Re: [Histonet] IHC validations

[Ingles Claire via Histonet]

Paula:
Usually CLIA follows CAP's lead or has less strict guidelines. Following the 
CAP guidelines should be good for both.
Claire

From: Paula via Histonet 
Sent: Tuesday, June 2, 2020 11:25 AM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] IHC validations

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Hello,

I see 10 positive and 10 negative cases for CAP guidelines, but what about
for CLIA?  What is their guideline to validate an IHC antibody?

Thanks in advance,

Paula

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Re: [Histonet] UV Light in Fume Hood

[Ingles Claire via Histonet]

UV-C wavelength is the best for the virus killing.
Claire

From: Paula via Histonet 
Sent: Thursday, May 14, 2020 1:28 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] UV Light in Fume Hood

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Hello everyone.. I hope all is well.



My admin wants me to look into possibly adding a UV light inside our fume
hood, which currently does not have one.  I'm thinking maybe it's not as
effective to kill viruses as we think it would be.



If anyone can share your thoughts about it, or if anyone has bought one and
has any insights to share, that would be greatly appreciated.



Have a great day and take care,

Paula

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Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

[Ingles Claire via Histonet]

OMG!!! really?!?
OSHA, Joint Commission, CAP, ANY regulatory agency. Give them the lounge 
microwave and get a new one for your break room immediately!! Fumes, 
contamination on the outside of whatever it's in, etc. Not even mentioning if 
it gets spilled.
But then again, our docs like to store Botox in our lunch fridge once in a 
while... *sigh*
Claire


From: Eileen Akemi Allison via Histonet 
Sent: Thursday, December 12, 2019 6:12 AM
To: Histonet 
Subject: [Histonet] guidelines for using a microwave designated for food, but 
used to heat up histogel

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Good morning histopeeps:

We recently brought on an Ophthalmology Pathologist from MD Anderson and they 
use histogel for orienting their thin eye specimens and need a microwave to 
heat it up.  We do not have a microwave in the lab for their use but I had 
ordered one for them.  I just found out they used the microwave in our lab 
lounge to heat up histonet which was opened and being stored in our gross area 
refrigerator.

Do any of you know the CAP regulation against use of laboratory reagents being 
used in a microwave being used for food consumption?  I ended up removing the 
microwave they used and put it in the histology lab.  Our Director wants me to 
write a formal letter to submit to him.

Thank you in advance for your impute!

Akemi Allison, BS,  HT/HTL (ASCP)


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Re: [Histonet] Flammable Sprays in Cryostats

[Ingles Claire via Histonet]

Liquid Nitrogen guns.
Claire

From: Knutson, Deanne via Histonet 
Sent: Wednesday, September 25, 2019 7:50 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Flammable Sprays in Cryostats

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I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.


Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org






  
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Re: [Histonet] Xylene substitutes for clearing agents

[Ingles Claire via Histonet]

Chris:
Propar from Anatech works great for us. I believe it is still advisable to use 
xylene in the cleaning cycle on the processors though. We had to go back the 
other way a bit when our Doc wanted a tape coverslipper. Now he gripes about 
the xylene smell. Hmmm.
Claire


From: Hagon, Christopher (Health) via Histonet 

Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Xylene substitutes for clearing agents

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UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the 
comparisons of each. I am charged with looking into converting our lab to go 
xylene free. We don't want to go down the limonene path, so that leaves the 
isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution 
(Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to 
change procedures. Was there much trial and error in changing the processing 
protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au



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Re: [Histonet] Softening Calf Hooves

[Ingles Claire via Histonet]

Sandra:
We here at the Dermatopathology department of
UW Mad have found:
-10% Potassium Hydroxide works best for us on our nails.

-We usually only need to have it in solution for a few hours. As you are trying 
to soften hoof, I might play with both concentration and time in solution.

- I don't believe Monty Python ever actually stated the relative air speed of 
either type of unladen swallow. 


