[Histonet] Dr. Salah Deeb ?

2015-05-19 Thread della Speranza, Vinnie
If there is anyone on this list who can provide contact information for Dr. 
Salah Deeb from Egypt, please contact me off list. 
Thank you,

Vinnie Della Speranza | Manager for Anatomic Pathology Services| Medical 
University of South Carolina | 165 Ashley Avenue MSC 908 | Charleston, South 
Carolina 29425 | Office: 843.792.6353 | Fax: 843.792.8974 | del...@musc.edu




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Re: [Histonet] Billing question

2015-05-08 Thread della Speranza, Vinnie
This is incorrect. The response from Joyce Weems is correct.

And yes, it's confusing to everyone.



Special stains are billed per block

IHC is billed per specimen



So a GMS on blocks A1 and A2 is GMS charge x 2



If you are not charging special stains per block you are losing revenue you are 
entitled to for 2015



Vinnie Della Speranza | Manager for Anatomic Pathology Services| Medical 
University of South Carolina | 165 Ashley Avenue MSC 908 | Charleston, South 
Carolina 29425 | Office: 843.792.6353 | Fax: 843.792.8974 | del...@musc.edu









-Original Message-
From: Campbell, Tasha M. [mailto:tmcam...@fmh.org]
Sent: Friday, May 08, 2015 7:51 AM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Billing question







From my understanding its just one charge.  Its per specimen such as A, B, C, 
etc.  Someone else said that it would be 2 charges so am I wrong on this??  If 
you did GMS on A1 and AFB on A2 then that would be 2 charges because its two 
different stains.









Tasha Campbell, B.S.,HTL(ASCP)

Frederick Gastroenterology Associates

310 W. 9th St.

Frederick, MD 21701

301-695-6800 ext. 144 (w)

304-685-9307 (c)



-Original Message-

From: Vickroy, James [mailto:jvick...@springfieldclinic.com]

Sent: Thursday, May 07, 2015 4:58 PM

To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu

Subject: [Histonet] Billing question



If I have two sections on an A specimen A1 and A2 and both had a GMS stain, 
do I charge 1 - 88312 or  2 - 88312?



Jim



Jim Vickroy

Histology Manager

Springfield Clinic, Main Campus, East Building

1025 South 6th Street

Springfield, Illinois  62703

Office:  217-528-7541, Ext. 15121

Email:  
jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com%3cmailto:jvick...@springfieldclinic.com







This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
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[Histonet] hands free scalpel blade system ?

2013-10-25 Thread della Speranza, Vinnie
I would appreciate hearing from anyone who is using a hands-free scalpel blade 
system that works well.



If you are not familiar with what I referring to, we are looking to a means of 
removing used scalpel blades from scalpel handles and installing new blades 
hands free, without the need to touch the blades.



We currently use the Bladex system but are searching for something that works 
more reliably.

If you know of an alternative to Bladex I would appreciate hearing from you.



Thank you,



Vinnie Della Speranza

Manager for Anatomic Pathology Services

Medical University of South Carolina

165 Ashley Ave. MSC 908

Charleston, SC 29425

Ph. 843-792-6353

Fax. 843-7928974




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[Histonet] RE: SOX-10

2012-10-08 Thread Della Speranza, Vinnie
Ronnie,
I've not started using this marker just yet (I've pre-ordered it for mid Oct 
availability), but I just received a product sheet for Cell Marque's polyclonal 
rabbit ab which is available in concentrate or RTU
If this interests you I can scan and email it to you (don't want to spam your 
inbox without your permission)

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue   MSC 908
Charleston, South Carolina 29425
tel. 843-792-6353
fax. 843-792-8974

CONFIDENTIALITY NOTICE
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and exempt from disclosure under applicable law.  If the reader of this message 
is not the intended recipient, you are hereby notified that any dissemination, 
distribution, or copying of this communication is strictly prohibited by law.  
If you have received this communication in error, please notify me and the MUSC 
Compliance Office immediately at (843) 792-4037 or 1-800-296-0269.  Thank you

From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of 
Houston, Ronald
Sent: Monday, October 08, 2012 12:24 PM
To: histonet@lists.utsouthwestern.edu; ih...@googlegroups.com
Subject: [IHCRG] SOX-10

Is anyone using SOX 10 in the diagnosis of soft tissue tumors? If so, can you 
please recommend a good reliable source for the antibody?

Thanks

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.orghttp://www.nationwidechildrens.org/

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[Histonet] RE: Barcoding specimen tracking, lessons you learned

2012-04-24 Thread Della Speranza, Vinnie
Hi Tim,

One thing I did not anticipate that was an unfortunate eye opener. 

When humans are given a scanner to read barcodes they seem to trust the 
technology so much that they stop reading with their eyes. 

We had an unfortunate incident where the wrong barcode label was placed onto a 
specimen container at accessioning. Keep in mind the label containing the 
barcode also contained a patient name and MRN. Had the next individual in the 
chain of events, the individual at the grossing table, read the label, she 
might have noticed the discrepancy. Instead, the barcode on the cassettes was 
for the wrong patient and of course, the slides likewise. 

So while I adopted barcodes to reduce the likelihood of error, this event made 
me feel a bit more vulnerable because once the scanners are in use, you may 
find that staff become so reliant on the technology that they are no longer 
vigilant in keeping an eye out for problems. I'm sharing this in the hope that 
by alerting your staff to this pitfall you can avoid experiencing this in your 
lab.


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, April 23, 2012 1:31 PM
To: Histonet
Subject: [Histonet] Barcoding specimen tracking, lessons you learned

To anyone who has implemented a barcoding/specimen tracking system in your lab. 
What lessons did you learn that would make it easier if you did it over? We're 
starting the process and I would like to get some input on things to look out 
for!

Thanks for any info and comments!

Tim Morken
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94132

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.mor...@ucsfmedctr.org


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RE: [Histonet] RE: Barcoding specimen tracking, lessons you learned

2012-04-24 Thread Della Speranza, Vinnie
Thanks for contacting me Kim. I think that staff feel compelled to be more 
vigilant when manual systems are in place and may get a bit too complacent, 
expecting the electronic tools to think for us. we are working on developing 
our own tools for scanning cassettes before they are loaded into the tissue 
processors, but the tool is in development stage and I can't offer specifics 
yet, however it is supposed to go live in July. We are creating a version of 
CoPath that will be married to Cerner Millenium. At the grossing table, the 
cassettes will be scanned when the tissues are collected and held in formalin, 
then scanned again at the tissue processor and again at the embedding station. 
At embedding, a screen pops up on a monitor that will alert the tech is there 
are special embedding instructions. This is entirely home grown and not an off 
the shelf product. I share this because the scenarios you mentioned are in fact 
possible, I lived through the nightmare of having to search through two tractor 
trailer loads of red bag waste to find a missing kidney biopsy. The specimen 
was found, but it was the most unpleasant experience imaginable. And I survived 
to tell about it ! 


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974


-Original Message-
From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] 
Sent: Tuesday, April 24, 2012 4:25 PM
To: Della Speranza, Vinnie
Cc: Morken, Timothy; Histonet
Subject: Re: [Histonet] RE: Barcoding specimen tracking, lessons you learned

Vinnie. I as well found some quirks with this technology and I'm glad you 
brought up that it isn't perfect. I had a bog issue with the fact that you 
couldn't scan all your cassettes when putting on the machine as I felt that was 
the time errors needed to be noticed and fixed. Because finding out even the 
next day that a specimen is missing is not a good time. Ie. Specimen got tossed 
into garbage upon grossing and garbage has been incinerated. Not that I've seen 
this. Just that it could happen. I also noticed that errors at microtomy can 
still happen. Think about this, you pick up a block. Scan it and then can't get 
a section. You put block back in decal or on ice. You pick up next block. 
Whamo. You better pay attention 
So in essence with what ever we use technology wise it's up to us if we are 
paying attention and should never be complacent with our tools. Because our 
tools still require us to think. 
Kim D

Sent from my iPhone

On Apr 24, 2012, at 2:25 PM, Della Speranza, Vinnie del...@musc.edu wrote:

 Hi Tim,
 
 One thing I did not anticipate that was an unfortunate eye opener. 
 
 When humans are given a scanner to read barcodes they seem to trust the 
 technology so much that they stop reading with their eyes. 
 
 We had an unfortunate incident where the wrong barcode label was placed onto 
 a specimen container at accessioning. Keep in mind the label containing the 
 barcode also contained a patient name and MRN. Had the next individual in the 
 chain of events, the individual at the grossing table, read the label, she 
 might have noticed the discrepancy. Instead, the barcode on the cassettes was 
 for the wrong patient and of course, the slides likewise. 
 
