Re: [Histonet] Histonet Digest, Vol 216, Issue 5

[Linda Hines via Histonet]

STOP

On Sat, Nov 13, 2021, 11:00 AM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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> or, via email, send a message with subject or body 'help' to
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> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. Re: Paraffin embedding following storage in 70% alcohol
>   (John Kiernan)
>
>
>
> -- Forwarded message --
> From: John Kiernan 
> To: "histonet@lists.utsouthwestern.edu" ,
> Charles Riley 
> Cc:
> Bcc:
> Date: Fri, 12 Nov 2021 22:42:06 +
> Subject: Re: [Histonet] Paraffin embedding following storage in 70% alcohol
> Of course you are right!
> This is yet another example of an error in a procedure informally handed
> on from person to person! Always work from a book. Even a very old one will
> be OK for paraffin embedding.
> John Kiernan.
>
> https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html
> = = =
> 
> From: Charles Riley via Histonet 
> Sent: November 12, 2021 11:03 AM
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Paraffin embedding following storage in 70% alcohol
>
> I am working with a grad student on a project dealing with equine articular
> cartilage.
>
> The protocol she sent me for embedding the tissue samples goes directly
> from 70% alcohol to the embedding step in paraffin.
>
>
> Correct me if I am wrong but shouldn't the tissue be dehydrated fully and
> cleared before embedding the samples?
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[Histonet] HSV

[Blazek, Linda via Histonet]

Dear all,

Is there anyone out there that has a good HSV control block that they could 
spare?  I'm sorry but I don't have anything to trade since we only have small 
biopsy specimens.

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>

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[Histonet] Histotech job Gainesville FL

[Linda Margraf via Histonet]

Here is a message I am posting for Martha regarding a job at U of Florida. Her 
email is m...@ufl.edu if you are interested (or check 
their website).

Begin forwarded message:
From: "Campbell-Thompson, Martha" 
mailto:thom...@pathology.ufl.edu>>
Date: August 6, 2019 at 9:33:23 AM CDT
To: 
"'histonet-ow...@lists.utsouthwestern.edu'"
 
mailto:histonet-ow...@lists.utsouthwestern.edu>>
Subject: Request to post a position
Histotech opening available at the University of Florida Molecular Pathology 
Core- please see position #511862. Full time- 8 am- 5:00 pm with no call or 
weekends. Closed all major holidays and generous benefits package including 
sick and vacation leave. Previous histology (research or clinical) experience 
required.


Martha Campbell-Thompson, DVM PhD| Professor
University of Florida | Pathology, Immunology, and Lab. Medicine
College of Medicine | Diabetes Institute | JDRF nPOD | Director, Molecular 
Pathology Core
College of Engineering |Biomedical Engineering
PO Box 100275|1395 Center Drive, Gainesville, FL 32610|Phone: 
352.273.6129|Email: m...@ufl.edu |


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[Histonet] FW: Job posting

[Linda Margraf via Histonet]

Here is a message I am posting for Patricia.

Full-time Research Histotechnologist and Laboratory Manager
A position is available for a histotechnologist for a Research 
Pathology Core Lab in the GWU School of Medicine and Health Sciences in 
Washington, DC. This person will be expected to maintain a histology 
laboratory, including ordering of supplies, washing glassware and maintaining 
equipment for optimal function, daily logs with inventory of specimens and 
blocks, labeling of samples and slides, tissue processing and embedding, 
microtome and cryostat sections, routine stains, special stains and 
immunostains with appropriate controls, as required to meet the needs of 
researchers at GWU. This person will need to work independently to deliver 
excellent quality results in a timely manner. The person should have strong 
communication skills and managerial skills, since there will be a need to work 
with researchers in several labs and to maintain inventory from several 
sources, to keep track of the flow of specimens and charges. The position will 
require data entry into an audit-trail database and the maintenance of 
meticulous records. This person should have some experience in laboratory 
research, implementation of new techniques and the ability to trouble-shoot 
problems that might arise in this setting.
Entry level qualifications:
•  Bachelor’s degree in a clinical science with a course in Histology or 
equivalent combination of training and experience.
•  Experience working in a research setting.
•  Eligible (with certification pending) or certified as a 
Histotechnologist or Histotechnician by the American Society for Clinical 
Pathologists.

Please visit our website – 
https://smhs.gwu.edu/pcl/
Apply at GWUjobs or feel free to call me to find out more about this job 
opportunity.
Patricia Latham (202-994-5057), Research Pathology Core Lab Director

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[Histonet] CMV

[Blazek, Linda via Histonet]

Does anyone have a good CMV control block they would be willing to share?
Thanks

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>



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[Histonet] job opening

[Blazek, Linda via Histonet]

We have a Histotech position open in a GI lab located outside Dayton Ohio. This 
position is Monday - Friday day shift position.  We are a growing company that 
has just opened a brand new facility, with windows so you can actually see 
outside!  You must be HT (ASCP) certified or eligible and have a minimum of one 
year of experience as a Histology Technician. We work hard to encourage team 
values, open honest communication and a system of continuous quality 
improvement.
For consideration for this position, please send a cover letter and your resume 
directly to me at 
lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>



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Re: [Histonet] Cameras in the Laboratory

[Blazek, Linda via Histonet]

If you could share the responses you get I'd appreciate it.  
We just built a new facility.  There are cameras everywhere.  They are in the 
lab and I hate them but you soon forget that they are there.  They cover almost 
all of the lab.  There is a small area that isn't covered and we call it the 
personal scratching area.  LOL .  My office does not have a camera in it.  
Linda

-Original Message-
From: Pratt, Caroline via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, January 22, 2019 3:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cameras in the Laboratory

If anyone is using cameras in the laboratory.  Can you share your experience 
with me and the placement/use/effectiveness, etc?  
caroline.pr...@uphs.upenn.edu<mailto:caroline.pr...@uphs.upenn.edu>.  Thank you!


Caroline M. Pratt, MBA
Practice Administrator Dermpath
3020 Market Street, Ste 201
Philadelphia, PA  19104
Phone 215-349-8178
Cell 610-800-1381
Fax 215-662-6150

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[Histonet] FW: Histonet submission

[Linda Margraf via Histonet]

Here is a message I am posting for Christine/ Chrissy

From: Altemus, Christine [mailto:christine.alte...@ascension.org]
Sent: Thursday, January 17, 2019 12:02 PM
To: Linda Margraf 
Subject: Histonet submission


Hello -



We currently use the following Pulmo Panel stains from Biocare: Desmoglein 3 + 
Napsin A, TTF-1 + CK5 and p63 + TRIM29 cocktails. We have plenty of lung 
adenocarcinoma control tissue but our pathologist is requesting squamous lung 
control. If you can help, please let me know - thank you for the response. 
Chrissy @ St. Mary's Healthcare - Amsterdam, NY



christine.alte...@ascension.org>




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Re: [Histonet] Eosin on processor for biopsies

[Blazek, Linda via Histonet]

We have put eosin in the last alcohol on the processor for years.  It stays in 
the esophageal biopsies just fine.  
Linda


-Original Message-
From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, January 10, 2019 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin on processor for biopsies

Gareth,

reguarding the eosin biopsy post on the histo net. Things you might want to 
considered looking at, I cannot say for sure because I don't know your 
processing solutions/schedule:

Most Eosins are alcohol soluable, by putting the eosin in your formalin most of 
it will wash out during processing. Many labs put the Eosin in the last alcohol.

Hematoxylin is typically water soluable, if you put it in formailin processing 
usually removes water if the formailin is followed by alcohols.

I hope this helps,

Cassie


From: Gareth Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 10 January 2019 9:15 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Opinion on dye on biopsies

So, I work in a small GI lab, and I put Eosin in my first formalin on my 
processor.  My biopsies are very small and this helps, somewhat, to see the 
specimens for embedding and cutting.  But, unfortunately, the esophagus tissues 
do not absorb the eosin much.  Anyway, the hospital lab I work, part-time, in 
has started using hematoxylin to help see their biopsies.  I happen to embed 
there and I think it just makes a big mess and the tissue does not absorb much 
of the stain.
What are other labs doing to aid in making their small biopsies easier to see?  
What are pros and cons to doing this, in your opinion?
Thanks!

--
*Ms. Gareth B. Davis*, B.S., HT, QIHC  (ASCP)cm Yuma Gastroenterology Yuma, AZ 
85364
928-248-5259
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[Histonet] FW: Pulmo Panel (+) Squamous Cell Lung ctrl

[Linda Margraf via Histonet]

Here is a message I am posting for Chrissy…..
Begin forwarded message:
From: "Altemus, Christine" < >
Date: January 7, 2019 at 11:29:23 AM CST
To: "lindamarg...@gmail.com" 
mailto:lindamarg...@gmail.com>>
Subject: Pulmo Panel (+) Squamous Cell Lung ctrl

Hell o -



Can you please post to Histonet website for me?



 I am looking for (+) Squamous cell lung cancer control for the Pulmo Panel IHC 
Stains. Thank you!



Chrissy

St. Mary's Healthcare

Amsterdam, NY



christine.alte...@ascension.org


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[Histonet] Histotech needed for Greenville TX lab

[Linda Margraf via Histonet]

Here is a job opening in Greenville, TX I was asked to put on the Histonet 
list..

Pathology Associates of Greenville Texas is seeking to hire a Full-time 
Histotechnician for a  4 Pathologists CAP accredited Laboratory. We are a fully 
automated Histology Laboratory  The successful candidate should be familiar 
with: Embedding, Microtomy, Leica H& E stainer, Leica Bond Immunohistochemistry 
and be able to perform Special Stains. Understands administrative duties of the 
Histology Laboratory. Willing to learn additional Molecular related assays 
specifically performed and help with Frozen sections . Process  small routine 
biopsies.
We have an excellent benefit package .
Salary: $35,000.00 to $41,000.00 /year, depending on experience .
Interested; contact mebra...@pathologyassociatestx.com and 
cw...@pathologyassociatestx.com

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[Histonet] Bouin's Fixative Substitute

[Linda Miller via Histonet]

New! Bouin's Fixative Substitute is a low-hazard replacement for Bouin's
Fixative.  This substitute provides all the benefits of Bouin's without the
use of picric acid. Available in a variety of sizes and pre-filled specimen
containers.

Available at IMEB, Inc.


-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Friday, October 19, 2018 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 179, Issue 16

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

   1. toluene substitute (Michelle Jamison)
   2. RUO antibody (Erin McCarthy)
   3. Re: Restaining old Heamatology smears (Eddie Martin)
   4. Re: BMP-2 protocol for bone (Eddie Martin)


--

Message: 1
Date: Thu, 18 Oct 2018 14:48:50 -0600
From: Michelle Jamison 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] toluene substitute
Message-ID: 
Content-Type: text/plain; charset=utf-8; format=flowed

Hi All, I am from industry. We are interested in developing an instrument
that stains then prepares microscope slides to be coverslipped. If you do
coverslip, what substitute do you use instead of toluene to go to mounting
media? Thank you! Michelle
-- 

All the best to you,

Michelle Jamison

/Bio Research Associate /

/ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA

m.jami...@elitechgroup.com  ? 
_www.elitechgroup.com  _

Tel : +1.435.752.6011 Ext: 1474

Logo


--

Message: 2
Date: Thu, 18 Oct 2018 16:30:14 -0500
From: Erin McCarthy 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RUO antibody
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Hi All,

I am looking to see if anyone has had any experience with the antibody AXL.
We are trying to work it up on our Roche Discovery Ultra and it does not
seem like it stains both nuclear and cytoplasic cell features. We only seem
to see cytoplasmic staining, and in general most tumors are showing negative
results.

I currently am using the Ultraview kit for optimization, our pathologist
likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution.

Anyone have any suggestions that might help? If you want more detailed info
I can provide as well!

-- 

Erin McCarthy, HT (ASCP)
Pathology Lab Supervisor

Tempus Labs
600 W. Chicago Ave.
Chicago IL 60654
Ph:(312) 638-6344 Ext.3835

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privileges or rights of confidentiality.? If you are not the recipient, or
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Message: 3
Date: Thu, 18 Oct 2018 18:20:53 -0400
From: Eddie Martin 
To: "Reilly, Laurie" 
Cc: "Histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Restaining old Heamatology smears
Message-ID: <0e7f124e-9821-4a32-a438-63f45ad45...@gmail.com>
Content-Type: text/plain;   charset=utf-8

Hi Laurie,

Blood is considered a tissue so it is very much still Histo related. 

Regarding your smears, this also occurs in our hematopathology laboratory
from time to time when we are scanning old slides and there?s a bubble under
the coverslip that won?t let us take images with our digital scope. 

It may very well be that there is still a resinous permount film on your
slides from the old coverslip. This may simply be removed by leaving the
smear in xylene some additional time to remove the resin. Then place slides
in 100% alcohol to remove xylene residues. The following steps would be
rehydrating the slides with dilute changes in in alcohol...ie: 95%,then 85%,
then 70%, then in a few changes of distilled water. If you can?t get the pH
of your water to pH 7.2, then you 

[Histonet] FW: Leica Bond Max artifact

[Linda Margraf via Histonet]

Here is a message I am posting for Dr. Rosen:
Hello,

I am a longtime admirer of this list, and I am sure someone on here can help us 
out

We have an artifact on our immunohistochemistry (IHC) slides.

I will attempt to attach pictures using the upload tool. In case that doesn’t 
work, I am attaching a google drive link to some jpgs. The artifact is usually 
golden brown to red/pink in color, is clustered, appears both on top of the 
tissue and around it. If we do an aggressive rundown, it appears less red/brown 
and bluer.

We are using a Leica Bond MAX IHC system which is about a year old. We usually 
do two runs a day and 95% of the time we only use a red detection kit. We only 
see this on our red detection kit on our Leica bond. The brown is unaffected. 
The corresponding H slides are fine.
We started having this artifact after a month or two of production. 85% of the 
time we have some sort of artifact. Sometimes its perfect, sometimes it makes 
the tissue unreadable. We haven’t figured out any precipitating factors.

We have tried a number of things, but we still can’t get control of this.
Here is a brief overview of our protocols:
- Processor: Leica ASP300s tissue processor.
-Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) 
melting point 57c – we tried switching, and it didn’t help.
- We only use distilled water in our baths
- Mercedes Starfrost charged slides
- Oven for 30 min at 62c
- We always use a cold red detection kit
-Sometimes our runs are delayed but not more than 4 hours
- Rundown is done in our stainer (standardized), and slides are coverslipped 
with tape.
- The bulk fluid containers in the bond are cleaned weekly and refrigerated 
between runs.
- The probe is cleaned every 300 slides.
- Mixing wells are cleaned out every two weeks and replaced often.
-Covertiles are well tended to.

We have tried to correct this problem, but we can't seem to fix it.
Any ideas?

https://drive.google.com/open?id=1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA


Jason R. Rosen, D.O.
Dermatopathologist
Premier Dermatology Partners
4675 Linton Boulevard, Suite 203
Delray Beach, FL 33445
(p) 561-499-5341
(f) 561-499-5343
(e) jro...@totalderms.com


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[Histonet] Job opening Dayton Oh

[Blazek, Linda via Histonet]

We're looking for a stand out Histology Technician.  This position is Monday - 
Friday day shift position.  We are a growing company with a brand new facility. 
To be successful in this job you must be focused, methodical, precise and able 
to work collaboratively with a variety of groups.  You must be Certified or 
eligible for Board of Certification (BOC) by the American Society of Clinical 
Pathologists (ASCP) and have a minimum of one year of experience as a Histology 
Technician with competency in the areas of embedding, microtomy and special 
stains and with a basic knowledge of immunohistochemistry. You must be able to 
work as a team that values open and honest communication, embraces challenge, 
innovation and continuous quality improvement, works hard, has fun and displays 
a can-do attitude.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>


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[Histonet] job opening

[Blazek, Linda via Histonet]

We have an opening for a Histology Technician. This position is Monday - Friday 
day shift position.  We are a growing company that has just opened a brand new 
facility, with windows!  You must be Certified or eligible for Board of 
Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and 
have a minimum of one year of experience as a Histology Technician with 
competency in the areas of processing, embedding, microtomy and with a basic 
knowledge of immunohistochemistry.  We only do a minimum number of special 
stains.  We work hard to encourage team values, open honest communication and a 
system of continuous quality improvement.
For consideration for this position, please send a cover letter and your resume 
to 
dsiemploym...@digestivespecialists.com<mailto:dsiemploym...@digestivespecialists.com>
 and place your name on the subject line of the email, or contact me directly.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>


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[Histonet] job opening

[Blazek, Linda via Histonet]

We're looking for a Histology Technician, who will prepare histology slides 
from tissue sections for microscopic examination by staff pathologists.  This 
position is Monday - Friday day shift position.  We are a growing company that 
has just opened a brand new facility, with windows!  You must be Certified or 
eligible for Board of Certification (BOC) by the American Society of Clinical 
Pathologists (ASCP) and have a minimum of one year of experience as a Histology 
Technician with competency in the areas of processing, embedding, microtomy and 
a minimum number of special stains and with a basic knowledge of 
immunohistochemistry.  We work hard to encourage team values, open honest 
communication and a system of continuous quality improvement.
For consideration for this position, please send a cover letter and your resume 
to 
dsiemploym...@digestivespecialists.com<mailto:dsiemploym...@digestivespecialists.com>
 and place your name on the subject line of the email.