Say HI to Vicki K for me.
Claire Ingles

From: Rene J Buesa via Histonet 
Sent: Thursday, March 21, 2019 12:32 PM
To: Sandra Cheasty; Histonet (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] Softening Calf Hooves

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Either potassium or sodium hydroxide will work at 10% aq. solution. Check it 
daily until soft enough.As to your "swallow" question, I "Goggled" your 
question and the answer is the following"
In the end, it's concluded that the airspeed velocity of a (European) unladen 
swallow is about 24 miles per hour or 11 meters per second. But, the real 
question is not about swallows at all. King Arthur in the movie had two coconut 
shells that he banged together to simulate the sound of a horse galloping.Jul 
7, 2013
René


On Thursday, March 21, 2019 12:06 PM, Sandra Cheasty via Histonet 
 wrote:


 Hello all,
I have 1 inch thick cross sections (band saw) of calf hooves. 
The pathologist has already decaled the bone, and now we have to soften the 
hooves so she can take thinner sections for processing.


*Which is better, potassium hydroxide or sodium hydroxide, and what 
concentration?

*How often should they be checked for softening?

*What is the relative air speed of an un-laden swallow? (European)

Thanks for your help!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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[Histonet] ST4020 Stainer

[Ingles Claire via Histonet]

Netters!
A question on behalf of a co-worker. We are in the process of validating the 
ST4020 stainer from Leica. We are a Mohs clinic that currently uses Carazzi's 
double strength Hematoxylin. We are looking for a better staining protocol. We 
are planning on moving to a Harris formula when switching so any Heme formula 
is possible right now.
Claire
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Re: [Histonet] Processing cystic tissue

[Ingles Claire via Histonet]

Charles:
I usually get out as much of the cystic material as I can without damaging the 
cyst wall itself. That is all the docs are looking at anyway. I am assuming you 
are referring to skin cysts, not ovaries, etc.

Claire


From: Charles Riley via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 04, 2017 9:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Processing cystic tissue

Can anyone give me an idea how they process cystic tissues. THe normal
tissue processes extremely well on my current protocol but the cystic areas
just are too soft to cut. Any tricks anyone can provide to process better
or cut better after processing would be greatly appreciated

--

Charles Riley HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Reference for this Recipe and Use

[Ingles Claire via Histonet]

Sadly, it is much safer than the old Chloroform clearing method.
Claire


From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Sunday, March 26, 2017 6:22 PM
To: ian bernard
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reference for this Recipe and Use

This is sometimes known as Newel's solution for revealing lymph nodes in fatty 
specimens:

Newell KJ, Sawka BW, Rudrick BF, Driman DK. (2001) "GEWF solution" Arch Pathol 
Lab Med 125:642 645.

Absolute ethanol1000ml
Water   340ml
Concentrated formalin (37%) 160ml
Glacial acetic acid 100ml



Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-Original Message-
From: ian bernard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Saturday, 25 March 2017 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Reference for this Recipe and Use

500 ML OF 100% ALCOHOL

170 ML OF DISTILLED WATER

80 ML OF 40% FORMALIN

50 ML OF GLACIAL ACETIC ACID



V/R

IB



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Re: [Histonet] Performing PAS with Diastase by Hand

[Ingles Claire via Histonet]

We get our Diastase powder from American Mastertech.

Claire
Dermatopathology lab
University of Wisconsin Hospital and Clinics


From: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, October 05, 2016 3:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Performing PAS with Diastase by Hand

We are starting to perform a PAS with diastase for fungus to be used by our 
dermatopathologist.   At first we weren't sure he was going to need a method 
that used diastase but he now prefers it.  Problem is our PAS counter kit did 
not come with diastase.  Please don't tell me to use saliva although I'm old 
enough to know we did for years.  We did order some powered diastase that was 
quite expensive and of course the working solution had a very short shelf-life. 
  Can anybody recommend a product and vendor that is relatively inexpensive and 
economical to use?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



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Re: [Histonet] Histonet Digest, Vol 151, Issue 1

[Ingles Claire via Histonet]

Not to mention that if you inhale too many fumes you will pass out. One of the 
original anesthetics. :)
Claire


From: Douglas Gregg via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, June 01, 2016 1:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 151, Issue 1

Subject: Re: [Histonet] Histology tips

Here is one that your safety officer will like. Ether is sometimes called
for as a de-stainer but is dangerous to keep except in a sealed can. Once
opened, it can be quite explosive in a frig. This is my solution. Get a
cheap can of engine starting fluid. It is pure ether. Don't get the high
priced version with lubricants added. You just use what you need from the
spray can and the rest keeps just fine for years.

