Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades
Michael Dietrich and his team at Southeast Pathology Instrument Service is outstanding! www.southeastpathology.com Thanks, Drew Meyer, HT Anatomic Pathology Independent Contractor On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Can anyone suggest a good company that works on old microtomes? Ours is > not broken, it's just in need of some TLC. Also, what is a reasonable > price for such work (we have a RJ 2040)? > > Also, we have a lot of the old metal blades that you have to sharpen. Any > suggestions on how to sell, donate, recycle such items? Do people even use > these anymore? I would hate to have them thrown out. > > Thanks! > Michelle (Shelly) Aono > ~~~ > Research Associate II > 107B/124 Greene Hall > Auburn University, Dept of APP > Auburn, AL 36849 > (334) 844-5594 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 2014 IHC CPT coding
This is from the CAP site... and here's the link: http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=/portlets/contentViewer/show_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=statline/index.html_state=maximized_pageLabel=cntvwr *Immunohistochemistry: 88342 (PC TC)* CMS rejected the CAP’s proposal and instead will require the use of two new G codes for this service, including G0461 to report one unit of service per specimen and G0462 to report each additional stain. The following chart documents the percentage reduction and payment rate change for the new G codes compared to the current reimbursement for CPT code 88342: *CPT Code**Modifier* *88342 2013 Total Payment**2014 Total Payment* *Total % Change from 2013 88342* G0461 $115.34 $88.04-24% G0461TC$73.15 $57.39-22%G0461 26$42.19 $30.65-27% G0462 $115.34 $68.08-41% G0462TC$73.15 $55.61-24% G0462 26$42.19 $12.48-70% The changes occurred under the Affordable Care Act (ACA), which provided CMS expanded authority to launch its “misvalued code” initiative. CMS targeted the top expenditure codes from each specialty as potentially overvalued. This action triggered the review of the three additional high volume code families. Moving forward, the CAP will work with the AMA and other groups to pressure CMS to change their G code definitions so that payment is made ‘per slide’ rather than ‘per specimen.’ On Tue, Dec 10, 2013 at 5:22 PM, Kim Donadio one_angel_sec...@yahoo.comwrote: I got information they got rid of 88342 all together and added G codes. One for the 1st slide stained and another for each additional stain. Can anyone verify and give a link? Sent from my iPhone On Dec 10, 2013, at 4:33 PM, Cartun, Richard richard.car...@hhchealth.org wrote: My understanding is that 88342 is used for the first IHC antibody, then 88343 is used for each subsequent antibody (per specimen part). Is that correct? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD200
Is anyone out there running CD-200 with good results? If so, I would really appreciate it if you would email me privately so I can ask you a few questions. Thanks, Drew Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] DAKO Autostainer Issues
There's a circuit board on the arm near the barcode scanner that controls the movement and orientation of the arm. If that circuitboard malfunctions, then you can see some of the erratic behavior that you are describing. That's one possibility I can think of that might cause that kind of problem. However, the technician who looked at it should be able to troubleshoot and replace that board if it is a problem. Drew Sent from my iPhone On Mar 12, 2012, at 2:31 PM, friedrich_h...@bd.com wrote: We have been having issues with our Autostainer since bringing it out of storage. All parts are in excellent shape, according to the technician that performed the PM. It worked fine for a month of so, but then began malfunctioning after only a couple of staining runs. At some point in the staining process, the machine appears to lose its XY homing and jams itself against the front right corner, dispensing buffer onto the floor of the chamber and occasionally making grinding noises. We've had a technician take a look at it, 'clean' the software, and we thought we had it resolved, but the problem appears to be back. Has anyone experienced this issue? If so, was it hardware- or software- based? Thanks in advance! - *** IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygr...@bd.com. *** This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. *** Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Surgipath Millenium VSP3001/Leica Vogel 35396 Slide Writer
