[Histonet] Lab equipment
Hello Donielle, We are interested in the IHC stainer. I assume this is in working condition? Is the software(License) included also? Please PM me at this email address. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 118 Main St, Store Front, Suite 1 Webster, MA 01570 Lab - 508-461-7207 www.histologistics.com http://jmdhistology.com/ -To Err is Human--- The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Thu, Mar 11, 2021 at 1:08 PM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. Re: formalin disposal (Donielle Hunter) >2. Lab equipment (Donielle Hunter) > > > -- > > Message: 1 > Date: Thu, 11 Mar 2021 09:24:01 -0500 > From: Donielle Hunter > To: "Townsend, Suzanne" > Cc: "histonet@lists.utsouthwestern.edu" > > Subject: Re: [Histonet] formalin disposal > Message-ID: > < > cacgo-fb_3h_ws8lw8hsyc2swytd0dbe9hykgkk3ifsooa_y...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello, > We are also a small GI lab and we use HydeAway to neutralize our 10% NBF. > After allowing to sit for the recommended amount of time we discard it down > the drain flushing with plenty of water. > > On Fri, Feb 26, 2021 at 1:25 PM Townsend, Suzanne via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > I am at a small lab where we discard of our own formalin. We use 10% NBF > > and neutralize using Vytac. I had been using a residual aldehyde test > that > > was discontinued. I ordered a new test kit, but am not thrilled... What > do > > you all do to test neutralized formalin prior to disposal? > > > > Thanks, Suzanne > > stowns...@nmda.nmsu.edu > > > > > > > > Confidentiality Notice: New Mexico has a very broad public records law. > > Most written communications to or from state employees are public > records. > > Your e-mail communications may therefore be subject to public disclosure. > > This e-mail, including all attachments is for the sole use of the > intended > > recipients. Any unauthorized review, use, disclosure or distribution is > > prohibited unless specifically provided under the New Mexico Inspection > of > > Public Records Act. > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > > Message: 2 > Date: Thu, 11 Mar 2021 09:30:27 -0500 > From: Donielle Hunter > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] Lab equipment > Message-ID: > k9bbod87szq...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello all. > > I am trying to replace one of our IHC stainers. I tried seeing if the > original company wanted it back and checking with buyers who refurbish lab > equipment with no success. > > Would anyone be interested in a BioCare Intellepath IHC stainer? It comes > with all of its accessories, extra carboys, tubing, PC, printers and > decloaker for offline antigen retrieval. > > If interested please respond. > > Thank you, > Donielle Hunter > > > -- > > Subject: Digest Footer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > > End of Histonet Digest, Vol 208, Issue 8 > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H Pylori controls
Hello Cynthia, We have HP blocks. Our tissues are great for IHC but not special stains. Please contact me directly if you are interested. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 118 Main St, Store Front, Suite 1 Webster, MA 01570 Lab - 508-461-7207 www.histologistics.com http://jmdhistology.com/ -To Err is Human--- The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Fri, Jan 22, 2021 at 1:12 PM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. H Pylori controls (Cynthia McGrady) >2. Service provider for growing fungal strains? (Craig) >3. Embedding (Ann Specian) >4. water under sectios (Bernice Frederick) > > > -- > > Message: 1 > Date: Thu, 21 Jan 2021 13:21:14 -0500 > From: Cynthia McGrady > To: Histonet > Subject: [Histonet] H Pylori controls > Message-ID: > < > cafn2hshezlsrd-8brzj+8ezknn3zxvoglz93+bgmov820sz...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello Histonetters. > Looking for positive H Pylori control block(s). We typically use > positive case tissue from old surgical cases, but seem to be running low. > If anyone has a surplus, and would be willing to share, or maybe we have > tissue you can use?? We could do a swap..Let me know..Thanks...Cindy > McGrady > > Cindy McGrady > HT(ASCP) QIHC > Hurley Medical Center > One Hurley Plaza > Flint, Mi 48503 > 810-262-9138 > > -- > > > If you are not the intended > recipient(s), you are notified that any > disclosure, copying, distribution or > any action taken or omitted to be > taken in reliance on the contents of this > information is prohibited and may > be unlawful. If you receive this message in > error, or are not the named > recipient(s), please notify the sender, delete this > e-mail from your > computer, and destroy any copies in any form immediately. > > > -- > > Message: 2 > Date: Thu, 21 Jan 2021 12:35:25 -0600 > From: Craig > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Service provider for growing fungal strains? > Message-ID: > 9lyz12axoa67mv0cdfak1v0n5cmxnmfyu90vydfe7...@mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello all! > > Does anyone know a lab that can grow specific fungal strains? > > We are looking to make internal FFPE control blocks for specific fungal > strains. We have an internal method for producing FFPE blocks. Just need a > lab or service provider that can grow specific fungal cultures and send us > the fixed bugs. > > Any suggestions would super grateful! > > Best, > Craig > volle...