Douglas Gregg DVM PhD
Veterinary pathologist
Southold, NY
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Re: [Histonet] PAS Stain

[Ingles Claire via Histonet]

I don't know, I believe Dr. Salk did the same with the polio vaccine. He even 
involved his family! Dedicated doctors...
Claire


From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 6:33 PM
To: Anne Murvosh
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

Only a crazy Aussie would do this!!

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


-Original Message-
From: Anne Murvosh via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, 6 May 2016 6:03 AM
To: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

You clearly don't know your histo history. The reason we know that H pylori 
exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria 
caused ulcers and not stress.  No one believed him.  So he took the organisms 
from a patient, mixed it in a broth and drank it. He then biopsied himself and 
treated it. There's a non-uniform method that saved a lot of suffering.  Bravo 
we crazy scientists.  Anne


-Original Message-
From: Geoff via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

I cannot believe any scientist would advocate such a non-uniform method as 
spitting on a slide.
Buy a bottle of what ever enzyme and use a reproducible buffer and temperature.

Geoff

On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote:
> Yes, spitting is the tried and true way to do it.  Not to mention no 
> measuring and cheaper.  The reason we switched to a powder is because I just 
> don't spit well I used to have someone do it for me cause I would end up 
> drooling. YUCK! The best way to find out is do the amylase method and the 
> spit method at the same time and have the doctor pick the best.  A fun 
> experiment  Anne
>
> -Original Message-
> From: Bob Richmond via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, May 05, 2016 11:36 AM
> To: koelli...@comcast.net
> Cc: Histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS Stain
>
> Spokane Ray points out something I've wondered about for years - can
> just anybody spit on the slide and remove the glycogen? I've never
> heard of any variation, but the number of people I've asked is very
> limited. This
> reference:
> http://www.ncbi.nlm.nih.gov/gene/276
> certainly suggests that different people have different salivary alpha
> amylase activity.
>
> Bob Richmond
>
> On Thu, May 5, 2016 at 2:27 PM,  wrote:
>
>> I love having the Samuri Pathologist on this forum for wisdom and
>> real-laboratory life knowledge.  And yes, I have in the past spit on
>> slide ON OCCASSION when faced with a dire necessity.  Although I know
>> there are those who would wretch about this; it remains a fact of
>> viable laboratory life for some.
>>
>> My problem now is that in this era of (MUCH TOO MUCH) regulation, how
>> do you "test lots" or control from "lot-to-lot variation" in this
>> SOP?  When Jane or Joe do this routinely and then goes on vacation,
>> what about Sally or Jim spit?  There is a variation in copy number of
>> the AMY1 gene
>> (amylase) and resulting difference in amylase protein concentration
>> amongst individuals.
>>
>> Why not just standardize it from the start, reagent, pH, temperature
>> and it really cannot fail.
>>
>> Spokane Ray
>>
>> --
>> *From: *"Bob Richmond via Histonet"
>> 
>> *To: *"Histonet@lists.utsouthwestern.edu" <
>> histonet@lists.utsouthwestern.edu>
>> *Sent: *Thursday, May 5, 2016 11:10:40 AM
>> *Subject: *Re: [Histonet] PAS Stain
>>
>>
>> Amylase (diastase) for the PAS stain queries:
>>
>> Whatever happened to spitting on the slide (30 min at room temperature)?
>> John Kiernan advises "thinking of lemons and drooling into a small beaker"
>> though I'd advise chewing on a rubber band for a few seconds.
>>
>> He notes that alpha amylase is preferred. I'd go with the cheapest
>> one in the Sigma-Aldrich catalog. Room temperature is usual, but I
>> note that Sigma offers a heat-stable alpha amylase.
>>
>> Bob Richmond
>> Samurai Pathologist
>> Maryville TN
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Re: [Histonet] PAS Stain