So apparently this model of slide writer is no longer manufactured, at least from what I can tell. My Leica rep says they don't make it anymore, but I really need to get my hands on one. Normally, I'm not a big fan of this printer for larger volume labs, but the way we make slides here at our immuno lab is perfectly conducive for the specific design of slide writer. I'm wondering if anyone out there in Histoland knows where I can get one new or used. Also, do you know if Leica still sells the ribbons that go with them? Finally, the ideal circumstance would be where we could demo one here; you know how it is getting the funding approved! However, one of our Pathologists has straight up said he'd buy it himself and worry about getting reimbursed for it later. Anyways, please let me know if you can help or point me in the right direction! Thanks, Drew Meyer, HT CSI Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] H. Pylori - IHC
We use the Rabbit Polyclonal H. pylori from Cell Marque on the Ventana Ultra's... We're a natinowide reference laboratory and our clients are all very happy with the specificty of the staining. Let me know if there's any more information you need. Drew On Wed, Nov 16, 2011 at 17:15, beth@hcahealthcare.com wrote: Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 mailto:beth@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Varicella Zoster
abcam has a few Varicella Zoster strains to choose from... I haven't used them before, so I can't speak of how well they work or provide much insight to dilutions and protocols for the Ventana, but the other antibodies I've used from them work well. Drew Sent from my iPhone On Oct 28, 2011, at 12:11 PM, Donna Liaros dlia...@bioreference.com wrote: Anyone have any recommendations for a vendor that supplies a concentrated antibody for Varicella Zoster Virus (herpes zoster)? I would be optimizing it on the Ventana Ultra. The information transmitted in this email and any of its attachments is intended only for the person or entity to which it is addressed and may contain BioReference Laboratories proprietary information, which is privileged, confidential, or subject to copyright belonging to BioReference Laboratories. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited and may be unlawful. If you received this in error, please contact the sender immediately and delete and destroy the communication and all of the attachments you have received and all copies thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Creative Waste Solutions
I may be wrong about this, but I don't think Rex has anyone else distributing his stuff yet. However, some of the stuff they sell is merely products that they relabel to distribute themselves. So, it depends on what specific products you're talking about. Drew Sent from my iPad On Aug 17, 2011, at 9:42 AM, Anita Buchiane abuchi...@bmhvt.org wrote: Does anyone know of a distributor of Creative Waste Solutions products? ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark Ultra-Red Problems
Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, Susan Foreman sfore...@labpath.com wrote: We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from purified to distilled. Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark Ultra-Red Problems
Is it appearing on every slide run with that detection regardless of the antibody? What are you using to remove the liquid coverslip? If it's dish-soap, did you just recently change brands or use one with a different formula? Drew Sent from my iPhone On Aug 1, 2011, at 12:24 PM, Susan Foreman sfore...@labpath.com wrote: We have tried both lots of the Ultra View Red -Original Message- From: Drew Meyer [mailto:41dm...@gmail.com] Sent: Monday, August 01, 2011 12:27 PM To: Susan Foreman Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Benchmark Ultra-Red Problems Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, Susan Foreman sfore...@labpath.com wrote: We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from purified to distilled. Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] special stain controls
I can provide you with all the H. pylori you could ever want if you have some good fungus blocks. Let me know if you'd like to trade! Drew Sent from my iPhone On Apr 29, 2011, at 6:20 PM, histot...@imagesbyhopper.com wrote: Hi, I am in need of control blocks for special stains, H. Pylori, pneumocystis and AFB. I am attempting to grow our own AFB, but am having difficulty getting the other control blocks. Commercial vendors charge a LOT of money for these controls! I was hoping to find a way to swap controls, if I could. Does anyone have any suggestions for me? I don't mind buying them, but tend to cringe when the costs are around $10/slide!! Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sunquest CoPath Rep
Could any Sunquest CoPath sales rep please contact me privately off the list? Thanks, Drew Meyer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing question
