Re: [Histonet] [External] Superfrost adhesion problems?

[Cynthia Robinson via Histonet]

We have seen surface tension issues with staining on slides. We had several 
lots and were able to remove the bad lots from our stock and that has helped.


Cindi Robinson HT(ASCP)

Histology Lead

MercyOne Siouxland Medical Center

Dunes Medical Laboratories

101 Tower Road, Suite 220

Dakota Dunes SD 57049


From: Fulton Regan via Histonet 
Sent: Tuesday, December 3, 2019 4:39 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [External] [Histonet] Superfrost adhesion problems?

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

Histonetters,

We have heard some anecdotal reports of a recent decrease in the adhesive 
performance of Superfrost slides, with tissue falling off during IHC staining 
on automated instruments.  We use a lot of these slides and we may have 
experienced this problem.  Has anyone else encountered this problem recently?

Thanks.


Regan Fulton, M.D., Ph.D.

Array Science, LLC
475 Gate 5 Road, #102
Sausalito, CA 94965
www.arrayscience.com




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Re: [Histonet] Marking Tissues with Eosin

[Cynthia Robinson via Histonet]

We use safranin at the grossing station and it is a dark pink at embedding. 
Works really well in our hands. Added plus is no fluorescent issues that you 
can have with eosin.

Cindi


From: Cindy Bird via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, June 23, 2016 4:20 PM
To: Mca Werdler
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Marking Tissues with Eosin

We place a small drop of concentrate straight on tissue.

Sent from my iPhone

> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet 
>  wrote:
>
> Dear Rebecca,
>
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
>
> Good luck,
>
> Maarten
>
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet@lists.utsouthwestern.edu>:
>
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block.  I've heard mention of marking these with
>> eosin to make them easier to see.  Has anyone done this?  Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>>
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>>
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Re: [Histonet] Frozen Section WL and Billing

[Cynthia Robinson via Histonet]

Aren't fs CPT codes 88331 and 88332?


From: Terri Braud via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, August 28, 2015 1:05 PM
To: histonet@lists.utsouthwestern.edu
Cc: Cassandra P. Davis
Subject: Re: [Histonet] Frozen Section WL and Billing

We set up our system this way -
Example:
A specimen for frozen is sent.  You cut 2 blocks of frozen tissue, 2 levels ea.
The PA/Pathologist submits 3 additional blocks for a total of 5 blocks on the 
specimen (1-2 are the previously frozen, 3-5 additional tissue) to be cut at 
one level each.
1. At accession, the technical bill for the Lev 4 gross and micro (88305) drop 
based on the specimen type (skin biopsy)
2. The tech enters a protocol for 5 blocks, 1 HE stain each block.
3. The tech modifies the stains, and changes the HE on the first block to a 
stain  called FS1 (Frozen Section, first block) The charge for the first frozen 
block, 88341, drops in when this stain is ordered
4. The tech modifies the stains, and changes the HE on the second block to a 
stain called FSA (Frozen Section, Additional Block)  The charge for the 
additional frozen block, 88342, drops in when this stain is ordered.
5.  The tech then enters stains that are set up in the stain dictionary as a 
Label Only. There is no charge associated with these stains.
Stain FL1,  Block 1,
Stain FL2,  Block 1,
Stain FL1,  Block 2,
Stain FL2,  Block 2,
6. FL1 = Frozen Label, 1st level
   FL2 = Frozen Label, 2nd level
7. You can define as many of these Frozen Label levels as you will ever need.
8. Print the labels for the case (some systems will allow you to set up a print 
job for by Label Type, and you can select Labels Only)

It sounds a bit complicated to set up, but the steps are simple, and once it 
has been set up to use this way, it is quick, easy and accurate to use.  I 
don't know what LIS system you are using, but this is easily adaptable for 
almost any system
We also set up the system to include the Label Only slides to be included in 
the total slide count, so you get work credit there.
I hope this helps.
Please feel free to call or contact for any questions.
Also, I'm not that far from you, if you need a little help.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
   2. frozen section- workload and billing (Davis, Cassie)
From: Davis, Cassie cda...@che-east.org
To: histonet@lists.utsouthwestern.edu

Hi Histofolks,
   I need to pick yours brains...we are in the middle of building a 
workable computer system for our lab we have run into a hiccup when it come to 
frozen sections. As a tech I know there is actual hands on, stop what you are 
doing, do this now  work involved. My understanding from a billing perspective 
it is not billable workload but an interdepartmental consultation between 
surgery and pathology. The problem is how to build the system so we get labels 
for our frozen section slides that does not interfere with the billable 
workload that is. I was thinking maybe it should be built in the system the 
same way a control slide is, does anybody have any suggestions?

