Re: [Histonet] Premaking slides
This does apply to Frozen and Touch preps. Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you On Thu, Apr 25, 2024 at 1:20 PM Jay Lundgren wrote: > *USE CAUTION - EXTERNAL EMAIL* > -- > You mean for frozens? > > On Thu, Apr 25, 2024 at 12:44 PM Karen Schieberl CA-San Francisco via > Histonet wrote: > >> Good Morning, >> I am not sure if there is a CAP or Joint commission Policy on pre making >> slides for intraoperative consultation. Can someone let me know if there >> is one? I have looked but was unable to find one. It just seems like >> errors can be made especially if you have multiple patients or for some >> reason the information on the slide does not match the patient. Any help >> would be greatly appreciated. >> >> Thanks, >> Karen Schieberl HT ASCP >> >> Lead Histology Technician >> >> St. Mary's Medical Center >> >> 450 Stanyan St. >> >> San Francisco, Ca. 94117 >> >> 415-750-5751 >> >> karen.schieberl@ commonspirit.org >> >> >> >> >> Caution: This email message, including all content and attachments, is >> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The >> information contained in this email message is intended only for the use >> of >> the recipient(s) named above. If the reader of this message is not the >> intended recipient or an agent responsible for delivering it to the >> intended recipient, you have received this document in error. Any further >> review, dissemination, distribution, or copying of this message is >> strictly >> prohibited. If you have received this communication in error, please >> notify us immediately by reply email. Thank you >> >> Caution: This email is both proprietary and confidential, and not >> intended for transmission to (or receipt by) any unauthorized person(s). If >> you believe that you have received this email in error, do not read any >> attachments. Instead, kindly reply to the sender stating that you have >> received the message in error. Then destroy it and any attachments. Thank >> you. >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> <https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwMFaQ=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c=fnkbvT8D25g0nM9qsmusaIIGXMRtGYAvOTiZHWzeQ6Lb9v86eSROCr3zok0ftoBM=eA5A20TalbYgTy_IUtHpfZKSkMDgrxidp1yLTLOdzc_eUqtXw-WEoDUNIDfp67bn=m-Ly3i0Wyosg9X_182mk74S2YBlEVEHVH-NM2S-O5oY=> >> > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Premaking slides
Good Morning, I am not sure if there is a CAP or Joint commission Policy on pre making slides for intraoperative consultation. Can someone let me know if there is one? I have looked but was unable to find one. It just seems like errors can be made especially if you have multiple patients or for some reason the information on the slide does not match the patient. Any help would be greatly appreciated. Thanks, Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MMR
Good Morning, I am looking for literature on minimum and maximum formalin time for breast tissue for MMR testing. I know about this time for ER/PR, HER2 and fish testing. I need the literature for the MMR fixation time on Breast tissue. I thought that you do not even do MMR on breast tissue. Your help is greatly appreciated. Thanks, Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC gunk
Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system. I have put fresh reagents on the instrument. This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue. I do not see this stuff elsewhere on the slide, only the tissue. Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue. I use DiH20 from the hospital system. Not sure if this may be the problem. I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab.
I got mine through Thermo Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you On Wed, Mar 1, 2023 at 3:04 PM Jay Lundgren via Histonet < histonet@lists.utsouthwestern.edu> wrote: > USE CAUTION - EXTERNAL EMAIL > > .. > Who sells that block deparaffinizer doohickey that everyone has in their > lab. What brand is it? Leica? I don't have one in front of me right now. > > Thanks, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > Histotechs are the foot soldiers in the battle against Cancer. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CqLityr3mSQ!GHohhm5t61fx5WIqk10A1j1agEowfqUHV4ta3OZ5US9GoWlK5ZYYYzLLhsql0_vxxCDE4T0OtBxGKbplHxK4mah8O7khxSPV-w$ > Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thin Prep
Does anyone have the contact information for a Thin Prep rep in the US. I need to get in touch with them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hiring per diem in SF
Good Morning, I have an opening for a per diem position. Right now it is for at least 8 hours per week but can develop into more hours. Apply Dignity Health St. Mary's Medical Center St. Mary's Medical Center San Francisco. If you have any questions email me. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology
Good Morning, I rarely do Special stains or IHC's on Cytology cytospins or thin prep type slides.Should these slides be charged, air dried or fixed in 95% alcohol before doing Special stains or IHC's on them? Is there a good book or some sort of publication on cytology procedures regarding the above mentioned. I have tried and look it up and it seems all over the place on what to do. Any help would be greatly appreciated, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Printmate/Slidemate
Good Morning, I was wondering if anyone has a procedure written up on how to train someone for the Thermo Printmate AS and Slidemate AS that I could get? This would be for my Policy and Procedure manual. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RVU for Histology
Good Morning, I think we are getting the short end of the stick when it is coming to productivity. Can anyone give me insight on how they are calculating their RVU's for productivity in the Histology Lab? I am busy as all get out and administration is saying our productivity is low. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] San Francisco Per diem Postion
Good Morning, I am still looking for a Histology Tech. for a per diem position here at St. Mary's Medical Center. You will at times be working by yourself so strong trouble shooting skills are a plus. I do Routine Histology, IHC's and manual special stains. This position will cover me for Admin days, sick and vacation time. I can offer a minimum of 8 hours a week at this time but a good chance of more hours coming shortly. We are bringing in more work so the workload will be more. I am working to try and get this into permanent part time position. Please apply on Dignity Health website. St. Mary's Medical Center, San Francisco, Ca. I look forward to hearing from you. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathnet
Good Morning, Does anyone know how to extract the cost per slides out of the Pathnet program? I am looking for my H, IHC's and special stains. I just need the technical charge. Thanks in advance, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT per diem position in San Francisco
Good Morning, We are actively looking for a per diem Histology Technician to cover for vacations and administration days. Will more than likely need a person 8 hours a week for now. Must be able to work independently without supervision. We hand do special stains. IHC experience is preferred. If you have any questions please do not hesitate to call me Karen Heckford at 415-668-1000 extension 6167. This position could possibly develop into a part time position later. The hours are 430am to 1pm but can discuss working around your schedule if needed. Please go to Dignity Health St. Mary's Medical Center San Francisco to apply. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hiring per diem in San Francisco
Hi, We are looking for a per diem Histology Technician or HTL with IHC experience. You will be covering the only Histology Tech for Vacations, admin days and sick time. You must be able to work by yourself. Strong troubleshooting skills a plus. We hand stain special stains. The hours are 0430-1230pm Monday through Friday but we may be able to shift some of the hours around if you are working another job. This job could possibly turn into a part time gig down the road. Please go to Dignity Health Online to apply: We are St. Mary's Medical Center in San Francisco. You can call me and leave a message at the number below if you have any questions or respond back to my email address. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org<mailto:karen.heckf...@dignityhealth.org> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT per diem and Pathologist
I am looking for a per diem Histology Tech to cover me for Vacations and Administration Days. Must know IHC's and manually do special stains. You will be working independently since I am the only one here that does this job. The hours are 4:30am to 12:30pm Monday-Friday. I can work around the schedule a little to accommodate if someone is working another job. If interested please go the Dignityhealth website and location will be St. Mary's Medical Center San Francisco to apply. We are looking to fill this position ASAP. We are also looking for a Pathologist to work with our Pathologist here on site. We do some Non-gyn but mostly surgical. If you know of a Pathologist that is interested have them email me and I will get them in touch with our Pathologist. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Surgical Hardware