Hey Joyce, My understanding has always been that if the pathologist specifically mentions the stain in the report, then it's OK to charge all the fees. However, if the pathologist chooses to leave any mention of the stain/immuno in the report, then you can't bill for it. So if the pathologist mentions a specific stain was done, but that the findings were negative, you can still charge. In the case of an exhausted tumor, I think the ultimate discretion comes to whether or not the pathologist chooses to mention it in the report. That's just from my experience, but I would love to hear how others are handling instances such as these, too! Drew On Thu, Jan 6, 2011 at 12:22, Weems, Joyce jwe...@sjha.org wrote: When you have performed a special stain or immuno that does not help the pathologist (tumor exhausted, etc) do you still charge for the technical component and the pathologist credits the professional fee? Just curious.. Happy New Year, Everyone! Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP 1000 Help
We've got the old workhorse, the VIP 1000, that we still use. During a move, the guide, which shows all the commands you can do with that magnet, got lost. Is there anyone out there that has this and could send or fax me a copy? It would be much appreciated! Thanks, Drew Meyer -- ~Love is like a booger. You keep picking at it until you get it, then wonder what to do with it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil Red O control
There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna anna.in...@stmarygj.org wrote: Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] hi-profile blades
I agree with Bernice... I've used both the low and high profile Sakura AccuEdge nothing comes close to their quality and consistency. Drew On Tue, Oct 19, 2010 at 11:44, Bernice Frederick b-freder...@northwestern.edu wrote: I've been using Accu-edge blades from Sakura and have found nothing to equal them. We use low profile here, but high are the same quality. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Tuesday, October 19, 2010 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hi-profile blades Hi All, One of my techs loves using hi-profile blades but recently she has been having problems with the quality and consistency of the blades. One will cut great and the next one will give her problems cutting. I'd appreciate any suggestions for blade type/company that histotechs are happy with so we can try them out. Thanks, Michele Margiotta BMHMC Histology Supervisor 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question about Oil Red O controls
Just use Mayo (not fat free, of course!) and smear it on the slide like you would a blood smear. It stains beautifully. Drew On Wed, Oct 6, 2010 at 09:15, Komal Gada kjg...@gmail.com wrote: Hello Histonetters, I am trying to find a procedure for using butter and egg yolks as controls for the Oil Red O stain (to show the fat). Does anyone have something they would be able to share with me? Thanks, Komal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Precipitate in Processor
My guess is that either your 70% wasn't made up properly and was a higher concentration or it's been so long since you've changed the solution that the water is fully saturated with the formalin salts. If it becomes a regular problem, you might consider reducing your first alcohol's concentration to 60% or even 50%. Good luck! Drew On Mon, Aug 9, 2010 at 12:00, Adrienne Aperghis Kavanagh aaperg...@uspath.com wrote: Hello Everyone, Has anyone ever seen a (salt?) precipitate in their alcohols following formalin? While changing the processor this morning, I noticed a precipitate in the 80% alcohol and 95% alcohol (NOT in the 70% alcohol). It is white and grainy. The alcohols were otherwise unaffected. We are using a 10% NBF containing: Formaldehyde Water Sodium Phosphate, monobasic Sodium Phosphate, dibasic Methanol And our alcohols are all reagent grade. Any help would be very much appreciated! Thank you in advance! Adrienne Aperghis Kavanagh US PATH 30 W. Century Road Suite 255 Paramus NJ 07652 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding multiple GI pieces on end in a paraffin block
We regularly embed 6-12 pieces on end in one block without any special method. You just have to be quick... and don't leave the block on the cold plate very long... just touch the cold plate briefly while embedding the individual piece, then lift the block off the plate until you grab the next piece... repeat quickly and you'll run out of room in the mold before you'll have to worry about it hardening too much. Drew On Fri, Jul 16, 2010 at 11:23, kgrob...@rci.rutgers.edu wrote: Is there a way to do this without one or more pieces falling over? I saw in the archive the method for frozen sections-embed them on their sides in OCT, then cut on the end, but I don't think I'd be able to do that in paraffin. Would one of the tissue microarray methods work? (I've never done that before, so I have no idea.) Thanks in advance for all your help, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VIP 2000