Cassandra Davis
Histology Technician
Anatomical Pathology Laboratory
Saint Francis Healthcare
701 N. Clayton Street
Wilmington,DE 19805
Office:  302-575-8095
Email:  cda...@che-east.orgmailto:n...@che-east.org
www.saintfrancishealthcare.org



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[Histonet] RE: (no subject)

[Cynthia Robinson]

A few years ago we got cited for not having the fungal control be in tissue. 
The citation was from a HQIP survey we participated in from CAP. We were using 
a cultured fungal specimen in a cell button at that time. Since then I have 
collected and shared a number of cases we have seen with fungal infections in 
feet. 

Just my 2 cents

Cindi Robinson, HT(ASCP)
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Mayer,Toysha N 
[tnma...@mdanderson.org]
Sent: Tuesday, April 21, 2015 2:12 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: (no subject)

-I think you can use the other tissue controls.
 Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls 
free of charge, and I think they are armadillo.
The best control I have ever used for mast cells is canine mast cell tumors.  I 
request them from Vet Schools regularly.
Also think of antibodies, aren't  most antibodies animal?
The separation of the specimens I think is during processing.  They should be 
processed separately from routine human tissues when they are being used for 
clinical human tests.
I have heard of them running on the same processor, just on a different run 
when the solutions have been changed.

Toysha
-

Message: 10
Date: Mon, 20 Apr 2015 18:50:15 -0400
From: Garrey Faller garr...@gmail.com
Subject: Re: [Histonet] (no subject)
To: koelli...@comcast.net
Cc: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
CAF2sxrVD7o96Wz84nDBR6uxf=93tpqpmueqwog57e4fetcc...@mail.gmail.com
Content-Type: text/plain; charset=UTF-8

Here is the CAP checklist requirement:
ANP.21450
All  histochemical stains are of adequate quality, and daily controls are
demonstrated on each day of use for the tissue components or organism for
which they were designed.

Ray...you should call the CAP and ask for guidance on this.
My interpretation of this requirement is that it should be OK to use a
fungus from an orange peel. An orange peel fungus should have the same
staining characteristics as a candida or aspergillus etc.  Similarly a
bacteria is a bacteria. If you can produce a control that has both gram
positives and negatives, it should be OK. But, don't quote me on this.

Call the CAP for a definitive answer. I am interested in their response.
Garrey

On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote:

 I asked about this in a different vein months ago.  Has anyone shown a
 strawberry or ground meat or slim jim or orange peel as a bacteria/fungus
 control used for diagnostics to an inspector inspecting the lab and was
 there any comment from the inspector either positive or negative. Never
 heard back anything.
 Ray, Lake Forest Park, WA

 - Original Message -

 From: tjfinney2...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Sent: Sunday, April 19, 2015 5:24:53 PM
 Subject: [Histonet] (no subject)

 GMS controls
 From my understanding we can't use non human controls on patients. I
 could be wrong, but you may want to look into it.

 Happy Connecting.  Sent from my Sprint Phone.

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--


Sent from my iPhone

 On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.com wrote:

 Here is the CAP checklist requirement:
 ANP.21450
 All  histochemical stains are of adequate quality, and daily controls are
 demonstrated on each day of use for the tissue components or organism for
 which they were designed.

 Ray...you should call the CAP and ask for guidance on this.
 My interpretation of this requirement is that it should be OK to use a
 fungus from an orange peel. An orange peel fungus should have the same
 staining characteristics as a candida or aspergillus etc.  Similarly a
 bacteria is a bacteria. If you can produce a control that has both gram
 positives and negatives, it should be OK. But, don't quote me on this.