 So while I adopted barcodes to reduce the likelihood of error, this event 
 made me feel a bit more vulnerable because once the scanners are in use, you 
 may find that staff become so reliant on the technology that they are no 
 longer vigilant in keeping an eye out for problems. I'm sharing this in the 
 hope that by alerting your staff to this pitfall you can avoid experiencing 
 this in your lab.
 
 
 Vinnie Della Speranza, MS, HTL(ASCP)
 Manager for Anatomic Pathology Services
 Medical University of South Carolina
 165 Ashley Avenue MSC 908
 Charleston, SC 29425
 tel. 843-792-6353
 fax. 843-792-8974
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, 
 Timothy
 Sent: Monday, April 23, 2012 1:31 PM
 To: Histonet
 Subject: [Histonet] Barcoding specimen tracking, lessons you learned
 
 To anyone who has implemented a barcoding/specimen tracking system in your 
 lab. What lessons did you learn that would make it easier if you did it over? 
 We're starting the process and I would like to get some input on things to 
 look out for!
 
 Thanks for any info and comments!
 
 Tim Morken
 Department of Pathology
 UC San Francisco Medical Center
 505 Parnassus Ave, Box 1656
 Room S570
 San Francisco, CA 94132
 
 (415) 353-1266 (ph)
 (415) 514-3403 (fax)
 tim.mor...@ucsfmedctr.org
 
 
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RE: [Histonet] RE: Barcoding specimen tracking, lessons you learned

2012-04-24 Thread Della Speranza, Vinnie
 in order to protect the innocent I want to mention that the lost kidney 
biopsy that was successfully found in a mountain of red bag waste occurred at 
another facility and not where I am currently employed.  This is one of those 
experiences you never forget but fortunately had a happy ending and has been 
the subject of workshops I've given for NSH.


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974


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[Histonet] Vendors who market slide and cassette labelers - Can you help?

2012-02-01 Thread Della Speranza, Vinnie
 I am working on a project for NSH and CAP and I thought that the list might 
help me to avoid overlooking vendors that I may be unaware of. I am trying to 
identify ALL sources of cassette and slide labeling equipment. In order avoid 
clogging the list I invite you or vendor reps to email me directly. This may 
lead to an opportunity for companies to participate in this project.


here is what I have thus far:

Thermo Fisher
General Data Healthcare
Sakura
Leica
TBS (triangle biomedical)

I know that Ventana markets a comprehensive software product that is used for 
specimen management but I don't know what that includes.
I thought Dako may have had a competing product but I am unsure about this.

I also know that in the past there were different versions of cassette printers 
from RA Lamb that were offered through different vendors, so for example the 
TBS and old Shandon/Thermo MicroWriters were different versions of the Lamb 
cassette printers.

any information that you can offer will be greatly appreciated.
thanks

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974




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[Histonet] Has anyone else noticed

2011-12-19 Thread Della Speranza, Vinnie
 
Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.  

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

 



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FW: [Histonet] Has anyone else noticed

2011-12-19 Thread Della Speranza, Vinnie



From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu
Sent: 12/19/2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





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RE: [Histonet] Has anyone else noticed

2011-12-19 Thread Della Speranza, Vinnie
Actually Tony, by the time you get to the bottom of the bottle, you probably no 
longer care what color it is ! 


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

 


-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] 
Sent: Monday, December 19, 2011 5:04 PM
To: Della Speranza, Vinnie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Has anyone else noticed

I keep trying to verify this scientific discovery but everything is blurry by 
the time I get to the bottom of the bottle, Oh sorry I need to look in the 
glass?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie
Sent: Tuesday, 20 December 2011 3:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Has anyone else noticed




From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu
Sent: 12/19/2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





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Views expressed in this message and any attachments are those of the individual 
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[Histonet] Real Pathologist of Genius

2011-06-06 Thread Della Speranza, Vinnie
I've not seen this posted here. I apologize if this is a repeat.

If you've not seen this, it is a MUST SEE, especially if you do IHC
You'll need speakers

http://www.youtube.com/watch?v=-fsn0GrzX5Ufeature=player_embedded


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

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[Histonet] pricing for EM on kidney

2011-06-03 Thread Della Speranza, Vinnie
I've been asked to get pricing for Electron Microscopy on kidneys from labs 
that accept out reach samples. we are interested in your fee for EM only and 
not a complete kidney work up.

In addition to pricing for kidney samples provided in gluteraldehyde, I'll also 
need to know if your pricing is different if you are asked to work from a 
paraffin block.

thank you,

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, SC 29425
ph (843) 792-6353
fax (843) 792-8974





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[Histonet] RE: off topic for the guys

2011-05-27 Thread Della Speranza, Vinnie
OK, I saw Andrea's post and after all , it is Friday.
I saw a recent episode of CSI-New York that had a cochineal beetle as part of 
the crime scene evidence about a sniper. Anyway, it got me wondering, anyone 
know if automatic transmission fluid is colored with cochineal (carmine) ?? (I 
had to make this guy related and don't know any girls working on their 
transmissions)

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
  
   
  Learn more about Histotechnology Professionals Day at 
www.nsh.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Thursday, May 26, 2011 3:29 PM
To: HISTONET
Subject: [Histonet] off topic/on topic

This is mostly for the girls - I dropped into the Ulta store yesterday to pick 
up a few things and the sales person wanted me to try this somewhat new product 
by Benefit. It was a lip and cheek tint - came in a little bottle - looked like 
mucicarmine working solution. I looked at the box that it came in and sure 
enough, one of the first ingredients was CARMINE! So I had to relate a few of 
the stories about beetles and Carmine and pirates that I learned from reading 
A Perfect Red recommended by Dick Dapson...wonder if I bored her? Maybe she 
won't bother me the next time I'm rushing through the store trying to get my 
stuff and get out of there.
Andi

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RE: [Histonet] Mohs Key Performane Indicators (Recuts)

2011-04-26 Thread Della Speranza, Vinnie
I would think that the reason for the recut is relevant to the discussion. We 
have a multi surgeon Mohs Surgery clinic and I've tracked recut requests by 
surgeon and the data illustrates what I already knew. We have one surgeon who 
prefers to go deeper into virtually every block. In this instance it is not a 
quality indicator but rather an indicator of practice style.

It sounds like your environment is a single surgeon practice which should 
simplify developing your own quality standards. I am not aware that such 
standards exist but you may consider contacting the Mohs College with your 
question.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
  
   
  Learn more about Histotechnology Professionals Day at 
www.nsh.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martino T.
Sent: Thursday, April 21, 2011 5:22 PM
To: Histo Net
Subject: [Histonet] Mohs Key Performane Indicators (Recuts)


Hello,
 
I work for a Mohs surgeon who wants to establish key performance indicators 
(KPIs) for the mohs techs to evaluate daily performance. Does anyone have any 
information of surveys or standards for the number of recuts per day or per 
block as a measure for quality Mohs sectioning? She's working with a standard 
that 4 or less recuts in a day (average 30 to 50 blocks per day) is quality 
work. Has anyone come up with standards in this regard for Mohs sectioning? 
 
Thanks in advance,

Martino Tanner

Life can either be accepted or changed. If it is not accepted, it must be 
changed. If it cannot be changed, then it must be accepted.

  
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[Histonet] S100 primary antibody suitable for frozen sections?

2011-04-26 Thread Della Speranza, Vinnie
I would appreciate hearing from anyone who has discovered an S100 primary 
antibody that is confirmed to work on frozen sections using a conventional IHC 
stain protocol. This will be utilized in a busy Mohs Surgery laboratory where 
staining time must be kept as brief as possible. 

The commercially available antibodies I've seen for this antigen are intended 
for FFPE tissues and initial attempts to adapt them to frozen sections have 
been unsuccessful. 

our surgeons prefer to avoid post fixation prior to staining although I'm 
willing to re-visit this if it is my only option.

 Thank you everyone.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
  
   


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[Histonet] RE: Feulgen Stain for DNA

2011-01-10 Thread Della Speranza, Vinnie
Feulgen demonstrates the deoxyribose which is in the backbone of each helical 
strand. It shouldn't matter if single or double stranded.
Hydrolysis is the key step and can be overdone. Textbooks list using hot 1N HCl
Many years ago Jules Elias and I co-authored a paper where we demonstrated that 
the use of 5N HCl at room temperature provided more consistent results 

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
  
   
  Learn more about Histotechnology Professionals Day at 
www.nsh.org
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Friday, January 07, 2011 4:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Feulgen Stain for DNA

Hello Everyone

 

I have tried to find this answer on the web and in references but I need
some help.  With respects to the Feulgen stain, how sensitive is it? Can
it detect smaller fragments of DNA? Does it have to be double stranded
or will single stranded stain as well?  Thanks in advance.