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>


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[Histonet] Tanner cryostat

[Linda Margraf via Histonet]

Here is a message I am posting for Bethany.

Does anyone know anything about these cryostats imported from Germany? It's the 
Tanner TN50 cryostat. I'd like to hear from someone who has worked on one. We 
are looking to purchase a new cryostat. I love the QS12 but the Tanner is 
priced more reasonable. I can't find any reviews on this cryostat.

Thanks!
Bethany
b...@derminstitutemd.com

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Re: [Histonet] Quality Measure for Pathology

[Blazek, Linda via Histonet]

Gudrun,
Now you're making me think!  I do have a cut-off.  It's at 95%.  I do keep 
records of the percentages of specimens that do not fall into the range.  But 
as to the process of solving the problem it's just counseling.  As we have only 
hit that cut-off once in the past three years I really haven't thought it out 
farther than that.  

Linda

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, June 13, 2018 11:55 AM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Quality Measure for Pathology

Linda,
that's exactly what we do here.
I wondered, if you fixed a cut-off. Because in quality-audits they often
asked the "what do you if.."-questions. 
for example: "There is an increase in the number of discrepancies in this
special month. The rate is over your defined aim. What did you do to fix the
problem? Do you keep records about the process of solving the problem?"

I was just curious, if you go further than the counting of events. 
In daily work we often know the causes of mistakes, and very often we know,
that a certain amount of mistakes can't be avoided, although everyone tries
to give his/her best. 
I think QM should help to discover the special events or circumstances, that
raise the number of mistakes above the "normal" level. I was curious, if you
defined this "normal" level for your lab.

Kind regards
Gudrun


-Ursprüngliche Nachricht-
Von: Blazek, Linda [mailto:lbla...@digestivespecialists.com] 
Gesendet: Mittwoch, 13. Juni 2018 14:38
An: Gudrun Lang
Cc: histonet@lists.utsouthwestern.edu
Betreff: RE: [Histonet] Quality Measure for Pathology

Gudrun,
Our lab is 95% biopsy specimens.  Therefor the number of pieces counted at
gross is written on the side of the cassette so that at the time of
embedding the tech knows what should be present.  That helps in being sure
there isn't a piece stuck to the lid or forceps, or popped out when opening
the cassette.  It's really more of a Quality Assurance measure than tracking
a discrepancy.  I do watch how frequent there is a discrepancy though and if
there seems to be a pattern will initiate a review of processes for both the
grossing person and the embedding person.  
What we see most is the gross will say one piece and there will actually be
multiple tiny pieces or two pieces will be three at embedding.  As all staff
is aware that we are following this they tend to be much more precise in
counting at grossing and embedding.  
Linda

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, June 13, 2018 4:19 AM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Quality Measure for Pathology

Linda,
How do you handle this key figure of discrepancies? Have you set a cut-off
(per person, per day) for any consequencies? What are the
consequencies, besides repeated training?

thanks
Gudrun

-Ursprüngliche Nachricht-
Von: Blazek, Linda via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Dienstag, 12. Juni 2018 18:55
An: Normington Lacy; Amy Self
Cc: histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Quality Measure for Pathology

Also a discrepancy between the number of pieces that are dictated at gross
as being in a cassette compared to what is found in the
cassette at embedding.  You can record the number of blocks that need
re-embedded and the number of slide recuts needed a month. 

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Normington Lacy via Histonet
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 9:34 AM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Quality Measure for Pathology

Some ideas:

Proficiency Testing
Corrected Results (Addendums/Amendment) volume
Turnaround Time Monitoring
Frozen Section TAT (critical call)
Rejected Tests (empty bottles, etc)


-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 8:18 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Quality Measure for Pathology

WARNING: This email appears to have originated outside of the UW Health
email system.
DO NOT CLICK on links or attachments unless you recognize the sender and
know the content is safe.




Good Morning,

I am looking for ideas/suggestions for QM Histology/Pathology.  My QM
director wants me to "measure" something that I can place on
the lab dashboard.

Thanks in advance for your help..

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through better health.
NOTE:
 The information contained in this message 

Re: [Histonet] Quality Measure for Pathology

[Blazek, Linda via Histonet]

Gudrun,
Our lab is 95% biopsy specimens.  Therefor the number of pieces counted at 
gross is written on the side of the cassette so that at the time of embedding 
the tech knows what should be present.  That helps in being sure there isn't a 
piece stuck to the lid or forceps, or popped out when opening the cassette.  
It's really more of a Quality Assurance measure than tracking a discrepancy.  I 
do watch how frequent there is a discrepancy though and if there seems to be a 
pattern will initiate a review of processes for both the grossing person and 
the embedding person.  
What we see most is the gross will say one piece and there will actually be 
multiple tiny pieces or two pieces will be three at embedding.  As all staff is 
aware that we are following this they tend to be much more precise in counting 
at grossing and embedding.  
Linda

-Original Message-
From: Gudrun Lang [mailto:gu.l...@gmx.at] 
Sent: Wednesday, June 13, 2018 4:19 AM
To: Blazek, Linda
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] Quality Measure for Pathology

Linda,
How do you handle this key figure of discrepancies? Have you set a cut-off (per 
person, per day) for any consequencies? What are the
consequencies, besides repeated training?

thanks
Gudrun

-Ursprüngliche Nachricht-
Von: Blazek, Linda via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Dienstag, 12. Juni 2018 18:55
An: Normington Lacy; Amy Self
Cc: histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Quality Measure for Pathology

Also a discrepancy between the number of pieces that are dictated at gross as 
being in a cassette compared to what is found in the
cassette at embedding.  You can record the number of blocks that need 
re-embedded and the number of slide recuts needed a month. 

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Normington Lacy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 9:34 AM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Quality Measure for Pathology

Some ideas:

Proficiency Testing
Corrected Results (Addendums/Amendment) volume
Turnaround Time Monitoring
Frozen Section TAT (critical call)
Rejected Tests (empty bottles, etc)


-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 8:18 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Quality Measure for Pathology

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




Good Morning,

I am looking for ideas/suggestions for QM Histology/Pathology.  My QM director 
wants me to "measure" something that I can place on
the lab dashboard.

Thanks in advance for your help..

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through better health.
NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this
message is not the intended recipient, or an employee or agent responsible for 
delivering this message to the intended recipient,
you are hereby notified that any dissemination, distribution or copying of this 
communication is strictly prohibited. If you have
received this communication in error, please notify us immediately by replying 
to this message and deleting it from your computer.
Thank you.
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Re: [Histonet] QM Dashboard

[Blazek, Linda via Histonet]

Terri,
I completely agree with you on the recut issue but I separate the recuts by 
Quality Issue and just a deeper section.  If a section is folded, has chatter 
or some other quality issue then I think it needs addressed as a Quality 
Management issue.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 2:21 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] QM Dashboard

We use: 
1.  Report TAT
2.  Discrepant pathology reports
a.  Internal vs External report correlation
b.  Frozen section vs Final diagnosis correlation
c.  Amended reports
I liked the idea of piece count discrepancy or even poorly processed blocks.  I 
never recommend using "recuts" as a QM because that is more often a product of 
the pathologists' preference or desire to see more tissue and does not reflect 
the quality of the sections (unless it is an incomplete facing issue).  This 
can quickly become punitive for your department because this information is not 
presented to people who understand pathology.
My 2 cents. Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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Re: [Histonet] Quality Measure for Pathology

[Blazek, Linda via Histonet]

Also a discrepancy between the number of pieces that are dictated at gross as 
being in a cassette compared to what is found in the cassette at embedding.  
You can record the number of blocks that need re-embedded and the number of 
slide recuts needed a month. 

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Normington Lacy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 9:34 AM
To: Amy Self; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Quality Measure for Pathology

Some ideas:

Proficiency Testing
Corrected Results (Addendums/Amendment) volume
Turnaround Time Monitoring
Frozen Section TAT (critical call)
Rejected Tests (empty bottles, etc)


-Original Message-
From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 12, 2018 8:18 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Quality Measure for Pathology

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.




Good Morning,

I am looking for ideas/suggestions for QM Histology/Pathology.  My QM director 
wants me to "measure" something that I can place on the lab dashboard.

Thanks in advance for your help..

Amy Self
Histology Lab Senior Tech
Lab
Tidelands Georgetown Memorial Hospital
606 Black River Road
Georgetown, SC 29440
843-520-8711
as...@tidelandshealth.org
Our mission:  We help people live better lives through better health.
NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this message is not the intended 
recipient, or an employee or agent responsible for delivering this message to 
the intended recipient, you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. If you 
have received this communication in error, please notify us immediately by 
replying to this message and deleting it from your computer.
Thank you.
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[Histonet] Jobs in Wilmette Illinois

[Linda Margraf via Histonet]

Amber contacted me asking to post these jobs for a histotech and lab manager on 
the list. She is the Director of Clinical Operations
Illinois Center for Digestive and Liver Health.  Please contact her by email if 
you have questions or are interested.
am...@icdlh.com
Thanks
Linda
Histonet administrator


Job Description
Gastroenterology practice is requesting a
Histotechnologist for the Wilmette pathology laboratory
Minimum experience needed is 2 years as a histotechnologist.
Competitive hourly compensation.
HTL (ASCP) certification required .
Baccalaureate degree from a regionally accredited college/university
Duties
-Perform routine histology activities involved in preparation of tissue biopsy 
specimens for -Histological processing according to policies and procedures
-Assure appropriate specimen accessioning and labeling
-Assist with clerical functions associated with specimen preparation when 
necessary
-Perform gross examination and description of tissue
-Order stains per established criteria
-Operate microwave tissue processor
-Embed biopsy specimens
It is a part time position
Job Description
We are looking for a part-time Lab Manager who can direct the daily operation 
of our pathology lab to ensure the precision of laboratory test results in 
accordance with accreditation standards and guidelines.
*   Directs the daily operations - including staff, equipment, space and 
supplies.
*   Maintains the departmental quality assurance program to ensure regulatory 
compliance and test quality.
*   Collaborates with medical director, and faculty in the development of new 
procedures or programs to enhance laboratory efficiency.
*   Adheres to regulatory, institutional and departmental policies (CAP)
*   Demonstrates quality customer service through positive interaction which 
meets the needs of co-workers, faculty, visitors and vendors.
*   Performing other duties as assigned by the medical director to support the 
department mission of service to patients and providing quality diagnostic 
information.
Job Type: Part-time


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[Histonet] Regarding too many emails

[Linda Margraf via Histonet]

Greetings Histonetters:

As the Histonet administrator, I typically don't send out too many messages to 
the list, but it is probably time for me to remind folks about some of the 
Histonet list's functionality. First, thanks to everyone for participating. We 
have over 3100 members from all over the globe which is quite astonishing for a 
list that started 20 years ago.  Thanks also to everyone who sends out 
subscribing/unsubscribing instructions etc. and for Marvin Hanna for running 
the archive all these years.  I never would have dreamed the list would still 
be running strong this long.   

It is best if you manage your own account on the membership list at the url 
below (sorry the url is so long now!).
To subscribe or unsubscribe via the World Wide Web, visit

https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=fHUKEfYp8ZKZp-Z4zyr9bXWWb-JCctOum5ZlzbjkVjM=1o7iZWfSCXEgDesmDMuV2rPUZJJnqv0nrvyFk2x0v0A=AcwNeKf6Os__vxNkSxyldTqmODmwViFPmsquElvZ7ew=bngU9RZfSDBzzUiM4ycqhQEEauD-rCoFokTODEYqYME=

Remember, if your email address changes even a little, the server won't 
recognize you as a list member when you try to post a message (but you likely 
still get Histonet messages internally routed through your email server).  If 
your email address differs from the one on the membership list, you will 
receive a non-member alert that your message won't be posted. Also, if you have 
graphics in your message such as a signature line with a logo, the server may 
reject a message you try to post to the list.   Please send the message as 
plain text (without the footer etc) if you don't see your message come through 
the list. 

Finally, for those who don't like the many emails, Histonet has a digest 
version where all the messages are grouped into one message every day.  It is a 
little confusing reading the messages as they are not grouped by topic but it 
really cuts done on the number of messages you get. If you want to switch to 
digest, go to the Histonet url above and modify your subscription. Also, you 
can hold mail while you are away, change your password  etc at that url 
address. 

Always feel free to contact me if you have issues by email at 
histonet-ow...@lists.utsouthwestern.edu
Sometimes it takes a day or two for me to get back to you but I try my best.

Thanks for participating in Histonet.
Sincerely,
Linda M



Today's Topics:

   1. please unsubscribe me. Too many emails. (Wirsbinski, Stephanie)
   4.  please unsubscribe me. Too many emails. (Kharzai, Sadeq)
   

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--

End of Histonet Digest, Vol 173, Issue 3

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Re: [Histonet] Slide printer and cassette label

[Blazek, Linda via Histonet]

Karen,

We print an average of 150 blocks a day and 250+ slides a day.  We have 2 
cassette printers and 1 slide printer.  We have had them for several years now. 
 They are Primera printers purchased from Creative Waste Solutions. 
https://cwsincorp.com/
We have had excellent results with them both in functionality and service.  
Also they can connect to your LIS if you want very easily without needing an 
expensive interface.

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, March 14, 2018 8:50 AM
To: Histonet (E-mail)
Subject: [Histonet] Slide printer and cassette label

Good Morning,
I am looking for suggestions for a slide printer and cassette labeler for a 
smaller lab.  Any suggestions.   I need it with the two patient identifier.

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you

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Re: [Histonet] Slide labels or slide labeler

[Blazek, Linda via Histonet]

I've used the Primera slide printer since it came out.  I like it a lot.  It 
needs it's maintenance kept up like anything else but other than cleaning the 
rollers there is very little else that needs cleaning.  

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Victor via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, March 07, 2018 5:59 PM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide labels or slide labeler

James,

I totally agree with your assessment of the Slidemate printers. Have not had 
any experience with the newer model. 
Applying slide labels shouldn’t be an issue when dealing with one block and 
slide(s) at a time.  Some of this could depend upon your software available to 
you. Our system generated just the labels needed for the block you were 
cutting, on demand. You should be able to get a nice Zebra printer for under 
$700.

Another of our labs used the new Sakura/Premera printer and really like it.

Victor

Sent from Mail for Windows 10

From: Vickroy, James via Histonet
Sent: Wednesday, March 7, 2018 2:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide labels or slide labeler

We have used the older version Slidemate slide labelers from Thermofisher for 
the last three years.   I think putting the patient information directly on the 
slide is preferred however the wear and tear on slide printers means higher 
maintenance costs and more down time.  Thermofisher has a newer version of the 
Slidemate now that has been designed so that there is less wear and tear on the 
machines.   Currently when one of the old labelers breaks down we have to send 
the instrument out and the cost is generally 1000 - 1500 for repair each time.  
  It also seems that the older machines have to be sent in at least every other 
year.  Our older machines have been sent in almost yearly.