[Ingles Claire via Histonet]

The level of amylase in your saliva also depends on when you ate last. If 
you're spitting on slides after you just ate, the reaction will be weaker as 
the amylase will have been used up on lunch digestion. (also, you need to be 
careful about having your lunch salad contaminate the slide... :)
Claire


From: Ray via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, May 05, 2016 1:48 PM
To: Richmond, Bob
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Stain

An excellent point.  For anyone wanting to investigate-simply do a PubMed 
search on variation of AMY1 gene.  Sorry; I guess I should say this is, 
strictly speaking, non-histology related topic and I don't want to get into 
trouble as some before me.  Tons of research about this linking back (in 
theory) to positive selection in hunter-gatherers versus agricultural 
ancestors, race, obesity, phenotypic and dietary differences as to why maybe 
there can be big differences.
Spokane Ray

- Original Message -

From: "Bob Richmond" 
To: koelli...@comcast.net
Cc: "Histonet@lists.utsouthwestern.edu" 
Sent: Thursday, May 5, 2016 11:35:42 AM
Subject: Re: [Histonet] PAS Stain

Spokane Ray points out something I've wondered about for years - can just 
anybody spit on the slide and remove the glycogen? I've never heard of any 
variation, but the number of people I've asked is very limited. This reference:
http://www.ncbi.nlm.nih.gov/gene/276
certainly suggests that different people have different salivary alpha amylase 
activity.

Bob Richmond

On Thu, May 5, 2016 at 2:27 PM, < koelli...@comcast.net > wrote:



I love having the Samuri Pathologist on this forum for wisdom and 
real-laboratory life knowledge.  And yes, I have in the past spit on slide ON 
OCCASSION when faced with a dire necessity.  Although I know there are those 
who would wretch about this; it remains a fact of viable laboratory life for 
some.

My problem now is that in this era of (MUCH TOO MUCH) regulation, how do you 
"test lots" or control from "lot-to-lot variation" in this SOP?  When Jane or 
Joe do this routinely and then goes on vacation, what about Sally or Jim spit?  
There is a variation in copy number of the AMY1 gene (amylase) and resulting 
difference in amylase protein concentration amongst individuals.

Why not just standardize it from the start, reagent, pH, temperature and it 
really cannot fail.

Spokane Ray


From: "Bob Richmond via Histonet" < histonet@lists.utsouthwestern.edu >
To: " Histonet@lists.utsouthwestern.edu " < histonet@lists.utsouthwestern.edu >
Sent: Thursday, May 5, 2016 11:10:40 AM
Subject: Re: [Histonet] PAS Stain


Amylase (diastase) for the PAS stain queries:

Whatever happened to spitting on the slide (30 min at room temperature)?
John Kiernan advises "thinking of lemons and drooling into a small beaker"
though I'd advise chewing on a rubber band for a few seconds.

He notes that alpha amylase is preferred. I'd go with the cheapest one in
the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
offers a heat-stable alpha amylase.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] Running a histo lab without a histotech?

[Ingles Claire via Histonet]

Not to my knowledge. We have 3 derm/Mohs labs here in our system. There are 
only two of us who have our certifications. Heaven knows the world (or the lab 
for that matter) doesn't stop revolving when WE go on vacation. The third lab 
is a one person show and she isn't certified. Just so all the CLIA/JCHO/CAP 
stuff is being adhered to. The only thing I can think of regarding 
training/college must haves are for grossing as it is considered high 
complexity. I agree with Rene. Not your problem.
Claire


From: Rene J Buesa via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, October 23, 2015 7:35 AM
To: Jay Lundgren; histonet
Subject: Re: [Histonet] Running a histo lab without a histotech?

