[Histonet] Breast tissue with calcs

[Lima, Teresa via Histonet]

Hello Histonetters,
We are having issues with keeping micro calcifications in breast biopsy 
sections from falling off our plus slides. Any suggestions would be greatly 
appreciated.
Regards,
Teri

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[Histonet] X-ray machines for the lab

[Lima, Teresa via Histonet]

Hello all,
We are having issues with microcalcifications in breast tissue not being 
present in the H slides of breast biopsies. The pathologists would like to 
get an x-ray machine for the grossing room. Does anyone know where we can 
purchase a small, portable x-ray machine?
Thanks,
Terri

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[Histonet] FW: Ventana Optiview kit

[Lima, Teresa via Histonet]

Hello Histonetters,
Is anyone having issues with increased background staining of routine 
antibodies like keratins, CD markers and neuroendocrine markers using Ventana's 
antibodies with the Optiview kit? We are down to 4 minute antigen retrieval and 
primary antibody incubation times and still getting nonspecific staining. Our 
medical director thinks their prediluted antibodies are too concentrated for 
the kit. There are just a handful of their antibodies that are approved for 
this kit so there are no protocols in the package inserts. When we ran their 
breast markers with Optiview the positivity rate was incomparable to the 
results we get with UltraView and since the breast markers are not FDA cleared 
for OptiView we did not switch them over. It made our pathologists very 
apprehensive that with OptiView our Her2 validation cases became very positive 
and did not correlate with our UltraView stained slides and our FISH results. I 
look forward to your insights.
Thanks everyone!
Terri

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the intended recipient, please notify me immediately as use of this information 
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[Histonet] Ventana Optiview kit

[Lima, Teresa via Histonet]

Hello Histonetters,
Is anyone having issues with increased background staining of routine 
antibodies like keratins, CD markers and neuroendocrine markers using Ventana's 
antibodies with the Optiview kit? We are down to 4 minute antigen retrieval and 
primary antibody incubation times and still getting nonspecific staining. Our 
medical director thinks their prediluted antibodies are too concentrated for 
the kit. There are just a handful of their antibodies that are approved for 
this kit so there are no protocols in the package inserts. When we ran their 
breast markers with Optiview the positivity rate was incomparable to the 
results we get with UltraView and since the breast markers are not FDA cleared 
for OptiView we did not switch them over. It made our pathologists very 
apprehensive that with OptiView our Her2 validation cases  became very positive 
and did not correlate with our UltraView stained slides and our FISH results. I 
look forward to your insights.
Thanks everyone!
Terri

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[Histonet] Image analysis on breast markers

[Lima, Teresa via Histonet]

Hello all,
I am wondering if any of you out there that do image analysis of breast markers 
have a technologist select the field of view to be analyzed by the system. Our 
techs do this with no supervision by a pathologist. In many instances the 
pathologist changes the analyzed result without logging in to the system to 
check which areas were selected by the tech. This makes me just a bit nervous 
and I will be interested to see what you all are doing. Thanks and I look 
forward to your replies.

Many thanks,
Terri

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[Histonet] Fisher SlideMate printers

[Lima, Teresa via Histonet]

Has anyone had any issues with Fisher SlideMate slide printers and PrintMate 
cassettes printers? It seems like we are replacing print heads on the cassette 
printers far too often and the slide printers after only four weeks of use have 
already been replaced. I would be interested to hear if anyone else has 
experienced these problems.
Thanks,
Terri

Terri Lima
Histology Supervisor
Department of Pathology
Boston Medical Center
670 Albany Street
Boston, MA  02118

617-414-5332
teresa.l...@bmc.org

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