sti...@ufl.edu,histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
 Message-ID:
d4f4c602b10b9f45b4e9271af6380e16181a1...@uabexmb1.ad.uab.edu
 Content-Type: text/plain; charset=utf-8
 
 The only special stain that I know that requires the use of a negative 
 control is for the AFB. I understand to rule out false positives as the AFB 
 bacteria might exist in tap water. Nevertheless, a good QA practice which we 
 will implement now.  
 
 Other than Carson, does anyone know of a regulatory or accreditation agency 
 is requiring this as well?  Any suggestion on a good control tissue type? 
 Carson recommends uterus.  Also if there is a pick up on the negative slide 
 (link to the tap water) will use of distilled water and a repeat procedure 
 fix this?
 
 Any thoughts from fellow histonetters?
 
 Thanks
 Ian Bernard
 
 
 
 
 
 
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Re: [Histonet] RE: Pregnancy

[Cristi Rigazio]

I told my doctor everything I dealt with on a daily basis and he said it was no 
problem.  I worked in a 400 sq ft space where we do everything including 
grossing!  My son is perfect, but had I known about everything you all are 
saying I would have done things differently!  Nothing is worth risking your 
little one.  If I am lucky enough to have another I want to thank you all for 
the input as I will not do it again!!

Sent from my iPhone

On Mar 5, 2013, at 3:20 PM, Elizabeth Chlipala l...@premierlab.com wrote:

 Karen
 
 Are you sure it was workman's comp.  Workman's comp applies to individuals 
 injured on the job.
 
 Liz
 
 Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
 Laboratory Manager
 Premier Laboratory, LLC
 PO Box 18592
 Boulder, CO 80308
 Work (303) 682-3949
 Fax (303) 682-9060
 Cell (303) 881-0763
 l...@premierlab.com
 www.premierlab.com
 
 Ship to address:
 
 1567 Skyway Drive, Unit E
 Longmont, CO 80504
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, 
 Karen - SMMC-SF
 Sent: Tuesday, March 05, 2013 11:55 AM
 To: 'Parker, Helayne'; histonet@lists.utsouthwestern.edu
 Subject: [Histonet] RE: Pregnancy
 
 I was working in a Histology Lab when I first got pregnant.  I told my doctor 
 about everything I was exposed to in the lab.  He actually opted on putting 
 me on medical leave (workman's comp).  I did this with both of my 
 pregnancies.  Not in less you can find her a job that she is not exposed.  I 
 would not risk exposure.
 
 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org
   
Caution:  This email message, including all content and attachments, is 
 CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
 information contained in this email message is intended only for the use of 
 the recipient(s) named above. If the reader of this message is not the 
 intended recipient or an agent responsible for delivering it to the intended 
 recipient, you have received this document in error.  Any further review, 
 dissemination, distribution, or copying of this message is strictly 
 prohibited.  If you have received this communication in error, please notify 
 us  immediately by reply email.  Thank you.
 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, 
 Helayne
 Sent: Tuesday, March 05, 2013 10:10 AM
 To: 'histonet@lists.utsouthwestern.edu'
 Subject: [Histonet] Pregnancy
 
 Hi Gang,
   Thought I would ask some people with some more experience than I in this.  
 One of our techs is pregnant-  Yay !!!  She is very early - about 5 weeks.  
 My Lab Director wants her to start wearing a mask due to the chemicals.  So 
 we need to ask what sort of precautions need to take place during the 
 pregnancy etc.  We also coverslip by hand here out of xylene etc.  I can not 
 remember ever working with any pregnant HTs in the past so I honestly do not 
 know.  Please carbon copy replies to jascholefi...@skaggs.net as well.  We 
 predominately work with 10% formalin, reagent grade alcohols, paraplast, 
 xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some 
 routine special stain items. Basically the typical myriad of stuff a very 
 small routine histo lab would have (no immunos)
 
 Thanks and bunch for your help !
 
 
 Sincerely,
 
 Helayne Parker, H.T. (ASCP)
 Pathology Section Head
 Cox Medical Center Branson
 P.O. Box 650, Branson, MO 65615
 Phone:  417-335-7254
 Fax:  417-335-7127
 Email:  hpar...@skaggs.net
 Web:  www.coxhealth.com/branson
 
 
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Re: [Histonet] Cassette labeling

[Cristi Rigazio]

We print out a procedure list that has the number of pieces on it and it next 
to the embedding center for reference while performing that task.  The only we 
write on the cassette is the accession number.
Thanks,
Cristi

Sent from my iPhone

On Feb 8, 2013, at 4:45 AM, SimmsColon, Janine janine.simmsco...@jmmc.com 
wrote:

 Good morning all, 
 
 
 
 I hope all here in the East are prepared for the storm, (considering I
 am in New England and it is February it is not all that surprising to me
 yet I am always amazed at the commotion that occurs with every impending
 snow, but I digress). I know many out there may have cassette labelers
 so this may not be an issue but for those who still label by hand I am
 curious as to how you label. At my previous lab besides the case number
 (S13-accession number), we also indicated how to embed the pieces (= for
 on edge), and the number of pieces in the cassette. I am just curious as
 to how common this practice is or if there is any other way you annotate
 the number of pieces expected in a cassette. Thank you in advance for
 any feedback you provide as I have received many helpful responses to my
 previous posts.
 