We are considering switching to a slide label making system by General Data.   
One of the downsides is that we have to apply the label to each slide so 
therefore we introduce another step where the slides could be mislabeled.   The 
upside is that equipment costs are much less.   A new thermal label printer 
runs around 700.00 where as the instrument that imprints the slide label on the 
slide runs between 12000 - 15000 per unit.  We need three units.
Thermo also offers a lease system.What I need to know is what has been the 
experience with the new Slidemate AS printers?   What kind of maintenance 
issues have you encountered? Finally please let me know your latest experiences 
with both slide label printers versus slide labeling printers?

Thanks for your input.

Jim


Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com>



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
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Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

[Blazek, Linda via Histonet]

I really take exception to those finding fault with this book!  Maybe another 
word other than hack would have made some people feel better but we all know 
the intent.  Stating that "many of the fixes or secrets described are because 
some hack failed to do her/his job at an earlier step in the process" is 
totally incorrect.  Very few of the "hacks" has anything to do with someone 
failing to do their job.  I think this books intent was to help new people in 
the field of histology.  It has been a great form of dialog between our new 
tech and the older techs.  It has served a very good purpose.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc

-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, January 16, 2018 11:11 AM
To: Histonet
Subject: Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

Kind of in that same vein, but not to denigrate the book ( I have not seen it), 
I try to impress on people that "enabling" a poor process via "workarounds" or 
"shortcuts" does nothing to solve the original problem - it just prolongs the 
problem and peoples frustration with the problem. A much better path is to 
figure out why people feel the need for a workaround and then solve that 
problem. In other words, if a workaround is needed, then you have a problem 
that needs solving!

So now I have people come to me  saying  "I think this thing we do is a 
workaround for another problem." That is the kind of observation you like to 
hear.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Steve McClain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 16, 2018 4:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 170, Issue 13 histology hacks

I purchased the book and applaud the effort because there is some decent 
information.  However, the term hack is a poor choice for histology and many of 
the fixes or secrets described are because some hack failed to do her/his job 
at an earlier step in the process. (Definition of hack. transitive verb. 1 a : 
to cut or sever with repeated irregular or unskillful blows. b : to cut or 
shape by or as if by crude or ruthless strokes. As a noun it is used to mean a 
mediocre performer or worker; tiresome drudge.)

Some methods, while useful in some settings, have important cons not listed, 
cons which may be counterproductive. For example using Mercurochrome or Eosin 
to mark tissue may preclude further testing with fluorescent endpoints, such as 
FISH.  Plus if you really want to use Eosin to mark the dermis, it is far 
easier to add used Eosin to one alcohol in the tissue processor. That gives a 
visible indicator of carryover, indicating need to change or rotate solutions.

Other methods seem (to me) like workarounds or Band-Aids for Labs w poor 
grossing, poor processing or poor reagents or poor technique or poor method 
choices, eg, 2.14 describes a situation where an incompetent grosser truly 
hacks or crudely cuts into unfixed tissue yielding too thick a slice. The real 
solution is to fix the tissue before slicing. Poor fixation results in poor 
processing and poor sectioning and poor staining reactions.

For another example, Cassette sponges offer few advantages, while folding lens 
paper allows the grossers to see through the paper and know all pieces are 
inside before closing the cassette lid.  The tissue does not stick to it, and 
small flakes can be scraped from the lens paper at embedding. Last during 
folding, the forceps can be cleaned at grossing and during unfolding, forceps 
may be cleaned w the paper after embedding.  Sponges also result in greater 
solution carryover.

Several colloquial naming conventions, eg, chamber saver 2.16 for 
underprocessed tissue may be memorable to some readers, yet seem  are odd to me.

This 2.16 method is an especially useful technique which may also be done to 
extend paraffin time, whenever poor sectioning due to poor processing is 
encountered at the microtome.
Variation 1 Place the block back into the proper sized mold and return to the 
heated side of the embedding center for an hour to extend processing 
(reprocessing). Then remove the old paraffin from the mold w a plastic pipette, 
 then re-embed, replacing the paraffin w new.
Variation 2 for outside blocks we routinely replace an unknown paraffin from 
another lab by melting in a mold, and 'reprocess' in our (blue ribbon) paraffin 
for 1 hr in a mold in the embedding center then re-embed.

Good first effort, yet this book could be improved by a good editor, by more 
collaborators, by illustrations, and the addition of variations or other uses 
as described abov

Re: [Histonet] Histology Hacks - the book

[Blazek, Linda via Histonet]

I agree with Terri!  We have a newly registered tech and several of us "oldies" 
and it started a great dialog on tips we have run across in the years.  The 
book has some things we had forgotten too!  We all have been enjoying reading 
and adding little comments.  Since we are a GI lab it was great to have some of 
the other hints that our new tech hasn't had much exposure to.  
Good job Michael!

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, January 12, 2018 11:06 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Histology Hacks - the book

I just wanted to share a quick review of the new book "Histology Hacks", by 
Michael Backhus.  It is the kind of book that brings together all of the little 
tidbits that a tech picks up along a lifetime of working and networking with 
others.  It is well organized and the information is presented with the pros 
and cons of each hint.  The big bonus was that by flipping through the book in 
my department, it started a conversation on techniques and experiences with 
similar tips.  Anything that gets your Histology team excitedly talking about 
their profession is well worth the relatively small investment.  It would make 
a great staff present for Lab Week.  I don't know Michael, but kudos!  Check it 
out on Amazon.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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[Histonet] FW: Call for abstracts

[Linda Margraf via Histonet]

Here is a message I am posting  from Melinda….


Begin forwarded message:
From: "Hamilton, Melinda A" 
>
Date: January 8, 2018 at 5:10:45 PM CST

The ARIZONA SOCIETY FOR HISTOTECHNOLOGY 2018.  CALL FOR ABSTRACTS. Friends and 
Colleagues, we are now accepting Abstracts Submissions for the 2018 Arizona 
Society for Histotechnology Regional Symposium that will be held June 1-3 in 
Phoenix Arizona. We are looking for Management, IHC, Special Stains, Routine 
Histology, Molecular and MOHs. Please submit your abstracts to Antonia Miller 
(alvmiller...@gmail.com) no later than March 
1,2018.




Melinda A Hamilton
Histotechnician/ MOHs/ Cryo Lab
Arizonia Society for Histotechnology President
Banner MD Anderson Cancer Center

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[Histonet] Histotech Need Chesapeake Urology

[Linda Margraf via Histonet]

Here is a message I am posting for a subscriber.  
Linda M
 Histonet administrator

---
Hi Linda,

My initial email message got rejected.  Can you please post our opening?

Mike

About Us: Chesapeake Urology is the largest urology practice in the 
Mid-Atlantic region, providing a comprehensive array of urologic services and 
treatment options to patients. As one of the most respected and progressive 
urology practices in the nation, we have over 100 providers throughout our 26 
medical offices and 17 outpatient surgical centers. Working in a team 
environment focused on patient care, our mission is to ensure every patient 
receives a superior experience.

As one of the "Top 100 Best Places to Work in Health Care" and voted a "Best 
Place to Work" by multiple organizations, we maintain our exceptional patient 
care and culture by selecting the best people in our field, promoting a healthy 
and respectful environment, and serving the community through our outreach 
programs and events. We are proud that our people make us the best and it shows 
in the superb care we deliver to each and every patient. For more information, 
please visit our website at www.chesapeakeurology.com.
We are seeking a full-time Histotechnologist for our new Pathology Lab located 
in Beltsville, MD. This position supports the pathology histology laboratory in 
preparing, processing, and staining of tissue specimens for review by the 
pathologists. The hours are Monday-Friday and can be flexible in the 
afternoon/evening.

Responsibilities:
*Responsible for accessioning, grossing, embedding, microtomy, performing H 
and papanicolau staining, and cover slipping of all surgical/cytologic specimen 
slides for microscopic analysis.
*Loading the blocks on the processor ensuring quality and timeliness of 
production.
*Modify and validate procedures and methodologies for immunohistochemistry, 
special staining and other related staining.
*Organize cases by matching slides with appropriate paperwork and/or laying 
slides out in slide folders.
*Provide maintenance or troubleshooting of equipment and automated 
instrumentation within the laboratory.
*Assists supervisor in meeting department regulatory agency requirements (CAP, 
CLIA, etc.) *Performs quality control procedures per protocol and completes 
necessary documentation.
*Maintain supplies and other inventory.
*Perform other laboratory and office duties as assigned by manager.

Requirements
*An Associate's Degree in a laboratory science or medical laboratory technology 
obtained from an accredited institution or education/training equivalent to the 
above that includes at least 60 semester hours from an accredited institution.
*HT or HTL certification required.
*Proficient embedding, sectioning and histology techniques with human tissues.


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[Histonet] counters

[Blazek, Linda via Histonet]

Good morning.  Does anyone have a picture of a curved counter top for use with 
the microtome?  I thought I had one but can't seem to find it.

Thanks!

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>


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[Histonet] FW: Histonet post

[Linda Margraf via Histonet]

Here is a message I am posting for Samantha. Please reply to her not me, thanks!
From: "Green, Samantha" 
<samantha.gr...@cmc-nh.org<mailto:samantha.gr...@cmc-nh.org>>
Date: August 28, 2017 at 3:18:32 PM CDT
To: 'Linda Margraf' <lindamarg...@gmail.com<mailto:lindamarg...@gmail.com>>
Subject: Histonet post
Hello Histoneters,

I am reaching out to the pathology world in the hopes that someone can help us 
out. Our lab is currently validating two new staining protocols:


1.   HER2 FISH for gastric tissue (stomach & distal esophagus)

2.   ALK FISH for lung tissue

We have hit a road block without validation because we have run out of patient 
tissue. We are desperately searching for:


· 10 distal esophagus / gastric tissue POSITIVE for HER2 (preferably 
stained by FISH previously)

· 5 distal esophagus / gastric tissue NEGATIVE for HER2 (preferably 
stained by FISH previously)

· 20 specimens (surgical or cytology cell block) POSITIVE for ALK 
(previously stained by FISH only)
We would need two charged slides with 5 micron sections and proof of stain 
results (example: de-identified report).

We understand this is a lot of work but anything thing would help, even if it 
is just one or two cases. We are not asking anyone to “work for free” so we are 
100% willing to trade help for tissue blocks or purchase the slides if 
necessary. We do have TONS of FFPE tissue blocks that are HSV I & II positive. 
I know that HSV can be hard to come by and we would be willing to send some 
tissue blocks in exchange for the slides listed above.

Please either post on this blog or contact me directly at 
samantha.gr...@cmc-nh.org<mailto:samantha.gr...@cmc-nh.org> .

Thanks for reading!

Best regards -

Samantha Green, B.S.  HTL (ASCP)


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[Histonet] FW: PAX-5

[Linda Margraf via Histonet]

Here is a message I am posting for Beth….


From: "O'neil, Beth" >
Date: July 21, 2017 at 2:31:24 PM CDT
To: 
"histonet-ow...@lists.utsouthwestern.edu"
 
>
Subject: PAX-5
My pathologists are not happy with PAX-5 on the Ventana Ultra; it will 
occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to 
be a nuclear stain).  We use Ventana’s clone SP34. I have been trying to 
optimize with the Optiview kit but we are getting so much background staining 
and only sporadic staining of the R-S cells.  I tried a different clone, Cell 
Marque EP156 which was really clean but would not stain the R-S cells at all, 
no matter what we did.   My Ventana technical specialist is stumped as to why 
we’re having so much trouble.  Does anyone else have the same problem or have 
any suggestions?

Beth Ann O’Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor, Technical Specialist
Lab:  304 – 293 – 6014
Office:  304 – 293 – 7629
 one...@wvumedicine.org





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[Histonet] Histotech job in Glen Burnie, MD

[Linda Margraf via Histonet]

Hi Histonetters,

Here is an ad for a job Mike asked me to post to the list. Please don't send 
inquiries directly to me. Thanks!
Linda (Histonet administratIon)

We are seeking a full-time Histotechnologist for our Pathology Lab located in 
Glen Burnie, MD. This position supports the pathology histology laboratory in 
preparing, processing, and staining of tissue specimens for review by the 
pathologists. The hours are Monday-Friday from 1:00PM- 9:00PM.

Responsibilities:

* Responsible for accessioning, grossing, embedding, microtomy, 
performing H and papanicolau staining, and cover slipping of all 
surgical/cytologic specimen slidesfor microscopic analysis.

* Loading the blocks on the processor ensuring quality and timeliness 
of production.

* Modify and validate procedures and methodologies for 
immunohistochemistry, special staining and other related staining.

* Organize cases by matching slides with appropriate paperwork and/or 
laying slides out in slide folders.

* Provide maintenance or troubleshooting of equipment and automated 
instrumentation within the laboratory.

* Assists supervisor in meeting department regulatory agency 
requirements (CAP, CLIA, etc.)

* Performs quality control procedures per protocol and completes 
necessary documentation.

* Maintain supplies and other inventory.

* Perform other laboratory and office duties as assigned by manager.

Requirements

* An Associate's Degree in a laboratory science or medical laboratory 
technology obtained from an accredited institution or education/training 
equivalent to the above that includes at least 60 semester hours from an 
accredited institution.

* HT or HTL certification required.

* Proficient embedding, sectioning and histology techniques with human 
tissues

Interested applicants should complete an application at 
http://www.chesapeakeurology.com/about/employment/apply/<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.chesapeakeurology.com_about_employment_apply_=DwMFAg=fHUKEfYp8ZKZp-Z4zyr9bXWWb-JCctOum5ZlzbjkVjM=1o7iZWfSCXEgDesmDMuV2rPUZJJnqv0nrvyFk2x0v0A=KsB9C62OJ2FKr93urrbmgUoyUWqcjQCSd8p2nzLITjM=fbm_Nk6KXS7cjjdJepAFezdU4nxxyS_ndUASKUgG__4=>.
  Thanks so much.

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[Histonet] job opportunity in NY

[Linda Margraf via Histonet]

Here is a message I am posting for Jennifer.
Please don't respond directly to me. Thanks
Linda M,  Histonet administrator

Hello!
I would like to post a position on Histonet. The position is at New York 
Presbyterian Hospital-Weill Cornell. See below.

Laboratory Technologist - Immunohistochemistry - Days
Advanced Patient Care:
Laboratory Technologists Make It Possible

Laboratory Technologist - Immunohistochemistry - Days

At NewYork-Presbyterian Hospital, Laboratory Technologists are redefining the 
limits of science and medicine. We study some of the most complex and rarely 
seen medical conditions - with unmatched energy and expertise. You can help 
Make It Possible:

We're inviting the best laboratory technologists to work side-by-side in our 
Immunohistochemistry Laboratory NYP/Weill Cornell Medical Center. Here you'll 
have an opportunity to work with nationally accredited colleagues and 
contribute to lifesaving results. You'll find real opportunities to maximize 
your time and training. You'll use the latest and finest technology and 
techniques. Our implementation of a state of the art Bond Advance/Leica IHC 
Systems provides results in less than 4 hours. Take on this important role, 
responsible for optimization and validation of new antibodies as well as 
maintaining a control library.

This position is Monday through Friday from 11:30am to 7:30pm  or  8:30am - 
4:30pm, based on departmental need.

Preferred Criteria
* Immunohistochemistry experience
* Experience cutting frozen sections for immunofluorescence studies
* Computer literacy with strong IT skills
* Experience using CoPath
* ASCP certification

Required Criteria
* A Bachelor's degree in Medical Technology or related health science 
field
* New York State Clinical Laboratory Technologist licensure from the 
New York Education department

To apply please visit: 
http://careers.nyp.org/new-york-jobs/laboratory-technologist-immunohistochemistry-days/00735216#summary<https://urldefense.proofpoint.com/v2/url?u=http-3A__careers.nyp.org_new-2Dyork-2Djobs_laboratory-2Dtechnologist-2Dimmunohistochemistry-2Ddays_00735216-23summary=DwMFaQ=fHUKEfYp8ZKZp-Z4zyr9bXWWb-JCctOum5ZlzbjkVjM=1o7iZWfSCXEgDesmDMuV2rPUZJJnqv0nrvyFk2x0v0A=G6f0_z_oM5QllZL6mQh9RO-oQanD8by1uEwQtsBXZMI=MDYSeZ9hkhuSNbSedrME-Apn_NHb5vtKRXGEdiQ2wLw=>


Thank you!