Good Morning, We have an abundance of surgical hardware we need to get rid of from our Pathology Department. How do you get rid of your surgical hardware? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen section room
Good Morning, I have a question about how much reagents you can keep in a frozen section room. Our frozen section is a little room off of Surgery. When it was designed now one thought to ask us what it was going to need so there is little ventilation down there besides the typical room output and input. No hood!! We have a small H stain line, DQ and Toluidine Blue. Can I keep a small amount of each reagent down there for topping off and changing out reagents? I was thinking Pint bottles. I am pretty sure there is regulations on this! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER/PR question
Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TMA slides
Good Morning, Does anyone know where I can purchase some CMV TMA slides? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Assistant
Good Afternoon, We are looking for a per diem person for our Pathology and cytology department. This person will be picking up specimens, doing accessioning, making cassettes, maintaining the grossing room, tissue dumping, assist with FNA's, prep cell buttons and do cytospins and staining. Also go to surgery and do specimen pick up and maintain the Frozen section room. I am guessing this person would be just a lab assistant dedicated to Pathology? Not sure what the correct Job title should be. This person would cover for vacations, sick time etc.Also this person may get to dabble in some Histology stuff as well under the supervision of the Tech if they want to get more hours. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide printer and cassette label
Good Morning, I am looking for suggestions for a slide printer and cassette labeler for a smaller lab. Any suggestions. I need it with the two patient identifier. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unsubscribe
Hi I would like to unsubscribe. Kind regards Karen Inge Nielsen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quick release clamp
Good Morning, Does anyone know where to get the Quick release clamp repair kits for the microtome? The one that has the springs in it. I remember a long time ago getting them but I cannot remember from where. Do they still sell them? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Non gyn cytospins
Good Morning, Is there a difference between the time screening Non-gyn cytospins vs. Gyn? If so where can I find the publication to back this up? Just wondering. We got inspected and this came up because our Pathologists screen and read out the Non gyn cytospins. We do not do Gyn's here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathnet users
Good Morning, We have Cerner Pathnet here and I need to pull three month's worth of IHC codes. 88341, 88342 and 88344 for billing purposes. We need to know how many IHC's we charged for the last three months and what they were. Does anyone know how to go about pulling this information out of this system? Thank you, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prisma stainer
Good Morning, I am not familiar with Sakura Prisma Autostainer. I heard the stainer can come with a tape coverslipper. Is the stainer, link and coverslipper all one unit or is it individual. I maybe getting a stainer that has the link does that mean that is the coverslipper or just the link between the stainer and coverslipper? How wide is the stainer with the coverslipper. I looked up the information but wanted some real user input. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HP antibody
We have used Biocare’s Hp concentrate and predilute for years. Very consistent and the concentrate is strong. There is no background and it is very clean. Karen Lahti, HT, QIHC Lab Manager Arizona Digestive Health Phoenix, AZ > On Apr 25, 2017, at 5:37 AM, Pairan, Kelly via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hello Histoworld, > Happy Lab Week! We have not been able to get H. pylori antibody from our > current supplier for a while. Who is the supplier for your lab and do you > have any stock issue? > > Thanks and have a great day! > Kelly Pairan, HT (ASCP) QIHC(ASCP) > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Tek prisma stainer
Good Morning and Happy Holidays, I was just wondering people that have the Prisma, does the monitor on the top right corner swivel and can be positioned in different positions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PathNet HELP UPDATED
Good Morning, I need to contact some of you. If you have Histology in-house, use the PathNet program and do CAP. I have some questions on how you handle Inventory Management and a couple of other things. If it is okay to give you a ring please let me know. Thank you so much, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PathNet HELP
Good Morning, I need to contact some of you. If you have Histology in-house and use the PathNet program. I have some questions on how you handle Inventory Management and a couple of other things. If it is okay to give you a ring please let me know. Thank you so much, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Melanin Bleach
Good Morning, I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking.I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clinical Lab vs. Antomical Pathology
Good Morning, I am curious to know how many Pathology Departments get lumped into Clinical Lab? Do you wear the same working attire? Our Pathology Department is really small. We are required to go over the red lines in surgery to get to our Frozen Section Room numerous times a day. They want to put us in the same Scrubs as the Clinical Lab which would not allow us to go over the red line. The color of scrubs designates where you are from in the hospital. I for one do not know how to draw blood or even know what color top tube to use the CLS and phlebotomist do. I only know only about Bone Marrows. I am getting frustrated because to me Anatomical Pathology and Clinical Lab is two different species but they keep trying to treat us the same. The only thing we share is the Lab Director and the Pathologists with Clinical Lab. Feeling like I am not being heard. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat with UV disinfection
Our Mohs Lab is CAP accredited. Every seven days of use, cryostats are shut down. Once machine has been cleaned and dried we use Cavi-wipes(super Sani-Cloth from PDI Inc.) We then document disinfection date on QC Log. We have three cryostats, we rotate a cryostat a week for disinfecting purposes. We added this to our Equipment: Cryostats/Microtomes policy. Hope this helps. Karen Pfaff, HT (ASCP), BA Lead Histotechnician Skin Cancer Center Froedtert Health Phone: 414-805-6010 Fax: 414-805-5325 -Original Message- From: Terri Braud [mailto:tbr...@holyredeemer.com] Sent: Friday, March 11, 2016 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Cryostat with UV disinfection For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfectant that will work at cryostat temps? Curious minds would like to know! ANP.23410 Cryostat DecontaminationPhase II There is a written procedure for the decontamination of the cryostat at defined intervals, and under defined circumstances, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Terri L. Braud, HT(ASCP) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] temperature charts
Hi! We have a temperature chart form that we created and use across all instruments and areas. It is one chart for the whole year and has an area for review date and signature at the end of each month. We have daily thermometers. These may be regular thermometers or built in to the instrument. Twice a year we check those daily thermometers against our "gold standard" calibration thermometer. The one we use is a Fisher "Ertco" Standard. Min/Max daily thermometers are also cross checked even if they come with their own calibration report. If the daily is off by +/- 1 deg. C then we replace the daily and monitor it to be sure it is not the instrument that is faulty. We collect all temperature QC Charts and Calibration reports at the end of each year for JCAHO inspection. Hope that helps! Thank you, Karen Karen P. Bryan, HT (ASCP)cm Sr. Neurohistology Specialist Department of Pathology/Amory 3 Brigham & Women's Hospital 75 Francis Street/Boston 617-732-7534 (Internal extension 2-7534) Karen The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Expiration dates of ammonium sulphide
Dear Mr. Morken, I found your thread on Histonet regarding ammonium sulfide for muscles. We are running a panel including ATPase every week and have been having problems with the manufacturer with the supply. As of today we were told by FISHER that they are back ordered indefinitely. What company are you using to buy the 25 ml bottle? I also see you stated back in 2010 that the concentration doesn't matter, but just so you have the information we are using 21% aqueous ammonium sulfide light (as stated on the bottle). I hope you are still at this email address! Thank you in advance for any help you can provide. Karen Karen P. Bryan, HT (ASCP)cm Sr. Neurohistology Specialist Department of Pathology/Amory 3 Brigham & Women's Hospital 75 Francis Street/Boston 617-732-7534 (Internal extension 2-7534) The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mohs and what is considered grossing?
