Re: [Histonet] [EXT] Histonet Digest, Vol 215, Issue 16
Hello Administrator, I would like to ask if it is okay or allow to post jobs on the histonet site? I have a friend who is in desperate need of help. Thanks, Thomas Thomas Huynh, BS, HT(ASCP) CM Chief Histology Laboratory Department of Veterinary Medicine & Surgery UT MD Anderson Cancer Center 1515 Holcombe Unit 063 thu...@mdanderson.org T 713-745-5077 Lab 713-792-2793 C 832-794-0741 True Colors: Gold -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: Friday, October 22, 2021 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [EXT] Histonet Digest, Vol 215, Issue 16 WARNING: This email originated from outside of MD Anderson. Please validate the sender's email address before clicking on links or attachments as they may not be safe. Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!034AOEtqBSB9JjzBTQzrgMy7HGZE4e3JivpZKpKN5l1xXYyLCZZJ2TnM3TF0D4jA$ or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Zebra Fish Histology Protocol
Good morning Histonetters, Happy Friday! Does anyone has a zebra fish histology protocol who willing to share with me? From fixation, decalcifying, embed to cutting. Thank you in advance. Thomas The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shandon Varistain Gemini Slide Stainer
Hi All,
Does anyone know the life expectancy of the Gemini H stainer? My boss has
asked me this question because we are in the process of requesting for a new
one.
Thomas
Thomas Huynh BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology
HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 |
thomas.hu...@harrishealth.org
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[Histonet] Decalcification
Hello Histoneters, I would like to add that washing after decalcifying is still not enough. The bone specimens have to be neutralized before processing ( putting them in the neutralizer solution), so there are no trace of acid when we process them. Thomas -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, July 20, 2015 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Decalcification (Steve McClain) 2. Cerner PathNet AP tracking (Younes, Pamela S) -- Message: 1 Date: Sun, 19 Jul 2015 19:00:38 + From: Steve McClain ste...@mcclainlab.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Decalcification Message-ID: d1120672-3c80-47aa-b578-85523b3c9...@mcclainlab.com Content-Type: text/plain; charset=us-ascii One frequently overlooked point is washing after decal. Especially important with acid decal, we wash for an hour in running water. -- Message: 2 Date: Mon, 20 Jul 2015 13:00:06 + From: Younes, Pamela S pamela.s.you...@uth.tmc.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Cerner PathNet AP tracking Message-ID: d9c344263273d74b870b35a54a88ba5a04367...@uthmail2.uthouston.edu Content-Type: text/plain; charset=iso-8859-1 Hello everyone, Has anyone used the Cerner PathNet AP tracking system? I would like to hear about any feedback, good or bad. Many thanks, Pam Younes Pamela S. Younes MHS, HTL(ASCP), CPC(AAPC), PA(ASCP) Pathologists' Assistant Assistant Professor, Department of Pathology and Laboratory Medicine University of Texas Medical School, Houston 6430 Fannin, MSB 2.233 Houston, TX 77030 (713) 704-2053 -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 140, Issue 22 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave Decalcification
Hi Kimberly, We are currently using the Milestone Medical brand microwave to decalcify our bone marrow core biopsies. After fixation we decal the core biopsies in the microwave for 2.5 hours at 50 degree Celsius with the 10% Formic Acid (we made up the formic acid in-house). If you need more info please email me. Thanks Thomas -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, May 22, 2015 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Microwave Decalcification (Kimberly Marshall) 2. Donating Old Microtome (Heather Marlatt) 3. Re: Donating Old Microtome (Liette Tougas) 4. Re: Donating Old Microtome (John Kiernan) 5. Buying service contracts (Johnson, Carole) 6. Re: Buying service contracts (Morken, Timothy) -- Message: 1 Date: Thu, 21 May 2015 17:25:04 + From: Kimberly Marshall kimbe...@animalreferencepathology.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave Decalcification Message-ID: 1432229164867.39...