@gmail.com > cr...@champdx.com > ? > > > -- > > Message: 3 > Date: Fri, 22 Jan 2021 15:43:26 + (UTC) > From: Ann Specian > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] Embedding > Message-ID: <655838156.2928090.1611330206...@mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing > required to perform embedding in NJ?Thank you, Ann > > -- > > Message: 4 > Date: Fri, 22 Jan 2021 17:15:40 + > From: Bernice Frederick > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] water under sectios > Message-ID: > < > mwhpr05mb294386def0662af11f9ff66c8f...@mwhpr05mb2943.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good morning (well, in Chicago anywa
[Histonet] Thermo Cryostar NX
Hello All, We have a distributor who is looking for someone that has a Thermo Cryostar and would be willing to test some specimen disks and give feedback. They are willing to give you disks (Free) after testing. If Interested please contact Ken Marzinsky or Helena Murong at kdea...@hotmail.com, hmur...@springsidesci.com, Phone: 919-864-8821. Thank you Edit Contact Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 www.histologistics.com -To Err is Human--- The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BRCA1 on Benchmark Ultra (Curt)
Hello, We've had good results with Calbiochem antibody Cat#OP92 clone MS110, dilution 1:500. Good luck Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Thu, Jan 24, 2019 at 1:23 PM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. BRCA1 on Benchmark Ultra (Curt) > > > -- > > Message: 1 > Date: Wed, 23 Jan 2019 20:54:32 + > From: Curt > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] BRCA1 on Benchmark Ultra > Message-ID: > > <9C8F910F72893643B3C3793C3D67132B94D0B4A3@PATHOLOGYSERVER.pathologyarts.local > > > > Content-Type: text/plain; charset="us-ascii" > > Anyone getting good results, I'm trying to determine which clone/vendor to > use... better to ask before I waste a bunch of time and money... any > suggestions or recommendations? > > > Curt > > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > > > -- > > Subject: Digest Footer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > > End of Histonet Digest, Vol 182, Issue 22 > * > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue blocks available for banking
Hello Curt, Will you give them to a private histology company like ours? We are constantly in need of human tissues for IHC/IF controls. If you will, we can give you our fedex or UPS account number. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.jmdhistology.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Sun, May 20, 2018 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. tissue blocks available for banking (Curt) >2. histo-off topic (RAY KOELLING) >3. Histopathology text recommendations (Gordon Brown) >4. Re: Histopathology text recommendations (Saundra Ellis) >5. Re: Histopathology text recommendations (Cristi Rigazio) > > > -- > > Message: 1 > Date: Sat, 19 May 2018 17:59:03 + > From: Curt < > > c.ta...@pathologyarts.com> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] tissue blocks available for banking > Message-ID: > <9C8F910F72893643B3C3793C3D67132B68BDA9BA@PATHOLOGYSERVER. > pathologyarts.local> > > Content-Type: text/plain; charset="us-ascii" > > If anyone is looking for tissue for a bank, I have a boxes and boxes of > derm specimens and GI specimens. I know they're probably not what everyone > needs but if someone out there does need them, you can have them. figured > I'd ask before I disposed of them... at the landfill next to the city water > well... LOL, just kidding, I know someone out there will not think that's > funny, it's just a joke. > > > Curt > > > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > > > -- > > Message: 2 > Date: Sat, 19 May 2018 12:06:38 -0700 (PDT) > From: RAY KOELLING <koelli...@comcast.net> > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histo-off topic > Message-ID: <578292945.1410691.1526756798...@connect.xfinity.com> > Content-Type: text/plain; charset=UTF-8 > > Hello all, > > > > returned late last night from Pittsburg and the Intel-International > Science and Engineering Fair, May 13-18. The largest most prestigious > pre-college STEM fair in the world. Took four high school students from > the Spokane region. One of our other "Team Washington" students from near > Seattle won the second place prize for the entire 1,400 project fair from > 81 countries and regions. A $50,000 scholarship with a chemical > engineering project. > > > While there, I viewed or walked by every project. Saw 50-60 with > histology images/histology projects. IF, IHC, H, Safrinin O, FISH, PAS, > decals, frozen, TMA's; anything and everything we all know. On > osteoarthritis, breast cancer, kidney disease, diabetic pancreas, neuro, > lymphoma's and more. > > > If you are able to help what could be a potential world class student in > the field of histology/pathology/medical and clinical research, hope you > will. > > > Sorry I was never able to get to the local histology school while my > students were being judged in Convention Center. Couldn't seem to ever > contact them.