In my lab I am checking my CAP checklist. I just took over the Supervisors job after she retired. In looking at our checklist it mentions that Mohs testing is a High complexity test which requires qualifications. Part of the checklist states “Grossing specimens -nonpathologist.” In research to see if Mohs actually considers Grossing as part of there protocol. Surgeon places tissue on a piece of guaze with proper orientation. Designates where hatches or scores are located (12 o’clock, 3 o’clock,etc..) Hands it over to tech. The tech then draws map of specimen, bisects it usually across the scores, depending on size of specimen could be more pieced if larger. The tech then inks specimen, relaxes tissue so specimen will lay down properly. Gives an accession number to it then embed tissue in OCT and continues to cut tissue and place on slide. The slide is then stained, coverslipped and handed over to Mohs surgeon. Just wondering if this part of manipulating the tissue is considered grossing. Thank you for any information you may have. Karen Sent from Windows Mail Sent from Windows Mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help: IHC service
HELP: Hi, Is there anybody can provide me the price list/structure of the custom IHC services? For example, to test 200 tissue slides and get 200 images, how much does it cost? Thank you very much in advance, Have a nice weekend, Best Regards, Karen k...@prosci-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temperature logging
Good Morning, I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this. 1st Question: What is your temperature range? 2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart? The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temperature for slides
Okay I just got a weird request for monitoring the room temperature where I have my slides for that I use for Histology. We are talking blank slides. I have never heard of this before. Who else does this??? Apparently JCAHO wants this. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin sectioning possibly calcified heart valves
Have you tried using RDO on the block surface? On Aug 12, 2015 12:30 PM, Jennifer Connell via Histonet histonet@lists.utsouthwestern.edu wrote: Hello, I am having trouble with sectioning of paraffin embedded human aortic valve samples. I received the embedded samples from a collaborator who had the embedding done by the pathology department at her institution. Six of the 12 samples sectioned fine but the remaining samples are developing ribbon cuts and/or are crumbling in areas when sectioned. The suggestion that calcification is causing the issue has been raised by other members of my lab. Some of the samples do appear to have calcific nodules while others may have a small bit of calcification along the valve. The samples with what appears to be calcific nodules do feel stiff at the nodules and are not being cut flat with the rest of the sample there. If I reposition the samples to a new part of the blade, the defects (ribbons or crumbling) are still in the same place or reappear in the same place quickly. I use Tissue-Tek Accu-Edge disposable high-profile microtome blades on a Lieca RM 2135 microtome. For the samples that do not have the obvious nodules - is it possible this was an embedding error and I may be able to re-embed the samples and proceed? If calcification is the issue - is it possible to de-calcify the valves after they have already been fix, dehydrated, and embedded? Or are there any other suggestions on how I could proceed? Any advice or suggestions would be greatly appreciated. Thanks. Jen -- Jennifer Petsche Connell, Ph.D. Lab Manager and Research Scientist Integrative Matrix Mechanics Laboratory http://www.proteoglycan.com/ Department of Bioengineering Rice University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Handline paraffin
Is there any written documentation? On Aug 3, 2015 11:10 AM, Rene J Buesa via Histonet histonet@lists.utsouthwestern.edu wrote: It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or irritating substance. It is just an oil of high molecular weight (mineral oil)René On Monday, August 3, 2015 12:48 PM, Johnson, Carole via Histonet histonet@lists.utsouthwestern.edu wrote: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana H.Pylori antibody
Hi Julia, We have a GI lab and use Biocare's H. Pylori concentrate on Biocare's IntelliPath. We run about 50-75 H. pylori IHC each day. They are very clean and specific. Our Pathologists and myself are extremely happy with Biocare customer service and products. On Thursday, July 30, 2015, Cates, Julia via Histonet histonet@lists.utsouthwestern.edu javascript:_e(%7B%7D,'cvml','histonet@lists.utsouthwestern.edu'); wrote: Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to clean up the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253- ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Thank You, Karen Lahti, HT ( ASCP), QIHC 480-236-6559 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breast fixation
Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum.Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Our Histology laboratory currently uses Mercurochrome to mark specimens, but we need to discontinue using it. Can anybody tell me of a good substitute. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin waste
I was just wondering the Labs in the San Francisco Bay Area. How are you getting rid of your formalin waste? Are you Neutralizing your formalin like with Neutralex or having it picked up and taken off site? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ATPase 9.4 Troubleshooting
Dear Friends, I am having trouble with the ATPase 9.4 stain on muscle biopsies performed on frozen sections. The sections look extremely dark and almost have a digested look to them. To troubleshoot we: * made all new solutions, * opened a new container of ATPase (stored in the -15 deg. C. freezer) * the oven has been tested and confirmed to be stable at 40 deg. C. * the pH is stable at 9.4 (+/- 0.02) * only distilled water is used for all solutions where required and for rinses between steps. The ATPase 4.3 has some artifact but definitely is readable and not like the 9.4. All of the other sections for the other stains in the protocol (HE, Trichrome, NADH, ORO, PAS) are coming out great and the sections do not show any freezing or microtomy artifacts. Has anyone else run into this problem? We have run the same muscle cases 3 times in the last 2 days after making the changes to all of the solutions to prepare the ATPase. What are we missing? I may be able to upload a photo if you need to see the issue clearly. Thank you so much for your help! Karen Karen P. Bryan, HT (ASCP)cm Sr. Histology Specialist Department of Pathology/Amory 3 Brigham Women's Hospital 75 Francis Street/Boston 617-732-5454 (Internal extension 2-5454) The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC apoptosis marker (caspase 3 or similar)
One of our pathologists wants to know if anyone is running IHC apoptosis markers (caspase 3 or similar) on cattle. Thanks, Karen Sverlow CAHFS, UC Davis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cap ER/PR
Good Morning, Apparently while I was out on disability a CAP ER/PR PM2-A 2014 did not get done. Somehow one of our pathologists got it and forgot she had it. That being said, does anyone have a equivalent test they do instead. We only do this CAP testing to show competency. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
You could send them to Beaumont Health System. Sent from my iPhone On Sep 10, 2014, at 1:31 PM, WILLIAM DESALVO wdesalvo@outlook.com wrote: University of Michigan MLabs William DeSalvo, BS HTL(ASCP) From: ttro...@petersonlab.com To: histonet@lists.utsouthwestern.edu Date: Wed, 10 Sep 2014 12:22:55 -0500 Subject: [Histonet] (no subject) I was wondering what facility labs are sending nerve and muscle biopsies to. Thanks, Travis Troyer Peterson Laboratory Services Manhattan, KS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathnet program
Hi, Any of you using the Pathnet program. We just went live and now they are telling me that our labels will not work with the program. We are currently use 7/8x7/8 square labels 4 across Stainershield labels. I don't understand why they will not work? We did use the Powerpath 9.2 program before. Can't we just go into properties and change it to work? Any input would be greatly appreciated. Thanks, Karen Heckford HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125, Issue 24
Good ;Morning, We have the Leica 1950 in our Laboratory and are extremely pleased with the insturment. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital - South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen@albertahealthservices.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: April 21, 2014 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 125, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Xylene substitute for processing GI biopsies (Joe W. Walker, Jr.) 2. Alport's testing (Houston, Ronald) 3. Re: Xylene substitute for processing GI biopsies (Rene J Buesa) 4. Recommendations cryostat (Pathology-Histology Sr. Supervisor) 5. RE: Paraffin (Cameron, Elizabeth) 6. COM 30450 (Piche, Jessica) 7. RE: Recommendations cryostat (WILLIAM DESALVO) 8. RE: Recommendations cryostat (Bea DeBrosse-Serra) 9. anti mouse iNOS (Anna Hughes) 10. Re: RE: Recommendations cryostat (Kim Tournear) -- Message: 1 Date: Sun, 20 Apr 2014 23:36:05 + From: Joe W. Walker, Jr. joewal...@rrmc.org Subject: RE: [Histonet] Xylene substitute for processing GI biopsies To: Rene J Buesa rjbu...@yahoo.com, Lori Gemeinhardt loreli...@mac.com,histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 3c2378778400ad448ada6fd6bdb7182a0...@rrmbx03.rrmc.local Content-Type: text/plain; charset=iso-8859-1 Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.orgwww.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt loreli...@mac.com wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from
SV: [Histonet] Xylene substitute for processing GI biopsies