Jennifer Cianchino
Quality Supervisor
Immunopathology Department

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Re: [Histonet] cassette printers - multi color cassettes and network ready-

[Blazek, Linda via Histonet]

Cheryl,
We use the Primera printer and have had great success with it.  It's small and 
reliable.  You can get it from http://cwsincorp.com/

Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Cheryl via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 15, 2017 3:57 PM
To: Histonet
Subject: [Histonet] cassette printers - multi color cassettes and network ready-

What are you using for your cassette printer?  The Leica IP-C is a familiar 
model but it is SO BiG.
We need 3-5 colors of cassettes, and it has to network so we can have multiple 
users.  We print two lines (number and name) with hopes to go to full bar 
coding in the near future. Looking for any/all input - vendors welcome, too. 
Cheryl Kerry, HT(ASCP) ADG pathologycke...@adgpath.com 
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Re: [Histonet] looking for a slider printer

[Blazek, Linda via Histonet]

Blanca,

We have had the  Primera Slide printer for several years now. It's a great 
small footprint printer and easily connects to our LIS.  Check with Creative 
Waste Solutions.  http://cwsincorp.com/
They sell them.  
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, April 21, 2017 12:09 PM
To: 
Subject: [Histonet] looking for a slider printer

Dear Histonets!!!
We are looking to buy a slide printer for a low cost, durable and easy to work. 
W are a very small lab, we don't' need anything fancy. If you know somebody or 
have a good experience with one please let me know.


Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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Re: [Histonet] solvent recyclers

[Blazek, Linda via Histonet]

I have had the recycler from CBG for years and it's been a great workhorse!


Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, March 23, 2017 11:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] solvent recyclers

Hi everyone,

We are looking into getting a new solvent recycler to recycle our xylene and 
alcohol, any recommendations?

Thanks!



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Re: [Histonet] Bob Richmond

[Blazek, Linda via Histonet]

Happy retirement Dr. Richmond!  Just don't retire from the histonet!  We need 
you.  I too have a wonderful Samurai collection that someday I'll hand down to 
a "newbie" that will just scratch their head and say "really"
Your advice and humor have been appreciated (and collected) more than you know!
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com

-Original Message-
From: Gagnon, Eric via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, March 13, 2017 10:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bob Richmond

Happy 'retirement', Bob. Old pathologists never retire. They are just 
glassy-eyed!



Appreciative of your many years of pragmatic, might I say homespun, advice and 
counsel on this list. Many of your posts have been printed off and retained in 
my Big Binder of Histo-Truth.



Enjoy!



Eric Gagnon

Histology Laboratory

Kingston General Hospital,

Kingston, Ontario, Canada.





>The old Samurai Pathologist - now retiring at 78 - thanks the many
histotechnologists who've kept him out of hot water the past 52 years.

Too bad we can't have more kids in search of a career reading Histonet - or at 
least, the Help Desperately Needed notices!

Bob Richmond
Samurai Pathologist
Maryville TN

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Re: [Histonet] cassette printer additional request

[Blazek, Linda via Histonet]

We have the Primera Signature Cassette printer and like it a lot.  Support is 
excellent but we haven't needed it much.  Set up is easy.  Printing is very 
good.  We also have their slide printer that is interfaced with our LIS.  It's 
sold by Creative Waste Solutions.
 http://cwsincorp.com/
If you'd like any questions answered feel free to contact me.

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Eileen Akemi Allison via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, March 01, 2017 8:31 AM
To: Histonet
Subject: [Histonet] cassette printer additional request

Hi Again:

I forgot to mention I am open to investigate other Cassette Printers other than 
the General Data Printer… AlOf course, price, and dependability is a big 
factor. Would love to hear your comments, good, bad or indifferent...

Thanks in again!

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com>
H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com>

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Re: [Histonet] BRG1 IHC

[Linda Margraf via Histonet]

Richard,

Propath Labs in Dallas TX offers BRG1 IHC. I have used it a few times now on 
brain tumor cases and it seems great. We use their immunohistochemistry 
services all the time.  Propath.com is their website.


Linda M
Histonet administrator
--

Message: 12
Date: Thu, 23 Feb 2017 17:31:54 +
From: "Cartun, Richard" <richard.car...@hhchealth.org>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] BRG1 IHC Testing
Message-ID:
<9215bd4b0ba1b44d962a71c758b68d2e953f4...@hhcexchmb03.hhcsystem.org>
Content-Type: text/plain; charset="us-ascii"

Is anyone doing clinical IHC testing for BRG1/SMARCA4?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.


*

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[Histonet] cytology and histology jobs in IL

[Linda Margraf via Histonet]

Here is a message I am posting for  Katrina, a non-Histonet member. Please 
contact her (email address at the bottom)if you have questions. Thanks!



ACL Laboratories, Rosemont, IL
Supervisor Cytology Lab


ACL Laboratories is jointly operated by Wisconsin-based Aurora Health Care and 
Advocate Health Care.  Created via a merger of several established 
laboratories, ACL continues a 40-year tradition of providing a full range of 
lab services.  We are able to draw on the resources two major health care 
systems which uniquely positions us to provide superior service on a wide range 
of clinical and analytical testing, including molecular diagnostics.  We also 
offer 24-hour turnaround on most routine lab tests - including toxicology, 
environmental, and specialty tests.  Our staff includes board-certified 
pathologists, PhD chemists and microbiologists, registered medical 
technologists/technicians, cytotechnologists, histotechnicians, and specialists 
in information technology.


Highlights

* Advocate Award Winning: Advocate Healthcare is voted the "Top 100 
Best Places to Work" by the Chicago Tribune, multiple years in a row

* Advocate Serving the Community:  12 Advocate Hospitals and over 250 
sites of care.

* Advocate and ACL Market Leadership: 90 board certified pathologists 
on staff

* Employee - Centric: ACL Laboratories is the largest hospital system 
laboratory in the United States consisting of two central labs, one in 
Rosemont, IL and one in West Allis, WI.

* ACL Laboratories: Located just off I-294, the Rosemont Central 
Laboratory offers easy access by Metra, CTA (L-train and bus) and has free 
parking.

Description:
Serve as managing technologist for the Cytology clinical laboratory section. 
Incumbent will be responsible for coordinating all technical, logistical, and 
administrative functions to ensure prompt and accurate testing and reporting of 
results for clinical laboratory samples within established timeframes.
Qualifications required
* Associate or baccalaureate degree in laboratory science.
* Professional certification by one or more of the following: American 
Society of Clinical Pathologists, National Certification Agency for Medical 
Laboratory Scientists or equivalent.
* Position involves moderate and/or high complexity testing as defined 
by CLIA '88.
* Minimum five (05) years laboratory-related experience with expertise 
in anatomic pathology and cytology.
* Excellent verbal, written, and interpersonal communication skills.
* Excellent computer skills including, but not limited to word 
processing, spreadsheet, and e-mail use.
* Thorough knowledge of statistics and ability to apply statistical 
rules/analysis to laboratory decision-making processes.
* Previous supervisory experience highly desirable.

To learn more about the position, along with submitting an application for 
consideration, please click on the link below to take you to the job posting: 
https://career4.successfactors.com/sfcareer/jobreqcareer?jobId=57566=advohealth=
ACL Laboratories, Rosemont, IL
**$3000 sign on bonus**
Disbursement after 90 days, 1yr and 18 months of employment

Histotechnician:

At Advocate Health Care our Histotechnicians work closely with the clinical 
pathologist preparing specimens for diagnosis.  If you have a demonstrated 
ability to produce high quality slides in a fast paced environment this may be 
an opportunity for you!
Position: Histology Technician, Core Lab - Full-time, Part-time and various 
shifts with weekend rotation and emergency call.
Highlights:

  *   Advocate Award Winning: Advocate Healthcare is voted the "Top 100 Best 
Places to Work" by the Chicago Tribune, multiple years in a row.
  *   Advocate Serving the Community:  12 Advocate Hospitals and 77 patient 
service centers.
  *   Advocate and ACL Market Leadership: 90 board certified pathologists on 
staff.
  *   Employee - Centric: ACL Laboratories is the largest hospital system 
laboratory in the United States consisting of two central labs, one in 
Rosemont, IL and one in West Allis, WI.
  *   Clinical Laboratory Expertise: Excellence in all areas - Core lab, 
Histology, Molecular, Cytogenetics, Pathology, Cytology, Microbiology and 
Industrial Toxicology.
  *   ACL Laboratories: Offers easy access by Metra, CTA (L-train and bus) and 
has free parking.

Histotechnician Position Responsibilities include but are not limited to:

  *   Identification of specimens, frozen sections and immunoperoxidase stains 
for diagnosis.
  *   Producing high quality cryo, paraffin, histochemical and immunoperoxidase 
diagnostic slides.
  *   Providing technical assistance to the pathologist and surgeons with rapid 
diagnosis of frozen tissues sections during a surgical procedure.
  *   Performance of preventative maintenance of laboratory equipment to ensure 
optimal operational integrity.
  *   Systematic maintenance of 

Re: [Histonet] (no subject)

[Blazek, Linda via Histonet]

Try the BioCare antibody.  It's excellent.

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, December 01, 2016 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Since, Cell Marque H. Pylori is not available,  can some one give a comparable 
antibody to order?  Thanks in advance


Nirmala Srishan








Holy Name Medical Center is ranked among the top hospitals in the nation 
for patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If 
you are not the intended recipient, you are hereby notified that any 
disclosure, copying, distribution, or taking of any action in reliance on 
the content of this message is strictly prohibited. If you have received 
this communication in error, please notify the sender by replying to this 
message, and then delete it from your system.




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[Histonet] new lab

[Blazek, Linda via Histonet]

Hi all,

It's finally official.  We will be building a new facility with ground breaking 
in the spring!  I have seen the drawings for the designated area in the new 
building for the histology lab.  Now I could use any suggestions that you all 
may have in designing a new lab.

Thanks in advance!
Linda

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>


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[Histonet] rotomat

[Blazek, Linda via Histonet]

Good morning,
Does anyone have any firsthand knowledge of the Hanel Rotomat slide storage 
system?
Thanks,

Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com<mailto:lbla...@digestivespecialists.com>


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Re: [Histonet] Two week temp -- full travel-mistake

[Linda via Histonet]

Please disregard my post.  I apologize.  I didn't realize I was answering the 
community.
Thank you.

  From: Linda via Histonet <histonet@lists.utsouthwestern.edu>
 To: Cheryl <tkngfl...@yahoo.com>; Histonet <histonet@lists.utsouthwestern.edu> 
 Sent: Wednesday, August 31, 2016 3:20 PM
 Subject: Re: [Histonet] Two week temp -- full travel
   
Hi Cheryl,
I am attaching a copy of my resume for the information on the assignment.  
I look forward to discussing the assignment.
Regards,
Linda Dee, BGS, HT(ASCP)847-431-3376

      From: Cheryl via Histonet <histonet@lists.utsouthwestern.edu>
 To: Histonet <histonet@lists.utsouthwestern.edu> 
 Sent: Wednesday, August 31, 2016 2:42 PM
 Subject: [Histonet] Two week temp -- full travel
  
Hi Guys-
Fully funded travel position-- it's short but starts next week.  Two weeks in 
duration.  All expenses paid.  Early AM private lab.  Embed cut stain.
If you're interested-- shoot me a copy of your most recent resume with a phone 
number and I'll call you with the 'skinny'.  Need at least two years recent 
experience and high preference for small biopsy work. 
Love this lab-- one of my favorite places to work!!
Cheryl Cheryl Kerry, HT(ASCP) 
Full Staff Inc.  
ad...@fullstaff.org 800.756.3309 Phone & Fax

https://www.facebook.com/TheHistologyCompany/
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Re: [Histonet] Two week temp -- full travel

[Linda via Histonet]

Hi Cheryl,
I am attaching a copy of my resume for the information on the assignment.  
I look forward to discussing the assignment.
Regards,
Linda Dee, BGS, HT(ASCP)847-431-3376

  From: Cheryl via Histonet <histonet@lists.utsouthwestern.edu>
 To: Histonet <histonet@lists.utsouthwestern.edu> 
 Sent: Wednesday, August 31, 2016 2:42 PM
 Subject: [Histonet] Two week temp -- full travel
   
Hi Guys-
Fully funded travel position-- it's short but starts next week.  Two weeks in 
duration.  All expenses paid.  Early AM private lab.  Embed cut stain.
If you're interested-- shoot me a copy of your most recent resume with a phone 
number and I'll call you with the 'skinny'.  Need at least two years recent 
experience and high preference for small biopsy work. 
Love this lab-- one of my favorite places to work!!
Cheryl Cheryl Kerry, HT(ASCP) 
Full Staff Inc.  
ad...@fullstaff.org 800.756.3309 Phone & Fax

https://www.facebook.com/TheHistologyCompany/
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Re: [Histonet] I guess I've found it at last (nowakc)

[Linda Margraf via Histonet]

Histonetters,
I think this message below is SPAM of some sort and I recommend not opening the 
link. Several people have commented on it now. I have removed the person who 
sent it from the list. If that person was erroneously removed, they should let 
me know. 
I know people's email addresses get hijacked sometimes. Anyway, whenever an odd 
message like this with a link pops up, please don't open it.
Thanks
Linda M
Histonet administrator



Date: Wed, 24 Aug 2016 02:53:10 +0300
From: nowakc <now...@comcast.net>
To: "Norton Renewals"
<noreply_subscripti...@subscriptions.norton.com>,   "histonet"
<histonet@lists.utsouthwestern.edu>, "histonet-request"
<histonet-requ...@lists.utsouthwestern.edu>, "Marcie DuVernay"
<histotechl...@gmail.com>
Subject: [Histonet] I guess I've found it at last
Message-ID: <0cce342f$61b56200$f909e686$@comcast.net>
Content-Type: text/plain; charset="us-ascii"

Hey friend, 

As you know I've  been looking for some  suff for a long time, and I think I've 
 found it at  last, just take  a look 
<http://curdothyshe.lowcostcomputerstorage.com/lnrgfeuo>

Faithfully, nowakc






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[Histonet] Corey's apology

[Linda Margraf via Histonet]

Hi Histonetters,

Corey Hubbard at Rankin sent me a message earlier today to apologize for 
accidently sending a message he intended to go to one person, out to the entire 
list. He did not intend to "advertise" and wanted to see if I could retract the 
message. Unfortunately, I cannot retract messages that go to the Histonet 
server and it has already gone out to the list.  Anyway, the rules still apply 
about vendors. They are always welcome and encouraged to post to the list 
regarding subscribers questions and concerns but we cannot allow the forum to 
be used for advertising as it is run by the University of Texas and must remain 
an unbiased platform.

Thanks everyone for their continued participation on Histonet, by the way.  We 
still have>3300 subscribers which is highly unusual for a listserv that has 
been in operation for nearly 20 years!
Linda M
Histonet administrator
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[Histonet] expiration dates

[Blazek, Linda via Histonet]

Does anyone see an expiration date printed on their can of Freeze Spray?
I was told by the company I purchase mine from that it was not needed because 
it was "not a medical device".

Thanks
Linda

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[Histonet] Start up Vet/Animal histology laboratory

[Linda via Histonet]

 Hello Histoland,
I am inquiring if anyone has started their own lab to do histology services for 
veterinarians or animal research tissues?
Or anyone who has started a private lab?
What are your experiences?  What didn't you expect?  
Thank you in advance.
Regards,
Linda Dee, BGS,HT(ASCP) lmd...@yahoo.com

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[Histonet] Fwd:

[Linda Margraf via Histonet]

> From: Cindy Bulmer 
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
> 
> Hello Histoland,
>  
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>  
> 1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) 
> for 1 hr.
>  then put slides in refrigerator for future use.
> 2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and 
> put slides
>  directly in refrigerator for future use.
> 3)  Cut "fresh" every time they order the Ab.
>  
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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[Histonet] Stain problem

[Linda Margraf via Histonet]

(Here is a message I am posting for Natachaplease reply to the list so she 
sees it. Thanks Linda M, Histonet administrator)

Hi histonetters,

Lately we have had a special stain phenomenon occurring with our recent liver 
biopsies on our NexES special stainer. The nuclear staining is obsolete. Where 
the nucleus should be there are empty spaces. The liver control sections are 
staining fine with proper digestion and nuclear staining but the patient 
sections are show no nuclear staining. We have seen this in the past and 
describe it as a "nuclear bubbling" affect with the PAS/D on this stainer. 
However, the current nuclear staining is an extreme case. Any thoughts.