You can always call Sakura... they'll at least tell you what the codes means... Drew On Fri, Jul 9, 2010 at 10:15, sgoe...@xbiotech.com wrote: Hello all, I recently purchased a refurbished VIP. ; This would have been the 3rd time I have run it, but wait...diagnose flas hes and I get an error code 73. Of course the manual doesn't say what this is or how to fix it, and I can't seem to find anything online either. I know this is an older machine and lots of you have used it, was ju st wondering if anyone knew what the heck diagnose code 73 is? Th anks Sarah Goebel, B.A., HT (ASCP) /div Histotechnician XBiotech USA Inc. 8201 E ast Riverside Dr. Bldg 4 Suite 100 iA ustin, Texas 78744 (512)386-51 07 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] used histology equipment
Absolutely... Southeast Pathology Instrument Service out of Charleston, SC. The owner's name is Michael Dietrich. I've done business with him before and they are great people, very honest and they stand behind their instruments. I would highly recommend them to anyone. Contact Michael directly and tell him Drew Meyer from Atlanta referred you. http://southeastpathology.com/ Drew On Tue, Jul 6, 2010 at 18:29, dcoj...@tampabay.rr.com wrote: Does anyone know of a reputable dealer for used equipment? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori RTU antibody
I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: H. pylori RTU antibody
I'm getting tons of responses from people about other antibodies that work well, which I appreciate very much... However, I'm curious to hear from someone that's actually using the Dako RTU and having success... Thanks, Drew On Wed, Jun 30, 2010 at 10:41, Drew Meyer 41dm...@gmail.com wrote: I was just wondering if a lot of people use the RTU antibodies available out there for H. pylori. Currently, we're testing the RTU from Dako and we're having issues with a lot of background staining. After tweaking the protocol a lot, I've managed to get a lot of it removed, but there are still some issues. I was wondering if most people are making their own dilutions to get a better stain or if the RTUs are commonplace. I appreciate the feedback! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Trichrome Help
I'm trying to troubleshoot a problem we had today with a Trichrome stain and I was wondering if anyone out there could help. We do Gomori Blue Collagen Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in the Gomori Blue Collagen stain followed by a quick change through acetic acid, alcohol and xylene. When it's all done, there is this weird film that is present over the entire surface of the slide... almost like the thin gray film that you sometimes see when you leave slides too long in the silver solution for a GMS. Anyways, I'm not sure what's causing it and putting the slide in xylene or alcohol for a long time doesn't get rid of it. It can easily be wiped off, but obviously we can't wipe the section off. If anyone could help me narrow down what step might be causing this, I'd appreciate it! Thanks, Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amyloid Stain
Hey Guys, I was hoping to get some help with the best way to approach a request for an amyloid stain. The only thing I've ever done in the past is the traditional Congo Red... however we were wondering how the Amyloid A Immuno stain is. Our pathologists were wondering if the Amyloid A is more/less reliable and if there are any limitations associated with this antibody. I just don't know that much about it and was hoping you guys could enlighten me! Thanks for the help! Drew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] help
So sorry... the only thing I know to fix it is time... about a week or
two... :)
Drew
On Tue, Mar 23, 2010 at 11:59, Patsy Ruegg pru...@ihctech.net wrote:
After you stop laughing seriously I need some help here, apparently I got
my
fingers in some silver nitrate yesterday and touched my face under my nose
over my lip and now I have black spots that won't come off. I have done
this on my hands before but never on my face. So far I have tried soaking
a
cloth in hydrogen peroxide hoping to bleach it with no luck, I even tried
putting some gold chloride on it to see if I could tone it down, to no
avail. Any ideas? Make up only goes so far and lasts so long.
Regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pru...@ihctech.net
web site www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
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presented are solely those of the author. It may contain information that
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any dissemination, distribution, copying, or other use of this message, or
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Re: [Histonet] glass coverslipper
We use the Tissue-Tek Glas with great results. Knowing how Sakura loves to just relabel Leica equipment, it might be the same as the CV5030, but I'm not sure. Either way, I've been very impressed with the Tissue-Tek machine. Drew On Thu, Mar 4, 2010 at 15:00, Derek Papalegis derek.papale...@tufts.eduwrote: Hi Everyone, I am looking for a glass coverslipper and was wondering what people recommend. I am in a small research lab so having a small footprint is very important. It doesn't have to be very fast but I need something dependable that will give consistent results. Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] time tissue stays in paraffin
What is the make/model of your tissue processor? Drew Sent from my iPhone On Dec 10, 2009, at 10:56 AM, Dunlap, Charles dunl...@umkc.edu wrote: We have a software glitch in our tissue processor, it stops in the first paraffin and will not progress to paraffins 2 and 3 then complete the cycle. If we catch it quickly, we can instruct it to continue but the cycle happens around 5:00 am so we sometimes are not here, tissuet may spend an extra 30 minutes or even an hour in the first paraffin. Estimated cost of repairing is a whopping 6 thousand $$.Does this have a major effect on quality of slidessometimes ours are less than ideal but I am not sure if the problem is in the paraffin cycle or elsewhere...sectioning, staining, etc. Any ideas? Charles Dunlap ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paper on osteoclasts
Is this it? http://www.jbmronline.org/doi/full/10.1359/jbmr.2000.15.8.1477?cookieSet=1 I've never read the paper, so I don't know exactly what you're looking for... but I sometimes find Google Scholar to be a better search engine for journal articles (which is what I used to find the above article). Drew On Tue, Dec 8, 2009 at 01:36, louise renton louise.ren...@gmail.com wrote: Hi all, I am going crazy - and its not even friday yet! A little while ago (OK about 3 years) i came across a paper where the researcher had shown the transition of macrophages to osteoclasts using immuno staining - i cannot seem to find it on Pubmed, dont know the author date or journal. i hope soemone out there can help best egards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP question
There has already been a lot of responses to this, but I thought I'd add mine anyway. For all our specimens in-house that are delivered from surgery, we have three identifiers present: name, DOB and MR number. We eliminated the use of SSN (from our reports as well), since we all know how dangerous it is to have your SSN just floating around. Specimens received from outside facilities are a little different, because the outside facility can't possibly label the specimen with our MR number; it's assigned once the specimen arrives at our location. In those instances, just the name and DOB are used. At the time of accession, we do add the in-house pathology case number to all the specimens as well. You might call this overkill, but it helps greatly when we need to pull a specimen later on and it helps cut down on grossing errors. The big thing from CAP is the two identifiers. When you're specifically dealing with outpatient centers where you might not be able to get the medical record number on the container at the time of collection, it seems to me that the best choice is to use name and DOB. Just my 2 cents. Drew On Wed, Dec 2, 2009 at 11:49, Una McGiven lost.dragon...@yahoo.com wrote: Hi everyone, On the most recent CAP checklist there is a requirement for 2 patient identifiers. For those labs who are CAP and receive all or a large portion of their cases from outside facilities (=not from your facility), what are you using as the identifiers on your specimen containers? Name and birthdate? SS#? Med record? Something else? Thank you in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I'd be curious to see how you have your processor times set for each station. I'm sure you're using a different setting on the weekends to account for the longer time. Have you made sure that all the other stations are getting the same time they normally get during the week? Drew On Wed, Nov 25, 2009 at 10:13, Taylor, Robin robin.tay...