[Histonet] Cutting paraffin sections on a cryostat operated at room temperature? Nope.
Ever tried turning the handle of the cryostat, when it's at room temperature? Cryostats are tooled manufactured to operate at a low temperature. Since metal contracts at the low temperature, you'll find that you can't operate the microtome at the higher temperature. The handle will barely move. Sandy Harrison, HTL (ASCP) Histology Supervisor Minneapolis VAHCS 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Countertop fume hoods
Dear Merissa, I have used the Labconco countertop fume hood in a few different Histology settings, both MOHS and general, and found it to be very compact, easy to change the filter, and very effective at pulling the fumes away from the user. It is also a ductless system. See http://www.labconco.com/product/fume-adsorbers/35 Sincerely, Sandy Harrison VA Histology Supervisor, Minneapolis, MN 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of M.O. Sent: Thursday, April 11, 2013 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Countertop fume hoods Hello Histonet! I am looking into small countertop fume hoods or maybe a filter system that I can use to place tissue under that has been in formalin or decalcifier to diminish the fumes. Do you have any recommendation on what and where to look for something like this? Thank you! - Merissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica 4020 linear stainer
Could I get some feedback on linear stainers?Has anyone had any experience with the Leica ST4020? We are going to purchase a linear stainer for a new MOHS laboratory. Thanks, Sandy Harrison Histology Supervisor VA Health Care Systems, Minneapolis 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] procedure for destaining a cytology slide followed by restaining with a special stain, such as PAS?
Does anyone have a procedure for restaining a cytology slide? We experienced a loss of cells on an FNA aspirate of a lymph node, which had been originally stained with Diff Qwik. The procedure we used was as follows: 1) Destain using 1% Acid Alcohol (1 ml. concentrated HCL added to 99 ml. 70% alcohol) for approx. 30-60 seconds. 2) Restain on automated stainer (Ventana) with PAS kit. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H.Pylori
We do an H.P. routinely on stomach, body, fundus, antrum, pyloric, prepyloric, and gastric (Not on cardia area of stomach or GE jcn.) We use a kit from Newcomer Supply of a Steiner-Chapman modified Silver Stain. The pathologist's order an IHC for H.P. only about 5% of the time, if for example, they do not see H.P. from the silver stain, but the patient has a history of H.P. Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Are those sites using the Thermo Fisher Slide Mate slide printer also using Thermo slides?
I just purchased 6 Slide Mates. We just began using them yesterday. Are any of you using slides that are NOT Fisher slides on your SlideMates? When you respond, could you answer the following questions? a) Is the print job consistently satisfactory? b) How long have had your SlideMate(s)? c) What difficulties did you have, getting it to consistently print well? d) Approximately how many slides per day you are printing on (each) SlideMate? Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BOND IHC instrument- anyone experiencing multiple heater failures??
We have 2 Bond Max instruments and have experienced multiple heaters getting contaminated and corroded underneath, with a particulate matter, causing the heaters to fail. Our 2nd BOND Max is 3 years old and we have had to replace a total of 12 heaters (out of 30.) The only thing we do that is non-standard is we have been dewaxing off line, to save time. Does anyone else dewax off line? Is anyone else experiencing heater failures? We did a major P.M. 3 months ago, including changing the aspirating probe. That not-withstanding, we had to replace 5 more heaters last week. Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
Leica Autostainer XL with CV5030 coverslipper and transfer station. This has been a real timesaver for us. It automatically moves the slides from the stainer to the coverslipper. It has been relatively trouble free. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Wednesday, July 18, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated H+E and cover slipper Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Mohs
Depends ;-) All kidding aside, there are factors that weigh in, such as geography, availability, training, experience, speed, etc. 10 years ago, when I worked as a Mohs' tech in the Denver, Colorado area, I received $25.00 per hour, plus benefits. I usually only worked 3 days per week, but on some rare occasions, worked till 9 p.m. trying to get to clear margins. I don't know what the current wages are. Sandy Harrison, HTL Histology Supervisor, Minneapolis VA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rebecca a. Johnson Sent: Monday, July 02, 2012 5:37 PM To: histonet Subject: [Histonet] Mohs Need to know what Mohs techs are getting paid. Thanks Becky ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity Check
Yes, we have a wireless temperature monitoring system, called Checkpoint, and each room has a sensor that monitors temp and humidity. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of lau...@blufrogpath.com Sent: Monday, July 02, 2012 3:20 PM To: Histonet post Subject: [Histonet] Humidity Check Do others check the humidity of their Histo lab on a daily basis?= Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paperless Histology