Our protocol is Schiff's 4 min @ 60°C and diastase for 12 min.

Natacha M.
Houston, Tx

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[Histonet] specimen submission pads

[Linda Margraf via Histonet]

Hi Histonetters,
We are having trouble in the Gross room,  removing small specimens from the 
pads/gauze that OR personnel put them on which they then immerse in formalin.  
We thought the gauze was challenging to remove tiny specimens from but have 
found that the Telfa pads the OR is now using come apart in the fixative and 
are even more challenging to work with. The GI lab uses sponges but they would 
be too small for the usual size containers the OR send us.  Any suggestions?

Thanks in advance,
Linda M
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Re: [Histonet] unsolved proplem

[Linda via Histonet]

Hello,
Did you call the vendor/manufacturer of your chemicals?  Sometimes, if they 
have to dispense the chemicals from a different place it can cause issues.  I 
had that happen with reagent alcohols.
Good luck,
Linda Dee, BGS, HT(ASCP)
 

  From: Jay Lundgren via Histonet <histonet@lists.utsouthwestern.edu>
 To: Jennifer MacDonald <jmacdon...@mtsac.edu> 
Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu>
 Sent: Thursday, January 28, 2016 12:46 PM
 Subject: Re: [Histonet] unsolved proplem
   
What kind of processor are you using?

On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> There are a couple of things that it might be.
> 1.  Uneven deparaffinization before staining.
> 2.  Water in your last reagents/paraffin on the processor.
>
>
>
> From:  mohamed abd el razik via Histonet
> <histonet@lists.utsouthwestern.edu>
> To:    "Histonet@lists.utsouthwestern.edu"
> <Histonet@lists.utsouthwestern.edu>
> Date:  01/26/2016 11:33 AM
> Subject:        [Histonet] unsolved proplem
>
>
>
> Dear allI have submitted a proplem about reprocessing tissue blocks that
> have patches of stained areas and unstained pale yellow areas (H
> stain)!!!, unfortently the proplem didn't solved yet and i have tried all
> possible solutions, increasing processing time and ordered new chemicals
> from other soruces and changed the paraplast company but still have the
> proplem 
> i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each
> for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl
> benzoat 2hs at least then paraffin1+2 (Maccormick melting degree
> 56-58)each 90 mins for mice kidney, liver and tests
> the previous protocol was very satisfactory to us and we have tried to
> stain older sections processed befor the emerging proplem and have a nice
> stain by current H stain to ensure that the proplem is out our staining
> step.
> any suggestions please !!
> Mohamed
>
>
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[Histonet] Fwd: Acetone and IHC

[Linda Margraf via Histonet]

Sent from my iPhone

Begin forwarded message:

> From: "Dyer, Terry" 
> Date: January 18, 2016 at 11:59:14 AM CST
> To: "'histonet-ow...@lists.utsouthwestern.edu'" 
> 
> Subject: Acetone and IHC
> 
> I would appreciate input in reference to cytology button preparations and IHC 
> staining.
> Our laboratory commonly uses acetone and alcohol to make cytology buttons 
> from body fluids.  We often perform IHC on these
> Cell blocks.  Does anyone use acetone for cytology preparation?  If so 
> does this cause staining issues or hinder staining of the
> Cell block?
> Thank you,
> Terry Dyer HT (ASCP)
>Torrance Memorial Medical Center
>3330 Lomita Blvd.
>Torrance, CA.  90505
> 310-325-9110 X1740
>  
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Re: [Histonet] Not work related, but one of the last

[Linda via Histonet]

 This is a professional histology information exchange. 



  From: Lester Raff MD via Histonet 
 To: "'histonet@lists.utsouthwestern.edu'"  
 Sent: Tuesday, December 29, 2015 11:20 AM
 Subject: [Histonet] Not work related, but one of the last
   
A blog post for those of you who have travel stories.

http://www.chicagonow.com/downsize-maybe/2015/12/an-open-letter-to-doug-parker-ceo-of-american-airlines/

Finally, since I have no desire to upset the membership of this list, I am 
going to fade away from posting here. Those of you who enjoy these posts, 
either let me know here, or at les.r...@post.com and 
you will be added to our subscription list. Or subscribe right from the website.

I may send one or two more reminders, but otherwise, my future contributions 
will be strictly based on 30 years of lab medicine.


Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Farewell

[Sebree Linda A via Histonet]

Good morning Histonetters,

Well, the time has come to say farewell to Histonet and all the people it 
represents.  I've appreciated the invaluable information, humor and 
encouragement that has been available here.  I hope I've been able to help out 
people now and then as well.  I've never regretted entering the field of 
histology 40 years ago.  I started out in clinical work, followed by two stints 
in research and finished by helping start the immunohistochemistry/in situ 
hybridization lab that I work in today.  I've always been able to keep the 
patients we serve in mind as I've gone about my daily work.  That's what's made 
this work so satisfying.

I leave you all with the wish that you continue to find purpose and 
satisfaction in the critical work you do.

Take care,

Linda A. Sebree, HT(ASCP)

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Re: [Histonet] Redeployment of histology equipment

[Linda Miller via Histonet]

I'm wondering if the rules / guidelines for histonet has changed for
brokers?  
(In the last month or two I have seen a couple brokers either advertising
items they sell or are looking to purchase and I had thought that was
frowned upon by Histonet.)

The posting below was the most recent, but also Rankin Biomedical had
advertised in the last couple months as they were looking for a specific
microtome (Vibratome 1000 plus, 1500 or 3000)

Thank you, 
Linda Miller
IMEB, Inc.


-Original Message-
From: John O'Brien [mailto:j...@imebinc.com] 
Sent: Friday, December 11, 2015 1:55 PM
To: li...@imebinc.com
Subject: FW: [Histonet] Redeployment of histology equipment



-Original Message-
From: Travis Herndon via Histonet [mailto:histonet@lists.utsouthwestern.edu]

Sent: Friday, December 11, 2015 12:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Redeployment of histology equipment

I am offering Tissue processors, embedding center, cryostats, microtomes,
slide stainer, thermal cycler, chemistry analyzermore. Recertified,
research, and educational pricing.
Also looking to purchase equipment.(ISO 9001 institution)

Kind Regards,

Travis Herndon
Sales Assoiciate
GMI

thern...@gmi-inc.com<mailto:thern...@gmi-inc.com>
www.gmi-inc.com<http://www.gmi-inc.com/>

Advancing Science with Affordable Solutions

ISO 9001:2008 Certified

T:763 712-8717 ext.6826

www.linkedin.com/in/travisherndon

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[Histonet] Histology equipment ads

[Linda Margraf via Histonet]

Hi Histonetters;

I have had a couple of inquiries about whether  the rules have changed 
regarding advertising on the list and the answer is no. As we run this list 
using the University of Texas software and equipment, we cannot allow 
advertising.  The line gets blurry sometimes but I had to notify the individual 
that sent the message below of the rules and he promised not to send messages 
like that again. We do still allow job postings, even from recruiters, as the 
membership seems to like receiving them.  It is fine, welcomed in fact,  for 
the vendors on the list to answer queries if they can offer assistance but they 
should not use the list to advertise their wares.
If you ever have a message you are unsure about, please contact me about 
whether it is appropriate before you post it.

Thanks,
LInda M
Histonet administrator




-Original Message-
From: Travis Herndon via Histonet [mailto:histonet@lists.utsouthwestern.edu]

Sent: Friday, December 11, 2015 12:31 PM
To: histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Redeployment of histology equipment

I am offering Tissue processors, embedding center, cryostats, microtomes,
slide stainer, thermal cycler, chemistry analyzermore. Recertified,
research, and educational pricing.
Also looking to purchase equipment.(ISO 9001 institution)

Kind Regards,

Travis Herndon
Sales Assoiciate
GMI


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Re: [Histonet] Reference lab offering ARID-1a on FFPE tissue?

[Sebree Linda A via Histonet]

Sorry, I meant ARID-1a.


From: Sebree Linda A
Sent: Friday, October 23, 2015 9:17 AM
To: Histonet (Histonet@lists.utsouthwestern.edu)
Cc: Weisman Paul
Subject: Reference lab offering ARIDIA on FFPE tissue?

Hello Histonetters,

Anyone know of a reference lab offering this antibody?

Thanks,

Linda

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

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[Histonet] Reference lab offering BRAF by IHC on FFPE specimens

[Sebree Linda A via Histonet]

We're looking for a reference lab that performs the above immunohistochemical 
stain.

Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

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[Histonet] Reference lab offering ARIDIA on FFPE tissue?

[Sebree Linda A via Histonet]

Hello Histonetters,

Anyone know of a reference lab offering this antibody?

Thanks,

Linda

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

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Re: [Histonet] Job opportunity in Colorado-

[Blazek, Linda via Histonet]

I'm sorry, I can't resist.  I guess it's just too close to vacation time...  
How strong do you have to be?

-Original Message-
From: Taylor Rinaldi via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 03, 2015 12:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Job opportunity in Colorado-

Hello all! 

 

My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We 
specialize specifically in laboratory recruiting all over the United States for 
many hospitals and reference laboratories. We just received a new order for a 
great opportunity with a well-known hospital located in Colorado. We are 
recruiting for a strong Histotechnician/Grosser for their lab. This position is 
a dayshift, fulltime, permanent opportunity. ASCP certification preferred. If 
you may be interested in this or any of our other opportunities, please reach 
out to me for immediate consideration.

 

Thank you in advance!

 

Taylor Rinaldi

Recruiting Manager

Prometheus Healthcare 

Office (301) 693-9057

  tay...@prometheushealthcare.com

 

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[Histonet] automatic stainer opinions sought

[Linda Margraf via Histonet]

We are a small lab looking for a small automatic stainer.  Has anyone used and 
have an opinion on the Sakura Histo-Tek SL automatic stainer?  
 
Connie Dieringer, HTL(ASCP)
Chief Histologist
 
Department of Diagnostic  Biomedical Science |
7500 Cambridge Street | Suite 6110 | Houston, Texas 77054 | 
713-486-4413 tel | 713-486-0415 fax
connie.j.dierin...@uth.tmc.edu



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[Histonet] Histotech position in Arizona

[Linda Margraf via Histonet]

Dear Histonetters:
Here is information (please see below)  I am posting for Rita about a position 
at the University of Arizona...Please check with her at ri...@email.arizona.edu 
if you have questions.
Thanks!
Linda M, Histonet administrator

Title: Histotechnologist 
Department: Animal  Biomedical Sciences-Res (2450) 
Location: Main Campus - University of Arizona, Tucson Arizona

Position Summary:
The School of Animal  Comparative Biomedical Sciences is currently seeking a 
well organized and highly motivated person for the position of 
Histotechnologist. The incumbent will prepare microscope slides of tissue for 
pathological studies and be responsible for all aspects of maintaining the 
histology lab. The position is focused on shrimp diseases for a diagnostic 
laboratory and shrimp research.  Other duties may be assigned as needed. The 
candidate must be highly detail oriented and able to work well on their own 
with good organization and multi-tasking skills.
Outstanding UA benefits include health, dental, and vision insurance plans; 
life insurance and disability programs; paid vacation, sick leave, and 
holidays; UA/ASU/NAU tuition reduction for the employee and qualified family 
members; state retirement plan; access to UA recreation and cultural 
activities; and more!
The University of Arizona has been recognized on Forbes 2015 list of America's 
Best Employers in the United States and has been awarded the 2015 Work-Life 
Seal of Distinction by the Alliance for Work-Life Progress! For more 
information about working at the University of Arizona, please go to this link: 
http://employment.arizona.edu/

Duties  Responsibilities:
* Maintain all aspects of the histology laboratory.
* Perform all duties according to ISO/IEC 17025 standards.
* Tissue sample preparation for histological analysis including: Dissection, 
paraffin infiltration, embedding, sectioning and staining.
* Knowledge of ISH and IHC procedures is desirable.
* Keep ISO/IEC 17025 logs of equipment maintenance, temperatures, tissue 
processing and staining.
* Maintain the laboratory's archive of histological slides, paraffin blocks and 
preserved tissues.
* Log samples into the FilemakerPro database upon arrival at the laboratory.
* Maintain FilemakerPro database and databooks: archiving of data, organization 
of databooks,
database maintenance.
* Participate as a histotech instructor during special workshops and for 
graduate students and visiting scholars.

Minimum Qualifications:
Please see Arizona Board of Regents Minimum Qualifications.
Arizona Board of Regents Minimum Qualifications
Two years of college with courses in Biology and Chemistry or eligibility for 
registration as a Histotechnologist with the American Society of Clinical 
Pathologists (ASCP) AND one year of experience sectioning and staining tissue 
and bone marrow; OR, any equivalent combination of experience, training and/or 
education.

Preferred Qualifications:
* HT or HTL ASCP certification preferred, but will consider experience in place 
of certification.
* Minimum of 1 year experience in paraffin block sectioning.
* Knowledge of the principles and practices of tissue processing and slide 
development.
* Ability to effectively communicate with others.
* Excellent organizational and multi-tasking skills.
* Knowledge of performing ISH and IHC is optional, but it would be helpful.

 Full Time 
Job Category: General 
Benefits Eligible: Yes - Full Benefits 
FLSA: Non-Exempt 
Posted Rate of Pay: $25,478 - $37,915 Annually 

Apply on-line at: https://uacareers.com/hr/postings/4542

 


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[Histonet] email issues

[Linda Margraf via Histonet]

Dear Histonetters,
I have heard from a couple of list members that they have been removed from the 
list from excess bounces and once they have gotten back on the listserv 
membership list, they no longer receive a copy of any message they post to the 
list. They use Yahoo and AOL.

I contacted the IT experts at the university who investigated. It appears Yahoo 
and probably AOL have changed settings to improve spam filtering and it is 
blocking or bouncing mail. The IT guys changed some settings on the server. The 
bouncing issue seems to be resolved but for people using those email systems, 
they may not get a copy of any messages they post to the list any longer.  
Apparently all the other mail from the list comes through just fine. If that 
happens to you, you can check the archive 
(http://www.histosearch.com/histonet.html) to see if your message is there. 
There is also a setting for your Histonet subscription, that you can turn on, 
that will send an acknowledgement when you post to the list. I am testing this 
now as I don't think we have used it before. Yahoo and AOL (or other email 
providers) customer service may also be able to help.

To change or look at your personal listserv settings on the list visit 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Remember, if you get a message about being a non-member if you try to post a 
message, it means your email address has likely changed from when you first 
subscribed and it needs updating, which you can also do at this site.
Thanks,
Linda M
Histonet administrator
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[Histonet] PLA2R on paraffin sections- IHC versus indirect immunofluorescence

[Linda Margraf via Histonet]

Here is a message I am posting for Martha...


My renal pathologists want to add this antibody and I want to see what 
methodologies everyone is using.   The article I was reading mentioned both as 
an option.I would prefer to do IHC and run it on our Bond 3, as opposed to 
the very manual indirect IF method.   Any feedback would be appreciated.

Thanks in advance for your help!

Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC
mw...@wakehealth.edumailto:mw...@wakehealth.edu

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Re: [Histonet] Advice needed on doing the technical component and professional component at two different sites

[Linda via Histonet]

Jim,
I know how you feel with confusing about not having a pathologist onsite.  I 
have lived your next adventure for over 5 years.  This is what I have done-
1.        Since the local pathologist group does not read these slides,  are 
our  grossing techs under the indirect supervision of the pathologists at the 
private company for these specimens? 
 No, you are not under indirect supervision. You are considered a reference 
lab.  Whom ever is your lab director is your supervising person.  But, if the 
director doesn't review any of same work batch, then you will need QC 
documentation from the private company.  And, your lab director will have to 
sign off after reviewing private lab documents.
2  How do you accession these specimens locally so tracking, slide preparation, 
and technical component billing can be done, without a local surgical report. 
 You are going to have to document all high complexity testing.  I have a 
tracking sheet that lists- accession #, patient's name, block#, general tissue 
type, grossing #, embed #, # of HE's sent, specials sent, who checked in  
received slides and (we receive back a slide level for our records) who sent 
back the slide.  I have a separate histology req, which is dictated straight 
into the patient's chart using dragon and a grossing template which lists our 
TC charges.  I have no LIS system.  The final report is interfaced using HL7 
windows.
3.   If you have to do a local report, who signs the gross report that just has 
a gross and maybe a statement that the slides will be read at the private 
company?
I electronically sign my gross report. Yes, I do meet the CLIA and CAP 
requirements for doing high complexity testing.  My grossing template includes 
my CLIA and CAP number and a statement mentioning all technical component was 
performed at X company with address, phone, etc. 
4.      Are there other CAP considerations I am not thinking of?  
I do not know what policies and procedures you currently have in place, so this 
question is harder to answer.  BUT you do have to have documentation of all 
shipping and tracking numbers to show CAP inspectors and in case something goes 
awry in passage of slides.
A very important point to remember is BOTH sets of charges have to match or you 
will be red flagged by insurance companies.  If the reading pathologist doesn't 
use the special stain, you can not charge.
You have to have a copy of whom ever is reading your slides credentials and 
insurance, continuing ed,etc.
You have to have ready for inspectors- person doing grossing credentials and 
documentation.
CAP is very good with answering questions.  You do have to comb through all the 
CAP regulations to see what applies and what does not.
If you are not already CAP accredited, they have a team(which you pay) who can 
check to see if you are ready for accreditation. 
Good Luck,
Linda Dee, BGS, HT(ASCP)


   From: Vickroy, James jvick...@springfieldclinic.com
 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
 Sent: Monday, July 13, 2015 4:27 PM
 Subject: [Histonet] Advice needed on doing the technical component and 
professional component at two different sites
   

Currently our dermatologists send their skin biopsies to a private lab.  Since 
we  now have our own Histology department we are proposing to do the technical 
component (grossing and preparation of slides) in house and sending the block 
and HE slides to the same private company.  They call these type of clients  
professional read only.  I understand the differences regarding billing since 
the CPT codes we are using have a professional component and a technical 
component.  My questions center around what other organizations do for 
accessioning and how they  meet CAP requirements for grossing, etc.

Normally we handle all of our other specimens in the normal way of 
accessioning, grossing (under the indirect supervision of a pathologist that 
comes to read the slides, processing, preparation of slides, and giving the 
slides to the pathologist who signs out the surgical report including the 
gross, and the rest of the surgical report.

The dermatology specimens would have to be handled differently. Our 
dermatologists want the slides read by the private company that only does 
dermatology specimens. (at least until we hire a dermatopathologist).  In the 
past the derm tissues were sent to the private company and they did all of the 
process including grossing.  Our organization has now decided that it is 
advantageous for us to do the technical portion in house and then send the 
slides to be read by the private company because of changes in billing.  The 
private company is perfectly willing to  help us make sure they get information 
they need  to diagnose the cases (they have even shown us how they like each 
type of skin biopsy sectioned and inked.  They will help us set up a system for 
them to get the slides and do the surgical reports.  Their report

Re: [Histonet] Ventana antibody - H pylori

[Sebree Linda A]

We have excellent and very clean results with the VMS H. Pylori on our Ultras.  
I can shoot you our protocol if you're interested.

Linda

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: Histology Technician [mailto:histology81...@att.net] 
Sent: Thursday, July 09, 2015 3:51 PM
To: Histonet; histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

 Does anyone else have constant problems with the Ventana antibody H pylori 
having a dirty background stain?  My pathologists are constantly complaining 
about it and when I call CS I get the same response...decon the instrument, try 
a new lot number, etc... it's never truly resolved.  Any thoughts?  Thanks!
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Re: [Histonet] Ventana antibody - H pylori

[Sebree Linda A]

Rabbit monoclonal, Richard.

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org] 
Sent: Thursday, July 09, 2015 4:06 PM
To: Histology Technician; Histonet; histonet-requ...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Histology Technician [mailto:histology81...@att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

 Does anyone else have constant problems with the Ventana antibody H pylori 
having a dirty background stain?  My pathologists are constantly complaining 
about it and when I call CS I get the same response...decon the instrument, try 
a new lot number, etc... it's never truly resolved.  Any thoughts?  Thanks!
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Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

[Blazek, Linda]

Dear Mr. Cantankerous, :)

So how would you gross a colon biopsy that was 0.1 cm and probably mucus?  

-Original Message-
From: Steve McClain [mailto:ste...@mcclainlab.com] 
Sent: Wednesday, July 08, 2015 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

My goodness 7% tissue loss is ridiculous.
What fool published that?

A 0.8% loss of tissue is beyond me.
Would any one advertise in the local paper that your center of excellence lab 
loses nearly 1% of your specimens?

possibly too small to survive processing is in my opinion, a self-fulfilling 
prophesy, one to be eschewed and denounced, or beaten out of the grosser who 
dares use that description for it is an expectation of failure.
That grosser would not last a week around me.
I would walk them to the train station and ask them not to return.

I felt less cantankerous before I read that one. 
Steve A. McClain, MD
631 361 4000


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Re: [Histonet] Ventana LCS (Liquid Cover Slip)

[Sebree Linda A]

You certainly can air/oven dry your slides instead of running them down and 
coverslipping; we do that with the slides we take off in the morning when we 
get in.  As to your problem with water/oil on your slides, something is not 
right in your dawn water rinsing, dehydration and/or clearing steps...but I'm 
sure I'm not telling you anything you don't know already.

I would change out all the graded alcohols and xylenes so you know the right 
reagents are in your containers.  Next, I would make sure that whomever is 
rinsing the slides, that they are using warm or even hot Dawn water and that 
they are rinsing long enough for your DAB slides.  If you're also running APR 
you may want to do the air/oven drying to xylene method to maintain the 
chromogen binding.  

Other than these steps, I don't know what else to suggest.  A long, long time 
ago when I worked in an old lab with water chilled pipes for air conditioning, 
I ran into a lot of humidity in the summer and actually had to add molecular 
sieve to my 100% alcohol in my dehydration set-up or my xylenes would get 
contaminated with water but we're talking the mid 70's so I'm hoping you're not 
running into something similar.

Hope this helps,

Linda

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: Burnett, Brandy [mailto:bburn...@capecodhealth.org] 
Sent: Thursday, July 02, 2015 12:07 PM
To: Sebree Linda A
Subject: RE: Ventana LCS (Liquid Cover Slip)

For IHC..We are rinsing them with dawn before running them down.

Brandy Burnett
Histotechnologist, QIHC(ASCP)
CCH Pathology/Histology
508-862-5267
bburn...@capecodhealth.org


Expert physicians. Quality hospitals. Superior care. 


-Original Message-
From: Sebree Linda A [mailto:lseb...@uwhealth.org] 
Sent: Thursday, July 02, 2015 10:17 AM
To: Burnett, Brandy
Subject: RE: Ventana LCS (Liquid Cover Slip)

Are you talking about IHC/ISH or Specials Brandy?

Linda A. Sebree
University of Wisconsin Hospital  Clinics IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-Original Message-
From: Burnett, Brandy [mailto:bburn...@capecodhealth.org]
Sent: Thursday, July 02, 2015 7:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana LCS (Liquid Cover Slip)

Anyone out there having any issues with the Ventana LCS?
When you are cover slipping are you seeing any water/residual LCS on the slides?
I was wondering if we should dry our slides before cover slipping instead of 
running them down? Any information would be greatly appreciated!

Brandy Burnett
Histotechnologist, QIHC(ASCP)
CCH Pathology/Histology
bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org


~~
This email and any files transmitted with it are confidential, and intended 
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Re: [Histonet] IHC repeat rate

[Blazek, Linda]

I would think that it would really depend on if it is a repeat due to an 
instrument problem or a human error.  I think that if a vendor says that a 5% 
repeat rate is acceptable for their instrument I would look for another vendor. 

-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Thursday, June 25, 2015 3:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC repeat rate

I once had a vendor tell me that 5% was an acceptable rate.  I am more inclined 
to use 2%

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
   
4. IHC repeat rates (Bitting, Angela K.)
Message: 4
Date: Thu, 25 Jun 2015 15:08:46 +
From: Bitting, Angela K. akbitt...@geisinger.edu
Subject: [Histonet] IHC repeat rates

What does this group feel is an acceptable repeat rate for automated IHC 
staining??
Thanks for your responses in advance.
Angie
***


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Re: [Histonet] de-waxing protocol on instruments just fact finding

[Blazek, Linda]

Rachel,

We dewax off line.  We use the same protocol that we do for any other staining; 
3 min in  3 changes of xylene (we use a xylene substitute though), 3 min in 2 
changes of 100% alcohol and one 3 min in 95%.  
We use BioCare's IntelliPath immuno stainer.  It is a completely open system.  
All of the sales reps, support techs, and service tech have always been very 
open and willing to help in any way to provide information and support for this 
stainer.  If by dewaxing you are also referring to off line antigen retrieval, 
we use the pressure cooker that BioCare has.  You can use any retrieval 
solution you choose with it.  If you need to use two different retrieval 
solutions you can fill your containers with two different retrieval reagents.  
I don't find that there is any difference in doing off line retrieval than on 
line retrieval.  
If I can answer any other questions please feel free to contact me.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com


-Original Message-
From: Rachel M Gonzalez [mailto:rac...@gbi-inc.com] 
Sent: Monday, June 22, 2015 5:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] de-waxing protocol on instruments just fact finding

Hi

Just beginning to look at instruments DAKO Ventana Leica. We may not have the 
budget but if we do However talking to the sales reps have been challenging 
as everything is proprietary.

This is for research consideration so we will use an open instrument? Which 
instrument do you like better open?

Does  manual dewaxing affect the staining? Every rep says yes... but does it?

What is the time for dewaxing when you start the instrument? What are the 
number of steps in the protocol? What is the waste generation like more or less 
than manual?

Thanks for your help.

Rachel
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Re: [Histonet] IHC turnaround time

[Sebree Linda A]

We have 2 FTEs in IHC; myself as one of two people that started the lab 20+ 
years ago and 2 histotechs that rotate through on a weekly basis.  Our monthly 
totals currently run between 2400 and 3300.  Our TAT policy is in by noon, out 
by 5pm except for dual and triple stains (need to be on an instrument by 11:00 
am) and EBER (needs to be on by ~ 9:00 am) and HER2 dual ISH (only run 
overnight).  We have 4 Roche (Ventana) Ultras that hold 30 slides each and are 
continuous access.  All instruments are used every day and at least one 
(usually 2 or 3) in use overnight.

Linda

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org] 
Sent: Friday, June 19, 2015 10:21 AM
To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC turnaround time

I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs 
who spend most of their time doing IHC, but they also help Histology embed and 
cut paraffin blocks when needed (which is most days).  For 2015 we are 
averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. 
 Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload).  
We have 5 Leica Bond Max platforms; however, we still do some IHCs on the 
bench.  We usually do 2-3 runs per day and an overnight run (except on Fridays) 
as well.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic 
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Olszewski, Dawn [mailto:dawn.olszew...@sgmc.org]
Sent: Friday, June 19, 2015 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC turnaround time

Hi Histonetters,
  6-19-15

We are wondering what the average turnaround times for IHC are for other labs.  
We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 
slides per 5 racks) with continuous feed per open rack.  All IHC orders placed 
by 12pm are usually out the same day by 5pm. We average 434 IHC slides per 
month and have a staff of 3 FTE's.

A pathologist has voiced concerns over our IHC output.  We are trying to 
determine best practices for IHC turnaround time as measured from time order 
placed to time of slide delivery.

If you could respond with your IHC TAT including number of techs and average of 
IHC slides monthly, we would appreciate any input you may be able to provide.

Thank you in advance,

Dawn Olszewski, HTL(ASCP)QIHC

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Re: [Histonet] PAS-diastase staining

[Linda Prasad (SCHN)]

We have been making our own diastase solution for years now. Works extremely 
well. The diastase is very easy to make in house. Here is the protocol for 
making your own diastase

Principle:  
The enzyme solution is applied to one of two sections of the tissue (preferably 
consecutive sections) and then both are stained by the PAS method.  The 
presence and relative amount of glycogen in the sections can be determined by 
examining the extent of loss of staining in the enzyme treated section as 
compared with the untreated section.

This amylase reagent has a long shelf life and uses an incubation time of 10 
minutes at room temperature. It is suitable for formalin and Brazil’s fixed 
paraffin sections as well as air-dried and ethanol fixed frozen sections.

Controls:   Liver containing glycogen

Reagents:   
1.  Amylase Reagent
Warning: Harmful, contains azide – see MSDS
Alpha Amylase from Bacillus Subtilis (Sigma Cat No 10070,)  1g
Oxoid PBS Tablets (Cat No BR14a)1 tablet
Distilled water 100ml
Sodium Azide0.1g
This solution, once prepared is stored at 4oC when not in use. A recycled 
antibody dropper bottle (often used in commercial immunoperoxidase kits) is 
useful for storage and application.

2.  PAS Reagents (see PAS Stain)
Warning: Suspected Carcinogen – see MSDS
 

Procedure:

1.  Dewax and hydrate paraffin sections, hydrate frozen sections.
2.  For amylase digestion, place slides on a rack, cover sections with 
amylase solution and allow to incubate for 10 minutes at room temperature.
3.  Wash slides well in water.
4.  Place slides in 1% periodic acid 10 minutes.
5.  Wash slides well in water.
6.  Rinse slides in distilled water.
7.  Place in Schiff’s reagent 10 minutes.
8.  Rinse slides in distilled water and then wash slides in tap water 3 
minutes.
9.  Counterstain slides with haematoxylin, differentiate and blue.
10. Dehydrate, clear and mount.

Results: 
•   Glycogen is extracted and so loss of PAS positive staining will occur 
in the enzyme treated section.

•   Mucopolysaccharides are not extracted and so staining will be the same 
in both sections.

Reference:   
V-M. Mangan, V. Farago, M. Kelly, and A. F. Henwood (2002)  An Amylase Reagent 
with a Long Shelf Life for the Removal of Glycogen from Tissue Sections J 
Histotechnol. 25(3): 153-4.


Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au
Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

♲  Please consider the environment before printing this email.

-Original Message-
From: Roy, Lisa [mailto:ro...@labcorp.com] 
Sent: Saturday, 30 May 2015 1:37 AM
To: Rene J Buesa; Julio Benavides; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining

I have been using the spit method for years with great results.  Depar slide to 
water, add saliva directly to slide for 10 minutes, rinse and stain PAS as 
usual.  Also American MasterTech has diastase malt that works well.
Lisa

-Original Message-
From: Rene J Buesa [mailto:rjbu...@yahoo.com] 
Sent: Friday, May 29, 2015 11:17 AM
To: Julio Benavides; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining

Sigma porcine diastase is very good.A cheaper option is very disgusting but had 
been done for decades, just spit on the section but that will carry bacteria 
and, although I have seen doing it, I have never done it.René J.  


 On Friday, May 29, 2015 10:01 AM, Julio Benavides 
j.benavi...@eae.csic.es wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine 
liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase digestion 
(I am assuming that PAS afterwards is the normal protocol for PAS) -A 
commercial source for diastase working in these samples. Is the porcine amylase 
from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


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 On Friday, May 29, 2015 10:01 AM, Julio Benavides 
j.benavi...@eae.csic.es wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine 
liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase digestion 
(I am assuming that PAS afterwards is the normal protocol for PAS) -A 
commercial source for diastase working in these samples. Is the porcine amylase 
from

[Histonet] Program in Interactive tissue microarray and quantitative digital pathology workshop announcement

[Linda Margraf]

Dear Histonetters:



Pasquale De Blasio asked me to provide you the announcement about a meeting in 
Rome this June.  I copied the content from the meeting flyer below. 
Unfortunately,  the Histonet server would block all the graphics and artwork in 
the flyer so I could not include that.   I have no personal knowledge of him or 
the meeting. His email is  
pasquale.debla...@isenet.itmailto:pasquale.debla...@isenet.it if you have 
questions about this meeting. Thanks.