@prexushealth.com wrote: We have been having processing problems for a while now and cannot resolve it. It only happens on weekends. When we embed and cut on Monday morning, alot of our larger pieces of tissue, especially breast, are not fixed and processed well. I would expect the opposite especially since they are in formalin for an additional 48 hours. We do not work Saturdays (sorry guys) so the tissue is grossed and submitted on Friday. Does anyone have any ideas as to why this would be and what I can do to fix it. Thanks so much. Robin Taylor HT Butler County Medical Center Hamilton, OH This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CMS/NCCI Update Dated October 1, 2009
Joyce, That's very interesting, as I remember going to great lengths a few years ago to be sure we had a billing limiter in place in CoPath to prevent the multiple charging of CPT codes on the same specimen. However, I looked up the document released on 10/1/09 and here's what I found in regards to the special and immuno CPT codes: 8. The unit of service for special stains (CPT codes 88312-88313) and immunohistochemistry (CPT codes 88342, 88360, 88361) is each stain. If it is medically reasonable and necessary to perform the same stain on more than one specimen or more than one block of tissue from the same specimen, additional units of service may be reported for the additional specimen(s) or block(s). Physicians should not report more than one unit of service for a stain performed on a single tissue block. For example it is common practice to cut multiple levels from a tissue block and stain each level with the same stain. The multiple levels from the same block of tissue stained with the same stain should not be reported as additional units of service. Only one unit of service should be reported for the stain on multiple levels from the single tissue block. Additionally, controls performed with special stains should not be reported as separate units of service for the stain. From what all I can see, you're right; it seems that we can bill for multiple special stains on the same specimen, just not the same block (so tell Drs. Stargel and Sears it won't help them :) ). I'm going to present this to our pathologists and billing auditors for review. Thanks so much for sharing this with everyone!!! Drew On Fri, Nov 20, 2009 at 14:28, Weems, Joyce jwe...@sjha.org wrote: According the one of our pathologist the above mentioned update clarified that CMS allows the billing of special stains performed on different blocks of the same Pathology specimen. Previously, it was understood that a special stain could only be billed once per specimen. Now it appears that a stain could be billed twice if performed on block 1 and block 3 of the same specimen. Has anyone else reached this conclusion? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] B5 alternate
Essentially it's a zinc formalin. Drew Sent from my iPhone On Nov 16, 2009, at 4:53 PM, John Kiernan jkier...@uwo.ca wrote: What is this B plus fixative? Is it a trade-secret brew? John Kiernan Anatomy, UWO London, Canada = = = - Original Message - From: Anne van Binsbergen anni...@gmail.com Date: Friday, November 13, 2009 10:01 Subject: Re: [Histonet] B5 alternate To: Drew Meyer 41dm...@gmail.com Cc: Knutson, Deanne dknut...@primecare.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu B plus is the one - we use it as stated and it works like a charm immunos are good too - we are proudly mercury free Annie 2009/11/13 Drew Meyer 41dm...@gmail.com In my lab, we're using B-Plus Fixative by BBC Biochemical. I know many other labs that are also using it as their B5 alternative. The biggest pro, of course, is that it's mercury free and not hazardous. Also, it's not as sensitive to over-fixation like B5 is. The guideline I use is 4 hours minimum fixation for Bone Marrows and Lymphoid tissue. After that, routine formalin processing is all you need to do. You can, however, leave the specimen in B- Plus for up to 48 hours without adversely effecting the tissue sample. Hope this help. Drew Meyer On Fri, Nov 13, 2009 at 09:43, Knutson, Deanne dknut...@primecare.org wrote: Am looking for advice on what alternate fixative to use to replace B5. What do the majority of you histonetters use? Does it work well with immunos? The pros and cons? Thank you for your recommendations. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, North Dakota 58506 (701)-530-6730 dknut...@primecare.org mailto:dknut...@primecare.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Anne van Binsbergen (Hope) Abu Dhabi UAE ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