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

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[Histonet] c-kit mutation for Melanoma

2011-01-04 Thread Della Speranza, Vinnie
I'm interested in learning what labs you are using for c-kit mutation for 
Melanoma. I'm told this testing is different for c-kit in other organ systems 
and that labs performing c-kit may not be equipped to work up the melanoma 
variant



Thank you for any light you can shed on this.



Vinnie Della Speranza

Manager for Anatomic Pathology Services

165 Ashley Avenue Suite 309

Charleston, SC 29425

tel. 843-792-6353

fax. 843-792-8974






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RE: [Histonet] friable or crumbly O.C.T.

2010-08-09 Thread Della Speranza, Vinnie
I'm guessing that liquid nitrogen or dry ice temperature is too cold for 
sectioning OCT.
OCT cuts well down to about -25 degrees C. 
Liquid Nitrogen is about -160 degrees C. I believe dry ice is in the same temp 
range at LN2

You will want to give the OCT blocks the opportunity to warm up to cryostat 
temperature before attempting to section them. Leave your frozen blocks in the 
cryostat for 30-60 minutes before sectioning to allow them to come to optimum 
temperature

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce W 
Brodersen
Sent: Friday, August 06, 2010 2:26 PM
To: histo...@pathology.swmed.edu
Subject: [Histonet] friable or crumbly O.C.T.



Anyone have an explanation as to why OCT would be friable or crumbly for
sectioning?  Here's how it was used.
Thanks.

We held the plastic 'tray' with the tissue in the compound just over the
liquid nitro for 30sec-1min, until it was opaque and white (frozen) and
then dipped the tray into the liquid nitro for 20-30sec., placed in small
bags and then into a cooler with dry ice until shipping.


Bruce W. Brodersen, DVM, PhD
University of Nebraska Veterinary Diagnostic Center
1900 N. 42nd Street
Lincoln, NE  68583-0907

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[Histonet] New online Histology study program !!

2010-07-14 Thread Della Speranza, Vinnie
Greetings everyone,



Occasionally I see posts from individuals seeking advice on where they can get 
the instruction they need to become a certified histotech. It is for this 
reason that I am sharing this information.



The Histotechnology School at MUSC is beginning an online instructional program 
for students who would not find it feasible to come to Charleston to study with 
us directly. Our school began in 2004 and is NAACLS accredited. The online 
study option is a new option of our program.



Our school's website can be accessed at 
www.musc.edu/histoprogramhttp://www.musc.edu/histoprogram



I should point out that our program is for individuals seeking to become 
histotechnologist  (HTL) certified. Applicants must have their baccalaureate 
degree in order to be eligible for enrollment.



Below I am providing information I received from our school's program director, 
Karen Geils, and ask that you share it with anyone who might benefit. I've also 
included Karen's contact information so that she can be reached for further 
information. I hope that you will contact Karen if our program can be of any 
help to you.


All didactic material will be delivered online via asynchronous media, and 
clinical experience will be provided by the affiliate laboratory.  The course 
management system, WebCT will be used to deliver assignments, lecture material, 
and tests. Students will be required to have access to a personal computer with 
internet.

Minimum hardware standards (Mac or PC):


PC Software

Mac Software

CPU with minimum of 2.0GHz
1 GB RAM (2 GB if using Windows Vista)
80GB Hard Drive
2 USB 2.0 ports
IEE 1394 Firewire
CD/DVD RW +/- Drive
Sound Card (external or internal speakers)
Microphone headset


Windows XP, Vista, or 7
Microsoft Office
Adobe Reader
Internet Browser - IE or Firefox

Mac OS X version 10.4 or 10.5
Microsoft Office for Mac
Adobe Reader
Internet Browser - Firefox


The fee is $3600.00 and includes the cost of textbooks. Clinical sites are 
responsible for laboratory supplies and equipment. MUSC will verify that the 
clinical experience is comparable to that of the MUSC onsite program.

Karen Brinker Geils, MS, HT(ASCP), CT(ASCP)
Director, Histotechnology Program
Department of Pathology and Laboratory Medicine
Medical University of South Carolina
843-792-4013
brink...@musc.edumailto:brink...@musc.edu






Vinnie Della Speranza

Manager for Anatomic Pathology Services

Medical University of South Carolina

165 Ashley Avenue  Suite 309

Charleston, South Carolina 29425

Tel: (843) 792-6353

Fax: (843) 792-8974


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RE: [Histonet] Formalin and Xylene odor containment

2010-07-09 Thread Della Speranza, Vinnie
I don't have any expertise in this area however I recall reading about the 
controversies of ozone generators. You would do well to do a bit of internet 
research before you take this leap. If your facility has a safety officer I'd 
definitely determine if there is an institution position on ozone generators.

Here is one link for information but you shouldn't have difficulty coming up 
with others.

http://www.epa.gov/iaq/pubs/ozonegen.html


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joao Pessoa
Sent: Friday, July 09, 2010 3:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin and Xylene odor containment

All,

We have recently been using a new piece of demo equipment to control
formalin, xylene and other odors.  The unit is an air purifier from BioZone
Scientific.  Our ventilation is good in all areas of our lab and we have
always been found to be well below the particulate count allotment for
xylene, formalin, and other compounds.  However, many area of the lab still
had a heavy chemical smell (like a beauty salon, they say).  This smell goes
away with the BioZone unit, which uses UV light and ozone to break down the
organic compounds.  Does anyone else has experience with BioZone?  We are
thinking about buying it, but I wanted to hear the opinion of others.

Cheers,

Joao

Histo Tech
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[Histonet] RE: possible AFB contaminant

2010-05-21 Thread Della Speranza, Vinnie

Do you use DI water in your flotation baths ?

If your DI system is not already so equipped, install a 0.5 micron filter where 
you withdraw the water and be sure to change this filter monthly.

I'm willing to bet the bugs are on the slides before the stain is performed 


Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
debra.or...@uchospitals.edu
Sent: Thursday, May 20, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] possible AFB contaminant

Please help, we have had an increase in demands for repeats on our AFB stain. 
Our pathologists have been noticing what they believe are false positives on 
our slides. We perform our AFBs on the Ventana Nexus and some are done by hand. 
Because of this problem, we have been doing side by side comparisons using the 
Nexus and hand stain.
We still have complaints. We have made new reagents, changed kits, tested our 
DI water sytem and have different techs cutting using different waterbaths.
Any advice or comments would be greatly appreciated.
 
Thanks Debi


This e-mail is intended only for the use of the individual or entity to which
it is addressed and may contain information that is privileged and confidential.
If the reader of this e-mail message is not the intended recipient, you are 
hereby notified that any dissemination, distribution or copying of this
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notify the sender and destroy all copies of the transmittal. 

Thank you
University of Chicago Medical Center 


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RE: [Histonet] RE: DAKO Autostainer Link 48/FLEX system

2010-04-19 Thread Della Speranza, Vinnie
If you prefer not to post to the list I'd appreciate hearing about your pros 
and cons off list as we will be evaluating this instrument. Thank you Loralee.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy Bulmer
Sent: Friday, April 16, 2010 9:40 AM
To: JeanTaylor; 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu'; 
Loralee AMcMahon
Subject: Re: [Histonet] RE: DAKO Autostainer Link 48/FLEX system

Hey Loralee,
 
Could you post a few of the pros and cons?  Do you like the new system?

Cynthia Bulmer HT(ASCP)QIHC
IHC Supervisor, CTPL
Waco, TX

--- On Thu, 4/15/10, McMahon, Loralee A loralee_mcma...@urmc.rochester.edu 
wrote:


From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu
Subject: [Histonet] RE: DAKO Autostainer Link 48/FLEX system
To: Taylor, Jean jtay...@meriter.com, 'ih...@googlegroups.com' 
ih...@googlegroups.com, 'histonet@lists.utsouthwestern.edu' 
histonet@lists.utsouthwestern.edu
Date: Thursday, April 15, 2010, 8:12 PM


I have upgraded to the new autostainer links.  I have four of them. There are 
many pros and many cons.  If you have any specific questions I'd be happy to 
answer. 


