[Histonet] Histology Supervisor Position
Clinical Laboratory of the Black Hills is an independent pathology practice providing anatomic and cytologic services to Rapid City, South Dakota and surrounding communities. Rapid City is the gateway to the Black Hills and offers a variety of four season, family friendly activities. Our histology department processes 25,000 surgical cases, and 200 autopsies per year. We also have a progressive IHC department, and perform a variety of special stains and frozen sections. HISTOLOGY SUPERVISOR Candidate must demonstrate strong leadership skills in areas of developing, training, and motivating staff. Requirements are HT/HTL certification and 2 years of successful management experience. Functions include oversight, supervision, and coordination of all histology activities for a department of 9 employees. Assists Operations Manager in developing, implementing, revising, interpreting, and enforcing standard operating procedures, company policies, and service standards. Assists with purchasing and budgeting for the histology department. Participates on the quality assurance committee. Oversees scheduling, employee time off requests, and evaluations. Helps maintain turnaround time expectations and performs other tasks as assigned. Clinical Lab offers a competitive wage with an excellent benefit package including health and dental insurance, 401(k), Profit Sharing, and long-term disability insurance. No state income tax. Relocation assistance available. Send resume to: Janet Amundson, Human Resources Clinical Laboratory of the Black Hills 2805 5th Street, Suite 210 Rapid City, South Dakota 57701 Fax: 605-342-0418; Phone: 605-343-2267 Email: jamund...@clinlab.com Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills Main: 605-343-2267 Direct: 605-716-4206 jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com> www.clinlab.com <http://www.clinlab.com> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16 Antibody
We are looking to bring in P16 to do in house but are having a hard time getting the stain to be clean. We are having issues with positive staining on tissue that should be negative. We are working with a concentrated antibody that we have diluted out to 1:6000 (which is 6 times the amount suggested from vendors 1:200-1:1000). We might need to change clones or vendors and were wondering if you could tell us what kind of p16 (clone) you use for staining and possible what vendor you get it from? Any information or suggestions you have would greatly be appreciated. Thank you. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com www.clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GROSSING UNDER DIRECT SUPERVISION OF A PATHOLOGIST
Here are the CAP Regulations: ANP.11605 Gross Examination - Non-Pathologist Phase II When individuals other than a pathologist or pathology resident assist in gross examinations, the extent of their activities and the nature of supervision (direct vs. indirect) is defined in a written protocol. NOTE: This protocol must list the specific types of specimens for which non-pathologists are permitted to assist in the gross examination. The nature of the supervision must be established individually, for each non-pathologist. The laboratory director is responsible for this protocol. For Mohs surgery a dermatologist is also qualified to perform the gross examination and to supervise non-pathologists. REFERENCES 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 1992(Feb 28):7183 [42CFR493.1489(b)(7)] 2) Cibull ML. Q Northfield, IL: College of American Pathologists CAP Today. 1997;11(7):112 3) Grzybicki DM, et al. National practice characteristics and utilization of pathologists' assistants. Arch Pathol Lab Med. 2001;125:905-912 ANP.11610 Gross Examination Qualifications Phase II If individuals other than a pathologist or pathology resident assist in gross examinations, such individuals qualify as high complexity testing personnel under CLIA regulations. NOTE: Grossing is defined as a tissue examination requiring judgment and knowledge of anatomy. This includes the dissection of the specimen, selection of tissue, and any level of examination/description of the tissue including color, weight, measurement or other characteristics of the tissue. The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under the CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a chemical or biological science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes the following: ● 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes six semester hours of chemistry, six semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination, AND ● Laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least three months of recorded laboratory training in each specialty in which the individual performs high complexity testing. It is the responsibility of the laboratory director to determine whether an individual's education,training and experience satisfy the requirements of this checklist requirement. This checklist requirement applies only to laboratories subject to US regulations. Evidence of Compliance: ✓ Records of qualifications including degree or transcript and work history in related field REFERENCES 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072 [42CFR493.1491] ANP.11640 Competency Assessment of Non-Pathologists Phase II The competency of non-pathologist(s) who assist in the performance of gross tissue examinations is assessed by the pathologist at least annually. NOTE: Please refer to GEN.55500, Competency Assessment, in the Laboratory General checklist for a list of criteria and frequency for competency assessment. Not all six elements may apply in all cases. For Mohs surgery a dermatologist is also qualified to perform the gross examination and evaluate non-pathologists. Evidence of Compliance: ✓ Written procedure and schedule for assessing competency of non-pathologists AND ✓ Records of competency assessment performed at a defined frequency REFERENCES 1) Cibull ML. Q Northfield, IL: College of American Pathologists CAP Today. 1997;11(7):112 2) Grzybicki DM, et al. The usefulness of pathologists' assistants. Am J Clin Pathol. 1999;112:619-626 3) Galvis CO, et al. Pathologists' assistants practice. A measurement of performance. Am J Clin Pathol. 2001;116:816-822 Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com> www.clinlab.com <http://www.clinlab.com> -Original message- > From:Patti Nelson - PN Lab Consultant via Histonet > <histonet@lists.utsouthwestern