And what about a paperless procedure manual? We have an electronic system, called Proquis, for our manuals. It sends an e-mail to all the appropriate people, when there is a new procedure to review or when old procedures are due for review. Pretty cool. The only drawback is, most of the time, people would rather grab a notebook to look up a procedure, rather than the cumbersome process of logging in, clicking thru a bunch of menus to view what you could have physically touched and seen in seconds. Plus, you better have a lot of terminals. Or better yet, let's issue I-Pads to all employees. ;-) We live in a brave new world! Sandy H. Histology Supervisor, VA Minneapolis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Friday, June 29, 2012 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Histology My director would like for our histology lab to go totally paperless. Is there a lab out there that is already paperless? Are there any software programs for this? It seems to me that this would include; gross sheets, logging in specimens, AQ sheets that are delivered with slides, decal log, special stains request. Would appreciate all the info. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] compatibility of Thermo slide printers with Leica cassette printer
1) Does anyone have a Leica Cassette Printer on which they are printing 2-Dimensional barcodes? 2) Is anyone using a Thermo slide printer, with bar code reader, to read cassettes printed off of a Leica Cassette Printer with 2-D bar codes? Thanks for your input. Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chicago Sky Blue stain vs. KOH - striking comparison
Does anyone have ordering information for a stain called Chicago Sky Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE (page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain. The results were dramatic, with about a 25-50% increase in the Dermatophytes identified and about a 5X increase in the Pityriasis versicolor identification. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] saving slides between levels for IHC?
Does anyone know if it is standard to save sections from between the levels for IP? Is it standard at your institution? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica cassette and slide printer ink
I have been told that I can no longer buy only the replacement ink cartridges for Leica cassette and slide printers. The ink cartridge was already an outrageous $411.00 each. Now you have to by a kit for $566.00 each. The kit includes a replacement plate along with the ink. Why in the world the replacement plate would need to be replaced every time you replace an expired ink cartridge is beyond me, other than a shameless scam to up the price on the already completely overpriced ink cartridge. Has everybody been told this by their Leica distributor? If so, I think a lot of us will be looking HARD at other instruments when it comes time to replace slide/cassette printers! Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gout processing procedure
Could someone share their gout processing procedure? The specimen is fresh, not in formalin. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bluing
Hi Dorothy, Try Richard Allan Bluing Reagent. Here's what they say about their product: It is a buffered product that ensures the proper alkalinity (pH=8.0). Unlike ammonia and lithium carbonate, RA's Bluing Reagent does not allow for the pH shift which can affect the crispness of nuclear detail. When I first began supervising this lab 5 years ago, they made from scratch their own hematoxylin and eosin (not to mention buffered formalin.) Unfortunately, the quality of the stain was very spotty and caused the Pathologists a lot of problems. I switched us to the Richard Allan 7211 Hematoxylin, which has beautiful, crisp nuclear detail. We also went with the recommended Richard Allan Clarifier, Bluing and Eosin, as well, so that we could produce a consistently high quality HE every time. In 5 years, we've had very few complaints about the stain from the 8-10 Pathologists we've worked with, except for one occasion when there was some isolated nuclear hazing. We did some detective work and determined that the cause was due to a rack or two that had been placed in the oven without properly removing the excess water or draining of the slides before placing them in the oven. Have a great week-end everybody. It's practically the 1st sunny day we've had, here in Minneapolis, for the past 2 weeks and Saturday and Sunday's forecast looks good, too! Sandy Harrison VA-Minneapolis Supervisor, Anatomical and Surgical Pathology 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, June 24, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my bluing step in the HE process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question on H pylori
We do either IHC or a Modified Steiner, depending on the Pathologist's preference. We switched to Newcomer Supply's Steiner-Chapman Modified Silver Stain Kit about a year ago, because it eliminated the use of Uranyl Nitrate. We have been delighted with the consistency of the stain. Sandy Harrison, HTL (ASCP) Anatomical and Surgical Pathology Supervisor, VAMC Minneapolis 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-requ...@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori..is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Input needed
Out of 6 recent hires, I hired two 50+ employees. One had been employed in another department for over 28 years. One was a fairly recent grad. of a Histology program. They were both good employees, though neither had much routine Histology experience. That being said, neither one of them are still here: one chose to retire, after 3 years in our department. The other one decided to return to the previous place of employment within 6 months, stating that the fast pace of our lab was a deciding factor. Bottom line - neither age or gender played a role in their getting hired. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, April 22, 2011 8:11 AM To: Mary; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Input needed Age and gender should not play a role in your chances of getting a job. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mary Sent: Thursday, April 21, 2011 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Input needed What's the chance of getting a job in histology as a new grad at age 57. Will gender be an issue? Thanks for the input. Jason ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
