[Histonet] slide box freezer racks
Hi I have a gazillion Fisher brand slide boxes that hold 50 slides each. We store the slides (fresh frozen unfixed) at -80 in freezer bags, but they are just piled into the freezer. Does anyone know of a source for a rack to organize such boxes in a -80 chest freezer? Thanks, Ed Roy -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] motorized cryostat
I have a small research lab with an old (very old) Minot IEC microtome cryostat, which is on its last legs. In starting to shop for a new one, I was wondering how much difference a motorized one would make. We do mostly fresh frozen (unfixed) mouse brain and lymph node sections. Are the sections any better? Thanks for any opinions on this and any recommendations on a good cryostat at a reasonable cost. Ed Roy -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lymph nodes peeling out of OCT
Hi We do a lot of IHC on mouse lymph nodes, unfixed, fresh-frozen in OCT. Sometimes individual lymph nodes will peel away from the OCT as they are being cut, and then curl up, making them impossible to get a good section. In the same mold several will be fine and one will curl up. Cutting further on the same block, the problem lymph node may be fine. Any ideas on what variables determine this, either in the initial freezing, or in the cutting? Would it help to soak the tissue at room temp in the OCT for a period of time before freezing? Dip in PBS before freezing? Thanks for any insights. Ed -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lymph nodes peeling out of OCT
Hi We do a lot of IHC on mouse lymph nodes, unfixed, fresh-frozen in OCT. Sometimes individual lymph nodes will peel away from the OCT as they are being cut, and then curl up, making it impossible to get a good section. In the same mold several will be fine and one will curl up. Cutting further on the same block, the problem lymph node may be fine. Any ideas on what variables determine this, either in the initial freezing, or in the cutting? Would it help to soak the tissue at room temp in the OCT for a period of time before freezing? Dip in PBS before freezing? Thanks for any insights. Ed -- Edward Roy, PhD Professor of Pathology Professor of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign 506 S. Mathews Ave. Urbana, IL 61801 217 333-3375 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