[Histonet] Breast Her2Neu IHC vs FISH
Does anyone know or can you point me in the right direction to some literature about how to properly test for Her2Neu (IHC vs. FISH) on breast tumors if the cold ischemia time is greater than 1 hour or if the formalin fixation times are outside of the recommended time range? Also, what if the specimen has been placed in decal? We are struggling to find any documentation of how to properly test Her2Neu if the specimen is outside of these ranges. Thanks for your help! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com> www.clinlab.com <http://www.clinlab.com> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recycled reagents in VIP processor
Here is our experience with recycled reagents: We have been recycling Xylene and Alcohol since 12-2008. It can work well for you and save you money. It was a challenge to put it in play where it made the recycling program a good thing. We found out that the theoretical and the reality of recycling are not the same thing and you may need to "find" a way to make it work. The first truth about recycling is that you can recycle and retain a large portion of your alcohol but with special considerations. The recycler that we purchased will only recover a purity quality of about 95-97% alcohol back in product. That meant that we were stock piling huge volumes of 95% and still required to purchase 100% vendor grade for most processes. This was a nightmare event for us. I got cleaver and measured the alcohol percentages of our processors (the biggest consumer of alcohols) and found that the actual values in the 100% alcohol (x4) stations were closer to 94%, 96%, 97%, 99% (an alcohol hydrometer was used for this measurement). I then realized that I could use the 95% alcohol in the processors to use it up. I modified the preventative maintenance to change out all of the first 3 alcohols to 95% recycled alcohol and the very last one remains at 100% vendor grade alcohol, we also do not keep any alcohol that is not at a 95% value or higher. any 70% alcohol is just discarded at change out. (before this recycler plan our preventative maintenance plan was to use the reagents in the processor for 3 weeks by a bump-&-dump method; the first reagent in a series would be dumped each week and all of the reagents of that series after that station were moved (bumped) down into the station prior, then last station was always new 100% vendor grade reagent. This allowed us to "use up" the reagents and not replace them to often. This also retains a certain amount of consistency within the processing system so microtomy doesn't' experience changes when maintenance is performed on the processors.) Along with this processor modification I adjusted the changing of all these 95% alcohols off the instrument twice a week to be sure that they didn't drop too low and affect processing quality, the last station still remains at 100% vendor grade reagent. I would be glad to give you more details on how this worked for us if you would like to hear more about it or have my help to set up your program. One of my thinking processes for recycling program is to change out the reagents as often as you can/need/ or want to. to keep the quality high enough for the function it is used for. Remember that you are taking it out of use often but not throwing it away. The conservation of your reagents, and dollars, lies in the repeated use of the same reagents so you do not have to be "stingy" with the preventative maintenance changes. other thoughts; you still need to purchase 100% vendor grade reagents for some processes, but that is OK, because that becomes a way to bolster up your recycled quality and fight off the reality of diminishing returns. Recycling also decreases your waste being hauled off so it decreases the dollars in waste removal. The recycling of Xylene is excellent and we get a purity return of about 99.9% back on every recycling run. Once we started the recycling and had a program in place that actually worked for us, and with us, we were operating at a cost of about 30% of the prior operational cost for our Alcohol and Xylene. We currently are operating at about 36.6% of the reagent costs for Alcohol and Xylene with todays pricing and our current volumes when compared to the old method of "dump-&-bump" pm schedules. We have 4 tissue processors and 2 stainers in use regularly. Our workload volumes allow this to work for us and save us a great deal of money. You are welcome to call me and get answers to any questions you may have. Scott Johnson, HTL (ASCP) Histology Supervisor Clinical Laboratory of the Black Hills 605-343-2267 Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com> www.clinlab.com <http://www.clinlab.com> -Original message- > From:Gareth Davis via Histonet <histonet@lists.utsouthwestern.edu > <mailto:histonet@lists.utsouthwestern.edu> > > Sent: Wednesday, February 22, 2017 1:42 PM > To: histonet@lists.utsouthwestern.edu > <mailto:histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Recycled reagents in VIP processor > > Hi, > I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in > processors. I am using a VIP 300, refurbished, and I would rather not use > recycled reagents in it. But, during the last CAP inspection they > suggested I use the recycled to save money. An
[Histonet] Cassette Printers
We have been using a Leica IP-C cassette printer for the past 8 years and have experienced some issues this past year with it. Anybody know what the life expectancy is of this cassette printer? What other cassette printers work well? Thank you in advance for your response. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com <mailto:jmcgo...@clinlab.com> www.clinlab.com <http://www.clinlab.com> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS Control
We are in need of a GMS control for fungus. We have an excellent AFB control to trade. Please contact me if you are able to help. Thanks. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old slides.
Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Bernice Frederick b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu Sent: Monday, March 9, 2015 1:51 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bone Saw
We use a Dremel tool. It works great!! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Mike Pence mpe...@grhs.net mailto:mpe...@grhs.net Sent: Tuesday, February 17, 2015 1:56 PM To: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu ; histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Saw I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking small samples
Eosin in the alcohol works great to mark these small pieces of tissue. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Yves Heremans yves.herem...@vub.ac.be mailto:yves.herem...@vub.ac.be Sent: Wednesday, January 14, 2015 3:11 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech position opening
Clinical Laboratory of the Black Hills is a growing, independent pathology practice providing anatomic and cytologic services to Rapid City, South Dakota and surrounding communities. Rapid City is the gateway to the Black Hills and offers a variety of four season, family friendly activities. Our histology department processes 25,000 surgical cases, and 200 autopsies per year. We also have a progressive IHC department, and perform a variety of special stains and frozen sections. HISTOTECH Immediate opening for a certified HT/HTL (ASCP) or equivalent. Duties include embedding, microtomy, chemical and reagent management and IHC procedures. Associate Degree in related field a plus. F/T – Day shifts only. Clinical Lab offers a competitive wage with an excellent benefit t package including health, vision and dental insurance, 401(k), Profit Sharing, and disability insurance. No state income tax. Relocation assistance is available. Send resume to: Janet Amundson, Human Resources Clinical Laboratory of the Black Hills 2805 5th Street, Suite 210 Rapid City, South Dakota 57701 Fax: 605-342-0418; Phone: 605-343-2267 Email: jamund...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leased tech to GI practice
I am wondering if any anatomic pathology labs out there have leased a FTE, histology tech, to a GI practice to run a histology lab in the Endoscopy clinic? How did you logistically accomplish this? You can contact me offline for more details. Thank you in advance for your responses. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Pharm DX Kit for ER/PR
We are wondering how many labs are using Dako's PharmDX kit for their ER/PR's. Do you find it reliable? Do you have many repeats? Thank you for your responses. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Humidity levels and IHC staining
We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Humidity levels and IHC staining
We use the Dako Autostainer. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -Original Message- From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Friday, February 17, 2012 12:20 PM To: histonet@lists.utsouthwestern.edu; Jason McGough Subject: Re: [Histonet] Humidity levels and IHC staining A good auto stainer (like DAKO) with adequate amounts of dispensed reagents during the correct periods of time should not experiment any drying out on the slides. Adequate humidity is required to be controlled during manual IHC, especially if done over a heated support. If because of any reason (including not leveled slides) you experiment drying out, the best way would be to have an open flat dish containing water but, again, that was never a problem for me using the DAKO auto stainer. Which auto stainer are you using? René J. --- On Fri, 2/17/12, Jason McGough jmcgo...@clinlab.com wrote: From: Jason McGough jmcgo...@clinlab.com Subject: [Histonet] Humidity levels and IHC staining To: histonet@lists.utsouthwestern.edu Date: Friday, February 17, 2012, 2:09 PM We are wondering what other labs are doing to control the humidity while IHC stains are being performed. We currently place wet towels and a small weigh boat with water in our Autostainer to help prevent our slides from drying out but that seems to not be enough, they still tend to dry out and produce background staining. What should the humidity level be at? Any help would be appreciated. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Factor XIIIa background staining problems
We have been experiencing Factor XIIIa background staining problems and cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa clone EP3372 at a dilution of 1:250. We have tried many different protocols (i.e. enzyme pretreatment, high and low pH pretreatment, different incubation times, etc.)and nothing seems to fix this background staining. Our vendor told us that the dilution is good but we are questioning the actual dilution or the shelf life of the antibody. The antibody is a long ways from reaching the expiration date but we are still questioning it's stability. Has anybody else experienced this or know a solution? Thanks, Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura Film Removal