[Histonet] Candida albicans
Does anyone have a block or two of Candida albicans to trade? We have a very diverse block collection to trade with. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PTEN Question (Evan Solanki)
Hello, I am by no expert in PTEN expression but we do a fair amount of antibody workups. For new antibodies that we don't have known protocols and or types of tissues we refer to "the human protein atlas". This gives volumes of information like both normal and abnormal tissue expression, and many other facts that may be relevant. Here is the link specifically to PTEN https://www.proteinatlas.org/ENSG0171862-PTEN/tissue Good luck! Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Tue, Oct 3, 2017 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. The NSH/SC was GREAT!! 2017 was GREAT!! And My phone is still > ringing with great opportunities in FL, NC, VA, CA, MA and > more!!! (Pam Barker) >2. PTEN Question (Evan Solanki) > > > -- > > Message: 1 > Date: Tue, 3 Oct 2017 11:27:03 -0400 > From: "Pam Barker" <rel...@earthlink.net> > To: "Histonetters Histonet" <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] The NSH/SC was GREAT!! 2017 was GREAT!! And My > phone isstill ringing with great opportunities in FL, NC, > VA, CA, MA > and more!!! > Message-ID: <006501d33c5c$117d52c0$3477f840$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters! > How are you doing? I had such a great time at the NSHSC right here in my > hometown ? Orlando, FL!!! > I enjoyed making new friends, reconnecting with old friends and learning > new > things. > It was touch and go for awhile there with Hurricane Irma?s unwelcome visit > but we are #orlandostrong and #histostrong > > We gave blood to support the victims of Hurricane Irma and the bloodmobile > ran out of bags!! > There was an amazing and inspiring speaker provided by Roche! > The hotel was absolutely gorgeous! > AND So much to see and do here in Orlando > > But the most fun of all? > I worked the registration desk with some really fun histotechs! > I posted some pictures on Facebook, Twitter and Instagram with the > Hashtag #NSHSC. > If you get a chance check them out!! > I really encourage everyone to go to meetings when they can. > If you can?t make the national meeting try to make it to a regional or > state > meeting. The camaraderie and educational opportunities are phenomenal. > > Next year the convention will be held in St. Louis, MO Hope to see you > there!!! > > ? My phone never stopped ringing while I was gone!! > ? All of my positions are full time permanent positions. > ? My clients offer excellent compensation, benefits and relocation > assistance. > **And they are ready to interview and hire right away!** > > I Have Management Opportunities in: > North Carolina > California > Florida > Alabama > Virginia > > I have HT/HTL and eligible positions in: > Florida > Alabama > North Carolina > Massachusetts > Tennessee > > Of course I can?t put all the information about these opportunities in an > e-mail. So if you or anyone you know might be interested in hearing more > about any of these positions or want help with a job search in another area > please contact me. I can be reached at 866-607-3542 or on my cell at > 407-353-5070 or rel...@earthlink.net. > > Remember it never hurts to look. > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > ?Pam M. Barker > ? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions >
Re: [Histonet] Histonet Digest, Vol 164, Issue 7
Hello "Humidity issues-desperate for help", We have this problem every year during the summer months. Our fix is to bottle all chemicals when not in use. We have a plethora of 1 liter nalgene glass bottles we use for chemical solutions. It works for us. Thank you Hans B Snyder Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Tue, Jul 11, 2017 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. Humidity issues-desperate for help (Trini Nguyen) >2. gloves (Lauren Sweeney) >3. It's A Sizzling Hot Summer And It's Not Just the Temps!! > RELIA Histology Careers Bulletin 7-11-2017 (Pam Barker) > > > -- > > Message: 1 > Date: Mon, 10 Jul 2017 13:01:14 -0500 > From: Trini Nguyen <nguy0...@gmail.com> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Humidity issues-desperate for help > Message-ID: > <CACtXwcun+o_GqxSgZViX+sfgHG_ow9J4N0HjNTejGD6WH4uH_w@mail. > gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello Histonetters! > > I am currently working in a Mohs lab in Minnesota and during the winter > time we have no issues with humidity but comes summer, my slides get washed > out by the end of the day. I have tried everything I can think of but > NOTHING works. > > I have tried changing solutions throughout the day, keeping the stainer lid > open, adding molecular sieve in my alcohols and xylene substitutes, and a > dehumidifier. > > We have the mini Thermo linear stainer under an airfiltronix filter which > adds to the humidity issue. > > If anybody out there has other solutions I haven't tried yet please send > them my way! > > One more thing that I have heard a long time ago at a conference is that if > you use xylene substitutes then you do not have to use a hood/any type of > ventilation system. Does that sound correct to anyone? Is that statement > true? > > I have worked in labs that coverslip straight from alcohol and a > ventilation system is not required but I notice the staining is not optimal > in the long run. > > > -- > > Message: 2 > Date: Mon, 10 Jul 2017 19:31:52 + > From: Lauren Sweeney <lmari...@uga.edu> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] gloves > Message-ID: > <BN6PR02MB31376071DCDA4CDD20D0B56DC5A90@BN6PR02MB3137. > namprd02.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hello HistoWorld, > > My lab is desperately trying to find where to purchase "Cranberry Aqua > Source Nitrile Gloves" and having no luck. Going to the website listed on > the box of gloves, there is no way to purchase the gloves online. It just > says 'to order contact your local dealer'. I called their 800 number and > they said that you have to contact a distributer and they recommended Henry > Schien. When I contacted Henry Schien they were very snobbish on the phone > and said that there was no way to get a quote from them if we aren't > already "members" and account holders with Henry Schien. Just thought I > would ask fellow histotechs if they use these gloves and if so, who their > distributer is. > > Thanks! :) > > > > > > > -- > > Message: 3 > Date: Tue, 11 Jul 2017 10:56:17 -0400 > From: "Pam Barker" <rel...@earthlink.net> > To: "Hist
[Histonet] DakoCytomation Plus pdf
Hello, Does anyone have the PDF manual for the DakoCytomation Plus they are willing to share? Looked through the archives, but didn't see anything and can't find online. Thank you Hans B Snyder Histologistics 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 h...@histologistics.com <ha...@histologistics.com> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hypercenter XP pdf manual