 
 
 Janine Simms Colon, BHA, CPhT, HT(ASCP)
 
 Histology/Pathology
 
 Johnson Memorial Hospital
 
 201 Chestnut Hill Road
 
 Stafford Springs, CT 06076
 
 Office: 860-684-8230 ext. 5197
 
 janine.simmsco...@jmmc.com
 
 
 
 
 The information contained in this message may be privileged and confidential 
 and protected from disclosure. 
 If the reader of this message is not the intended recipient, or an employee 
 or agent responsible for delivering 
 this message to the intended recipient, you are hereby notified that any 
 dissemination, distribution or 
 copying of this communication is strictly prohibited. If you have received 
 this communication in error, please 
 notify us immediately by replying to the message and deleting it from your 
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Re: [Histonet] Voice Recognition Systems

[Cristi Rigazio]

We use Dragon Speak, our LIS is WindoPath.
Cristi

Sent from my iPhone

On Jan 15, 2013, at 3:37 PM, Ann Specian thisis...@aol.com wrote:

 
 I am looking for a voice recognition system for the gross room.  It needs to 
 be a system that will interface with our LIS. 
 
 Does anyone have any recommendations in regard to vendors?
 
 Thank you,
 Ann Specian
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Re: [Histonet] Uneven Staining

[Cristi Rigazio]

As most other bases have been covered, do you leave space between the slides in 
the rack?  Once in a blue moon we had stains from the same rack that differed 
in intensity, after much troubleshooting we started putting space between the 
slides and we haven't had the problem since.  Just an idea.
Cristi

Sent from my iPhone

On Nov 27, 2012, at 12:24 PM, Rene J Buesa rjbu...@yahoo.com wrote:

 This is quite a puzzling situation.
 You have ruled out dewaxing (that was my first idea of cause) but I do not 
 think that soaking will be the problem because the sections are fixed and 
 infiltrated with paraffin, so there is no possibility of diluting in water.
 Thick/thin sections in a series is a very rare occurrence so I am also at a 
 loss with this problem. Weak staining solutions could cause it but all 
 sections in a series will be equally weak.
 Just in case, since you have covered the dewaxing, issue do the following: do 
 not leave the sections too long in the water bath and dry them in the oven as 
 soon as they are drained.
 René J.
 
 From: Elizabeth Cameron elizabeth.came...@jax.org
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
 Sent: Tuesday, November 27, 2012 1:35 PM
 Subject: [Histonet] Uneven Staining
 
 About a year ago, I posted about issues with staining mouse kidney sections 
 (see below for original post).  We realized that part of the problem was the 
 sections drying during staining, which has been resolved, but we are still 
 having some issues with some slides staining well and others being very pale. 
  This time it seems to be consistent across the slide, so I know it is not a 
 drying issue.  They are serial sections, so they are all cut together and 
 stained together, and they are from the same animal.  I don't believe 
 deparaffinization is an issue - we have tried longer times with fresh 
 solutions and it doesn't make a difference.  We have also tried re-staining 
 the slides after letting them sit in xylene overnight, and there is no 
 difference in the staining.  I am now thinking that it may be related to how 
 long the sections soak or how long they sit on the waterbath.  They are NBF 
 fixed, and there is a fine line between soaking well for
 hydration and oversoaking, resulting in swelling.  Has anyone experienced 
 anything like this?  Any other ideas?  I am at a loss, and I'd really like 
 for this to work!
 Thanks,
 Liz
 
 We are doing a Hale's colloidal iron stain (no counterstain) on serial 
 sections of mouse kidneys.  We are staining an entire kidney at a time (about 
 90-150 slides), and after many successful runs, we are now finding some 
 slides in each batch with very uneven staining.  Half of a section will stain 
 as it should, and the rest of the section is very pale.  It seems to be in a 
 similar area from one section to the next, but not exactly the same area.  It 
 does not look like a deparaffinization issue.  There may be two or three 
 slides in a row like this, or just one section on a slide, followed by 30-40 
 that look fine.  The sections are 6 microns. Any ideas on why this might be 
 happening?  Thanks!
 
 
 
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Re: [Histonet] Stain for HP

[Cristi Rigazio]

We do a Diff Quik on all gastric biopsies.

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On Oct 17, 2012, at 11:38 AM, Elaine Pettigrew cpett...@gmail.com wrote:

 We do warthin-starry  on all stomach biopsy,and than IHC if Dr. Request
 
 On Wednesday, October 17, 2012, ihcman2...@hotmail.com wrote:
 
 IHC on BOND autostainer.  Automatic on gastrics
 
 Glen Dawson.
 
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 - Reply message -
 From: Diana McCaig dmcc...@ckha.on.ca javascript:;
 Date: Wed, Oct 17, 2012 10:03 am
 Subject: [Histonet] Stain for HP
 To: Histonet histonet@lists.utsouthwestern.edu javascript:;
 
 What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
 Do you do them routinely or only when requested?
 Are they done on an autostainer or with a kit?
 
 Diana
 
 
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Re: [Histonet] Why am i getting zoosk invites?

[Cristi Rigazio]

Is that what that is?  I got it too! 

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On Oct 12, 2012, at 7:46 PM, Erinandjesseconley erinandjessecon...@yahoo.com 
wrote:

 
 I have no interest in a dating service. Why would histonet be affiliates with 
 this site?
 
 
 
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Re: [Histonet] fo a small GI lab.... Question

[Cristi Rigazio]

We have a metal drum, 55 gallon that is  stored at rm temp.  We have a weekly 
check that we document.

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On Jul 14, 2012, at 4:14 PM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote:

 How do you store your waste before been picked up by any company. I need to 
 know what is acceptable by CAP Inspectors; temperature for the room. Our 
 containers are outside lab, etc. Any information will be very much 
 appreciated.
 Thank you.
 Lin Bustamante
 
 
 
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