I am not sure what others do, but I do not run my Azure B slides through
alcohol because I find the alcohol diminishes the intensity of the green.
I air dry, clear, then coverslip.
Diane Craft
Pathology Department
Animal Medical Center
510 East 62nd St
New York NY 10065-8314
212-329-8675 (phone)
212-759-5878 (fax)
Steve Olsen sol...@mplnet.com
Sent by: histonet-boun...@lists.utsouthwestern.edu
08/17/2011 02:56 PM
To
Margaryan, Naira nmargar...@childrensmemorial.org,
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,
histonet-requ...@lists.utsouthwestern.edu
histonet-requ...@lists.utsouthwestern.edu
cc
Subject
[Histonet] RE: melanin counterstaining
You can try a simple Schmorl's stain - which stains melanin. You can find
it in a Sheehan/Shrapchek
Steven K. Olsen
Chief Operating Officer, Director of Anatomic Pathology
Molecular Pathology Laboratory Network, Inc.
250 E. Broadway, Maryville, TN 37804
P 865.380.9746 ext.187
C 865.978.0321
F 865.380.9191
http://www.MPLNET.com/
http://www.Geneuity.com/
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan,
Naira
Sent: Wednesday, August 17, 2011 2:51 PM
To: histonet@lists.utsouthwestern.edu;
histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] melanin counterstaining
Good morning,
I have heavily pigmented melanocytic cells and trying to differentiate
between melanin pigment and chromogen (DAB) by using Azure B.
After IHC with DAB I incubate slides for 30-45 min in the working solution
of:
* 4ml of a 25mg/ml aqueous solution of Azure B
* 3.4ml of 0.1M acetic acid
* 600µl of 0.1M sodium acetate
* 27ml of DI water
* 5ml acetone
Then water wash and hematoxylin counter-stain. By the end all melanin
supposed to turn blue-green, but I still see some remained brown melanin.
My question is: I would like to increase green color, so the concentration
of what reagent I have to increase?
Thanks in advance,
Naira
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