@animalreferencepathology.com Content-Type: text/plain; charset=iso-8859-1 ?Can anyone that has used the microwave decalcification method, please let me know how it worked for you? Thanks in advance. Kimberly Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.comhttp://www.animalreferencepathology.com/ Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] -- Message: 2 Date: Thu, 21 May 2015 12:49:48 -0700 From: Heather Marlatt hmarlat...@gmail.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Donating Old Microtome Message-ID: calavvk5jqsaf1b99eo0mxhjck4acgukqd+pkcnzsm_txzvn...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather -- Message: 3 Date: Fri, 22 May 2015 03:24:09 + From: Liette Tougas ltou...@dawsoncollege.qc.ca To: Heather Marlatt hmarlat...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Donating Old Microtome Message-ID: 455897b94cf3a44f81f727160f23fb11640c5...@dc229.ad.dawsoncollege.qc.ca Content-Type: text/plain; charset=iso-8859-1 Hi Heather, I do not know where you are located but we have 5-6 good old AO microtomes that still work very well that we are willing to donate. They are in excellent condition as they were extremely well maintained over the years and, as a Biomed Lab technology teaching department, they have been used only twice a week for 3 weeks each fall semester since they were purchased, some 30 years ago! They each have a disposable blade holder (high profile) and block clamp (vs original screwing block holder). We easily cut at 4 microns on them. The offer is opened to anyone who can pick them up or pay for shipment. Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montr?al, Qc, Canada 514-931-8731, ext 1519 From: Heather Marlatt [hmarlat...@gmail.com] Sent: May 21, 2015 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Donating Old Microtome I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather
[Histonet] Bone Station- Milestone
Hi Joana, We are currently using the Milestone microwave to decal our bone marrow core biopsies (for faster TAT). We use our in-house formic acid (10%) and run it in the microwave for 2.5 to 3.00 hours with 50 degree Celsius cycle (you can tweak to where it works for your specimens). Assuming that they have been fixed well prior to decalcifying; personally, I would rather decal them the old fashion overnight but they always rush them nowadays. Thomas -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, December 14, 2014 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. BoneSTATION - Milestone (Joana Moreira) -- Message: 1 Date: Sun, 14 Dec 2014 04:36:44 + From: Joana Moreira jmore...@sidra.org Subject: [Histonet] BoneSTATION - Milestone To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: a478ef23b3aad44cad6804356d5676bb02ebd...@mv3wexmx03prv.smrc.sidra.org Content-Type: text/plain; charset=iso-8859-1 Hi everyone! I was wondering if there's anyone out there using the Milestone BoneSTATION for fixing and decalcifying bone tissue: http://www.milestonemedsrl.com/histopathology/products/pre-analytical-tools/bonestation.html Is it good? Not good? Easy to operate and maintain? Do you use it with in-house decalcifying solutions or commercial available ones? Have you tried their MOL-Decal solution on BMT? Any thoughts? What about the optimization and standardization of protocols? Is it quite straight forward? Or complicated / Difficult? We are considering it and any feedback would be much appreciated. Send me a PM if you like. Many Thanks, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmore...@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 133, Issue 16 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Decalcifying and Processing large bone
Hi Bret We are using 10% Formic Acid as well and checking the decalcifying end-point by using the faxitron x-ray system. I work with bone cancer for 99% of the time and Formic Acid is safer than any other one. Thomas -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 06, 2014 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Protocol for decalcifying and processing large bone section. (DiCarlo, Margaret) 2. AW: [Histonet] RE: whole slide scanners (Gudrun Lang) 3. Glassware Cleaning, CAP Checklist Item GEN.41770 (Laurie Colbert) 4. RE: RE: whole slide scanners (Elizabeth Chlipala) 5. Listservers in microbiology (Jorge A. Santiago-Blay) 6. AW: [Histonet] RE: whole slide scanners (Gudrun Lang) 7. Procedures and Policy manual needed (Dr. Max Mirot) 8. RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Cooper, Brian) 9. RE: RE: whole slide scanners (Morken, Timothy) 10. RE: Listservers in microbiology (Cartun, Richard) 11. RE: RE: whole slide scanners (James Watson) 12. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Joelle Weaver) 13. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Jamal) 14. Re: Protocol for decalcifying and processing large bone section. (Barry Rittman) 15. Re: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Michael Ann Jones) 16. RI company seeks plastics company for research work (Hans B Snyder) 17. p16 (sarah.dys...@stdavids.com) 18. neutralize formalin (Mary Ann Deathridge) -- Message: 1 Date: Tue, 5 Aug 2014 17:01:16 + From: DiCarlo, Margaret mdica...@kaleidahealth.org Subject: RE: [Histonet] Protocol for decalcifying and processing large bonesection. To: 'Clough, Bret' clo...@medicine.tamhsc.edu, Histonet list serv. histonet@lists.utsouthwestern.edu Message-ID: 731be09cdb19aa43aa8682199d42d31b1dc79...@adcexchange01.kaleidahealth.org Content-Type: text/plain; charset=us-ascii I use 10% formic acid for large human bones but I don't think your bone sections are that large. Peggy DiCarlo Ortho Bone Lab BGMC 100 High St. Buffalo, NY 14203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret Sent: Tuesday, August 05, 2014 11:55 To: Histonet list serv. Subject: [Histonet] Protocol for decalcifying and processing large bone section. Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day. Any advise would be greatly appreciated! Sincerely, Bret Clough Texas AM Health Science Center Temple, TX. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are
[Histonet] Moh's Job
Hello everyone in the Histoland I am posting a job for a friend; they are looking for a fulltime Mohs' tech at a local dermatology practice in the Houston Texas Medical Center. Please contact Ms. Stephanie Huff at 281-799-0521. Thomas Thomas Huynh HT(ASCP) Dept. of Pathology Histology/ Bone Lab Tel. 713-745-4759 Fax. 713-792 2046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Powerpath QA
Ms. McCaig We are using it for quite some time; it is not the best out there but if you can contact me, I would be able to go into detail further. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, September 25, 2013 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 118, Issue 43 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Submitting Questions (Joanne Clark) 2. ER/PR results tracking (Clare Thornton) 3. RE: Submitting Questions (Nails, Felton) 4. Ceramide antibodies (Mark Elliott) 5. RE: RE: Submitting Questions (Douglas Porter) 6. Article: Anatomic Pathology Takes a Leap in Quality Process Improvement (Judy O'Rourke) 7. Re: Histonet Digest, Vol 118, Issue 42 (Mark Elliott) 8. RE: Submitting Questions (Martha Ward-Pathology) 9. RE: RE: Submitting Questions (pru...@ihctech.net) 10. PowerPath QA (Diana McCaig) 11. Ventana Vantage and PowerPath (Victor A. Tobias) 12. RE: ER/PR results tracking (Cartun, Richard) 13. RE: Peggy Wenks award (Tony Henwood (SCHN)) 14. Re: RE: Peggy Wenks award (David Kemler) 15. RE: edge effect on IHC (Tim Higgins) 16. Re: edge effect on IHC (Jan Shivers) 17. Re: RE: Peggy Wenks award (Jackie Lair) 18. Re: RE: Submitting Questions (nancy lowen) -- Message: 1 Date: Tue, 24 Sep 2013 17:53:56 + From: Joanne Clark jcl...@pcnm.com Subject: [Histonet] Submitting Questions To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 0494a7d4e8cc254ea2fb81464982e378b4aba...@s10maild001n3.sh10.lan Content-Type: text/plain; charset=us-ascii Can anyone out in histoland explain to me why my submitted questions are not making it through to histonet? I submitted a question on the 19th of September, and I still have not seen it appear in the group emails I get daily. I sent the question to the correct address (I checked it to be sure). When I respond to someones question, I can see my responses, I just can't seem to get an original question submitted. Can anyone tell me what I am doing wrong? Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. -- Message: 2 Date: Tue, 24 Sep 2013 14:40:15 -0400 From: Clare Thornton cthorn...@dahlchase.com Subject: [Histonet] ER/PR results tracking To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: c9d78ffc9d668b4cbea4405f84697504013d67ac1...