We dry the control slide at the same time that we dry the tissue being stained, since controls are supposed to be handled in the same manner as the test tissue. That being said, I guess if we were truly following that rule, we would cut the control on the same day we cut the test sample. :-) Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, March 10, 2011 6:39 PM To: Amador, Amanda; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control Slides I've noticed that our spirochete control slides don't stain as intense starting about 3 months after they are sectioned, and that they stop staining by about 6 months. (This is with a silver stain.) I've talked with other histotechs, and they say they've seen the same phenomenon with AFB and Gram controls. (We use ours up too quickly - we never have 6-12 months old control slides for these, so I can't attest to this. ) Bancroft's book talks about this phenomenon with amyloid, and suggests that oxidation of the tissue (proteins) due to exposure to air may be the cause. I'm guessing the it probably applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after drying, and then place the box in the refrig, as cold slows down the chemical change, and the lid keeps the moving air off the slide. Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. Just 3 suggestions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Amador, Amanda aama...@ameripath.com Sent: Wednesday, March 09, 2011 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] No Mail??
Wow...didn't realize how addicted to Histonet I'd become till this week! Sandy Harrison Histology Supervisor VA Medical Center Minneapolis, MN 55417 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 9:54 AM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No Mail?? And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
CBG Biotech. Their contact info is 1-800-941-9484 or you can e mail them at i...@cbgbiotech.com. I purchased my recycler through them and have ordered extra hydrometers from them, too. They give excellent customer service. Sandy Harrison, HTL(ASCP) Histology Supervisor, VA Minneapolis 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dkb...@chs.net Sent: Tuesday, November 23, 2010 2:48 PM To: Vickroy, Jim Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Vickroy, Jim vickroy@mhsil.com Sent by: histonet-boun...@lists.utsouthwestern.edu 11/23/2010 02:07 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] CHECKING ALCOHOL CONCENTRATIONS Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner)
I'm looking at the Slide Mate and Print Mate, too. Currently, I have the Leica cassette and slide printers. They have really been great. Reliable and relatively problem free. But they are so large. I'd like to find a slide printer that has a small footprint, like the Slide Mate, so that we can place one at each microtomy station. Assuming there are other slide printers out there, besides ThermoFisher's Slide Mate and Leica's, what are their pro's and con's? Sandy Harrison -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, November 15, 2010 9:29 AM To: Damaris Beil; histonet Subject: RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block release
Here's what the recently revised CAP checklist says about releasing blocks; scroll down to Note 2: **REVISED** 06/17/2010 ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than state and federal regulations, are: 1. Accession log records - 2 years 2. Wet tissue (stock bottle) - 2 weeks after final report 3. Paraffin blocks - 10 years (subject to Note 2, below) 4. Glass slides (including control slides) and reports - 10 years (slides must remain readable for this period) 5. Surgical pathology reports - 10 years (see Notes 3 and 4, below) 6. Fluorochrome-stained slides - at the discretion of the laboratory director 7. Fine needle aspiration slides - 10 years 8. Images of FISH studies - 10 years (see Note 5, below) There must be a documented policy for protecting and preserving the integrity and retrieval of surgical pathology materials and records.The retention period should be extended, when appropriate, to provide documentation for adequate quality control and medical care. NOTE 2: Regarding release of blocks for research purposes: Federal regulations require that a laboratory retain paraffin blocks for two years. The CLA requires, however, that they must be kept for at least 10 years. Nevertheless, blocks may be released for research purposes after the two-year regulatory requirement if all of the following criteria are met: 1. The written consent of the patient is obtained. For laboratories subject to U.S. regulations, the consent must include formal authorization in accordance with the requirements of HIPAA, if identifiable patient information is released. 2. The laboratory retains sufficient blocks to support the diagnosis for the full 10-year period. 3. Provision is made for retrieval by the laboratory of any blocks or material that remain after use in research, if the blocks or material are needed for diagnostic, legal, or other legitimate purposes. 