Re: [Histonet] HP antibody
We have used Biocare’s Hp concentrate and predilute for years. Very consistent and the concentrate is strong. There is no background and it is very clean. Karen Lahti, HT, QIHC Lab Manager Arizona Digestive Health Phoenix, AZ > On Apr 25, 2017, at 5:37 AM, Pairan, Kelly via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > Hello Histoworld, > Happy Lab Week! We have not been able to get H. pylori antibody from our > current supplier for a while. Who is the supplier for your lab and do you > have any stock issue? > > Thanks and have a great day! > Kelly Pairan, HT (ASCP) QIHC(ASCP) > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana H.Pylori antibody
Hi Julia, We have a GI lab and use Biocare's H. Pylori concentrate on Biocare's IntelliPath. We run about 50-75 H. pylori IHC each day. They are very clean and specific. Our Pathologists and myself are extremely happy with Biocare customer service and products. On Thursday, July 30, 2015, Cates, Julia via Histonet histonet@lists.utsouthwestern.edu javascript:_e(%7B%7D,'cvml','histonet@lists.utsouthwestern.edu'); wrote: Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to clean up the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253- ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Thank You, Karen Lahti, HT ( ASCP), QIHC 480-236-6559 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: proceesing of GI Bxs
We have a medium GI lab using the old, old VIP's. We have been very successful with two processing schedules, long and short. We found that we needed to add a bit of heat to the last xylene so when the tissue hit the first paraffin, they would start to infiltrate. The cold cassettes solidified the paraffin and the time spent was melting the paraffin not infiltrating the biopsy. For the longer run, we add 10%NBF and a bit longer in each solution. Short: 1. 70% reagent alcohol - 15 min, 40C, P/V 2. 85% reagent alcohol - 10 min, 40C, P/V 3. 85% reagent alcohol - 15 min, 40C, P/V 4. 100% reagent alcohol - 10 min, 40C, P/V 5. 100% reagent alcohol - 10 min, 40C, P/V 6. 100% reagent alcohol - 10 min, 40C, P/V 7. Xylene - 20 min, 40C, P/V 8. Xylene - 20 min, 50C, P/V 9. Paraffin - 10 min, 58C, P/V 10. Paraffin - 30 min, 58C, P/V 11. Paraffin - 45 min, 58C, P/V Karen Lahti, HT (ASCP), QIHC Arizona Digestive Health 602-687-7468 On Apr 3, 2014, at 9:33 AM, Arguello, Daniel arguel...@ihn.org wrote: I'd appreciate a copy of that processing schedule for GI bxs as well. Thank you in advance Sincerely, Daniel Argüello, BS, CT (ASCP)CM Anatomic Pathology Services Coordinator Inspira Medical Center Woodbury 509 North Broad Street Woodbury, NJ 08096 856-853-2030 Ext 2808 856-853-2183 Fax arguel...@ihn.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vicki McKaughan Sent: Thursday, April 03, 2014 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] proceesing of GI Bxs Could someone please send me your processing schedule for GI bxs. We are using a Sakura VIP5 and are having problems with over drying and occasionally with infiltration of paraffin. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pms 2 antibody trouble
We use the Biocare concentrated antibody on the Biocare Intellipath. We are diluting at 1:100 and have very good consistent staining. Give me a call if you want to discuss further. Karen Lahti, HT, QIHC Arizona Digestive Health 602-687-7468 On Jan 12, 2012, at 9:42 AM, Freeman, Carol carol.free...@utoledo.edu wrote: I am curious if anyone is having trouble validating PMS2 antibody for the MSI panel. We have worked this antbody up extensively and we are finding that it is on either side of the spectrum just too light (it is either light staining if any at all). We have stained a few cases beautifully but nothing is consistent. We are looking to try another vendor's antibodies, but were hoping for some feedback as to which labs have found success in this area. I have never had trouble like this with an antibody. The severe problem here is that with MSI testing, we are looking for lack of staining with this antibody to show a positive result so light patchy staining leaves a lot of room for error. Any responses are appreciated. Thank you, Carol E. Freeman HTL (ASCP) B.S. Department of Pathology University of Toledo Medical Center 3000 Arlington Avenue Toledo, OH 43614-5807 carol.free...@utoledo.edu (419)383-5639 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] immuno stainers
We have the IntelliPath as well. I have the same sentiments as Linda regarding all aspects of Biocare and the instrument. We use their antibodies and detection. The company as a whole is excellent to deal with and very customer oriented. Thanks, Karen Lahti Arizona Digestive Health Phoenix, AZ On Dec 27, 2011, at 12:58 PM, Blazek, Linda lbla...@digestivespecialists.com wrote: I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel. Linda Blazek GI Pathology 937 396-2623 Sent from my iPhone On Dec 27, 2011, at 11:51 AM, Cynthia Pyse cp...@x-celllab.com wrote: Hello Histonetters Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how times flies when you have time off. Now it's back to the old laboratory grind. We are in the market for a new immuno stainer. I currently use a Dako Link, which I am very pleased with. I have demoed the new Dako with great results, unfortunately we need 2 stainers and the pricing maybe a little out of our range. Is anyone using the Biocare Intellipath? What are the pro's and con's? How is their service? I currently use their detection system, Mach 4, but the majority of my antibodies are purchased from Dako. Any input on the daily use of the Intellipath from current users would be appreciated. Thanks in advance for the input Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: H. Pylori - IHC
We use Biocare antibody with great results on the IntelliPath from Biocare and the Bondmax from Leica. Karen Lahti Arizona Digestive Health -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, November 17, 2011 6:30 AM To: 'beth@hcahealthcare.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H. Pylori - IHC We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of beth@hcahealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 mailto:beth@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare Intelipath
Sheila, I have been a long time Biocare user, as well as many years of experience with just about every IHC company/instrument on the market today. I have had a fair share of issues with each. I am amazed continuously at the attention and care I am given from the whole Biocare company. When I call with even a minor maybe problem, I do feel as if I am their only customer. I get immediate response. The IntelliPath instrument does require more tech time to prepare the slides which I resisted from the very beginning. I have learned the flexibility this has brought to validation and overall quality of staining. The antibodies and detection are exceptional in comparison to other vendors. The detection coupled with the instrument enabled our lab to drastically reduce the cost of each stain and keep a high level of quality that is reproducible from day to day. Karen Karen D. Lahti, HT(ASCP)QIHC, MLT(AMT) Histology Supervisor Arizona Digestive Health 1300 N. 12th Street #550 Phoenix, AZ 85006 480-236-6559 cell 602-687-7218 office -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Sunday, May 08, 2011 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Intelipath Hello Netters We are considering an Intelipath. I'd like some opinions on the instrument from users out there. Thanks in advance!!! Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CIMP
I am looking for a lab I can reference some slides to for CIMP (CpG Island Methylator Phenotype). Karen D. Lahti, HT(ASCP)QIHC, MLT(AMT) Histology Supervisor Arizona Digestive Health 1300 N. 12th Street #550 Phoenix, AZ 85006 480-236-6559 cell 602-687-7218 office ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
