I have done a stink bug, spider and a few other creepy crawlers for my kids to look at under the scope, they have no idea what they are looking at but still love it.
Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Michael Ann Jones Sent: Thursday, January 08, 2015 3:20 PM To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg; '[email protected]' Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 [email protected] On 1/6/15, 12:23 PM, "Morken, Timothy" <[email protected]> wrote: >You crazy research people...OK, so what is the craziest thing you ever >had to cut, or were asked to cut? > >For me, not too bad, but embedding for EM and sectioning a single oocyte >that was nearly microscopic. I'll just say it took a LOT of thick >sections too face down to it without actually cutting through it. > > >Open the floodgates.... > >Tim Morken > >-----Original Message----- >From: [email protected] >[mailto:[email protected]] On Behalf Of Patsy >Ruegg >Sent: Tuesday, January 06, 2015 11:13 AM >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu >Subject: RE: [Histonet] cutting honey bees > >for the whole bee I probably would process and embed it in glycol >methacrylate (gma) it is much harder and would give better sections, we >have done zebra fish and several other harder tissues including calcified >bone in GMA. > >Cheers, >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >Ruegg IHC Consulting >40864 E Arkansas Ave >Bennett, CO 80102 >H 303-644-4538 >C 720-281-5406 >[email protected] > > > >> From: [email protected] >> To: [email protected]; [email protected] >> Date: Sat, 3 Jan 2015 23:15:33 +0000 >> Subject: RE: [Histonet] cutting honey bees >> CC: >> >> I sectioned and stained honey bee and yellow jacket stingers years ago. >> They wanted to show the difference between the stingers. I wasn't sure >>what to do so I processed and handled like everything else. I was able >>to get some good sections. I put 6 stingers in each block and cut >>several sections figuring there should be at least one good stinger in >>each block and it worked. >> Roberta Horner >> Penn State University >> Animal Diagnostic Lab >> ________________________________________ >> From: [email protected] >> [[email protected]] on behalf of Douglas Gregg >> [[email protected]] >> Sent: Saturday, January 03, 2015 6:08 PM >> To: [email protected] >> Subject: [Histonet] cutting honey bees >> >> Has anyone had experience embedding and cutting honey bees. I am sure >> there are some issues with the harder exoskeleton. Would that have to >> be dissected away first. I am considering helping a student with a >> science fair project on bees. >> >> Douglas Gregg >> Veterianary pathologist >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >[email protected] >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >[email protected] >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