 Call the CAP for a definitive answer. I am interested in their response.
 Garrey

 On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote:

 I asked about this in a different vein months ago.  Has anyone shown a
 strawberry or ground meat or slim jim or orange peel as a bacteria/fungus
 control used for diagnostics to an inspector inspecting the lab and was
 there any comment from the inspector either positive or negative. Never
 heard back anything.
 Ray, Lake Forest Park, WA

 - Original Message -

 From: tjfinney2...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Sent: Sunday, 

[Histonet] Leica Aperio

[Cynthia Robinson]

Does anyone have experience setting up a report template for a breast panel on 
the Aperio? We just got ours set up and haven't had training yet. The thing has 
been here since November but with holidays and schedules not working it has 
been a trial. Are trying to get a jumpstart before the pathologists start 
training and think it should be ready to go immediately.



Thanks.



Cindi Robinson HT(ASCP)

Mercy Medical Center-Sioux City

Dunes Medical Laboratories

350 W Anchor Dr

Dakota Dunes SD 57049

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RE: [Histonet] I need opinions on Leica instruments

[Cynthia Robinson]

We have the Peloris II and VIP processors, as well as Ventana.We are happy with 
the Peloris. It processes fatty tissue very well. The versatility with 2 
retorts in one machine has allowed us to have several different processing 
times based on tissue size. We did have a learning curve with the Peloris as we 
have a smaller volume, 200 blocks per day. Initially we had to be more on top 
of the reagents and learn when to change versus waiting for the machine to 
signal a change. I wish Leica would have been a bit more helpful and up front 
about this. Leica did tell us that might have some issues due to our use of 
mesh cassettes and volume. I would be happy to answer questions  if you would 
like to contact me directly.


Cindi Robinson, HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Drive
Dakota Dunes SD 57049



 Duddey, Aimee adud...@firsthealth.org 4/24/2014 6:28 AM 
I am considering the Peloris II tissue processor and the Bond IHC stainer for 
our lab.  We are a 400 bed regional hospital with a growing outreach business.  
We have had Thermo Excelsior processor for years and have never really been 
happy with it.  Of course our VIP is as reliable and consistent as they come.  
We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special 
stainer).  I would appreciate any feedback about Leica's line of products.  
Please include experiences good and bad.
Thank you in advance.

Aimee M. Duddey, MLT(ASCP)
Assistant Director of Laboratory - Pathology FirstHealth Moore Regional 
Hospital Pinehurst, NC 28374
(910) 715-5286
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[Histonet] RE: Instrument Verification

[Cynthia Robinson]

I agree with you in that CAP is just looking for things to change and doesn't 
seem to be considering the change and decrease in staffing seen in clinical 
settings. Cryostat validation? Reallycut a slide after you have cleaned and 
pm'd the thing and go on. Good grief...I don't need any more paper and 
documentation on routine processes. As for tissue processors, I have 20 year 
old VIP's that have been running and producing specimens acceptably. I did 
validate them prior to being put in use but we didn't document like we do now. 
And I don't see the need to do it at this stage of use. We did do a very 
extensive validation on the Peloris we put into use last year and will going 
forward on new equipment.  To me the daily QC of stain should provide our 
'validation' of the process and include the processor. I am interested in 
others thoughts as well.

Thanks for allowing me to rant.

Cindi Robinson, HT(ASCP)
Mercy Medical Center-Sioux City
Dunes Medical Laboratories
350 W Anchor Drive
Dakota Dunes SD 57049




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Thursday, April 03, 2014 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Instrument Verification

I just received my midcycle CAP and for cryostat validation, we are planning to 
cut and stain a piece of frozen tonsil and have the path sign off on it. For 
the tissue processors, we will run a one minute test program. I hope this will 
fly.  Is it just me, or is CAP insanely out of control with new or modified 
regulations and policies for AP?  