Hi Since you use a tissue tec VIP from Sakura you could use tissue clear from Sakura. I have been a histotech for 11 years an have only used xylene substitute for processin. The past 3 years on Peloris from Leica and before that on VIP E300. I use Tissue Clear from Sakura and it works as well as xylene. You just need to entlong the clearing steps a little. I use also Tissue Clear in the stainingprocess. Just go for it I work xylenefree with results as good as xyleneprotocols. Kind regards Karen Fra: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] p#229; vegne af Lori Gemeinhardt [loreli...@mac.com] Sendt: 18. april 2014 02:18 Til: histonet@lists.utsouthwestern.edu Emne: [Histonet] Xylene substitute for processing GI biopsies Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathnet
Does anyone in the local San Francisco Bay Area use the Cerner Pathnet program. I need some help!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: proceesing of GI Bxs
We have a medium GI lab using the old, old VIP's. We have been very successful with two processing schedules, long and short. We found that we needed to add a bit of heat to the last xylene so when the tissue hit the first paraffin, they would start to infiltrate. The cold cassettes solidified the paraffin and the time spent was melting the paraffin not infiltrating the biopsy. For the longer run, we add 10%NBF and a bit longer in each solution. Short: 1. 70% reagent alcohol - 15 min, 40C, P/V 2. 85% reagent alcohol - 10 min, 40C, P/V 3. 85% reagent alcohol - 15 min, 40C, P/V 4. 100% reagent alcohol - 10 min, 40C, P/V 5. 100% reagent alcohol - 10 min, 40C, P/V 6. 100% reagent alcohol - 10 min, 40C, P/V 7. Xylene - 20 min, 40C, P/V 8. Xylene - 20 min, 50C, P/V 9. Paraffin - 10 min, 58C, P/V 10. Paraffin - 30 min, 58C, P/V 11. Paraffin - 45 min, 58C, P/V Karen Lahti, HT (ASCP), QIHC Arizona Digestive Health 602-687-7468 On Apr 3, 2014, at 9:33 AM, Arguello, Daniel arguel...@ihn.org wrote: I'd appreciate a copy of that processing schedule for GI bxs as well. Thank you in advance Sincerely, Daniel Argüello, BS, CT (ASCP)CM Anatomic Pathology Services Coordinator Inspira Medical Center Woodbury 509 North Broad Street Woodbury, NJ 08096 856-853-2030 Ext 2808 856-853-2183 Fax arguel...@ihn.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vicki McKaughan Sent: Thursday, April 03, 2014 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] proceesing of GI Bxs Could someone please send me your processing schedule for GI bxs. We are using a Sakura VIP5 and are having problems with over drying and occasionally with infiltration of paraffin. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathology Protocols
Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pathology Protocols
Hi Douglas, Yes... For the most part, they are on board with this... So long we can get the information that they want/need on the screen/monitor(s). We just need to figure out how to make that happen. Figured I'd ask out in Histoland to see if we can learn from someone who has already made/created that process. Thanks for the reply! :) Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: Douglas Porter [mailto:doug.por...@caplab.org] Sent: Tuesday, March 11, 2014 10:55 AM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathology Protocols The key to your plan is having pathologists that are willing and/or able to use the computer to do something normally done by someone else. Once you cross this barrier, the rest is logistics. Good Luck!! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4335 / Virus Database: 3722/7177 - Release Date: 03/10/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Fungus Controls
Thanks to everyone who replied to my fungus control request. I had a lot of great responses!! :) Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Thursday, February 20, 2014 2:57 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fungus Controls Importance: Low Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fungus Controls
Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 123, Issue 15
Yves, You did not mention the melting point of your paraffin...any temperature above 60 degrees or prolonged exposure of heat may destroy protein in the tissue causing structurally damaged antigenic sites. This would invalidate your IHC results as staining would not be representative of what was originally present. Leigh Sent from k. Leigh's iPad. On Feb 15, 2014, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: TJC on IHC Neg Controls (Terri Braud) 2. HISTOPALOOZA April 25 - 27, 2014 (Zimmerman, Billie) 3. In need of Amyloid Control Tissue (Nani Brabson) 4. End time for fixation of breast tissue (Glenn Hauck) 5. RE: End time for fixation of breast tissue (Morken, Timothy) 6. ANP. 23045 performance of all instuments (anita) 7. adverse effect of prolonged paraffin embedding onstaining ? (Yves Heremans) -- Message: 1 Date: Fri, 14 Feb 2014 13:31:01 -0500 From: Terri Braud tbr...@holyredeemer.com Subject: [Histonet] RE: TJC on IHC Neg Controls To: histonet@lists.utsouthwestern.edu Cc: erin.mar...@ucsf.edu Message-ID: BFBF7C6B9627B947B74CBEBD45CE368B1246C1D3@hrex-svr.holyredeemer.local Content-Type: text/plain;charset=us-ascii I don't know who your manager talked to, but he/she has been misinformed. There is no such specific Joint Commission Standard. Here is what they state, copied from their standards: QCP.2.1.1 When immunohistochemistry is performed, the laboratory has appropriate quality control processes. Also, TJC defers to CAP regulations Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 4 Date: Thu, 13 Feb 2014 18:20:59 + From: Martin, Erin erin.mar...@ucsf.edu Subject: [Histonet] Negative Controls Hi All, We are a Joint Commission inspected lab but after CAP changed their requirements for negative controls we were hoping to drop them too. My manager contacted TJC and asked what their position was regarding negative controls. They responded that they have not changed their requirements and therefore still want to see negative controls. Has anyone by inspected recently by TJC and had this issue come up? Thank you! Erin Martin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. -- Message: 2 Date: Fri, 14 Feb 2014 18:31:59 + From: Zimmerman, Billie bzimm...@gru.edu Subject: [Histonet] HISTOPALOOZA April 25 - 27, 2014 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 7b3deb32e69c034eacb479059c5de3ff810...@ex-mlb-03.ad.georgiahealth.edu Content-Type: text/plain; charset=us-ascii I hope all of you wondered where I've been. I have survived the winter ice storm in Augusta, Georgia. Let's just say if Jim Cantore, from the Weather Channel, arrives in your city, you know it's the kiss of death! When I heard he had arrived, I knew we were doomed. But, I'm bck! Today's feature is a tag team, Jim and Theresa Burchette. Jim will be conducting a workshop titled Immunohistochemical Detection of Infectious Diseases. The brochure says audience participation is encouraged. I have no idea what that could mean. It's either sharing of proper staining patterns or how to hand tie a tootie fruitie fly for fishing. Theresa's presentation is titled Immunoreactivity in Normal Tissue. This is an extremely helpful workshop for selecting and maintaining IHC tissue controls. Both of these workshops will serve as great contact hours if you are wanting to maintain your QIHC or are interested in learning
[Histonet] RE: Ventana LEAN team
Hi Amber, As part of our Vantage implementation process, a workflow consultant initially came out to look at our processes. He was very nice and had a lot of great suggestions. He was never pushy and I had control of what I wanted to change or not change. He basically was there to see how the implementation would go according to what we wanted and how things were set up. Having his overall assessment of our workflow and how we could improve steps was very helpful with our final implementation of the Vantage system. Even if you are not getting the Vantage Specimen Tracking System, the workflow assessment will help you in your overall lab design. Use his/her suggestions as you want... It's your lab... We are all unique. :) Good luck, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Acccessioning area/Grossing area
We do it that way as well. It seems to flow quite nicely and it works well for us. Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 07, 2014 3:35 PM To: 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acccessioning area/Grossing area Yes. We have ours that way. Our whole lab flows around the room. This is the LEAN way. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acccessioning area/Grossing area Do you any of you have your accessioning area right next to your grossing station so that the person accessioning can put cases on a cart and the person grossing can just grab each case and gross it in as they are being accessioned? Just curious about this setup. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Purple/Blue haze on ISH
Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller. That's the only real difference from before and after. Protocols have not changed and they've checked everything else... buffers and such. Any ideas on why this would happen? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Tracking Systems
Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!:) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was Why would you want to do that? Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely unimaginative in their outlook. That is why Ventana, Leica and others were able to step into that gap and develop their own systems. And at places like U of W they developed their own tracking systems and fully integrated it to their LIS because their LIS did not have anything even planned for barcoding. But now these same LIS companies have finally figured out that that the barcoding ship is leaving the dock and they are scrambling to develop their own systems to compete with the stand-alone systems like Vantage and Cerebro. Due to that competition these LIS companies may not be willing to allow true bi-directional communication - it totally undercuts anything they may develop. They are already at least 5 years behind the curve and can't allow the other companies to gain any further advantage. Tim Morken -Original Message- From: Jesus Ellin [mailto:jel...@yumaregional.org] Sent: Friday, November 01, 2013 6:54 AM To: Morken, Timothy; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems I would like to chime in on this Bill and Tim both gave great examples of what it takes to move in this direction. But I would also like to understand why there are so many challenges in getting the data back into the APLIS with updates and orders. I would tell people the biggest concern that I have the data being produced by a system should be accessible across all gradients of the workflow and not just the unique tracking system. Not only is this tracking system positioned to help out histology with what is being explained below, but it is the foundation work in taking steps to get to the next level when talking Digital Pathology, molecular, Personalized medicine, Pathologist cockpit, and algorithm analysis. With the looming cuts