About the "legality" I do not think it is of your direct concern, unless you 
want to "challenge" the situation with some sort of legal action, which is 
really not advisable.As a functioning laboratory in a hospital it has to be 
under the supervision of either CLIA or  ASAP and the pathologist is the 
Director of such laboratory so, it is the pathologist's responsibility of 
assuming about the "legality" of that situation and is the pathology who you 
should ask this question.René


 On Thursday, October 22, 2015 5:02 PM, Jay Lundgren via Histonet 
 wrote:


 Hello histonetters!  I am on assignment in a small hospital lab in the
Heartland and they are staffed by only two people.  One is a registered
(ASCP) HT with 10 years of experience, and the other is a former
phlebotomist being trained on the job from scratch , who has yet to take
her accreditation exam.

 As a matter of fact, she has yet to take all the college courses (math,
science) to qualify to take her exam, and is still being  trained in the
hospital's protocols, only allowed to cut larger specimens (no bxs), etc.
She can accession, answer the phone, embed, load the stainer, coverslipper,
etc.,  so she can turn out most of the work.  I am here working for three
weeks because the trainee is on medical leave.

My question is, when the registered HT goes on vacation or sick leave, is
it legal for the lab to operate with only the trainee?

Or does a histo lab have to have at least one registered tech to operate?


  Thanks,

  Jay A. Lundgren, M.S., HTL (ASCP)
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Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

[Ingles Claire via Histonet]

We have had this problem for years. We are more than willing to train someone. 
We are a small lab so a bigger concern is if they will fit in with the rest of 
us personality wise. Not that we just hire anyone willy-nilly, but many people 
with BS's have some lab experience/background anyway. We just make sure they 
have at least a BS so they fit the qualifications to gross. I am actually the 
only one in a lab of 4 with my certification, and though I also have an AA in 
Histotechnology, my BS in Biology made getting the AA alot easier. It is more 
of a concern if they can't troubleshoot and adapt on the fly to the ever 
changing situations in the lab. We actually just hired an HT with 28 years of 
experience, but don't know if she is going to make it past her 6 month eval. 
She can't multitask and has a hard time adapting to the different specimen 
types. I think she has worked mostly in research in the past. (not belittling 
you researchers out there, but many of you know that research vs. cli
 nical work can take different skill sets.)
Claire


From: Michael LaFriniere via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Monday, August 10, 2015 1:39 PM
To: tgen...@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

Yes this is a common problem, although I am not sure desperate enough would 
be appropriate  verbiage.  As histologists over the years we have learned to 
cope with and being highly resourceful. I have also resorted to the same idea, 
hiring both AS and BS individuals filling positions and training specific 
technical task to meet the production needs at present has worked very well in 
my laboratory.

Michael
Michael R. LaFriniere, HT (ASCP)
Executive Director


Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844
michael.lafrini...@ccplab.com



-Original Message-
From: Tyrone Genade via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, August 10, 2015 12:13 PM
To: histonet
Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate?

Hello,

Last week a I visited Pittsburgh and had a chance to talk with a fellow 
histologist there. He remarked on the dire state his lab is in with respect to 
finding qualified histologists to employ; and that the lab was now considering 
hiring B.Sc. graduates and training them up in sectioning etc... Is this a 
common problem, the trouble finding qualified histotechs, and are other labs 
also considering hiring B.Sc. graduates to staff their labs?

I have one student working with me (with medical school aspirations) who 
sections beautifully. There is surely talent out there...

Bye
--
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.
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Re: [Histonet] Manual Cassette Labelling

[Ingles Claire via Histonet]

We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # 
is 14-905-30 for black, 14-905-35 for red. We have never had a problem with 
them smearing or fading on the cassettes no matter what we subject them to.
Claire


From: Joanne Clark via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Thursday, August 06, 2015 3:27 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Manual Cassette Labelling

Hi Histoland,

We have a satellite lab that manually prepares their cassettes.  We have had 
significant problems with the markers coming off after tissue processing.  We 
have tried StatLab markers, Leica Markers and the red AquaRellable pencils and 
continue to have issues.  Other than purchasing a cassette labeling system 
(their volumes are pretty low and there is limited bench space in the lab for a 
cassette labeler) does anyone have any suggestions or ideas on what might be 
causing this phenomenon or a really good permanent lab marker that has been 
successful for you?