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

6. Validation of cryostat (Gloria Tharp)

Message: 6
Date: Thu, 3 Apr 2014 09:59:26 -0500
From: Gloria Tharp gth...@pcasoutheast.com Could anyone tell me how you are 
handling the new CAP ANP.23045 question on function and verification of 
equipment regarding a cryostat.
Gloria Tharp, BA, HTL(ASCP)
--

Message: 7
Date: Thu, 3 Apr 2014 15:26:17 +
From: Leann M. Murphy lmurp...@aultman.com How is everyone validating the 
tissue processor for new CAP ANP.23045 question on function and verification of 
equipment?
LeAnn Murphy
Aultman Hospital
Canton, Ohio

-



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[Histonet] Hep Par 1 validation

[Cynthia Robinson]

I am wondering if anyone has hepatoblastomaeither in liver or metastatic 
tumorin their tissue bank. I am working up Hep Par 1 and have a few cases 
but would like a few more. We are working up this antibody to use in metastatic 
case with unknown primary as a way to rule out possibilities. My pathologist 
would really like to see a case with tumor surrounded by normal liver, if 
possible. If you can help please contact me. I have control blocks I would be 
willing to trade.

Thanks.Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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Re: [Histonet] (no subject)

[Cynthia Robinson]

If you use anything higher than 70% alcohol after the 10% buffered formalin you 
will have salt precipitate out and cause all kinds of problems. We use 70%, 
80%, 90% alcohol in the stations after the formalin and have not seen any 
issues with our Peloris or the VIPs. 


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Tony Auge tony.a...@gmail.com 07/15/2013 12:47 
I recently was having salt percipitation problems with my VIP proccesor and
we were doing a hot water flush every week. We were starting with 90%
alcohol and heat on the first station and also using 10% NBF. We switched
to 50% in the first station and 70% in the second station. This has not
only got rid of the salt problem but our small tissue biopsies look much
better. I'm not sure what your technicians concerns are about but from my
experience lowering the concentration of the first alcohols is more gentle
for the smaller tissues but it might not processes big fatty specimens as
well. There will be also more reagent carryover from the water introduced
in the first station but is worth it in my opinion. Good Luck!


Tony Auge HTL QIHC (ASCP)
Cell: (651) 373-4768
Email: tony.a...@gmail.com 
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[Histonet] urine hemosiderin

[Cynthia Robinson]

What kind of slide preparation are you using for urine? Cytospin vs thin prep 
vial is the discussion we are having. And are you doing a manual or automated 
iron stain? Thanks for any info you can provide.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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[Histonet] PC control

[Cynthia Robinson]

I am in need of pneumocystis control. Does anyone have any to share? I have a 
supply of fungus controls I can trade.

Thanks..Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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[Histonet] AFB control

[Cynthia Robinson]

All,

Does anyone have AFB control in human tissue they would be willing to share? I 
shared fungal controls awhile back with anyone who emailed methat is, until 
I ran out. I would be willing to share control material if I have something you 
need.

Thanks.


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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[Histonet] RE: Changing dynamics in histotechnology

[Cynthia Robinson]

I worked as a generalist for 4 yrs and then was in Microbiology for 8 yrs prior 
to moving to Histology in 1990. I have always felt the experience of working in 
the other areas of lab helped me understand the entire process, especially 
those involving fluids which were shared between multiple disciplines within 
clinical laboratory. We have an MT school and many of those students ask to 
spend time in Histology to observe. I appreciate their interest and willingness 
to learn how AP and Clinical Lab fit together. I have promoted the field by 
encouraging tours with local high school students taking Anatomy, Advanced 
Chemistry and Advanced Biology classes. 
Several of these students are now working as MTs, CTs and HTs. I now have a 
daughter interested in Histology because of the many trips to the lab to visit 
me. She will be moving to the KC area and will be completing her AS there. I 
just hope she can find a hospital willing to teach her Histology as there are 
no schools in the area. If anyone has a contact or would be willing to mentor 
please let me know. 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson
Sent: Monday, September 17, 2012 5:07 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Re: Changing dynamics in histotechnology

Ok, my workplace blocks Facebook, so here is the article for those of you who 
can't read it from the original link provided: 
http://www.clpmag.com/issues/articles/2012-09_04.asp 

Many good points have already been raised and discussed and I will not rehash 
them here. Here are my thoughts on the matter:

- First - kudos for the NSH, state societies, committee members and histology 
professionals for working their butts off to provide us with information and 
training opportunities, and for promoting our profession. They are doing what 
they can to provide the water for us to drink. It is up to us to partake in it.