RE: [Histonet] Specimen Tracking Systems
Thanks for the reply Tim! Yes... I agree, and too much paper is wasted, since digital copies all available in the software that we utilize. We have no residents or PA.s in our lab, and the Histotechs probably do 80% of the daily gross. We also do 100% of the accessioning of all surgical specimens. We would be more than happy to get away from using the paper reqs, plus it would keep the paper copies out of the gross room and clean. There is the potential for paper slips to get contaminated when grossing specimens and then they are given to the transcriptionists. This process could be so much better. Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] Sent: Friday, November 01, 2013 11:23 AM To: Bauer, Karen L.; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Karen, we are going to move to a system as you describe. At accessioning all paperwork will be scanned and none will follow the specimen. The critical trick is getting residents and pathologists to look at screens rather than hard copies. We spend way too much time printing and collating hard copies of reports ever day. Tim Morken Pathology UC San Francisco Medical Center San Francisco, CA -Original Message- From: Bauer, Karen L. [mailto:bauer.ka...@mayo.edu] Sent: Friday, November 01, 2013 9:03 AM To: Morken, Timothy; 'Jesus Ellin'; 'WILLIAM DESALVO'; 'Matthew D. Roark'; 'histonet' Subject: RE: [Histonet] Specimen Tracking Systems Importance: Low Jesus, Tim, and Bill... Wonderful communication about specimen tracking, bi-directional capabilities, and creating a histology lab that will be effective in the years to come!! I loved reading your information! We also have the Vantage system and it's helped us decrease our slide labeling error rate to 0%! It's wonderful and I'm so glad we finally had it implemented last April. Our workflow seems much more efficient, since we no longer have to perform all of those manual reconciliation steps. Yes, the bi-directionality of the systems (we have Sunquest CoPath) is a downfall of the software, but the pros of Vantage outweighed that flaw. I'm hoping that in the years to come, that will be fixed. A new question for the Histo group... We are trying to get away from printed grossing working drafts that are submitted with the slides and delivered to the pathologists. We would like the docs to scan the slide at their microscope and have the patient information show up on their computer. The pathologists still want the paper requisition from the specimen, so I suggested to have the requisition scanned and attached in the digital format. This way, when the doc scans the slide, the CoPath working draft, the patient clinical hx, and the scanned req slip can be viewed. Is anyone doing this in their lab right now? If so, I would really like to hear more about how you and your LIS made that happen. Thanks so much and Happy Friday!:) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 01, 2013 10:06 AM To: 'Jesus Ellin'; 'WILLIAM DESALVO'; Matthew D. Roark; histonet Subject: RE: [Histonet] Specimen Tracking Systems Jesus, the topic of bi-directional interfaces is fraught with both technical and political issues. Technically it is certainly possible (As a software developer colleague of mine always told us - 'don't ask if I can do it, just tell me what you want. It's just software'). The issue is who pays for it, and who wants it. As I see it, the large LIS companies got caught flat-footed by the desire of histology labs to move to barcoding. Indeed, when I worked for a company that manufactured immunostainers we approached several large LIS companies about integrating our bar-code-driven instrument with their systems (we had already prototyped it in our lab with our own mockup of an LIS). I kid you not, the response of each and every one of them was Why would you want to do that? Next, they said they could do it if we had a customer that would pay for it. Here in Silicon Valley, where products are developed on the premise that people don't know what they want until you give it to them, that's like admitting you are clueless. Most of these LIS companies are not very proactive and are largely
RE: [Histonet] RE: MOHS IPs
Thanks for the reply, We are pre-fixing in a formalin substitute, but we'll give the acetone a try. I have diluted the protease, but have not gotten any good results. We'll keep trying with that as well. We've tried multiple incubation times for the cytokeratin... From 5 minutes all the way up to 30 minutes... With no luck. We are using an enhanced polymer DAB. Thanks for the suggestions! I greatly appreciate it! :) Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Wednesday, October 02, 2013 9:36 AM To: Barbara Tibbs; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] RE: MOHS IPs I agree with Barbara try fixing in cold acetone/ethanol mix even = just for a couple of minutes then go directly to buffer do not dry after=x, I would dilute the protease 1:4 with buffer (PBS or TBS what ever u u=) and do it for a short time. Enzyme digestion on frozen sections i=ricky but fixing a little and diluting the enzyme should help prevent ov= digestion. How long are u incubating the primary? Are u usin=n enhanced labeled polymer detection? HRP/Dab or AEC or Alk.P/fast=d? Original Message Subject: [Histonet] RE: MOHS =s From: Barbara Tibbs [1]barbara.ti...@accuratediagnosticlabs.com D=e: Wed, October 02, 2013 5:54 am To: Bauer, Karen L. [2]bauer.ka...@mayo.edu, 'histonet@lists.utsouthwest=n.edu' [3]histonet@lists.utsouthwestern.edu Hello Karen, =A Back in the old days of doing IHC manually on mostly fresh, froze n tissue I would immediately place the slide with the frozen section into a=plin jar of acetone to fix it. Keep the coplin jar of acetone in the cr=stat. Leave it in the acetone for only 10 minutes. Try diluting the Pr=ease 2 - 1:1 to make it gentler so it doesn't eat the tissue. This te=nique worked well when I was doing ER/PR on frozen breast tumors. Not su= it will work with skin but it's worth a try. Barbara S. Tib= Histology Supervisor Accurate Diagnostic Labs South Pl=nfield, NJ [4]barbara.ti...@accuratediagnosticlabs.com 732-839-3374 C=l: 610-809-6508 _=_ From: [5]histonet-boun...@lists.utsouthwestern.edu [6]histonet-bounces@lists.utsouthwestern=du on behalf of Bauer, Karen L. [7]bauer.ka...@mayo.edu Sent: Tuesday, October 01, 20 5:22 PM To: '[8]=sto...@lists.utsouthwestern.edu' Subject: [Histonet] MOHS IPs Hi all, We are in the process of validating some a=ibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, =t it could be darker. We will be increasing the Ab incubation time to se=f that will help. As for the Pan Keratin, we cannot get it = work at all. We use Protease 2 on our Ultra stainer for FFPE tissues in=stology, but this stain in MOHS is placed on fresh, unfixed tissue... by=anual drop method. Any time we've tried to use an enzyme retrieval, th= tissue looks eaten up... even if we incubate if for a minute. =ASince the tissue is not fixed, I figured that no retrieval step would be=eded, but the Pan Keratin refuses to work with or without retrieval. =A For those of you in MOHS labs that are using a manual staining me thod for Melan A and Pan Keratin, would you be willing to share your protoc= with us? Thanks so much!! Karen K=en L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab S=ervisor | Dermatology | Phone: 715-838-3205 | [9]bauer.ka...@mayo.edu[10]mailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 122=hipple Street | Eau Claire, WI 54702 | [11]mayoclinichealthsystem.orghttp://www.[12]mayoclinichealthsystem.o rg/ =A ___ Histonet ma=ing list [13]Histo=t...@lists.utsouthwestern.edu [14]http://lists.utsouthwestern.edu/mailman/l=tinfo/histonet _= Histonet mailing list [15]Histonet@lists.utsouthwestern.edu [16]http://lists.utso=hwestern.edu/mailman/listinfo/histonet References 1. 3Dmailto:barbara.tibbs@accurated 2. =mailto:bauer.ka...@mayo.edu; 3. 3Dmailto:histonet@lists.utsouthwestern.edu 4. 3Dmailto:barbara.tibbs@accuratediagnosticlabs.c 5. 3Dmailto:histonet-boun...@lists.utsouthwestern.edu 6. 3Dmailto:histo 7. 3Dmailto:Bauer.Karen 8
[Histonet] MOHS IPs
Hi all, We are in the process of validating some antibodies in our MOHS lab. The Melan A (Mart 1) antibody is working well, but it could be darker. We will be increasing the Ab incubation time to see if that will help. As for the Pan Keratin, we cannot get it to work at all. We use Protease 2 on our Ultra stainer for FFPE tissues in Histology, but this stain in MOHS is placed on fresh, unfixed tissue... by a manual drop method. Any time we've tried to use an enzyme retrieval, the tissue looks eaten up... even if we incubate if for a minute. Since the tissue is not fixed, I figured that no retrieval step would be needed, but the Pan Keratin refuses to work with or without retrieval. For those of you in MOHS labs that are using a manual staining method for Melan A and Pan Keratin, would you be willing to share your protocol with us? Thanks so much!! Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blade Rationing