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean 
[jtay...@meriter.com]
Sent: Thursday, April 15, 2010 3:56 PM
To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] DAKO Autostainer Link 48/FLEX system

Hi Everyone,

I would like to hear (pros and cons) from labs that have this instrument or 
have looked at it. We currently use 2 of the oldest Dako autostainers and are 
in need of updating. Any feedback would be appreciated.

Thanks,
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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[Histonet] RE: Coverslippers

2010-03-26 Thread Della Speranza, Vinnie
For those who are not aware, the Dako unit is apparently the same coverslipper 
previously marketed by Surgipath. This information may lead to greater 
responses since Dako has only recently acquired this unit as I understand it.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debora Probst
Sent: Friday, March 26, 2010 10:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Coverslippers

Yes, I too would like to know if any one has ever used The Dako
coverslipper and if they liked it or not.

 

Debora Probst HT

Columbus Regional

Columbus, Ga.

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[Histonet] television coverage of Histotechnology Professionals Day

2010-03-11 Thread Della Speranza, Vinnie
I thought some of you might be interested to learn that our histo lab staff 
were interviewed yesterday by the Charleston CBS affiliate station Live 5 News. 
The interview was telecast last evening at about 5:30 pm so we succeeded in 
getting histology on the air waves.

The news reporter showed up with almost no warning while I was enroute to the 
state capital with Wanda Smith to meet with our governor. I'm thrilled that our 
techs were in the spotlight. They and all of you, deserve to be recognized for 
all that you do to enhance our quality of life.

If you'd like to see the video clip, go to 

http://www.live5news.com/Global/story.asp?S=12118805

on that page, off to the right, you will see the phrase
Lab techs recognized for work behind the scenes

Click on that phrase and the video will open. Turn up your speakers as the 
volume is a bit inconsistent.

The telecast was perfect in every way. The video clip does not include two 
teasers that preceded the actual segment, you know the ones where they say 
when we return, histotechs at MUSC...



Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

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RE: [Histonet] RE: Eliminating the edge effect in IHC/IF

2010-01-12 Thread Della Speranza, Vinnie
I think we'll agree that there are different scenarios so that the solution is 
not a one size fits all. For example, darkened staining around the periphery of 
needle core biopsies is not uncommon with even tinctorial stains, often thought 
to be the result of drying of the tissue during the collection of the sample. 
Years ago I came across an article maintaining that the dark staining on the 
periphery of needle cores was in fact due to the trauma of the needle cutting 
into the sampled organ. I've since forgotten the author's name and wish I could 
get my hands on that reference.

So I agree with Chris that there doesn't appear to be one simple answer to 
prevent this artifact and while fixation may contribute in some circumstances 
it's unlikely to be the remedy for all.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of C.M. van der 
Loos
Sent: Tuesday, January 12, 2010 2:55 PM
To: histonet@lists.utsouthwestern.edu
Cc: k...@prosci-inc.com
Subject: [Histonet] RE: Eliminating the edge effect in IHC/IF

Hi all,We also have observed this phenomenon many times. But sorry Colleen and 
Rene, I don't believe that an fixation issue is the explanation why the edges 
are sometimes stronger than the rest. To my opinion this is a bit too easy. One 
of my explanations is that the immuno reagents tend to stick to the edges of 
the tissue section, especially when no Tween20 is involved. As a result the 
outer edges might become a little dry during incubation times and cause darker 
mostly non-specific staining. Always try to cover a section completely, 
including a rim around the section. However, to be honest, I am sure my 
explanation is certainly not always appropriate. Anyone 
elseCheers,ChrisChris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
phone:  +31 20 5665631
 From: Rene J Buesa rjbu...@yahoo.com
Subject: Re: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu, Karen Cai k...@prosci-inc.com

Usually that is the result of incomplete fixation. Check your fixation protocol.
Ren� J.

--- On Mon, 1/11/10, Karen Cai k...@prosci-inc.com wrote:


From: Karen Cai k...@prosci-inc.com
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet@lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM


Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?

Your kind help is greatly appreciated,


Thanks in advance,

Best,
Karen

Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
http://www.prosci-inc.com www.prosci-inc.com
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RE: [Histonet] FNA code

2009-12-31 Thread Della Speranza, Vinnie
Mary, I'd have to disagree.



88173 has both a technical and professional component. This is well documented 
in a resource your dept might find very beneficial entitled Pathology Service 
Coding Handbook which is authored by Dennis Padgett, a noted coding authority.



It is customary to have a cytotechnologist screen FNA smears or Thin Prep 
slides prior to the slides going to sign out with the pathologist. And of 
course the hospital incurs supply expenses as well as labor expense associated 
with the preparation of the specimen(s) that will be interpreted



The rule of thumb is as follows:



88173 should be dropped for every Fine Needle Aspirate. This represents all of 
the work in the laboratory (including prep and staining) that leads to the 
interpretation both technical and professional



88172 is dropped only if the procedure is attended by a pathologist or 
cytotechnologist to render an adequacy during the procedure. This presumes that 
the aspirate is not being performed by the pathologist but another clinician, 
radiologist, etc.



It is appropriate to drop additional codes to 88173, such as codes for cell 
block (88305), special stains (88312;88313), IHC (88342) or thin layer 
preparation of the aspirate (88112 ) however it may interest you to know that, 
according to Padgett,  Medicare will never pay 88112 when it is added onto 
88173. so for example, if you do direct smears and thin prep on an FNA 
specimen, Medicare will still only pay for 88173.



Other payors may vary and for simplicity it may be simpler for labs to drop 
88112 whenever thin prep is done on a FNA specimen without regard to payor 
however you ought to consult with your institution's legal or compliance 
officer.



I highly recommend the reference I mentioned above. You wouldn't believe the 
complexity of the interpretations for these codes and Padgett points out 
instances where CAP's interpretation disagrees with AMA's.  Padgett devotes 21 
pages of discussion on FNA coding



Vinnie Della Speranza

Manager for Anatomic Pathology Services

165 Ashley Avenue Suite 309

Charleston, SC 29425

tel. 843-792-6353

fax. 843-792-8974





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary McCoy
Sent: Thursday, December 31, 2009 10:23 AM
To: dkb...@chs.net; histonet-boun...@lists.utsouthwestern.edu; Carolyn Demarinis
Cc: histo...@pathology.swmed.edu
Subject: Re: [Histonet] FNA code



There is no Technical component to 88173, so how do you charge for the 
technical preparation of the ThinPrep?



Mary McCoy HTL(ASCP)

Supervisor of Pathology Services

Lakeland Regional Health System

St. Joseph MI 49098

(269) 982-4891

mmc...@lakelandregional.org





 dkb...@chs.net 12/30/2009 9:42 AM 

88173



Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical

Center I

200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F:

804-765-5582 l dkb...@chs.net















Demarinis, Carolyn cdemari...@saratogacare.org

Sent by: histonet-boun...@lists.utsouthwestern.edu

12/30/2009 08:18 AM



To

histo...@pathology.swmed.edu

cc



Subject

[Histonet] FNA code













Which CPT code are labs using for fine needle aspirations that are

processed using thinprep technique -

FNA interpretation and report-88173 or

thinprep non-gyn 88112?  Thank you.







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any review, disclosure, dissemination, distribution or copying

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RE: [Histonet] Ventana Stainer

2009-12-10 Thread Della Speranza, Vinnie
Jimmy has informed me that he resides in the Cleveland area. Can anyone in that 
area that has a Ventana stainer permit him a visit ??

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jimmy A
Sent: Tuesday, December 08, 2009 1:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana Stainer

 
 
   
  Hi
    I am looking for a lab that can allow me to spend one or two days to 
properly see how the ventana autostainer works.
 I am an histotech working here in the US. I am interested in learning  the 
operation of 
the ventana immunostainers. I will appreciate it, if one of the histology/ihc 
labs 
could grant me this great favour.
  Hoping to hear from you guys asap.
 
 Jimmy.



  
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[Histonet] B Plus fixative on bone marrow cores

2009-09-24 Thread Della Speranza, Vinnie
I would appreciate comments from anyone using this fixative for bone marrow 
cores. Specifically I am interested in learning how long you are fixing your 
samples in B Plus.
Manufacturer recommends at least two hours but we are seeing artifacts that 
concern me that 2 hrs may be insufficient.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 



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RE: [Histonet] Pathologist Consultation Send out

2009-09-08 Thread Della Speranza, Vinnie
Dr. Cartun's remarks describe the process followed at our facility.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Sunday, September 06, 2009 10:59 AM
To: histonet@lists.utsouthwestern.edu; rick.garnh...@memorialhealthsystem.com
Subject: Re: [Histonet] Pathologist Consultation Send out

Most pathologists that bill separately have a contract with the hospital to 
provide services.  Therefore, in my opinion, if the pathologist can't provide a 
diagnosis and the case is sent out, the pathologist is responsible for the 
bill.  However, if the case is sent out at the request of the patient or 
clinician (after the pathologist has established a diagnosis) then the patient 
(or her/his insurance) or the clinician is responsible for the bill.  The 
hospital should not have to pay for these requests.  

