RE: [Histonet] Prostate Biopsy code--One more point

2015-03-05 Thread Daniel Hewitt
We were just having a debate about these charges, if I have 20 prostate
bx's can I only charge G0416 one time or 20 times?

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lester
Raff MD
Sent: Thursday, March 05, 2015 11:15 AM
To: Anita Buchiane; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Prostate Biopsy code--One more point

Also, as of 2015, the G0416 code is used for ANY NUMBER of prostate
biopsies for Medicare, not just 10-20.

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lester
Raff MD
Sent: Thursday, March 05, 2015 10:11 AM
To: Anita Buchiane; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Prostate Biopsy code

For commercial, each individually submitted prostate biopsy is coded as
88305.

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anita
Buchiane
Sent: Thursday, March 05, 2015 10:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Prostate Biopsy code

I know the CMS (Medicare) code for 10-20 prostate biopsy specimens is
G0416 but what is the Cpt (non-Medicare) code?



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RE: And other crazy stuff. RE: [Histonet] cutting honey bees

2015-01-08 Thread Daniel Hewitt
I have done a stink bug, spider and a few other creepy crawlers for my
kids to look at under the scope, they have no idea what they are looking
at but still love it.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Thursday, January 08, 2015 3:20 PM
To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg;
'histonet@lists.utsouthwestern.edu'
Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees

We did a goldfish once, interesting microscopically and difficult for
peeling (lots of keratin?)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/6/15, 12:23 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote:

You crazy research people...OK, so what is the craziest thing you ever
had to cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single
oocyte
that was nearly microscopic. I'll just say it took a LOT of thick
sections too face down to it without actually cutting through it.


Open the floodgates

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol
methacrylate (gma) it is much harder and would give better sections, we
have done zebra fish and several other harder tissues including
calcified
bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



 From: r...@psu.edu
 To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
 Date: Sat, 3 Jan 2015 23:15:33 +
 Subject: RE: [Histonet] cutting honey bees
 CC: 
 
 I sectioned and stained honey bee and yellow jacket stingers years
ago.
 They wanted to show the difference between the stingers.  I wasn't
sure
what to do so I processed and handled like everything else.  I was
able
to get some good sections.  I put 6 stingers in each block and cut
several sections figuring there should be at least one good stinger in
each block and it worked.
 Roberta Horner
 Penn State University
 Animal Diagnostic Lab
 
 From: histonet-boun...@lists.utsouthwestern.edu
 [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas
Gregg
 [classic...@gmail.com]
 Sent: Saturday, January 03, 2015 6:08 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] cutting honey bees
 
 Has anyone had experience embedding and cutting honey bees. I am sure
 there are some issues with the harder exoskeleton. Would that have to
 be dissected away first. I am considering helping a student with a
 science fair project on bees.
 
 Douglas Gregg
 Veterianary pathologist
 
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RE: [Histonet] Cell Block Preparation

2013-09-06 Thread Daniel Hewitt
We recently switched most of our cell blocks from agar to Histogel,
works great. We use small disposable embedding mold, put the specimen in
the bottom and add the histogel about half way up the mold, let it
harden, pop it out and put it in the cass. Also cuts much better than
agar.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Friday, September 06, 2013 7:18 AM
To: 'Tom McNemar'; 'Ann Specian'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation

I wonder if this method could be used with the product Histogel. Has
anyone tried it?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Friday, September 06, 2013 5:46 AM
To: 'Ann Specian'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation

This is how we do it now.  In the old days, we used agar and to my mind,
it is still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro)
- Pour the agar into the conical tube and spin for 5 minutes
- The agar will re-solidify and whatever sediment there is will be
concentrated in the very tip of the cone
- The agar will slide out of the centrifuge tube
- Slice off the very tip and wrap in lens paper
- Place the wrapped tip in a cassette and process as usual
- Embed the specimen tip down and you are good to go...

I still use this method today when I feel it necessary.  Works great.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann
Specian
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation


I am getting complaints in regard to insufficient cell blocks.  We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.

Does anyone have a more current technique which renders better
cellularity?

Also, do you know which renders a better cell block:  a fresh specimen,
a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
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RE: [Histonet] RE: p16 control tissue

2013-07-09 Thread Daniel Hewitt
We use a nice CIN3 on a cervical cone.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom
McNemar
Sent: Tuesday, July 09, 2013 1:59 PM
To: 'Laurie Colbert'; Histonet Post(histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: p16 control tissue

We are using Biocare's ab with squamous cell ca as the control of
choice.  I believe you can also use cervical dysplasia or tonsil.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
Sent: Tuesday, July 09, 2013 1:50 PM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] p16 control tissue

We are going to be running the p16 antibody from Biocare (RUO) on skin
specimens.  What type of tissue should I run for a positive control?
The data sheet says normal testis..