- Why are we keeping this information in laboratory-centric publications? How 
in the world are we ever going to get the word out about our shortages and 
challenges unless we move outside of our own little box? Advance, Laboratory 
Medicine, NSH, etc are only read by personnel currently involved in laboratory 
testing. Sorry but we've been talking about this for YEARS and almost always in 
Lab publications. Is anything happening? What about People Magazine, or USA 
Today, or Sunday Morning or Good Morning America?

- We have long fought to keep Med Techs from coming into the histology lab and 
taking over the higher complexity testing because they have a 4-year degree and 
most of us don't. To say that it is a mistake to bring them in because only 
histologists fully understand the preparation process and its effects of the 
variation of results and can effectively work, partner with the pathologist to 
provide the information and testing results required to make personalized 
medicine a reality is like trying to hide behind a shield made of aluminum 
foil. If we can learn it on the job (as most of us have), then so can they. 
Encroachment by MTs might be the single biggest factor in promoting education 
in our field.

- I'm wondering if anyone(in clinical laboratory education) has started 
thinking about putting a histology component into Med Tech training. I know 
their schools are in trouble as well, but maybe the answer isn't to stay 
separate but to consolidate? I know, some of you are howling right now because 
this is an emotional issue for us. But take a moment to consider that other 
countries require folks who do Histology to be biomedical scientists, 
proficient in many laboratory disciplines including Histology. If we cannot 
adapt and educate ourselves with or without the assistance of the NSH, local 
Histo groups, pathologist support or employer support then I consider this may 
be a potential answer to the staffing issues.

- Having said all this - I like being separate from Med Techs. I like what 
makes us different. We make a decent wage considering the current lack of 
formal education requirement. I'm often surprised our profession doesn't make 
the list of higher paying jobs without advanced degree requirement. I am 
thinking that it's probably a good thing it hasn't as it might inadvertently 
promote the status quo.

Teri Johnson HT(ASCP),QIHC

Disclaimer: The thoughts conveyed above are strictly my own and do not reflect 
in any way on my employer, co-workers, family members, deceased pets, and 
future ex-husbands.

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Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 

[Histonet] Job opportunity

[Cynthia Robinson]

238 bed acute care hospital with affiliated reference laboratory in the MidWest 
is currently looking for a histotechnician or histotechnologist (ASCP 
registered or equivalent preferred).  Responsibilities will include, but are 
not limited to, specimen processing of surgical and cytology specimens, 
cutting, embedding, and both basic H  E stains as well as special stains, IHC, 
ISH stains.  Works closely with the pathologist while grossing and performing 
frozen sections.  
Please apply at our web-site 
http://www.healthcaresource.com/mercysiouxcity_i/index.cfm?fuseaction=search.categoryListtemplate=dsp_job_categories.cfm
 or call Pat Cable (712-233-5243) for more information.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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Re: [Histonet] Eosin staining for small biopsies

[Cynthia Robinson]

We use safranin (used in Microbiology as a counterstain)  on our small 
biopsies. We apply a small drop during grossing. It does not affect staining of 
HE, IHC or ISH. We like this because it is an intense red that doesn't leach 
out in the alcohols of processor.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Freeman, Carol carol.free...@utoledo.edu 6/20/2012 8:46 AM 


Good Morning all, Happy Wednesday!

I have a question and I am not finding much on my google search...First
does anyone have any papers written or studies done on the use of Eosin
to stain small biopsies or the use of eosin on the tissue processor in
the last alcohol??  I have read snippets on eosin having an effect on
FISH testing?? Does anyone know of this to be true and have any papers
or studies to refer back to or any documentation showing this result??
One more thing does anyone use Hematoxylin to mark small tissue biopsies
and/or any thoughts on its use to do so?? My thoughts are that because
of it's precipitation qualities it could gunk up the tissue processor
and leave residue precipitate at embedding.. agree? Disagree?