Wow!!! It is pretty obvious this person has never worked as a HT. There are somethings you can cut corners but somethings you cannot. Do they want unreadable slides.I would without hesitation say to this manager the reason why this is not a good idea and would he want substandard slides if it was his tissue or someone he loves. Augh Stuff like this just makes me angry. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teresa Moore Sent: Monday, June 17, 2013 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Rationing I work in a hospital, there are three of us on this particular shift and we cut approx. 200 blocks, give or take a few. Our histo lab manager is telling us we should only be using one pack of blades (50 per pack) a month. I'm wondering what other techs think of this especially lab managers and supervisors. tmoor...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening
We have a job opening in our dermatopathology lab for a Histotechnician. If interested, please submit resume to: Westmoreland Dermatology P. O. Box 8695 Columbus, MS 39705 or email kvick...@westmoreland-derm.com Thank you, Karen Vickers Westmoreland Dermatology Administrator phone 662-243-2435 fax 662-328-7037 cell 662-425-3769 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pregnancy
I was working in a Histology Lab when I first got pregnant. I told my doctor about everything I was exposed to in the lab. He actually opted on putting me on medical leave (workman's comp). I did this with both of my pregnancies. Not in less you can find her a job that she is not exposed. I would not risk exposure. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne Sent: Tuesday, March 05, 2013 10:10 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pregnancy Hi Gang, Thought I would ask some people with some more experience than I in this. One of our techs is pregnant- Yay !!! She is very early - about 5 weeks. My Lab Director wants her to start wearing a mask due to the chemicals. So we need to ask what sort of precautions need to take place during the pregnancy etc. We also coverslip by hand here out of xylene etc. I can not remember ever working with any pregnant HTs in the past so I honestly do not know. Please carbon copy replies to jascholefi...@skaggs.net as well. We predominately work with 10% formalin, reagent grade alcohols, paraplast, xylenes, clear-rite 3, Decal I and II (from Surgipath), cytofixative and some routine special stain items. Basically the typical myriad of stuff a very small routine histo lab would have (no immunos) Thanks and bunch for your help ! Sincerely, Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 107, Issue 14- CLEANING MOLDS
Good Morning Bill, We clean our molds once a week via the purge cycle in our tissue processor. They come out very clean and ready for a release spray after which they are stacked and placed back into the embedding unit. The molds are used for the week. We alternate the cleaning cycles amongst the embedding centres so that all molds are not being cleaned at the same time. This system has worked well for us and has eliminated much of the manual aspect of performing this task. It also greatly lessens the possibility of any small fragments of tissue that could be left behind if the tray is removed before the block has had long eough to totally cool. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital -South Zone West - Alberta Health Services 960 - 19 Street South Lethbridge, Alberta - Canada T1J 1W5 Phone: (403) 388-6061- Fax: (403) 388-6067 karen@albertahealthservices.ca Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, October 08, 2012 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ergonomics
Hi Everyone, Has anyone ever used the adjustable microtome handle from Newcomer Supply it is called, Histo comfort grip handle? If you have, what did you think? How many of you are using a foot pedal if your microtome comes with it. I am getting repetitive motion stress in mainly my shoulder and a little in the elbow and wrist. I guess that is what happens as we get older and have been doing this for a long time. I have used my foot pedal a couple of time but then I lose the feel when I am facing into the block and sometimes finessing a difficult ribbon. Your thoughts on this would be greatly appreciated. Thanks Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Productivity
Good Morning, I know we have been over this before, how does your lab measure productivity? Are you going by block and slide or by CPT code? I have been told by the Suits that we can only measure productivity by per specimen or CPT code with no weight and value. As you know when you get a big case you can have a bunch of blocks on it therefore a bunch of slides. I do everything from GI specimens to big breast cases. They will tell me that our productivity is down and we need to cut hours. I just do not know exactly how to drill it in to them that things are not always as they appear on paper. I am talking in circles until I am blue in the face...AUGH!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processor
I am going to need to purchase a new tissue processor mine keeps breaking down. What tissue processor would you buy and why? I would greatly appreciate the help. Cheers, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MOHS Tech
Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org http://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P63
I guess since Biocare cannot sell their P63 antibody who is carrying it then? Dako, Ventana etc? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Please remove me from your mailing list. Karen E. Cruise Histologist (Research Technician II) Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Room 2332 St. Louis, MO 63110 (314) 454-8636 Phone (314) 454-5525 Fax kcru...@path.wustl.edumailto:kcru...@path.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Not requiring HT Certification)
I learned 22 years ago with OJT and then went and got certified a year later. I also love to teach. What I am finding now days is the new techs only know how to embed and cut but push buttons for everything else. To me they missed a very important step in their training. Very hard to find one that can actually manually do special stains. I personally like to do mine by hand because I think the quality is so much better. I know this is hard for very large labs. Just my two cents, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Friday, May 25, 2012 7:03 AM To: Mayer,Toysha N Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Not requiring HT Certification) I personally agree with your route. What I am most concerned with is so many want a quick route these days and don't want to put in the hard work to learn the theory. They just want to pass the test, get a job and make a big paycheck and expect the lab that hires them to actually teach them. I enjoy teaching myself to a degree but times are changing and we are expected to do more with less. We need graduating techs to be hit the ground ready day one. If we don't start to expect this you can count on with all the cut backs in health care that our profession will continue to be viewed as just a bunch of monkeys and therefore will be payed as a bunch of monkeys. And personally being refered to as a monkey ticks me off. I'm not a monkey. I'm a frog lol Sent from my iPhone On May 25, 2012, at 9:25 AM, Mayer,Toysha N tnma...@mdanderson.org wrote: Like everyone else, I was going to keep quiet, but I can't. I am sensitive to those downgrading us who took the OJT route. I did the OJT route, had a BS in Biology, tried for Veterinary School, but that wasn't so. What do I do with all of this Science? As part of my Pre-Med curricula had to take histology, loved it. Had no idea that it was a paying field and such. Had I known, I would have applied to a school as a backup. It took me a while, but when I was hired as a tech, I did not excel quickly (Cheryl remember my mistakes), but I learned and had patient coworkers. Move on down the line several years and another coworker questioned my knowledge of the chemistry behind a stain, and all of a sudden all of my organic and biochem can running out of my mouth. I didn't realize that I really knew all of that. I had always felt a little disadvantaged because I did not get formal training through a school, just studied and passed the HT with the help of my coworkers (shout out to LSU VetPath). There is nothing wrong with OJT for Biology majors. They should have the basic background to understand the chemistries and processes behind why we do what we do. With the modernization of technologies and procedures in the histo lab some formal education is needed. There are many ways to receive this education, it can be online, or in person. It all depends on the learner. To overcome the stigma we should continue with some of the things that are now in place to stabilize the training of our successors (yes we all are going to have to retire one day). A continued push for formal training, promotion of the field, professionalism by our colleagues, and respect from the customers (pathologists, patients, and gen lab personnel). In order to facilitate change for respect, we must first present a unified front. I know good techs with no certification, I know bad techs with certification. We all do. Last year I celebrated my 20th year in histo and never thought I would be where I am. I never wanted to do research, and didn't like management (don't like telling grown folks what to do), but I love teaching. It helps me to learn the theory behind what I do and apply it better. There are so many people who can do histo whether it is routine, special procedures, or management. We shouldn't look down on those who took
[Histonet] DeCloaking Chamber
Hi, Does anyone know of a delayed start Decloaking chamber that is independent from the IHC stainer? I am looking around to get a new Decloaking chamber. Any suggetions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Productivity ReportsMarcia Funk