Laurie Colbert
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RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)

2013-03-21 Thread Daniel Hewitt
I agree a lot of baby sitting with the leica, our other lab has the new
Sakura which uses glass instead of tape, they love it.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Sullivan, Beatrice
Sent: Thursday, March 21, 2013 8:18 AM
To: Bitting, Angela K.; joelle weaver; Contact HistoCare;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)

I'd like to use something a little stronger to describe how much I
dislike this coverslipper.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting,
Angela K.
Sent: Thursday, March 21, 2013 8:04 AM
To: joelle weaver; Contact HistoCare; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)

We hate Leica's CV5030 coverslipper too.!!! Babysit is a good word to
describe the user experience.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
weaver
Sent: Wednesday, March 20, 2013 7:32 PM
To: Contact HistoCare; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)

I agree. The Leica stainer and Sakura are both good instruments, however
I really HATE that Leica glass cover slipper and I had the same
assessment as to need to baby sit. I sometimes preferred just hand
cover slipping because it was less trouble, and even faster sometimes (
believe that or not). I had none of these issues with the Prisma covcr
slipper, and no trouble with the user interface etc. I had the same
issue with the door latch on the Prisma, but other than a quick
replacement of that, it worked perfectly.




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: cont...@histocare.com
 Date: Wed, 20 Mar 2013 17:49:22 -0500
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)
 
 Both stainers are powerhouses. The Leica has a plain menu screen with
a simple interface while the Sakura has a LCD screen with detailed
information about what stage the staining process a rack is along with
multiple menus.  The difference between the performance changes
drastically when the respective coverslipper attachments become
involved.
 
 The Leica is seriously no match for the Sakura in this respect. The
Leica's coverslipper is its Achilles heel and requires a LOT more
attention and alerts frequently, very frequently. It takes a separate
rack for staining the slides at the beginning of the process and
eventually transfers them to a different rack one the cover slip is
complete. This one uses glass and frequently drops glass, creates
bubbles, drops and breaks slides. You will have to frequently purge the
system and clean the cover medium needle dropper.  Once done, it only
holds. Two racks of 30 slides and will alert until you remove it. You
can't leave this one alone for more than 5 minutes without an alert.
Seriously.
 
 The Sakura's coverslipper uses cover tape which won't need to be
replaced not even remotely as soon as the glass in the Leica.  Finished
slides remain in a carousel at the top and can hold about 10 racks of 20
before it alerts. For high volume, the Sakura pair wins hands down. You
won't lose productivity time by needing to check on this machine pair.
 
 HistoCare.com
 
 
 
 
 
 
 Hi,
 
 We are currently looking to switch out our linear MKII stainer for 
 either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any
recommendations?
 Are quantity of HEs is increasing and we need adequate equipment to 
 meet our workload. The incorporated oven seems excellent on both 
 stainers. Any pros/cons would be greatly appreciated! Also, if you are

 currently using the stainer, does it meet your workload and what is
your volume?
 
 Thanks!
 
 Sophia
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RE: [Histonet] Ultra

2012-11-26 Thread Daniel Hewitt
We have the same issue from time to time on our Ultra, our vortex mixers
seem to clog after about 3-4 months of use. The engineer comes in,
unclogs them and everything is good for another 3-4 months. They have
never really been able to figure it out why they clog but, but
thankfully the service is still covered, just a large nuisance.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce
Gapinski
Sent: Monday, November 26, 2012 1:50 PM
To: 'cthorn...@dahlchase.com'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Ultra

I too have had problems with my Ultra. I am so glad I place a control at
the top of every slide. Sometimes no Hematoxylin, sometimes no positive
staining. I've documented half a dozen cases.
One thing we noticed is that the antibody vials get
plugged up from the protein in the antibody. We inspect every vial
before we place it on the instrument. The other thing we look for is
antibody in the spout of the vial. It will recede. Called Ventana, and
they said  There are plenty of extra drops in the vial, so prime the
vial. Bad advise, here's why. The instrument has no idea how many drops
are disposed of during priming. So we ended up with another bunch of
primaries with no drops left. Now we prime without expelling any reagent
and how that works better.
With this problem I feel Ventana owes us URA (Ultimate
Reagent Access) then we can prime as we go.

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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RE: [Histonet] looking p16 - where is mtm labs?

2012-06-12 Thread Daniel Hewitt
P16 is now only sold through Ventana, believe they bought MTM or the
rights to P16.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Settembre, Dana
Sent: Tuesday, June 12, 2012 12:06 PM
To: Vickroy, Jim; histonet@lists.utsouthwestern.edu
Subject: [Histonet] looking p16 - where is mtm labs?