@iris.dahlchase.net Content-Type: text/plain; charset=us-ascii How is everyone tracking their ER/PR results to comply with the following: ANP.22970 Annual Result Comparison Phase I For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The average is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The average is considerably lower in well-differentiated carcinomas (10%) and certain special types of invasive carcinomas (10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER in each of these settings. Investigation is warranted if the proportion of negative cases is significantly higher in any of these settings. Our LIS doesn't have an easy way of compiling these results. Basically, we are stuck with searching
[Histonet] RE: Decalcifying bone
Ms. Clark We are currently use 10% formic Acid in a microwave oven to decal ours for about 2 and 1/2 hour after they were fix in 10% neutral buffer Formalin. We were told that they are Ok for immuno-stains. Thomas Huynh, HT( ASCP) Clinical Histology Technician Pathology/ Histology/ Bone Lab thu...@mdanderson.org T 713-745-4759 F 713-792-2046 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, July 25, 2012 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 104, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Decalcifying bone (Jeanne Clark) 2. Re: RE: Secondary antibody causing nuclear staining (Eva Permaul) 3. Thank you for coverslipper responses (Brendal Finlay) 4. CAP Sample Exchange Registry (jgold...@bidmc.harvard.edu) 5. Re: Markers for Rat Samples (Hobbs, Carl) 6. Thanks for all the good wishes (Louise Renton) -- Message: 1 Date: Wed, 25 Jul 2012 07:14:06 -0700 (PDT) From: Jeanne Clark paintedsplas...@yahoo.com Subject: [Histonet] Decalcifying bone To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1343225646.39594.yahoomail...@web161703.mail.bf1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 In looking through old procedures, I have found several different 'solutions' that have been used for fixation and decalcification of bone (particularly bone marrow cores). ?I would very much appreciate hearing what people are using today for optimal fixation and decalcification of bone for routine pathology and IHC testing. Thank you, Jeanne Clark Histology/IHC Stanford Hospital and Clinics -- Message: 2 Date: Wed, 25 Jul 2012 10:59:58 -0400 From: Eva Permaul e...@georgetown.edu Subject: Re: [Histonet] RE: Secondary antibody causing nuclear staining To: Reynolds, Donna M dreyn...@mdanderson.org, histonet histonet@lists.utsouthwestern.edu Message-ID: CAEBNytUc2fe1o4Z2xv5AT_JFM46+c3Ya-g=xtgmneumntkh...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 I do see positive nuclei in the NC. That is what I am asking about. I know I could switch methods but my question is also why if it is happening is it not as strong all the time? Why are the cells very light one day and dark the next? What is causing them to stain? Just curious is all. Eva On Wed, Jul 25, 2012 at 10:04 AM, Reynolds,Donna M dreyn...@mdanderson.orgwrote: If you are running a negative control (no primary)with your ABC staining wouldn't you see the same nuclear labeling in the NC ? Thus alerting you to false staining and indicating that you should try a HRP conjugated secondary or use a polymer system. Good discussion thank Tony. Donna Reynolds HT(ASCP) Chief Histologist IHC Lab -Original Message- I understand the point about the biotin and I should have said that when using the ABC method we have taken to always using an avidin/biotin blocking kit. We are using biotinylated secondary antibodies from Vector. I have seen the same problem occur in our anti-mouse, anti-rabbit and anti-goat. In my last run I had stomach fundus as well as skin melanoma, both had pos.nuclei in the negative (no primary). In another run I had colon ca and breast ca, the breast ca had fewer pos. nuclei than the colon ca but they were still there. Some days the positive nuclei are stronger in a sample that was just weakly positive before. Just want to understand what it is and what effects it. Thank you all for your ideas. Eva Permaul Georgetown University On Mon, Jul 23, 2012 at 7:16 PM, Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au wrote: I should have added that this was from the workshop notes on a Hypotheticals Workshop I ran last year at our Australian National Meeting. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf
