Re: [Histonet] floor vibration
I can think of two things. First, relentlessly make fun of your building planner for putting a histology lab underneath a laundry. This is a mistake worthy of pointing and laughing. Second, there are isolation tables and platforms. That's probably the first thing I would try. http://www.taab.co.uk/pdf-details/305_taab_products_1402580607.pdf Gerry -Original Message- From: Nancy Schmitt via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 10:03 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Aqueous Mounting Media
The slides are an important part of the medical record. Sure… I could assure that slide that are covered with media only are well treated while in my custody. But, those slides might see all sorts of abuse once they are outside of my care. They have to survive whatever pathologists, surgeons, residents, or fellows can throw at them. I feel better having a sturdy piece of glass over those sections. Gerry From: Caroline Miller [mailto:mi...@3scan.com] Sent: Wednesday, November 04, 2015 7:05 PM To: Keyser Gerald T Subject: Re: [Histonet] Aqueous Mounting Media Is there a reason you have to coverslip? I am presuming for storage. But I have found that the aqueous media alone is fine for imaging / viewing. If you are careful storing them they should be fine too. I use this: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles=MMC0619 and I take a piece of parafilm, make sure the slide is wet, but not dripping (I take it out and give it a quick flick) put a drop of the aquaslip on the slide and use capillary action to pull it across the section, you have to be careful not to scratch the sample (make sure there are no nicks in the parafilm). It gives a nice flat surface, but it sounds like you are also getting some off-gassing from the product. Oh Science! Good luck! mills On Wed, Nov 4, 2015 at 6:08 AM, Keyser Gerald T via Histonet <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> wrote: Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at replacing it with a different Aqueous mounting media. The only stain we use it for is Oil Red O. I've tried many different variations on its use and the results have been the same. 1) The slide looks lovely the day that the coverslip is mounted and then dozens of bubbles form after. Sealing does not make a difference. Following company recommended procedure doesn't make a difference. Getting creative doesn't make a difference. Nothing seems to make a difference. 2) We use a non-aqueous media surrounding the aqueous and post-coverslip the slide. This option is messy and cannot be re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure that this wouldn't work at all with a more delicate stain. Ok. I give up. Can anyone make a recommendation for a different aqueous mounting media? Gerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Aqueous Mounting Media
Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at replacing it with a different Aqueous mounting media. The only stain we use it for is Oil Red O. I've tried many different variations on its use and the results have been the same. 1) The slide looks lovely the day that the coverslip is mounted and then dozens of bubbles form after. Sealing does not make a difference. Following company recommended procedure doesn't make a difference. Getting creative doesn't make a difference. Nothing seems to make a difference. 2) We use a non-aqueous media surrounding the aqueous and post-coverslip the slide. This option is messy and cannot be re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure that this wouldn't work at all with a more delicate stain. Ok. I give up. Can anyone make a recommendation for a different aqueous mounting media? Gerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fixation of frozen section
Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation of frozen section Hi histonet, Would like to query about which fixative do you prefer for fresh frozen section slide before HE staining? Thanks for your advices Manahil HTL/ ASCP Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fixation of frozen section
We get good results with 95%. Unless prepping sample for Electron Microscopy, cell membrane preservation doesn't seem to be worth worrying about. It can't be resolved with a light microscope. A light microscope using a violet monochromatic light source should have the theoretical limits of resolution of approx. 200nm. Cell membranes... what do they run... approx. 4nm? Gerry -Original Message- From: Jamal [mailto:j.rowa...@alborglaboratories.com] Sent: Tuesday, April 28, 2015 12:07 PM To: 'Manahil'; Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fixation of frozen section You can't use full concentrate alcohol on such specimen, otherwise you will destroy the cells membrane. For me I am using 80% Ethanol. Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 7:23 PM To: Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fixation of frozen section What about the Absolute Methanol? Sent from my iPhone On Apr 28, 2015, at 8:16 PM, Keyser Gerald T gkey...@uwhealth.org wrote: Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation of frozen section Hi histonet, Would like to query about which fixative do you prefer for fresh frozen section slide before HE staining? Thanks for your advices Manahil HTL/ ASCP Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Looking for an article
I can't find it without a paywall either. I find the idea of honey as a formalin substitute fascinating. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, February 18, 2015 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for an article Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue. The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Covergrip
I would like to better seal my Oil Red O slides. The current process uses aqueous mountant on the inside of the large coverslips and Permount on the outside; which is incredibly messy and isn't actually that great of a seal. The two options I'm looking at is: 1) Nail polish. 2) Covergrip I would like to get away with using nail polish because it's 10% of the cost of Covergrip. We don't actually do a whole lot of Oil Red O stains. But, I'm a little concerned that the solvents in the nail polish may permeate the aqueous mountant and mess with the sections. Slides need to be good for 10 years. Opinions? Gerry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Solvent Recyclers
GRAC http://www.newcomersupply.com/product/gravity-recycling-alcohol-cartridge-tissue-processor-grac-tp I've used this system. It takes quite a while to run. It works. But, there is one big problem. If there is a possibility of Xylene or Propar contamination, it will quickly ruin the filter and cause problems with any system using the recycled ethanol. It doesn't take much to completely mess up the filter. But, it does have the possibility to save a lab quite a bit of money. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. Dependability more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathri...@pastnashville.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dragon Voice Recognition software
Voice to text cannot cope with doctors mumbling into recorders. Even the best VRS only hits about 95% of the regular dictionary; which is very good. But, a 5% error rate is wildly unacceptable in a clinical environment. Another parallel is trying to apply handwriting recognition software to a physician penmanship. Would totally make the software divide by zero and have a fit. gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Friday, September 26, 2014 3:59 PM To: Jay Lundgren; Houston, Ronald Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dragon Voice Recognition software The work that can be done in histology-pathology is endless! I know that in past positions that sometimes transcriptionists have felt threatened by voice recognition dictation and software when it was suggested. I don't really blame them for these feelings, but it never turned out the way they worried it might. Machines or computer software never completely replace people, they don't think, make decisions or have judgment. The people just have to function at a higher level or in an alternate way since the rudimentary processes for the people are reduced . I just set up Dragon dictation for the microscopic for the pathologists, but it will be in Orchard Pathology, so could not help with the interface to your system Ronnie. But it did not replace the information management function or its oversight, just changed the tasks that needed to be performed somewhat, and increased overall efficiency. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Fri, 26 Sep 2014 10:07:53 -0500 From: jaylundg...@gmail.com To: ronald.hous...@nationwidechildrens.org Subject: Re: [Histonet] Dragon Voice Recognition software CC: histonet@lists.utsouthwestern.edu I'd imagine it would really upset the transcriptionists, because you wouldn't need them anymore? On Thu, Sep 25, 2014 at 8:06 AM, Houston, Ronald ronald.hous...@nationwidechildrens.org wrote: Is anyone using this transcribing directly into Sunquest CoPathPlus? How successful was the transition, what problems did you encounter, and what resistance if any was there from pathologists, PAs and transcriptionists? Thanks Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto: ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MOHS
Embedding: I use a large, solid aluminum block that I keep cool in my cryostat. It's approx. 9 in (length) x 4 in (width) x 3 in (depth). I just stick the epidermal edge to the block and flatten it out so all margins are in contact with the freezing surface. Drop some freezing medium on it and attach it to a specimen holder. It's good to go. A large, thick, removable metal block as a freezing surface should be standard equipment for all cryostats. Sectioning: Having a liquid nitrogen gun to selectively freeze fatty constituents in the specimen is a good idea. After I collect the specimens on a slide I check it with a microscope with the light source turned on very low. That allows the tech to see if the epidermis is completely represented in the specimen. Other techs I work with do it a bit differently. But, the results I get from a quick examination under a microscope is excellent. Gerald Keyser -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, September 11, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MOHS We are looking at bringing MOHS in-house for a new physician. We already do a great deal of frozen sections, so we are set up for cryotomy and staining, however, I am in need of a good procedure for embedding, cryotomy etc. Thank you, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.al...@providence.orgmailto:lacie.al...@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades for cutting resin on a microtome
I've only cut resin with a glass or diamond knife in an ultramicrotome. If you are attempting to do it in a regular microtome, you would need a special blade holder. I don't know if any microtome manufactures make glass knife holders. You make the glass blades yourself using special glass. Here is a link to the glass strips: http://www.sigmaaldrich.com/catalog/product/sigma/g2528?lang=enregion=US Here is a cheap jig and diamond glass cutters it make the knifes: https://www.emsdiasum.com/microscopy/products/preparation/glassknife.aspx I've never made glass knives by hand using a hand held diamond cutter and jigs. I imagine that it would take practice. I've only used a specialized maker: https://www.emsdiasum.com/microscopy/products/histology/tissue_stainer.aspx You paint a bit of nail polish underneath the glass edge and put a bit of distilled water on the edge. You then section the block floating the sections on the water. Use an eyelash manipulator to pick up the 5um thick sections and place on a bubble of water on the slide. Evaporate the water droplet on the slide. If you've done it right, the sections won't look like origami. If it does, then practice until it doesn't. Gerry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Friday, September 12, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades for cutting resin on a microtome Happy Friday Histonetters! I am working on a histology platform in a research center and someone came to me last week and asked to cut blocs of resin (JB-4 resin) on the microtome. I never cut anything else than paraffin, so I was wondering if some of you had advices for me? They never did it neither and took their protocol in a paper where it was said that we should use disposable glass knife instead of standard metal blades. Are any of you ever used those knife? Where do you buy them? We have an old Leica RM2125. Thanks for your advices! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica Cryostat CM 1860 UV Decontamination Procedure
We use a Leica CM1850 currently. May differer a bit. On a daily basis, we clean the loose trimmings and wipe the inside with 95% ethanol. The exterior surfaces are cleaned daily with caviwipes. Once a month we turn off the machine, remove the microtome (I don't know if this is proper for the CM1860), let it completely defrost. Then clean the entire chamber with 95% ethanol. The microtome is dyed completely and the cryostat reassembled. This procedure was in place long before I started working where I do. I'm not sure if this procedure is something that is leica approved. I believe the CM1860 has a different microtome construction than the CM1850. I'm also hoping that the next cryostats we get have UVC. I really like the idea of UVC. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Adesupo, Adesuyi (Banjo) [abades...@nrh-ok.com] Sent: Wednesday, February 12, 2014 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Cryostat CM 1860 UV Decontamination Procedure Hi, How are you guys doing? Please I am wondering if some of you guys are using this type of Leica cryostat and would be interested in the decontamination procedure that you have in place. Thanks, Adesupo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Kisser's Mounting Media (Glycerol jelly)
I'm toying with making my own aqueous mounting media. Although I will not likely use this in actual cases, it would still be nice. Aqueous mounting media is kind of expensive. But, there are some questions that need to be answered. The Slides need to be kept for 10 years. - Will the adhesive yellow with time? - Will the adhesive continue to have complete coverage over time. Over drying? Cracking? - Since the mounting media is made from glycerin, gelatin, and water, will microbial invasion of the slides be a problem? I can add a anti-microbial agent to the recipe. Anyone have long term experience with this recipe? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