PRELIMINARY PROGRAM  INTERACTIVE TISSUE MICROARRAY AND QUANTITATIVE DIGITAL 
PATHOLOGY WORKSHOP

June 8-9th 2015 University of Tor Vergata, Campus X, Rome - Italy

On-line registration: https://eventbrite.com/event/16536197211



June 8th, 2015

08:00 - 08:45 REGISTRATION

08:45 - 09:00 WELCOME Remarks - Prof. Luigi Spagnoli, Pasquale De Blasio

09:00 - 11:00 SESSION 1 - TISSUE MICROARRAY TECHNOLOGY - Chair: Pasquale De 
Blasio
- History of Tissue Microarray Technology
Ulrich F. Vogel, UKT, Institute of Pathology, Tuebingen, (Germany)
- Visualizing and Quantifying Cell Populations from Brightfield and Fluorescent 
TMA Samples
Thomas J. Diefenbach, The Ragon Institute of MGH, MIT and Harvard - Cambridge, 
MA (USA)
- Management of a Pathology TMA and Virtual Image Infrastructure for Research 
and Diagnostics
Peter Riegman, Erasmus MC Cancer Institute, Rotterdam, (The Netherlands)

- TMA Technology for: Tissue Microdissection, DNA  RNA and protein extract
Giorgio Stanta, Department of Medical Sciences, University of Trieste, (Italy)

11:00 - 11:20 Coffee break

11:20 - 13:30 SESSION 2 - PRACTICAL CONSTRUCTION OF A TISSUE ARRAY
- Organization of FFPE Archive for TMA Use in Research and Diagnostic
Luigi Spagnoli, Professor of Pathology Emeritus, University of Tor Vergata, 
Rome (Italy)
- Tissue and Cell Microarray: A Cross-Over Validation Tool for Stem Cell 
Research
Ida Biunno, Institute of Genetic and Biomedical Research (IRGB-CNR) , Milan 
(Italy)
- Concepts of Quality Assurance and Quality Control in the use of Tissues
Paolo Locatelli, Area Manager, Milestone srl, Sorisole - Bergamo (Italy)
- TMA Application in Neurodegenerative Disorders
Roberto Dominici, Analysis and Diagnostic Laboratory, Abbiategrasso Hospital, 
Milan (Italy)
- Ethical Aspects of use of TMA slides for Research and Diagnostics
Speaker to be confirmed

13:30 - 14:30 Lunch Break

14:00 - 17:00 SESSION 3 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS 
SYSTEMS
- Tissue Microarrayer Platform
Bring your own Tissue Blocks and make your TMA
- Digital Scanners  Image Analysis Software (Analyze your TMA Slide)
Bring your Tissue and TMA Slide and get them analysed
- Visit Exhibition Boots and look at instruments and tools which can enhance 
your work
Tissue Vacuum technology, Digital Scanners, Visual Imaging Software



June 9th, 2015

09:00 - 11:00 SESSION 4 - QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS

- TMA Platform for Biomarkers Validation and Clinical Applications

Pasquale De Blasio, Integrated Systems Engineering srl, Milan (Italy)

- Automated image analysis in histopathology: a valuable tool in medical 
diagnostics

George Steiner, TissueGnostics, Wien (Austria)

- Digital pathology: combining whole slide imaging, multiplex staining and 
automated image analysis

Speaker to be confirmed (Visiopharm)

- Proliferation markers and automated tumour detection

Speaker to be confirmed (Hamamatsu)



11:30 - 13:00 SESSION 4 - (Cont.) QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS

- Review of imaging solutions for integrated quantitative immunohistochemistry 
in the Pathology daily practice

Speaker to be confirmed (Leika/Aperio)

- Digital pathology: current status and future perspectives

Speaker to be confirmed (Dako)

- Virtual microscopy and digital pathology in training and education

Speaker to be confirmed (PathXL)

- Virtual Microscope System

Speaker to be confirmed (Olympus)

13:00 - 14:00 Lunch



14:00 - 16:00 SESSION 5 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS 
SYSTEMS

- Tissue Microarrayer Platform

Bring your own Tissue Blocks and make your TMA

- Digital Scanners  Image Analysis Software (Analyze your TMA Slide)

Bring your Tissue and TMA Slide and get them analysed

- Visit Exhibition Boots and look at instruments and tools which can enhance 
your work
Tissue Vacuum technology, Digital Scanners, Visual Imaging

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[Histonet] Histonet server issues

[Linda Margraf]

Hi, I just saw the messages about there being problems with the Histonet 
website http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The university was indeed doing some work on the server at the beginning of the 
month and they thought it wouldn't effect users much.   I checked and the 
website seems to be functional now and as far as I can tell, messages are going 
through as usual.   If you have persistent problems, please let me know at the 
histonet-ow...@lists.utsouthwestern.edumailto:histonet-ow...@lists.utsouthwestern.edu
 address.  Thanks.

Marvin Hanna's site that keeps the archive 
http://www.histosearch.com/histonet.html   also seems to be functioning 
properly.

Best regards,
Linda M
Histonet administrator

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Re: [Histonet] Plasma/thrombin clot for cell block

[Linda Prasad (SCHN)]

Hi Cheryl

This is the technique we use.

Thromboplastin Cell Block method

Principle:

The Thromboplastin cell block is very gentle on cells and allows a wide range 
of routine immunohistochemical stains to be done.
Plasma, treated with EDTA, is mixed with the specimen in the presence of 
thromboplastin and calcium ions to form a cell clot.
This method is not suitable for cells fixed in formalin or specimens of bile 
fluid (bile enzymes prevent clot formation)

Solutions:

1.  Normal Plasma, containing EDTA , use out of date plasma obtained from 
Blood Bank. Freeze in 2ml aliquots. This plasma has been tested for known 
viruses and is considered safe.

2.  Thromboplastin, use out of date thromboplastin obtained from 
Haematology.

3.  1% calcium chloride

4.  10% formalin

Method:

1.  Spin fluid using a centrifuge.
2.  Decant off supernatant
3.  Resuspend pellet in 3 drops of plasma.
4.  Add 3 drops of thromboplastin, mix
5.  Add 3 drops of Calcium Chloride, mix gently
6.  Allow to stand undisturbed for 15-20 minutes
7.  Add 5-8ml 10% formalin and allow to fix
8.  Place in a labelled tissue cassette and process as usual.

Reference: 
Furtado (1970) Stain Technol 45(1):19-23.

Thank you :)

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au


Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

♲  Please consider the environment before printing this email.

-Original Message-
From: Cheryl [mailto:tkngfl...@yahoo.com] 
Sent: Wednesday, 6 May 2015 2:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plasma/thrombin clot for cell block

HI Guys-

I need procedures including products to create a plasma cell block for non-gyn 
cytology.

The one we have uses bovine thrombin and calcium chloride (dihydrate) but it's 
gotten corrupted and I can't find the original resources.

Help?

Cheryl
tkngfl...@yahoo.com

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RE: [Histonet] Re: IHC and Oven Temperatures

[Sebree Linda A]

That's the reference I have always gone by since I first started doing IHC back 
in 19..

Linda A. Sebree

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
richardb...@charter.net [richardb...@charter.net]
Sent: Sunday, April 26, 2015 10:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: IHC and Oven Temperatures

The specific pages in the Dako Education Guide: Immunohistochemistry
Staining Methods, Fifth Edition are: Discussion on page 32 and
references on page 33. It's in the Fixation and Processing Chapter and
says no part of the process should have temperatures above 60C.

Rick Boen, BS, HTL (ASCP)
Histology Lab
St. Luke's Hospital
Duluth, Mn

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RE: [Histonet] IHC/SS QA/QC Sheets:

[Sebree Linda A]

Hi Craig,

As technologists, we of course QC all slides before they get to the 
pathologist(s) but that is not documented unless we put a repeat order in the 
case.  Then we add a note saying why a test needs repeating, i.e. positive 
control did not stain, patient tissue loss, etc.  But this is all done 
electronically, so no paper trail.  For general QC, we have gone to having a 
statement automatically populate to any case reports that include IHC/ISH 
staining but I'm assuming this could also work for HEs, Special Stains, etc.; 
I'm only familiar with the IHC/ISH portion of QCing.  Our statement says 
something along the lines of the controls staining appropriately and as 
expected.  The pathologist that signs out the case is attesting to that 
statement.  This statement has eliminated HUGE amounts of paper and filing.

Hope this helps,

Linda


Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Tuesday, April 21, 2015 3:16 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC/SS QA/QC Sheets:

Can someone help guide me on the right direction regarding how to organize 
daily QC sheets. Currently we have one per case (this is time consuming and I'm 
on overload of papers). Does anyone have a good solution and are you willing to 
share?

Thank you for your help,

Craig

Sent from my iPhone
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[Histonet] RE: Nuclear Artifact

[Blazek, Linda]

The first place I would look is to what may be happening before they reach me.  
If it's only one site with an issue, it sounds more like an issue at collection.
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa
Sent: Tuesday, April 21, 2015 1:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Nuclear Artifact

Hi HistoNetters:
I have run into quite a problem.  My lab currently processes all tissue types 
from 3 different sites.  Recently, we have been getting complaints from one of 
the sites that the biopsies have a nuclear artifact.  It is described as 
washed out or poor to no nuclear detail.  Pictures have been uploaded 
(Nuclear Artifact).  The Medical Director at said site is convinced that a 
processor error is occurring.  Our site is not seeing this on any of our 
slides.  Biopsies from all three sites are processed and embed together.  We 
have done all trouble shooting that we can think of.  Leica service has come to 
inspect our Peloris processor and all areas checked out as functioning 
properly.  The problem is not consistent daily.  Seems to be worst toward the 
end of the week.
We have been running the same processing protocol, staining protocol and 
cutting protocols for years now.  This problem has just developed over the last 
2 months.  Any ideas, no matter how far-fetched, would be greatly appreciated 
at this point.

Lisa Roy, HT(ASCP)
Histology Supervisor
LabCorp at St. Vincent Hospital
123 Summer St
Worcester, MA
(508)363-9420

-This e-mail and any attachments may contain CONFIDENTIAL information, 
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RE: [Histonet] Orange peel for GMS

[Linda Prasad (SCHN)]

What about plain boiled rice. Leave if out for a few days. You will get a lot 
of mould growing. Rice should be easy to cut as well.

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia
♲  Please consider the environment before printing this email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Saturday, 18 April 2015 2:55 AM
To: 'Blazek, Linda'; Bitting, Angela K.; 'T Williams'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

Hahahaha I bet not Linda!!!

Jessica

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, April 17, 2015 12:05 PM
To: Bitting, Angela K.; Piche, Jessica; 'T Williams'; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

Our blue cheese GMS would make you never want to eat it again!

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Friday, April 17, 2015 11:21 AM
To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

We just tried a moldy  strawberry. It was loaded with fungus!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Friday, April 17, 2015 7:11 AM
To: 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

I got a nice GMS control from some moldy chicken in my fridge. I am also trying 
the onion. I haven't stained the onion yet but it actually cut beautifully so I 
have high hopes. I let you know how it turns out. Have a nice weekend!

Jessica Piche, HT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of T Williams
Sent: Thursday, April 16, 2015 9:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Orange peel for GMS

I tried using a moldy orange peel as a GMS control, but the staining did not 
work.  I ran it in a 10 hour processing protocol.  Should I try something 
different?  Any suggestions would be extremely helpful - we're desperate for a 
GMS control.

Thanks,
T. Williams, HTL (ASCP), QIHC
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strictly prohibited. If you have received this information in error, please 
notify the sender immediately and delete these documents. Copyright (c) 
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IMPORTANT WARNING: The information in this message (and the documents attached 
to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
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please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.

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messages. If email is encrypted, the recipient will receive an e-mail 
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CONFIDENTIALITY NOTICE: This email and any attachments contain confidential 
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this information is prohibited from disclosing this information to any other 
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RE: [Histonet] Orange peel for GMS

[Blazek, Linda]

Our blue cheese GMS would make you never want to eat it again!

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Friday, April 17, 2015 11:21 AM
To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

We just tried a moldy  strawberry. It was loaded with fungus!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Friday, April 17, 2015 7:11 AM
To: 'T Williams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Orange peel for GMS

I got a nice GMS control from some moldy chicken in my fridge. I am also trying 
the onion. I haven't stained the onion yet but it actually cut beautifully so I 
have high hopes. I let you know how it turns out. Have a nice weekend!

Jessica Piche, HT(ASCP)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of T Williams
Sent: Thursday, April 16, 2015 9:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Orange peel for GMS

I tried using a moldy orange peel as a GMS control, but the staining did not 
work.  I ran it in a 10 hour processing protocol.  Should I try something 
different?  Any suggestions would be extremely helpful - we're desperate for a 
GMS control.

Thanks,
T. Williams, HTL (ASCP), QIHC
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the use of the individual or entity named above. The authorized recipient of 
this information is prohibited from disclosing this information to any other 
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intended recipient, you are hereby notified that any disclosure, copying, 
distribution or action taken in reliance on the contents of these documents is 
strictly prohibited. If you have received this information in error, please 
notify the sender immediately and delete these documents. Copyright (c) 
Waterbury Hospital


IMPORTANT WARNING: The information in this message (and the documents attached 
to it, if any) is confidential and may be legally privileged. It is intended 
solely for the addressee. Access to this message by anyone else is 
unauthorized. If you are not the intended recipient, any disclosure, copying, 
distribution or any action taken, or omitted to be taken, in reliance on it is 
prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
to it, if any), destroy any hard copies you may have created and notify me 
immediately by replying to this email. Thank you.

Geisinger Health System utilizes an encryption process to safeguard Protected 
Health Information and other confidential data contained in external e-mail 
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[Histonet] RE: ORO tissue falling off

[Linda Prasad (SCHN)]

Usually with the fatty tissues, I pick them up on superfrost slides and let it 
air dry  for 2-3 days at room temperature and then perform the ORO stains. So 
far they seem to stay on.

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

♲  Please consider the environment before printing this email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, 
Ms.
Sent: Thursday, 16 April 2015 1:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ORO tissue falling off

We are having difficulty with a particulate set of very, very fatty mouse 
livers.  The normal livers from this set stay on the slides the fatty livers 
fall off.  We have used different types of charged slides and we have even 
tried to drench the charged slides in Stay-On, dry them and then put the frozen 
tissues on (despirate times call for despirate measures).  No luck  Does anyone 
have any other ideas.  Help Help

Jo-Ann  Bader
Histology Coordinator
Goodman Cancer Research Center
1600 Pine Ave. W,
Room 312
Montreal Quebec, H3A 1A3
Email: jo-ann.ba...@mcgill.camailto:jo-ann.ba...@mcgill.ca
Office Tel:  514-398-5647
Lab:  Tel:  514-398-8270

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RE: [Histonet] Bouin's, formic acid and sirius red staining

[Linda Prasad (SCHN)]

Hi Tryrone
A Mass Trichrome stain will stain up the collagen in the diseased kidney. If 
the tissue has already been fixed in Bouin's Solution you can omit the picric 
acid step. Another good stain is the Curtis's Van Gieson Stain. Ive sent you 
the protocol for botht he stains. Hope it helps.   