Hello All, I have been contacted by a colleague in need of a shandon hypercenter XP pdf manual. Does anyone have one they could email to me? I would be very appreciative. Best wishes Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com <ha...@histologistics.com> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] contents of Histonet Digest, Vol 145, Issue 10
Message: 3 Date: Wed, 9 Dec 2015 19:49:37 + From: "Vickroy, Timothy" <tvick...@hmc.psu.edu> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Use of microarrays or tissue rolls for immuno controls Message-ID: <32332277dfb64786a26649d55d2ec9f5@EXCHSRVR15.mshmc.local> Content-Type: text/plain; charset="us-ascii" We are looking at the possible use of microarrays or tissue rolls for immuno controls on a routine basis. I would like to talk to people who are currently doing their immunos this way. We run about 120 slides a day with about 170 different primaries. Anyone with previous experience in this area (good or bad) who has any suggestions, I would be glad to hear about them. Thank you, Tim Vickroy Hershey Medical Center Penn State University Hello Tim, We do not use TMA's but are affiliated with a company ( http://jmdhistology.com/) that makes them. They have fast turnaround times of 2 weeks at $5.00 per core. They use a new proprietary system that allows us to make them very fast and can have multiple different sizes in the same block. Just in case you need them. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com <ha...@histologistics.com> The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Thu, Dec 10, 2015 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >1. Cork-covered beakers (Morken, Timothy) >2. embalmed cadaver tissue (Nancy Schmitt) >3. Use of microarrays or tissue rolls for immuno controls > (Vickroy, Timothy) >4. Blog post--not work related (Lester Raff MD) >5. A bad link--again (Lester Raff MD) > > > -- > > Message: 1 > Date: Wed, 9 Dec 2015 18:29:26 + > From: "Morken, Timothy" <timothy.mor...@ucsf.edu> > To: Histonet <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Cork-covered beakers > Message-ID: > <761e2b5697f795489c8710bcc72141ff60335...@ex07.net.ucsf.edu> > Content-Type: text/plain; charset="us-ascii" > > Hi all, we have some old beakers that we use for hot solutions that have > cork covering over the vertical portion of the beaker The cork insulates > for easy handling. I have not been able to find replacements for these. > Does anyone have a source for these beakers? Or a method to cover them with > the cork? > > Thanks! > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -- > > Message: 2 > Date: Wed, 9 Dec 2015 18:34:07 + > From: Nancy Schmitt <nancy_schm...@pa-ucl.com> > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] embalmed cadaver tissue > Message-ID: <6d1a978a1371471b80d67453f278d...@mercury.wad.pa-ucl.com> > Content-Type: text/plain; charset="us-ascii" > > Good Afternoon! > Does anyone work with embalmed cadaver tissue? How is this handled for > successful processing? > Any advice appreciated. > Nancy > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > informa
Re: [Histonet] Cracking paraffin blocks
Hello Tim, I also have had this cracking problem in the past but since resolved. I'm not sure about the specifics of the cracking problem but the cold plate might have something to do with it. Our cold plate is set to -5C and use Paraplast from McCormick Scientific. Our paraffin is roughly the same melting temp as yours, so probably not much difference. Unfortunately trial and error is apart of our jobs since there is relatively little uniformity in histology equipment and products. Have you tried setting the cold plate to a warmer temperature yet? It's worth a try and if that doesn't work maybe the paraffin has been getting too hot. I know when the paraffin gets too hot, the wax and plastic separate or break down and cause inconsistencies in the paraffin. To test this, take an external thermometer and place inside the paraffin tank. Then record the temp every hour for some time. This will tell you if the tank itself is a consistent temperature. Also, are the blocks taken off the cold plate and immediately jarred loose from the mold? Sometimes when I do this the shock of pried out of the mold can cause the paraffin to become brittle and break rather than bend. Let me know how it goes. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. twheel...@mclean.harvard.edu wrote: Hi Everyone: Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. I am having problems now with blocks developing cracks on the cold plate. The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. I use Surgipath Embedding Media (EM-400). The surface of the Valida's cold plate is -14C. The company has not seen this happening before, but they are looking into this further. Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. I do not think that this is a problem inherent with any particular machine. Has anyone encountered this problem before? If so, how did you resolve it? Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly rougher texture. Could this produce a different way of conducting heat out of the paraffin block than the Shandon? Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? I have used the Shandon Embedding Center for over 24 years. I have never had a problem with blocks that crack. Thanks for any thoughts that you may have on this. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vibratome Worcester MA
Hello All, We have a client that needs vibratome services for mouse brains. We would like this to be in the area of Worcester MA but would settle for something within 50 miles. Can anyone recommend? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Heme boogers
That's the aluminum ammonium precipitating out, it happens with Harris also. Do you filter it before use? Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com On Jan 15, 2015, at 16:46, Ingles Claire cing...@uwhealth.org wrote: Hello All! We have been having an interesting problem with our Hematoxylin. It seems there are globules that form on the bottom of our hematoxylin wells in our automatic stainer (Leica XL). These globs are acellular, so it is not a fungus or something. We use a progressive Mayer's Hematoxylin formula as follows: 1000ml DI 50g Aluminum Ammonium Sulfate 1g Hematoxylin powder 1g Citric Acid 0.2g Sodium Iodate Anyone know what this might be? It doesn't affect the staining, but likes to hang on the bottom of the staining racks and is a bit gross. Plus it gets dragged into some of the following wells on occasion. Thanks! Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vibratome services Worcester MA
Hello All, We have a client that needs vibratome services for mouse brains. We would like this to be in the area of Worcester MA but would settle for something within 50 miles. Can anyone recommend? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] And other crazy stuff.