Richard

Richard W. Cartun, Ph.D.
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

 rick.garnh...@memorialhealthsystem.com 9/4/2009 1:07 PM 

If your Pathologists bill for the professional component privately, and your 
hospital bills for the technical component, who pay for expert
consultations of slides sent out?


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnh...@memorialhealthsystem.com 



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FW: [Histonet] clearing agent for gross specimens

2009-08-21 Thread Della Speranza, Vinnie


Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-Original Message-
From: Shawn Leslie [mailto:lesl...@vetmed.ufl.edu] 
Sent: Friday, August 21, 2009 12:21 PM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] clearing agent for gross specimens

Hi Vinnie,
We used to just use Glycerin...It would clear the fat quite
nicely.then the lymph nodes could be visualized

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della
Speranza, Vinnie
Sent: Friday, August 21, 2009 12:16 PM
To: Histonet
Subject: [Histonet] clearing agent for gross specimens

I would appreciate hearing from anyone who routinely utilizes a clearing
agent in the gross room to clear fat from tissues to allow lymph nodes
to be visualized.

I am aware of Dissect Aid but would like to learn of other comparable
products. 
If individuals are using home made solutions are will to share their
formula I would appreciate receiving that information as well.

Our primary interest is speed of clearing for turnaround time reasons.

I am in the midst of a Histosearch search but it is slow going because
most discussions refer to tissue processing or staining so it's like
looking for a needle in a haystack


Thank you all

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 


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FW: [Histonet] clearing agent for gross specimens

2009-08-21 Thread Della Speranza, Vinnie


Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-Original Message-
From: Jason McGough [mailto:jmcgo...@clinlab.com] 
Sent: Friday, August 21, 2009 12:25 PM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] clearing agent for gross specimens

We use a home made solution that works very well for lymph node
visualization. Here is our formula:

5L ETOH
3.4L distilled water
1.6L 37-40% Formaldehyde
1L Glacial Acetic Acid

Let me know if I can be of further assistance.

Jason McGough HT(ASCP)
Account Representative - Anatomic Pathology
Clinical Laboratory of the Black Hills
2805 5th Street Suite 210
Rapid City, SD 57701
605-343-2267 Ext 127
605-718-3779 (Fax)
jmcgo...@clinlab.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Della
Speranza, Vinnie
Sent: Friday, August 21, 2009 10:16 AM
To: Histonet
Subject: [Histonet] clearing agent for gross specimens


I would appreciate hearing from anyone who routinely utilizes a clearing
agent in the gross room to clear fat from tissues to allow lymph nodes to be
visualized.

I am aware of Dissect Aid but would like to learn of other comparable
products.
If individuals are using home made solutions are will to share their formula
I would appreciate receiving that information as well.

Our primary interest is speed of clearing for turnaround time reasons.

I am in the midst of a Histosearch search but it is slow going because most
discussions refer to tissue processing or staining so it's like looking for
a needle in a haystack


Thank you all

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974



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[Histonet] FW: clearing agent for gross specimens

2009-08-21 Thread Della Speranza, Vinnie


Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-Original Message-
From: Cazares, Ruth [mailto:rcaza...@schosp.org] 
Sent: Friday, August 21, 2009 12:58 PM
To: Della Speranza, Vinnie
Subject: RE: clearing agent for gross specimens

Vinnie,

We make our own Davidson's fixative, and we also tried Dissect Aid. Comparing 
the two, our PA and our pathologists liked the homemade Davidsons fixative 
better than the Dissect Aid.


Ruth Cazares, HT (ASCP)
Histology Supervisor
Department of Pathology
Swedish Covenant Hospital
5145 North California Ave
Chicago, IL 60625

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie
Sent: Friday, August 21, 2009 11:16 AM
To: Histonet
Subject: [Histonet] clearing agent for gross specimens

I would appreciate hearing from anyone who routinely utilizes a clearing agent 
in the gross room to clear fat from tissues to allow lymph nodes to be 
visualized.

I am aware of Dissect Aid but would like to learn of other comparable products.
If individuals are using home made solutions are will to share their formula I 
would appreciate receiving that information as well.

Our primary interest is speed of clearing for turnaround time reasons.

I am in the midst of a Histosearch search but it is slow going because most 
discussions refer to tissue processing or staining so it's like looking for a 
needle in a haystack


Thank you all

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974



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RE: [Histonet] Freezing in Hexane

2009-08-13 Thread Della Speranza, Vinnie
Hi Dorothy,
I've not used hexane. I think 2-methly butane is more commonly used if you are, 
as I suspect, snap freezing in a solvent chilled in liquid nitrogen. The 
solvent just needs to have a low freezing point. 2-methyl butane freezes at 
about - 150 degrees C. I do not know if hexane has a lower freezing point but 
you can determine that with an internet search.

My point in all of this is that you may have other choices for snap freezing if 
you can't get an answer to your original question re hexane as long as you are 
able to freeze the needed temperature.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tracz...@aol.com
Sent: Wednesday, August 12, 2009 12:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Freezing in Hexane

I have a procedure that refers to freezing a tissue sample in chilled  
Hexane.  When I look in a chemical catalogue I find there are several  formulas 
listed.  Any direction on which one to use would be greatly  appreciated.  
Thank you.
Dorothy
 
Dorothy Murphy Traczyk
Murphy-Traczyk  Associates LLC
PO Box 602
Point Pleasant, NJ 08742
_doro...@mtahistology.com_ (mailto:doro...@mtahistology.com) 
www.mtahistology.com
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RE: [Histonet] Re: Eosin in alcohol

2009-07-01 Thread Della Speranza, Vinnie
Jennifer, is it possible that the lab asking you to use cobalt blue is using a 
fluorescence technique on the prostates? We've used eosin for years and not 
seen any impact on IHC staining for light microscopy.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson
Sent: Wednesday, July 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin in alcohol


We have used Eosin in the last 95% alcohol on the tissue processor for several 
years.  I usually add approximately 5 ml to the full jug.  It is a great tool 
to use for embedding.  However, we received a letter from the lab that we send 
our prostate biopsies to saying that it was undesirable because it interfered 
with their immuno staining.  They sent us some cobalt blue to use in the place 
of eosin along with mixing instructions and the whole batch of tissues came out 
such a dark blue.  There is no delineations in the color of the blue and I 
found it to be useless for helping to embed.  I would rather do without 
anything than use cobalt blue.  I guess the point of my rambling is, Eosin is a 
wonderful tool to use unless you are doing immunos on prostate biopsies.  

Thanks,

Jennifer Johnson, HTL (ASCP) 

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RE: [Histonet] tracking turnaround time of intraoperative consultations

2009-06-24 Thread Della Speranza, Vinnie
Tom, are the times you've listed benchmarks your facility has established for 
it's operation? I'm not aware of any national benchmarks using those numbers 
but want to be sure I haven't missed something.

Also, where is your frozen lab located in relation to the OR? Are you located 
within the OR area or elsewhere? And does your 15 minute benchmark include 
pre-analytical specimen transport time?

Sorry for all of the questions, just want to be sure we are all comparing 
similar circumstances.
thanks

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] 
Sent: Tuesday, June 23, 2009 6:23 PM
To: Garrison, Becky; Della Speranza, Vinnie; histonet
Subject: RE: [Histonet] tracking turnaround time of intraoperative consultations

This is basically how we have always done. On scheduled frozens we have 15 
minutes for turnaround, unscheduled 30 minutes.


Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky 
[becky.garri...@jax.ufl.edu]
Sent: Tuesday, June 23, 2009 5:51 PM
To: Della Speranza, Vinnie; histonet
Subject: RE: [Histonet] tracking turnaround time of intraoperative  
consultations

We have just started tracking from order to sign out for frozen
sections.

(In addition, frozens are tracked from receipt in pathology to sign out
using the CAP guidelines).

The trouble with the electronic order (in our institution) is that the
OR may place the pathology order in hospital computer system early in
the surgery so that the order time that prints on the requisition is
substantially different than the actual collect time.