We soak the slides in acetone for 5-10 minutes and the film will peel off very easily. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Paula Lucas Sent: Thursday, July 29, 2010 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Film Removal Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon because the section was starting to lift off with the film. I appreciate the help Paula Lucas Lab Manager Bio-Path Medical Group FV, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AB-PAS for Barrett's Esophagus
1% Periodic Acid Solution 0.5% Sodium Metabisulfite Periodic Acid1 gm Sodium metabisulfite.0.5 gm Distilled water..100 ml Distilled water100 ml .1N HCL 3% Glacial Acetic Acid Conc. (10N) HCL.1 ml Conc. Glacial Acetic Acid..3 ml Distilled Water.100 ml Distilled water..100 ml Alcian Blue Solution pH 1.0Alcian Blue Solution pH 2.5 Alcian Blue 8GX...1.0 gm Alcian Blue 8GX1.0 gm 0.1N HCL100 ml 3% Glacial Acetic Acid.100 ml Adjust pH to 2.5. Add a few Schiff's Reagent Thymol crystals for preservative. Purchased Commercially (ready to use) PROCEDURAL NOTES 1. pH 2.5 is routinely used in our laboratory. Use pH 1.0 only if the pathologist specifically requests you to do so. 2. Filter Alcian Blue solutions before use. 3. Use an aliquot from the stock bottle of Schiff's reagent. This may be reused but do not return to stock bottle. Change frequently and discard if solution is pink. PROCEDURE 1. Deparaffinize and hydrate to distilled water. 2. Place slides in freshly filtered Alcian Blue solution for 30 minutes. 3. If using Alcian Blue solution 2.5 - Wash in running water for 5 minutes. If using Alcian Blue solution 1.0 - Blot section dry with fine filter paper. 4. Oxidize in Periodic Acid solution for 10 minutes. 5. Wash in running water for 5 minutes. 6. Place slides in Schiff's reagent for 10 minutes. 7. Rinse in Sodium Metabisulfite solution, 3 changes; 2 minutes each. 8. Wash in running water for 10 minutes. 9. Dehydrate in 95% alcohol, 100% alcohol, clear in xylene, two changes each. 10. Coverslip. RESULTS 1. pH 2.5 (acid group) -Exclusively acid substances (various connective tissue mucins) - blue -Neutral polysaccharides (glycogen) - magenta -Both Alcian blue and PAS, yielding varying shades of purple to deep blue, color most epithelial mucins and cartilage ground substance. -Cell bodies of fungi - red to purple -Mucoid capsules - blue -Other features resemble PAS stain 2. pH 1.0 (sulphate group) Sulfated mucosubstances - blue REFERENCE 1. Lev, R., Spicer, S.S.;J. Histochem. Cystochem. 12:309,1964. Copyright by Williams and Wilkins Co. 2. C.F.A. Culling; Handbook of Histopathological and Histochemical Techniques, 3rh e. 1974. Butterworth. 3. Preece, Ann: Manual for Histologic Technicians 2nd ed. 1965. 4. AFIP Manual For Histologic Stain Methods, 3rd ed. 5. Schenk, E.A., M.D. and Mowry, R.W., MDD, Journal of Histotechnology Vol. 6#2, June 1983. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana McCaig Sent: Thursday, June 24, 2010 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB-PAS for Barrett's Esophagus Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CMV Validation Slides
We are wondering if anybody can help us with our CMV validation. We are needing 1 slide from 20 different known positive tissues. If you are able to help us or know of a source, please reply back to me. Thank you in advance for your help. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS Fungus control
I wondering if anybody out there would be willing to trade GMS fungus controls for AFB controls. Please contact me if interested. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Validation of IHC
Our lab is inquiring about how other labs are validating their IHC stains. We currently are processing specimens both in a microwave and conventional processors. Are labs validating every type of program on the conventional and microwave processors? (i.e. small biopsies vs. larger tissue samples)Or just microwave vs. conventional processing? Also how many blocks of each tissue control are you testing? Thanks in advance for your replies. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Not False! Cell phone numbers go public next month
You can register your cell phone but there is no risk of your number going to telemarketers if you don't. If you go to www.snopes.com you can read more about it. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Akemi Allison-Tacha Sent: Friday, June 12, 2009 9:14 AM To: Peter Carroll Cc: histonet Subject: Re: [Histonet] Not False! Cell phone numbers go public next month Hi All, This is not false information I just called the government number from my cell and registered. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com --- On Fri, 6/12/09, Peter Carroll carro...@umdnj.edu wrote: From: Peter Carroll carro...@umdnj.edu Subject: Re: [Histonet] Cell phone numbers go public next month To: Akemi Allison-Tacha akemiat3...