Thanks for any responses.

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[Histonet] Fungus control

[Cynthia Robinson]

All,

We have a lung case that is loaded with fungus. Is anyone in need?



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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Re: [Histonet] mesocolon lymphnode treatment with aceton

[Cynthia Robinson]

I would also be interested if anyone is using this method and their results. I 
have printed off the article and shared the information with our supervising 
pathologist. I have found dermal needle rollers on line but wonder if the 
maximum 2 mm length will do the job of perforating the fat. Also would like 
more information on the compression device used. 

Thanks..Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Gudrun Lang gu.l...@gmx.at 3/7/2012 11:24 AM 
Dear all!

Does anyone out there use this method for mesocolon clearing with aceton? I
heard only a few facts about it and would like to hear about personal
experience.

The mesocolon is first fixed in NBF, then put in pure aceton, then treated
with a teasing roller and put in a pipe with holes. The tissue is
compressed to squeeze out the fat to form a kind of sausage.

The sausage is sliced up, the slices are put into cassettes and processed.

I don't know the exact details. If you have the access to this article, you
can read more (I don't have). 

http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_ 
in_Rectal_Cancer__UICC.5.aspx

or this:

http://www.springerlink.com/content/748617k331l06143/ 

 

thank you

 

Gudrun Lang

 

Biomed. Analytikerin

Histolab, Linz, Austria

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Re: [Histonet] Validation

[Cynthia Robinson]

Amber,

Optimizing the antibody is the first step of validation. Running the new 
protocol against previously stained cases is the second step and shows you are 
getting results as good as, if not better, with the new vs the old.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Amber McKenzie amber.mcken...@gastrodocs.net 11/16/2011 12:20 PM 

Just wondering what y'alls opinion is on validation:  I don't really understand 
why optimization isn't enough.  At that point, the pathologist has said what he 
wanted the stain to look like, so why do 3-10 positive slides on the new 
instrument to compare to previous slides from another instrument?  Thanks!

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RE: [Histonet] (no subject)

[Cynthia Robinson]

It is a good thing this vendor does not work in a histo lab because with that 
comment/attitude he/she would not last long. I have heard the water bath theory 
but upon investigation this has never been the source because every histotech I 
work with cleans it each and every time. We have traced floaters back to 
grossing stations, processors...especially placenta getting 'snagged' on a 
piece of bone...embedding forceps (we now use ones with no grooves), forcep 
warmers, stains and coverslippers. We have significantly reduced the number we 
see since we make the effort to track each one found as an ongoing QA project, 
but we still see the occasional floater.  We did find that we have to put 
tissue types which fragment easily into mesh cassettes or bags but this can 
cause issues with fluid exchange and carryover during processing. It is a 
balancing act. I have always wondering about the newer processors with 
orientation cassettes which are embed and then cut without opening. Do they see 
less floaters with this type of 'closed' system? 



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 CHRISTIE GOWAN christiego...@msn.com 10/6/2011 7:44 AM 

I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I 
have seen floaters come from a variety of places but I am hard pressed to 
remember any floater coming from the water bath. Today we are blessed with DNA 
fingerprinting to determine if the floater is or is not from the patient but 
that still does not address the real issue of where did it come from and how do 
we stop it. The vendors stating that it is not an issue have never been 
re-biopsied because of a floater in with their tissue. Good discussion and long 
overdue. I look forward to the day when it is no longer an automatic response 
from all involved that it is Histology's fault. Hope you are enjoying your 
new adventures in retirement. See you next year in Canada!
 