We have resolved this by having the OR staff write the actual collect
time
on the requisition and initial it.  This collect time is also noted in
the OR documentation notes for the surgery.  When OR forgets to note
collect time manually on the requisition (and they do), I call back and
have them  look up and verify the collect time.

This was started with the cooperation and support of the OR
administration.

For the pathology accession staff, this means they can not use the order
time that crosses the interface to the LIS (lab computer system)  but
must enter the handwritten time as noted on the requisition.

We have set a goal of 40 minutes from frozen order to sign out. This may
be lowered to 30 - 35 minutes depending on how our data looks over
several months.  Our pathology dept. is located on the first floor and
the OR on second floor of same building.

As for noting collect times for multiple specimens, same case: We have
always required the OR to generate a requisition for each container.
The collect time is written on each requisition.  This is no different
than
writing the collect date/time and initials that nursing/phlebotomy does
for each tube of blood drawn hospitalwide.

Would be interested in hearing from others on how this is handled.

Becky Garrison
Pathology supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-24-6237



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della
Speranza, Vinnie
Sent: Tuesday, June 23, 2009 4:02 PM
To: histonet
Subject: [Histonet] tracking turnaround time of intraoperative
consultations

CAP utilizes the term intraoperative consultation to describe the
utilization of frozen (cryo) sections to provide a rapid diagnosis back
to a surgeon in the operating room.

The CAP checklist requires a turnaround time of 20 minutes for single
specimen submitted for intraoperative consultation. My understanding is
that the turnaround time is measured from the time the sample is
received in the laboratory until the time the report is issued to the
surgeon.

Is anyone tracking or measuring turnaround time from the time the
consult is ordered in/by the Operating Room until the time the result
is issued?
If so, would you share how you are able to determine the time the test
was ordered  and to what extent you have elicited the cooperation of
Operating Room personnel.

We receive many complex surgical cases and our intraoperative consults
frequently consist of multiple surgical samples from the same patient
arriving in the lab at the same time. Our head and neck cases, for
example, consist of 6-8 biopsies that are sent to pathology at the same
time. In this example, we have no knowledge of which biopsies was
excised first or last and because the surgeon chooses to allow multiple
samples to accumulate before sending them all off to the lab, it's clear
that the true pre-analytical time will not be the same for each
sample.

If you

RE: [Histonet] Re: tracking turnaround time of intraoperative consultations

2009-06-24 Thread Della Speranza, Vinnie
Thanks Dr. Richmond. CAP's turnaround time requirement for frozen sections is 
unchanged.

My question was prompted by the fact that we have an individual internal to our 
organization pushing for measuring turnaround from time of order to time result 
is issued, which muddies the water, at least for us as we do not have 
electronic ordering from the OR. This is prompted by JCAHO's requirement that 
turnaround time for critical tests be measured (Frozen section is considered a 
critical test by this organization)

As far as I know, there is no national standard to be met if one measures 
turnaround from time of order, so the data then is up to the institution's 
interpretation for what is acceptable. 

One of the respondents indicated that they consider the time the sample gets to 
pathology as the time the test was ordered. Of those who responded to my query, 
one lab has electronic order entry and is just beginning to track both the in 
lab turnaround time and the time from order to result. 

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Wednesday, June 24, 2009 1:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: tracking turnaround time of intraoperative consultations

Vinnie Della Speranza, Manager for Anatomic Pathology Services Medical
University of South Carolina
Charleston SC asks about tracking turnaround time of frozen sections (note
that not every intraoperative consultation requires a frozen section).

The few services I've worked on that attempted to track turnaround time
timed them from time of receipt in the laboratory (using a time stamp for
that) to telephoning the report (the pathologist had to write down the time
on the hand-scribbled report). The prescribed maximum turnaround was 20
minutes, which is pretty easy to meet. Cases with multiple frozen sections
were not timed.

Has there been some change in the CAP requirements for recording turnaround
time of frozen sections in the last three years?

Bob Richmond
Samurai Pathologist
Knoxville TN
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RE: [Histonet] Dako, Thermo and others

2009-03-12 Thread Della Speranza, Vinnie
I wasn't aware that Thermo now has the rights for the PSLIM. Thanks for posting 
this Victor. I did have an interest in this printer at one time, but based on 
this information I won't be considering it.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Thursday, March 12, 2009 11:02 AM
To: jeff
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Dako, Thermo and others

Jeff,

It is not just Dako, but many vendors have jacked up the cost of goods. 
Let's take the PSLIM from Accuplace. About a month ago you could 
purchase this for $5K. Now that Thermo has acquired distribution rights 
it costs over $10K and nothing has changed. To me, it is better to sell 
10 at $5K than 5 at $10K. We were ready to purchase several more of 
these, but have to reconsider our options now. No wonder Healthcare 
costs are out of control.

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
vic...@pathology.washington.edu
206-598-2792
206-598-7659 Fax
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then destroy all copies of the message and any attachments.



jeff wrote:
 I was wondering if anyone else is being blackmailed by Dako to sign a 
 contract with them. We have enjoyed our relationship a number of years buying 
 there product as we need it. We also have changed detection kits as we where 
 advised. Now they are telling us that if we do not go  with price per slide 
 they will raise our costs of purchasing our supplies. Example  EnVision + 
 dual link system last year cost 1,580.70 this year 3,300 and that this will 
 go in effect March 1st ( just talked to the rep about this Tues) Just 1 
 example. S. Has anyone else had this happen to them and what did you do?  
  Thanks  Jeff

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RE: [Histonet] CPT Coding question 88342 PIN 4 cocktail

2009-03-11 Thread Della Speranza, Vinnie
Disclaimer: I do not consider myself to be a coding expert. Take my opinions 
with a grain of salt.

I've never seen coding for softening of keratin therefore I don't see how you 
could charge for it. This is not decalcification and should not be represented 
as such. Until such time as a code appears (don't hold your breath) the KOH or 
whatever technique you employ is for your convenience to make a block more 
cuttable (is that a word?).

There is no rule that technical and professional charges must be the same. They 
are reimbursed differently, sometimes the technical having a higher 
reimbursement than the professional. You are free to set your charges for each 
where you see fit.

I believe that your charges for prostate IHC have to be justified by the 
pathology. In your example, some of those cores may be clearly benign and there 
would be no basis for charging IHC for eight cores if only one or two contain 
the lesion. In your case, by having four cores in one block, you benefit by 
keeping your expenses down however I do believe that your IHC charges should 
(must?) be based on the location of the lesion under study. It would seem to me 
that charging for IHC on eight cores when the report describes a lesion in only 
one is asking for trouble 

You are correct that you can charge 88342 x 3 for the PIN 4 markers as each is 
separate and can be visualized distinctly from one another.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's
Sent: Tuesday, March 10, 2009 9:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CPT Coding question 88342 PIN 4 cocktail

We started staining prostate needle bxs w/ PIN 4 (triple stain). 
1. My understanding is that we can bill for 88342 x 3 (per specimen) if a 
comment is made on the results of the nuclear staining and cytoplasmic staining 
of the DAB and the staining of the Vulcan red. The key is documentation in the 
report. This is simple to understand when you rec'd 1 specimen/container, but 
we rec'd:

A. Right prostate Bx - Red stained bx: apex
 Green stained bx: base
 Blue stained bx: midgland
 Yellow stained bx: transition
submitted in one Cassette A.

B. Left  prostate Bx - Red stained bx: apex
 Green stained bx: base
 Blue stained bx: midgland
 Yellow stained bx: transition
submitted in one Cassette B.

2. If the pathologist needed  this stain on every specimen, it would be 8 
separate identifiable specimens x 3 (separate identifiable stains in the 
cocktail) , therefore88342  x 24, even though it is only 2 blocks.

3. If the pathologist was only interested in this stain on on the Apex in A and 
B it would be 2 specimens x 3, therefore 88342 x 6 for the same 2 blocks (even 
though it stains all 8 specimens, only 2 of the 8 are in need of this stain).

   Another words, we can't charge the technical component until we find out how 
many specimens the pathologist is looking at in a slide (professional 
component), even though it is the same amount of work and reagents.  Does this 
sound right?

4. Is the technical charge always the same as the professional charge?


Another unrelated question - We can bill for decalcification, but is there a 
billing code for  KOH in the same manner to soften and treat toenails before 
processing? It is documented in the Path report.
I know this has been discussed in the archives, but there seems to be 
conflicting opinions. Do we know for sure?
Thanks


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RE: [Histonet] Practical Exam

2009-02-23 Thread Della Speranza, Vinnie
I've returned from a day off to an inbox stuffed with messages about the 
decision to eliminate the practical for the certification exam. I apologize if 
someone has already commented on this as I've got lots of reading to do in 
order to catch up.