@yahoo.com Cc: histonet histonet@lists.utsouthwestern.edu Date: Friday, June 12, 2009, 8:06 AM This is false information. I really wish people would take half a second to verify the false rumors they're helping spread around the internet before sending this tripe to everyone else... especially as an off-topic post spammed to a public mailing-list. Read more about the truth behind this rumor: http://www.snopes.com/politics/business/cell411.asp Akemi Allison-Tacha wrote: Hi All, Happy Friday! This is a little off the histology subject, but for those of you who have cell phones, might find this beneficial. Akemi Allison-Tacha BS, HT (ASCP) HTL Histology Manager Associated Pathology Medical Group Laboratories 105A Cooper Ct. Los Gatos, CA 95032Direct: 408.884.2718 Cell: 408.335.9994 W: E-Mail: aallison-ta...@apmglab.com P: E-Mail: akemiat3...@yahoo.com FYI Folks! REMEMBER: Cell Phone Numbers Go Public next month. All cell phone numbers are being released to telemarketing companies and you will start to receive sales calls. YOU WILL BE CHARGED FOR THESE CALLS Even if the message is saved on your phone, you will be charged for the minutes to listen to it. To prevent this, call the following number from your cell phone: 888-382-1222 It is the National DO NOT CALL list. It will only take a minute of your time. It blocks your number for five (5) years. You must call from the cell phone number you want to have blocked. You cannot call from a different phone number. HELP OTHERS BY PASSING THIS ON TO ALL YOUR FRIENDS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura film coverslipper xylene residue issue
It has nothing to do with your coverslipper. Ours is from the mid 90's and we have had the brown spots a couple of times. We found that there to be water in our xylene. The little water droplets on the side of a container will cause this. Make sure everything that holds xylene is completely dry. Once we did this, we had no more brown spots. Good luck! As far as xylene residue, it is your dispensing volume on the coverslipper that is causing this. Try turning the flow down and see what happens. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgo...@clinlab.com Quoting Jackie M O'Connor Jackie.O'con...@abbott.com: I have an older Sakura coverslipper, circa 1997ish. We have noticed an increasing number of brown spots on tissue sections recently. This does not occur if the same slides are covered with glass coverslips. It does not look like a drying artefact, occurs randomly throughout tissue types, location on tissue, i.e., edge, center. I've even showed these spots to the rep who admitted she has never seen this artefact before. We use only the Tissue Tek tape, we maintain the equipment on a regular basis. The xylene flow is good. Our only problem are these random brown spots. Any suggestions? Jackie O' Karin Groeger kgroe...@uslabs.net Sent by: histonet-boun...@lists.utsouthwestern.edu 05/29/2009 08:19 AM To Scott lsc...@sfcn.org, Histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Sakura film coverslipper xylene residue issue Hi Scott, We place ours in front of a fan to dry if we are in a hurry, even if we air dry them we do not have a problem with the xylene residue, perhaps you have a bad batch, check your lot # and call Sakura. We coverslip over 1,000 slides a day and have not seen this. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott Sent: Thursday, May 28, 2009 10:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura film coverslipper xylene residue issue Hi, We have a newer Sakura film coverslipper, does anyone have experience with this piece of equipment. I have a problem with the slides having a light xylene residue, after the slides come off of the coverslipper. How do you dry them? If I leave the slides on the coverslipper to air dry the xylene residue is noticeable. If I lightly wipe off all of the slides as they come off the coverslipper they look clean. How do you guys do it? I think it is a waste of time to wipe off all of the slides. I don't think the xylene flow is too high. Thanks for your help, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reusing Citrate Antigen Retrieval Buffer
We are looking into using our .01M Citrate Antigen Retrieval Buffer in our Dako PT Link for Antigen Retrieval on our IHC stains. The question that we are wondering about is anybody reusing this solution for multiple times? If so, how many times? What is your dilution? Like the High pH Antigen Retrieval solution from Dako, it is recommended to use up to 3 time before replacement. We are having several problems with the High pH antigen retrieval solution from Dako and want to try Citrate, since that is what we have been using for many years with great results. We previously used the Pascal Pressure Cooker from Dako for Antigen Retrieval but now have the PT Link. Thank you in advance for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protein Block
We recently purchased a Dako Autostainer with the PT Link for our IHC's. We are experiencing more background staining then before when we had the older version of the Dako Autostainer. Does anybody use protein block (serum free) to help minimize background staining? Thank you for your replies. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
