Re: [Histonet] IHC and Histogel

[John Shelley via Histonet]

I agree with Dusko,

I have used histogel the same way and have also used it for FISH and ISH 
without any issues.

Kind Regards!
 
John J Shelley
Histology Core Manager
Sanford Burnham Prebys Medical Discovery Institute at Lake Nona
6400 Sanger Road    
Orlando, FL 32827    
Tel: (407) 745-2000 Ext.2517
Lab: (407) 745-2119
Fax: (407) 745-2001
email:  jshel...@sbpdiscovery.org   Please note email change


-Original Message-
From: dusko trajkovic via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, May 10, 2018 10:20 AM
To: Paula; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC and Histogel

We have used Histogel for years on cell pellets, and have not come across any 
IHC issues.Dusko 

On Thursday, May 10, 2018 7:13 AM, Paula via Histonet 
 wrote:
 

 Hello good morning everyone,

Can someone reply who knows if Histogel causes any interference with IHC
staining?

Thank you in advance,

Paula

Bio-Path Med Group

Fountain Valley, CA

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[Histonet] Florida Society for Histotechnology

[John Shelley via Histonet]

Hi All,

If you are interested in getting some CEU's, we have a great line-up of 
workshops. Click on link below for program details:

http://www.fshgroup.org/wp-content/uploads/2018/03/FSH_Program_May_17_20_2018-rev.pdf

The meeting will be taking place at the Renaissance Tampa International Plaza 
Hotel on May 18- 20, 2018.

If you have any questions contact John Shelley at jshel...@fshgroup.org. Look 
forward to seeing you at the meeting!

Kind Regards!

John J Shelley
2014-2018 FSH President

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[Histonet] 2018 FSH Annual Meeting

[John Shelley via Histonet]

Hello Histonetters!

The FSH Annual Meeting will be in Tampa, FL on May 17-20, 2018 at the 
Renaissance Tampa International Plaza Hotel.

We are looking for ideas for classes that you will be interested in attending.

We are also looking for speakers. Send abstracts to email below.

We want to hear from you. Contact us at fshgrouppresidentgmail.com

See you in Tampa.

Kind Regards!

John J Shelley
fshgrouppresid...@gmail.com

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[Histonet] alpha-glycerophosphate dehydrogenase staining protocol

[John Shelley via Histonet]

Hi All,

I have a group that wants to do an enzyme stain called alpha-glycerophosphate 
dehydrogenase on mouse skeletal muscle and was wondering if anyone has a 
working protocol. Thanks!!!

Kind Regards!

John J Shelley

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[Histonet] Frog tissue

[John Shelley via Histonet]

Good Morning Histonetters,

My daughter's class dissected frogs and her teacher gave me a frog to dissect 
and make histological slides and blocks for her. I have taken a bunch of tissue 
and not too sure how well preserved the tissue may be but I thought I would 
give it a try. I am sure that someone out on the Histonet has encountered such 
tissue and would like some help with what a good processing schedule would be. 
I tried to cut everything between 3-4 mm but may have some a slight bit 
thicker. I have all internal organs like heart, lung, kidney, stomach, 
intestines, female reproductive system and some eggs. I have outer skin, an eye 
and both front and back hands and feet for a better term, along with tongue and 
upper jaw of mouth. I have decaled some bone. So as you can see I have an array 
of tissue types that may or may not require different schedules on the tissue 
processor and any help would be greatly appreciated.

I thank you in advance!

Kind Regards!

John J Shelley

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[Histonet] Plant tissue

[John Shelley via Histonet]

Happy Friday!

I have given a request for service to me to do some plant tissue histology and 
I must admit this is a new area for me. I plan to do both paraffin and frozen 
sectioning and would like to ask if there may be anyone has a protocol on how 
to handle such tissue in regards to a tissue processing schedule and also steps 
to take for freezing. Any information would be greatly appreciated.

Have a great weekend!

Kind Regards!

John J Shelley
Histology Core Manager
Sanford Burnham Prebys Medical Discovery Institute at Lake Nona

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[Histonet] Florida Society for Histotechnology Spring Meeting

[John Shelley via Histonet]

Hi All,

Happy Monday!

I would like to inform all histonetters both in Florida and abroad that our 
meeting program has been finalized and registration is now open. We have a 
wonderful array of workshops that will appeal to all levels of experience. We 
have our 3rd annual All-Day Supervisor and Administrator Forum on Friday, May 
20th, 2016 along with our All-Day HT Preparedness Workshop for students and 
those who are looking for a fresher on Saturday, May 21st, 2016. Along with 
general histology, safety, IHC, molecular, digital imaging and much more. 
Please use links below to look at our program and then our links for 
registration to the meeting and hotel.

FSH Online Registration

FSH Online 
Program

FSH Hotel 
Registration

Look forward to seeing you all in sunny and warm St. Pete., Florida at the 
Bayfront Hilton on May 19-22, 2016.

Kind Regards!

John J Shelley
2014-2016 FSH President

Histology Core Manager
Sanford Burnham Prebys Medical Discovery Institute at Lake Nona

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[Histonet] Tissue Arrays

[John Shelley via Histonet]

Good Morning,

I have purchased some arrays from a company in the past and had some very 
varying results. I prefer not to mention the company and so my request is if 
those of you who do not have the luxury of having tissue at your disposal to 
make your own tissue arrays from whom are you buying them from. Once purchased 
have you used them on IHC platforms like Ventana Benchmark/Ultra or Leica 
Bond3? Were your results consistent across the slide?

I would like to add that I am looking for neuroblastoma tumor arrays. Any help 
will be greatly appreciated. Thanks in advance!

Kind Regards!

John J Shelley

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[Histonet] 2016 FSH Annual Meeting Abstract Request

[John Shelley via Histonet]

Good Morning,

I know that there are many people out there that have a passion to teach what 
they know and we would very much like to put a call out to those who would be 
interested in presenting a workshop or two at our 2016 Florida Society for 
Histotechnology Annual meeting in the beautiful gulf coast area of St. 
Petersburg FL on May19-22, 2016 at the St. Pete Bayfront Hilton. We will have 
two and half days' worth of lecture time, starting with our popular 
Administrator and Supervisor Forum, the whole day HT preparedness workshop for 
students or those who are planning on taking their HT/HTL certification through 
ASCP. Please consider this great venue and submit your abstracts.

We are interested in topics on special stains, IHC, In Situ, Lean, billing, 
safety, digital pathology, ergonomics, cytology, CAP/CLIA inspection tips, 
leadership and any other topic that is relevant to today's histology 
laboratory. Please send abstract to John Shelley at 
fshgrouppresid...@gmail.com. I look forward 
to your submissions. Vendor submissions welcomed!

Our plan will be to notify you by late December to early January so please keep 
your calendars open until you hear back from us.

Kind Regards!

John J Shelley
2014 - 2016 FSH President

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