4. The laboratory meets other relevant requirements including but not limited to the requirements of the institution, the directives of any applicable institutional review board (IRB) or similar entity; and state and local laws and regulations. NOTE 3: Pathology reports may be retained in either paper or electronic format. If retained in electronic format alone, however, the electronic reports must include a secure pathologist electronic signature. Images of paper reports--such as microfiche or PDF files--are acceptable. NOTE 4: Reports of outside consultations performed on cases from the laboratory (whether or not such consultation was requested by the laboratory) must be retained for 10 years after the date on which the original report was issued. NOTE 5: There is no retention requirement for images when the source slides remain readable for the required 10-year retention period. The 10-year retention requirement applies to images of slide preparations that are not readable for the 10-year period (e.g. FISH studies). 22 of 44 VA Med Ctr - Minneapolis Path Lab Med Service 113 Anatomic Pathology Checklist 06.17.2010 Evidence of Compliance: ✓ Written record and specimen retention policy(ies) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, October 18, 2010 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block release Everyone, I have been asked to post a query as to what your institution does in terms of releasing blocks for oncology clinical trials. Please respond as it is important to us as we receive a lot of those blocks here at Northwestern (policies, procedures, alternative submissions etc) If you are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette Labeler
We have the Leica Cassette Labeler. It works just fine. I have looked at the Thermo Fisher Cassette Printer, which had a much smaller footprint than the Leica. I would be tempted to go with the Thermo if I have to replace my current labeler. These are expensive instruments but worth having. It would be appropriate for your workload. Plus, these cassette labelers can print a bar code, in addition to the pt. id and name. Laboratories can eliminate numbering errors by utilizing bar code scanners throughout the entire work flow; at grossing, processing and microtomy. Bar coding can also assist you with implementing LEAN processes. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com Sent: Thursday, September 16, 2010 7:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler I work in a small lab and process approx 150 cassettes a day. We currently use a chemical resistant pen that works great. We write the number on top and the patient initials on the side. We are considering getting a cassette labeler. Is it really worth the expense for 150 blocks a day? Is it possible to enter information on the side of the cassette? Can you share some of your experience with different ones with me. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] accu-edge low profile microtome blades
About 1/2 of my tech.'s wipe the oil off; otherwise it causes the first 2-3 ribbons to curl off the knife, rather than forming a nice ribbon. Sandy Harrison Histology Supervisor Minneapolis VA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, September 01, 2010 1:27 PM To: Laurie Colbert; Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We use the low-profile microtome blades and love them. We actually found Thermo-Fisher's brand of the low-profile to work just as well and they are less expensive. We had problems with other manufacturers' low -profile blades that had the oil on them. However we have several sample boxes and I will have the techs wipe the oil off and see if they work better. Thanks, Laurie. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Mon 8/30/2010 4:25 PM To: Brandi Higgins; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] accu-edge low profile microtome blades We also use those blades and love them. We've tried others but always come back to the Accu-edge. I have always noticed a film of oil on the blades. I usually wipe the edge of the blade with a kimwipe before using it. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Monday, August 30, 2010 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] accu-edge low profile microtome blades Hello all, We use the accu-edge low profile microtome blades (exclusively, as they work best for us). We noticed an oil/lubricant of some sort on the blades in the last box that we opened (we checked one other box and it has the same, although none previously did, or at least we didn't notice it). The oil is giving us problems with our sectioning. Has anyone else noticed this, either now or in the past? Also, does anyone have a suggestions of other blades we should use? Thanks for your input, Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] QC on stained slides
You have included all the criteria; fixation, processing, embedding, microtomy, staining, coverslipping and labeling. If you were able to submit slides to CAP under their HQIP program, you would get graded evaluations. Short of that, could you perhaps send out duplicate slides to another local lab, or sister hospital, for peer review? You could offer to exchange slides on a twice yearly basis, since they, too, may be looking for additional quality control. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, August 02, 2010 2:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] QC on stained slides Hi all As part of a self assessment programme conducted by my employer, and related to my performance review and salary adjustment, I need to determine the criteria of what makes a stained slide acceptable or unacceptable. I was wondering if anyone out there had a checklist that they would be willing to share, that i could perhaps adapt. I realise that the easiest would be to send slides out for external control, but in this case it is not feasible. What I put together is this: - Quality of decalcification, processing, infiltration - Quality of sections (no wrinkles, missing bits, scores etc) - Entire representation of tissue area - staining pattern as expected according to protocol - coverslipped without bubbles or other inclusions - labelled neatly and correctly but, the question inmy mind is what would be the criteria that would make a slide merely adequate or truely outstanding? PLease help thank you -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] labeling of recycled chemicals
Dear Histonetters, If you use a recycler for xylene and/or alcohol, how are you labeling the recycled containers? Do you assign a lot # to each carboy? Do you keep a log or just label any container filled from a carboy with the lot # and the %, in the case of alcohols? Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Chemical outdates
If there are no expiration dates on the chemical: For chemicals distributed by Fisher, look at the Lot #. The first 2 numbers are the year it was manufactured. In general, chemicals are considered expired 3-5 years after the year they were manufactured. You can also check the certificate that comes when the chemical is delivered. It should have an expiration date or at least a year of manufacture. Sandy Harrison -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Thursday, January 21, 2010 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chemical outdates Hello Histonetters, We just got inspected by CAP and it was recommended that we put expiration dates on our chemicals once we open them. What kind of time frame do any of you other labs do in regards to this? Thanks for your input, Amylin Johnson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech opening at Minneapolis VA
DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN. Full time Histotech. opportunity at Minneapolis VA. BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp. IHC experience a plus. Effective interpersonal skills required. Holiday, evenings and weekends off. Excellent bene's. Detail oriented. Responsible for technical and procedural operations of the dept., performing quality control, quality improvement and regulatory compliance tasks. Job will be posted on www.usajobs.gov within the next month. Please contact me if you have any questions: Sandra Harrison, Histology Supervisor, at sandra.harris...@va.gov. Principals only. No recruiters please. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opening, Minneapolis VA
DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN. Full time Histotech. opportunity at Minneapolis VA. BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp. IHC experience a plus. Effective interpersonal skills required. Holiday, evenings and weekends off. Excellent bene's. Detail oriented. Responsible for technical and procedural operations of the dept., performing quality control, quality improvement and regulatory compliance tasks. Please e-mail resume to Sandra Harrison, Histology Supervisor, at sandra.harris...@va.gov. Principals only. No recruiters please. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] nuclear bubbling
How about microwaving to dry slides? Can that cause nuclear bubbling? When you say completely drained off, does that mean your slides have to be completely dry prior to placing them in the oven? Thanks, Sandy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 15, 2009 2:59 PM To: Histonet; Joyce Cline Subject: Re: [Histonet] nuclear bubbling Anybody can experience nuclear bubbling in any type tissue as long as the sections as set to dry at high temperature BEFORE they are completely drained off! René J. --- On Wed, 7/15/09, Joyce Cline jcl...@wchsys.org wrote: From: Joyce Cline jcl...@wchsys.org Subject: [Histonet] nuclear bubbling To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 15, 2009, 3:45 PM Has anyone experienced nuclear bubbling on prostate biopsies? Joyce * CONFIDENTIALITY NOTICE * This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin in Alcohol
polycyclic aromatic flourescent compounds that in high concentrations I wouldn't think the 3 mls of eosin dropped in the last 95% alcohol could be considered high concentration but that's what keeps Histonet entertaining; I learn something new every day. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Tuesday, July 14, 2009 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin in Alcohol A couple of weeks ago I posted the message below on the histonet and all of you responded that it shouldn't matter so I have finally gotten a reply from the company we send our prostate biopsies off to and below is their response. So now you know the rest of the story! We have used Eosin in the last 95% alcohol on the tissue processor for several years. I usually add approximately 5 ml to the full jug. It is a great tool to use for embedding. However, we received a letter from the lab that we send our prostate biopsies to saying that it was undesirable because it interfered with their immuno staining. They sent us some cobalt blue to use in the place of eosin along with mixing instructions and the whole batch of tissues came out such a dark blue. There is no delineations in the color of the blue and I found it to be useless for helping to embed. I would rather do without anything than use cobalt blue. I guess the point of my rambling is, Eosin is a wonderful tool to use unless you are doing immunos on prostate biopsies. Thanks, Jennifer Johnson, HTL (ASCP) Their reply: The problem is that eosin belongs to a family of polycyclic aromatic flourescent compounds that in high concentrations binds to and saturates all tissue components. When immunoflourescence is performed on such tissue- as in the prostate px+ test- the diffuse background autoflourescence signal from prior treatment with these compounds can interfere with, and even totally overwhelm, the signal of the flourescent-labeled antibodies used to localize biomarkers in the tissue. _ Lauren found her dream laptop. Find the PC that's right for you. http://www.microsoft.com/windows/choosepc/?ocid=ftp_val_wl_290__ _ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