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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Re: [Histonet] Marciafava-Bignami Disease

[Ingles Claire via Histonet]

I know I watch my exposure to Formalin and Xylene especially. I was diagnosed 
with NASH in 2007 (who knows how long before then, since I am asymptomatic) 
when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) 
Anyway, I have always wondered about the cause. I thought it was genetic as my 
father also had it for over 40 years before finally having a transplant. But I 
wonder if it was his exposure to jet fuel, etc. as he was a jet mechanic for 20 
years in the USAF plus another 15-20 with a private company. So far my liver 
has been stable even getting a bit better to the point of being just a hair 
above the normal range. I do everything under the sun in my lab. Mohs, 
Grossing, plus all the routine stuff of cutting and staining, etc. I have been 
able so far to keep the repetitive injuries at bay, and Dansco shoes have saved 
my feet from the Plantar Faciitis (had that about a year straight at one 
point.). We had managed to keep xylene out of our lab for years by usi
 ng Propar, but one of the Pathologists insisted on having a tape coverslipper. 
We still use it as little as possible. I had a co-worker, when she was pregnant 
wasn't allowed to gross (hence contact with formalin). Because of the NASH I 
get blood tests done every 6 months. It will be interesting to see if they have 
changed possibly due to the exposure to xylene, which I haven't had exposure to 
for years otherwise.
Claire


From: Mayer,Toysha N via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, August 04, 2015 2:49 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Marciafava-Bignami Disease

This is very interesting.  I have worked in the field for over 20 yrs, and was 
pregnant while working in a small lab.  Mostly everything was manual, and I did 
not use all of the safety precautions I should have (my fault). My son had 
severe speech and language delay as well as a language processing issue when he 
was smaller, and now stutters.  I have often wondered (as most parents would) 
if my lack of precautions may have contributed. We stress the safety issues to 
the students daily, to get it all in their heads.  I often tell  people that I 
am so happy because I work with xylene, and I feel real good.  So far, I don't 
see any noticeable symptoms of anything wrong, just the usual-decreased 
olfactory sensing, repetitive motion issues, plantar fasciitis, and varicose 
veins.  Hair loss was an issue at one time, but that was attributed to PCOS.  
Now that I am not in the lab full time anymore it has decreased since I went 
natural (I'm African-american).  One thing I have noticed is my C
 -reactive protein is high, and I take a statin for that.  I have no liver 
issues, and my functions appear to be normal.  Next time I go in for a 
physical, I will have my workplace hazards documented in my EMR.
I will look up Primary Biliary Cirrhosis (PBC), to get some more information, 
especially since I was born jaundiced and I am anemic.


Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481


Message: 11
Date: Tue, 4 Aug 2015 06:30:12 -0700
From: Eileen Akemi Allison akemiat3...@gmail.com
To: Edmondson David (RBV) NHS Christie Tr
david.edmond...@christie.nhs.uk
Cc: Histonet histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged
into thesystem and maybe this will now communicate with 
Histologists
out there,  and hope that I do not get twice as many emails from
Histonet#
Message-ID: e3d7c5a9-c516-4f0e-b522-61b348820...@gmail.com
Content-Type: text/plain;   charset=utf-8

GP?s are fine for general health issues, but I would certainly get more 
conclusive tests done by a neurologist, as well as contacting the best worker?s 
comp attorney in your area who has dealt with chemical exposure cases.

Studies show we are #1 in this country for the most hazardous professions. It's 
safe compared to when I 1st started in this field in 1965! Well, I am a 
dinosaur from back in the day when we had inadequate ventilation, a shortage of 
fume hoods, inadequate education on hazards, safety and PPE's.

In 1979 I started to work at OHSU in the surgical path lab, as well as doing 
research projects. We made up all of our own HE's and special stains from 
scratch, as well as made up our own 10% NBF in 55 gallon drums without fume 
hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate 
embedded tissues without hoods or gloves! Since it was a medical school, we did 
every special stain under the sun and dealt with about every chemical, reagent, 
acid and stain you could think of! We also smoked cigarettes and drank coffee 
in the lab while we embedded and cut! We sure were a naive group back then!

In the early days, the facilities I