I have been battling this for years they gives us RVU's for each task. What a headache. The problem I run into is they only see per specimen. If I have a specimen that has 30 cassettes it does not matter it is treated the same as one cassette per specimen. Not to mention the doctors will hold cases for the next day or two and then one day I get slammed with a 120 cassettes and only have maybe 30-40 specimens from the regular surgical from the day before. You have to make them realize that this it is just not as cut and dry as it looks. I do not like when they just look at the numbers and graphs and not the real workload. I am the Chief Cook and bottle washer here and they still get on me about productivity. I agree with Tim on the labs doing things differently and different specimens so it is hard to compare. I feel your pain. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, May 01, 2012 9:01 AM To: Marcia Funk; Allison D Scott; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Productivity ReportsMarcia Funk One issue to consider is that you need to find a way to compare apples to apples. Generally that means doing workload by comparing identical shifts with identical work practice, or doing your workload recording in small increments so you are only measuring one task at a time. For instance, while several people may be scheduled to section they may also be assigned additional work like changing processors, backing up special stains, doing the daily rush biopsies, doing some data entry. Along with all that the sectioning should be the same kinds of cases - bx vs bx, etc. All those can skew the sectioning totals in various ways. You need to normalize the counts so they are all measuring the same work in the same way. Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Funk Sent: Tuesday, May 01, 2012 8:44 AM To: Allison D Scott; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Productivity Reports Scott, I'm in the same process you are going through. Please share with me if you receive feedback. Thanks Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 Scott, Allison D allison_sc...@hchd.tmc.edu 04/30/2012 4:24 PM Hello to all in histoland. Does any one have to do productivity reports for their boss. If so, what are you using for your unit of measure to say if your techs are being productive. My boss is really into the numbers, graphs and charts. I have a my techs fill out a workload recording form. I really don't know where to begin trying to pull data from it for a report. If anyone has any insight to this and has a report that they would be willing to share, I would be eternally grateful. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman
[Histonet] Pinning Specimen
Does anyone know where to get specimen boards that you can pin specimens to and then submerse in formalin? I ordered them a long time ago and cannot remember where I got them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN4
I am trying to work up the PIN4 cocktail. Can anyone out there help me with a procedure? We use the Benckmark Ultra. I have the Ventana basal Cell Cocktail and the P504s from Cell Marque that I was told by my Ventana rep to order. All help is appreciated!! Karen S. Gary HT (ASCP), BBA Laboratory Manager Confidentiality Notice: The information contained in this transmission may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately notify me by telephone and permanently delete the original and any copy of this e-mail and destroy any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Neurofilament - LFB combo -- problems
To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana Benchmark: I have been trying to stain a nerve (cross and longitudinal section) and it keeps falling off of the slide after the neurofilament stain. It's been cut onto a charged slide and has been baked 20 minutes prior to IHC staining. After IHC staining, we have rinsed in Dawn-water per protocol; by then the longitudinal section is peeling off the slide. I tried skipping the Dawn rinse and have gone to straight 95% alcohol and 1 of 4 sections survived on the slide (others fell off). Tissue problem - the nerve is well myelinated and fatty. If I drop the water bath temp too low, the nerve doesn't flatten well; if the temp is too high, it's shredding and spreading. Has anyone out there had this problem and resolved it? Or do you have a protocol that might have avoided it completely? Any suggestions would be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Position
Seeking a qualified histology tech for full time position. Private pathology lab located in East Texas. Monday - Friday 6:30 - 3:00. Please send resume to kareng...@mplpath.com for more detailed information. Karen S. Gary HT (ASCP), BBA Laboratory Manager Medical Pathology Lab 402 N. 5th Street Longview, TX 75601 903.758.8511 ext. 201 903.758.8528 Confidentiality Notice: The information contained in this transmission may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately notify me by telephone and permanently delete the original and any copy of this e-mail and destroy any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parameters for alcohol on Processor
Hello Histonetters, Could someone please advise me on established guidelines for changing the alcohol on the processor. We process a very small amount of blocks weekly on our ASP300S. Approximately 20 blocks per week. We have decided to use a hydrometer to test our 70% thru 100% alcohols, rather than base our changing reagents on number of blocks processed. My questions are, does a (1) % point decrease necessitate a change ? What are the parameters for changing each alcohol ? Thanking you in advance, Karen Karen E. Cruise Histologist (Research Technician II) Washington University School of Medicine Laboratory for Translational Pathology 216 S. Kingshighway Room 2332 St. Louis, MO 63110 (314) 454-8636 Phone (314) 454-5525 Fax kcru...@path.wustl.edumailto:kcru...@path.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Music in the Laboratory
I could not work with out music. Nothing like cutting to the rhythm of good music. I have it on all the time or I would go nuts and probably fall asleep. I set my radio to one radio station. I am the Chief Cook and bottle washer so no one to bother with the music. If someone wants to play hard core obscene music let them put it on their Ipod so not everyone can here it. I would make a rule that they have to be able to hear if someone is talking to them. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tracz...@aol.com Sent: Tuesday, January 17, 2012 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Music in the Laboratory Greetings. I would like to know what other histology laboratories allow for music players while working. Do you have formal policies about music content or volume? Do you allow lab space doors to remain closed to muffle the volume of what is being played? Are headsets allowed? I am a terrible judge of this because I personally prefer to work in a quiet environment. I am trying to be open minded, as long as the work gets done. However, one of the techs had a song playing today that I believe was inappropriate for general listening in the lab. Am I just out of touch? Is that dang F word just something I'm going to have to learn to accept? Do you have a written policy? When/how/why was it implemented? I should mention that it's a small private lab, with minimal patient traffic. We do see our share of FedEx, UPS, sales service reps. Your ideas on this is very much appreciated. Dorothy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pms 2 antibody trouble
We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, Freeman, Carol carol.free...@utoledo.edu wrote: I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but nothing is consistent. We are looking to try another vendor's antibodies, but were hoping for some feedback as to which labs have found success in this area. I have never had trouble like this with an antibody. The severe problem here is that with MSI testing, we are looking for lack of staining with this antibody to show a positive result so light patchy staining leaves a lot of room for error. Any responses are appreciated. Thank you, Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.free...@utoledo.edu (419)383-5639 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] time off
Hi Amber, I have a Holiday Rotation List that I use for my staff. I started the list off with seniority, but then everytime a tech uses PTO around a holiday (either before or after), they go to the bottom of the list for that holiday. This ensures that all techs have a chance to take off and it's equal for everyone. There are some techs that ask for holidays every year, but I post the holiday rotations and they know they might not get it. Even though they asked 6 months in advance, they have to wait to see if the techs at the top of the list will be utilizing PTO for that particular holiday. If not, they can have it off... And then go to the bottom of the list again. I update the rotation list every year and post it... It's helped a lot with holiday fairness. I can send you the form if you would like... Just so you can see what I'm talking about. Hope this helps, Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor - Pathology Department MOHS Lab Supervisor - Dermatology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 03, 2012 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] time off Those of you who are supervisors, how do you handle your co-workers asking for time off? I have 2 employees that have asked off already (jan 3rd) for every day they want off for the entire year! Do you grant them the days off since no one else has asked off yet, or tell them it's not fair to continuously get off around every holiday by asking off 5 - 12 months in advance? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] immuno stainers