 From: mamaw...@hotmail.com 
 To: histonet@lists.utsouthwestern.edu 
 Date: Wed, 5 Oct 2011 14:11:25 -1000
 Subject: [Histonet] (no subject)
 
 
 Hello everyone,After being home from the NSH for a few weeks I have been 
 pondering an issue that I think bears discussion on the histonet.There have 
 been several papers published regarding floaters and the amount determined 
 to come from traditional staining buckets. There was also a poster presented 
 at the NSH this year on the subject.When I approached several vendors of HE 
 stainers about this issue. The answers were surprisingly pretty much the 
 same. It is not an issue! Now I understand how one company can make this 
 claim as their stainer uses fresh stain on each slide. The explanations from 
 the other companies were insulting and just plain did not make sense to me. I 
 was told by a Histo tech vendor that All Histo techs know that floaters come 
 from the water bath. Well, she was talking to a histo tech and I know for a 
 fact that floaters come from a variety of places. I have seen them from the 
 doctor's office or procedure room to the stainer and every step in between. 
 Sometimes if the floater is in the block it is very difficult to determine 
 where it originated. We can however eliminate the water bath and stainer as 
 the origin in these cases. One company told me that the design of the 
 solution bottle eliminated floaters because floaters float and their stainer 
 draws solutions from the bottom of the bottle. I have probably changed 
 thousands of staining dishes during my 40+ year career (yes, I am old) and I 
 have seen lots of little pieces of tissue at the bottom of the staining 
 dishes. So, no, not all floaters float. I would love to hear feedback from 
 others on this. I guess I would appreciate feedback about the floater issue 
 as well as how a few vendors can make such claims and expect Histology techs 
 to buy it. I really felt that a few comments were insulting to our profession 
 and to the knowledge and expertise we possess. JanOmaha 
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Re: [Histonet] Retirement

[Cynthia Robinson]

Bill,

I am about the same age as you are and would like the same type of locale. 
Since there are so many of us in this field due to retire in the next 15-18 
years maybe we should consider investing in a Histotech Retirement Community. 
Specimens could be shipped to us for processing and staining. We could set up 
microtome stations in the common area and we could get together and talk and 
cut at the same time and do it in a wonderful climate with nice views and of 
courseat our own pace which will be relaxed and more of a shuffle that a 
full out sprint. Anyone have any suggestions for naming such a paradise? 

Ok...I'm just getting old and it is Friday..so hope you appreciate my humor.

Have a good weekend everyone!
From here in the Midwest, where waterfront property is in abundance along the 
Mighty MO

Cindi

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 O'Donnell, Bill billodonn...@catholichealth.net 6/17/2011 11:00 AM 

 OK, I know it is Friday, and I know that this may sound like a bit of a
jokebut I am 15-18 years out from retirement and my wife and I
want to retire someplace tropical And it would be smart to get
settled in such a location. So, if anyone knows of any openings in
Hawaii, Virgin Islands, St. Thomas, Puerto Rico for an experienced HT
(ASCP) QIHC  PLEASE let me know. Would be open to others, but would
prefer a US territory. I can be reached at b...@deaconbill.com 

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist/Safety Officer
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.



 


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[Histonet] Her2neu testing in gastric cancer

[Cynthia Robinson]

We have oncologists ordering Her2neu on gastric cases since Herceptin has now 
been approved for this treatment. Is anyone using Ventana Pathway Her2neu 
antibody clone for gastric tumor? How did you do validation and what disclaimer 
are you using?  

Thanks for your help.


Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com



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RE: [Histonet] LIS, HIS question

[Cynthia Robinson]

We have Cerner Millennium and have been successful having nursing order in 
PowerChart and the order comes over into Pathnet. Some of the information 
required doesn't transmit currently. However, it is available to the path in 
PowerChart. You can contact me off line if you like, Jan. Also, I will be at 
the NHS meeting and I heard you are going as well. If so I could bring some 
examples of specimen labels and how we built the generic order in the Clin Lab 
system for tissues, fluids, pap, and bone marrows and we could have dinner 
together.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Feher, Stephen sfe...@cmc-nh.org 09/10/2010 10:24 AM 
What LIS and HIS systems are you using Jan?  We are beginning our quest
to do electronic order entry between SoftPath and our Sunrise HIS
system.  I know that Dartmouth Hitchcock Med Ctr in NH was successful in
doing this from Cerner Millennium as well. 