You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, I work in a research facility where I do everything. One day a botanist emailed me to cut pine on the cryostat and asked what the largest size should be. I told him 2 mm x 2mm and explained the process as he had no idea what I was going to do. I thought ok, what is pine? He brought 5 pieces of wood pieces (pine) and asked if they could be cut cross section at 10um with 5 sections per slide. Of course this is not something I have ever had expience in doing, and they are hard as a rock. The trick that ended up working, was to soak the wood in warm water for 30 minutes, embed in OCT and cut. After cutting the sections were all balled up, so I floated them on water and picked them up on the slide. The sections were still folded and looked horiffic in my mind but he loved them. He said was going to look at them under a Raman confocal and wanted to see the plant cell wall. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell powell...@mercer.edu wrote: Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Celestin Blue B
Hello, Just in case you didn't find this yet, Rowley Biochemical in Danvers MA sells this. http://www.rowleybio.com/order.php Good luck! Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Tue, Jan 6, 2015 at 5:18 PM, John Smallwood jrsmallw...@bell.net wrote: Hello Histologists: Is there anywhere to get this ? Celestin Blue B C.I. 51050 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat troublshooting. CM3050
Hello Patrick, We have this same cryostat except it's the CM 3050S. Ours also has this issue usually after it's been running for many months with a defrost. The typical cause of your problem is the cylinder on which the block holder is attached has ice crystals or is frozen. I feel the cause of this is because the glass was left open too long while cutting. This allowed moister from the room to condense on the cylinder and subsequently causes a stop go motion. Since I am in charge of all histology equipment maintenance, my solution is to turn off the cryostat, take the microtome out of the cavity and let it dry overnight. Then in the morning, I physically dry the microtome. Using cryostat oil, oil all the moving parts including the cylinder, then wipe off excess. Before putting the microtome back into the cavity, the cavity itself must also be thoroughly dried. The final cool down process usually takes about 1-2 hours. Feel free to email any specific questions. Hope this helps. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Wed, Jan 7, 2015 at 6:42 PM, Lewis, Patrick patrick.le...@seattlechildrens.org wrote: H everyone, We have been using this old CM3050 cryostat for our Frozen OCT blocks. I am cutting at 5 uM. My issue is that when I use the hand rotator to cut my sections it doesn't seem to advance with each rotation. It can take as many as 4 rotations to go from one section to another. What would cause this, and is there an easy solution to this problem? I can use the button to automatically move the chick holder all the forward and back from blinking to blinking, so I am wondering if ice crystals or debris can be ruled out as the issue. Any advice welcome. Thanks Patrick. ps: when I do get the blade to cut into the block, I have to wonder if the section I am getting is really 5 uM, or is it thicker. Sometimes you can tell if the section looks particularly thick, but other times it's hard to tell. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Formic acid disposal?