We have the IntelliPath as well. I have the same sentiments as Linda regarding all aspects of Biocare and the instrument. We use their antibodies and detection. The company as a whole is excellent to deal with and very customer oriented. Thanks, Karen Lahti Arizona Digestive Health Phoenix, AZ On Dec 27, 2011, at 12:58 PM, Blazek, Linda lbla...@digestivespecialists.com wrote: I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel. Linda Blazek GI Pathology 937 396-2623 Sent from my iPhone On Dec 27, 2011, at 11:51 AM, Cynthia Pyse cp...@x-celllab.com wrote: Hello Histonetters Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how times flies when you have time off. Now it's back to the old laboratory grind. We are in the market for a new immuno stainer. I currently use a Dako Link, which I am very pleased with. I have demoed the new Dako with great results, unfortunately we need 2 stainers and the pricing maybe a little out of our range. Is anyone using the Biocare Intellipath? What are the pro's and con's? How is their service? I currently use their detection system, Mach 4, but the majority of my antibodies are purchased from Dako. Any input on the daily use of the Intellipath from current users would be appreciated. Thanks in advance for the input Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabbit antibody on rabbit tissue
Hi All, I am going to apply one rabbit polyclonal antibody on the rabbit tissue using IF. Is there anybody do it before? I heard of mouse on mouse IHC-DAB method, but not sure if it's possible in IF. Your kind response is very appreciated, Thanks, Karen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: H. Pylori - IHC
We use Biocare antibody with great results on the IntelliPath from Biocare and the Bondmax from Leica. Karen Lahti Arizona Digestive Health -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, November 17, 2011 6:30 AM To: 'beth@hcahealthcare.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H. Pylori - IHC We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of beth@hcahealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 mailto:beth@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris Rapid tissue processor
Hi Muhammad At our lab we have a Peloris, and it's a great automated tissue processor. You'll get good results by the factory protocols but do also have the posibillity to create you own protocols for the tissue you are running most. That is what I do. I'm using the xylene substitute Tissue Clear and am running mostly fatty tissue. I'm almost using the factory time for ethanol but have extended the clearing and wax step a bit. I get really good results, and it's great to have this opportunity. A thing more I like about the Peloris is that you are not dependent of a specific company to sell you the reagents, and the Peloris is really easy to operate with. The ergonomics is also great. I look forward to get a Peloris more someday. Regards Karen Slagelse pathology Department Denmark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana Eber Probes
Hi Andria, This is our EBER protocol that we are using on our XT... With great success. Using the XT ISH Open Probes - Chromogenic procedure... Depar Enzyme: ISH Protease 3 for 12 minutes Probe HybReady Probe Auto Dispense: Then pick the probe that contains EBER Denature: 60 degrees C for 4 minutes Hybridization: 37 degrees C for 1 hour Stringency Washes Stringency Wash #1: 47 degrees C for 8 minutes Stringency Wash #2: 8 minutes Stringency Wash #3: 8 minutes Detection Kit Blue Detection Since Default is iView Blue+, just put 32 minutes in for the Substrate Incubation Time Counterstain: Red Stain II for 4 minutes This protocol has worked well for us. We save our Ultra for routine IPs throughout the day and only use the XT for ISH runs, since they take longer. Good Luck, Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire Phone: 715-838-3205 E-mail: bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Evans, Andria B Sent: Friday, November 04, 2011 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Eber Probes I am struggling with my EBER probe on both the Ventana Benchmark XT's and Ultra. I was hoping that someone could help me out with what you are currently running with the new probe setup. Thank you! A. Harris (717)544-5457 ~ FAX (if it would be easier to fax the protocol) This email was sent securely from the LGHealth Email Service Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Urgent!! Need per diem
I am looking for a Certified ASCP HistoTech. that is local to the San Francisco Bay Area. I need this person to cover me while I am out having knee surgery. I will be out 2-6 weeks probably more like 4 weeks starting October 5th. But need somebody by October 3rd to do some training. You will need to know IHC and Special stains and be able to work independently because I am the Chief, Cook and Bottle washer here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Traveling HistoTech San Francisco Area
Hello, I am going to need a ASCP certified Histotech to work for me for about 1.5-2 weeks in the next 2-4 weeks. If you now of a temp. service or someone interested. Call me and leave a message and will get back to you. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bovine corona virus antibody
I am looking for antibody recommendations for our bovine corona virus IHC. The clone we used previously is no longer available. Thanks, Karen Sverlow, HTL (ASCP)CM Histology Supervisor California Animal Health and Food Safety Laboratory System Thurman Building, West Health Sciences Drive Davis, CA 95616 530-752-8753 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RECYCLERS - RE: Histonet Digest, Vol 94, Issue 7
Good Morning, We use the CBG recycler and are very happy with both the performance of the instrument and support provided by the company. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital South Zone West - Alberta Health Services Lethbridge, Alberta,CANADA karen@albertahealthservices.ca Message: 5 Date: Tue, 06 Sep 2011 12:38:36 -0700 From: Behnaz Sohrab sohra...@ah.org Subject: [Histonet] Recycler To: histonet@lists.utsouthwestern.edu Message-ID: 4e66144c.4347.005...@ah.org Content-Type: text/plain; charset=US-ASCII We are in the market to buy recycling system, any recommendation between CBG or B/R system ?? Any suggestions is deeply appreciated. Thanks Behnaz Sohrab,Manager Anatomic Pathology This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problems posting
I am having problems posting to Histonet. Not sure what is going on. Hopefully this goes through if not please add me to your list. I have been on the the Histonet for 7 years now. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 616 7Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Per diem Position in the San Francisco Bay Area
We are looking for a Certified Histology Technician for per diem work. You will also do some Pathology Assisting (nothing to extensive on the PA). Need someone that can cover vacation, sick days, etc. Perfect for someone that is retired and is looking for some extra money. Need to be able to work on your own independently and know how to troubleshoot both Histology and IHC's. You will need to be able to be available to work up to 1-2 weeks at a time or get called in at the last minute in case someone is sick. So a flexible schedule is ideal. If interested please call and leave me a message. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 616 7Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MOHS daily flow...
Hello, This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] MOHS daily flow...
Hi Jeanine, Our patients go back to a MOHS only waiting room between layers. Nice comfy loungers, big screen TV, refreshments, things to make them comfortable while they wait, since it could be a long day for them. They can bring in one family member with them if they want. Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org -Original Message- From: Bartlett, Jeanine (CDC/OID/NCEZID) [mailto:j...@cdc.gov] Sent: Tuesday, July 26, 2011 12:53 PM To: Nicole Tatum; Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MOHS daily flow... That is very interesting. A friend had a procedure done last week and they kept him on the table until they were done. he did not go back to a waiting area. Is this common? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Tuesday, July 26, 2011 1:40 PM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] MOHS daily flow... Importance: Low Karen The number of patients depends on the surgeon. Some do 4-15 per day. It also depends on how many rooms you have to see patients. For the most part, Lets say you had two rooms available. and had 6 patients scheduled. schedule pt 15-20 mimutes apart. Bring first two in at same time. Doctor can get layers bandage pateints and place them in a waiting area. Next two patients come in get prepared and layers are taken. Place in waiting area. Next patients are brought in. layers taken. Place in waiting area. Each specmen after layer is taken is brought in lab. Mohs tech should be able to complete each case within 15-20minutes. Slides are placed for doc to read. Once he has taken all layers he can begin to read ready cases and take layers or begin closures. Pateints layers are taken and then they are placed back in waiting areas. This will continue until are patients are done. Tissue is grossed before it is placed in cryo. Two slides should be cut per peice of tissue. Not per case. We place 3 sections on each slide. Each section is a little deeper then the one before. We start at the frosted end. A great fast effecient tech should be able to handle 10-15 cases per day. I hope this helps. Nicole Tatum HT ASCP This is for those techs that assist in the MOHS lab... What is your daily flow for the work performed in your lab? How are patients usually scheduled? (Are they staggered? All come in at approximately the same time so 1st layers can be taken right away?) How often are you receiving patient specimens? Usually, how many patients per day? Do you gross the tissue before freezing? What is the goal time for slide completion? (From the time the tissue is placed in the cryostat to freeze to the slide being ready to stain.) How many levels are you placing on the slide? How many slides per block are you cutting? Do you have a set time limit on when all first layers are to be done? Any additional information is greatly appreciated. Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Pathology Department Mayo Clinic Health System in Eau Claire E-mail: bauer.ka...@mayo.edu mailto:bauer.ka...@mayo.edu ___ Mayo Clinic Health System 1221 Whipple St. Eau Claire, WI 54703 www.mayoclinichealthsystem.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