Masson Trichrome

Principal
This method depends on the special action of phosphomolybdic acid when combined 
with red aniline dye. The acid lifts the colour first from the collagen and 
only later from the cytoplasm. The differentiation is stopped at the point 
where the Collagen only is colourless, and then another aniline dye (light 
green) is added to stain the collagen

Reagents:   

1.  Ponceau Acid Fuchsin 
Warning: Suspected Carcinogen – see MSDS
Acid fuchsin (Sigma Aldrich CI 42685) 0.1g
Ponceau de xylidene (CI 16150)  1g  
Distilled Water 99ml
When dissolved, add 1ml Glacial Acetic Acid

2.  Light Green
Warning: Suspected Carcinogen – see MSDS
Light green (CI 42095)  2g  
Glacial Acetic acid   2ml
Distilled water 98ml

3.  Weigert's Iron Haematoxylin or Celestine Blue/Haematoxylin

4.  1% Phosphomolybdic Acid
Phosphomolybdic acid5g
Distilled water 500ml
 

Procedure:  

1.  Bring sections to distilled water.  
2.  Celestine blue  10 minute.
3.  Wash well in water.
4.  Harris' haematoxylin10 minutes.
5.  Differentiate and blue  
6.  Ponceau acid fuchsin solution   10min
7.  Quick rinse in tap water
8.  1% Phosphomolybdic acid 5min
9.  Do not rinse in water
10. Light green solution2min 
11. Rinse excess stain rapidly in water.
12. Dehydrate, clear  mount

Results:  
Collagen, mucin green
Muscle, Fibrin  red
Nuclei  purple

Curtis's Van Gieson Stain

Principle:
This solution is based on the same formula as Van Gieson with the exception of 
the use of Ponceau S rather than acid fuschin. Glacial Acetic Acid is used 
rather than hydrochloric acid to sharpen the staining results.

Reagents: 

1.  1% aqueous Ponceau S 
Ponceau S (CI 27195)0.5g
Distilled water 50ml

2.  Curtis’s Van Gieson
1% aqueous Ponceau S10ml
Saturated aqueous picric acid   90ml
Glacial Acetic Acid 1.5ml

3.   Celestine Blue – Haematoxylin procedure

Procedure:
1.  Dewax and hydrate sections.
2.  Celestine blue 5 minute wash.
3.  Wash well in water.
4.  Harris' haematoxylin 5 minutes.
5.  Wash in water and blue (do not differentiate).
6.  Curtis Van Gieson stain 10 minutes (do not wash).
7.  Rinse in absolute alcohol 
8.  Dehydrate, clear and mount.

Results: 
CollagenRed
Muscle, other tissues   Yellow
Nuclei  Blue


Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

♲  Please consider the environment before printing this email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade
Sent: Thursday, 16 April 2015 7:10 AM
To: histonet
Subject: [Histonet] Bouin's, formic acid  and sirius red staining

Hello,

Some questions regarding Bouin's solution.

I was told, back when I was doing my PhD and new very little, that I should fix 
my fish in Bouin's as it will decalsify the bones. Well, Bouin's fixed fish 
were easier to cut than PFA fixed fish... but I read today that by adding 
formic acid the decalsification is better. (I must confess, that after Buoin's 
fixation I still had to soak the tissue face in some dilute nitric acid now and 
then...) Another reference said that the formaldehyde should be replaced with 
formic acid. So which is it: add formic acid or replace formaldehyde? And if 
the former, how much do you add?

Second question: a colleague and I want to stain for collagen in diseased 
kidneys. The fixative of choice for soft tissue is Bouin's... But the staining 
protocol called for a solution of picric acid and sirius red. Is the picric 
acid needed if I haven't washed the picric acid from the Bouin's fixation out 
of the tissue? I was told once that the picric acid was for contrast

RE: [Histonet] RE: ORO tissue falling off

[Linda Prasad (SCHN)]

I Know fatty tissue is such a pain to cut. Bryan Llewellyn gave some really 
good techniques. Im going to try them out myself :)

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au


Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

♲  Please consider the environment before printing this email.


-Original Message-
From: Caroline Miller [mailto:mi...@3scan.com] 
Sent: Thursday, 16 April 2015 10:44 AM
To: Linda Prasad (SCHN)
Cc: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: ORO tissue falling off

+1 to Linda, but I have found no difference on overnight vs multiple days.

Fatty liver is hard to do on all counts! It is tough enough sometimes to get a 
decent section on the slide.

Thanks for the other suggestions, certainly something I would try in the future

Yours
Caroline

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

 On Apr 15, 2015, at 5:07 PM, Linda Prasad (SCHN) 
 linda.pra...@health.nsw.gov.au wrote:
 
 Usually with the fatty tissues, I pick them up on superfrost slides and let 
 it air dry  for 2-3 days at room temperature and then perform the ORO stains. 
 So far they seem to stay on.
 
 Linda Prasad | Senior Scientist | Histopathology
 t: (02) 9845 3306 | f: (02) 9845 3318 | e: 
 linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au
 
 Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia 
 Locked Bag 4001, Westmead 2145, NSW Australia
 
 ♲  Please consider the environment before printing this email.
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, 
 Ms.
 Sent: Thursday, 16 April 2015 1:40 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] ORO tissue falling off
 
 We are having difficulty with a particulate set of very, very fatty 
 mouse livers.  The normal livers from this set stay on the slides the 
 fatty livers fall off.  We have used different types of charged slides 
 and we have even tried to drench the charged slides in Stay-On, dry 
 them and then put the frozen tissues on (despirate times call for 
 despirate measures).  No luck  Does anyone have any other ideas.  Help 
 Help
 
 Jo-Ann  Bader
 Histology Coordinator
 Goodman Cancer Research Center
 1600 Pine Ave. W,
 Room 312
 Montreal Quebec, H3A 1A3
 Email: jo-ann.ba...@mcgill.camailto:jo-ann.ba...@mcgill.ca
 Office Tel:  514-398-5647
 Lab:  Tel:  514-398-8270
 
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 whom they are addressed. If you are not the intended recipient, please delete 
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RE: [Histonet] C3d?

[Sebree Linda A]

Cleveland Clinic does it by IHC and I think also by IF.

Linda A. Sebree 
University of Wisconsin Hospital  Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Wednesday, April 01, 2015 6:14 PM
To: HistoNet
Subject: [Histonet] C3d?

Hello Again My Dear Netters,

One of our pathologists was wondering if anyone out in Histoland is performing 
a C3d stain?

If so what type: IHC, IF?

Thanks oodles!

Paula  :-)
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RE: [Histonet] Allowable temperature range

[Linda Prasad (SCHN)]

If it's in formalin it can just stay at room temperature.

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au


Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia
♲  Please consider the environment before printing this email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim H
Sent: Thursday, 2 April 2015 1:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Allowable temperature range


What is the allowable temperature range for a histology specimen after 
collection in formalin for shipping and storage?
 
Any ideas?
 Tim

  
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RE: [Histonet] Tol Blue

[Linda Prasad (SCHN)]

The mast cells are meant to turn purple. It’s a metachromatic stain. So the tol 
blue stains the tissue blue and the mast cells purple.

Mast cell granules  Purple
Acid Mucosubstance  Purple
Nuclei  Blue

Mast cells are rich in heparin. This allows them to be stained via acid 
mucopolysaccharide and metachromatic staining techniques. Churukian and Schenk 
developed this metachromatic dye-technique to reduce the excessive background 
staining that is common with metachromatic dye techniques.


Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au
Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia
♲  Please consider the environment before printing this email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn
Sent: Wednesday, 25 March 2015 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tol Blue

Try the method at:

http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm

Bryan Llewellyn

Kimberly Marshall wrote:
 ?Hello my fellow Histo Techs.  Have a question I just know someone out there 
 can answer for me.

 In canine tissue, we are having problems with the Tol Blue for mast cell.  Am 
 experiencing metachromasia, or the mast cells turning purple not blue.  I 
 have attempted to decrease time, or add time, but its not helping.  My 
 pathologist says he has had this issue before.  So question is.  Could it be 
 the mast cell in a dog does not stain the same? Is there another stain that 
 may work?  Any help will be much appreciated.


 Thanks in Advance.

 Kimberly Marshall H.T.(ASCP)






 Kimberly Marshall H.T.(ASCP)

 Histology/Lab Supervisor

 Toll Free 1-800-426-2099

 Fax 801-584-5104

 PO Box 17580

 Salt Lake City, Utah 84107

 www.animalreferencepathology.comhttp://www.animalreferencepathology.com/



 Advancing the art and science of veterinary medicine



 [cid:image001.jpg@01CF8F87.A0BD4830]

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RE: [Histonet] BS in Histotechnology

[Blazek, Linda]

Fortunately times have changed.  The person I replaced in the late 70's early 
80's had been brought in from the hospital laundry and was trained.  They 
were still pouring embedding molds then.  She did a beautiful job at cutting 
and staining HE slides and 2 or 3 specials but that was all there was to do.  
This world has come a lot farther than those days of the 80's and 70's.  We 
have grown into fully capable labs that don't have to send work out to the big 
reference centers to have tests done.  I remember a class with Lee Luna when he 
said We have to excel and learn these immuno procedures or the MT's were going 
to take them away from us.  We learned and they didn't!  We may not be 
recognized at the level with MT's but we are slowly changing and getting there. 
 At least now an associates is required and CEU's are required.  I think that's 
progress.  If you're looked down on in your present position move on!  It's not 
worth it.
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A
Sent: Tuesday, March 24, 2015 1:53 PM
To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: RE: [Histonet] BS in Histotechnology

That was nicer than the pathologist who told me years ago, any monkey could be 
trained to do my job.  I basically did not take the job I was interviewing for 
at the time.  At least the next interview went a lot better and I did take the 
job.

Pam

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 24, 2015 12:30 PM
To: Sue; Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: RE: [Histonet] BS in Histotechnology

I agree, BUTas long as many pathologists think you can teach any 
trained dog how to section histology will never have the recognition those of 
us that have studied and trained deserve.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, March 24, 2015 12:59 PM
To: Timothy Morken
Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald
Subject: Re: [Histonet] BS in Histotechnology

This is a fight that we continue to have with hospital administration.  In my 
opinion histologists are just as important and needed as MT.  Even though there 
is an increase in automation in pathology the hands on of a histologists is 
most important.  The fact that hospital still consider a lower entry job is the 
reason there are not more of us.  It is quite frustrating. 
  
Sue 
TJUH 
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[Histonet] job opening

[Blazek, Linda]

I'm posting this for Matt .  And it is a great place to work!
Linda


Hey All
We have a full time position now open at the Dayton Children's Hospital. This 
is a small lab, we process between 15 and 50 blocks per day. Pay will be 
determined according to experience. Must be certified or certified eligible 
with-in two years. Great place to work, little stress, great people and 
benefits. Follow the link to apply.

http://www.childrensdayton.org/cms/sitelet/job_postings/index.html

Click on Technical and Full Time


Matt Chase, HT (ASCP)
Supervisor of Pathology

Dayton Children's
One Children's Plaza
Dayton, Ohio 45404-1815
Office Number: 937-641-3000 Ext 8229
Fax Number: 937-641-5482
Email: cha...@childrensdayton.orgmailto:cha...@childrensdayton.org
Website: childrensdayton.org

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[Histonet] FW: Masson's trichrome stain

[Linda Margraf]

Here is a message from Justine...

 

From: Justine Lanzon [mailto:justinelan...@hotmail.com] 
Sent: Thursday, March 05, 2015 5:36 AM
To: lindamarg...@gmail.com
Subject: Masson's trichrome stain

 

Hi,

I am doing a write up on Masson's trichrome stain however I cannot answer
these two questions:

- Why are plastic forceps used instead of metal ones to hold the stained
slide?

- Why do we not rinse before Alinine blue?

 

Can you please help me?

 

Many Thanks,

Justine Lanzon

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[Histonet] RE: Mushrooms for GMS fungus control

[Linda Prasad (SCHN)]

I used strawberries for a fungal control. Worked really good.

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

♲  Please consider the environment before printing this email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Saturday, 7 March 2015 4:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mushrooms for GMS fungus control

How about mushrooms?  Has anyone had any success using mushrooms as a GMS 
fungus control?

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA


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[Histonet] RE: need light for flotation bath

[Blazek, Linda]

I found them at Lowe's.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Preiszner, 
Johanna
Sent: Monday, March 02, 2015 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] need light for flotation bath

Hi,

we have a Fisher Tissue Prep 135 flotation bath and the light died. The 
instrument is no longer manufactured and I cant find a source for the 
fluorescent light tube. There are multiple baths for sale on ebay but nobody 
sells the light alone.

Could someone help me out? Would something from Home Depo do?


Thanks,
Hanna




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[Histonet] Kawamoto Method

[Prentice, Linda]

I am interested in using the Kawamoto tape method but we have not found a 
source for the supplies. Can anyone direct me to one?
Thanks in advance

Linda Prentice
UCSF Preventive and Restorative Dental Sciences
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RE: [Histonet] Receipt of specimens

[Blazek, Linda]

I actually use three different times for tracking since it means different 
things to different people.  I track collection to sign out, received in the 
lab to sign out, and report ready to sign out.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik
Sent: Tuesday, February 10, 2015 2:25 PM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Receipt of specimens

Tanya, you can probably put either date/time into the receive date/time prompt 
of your LIS.  It just depends on how you want to use that information.  For 
instance, perhaps you want to look at turn around times.
From the lab's perspective, the 'clock' starts when the sample is 
received
in the lab.  On the other  hand, from the physician's perspective, it starts at 
sample collection and from the organization's perspective the clock might start 
when the sample was put in the fridge.

If it was 'me', I'd probably put the date/time the sample was received in the 
lab and I'd make sure the other information was either scanned into the
case via paper or somehow noted somewhere else.   The reason for my thinking
is that those cases received off shift are going to skew your turnaround times 
if that's something you measure.

Bottom line, it just depends on what you're using that information for.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Monday, February 09, 2015 9:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Receipt of specimens

I am curious as to how everyone handles the receipt of their surgical, and 
Cytology specimens in to the lab? Especially on the off shift hours? Other 
clinical lab specimens are received directly in the computer in Specimen 
Processing when they come in the door from remote locations or via the tube 
system, then delivered to departments. During the day, specimens get dropped 
off directly to Pathology by the various departments collecting (ie. OR, 
Radiology, etc). On our off shifts, specimens get received by Specimen 
Processing(date/time stamped and initialed)  and put in the fridge for 
Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, 
for the receive date, they go by the date the specimens are received (or 
unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen 
Processing received them in to the actually laboratory where the specimens are 
then controlled.
Any input is appreciated! Thanks!
Tanya

Tanya G. Abbott
Manager Technologist
Histology/Cytology
St Joseph Medical Center
(phone) 610-378-2635

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[Histonet] HNF-1 beta

[Sebree Linda A]

Good morning,

We have a pathologist that has a case he wants sent out for HMF-1β (hepatocyte 
necrosing factor-1β) .  Are there any reference labs out there that offer this?

Thanks,
Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

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[Histonet] RE: Cassette Labeler

[Blazek, Linda]

I second that!  I love mine.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Tuesday, January 27, 2015 12:25 PM
To: Debbie Granato; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cassette Labeler

Hello, we recently purchased the Primera unit from Creative waste solutions.
http://www.cwsincorp.com/

we like it. I would be happy to discuss it further if you like.


Helen L. Fedor 

Oncology Tissue Services, Manager
Johns Hopkins University
411 N. Caroline St
Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231

410-614-1660

http://tmalab.jhmi.edu/






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Granato
Sent: Monday, January 26, 2015 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette Labeler

Our lab is currently looking into purchasing a cassette and slide labeler.

We are a small lab and are looking for a stand- alone unit with the flexibility 
to be used with the computer or bar code scanner at a later date.

I would appreciate any feedback or suggestions for any model that may fulfill 
our needs.

Thank you,

Debbie Granato HT(ASCP)


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RE: [Histonet] RE: Cassette Labeler

[Blazek, Linda]

I've had mine longer than that and don't have a problem.  I have a feeling that 
you may need a bit of service on it.  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Auge
Sent: Tuesday, January 27, 2015 1:11 PM
To: Helen Fedor
Cc: histonet@lists.utsouthwestern.edu; Debbie Granato
Subject: Re: [Histonet] RE: Cassette Labeler

I also have a Primera slide printer. It worked well at first but now it is
2 years old and prints very slowly. I can hand write up slides faster than they 
print. I will be in the market for a new slide printer soon and will not be 
going back to Primera.

-- 

Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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