I believe it's spam or a scam. Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com On Jan 5, 2015, at 13:25, Roy, Ryan ryan@va.gov wrote: I got this to.. Can an administrator explain or delete the source? thanks -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, January 05, 2015 1:22 PM To: remove Subject: [EXTERNAL] FW: [Histonet] RE: Formic acid disposal? I keep getting these emails. Since I can't read in whatever language this is I don't know what it says. They are asking for verification. What does this mean? In this day I don't just go putting my info out there to anyone. Is this person legit? Is anybody else getting these? Andi G. From: moine...@mokpo.ac.kr [moine...@mokpo.ac.kr] Sent: Monday, January 05, 2015 11:13 AM To: Grantham, Andrea L - (algranth) Subject: RE: [Histonet] RE: Formic acid disposal? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/logo.gif] ★스팸메일차단을 위한 인증 서비스 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] 편지 감사합니다. 저는 moine...@mokpo.ac.kr 이메일주소 사용자 입니다 국립목포대학교 웹메일은 스팸방지를 위해 인증서비스를 시행하고 있습니다. 따라서 저에게 편지를 보내시려면 처음 한 차례의 인증이 완료되어, 저의 주소록에 귀하가 등록이 되어야만, 저에게 정상적으로 메일을 전송할 수 있습니다. 본 인증 이후 저의 주소록에 등록되면,그 후 발송되는 메일은 인증과정이 생략됩니다 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button.gif]http://mmp.mokpo.ac.kr:8886/mmpmgr/user/auth_view.php?msgid=54AAD4507E25 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] 경고: 30일 이내에 인증번호를 전송하지 않을 경우, 보관된 메일이 삭제되오니, 본 메일 확인 후, 꼭 인증번호를 입력해 주시기 바랍니다. [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] ★Confirmation Service for Spam free [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] Thank you for your email This is moine...@mokpo.ac.kr Please complete the verification so that I can receive your email. “After finishing this verification, you can be in my address book forever and won't need to do it again.” [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button_1.gif]http://mmp.mokpo.ac.kr:8886/mmpmgr/user/auth_view.php?msgid=54AAD4507E25 [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] Warning: you must c_img to deliver the mail, or it will be droped in 30 days! [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/bottom.gif] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for 2 Gram pos/neg blocks
Hello All, I'm almost out of my gram pos/neg block. Would anyone be willing to trade for other types of tissue? I have AFB, iron, copper, amyloid. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] THICK AND THIN SECTIONS ?
We have a Leica 2125 that I have been servicing for 2+ years and have had no problems at all. I think anyone with an engineering background could do it. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Tue, Dec 16, 2014 at 10:51 AM, Roy, Ryan ryan@va.gov wrote: Probably, Also, just because a manufacturer doesn't sell a part doesn't mean you can't get a part somewhere else. Ryan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, December 16, 2014 10:00 AM To: Klaus Dern; histonet Subject: [EXTERNAL] RE: [Histonet] THICK AND THIN SECTIONS ? This is at least the third time I have seen this message. Is this a form of advertisement on the Histonet? Grumpy in San Diego because it is supposed to rain today. From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] on behalf of Klaus Dern [ klaus.der...@gmail.com] Sent: Tuesday, December 16, 2014 6:01 AM To: histonet Subject: [Histonet] THICK AND THIN SECTIONS ? If you are using one of the following Microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG2035 LEICA - CM 1850 Cryostat SAKURA SRM200 You could be faced with purchasing a new Microtome because these models are no longer supported by the manufacturer. ( No parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.der...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] EDTA decalcification solution
Hello, We make our own formic acid EDTA for a slow decal solution. Is this what you are looking for? Thanks Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com On Dec 1, 2014, at 14:20, Martha Ward-Pathology mw...@wakehealth.edu wrote: I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this?Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use itlength of time in solution, etc. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Staining for plant peroxidase
Hello All, We are in need of help finding sources for plant peroxidase staining. If someone could point me in the right direction, a link, papers or books, I am happy to research. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP
Hello All, Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14] (ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination. We are doing this by hand. The recommended dilution for this antibody is 1:50 with HIER. See used protocol below. So far I have tried: 1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining. 2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground 3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes. 4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C. 5. Tried blocking using 3 different serums, first each one then combinations and longer times. 6. Tried cutting the DAB concentration in 1/2 then 1/3. We have not tried enzyme or acid epitope retrieval yet. *Does anyone have a working protocol or suggestions on what to try?* Thank you in advance. Procedure: Deparaffinize 1. Heat slides to 60C for 10 minutes (oven). 2. Dry slides at room temp 5 minutes. 3. Xylene 5 minutes. 4. Xylene 5 minutes. 5. 100% ethanol 5 minutes (dehydration). 6. 100% ethanol 2 minutes (dehydration). 7. 95% ethanol for 2 minutes (rehydration). 8. 70% ethanol for 2 minutes (rehydration). 9. Run in DI water for 10 minutes. Antigen Retrieval 1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes. 2. Cold running water 5 minutes. Staining 1. Wash in PBS for 1 min. 2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal bovine serum for 2 hours. 3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum in PBS. 4. Wash 3x in PBS/tween for 5 minutes each. 5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each. 6. Wash 3x PBS 2 minutes each. 7. Incubate with anti-Rat Ig impress solution (according to vector’s instructions) 45 minutes. 8. Wash 3x in PBS for 5 minutes each. 9. Prepare Impact DAB substrate (see insert). 10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds). 11. Stop DAB reaction using deionized water. 12. Wash in DH2O for 5 minutes 13. Counterstain in Harris hematoxylin for 30 seconds. 14. Wash in DH2O for 5 minutes. 15. Dehydrate 2x in 95% alcohol for 30 seconds each. 16. Dehydrate 3x in 100% alcohol for 2 minutes each. 17. Clear 2x in xylene 5 minutes each. 18. Mount coverslips using Leica mounting media. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] peroxidase staining for plants
Hello All, I am not familiar with staining plants for peroxidase, so I thought I would ask you. Does anyone out there do peroxidase staining on plants? I have done myeloperoxidase staining on animal tissue. Is this the same for plants? Most of the plant antibody sites I have gone to do not list peroxides as a test. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Neutrophil staining
Hello All, I am looking to stain neutrophils using special stains and antibodies in mice tissue. I wanted your opinion about the best methods to do so. I am thinking of using the may Grunwald giemsa and T-blue for specials and myeloperoxidase and Ly6G for the antibodies. What are your favorite special stains and antibodies to stain for neutrophils? Best regards Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need microvascular (MVD) staining and quantification of tumro vasculature.
