Re: [Histonet] Antibody diluent

[Jeanine Ronkowski via Histonet]

Scott:I’m pretty certain that you wouldn’t need to completely re-validate after 
changing diluents, but you may have to change the dilution factor slightly.  
It’s my understanding that a change like this only requires staining a few 
known positive samples, verifying that the results you obtain are very similar 
if not identical to previous results.  While taking someone else’s suggestion 
to download that paper on diluents from Biocare’s site, I came across a paper 
on the subject of validation/verification that I found interesting.Jeanine


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On Thursday, March 25, 2021, 8:30 AM, Lindrud, Scott 
 wrote:

What is everyone's take on what needs to be done from a verification/validation 
standpoint if you switch diluents for your concentrated antibodies?  The CAP 
summary of recommendations on the validation IHC antibodies doesn't 
specifically mention the changing of diluent as one of the variables.

If I change the diluent on my ER and Her2 concentrates, do I need to do a 
complete re-validation or just a quick verification using a few positive and 
negative cases?  I'm not really liking the idea of a complete re-validation!

Thanks for any info!

Scott


Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201




-Original Message-
From: Jeanine Ronkowski 
Sent: Tuesday, March 23, 2021 2:21 PM
To: Paula Keene Pierce ; Maria Cruz 
; Morken, Timothy 
Cc: Histonet 
Subject: Re: [Histonet] Antibody diluent

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s 
really no such thing as a universal diluent like the ones offered by Leica and 
Agilent.  We get ours from Biocare, they offer five different diluents with 
different pHs and chemical makeups and we use three of these. I checked with my 
Biocare rep and iit seems that the one that’s most similar to Leicas is called 
Monet Blue.  I suggest you contact your Bocare rep and see if they can send you 
a sample of one or more them. Good  luck,Jeanine


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On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
 wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb

    On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:

 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet 
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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Re: [Histonet] Antibody diluent

[Jeanine Ronkowski via Histonet]

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s 
really no such thing as a universal diluent like the ones offered by Leica and 
Agilent.  We get ours from Biocare, they offer five different diluents with 
different pHs and chemical makeups and we use three of these. I checked with my 
Biocare rep and iit seems that the one that’s most similar to Leicas is called 
Monet Blue.  I suggest you contact your Bocare rep and see if they can send you 
a sample of one or more them. Good  luck,Jeanine


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On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
 wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:  
 
 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet  
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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[Histonet] QIHC Exam Preparers

[Jeanine Ronkowski via Histonet]

Hi Histo-peeps:I’m just wondering if someone can shed some light on who 
prepares the QIHC exam.  Everyone I’ve talked to about the test says that all 
of the questions are tricky and cover a lot older topics rather than more 
relavent information.  So how can we get some folks who understand today’s IHC 
world involved in this process?  TIA for your input
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Re: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D

[Jeanine Ronkowski via Histonet]

Katherine:Our lab did the same thing a few years ago and I received operating 
instructions for this system from a sales rep with Biocare that I know.  I'll 
send this document to you offline.  I can also put you in touch with the guy 
from Biocare.  As Colleen said, Biocare doesn't sell or support this instrumemt 
any more, but you can still get reagents from them.  I know of only three 
companies that can provide service and PMs.  Let me know if you need that info 
too.Good Luck,Jeanine
(jeanine.ronkow...@yahoo.com)
* * * * * * * * * * * * * * *
Hello Histonetters,

My company has acquired a refurbished ThermoFisher Lab Vision autostainer 3602D 
and I have questions on how to operate.  It is no longer supported by 
ThermoFisher so I am unable to get tech support through them.  Is there anyone 
who is familiar and available/willing to answer a few questions?

Thank you in advance.

Kate

Katherine Bummer
Nektar Therapeutics
455 Mission Bay Boulevard South
San Francisco, CA  94158
k.bum...@nektar.com
415.482.5716
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[Histonet] Histonet - IHC Manual staining

[Jeanine Ronkowski via Histonet]

Hi Dave:Our lab has used reagents from Biocare Medical for a very long time. 
They’re packaged in 6ml and 25 ml vials and ideal for hand staining. And their 
technical support is great tooJeanine

Hello Histoneters -

Does anyone still do manual IHC's? If so, which IHC staining kits would you 
recommend? Thanks.

Yours,
Dave

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[Histonet] IHC System from StatLab

[Jeanine Ronkowski via Histonet]

I’m pleased to report that I was finally contacted by a representative from 
StatLab that provided me with the information I was seeking.  I realize that 
their system may not be fully released, but I didn’t think it would be that 
difficult for them to provide information.  Turns out that this instrument is 
very similar to another manufacturer’s instrument that’s been on the market for 
a while.J


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Re: [Histonet] IHC System from StatLab

[Jeanine Ronkowski via Histonet]

All:For those of you who saw my posting last week and we’re wondering about the 
outcome, I’m sorry to say that I’ve received very little useful feedback.  Two 
individuals from StatLab sent me separate, cryptic messages indicating that 
they would be be happy to either meet with me or provide a demo of this unnamed 
instrument, but neither have provided me with a brochure or any other 
documentation, as I requested in my response.
I’ve never done this before, so I would like to ask this group – Is this 
behavior normal?  I’m gathering information on available instruments, and since 
I assume these folks would like to sell their new system, so why is it that no 
one will provide me with some basic information, like slide capacity, 
capabilities, reagent costs, etc?
Any and all feedback is appreciated.
Jeanine

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[Histonet] IHC System from StatLab

[Jeanine Ronkowski via Histonet]

I’ve heard from a number of people that Stat Lab plans to release a new 
instrument for immunostaining and I was wondering if someone, including the 
folks at StatLab, might be able to provide more information.Thx,Jeanine


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