A very large reason the practical exam was eliminated was the very high pass 
rate. I don't have hard data to give you but it is my understanding that the 
practical pass rate exceeded 95% (my current guestimate based on my 
recollection of information I was given a few years ago)  which to a testing 
agency implies that the exam is unnecessary if most taking it have no 
difficulty passing. 

Some of this success was thought to be the result of special stain automation 
being commonly available. Rene's comment (#1 below) was relevant to the 
observed high pass rate and the awareness that some examinees may have been 
using automation to complete their stains.

The stark reality was that the vast majority of examinees passed the practical 
but a significant percentage were unsuccessful with the written portion of the 
exam. In response to this observation the ASCP in latter years insisted that 
the written exam had to be passed first before the practical could be submitted 
for grading.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, February 20, 2009 11:01 AM
To: Histonet
Subject: RE: [Histonet] Practical Exam

If, indeed, these were the two primary reasons for eliminating the practical 
exam, they are weak and lazy reasons. An increase in the fee to apply would 
cover costs, and, well, was it really that much of a problem of people doing 
other peoples practicals. 

I can't imagine it to be out of proportion to what might (I emphasize might 
and add but likely did not) have occurred sporadically in all the years prior.

I won't pass judgement on a single source, but would love to hear from someone 
who was a part of the decision process that eliminated this practicum.

However, if it is true, my estimation of the ASCP has grossly deteriorated.

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 20, 2009 8:01 AM
To: Victor Tobias; Histonet; Rittman, Barry R
Subject: RE: [Histonet] Practical Exam

There were 2 fundamental reasons why ASCP eliminated the practical part of the 
examination:
1- they got to the conclusion that there was no way to determine if the person 
sending the slides was the one who really made them, and
2- it was getting too costly to send the slides to review or to gather the 
reviewers to qualify the sections, so they decided to eliminate the practical 
and made the changes we have now (renewal and CEU).
René J.

--- On Thu, 2/19/09, Rittman, Barry R barry.r.ritt...@uth.tmc.edu wrote:

From: Rittman, Barry R barry.r.ritt...@uth.tmc.edu
Subject: RE: [Histonet] Practical Exam
To: Victor Tobias vic...@pathology.washington.edu, Histonet 
Histonet@lists.utsouthwestern.edu
Date: Thursday, February 19, 2009, 6:30 PM

Victor
I cannot believe that you have said this.
Although I did not think that the practical examination was the ultimate test 
of skill ,  it did at least provide some uniformity.
With an extension of the logic that you use it is just as easy to allow the 
pathologist to certify that the technician is qualified even without a written 
examination. 
Without a somewhat standardized practical there is no guarantee that the 
technician will have any practical knowledge outside their individual 
laboratory.
Didactic without adequate practical knowledge is, as far as I am concerned, 
useless.
What is really needed is a national standardized written and practical test 
that is administered by NSH.
I am not holding my breath that this will happen.
Barry 


From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victor Tobias 
[vic...@pathology.washington.edu]
Sent: Thursday, February 19, 2009 5:03 PM
To: Histonet
Subject: [Histonet] Practical Exam

There has been discussion regarding the removal of the practical exam.
To me it has not been removed, but the responsibility has shifted to whomever 
signs off on the student. In the case of OJT, the pathologist has verified that 
this student can cut and stain. Of course what is acceptable to one pathologist 
may not be to another. Do they get tested in the art of troubleshooting.. 
As far as the schools go, they shouldn't be graduating 

[Histonet] LabVision/Dako Autostainer

2009-01-23 Thread Della Speranza, Vinnie
We have earlier generation Dako Autostainers and are evaluating a current 
generation LabVision stainer. New or old we have experienced occasions when 
this instrument design has failed to dispense reagent. This seems to occur 
randomly or if there is a pattern we haven't figured it out. I've recently 
learned that some owners of the Biocare Nemesis which is essentially the same 
instrument have also encountered this problem.

The arm will travel to the correct slide but nothing dispenses from the probe, 
then the arm moves on its way. 

I'd appreciate learning if others have experienced this problem and if a 
solution to the problem was discovered I hope you'll share it with me.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 


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RE: [Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ?

2008-11-25 Thread Della Speranza, Vinnie
I'm overwhelmed at the huge response. Thank you all.
The patient was instructed to make arrangements with a funeral home. The 
patient is 29 yrs old and since the funeral home might be taking on the 
responsibility of storing for several decades, she's been unsuccessful in 
identifying one willing to assist her.

I don't have any way to know what the mom's appendix is in. it's quite possible 
she obtained it before the regs on formaldehyde became so restrictive (mid- 
'80's I believe).

The vinegar was thought to be a solution that would not grow organisms. The 
gall bladder has been cut down and now consists of a few very thin strips so I 
was concerned that allowing the tissue to air dry may appear (to the patient) 
that the specimen had been compromised.

Have a great holiday everyone, and thank you again.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: Weber, Susan (VHACLE) [mailto:[EMAIL PROTECTED] 
Sent: Tuesday, November 25, 2008 9:07 AM
To: Della Speranza, Vinnie; histonet
Subject: RE: [Histonet] as Thanksgiving approaches, what do you think about a 
gall bladder in vinegar ?

Does she already have a funeral home picked out? Perhaps she can ask the
funeral home to store it for her, and then release it only to a
funeral home. I would consult my legal department to see what they feel
is appropriate, that way you are dotting all your t's and crossing your
eyes . as well!

Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Della
Speranza, Vinnie
Sent: Monday, November 24, 2008 5:37 PM
To: histonet
Subject: [Histonet] as Thanksgiving approaches, what do you think about
a gall bladder in vinegar ?

I have a patient requesting her gall bladder be returned to her for
religious reasons.
The premise I've been given is so that, upon death, the patient may be
stored with her body parts.

My facility has concerns about providing it to her in formalin (for
obvious reasons) or alcohol. The patient admits this is a family
practice with momma's appendix already being stored in the attic.

It can get a bit toasty warm here in the South so attic storage of a
specimen in alcohol may not be prudent and I can't be absolutely certain
it wouldn't burn the house down, another potential liability for my
institution.

I'm tempted to give it to her in food grade vinegar, to avoid the
potential liabilities from using anything that could be considered
hazardous.
Assuming that returning her gall bladder is a given, what do you think
of using vinegar for this purpose?



Vinnie Della Speranza

Manager for Anatomic Pathology Services

Medical University of South Carolina

165 Ashley Avenue  Suite 309

Charleston, South Carolina 29425

Tel: (843) 792-6353

Fax: (843) 792-8974

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[Histonet] RE: Re: As Thanksgiving Approaches

2008-11-25 Thread Della Speranza, Vinnie
Tom, I'd normally take this approach but if push came to shove I don't believe 
it would hold up in court. That may depend on the language in your surgical 
consent signed by the patient but that aside, the cost of responding to a 
patient's legal action would be much greater than the small effort it takes to 
render the specimen safer to turn over.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Podawiltz, Thomas
Sent: Tuesday, November 25, 2008 9:51 AM
To: Judy Collins; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Re: As Thanksgiving Approaches

Once specimens arrive at our lab, they are ours. We do not give anything back 
to the patient. No legal liability that way.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Judy Collins [EMAIL 
PROTECTED]
Sent: Tuesday, November 25, 2008 9:37 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: As Thanksgiving Approaches

One thing to keep in mind about Vinegar, however, is that fungus will still 
grow in it after a while.  Kind of gross!

Judy Collins
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RE: [Histonet] Re: As Thanksgiving Approaches

2008-11-25 Thread Della Speranza, Vinnie
Patient is listed in our system as Presbyterian.
I'm guessing that patients know that if they cite religion as the basis for 
their request they are less likely to be denied.

I like the mineral oil and glycerin suggestions as they are probably the least 
problematic from a safety perspective.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Robert Richmond
Sent: Tuesday, November 25, 2008 4:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: As Thanksgiving Approaches

I'd think that vésicule biliaire vinaigrette would be inclined to get
the moldies. Of several not very satisfactory solutions, Tony
Henwood's suggestion of mineral oil (paraffin oil) might be the
safest, though messy if it gets spilled.

What religion requires decades-long preservation of gallbladders?
Highly observant Jews sometimes request return of tissues, but their
requirement is that the tissue be buried in a Jewish cemetery - as
soon as possible, not waiting for the rest of the patient to arrive.
Do Muslims have any issues here? - I'm not aware of any Christian
tradition that has any rules about this problem.