Hello All, I have a customer who needs microvascular (MVD) staining and quantification of tumro vasculature, where staining is typically done using CD31. Is there anyone who can do this and willing to forward their information to me? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RI company seeks plastics company for research work
Hello All, I have a company who needs: a source of basic services for sectioning of GMA blocks containing biomaterial devices and subsequent HE staining. The devices are cut for embedding to about 8mm in length, and contain adhesives, thermoplastic membranes, various scaffolds, and cells. Our group would do the embedding and transfer samples to you, and would be looking for about 25-50 samples/month. I would like to give him references so that he can email and or call places he chooses. Please email me and I will forward all information. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Responses to Plastics
Hello All, Thank you to all who responded to my query about the RI company. I have forwarded all your emails directly to the company. Who ever has the pleasure to service the company, you have my gratitude. Best Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Schiff : room temp or not
Hello Amber, That depends on your protocol. Which protocol are you using and what does your protocol say to use the schiffs at? Consider that schiffs is usually stored at 4C in brown or opaque bottles. This suggests it is light sensitive. Our protocol say to use the schiffs at 37C for 15 minutes but I have used it at 22C for 30 minutes and at 60C for 10 minutes with consistent results. We have found that using it at 60C reduces it's shelf life considerably and do not suggest it unless you are familiar with doing so. Good luck Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie amber.mcken...@gastrodocs.net wrote: When staining PAS's, does the Schiff need to be room temp or does it matter? Just curious about when to add it to our Sakura stainer. Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Schiff : room temp or not
There is no doubt, manufactures prefer shorter life. This case is exempt when one is producing solutions for themselves. Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com On Jul 30, 2014, at 8:32 PM, Michael Backhus mikeykk...@live.com wrote: Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. Mike Sent from my iPhone On Jul 30, 2014, at 8:14 PM, Hans B Snyder h...@histologistics.com wrote: Hello Amber, That depends on your protocol. Which protocol are you using and what does your protocol say to use the schiffs at? Consider that schiffs is usually stored at 4C in brown or opaque bottles. This suggests it is light sensitive. Our protocol say to use the schiffs at 37C for 15 minutes but I have used it at 22C for 30 minutes and at 60C for 10 minutes with consistent results. We have found that using it at 60C reduces it's shelf life considerably and do not suggest it unless you are familiar with doing so. Good luck Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie amber.mcken...@gastrodocs.net wrote: When staining PAS's, does the Schiff need to be room temp or does it matter? Just curious about when to add it to our Sakura stainer. Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Freelance Histology
Several years ago while in the beginning stages of setting up my histology lab, I looked into this possibility (cutting at home). The town hall laughed and said it is illegal for a business to run out of ones house that uses chemicals and paraffin because of it's flammable and explosive properties. This made sense, but I also had heard stories of beginning companies who have done this. Therefore a business running out of a residential house is doing so illegally and maybe without insurance. Just my two cents. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Tue, Apr 22, 2014 at 6:07 PM, H R hrfulk...@gmail.com wrote: Has anyone heard of people cutting and staining slides out of your garage or some kind of similar set up, like picking up overflow from different companies /labs? I overheard a conversation about someone doing this in their retirement. Labs send them the block and all they do is cut and do HE stain and return both and bill? What kinds of regulations would that entail? I don't know if that could be true. Maybe for research facilities? -- *H* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need very good used microscope
We are looking for a used microscope with very good optics that can take a camera. Would like to spend no more that $2000.00. Does anyone have suggestions? Thank you Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] aqueous mounting medium
Hello, I have one for ORO but not for IF. If someone has one for IF, I would be interested also. Thank you Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com On Feb 19, 2014, at 12:27, Jennifer MacDonald jmacdon...@mtsac.edu wrote: Does anyone have a good recipe for aqueous mounting medium? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How to De-stain trichrome
Does anyone know how to destain Massons trichrome? Is it possible? Thank you Histologistics Hans B Snyder 508.308.7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Soaking artifact
Hello Deanna, This sounds like BS to me, maybe just a idiosyncrasy with their management. They are probably set in their ways and refuse to change or believe in another way to do it. It might be that one person once had artifact and it caused an uproar for the pathologists who in turn implemented this rule. I know, that if some kinds of tissue is soaked too long, the tissue can bubble up out of the block and be rendered unusable. If I were you, I would just go along with their way of cutting and don't try to change them. Change is only possible if someone is open to it. Best Regards Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Sun, Jan 5, 2014 at 4:42 PM, Deanna Leslie dea.les...@gmail.com wrote: Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Methyl Carnoy's processing
Hello, I have a researcher who has fixed their specimens in methyl Carnoy's but would like to skip the methyl benzoate step before processing (we do not have methyl benzoate). Does anyone have another method that works well without the methyl benzoate? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Aspergillus tissue blocks for controls