Steve

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Friday, September 10, 2010 8:39 AM
To: histo...@pathology.swmed.edu 
Subject: [Histonet] LIS, HIS question

Good Morning Everyone, Happy Friday,
I'm interested to know if anyone is paperless with Histology ordering
from surgery.  We still use requisitions and are beginning the quest to
go paperless but are having difficulty with all the variables we have in
regard to the specimens we receive.
Jan Mahoney
Omaha, NE
GO HUSKERS!



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RE: [Histonet] New CAP question ANP.22760

[Cynthia Robinson]

I do this but it has to be separate runs or wait until the kit is just running 
out. I also keep the slides from the initial QC run and compare it with the new 
lot to assure same intensity staining is obtained when changing lots.

Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 Mike Pence mpe...@grhs.net 6/18/2010 11:41 AM 

I don't think I can do this with the automated system we are currently
using. Ventana. Does any other Ventana users know if you can do this in
parallel

Mike
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen
Yee
Sent: Thursday, June 17, 2010 7:21 PM
To: Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu 
Subject: RE: [Histonet] New CAP question ANP.22760


Sorry, I should have included it.  
   
ANP.22760  Are new lots of antibody and detection system reagents tested
in parallel with old lots?  (NOTE: New lots of primary antibody and
detection system reagents must be compared to the previous lot using an
appropriate panel of control tissues.)  
   
Ellen Yee
  _  

  From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] 
To: Ellen Yee [mailto:e...@dpmginc.com] 
Sent: Thu, 17 Jun 2010 08:47:38 -0700
Subject: RE: [Histonet] New CAP question ANP.22760

Can you give us the wording of that question/checklist item? Laurie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ellen
Yee
Sent: Wednesday, June 16, 2010 10:10 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] New CAP question ANP.22760

How are IHC labs complying with this question? What is considered an
appropriate panel of control tissues? What do you stain to test your
detection systems? 

Ellen Yee 

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Re: [Histonet] ventana

[Cynthia Robinson]

We print a worksheet for each pathologist who will be reading slides
from the IHC run.  If we had to circulate one sheet to get every line
signed it would be problematic as we service two locations.  We just
were CAP inspected and it went great.



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com


 anita dudley azdud...@hotmail.com 04/27/2009 12:07 PM 

just wondering for those who have the benchmark, do you print out the
reports for every run and have the dr.s sign?  we were told the every
line on the form needs to be signed.  it is a lot of extra writing we
feel and are trying to come up with a qa sheet for immuno, special
stains and he all on one.  does anyone have any suggestions?  thanks

 

anita

providence hosp

mobile alabama

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[Histonet] bone marrow biopsies

[Cynthia Robinson]

We are currently using 10% formalin fixation on our bone marrow cores.  We fix 
for 2 hours minimum prior to decal.  We are using Immunocal from Decal Corp. 
for 2-4 hrs followed with processing overnight in VIP.  Cores are still crunchy 
upon sectioning and we are doing surface decal for up to 30 min. Our paths want 
cores turned out within 24 hrs following procedure. Any suggestions? 
 
Thanks.
Cindi 
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Re: [Histonet] Helicobacter

[Cynthia Robinson]

We keep track of our positive cases and only cut one ribbon for use as a
positive control.  This way we don't use up the block.
 
Cindi

 Rene J Buesa [EMAIL PROTECTED] 09/18/2008 10:51 AM 
The reason why the pathologists are usually reluctant to use (+) cases,
is because if they are used as a (+) controls they will be probably used
up totally within a short period of time and IF there is some sort of
legal action against them or the hospital in one of those cases there
will be no a block to return to and make additional sections for
litigation purposes, and it is an understandable position.
René J.

--- On Thu, 9/18/08, Matt Roark [EMAIL PROTECTED] wrote:

From: Matt Roark [EMAIL PROTECTED]
Subject: Re: [Histonet] Helicobacter
To: histonet@lists.utsouthwestern.edu 
Date: Thursday, September 18, 2008, 10:46 AM

Thanks everyone!  I also thought that we should be using positive
patient 
controls but our pathologist wasn't to keen on the idea for some
reason. 
But now with all of your responses maybe we will be.

Thanks again!

Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267


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