In my personal experience, the most common problem of this sort has
been the patient who wants an amputated leg buried with him. Whenever
I've dealt with this problem, a funeral director has bailed me out. As
far as I know, there was no religious issue with the legs, just
personal (or cultural) preference.

The most bizarre situation of this sort happened to me about ten years
ago. A rural midwife had asked an OB-GYN to remove a retained placenta
after a difficult delivery. The OB-GYN put the placenta in formalin
and sent it to a pathology service some distance away. The midwife
called the lab, and was furious to learn that the placenta had been
put in formalin. It seems that (I hope you're not reading your e-mail
with lunch) the midwife had her patients eat their babies' placentas.

I think the JCAHO or somebody banned returning gallstones to patients,
a practice that used to be quite a nuisance for pathologists.

Bob Richmond
Samurai Pathologist
Knoxville TN

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[Histonet] as Thanksgiving approaches, what do you think about a gall bladder in vinegar ?

2008-11-24 Thread Della Speranza, Vinnie
I have a patient requesting her gall bladder be returned to her for religious 
reasons.
The premise I've been given is so that, upon death, the patient may be stored 
with her body parts.

My facility has concerns about providing it to her in formalin (for obvious 
reasons) or alcohol. The patient admits this is a family practice with momma's 
appendix already being stored in the attic.

It can get a bit toasty warm here in the South so attic storage of a specimen 
in alcohol may not be prudent and I can't be absolutely certain it wouldn't 
burn the house down, another potential liability for my institution.

I'm tempted to give it to her in food grade vinegar, to avoid the potential 
liabilities from using anything that could be considered hazardous.
Assuming that returning her gall bladder is a given, what do you think of using 
vinegar for this purpose?



Vinnie Della Speranza

Manager for Anatomic Pathology Services

Medical University of South Carolina

165 Ashley Avenue  Suite 309

Charleston, South Carolina 29425

Tel: (843) 792-6353

Fax: (843) 792-8974

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RE: [Histonet] ASCP HT maintenance fees

2008-11-03 Thread Della Speranza, Vinnie
I want to make you aware that JCAHO requires accredited facilities to undertake 
primary source verification for anyone claiming to have professional 
credentials. If I wanted to hire an applicant claiming to have histology 
certification it is my responsibility as manager to verify with ASCP that the 
individual does in fact have the certification. If you allowed your 
certification to lapse, it would become apparent when I contacted them about 
you and it would end any hope you had of obtaining a position with us.

Only you can decide if the costs to maintain your certification are worth it 
but I thought you should be aware of the process that is followed to verify 
credentials. I agree with Tom that indicating that you allowed your 
certification to lapse would reflect negatively and likely sabotage hopes of 
employment, at least in some environments



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Podawiltz, Thomas
Sent: Monday, November 03, 2008 3:07 PM
To: Kimberly Tuttle; R C; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] ASCP HT maintenance fees

If you were certified in 2004 or after you need to turn in 36 credit hours of 
continuing education in order to maintain your certification. You do not need 
to be a member of ASCP to be certified, however you do get some free CE hours 
with your membership. My certification was in 85, so yes, I am one of the old 
farts that is exempt. However, I have stayed current with my education. even in 
the years that I did not practice Histology.

As a supervisor, I would not look at a resume that had an expired 
certification. Right or wrong I would assume that, the applicant did not take 
this field seriously enough by letting their certification lapse.

Tom Podawiltz, HT (ASCP)
Histology Section Head/Laboratory Safety Officer
LRGHealthcare
603-524-3211 ext: 3220

From: [EMAIL PROTECTED] [EMAIL PROTECTED] On Behalf Of Kimberly Tuttle [EMAIL 
PROTECTED]
Sent: Monday, November 03, 2008 2:08 PM
To: R C; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] ASCP HT maintenance fees

Really? I never pay to maintain HT certification. As far as I know theres a 
ASCP membership fee, but you dont have to be a member to be certified. Am I 
wrong here?

Kimberly C. Tuttle  HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax


 R C [EMAIL PROTECTED] 11/3/2008 12:49 pm 
Can someone assist me in rationalize the annual cost of maintining HT
certification (roughly $100 annually) and its benefit? Point accumulation is
generally low for classes you must pay for, and those who obtained
certification prior to 2004 are exempt. Should one not pay the annual fee,
certification is dropped Is this correct?). In that case, can one advertise
HT certification for future employment opportunities then, offer full
explanation (and expired certification) during interview and that be
sufficient?

What I generally receive from ASCP is an annual bill and a random newsletter
from time to time. Furthermore, when a bill isn't paid on time,
the termingology in the subsequent bills become similar to that of a
collection agency. Frankly, I find this mailing submission as well as state
and national meetings more informative.

Someone please clarify something I might be missing and any benefits of the
pay out.
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RE: [Histonet] bone marrow biopsies

2008-10-30 Thread Della Speranza, Vinnie
You are being placed in a difficult position that will only translate into 
physician unhappiness, so even though they are in a hurry (and usually for 
valid clinical reasons) the fact is that compromises will affect something else.

I'm assuming you are a one shift operation. My lab is 24 hours, which allows us 
sufficient time for fixation and decalcification while enabling slides to be 
out the next morning.

While Dr. Richmond is correct that there are faster decalcifiers, I'm guessing 
they want immunohistochemistry in at least some cases and the mineral acids 
will destroy your hopes of good immuno staining. We use 10% formic acid and 
still have to decalcify for about six hours in order to have blocks that 
section well.

If you had an evening shift I'd suggest shortening your processing time. 
Shortening fixation time is counter productive to good morphology and good IHC.

Lastly, you don't indicate if your bone marrows arrive at all times of the day. 
If you are trying to complete fixation and decalcification on specimens 
arriving in the afternoon, all to be on the processor at the end of your shift, 
 you end up in the position you find yourself currently, with blocks poorly 
decalcified that section poorly.

The only way to make this work to everyone's satisfaction is to have a longer 
work day, if it is possible to stagger work shifts. This also presumes that you 
have multiple tissue processors and can place your bone marrows on a shorter 
cycle, perhaps along with other biopsies.

Others here may be able to advise you regarding using microwave technology for 
fixation and decalcification which may ultimately save the day for you.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974
 

-Original Message-
From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Cynthia Robinson
Sent: Thursday, October 30, 2008 11:44 AM
To: histonet
Subject: [Histonet] bone marrow biopsies

We are currently using 10% formalin fixation on our bone marrow cores.  We fix 
for 2 hours minimum prior to decal.  We are using Immunocal from Decal Corp. 
for 2-4 hrs followed with processing overnight in VIP.  Cores are still crunchy 
upon sectioning and we are doing surface decal for up to 30 min. Our paths want 
cores turned out within 24 hrs following procedure. Any suggestions? 
 
Thanks.
Cindi 
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[Histonet] NSH Blog

2008-09-11 Thread Della Speranza, Vinnie
I didn't see this announcement posted on the list and thought it might be of 
interest to some.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue  Suite 309
Charleston, South Carolina 29425
Tel: (843) 792-6353
Fax: (843) 792-8974

FYI


BLOGGING NSH




The National Society for Histotechnology's Annual Symposium/Convention is one 
of the preeminent events for histology and pathology laboratory administrators 
and professionals.  Each year it continues to grow in popularity and attendance 
as NSH members and people from all facets of the histotechnology industry come 
together to share ideas and get a first-hand look at the new and exciting 
technologies that are helping labs work smarter and become more effective.

This year it's being held at the David Lawerence Convention Center in 
Pittsburgh, PA on Sept 12 - 18. General Data will be showing our ID/Positive 
line of bar code specimen identification solutions (we're at booth 506  508).  
If you're coming to the show, please stop by our booth and say hi.

If you can't make it to the event, stay tuned to this blog.  In addition to 
exhibiting, we'll be blogging the show. During (and after) the show, we'll be 
posting pictures, video interviews, and other information on what we find at 
the NSH Convention - what's new, what's hot, what's on attendees' minds.

Our intention is for this blog to be the eyes and ears at NSH for the entire 
histo community, particularly for those who can't attend the event.

Please pass this on to anyone who you feel might be interested in following our 
posts from the NSH Convention.

And if you have any comments or suggestions regarding topics or other subject 
matter from NSH you would like us to cover, just let us know by e-mail or in 
the comments section.


Tags :

posted By : Ralph Moher on 9/8/2008 12:31:18 PM in

http://www.general-data.com/Blogs/post.aspx?id=35




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