We have tried the moldy peel also but the pathologists did not like this and would not accept any controls of this form. I don't blame them they want to see it naturally occurring. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Mon, Nov 4, 2013 at 3:07 PM, Patrick Laurie foreig...@gmail.com wrote: Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim vickroy@mhsil.com wrote: We are in desperate needs of obtaining GMS controls. In the old days we had all the fungal specimens we needed. Today it is getting hard to find these tissues or controls. Does anyone have any idea where we can get tissue blocks with Aspergillus or does anyone have any other suggestions about GMS controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] StainMate Stainer
Hello, Does anyone know if the Stainmate stainer can be programed with 50 steps or more? I have looking to buy one but need it for a purpose other than cytology and histology. It needs to be have 50 steps or more for this application. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used cytology stainer
Hello, We are in need of a super small slide stainer, anything in working condition that is programable. Does anyone have an extra stainer they would like to get rid of? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AFB Controls
I know source medical products has them cheaper than newcomer supply. E-mail and ask for Walter. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Thu, Oct 10, 2013 at 5:24 PM, Cheryl tkngfl...@yahoo.com wrote: Help? We are about to run out of AFB control slides and haven't had a good loaded case in a while. Is there an easy way to come up with an AFB positive block or could someone lend me one to be replaced at a later date? (Go through too many to be cost effective to buy) OR is there something out in the world I can use to make a control? Please respond to tkngfl...@yahoo.com Cheryl Kerry, HT(ASCP) 281.852.9457 Office 800.756.3309 Phone Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Losing tissue on IHC slides
I agree water is always a potential issue. Tissue can be finicky though. Have you tried poly-L-lysine coated slides? They do help is some occasions. If you need a protocol, let me know, I can write one up, very easy. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com On Fri, Jul 26, 2013 at 5:38 PM, Tony Auge tony.a...@gmail.com wrote: How are you drying your slides? If in an oven, what's the temp and duration? Try going longer and tap off excess water before drying. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] luciferase use
Hello, Does anyone have a luciferase or RFP protocol for labeling cells in vitro? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How do you label myoblasts and fibroblasts in cell culture to show up after paraffin processing?
We are looking to differentiate between myoblasts and fibroblasts, Is there IHC or IF that can do this after paraffin processing? We were also wondering if it is possible to label these two prior to processing? Thank you all -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TBS tissue processor single mode vs group mode
Hello All, We just got a TBS APT1-120 tissue processor donated to us. I am would like to hear from anyone about the single mode verse the group mode of operation. What your opinoins about using one verse the other. I hope to set this up this week and would like advice on which is better. Thank you -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: [Histonet] Helicobater Pylori QC block and/or slides
-- Forwarded message -- From: Hans B Snyder h...@histologistics.com Date: Fri, Jan 4, 2013 at 5:32 PM Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides To: Tasha Fraser tfra...@olympicmedical.org We have a human colon tissue that is positive. On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser tfra...@olympicmedical.orgwrote: Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Helicobater Pylori QC block and/or slides
We are a private histology lab that is willing to share. If you need slides or blocks let us know! thanks Sent from my iPad On Jan 4, 2013, at 8:06 PM, Hans B Snyder h...@histologistics.com wrote: -- Forwarded message -- From: Hans B Snyder h...@histologistics.com Date: Fri, Jan 4, 2013 at 5:32 PM Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides To: Tasha Fraser tfra...@olympicmedical.org We have a human colon tissue that is positive. On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser tfra...@olympicmedical.org wrote: Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Does Formalin fixed frozen sections for antibodies need to be kept frozen?
Hello I am working at a research facility that has thousands of formalin fixed frozen heart sections in their freezers. They have been led to believe that this is necessary for their antibodies to work. I do not believe this is the case. Does anyone know of antibodies that will lose their antigenicity if stored at room temp? Thanks -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Sales Help Needed!
Histology Sales Help Needed! Histologistics Inc. Located in Worcester, MA is a histology service company. We are looking for a talented sales representative who is willing to work for huge comission. We provide routine histology services such as processing, embedding, cutting, HE and special stains. All of our techs are HT certified. First time/one time only clients and repeat clients have the same commision, even years after the original sale is made. Any new clients who refer to this histonet posting will recieve a permanant discount equal to the sales commision. Please contact us or post in histonet if you have questions or are interested in helping! Thank you histoneters. Benjamin S Ferland Histologistics 100 Barber Ave Worcester, MA 01606 617-710-4870 benja...@histologistics.com -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histomorphometrics done for bone samples
Hello, I have a Dr. who is asking about how to get histomorphometrics done for bone samples, data needed is % of new bone present in samples from sites grafted with freeze-dried mineralized cancellous allograft bone. We can do the plastics on it but the computer assisted part, I do not know anything about. Thanks Hans B Snyder Histologistics 100 Barber ave Worcester, MA 01606 www.histologistics.com h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