Looking to get current pricing and availability from mtm labs on p16 and
no one 
answers their phone - 800# nor their direct#
Seems Roche has taken them over.  
Need to reach them.
Any help would be appreciated
Dana Settembre
Immunohistochemistry
University Hospital - UMDNJ
Newark, NJ
USA

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RE: [Histonet] automatic stainer/coverslipper combos

2012-01-19 Thread DANIEL HEWITT
Sakura has come out with a new coverslipper which seems to work well, we
are trying it at one of our labs. In our lab we are currently using the
leica and all I can say is it has good days and really bad days, needs
lots of TLC.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Katelin
Lester
Sent: Thursday, January 19, 2012 1:59 PM
To: mequita praet
Cc: histonet
Subject: Re: [Histonet] automatic stainer/coverslipper combos

We have the Sakura Tissue-Tek Prisma with attached tape coverslipper and
I
love it. I had so many problems with the Leica (which can be attached to
it's coverslipper but mine wasn't) I would never go back. Feel free to
email me offline with any specific questions.
Katelin

-- 
Katelin Lester, HTL
Gastroenterology Specialists of Oregon, P.C.
Pathology Laboratory
(971) 224-2408


On Thu, Jan 19, 2012 at 10:29 AM, mequita praet mdpr...@gmail.com
wrote:

 Hi All,
 I am interested in your opinions of the automatic stainer/coverslipper
 combos. Do some companies still make them as one unit or just
compatible?
 Would you please give me an idea of which ones seem to work the best
and
 most reliably?
 Thank you,
 Mequita Praet
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RE: [Histonet] Keeping Histo room floor clean?

2011-06-15 Thread DANIEL HEWITT
We us a scraper I bought at Home Depot called the floor buster.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of CHRISTIE
GOWAN
Sent: Wednesday, June 15, 2011 10:21 AM
To: sgoe...@mirnarx.com; histot...@imagesbyhopper.com;
rosenfeld...@hotmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Keeping Histo room floor clean?


We use a razor blade scraper but we turn it around and use the dull
side.
 

 Date: Wed, 15 Jun 2011 08:59:21 -0500
 From: sgoe...@mirnarx.com
 To: histot...@imagesbyhopper.com; rosenfeld...@hotmail.com
 Subject: RE: [Histonet] Keeping Histo room floor clean?
 CC: histonet@lists.utsouthwestern.edu
 
 You can buy this type of thing too if you aren't the McGiver type. For
 instance...from American Mastertech item # CPW04200E
 
 Sarah Goebel-Dysart, BA, HT(ASCP)
 Histotechnologist
 Mirna Therapeutics
 2150 Woodward Street
 Suite 100
 Austin, Texas 78744
 (512)901-0900 ext. 6912
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 histot...@imagesbyhopper.com
 Sent: Tuesday, June 14, 2011 7:50 PM
 To: JR R
 Cc: histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Keeping Histo room floor clean?
 
 We don't keep it off the floor, but do use a wide-bladed putty knife
 attached to a mop handle to scrape the residual wax off the floor. It
 woks quite nicely and doesn't remove the actual floor wax like a razor
 blade scrapper would.
 
 Michelle
 
 Sent from my iPhone
 
 On Jun 14, 2011, at 6:44 PM, JR R rosenfeld...@hotmail.com wrote:
 
  
  We keep getting a lot of paraffin on the floor of one histo
 room--especially around the microtome and the embedding station.
  
  Short of laying down a tarp, what do folks do keep wax off of the
 floor?
  
  Thanks,
  
  Jerry Ricks
  Research Scientist
  University of Washington
  Department of Pathology
 
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RE: [Histonet] cryostat repair

2011-03-31 Thread DANIEL HEWITT
Emily
You can try Ray Brodersen at Brodersen Instrument Co, they are in Mars
and do most of our repairs. Ray does an excellent job at a good price.
It sounds like the bearings in your fan or maybe the compressor.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

This email, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
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responsible for delivering the message to the intended recipient, please
contact the sender by reply e-mail and delete and destroy all copies of
the original message, including attachments.

Please note that any views or opinions presented in this e-mail are
solely those of the author and do not necessarily represent those of
Heritage Valley Health System.  The integrity and security of this
message cannot be guaranteed on the internet.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Thursday, March 31, 2011 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cryostat repair

Our cryostat (CM3050S) is making a terrible noise when the fan stops
running, yet I don't want to call Leica (the manufacturer) because they
charge more money than anyone could ever afford.  How would I go about
finding someone who could repair a cryostat in Pittsburgh? I don't think
it's the refrigeration that's broken, it sounds like something
mechanical.
Any suggestions?
Has anyone ever tried to open the back of one to see if it's fixable?
I'm no
mechanic, but I think I can spot a worn belt or loose screw.  I'm just
not
sure I want to open it up myself.

Emily

It has become almost a cliche to remark that nobody boasts of ignorance
of
literature, but it is socially acceptable to boast ignorance of science
and
proudly claim incompetence in mathematics.
-Richard Dawkins
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