Re: [Histonet] Antibody diluent

[Lindrud, Scott via Histonet]

What is everyone's take on what needs to be done from a verification/validation 
standpoint if you switch diluents for your concentrated antibodies?  The CAP 
summary of recommendations on the validation IHC antibodies doesn't 
specifically mention the changing of diluent as one of the variables.

If I change the diluent on my ER and Her2 concentrates, do I need to do a 
complete re-validation or just a quick verification using a few positive and 
negative cases?  I'm not really liking the idea of a complete re-validation!

Thanks for any info!

Scott


Scott A. Lindrud, MLSCM(ASCP)CTCM  | Histopathology Technical 
Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201




-Original Message-
From: Jeanine Ronkowski 
Sent: Tuesday, March 23, 2021 2:21 PM
To: Paula Keene Pierce ; Maria Cruz 
; Morken, Timothy 
Cc: Histonet 
Subject: Re: [Histonet] Antibody diluent

All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s 
really no such thing as a universal diluent like the ones offered by Leica and 
Agilent.  We get ours from Biocare, they offer five different diluents with 
different pHs and chemical makeups and we use three of these. I checked with my 
Biocare rep and iit seems that the one that’s most similar to Leicas is called 
Monet Blue.  I suggest you contact your Bocare rep and see if they can send you 
a sample of one or more them. Good  luck,Jeanine


Sent from Yahoo Mail for iPhone


On Tuesday, March 23, 2021, 2:43 PM, Paula Keene Pierce via Histonet 
 wrote:

I just purchased antibody diluent from Electron Microscopy Sciences.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb

On Tuesday, March 23, 2021, 01:36:12 PM CDT, Morken, Timothy via Histonet 
 wrote:

 Maria, we went back to using Dako Diluent, which we had just switched away 
from to save money!

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Maria Cruz via Histonet 
Sent: Tuesday, March 23, 2021 10:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Antibody diluent

Like other folks in the histo community, I’ve recently experienced the 
inability to buy diluent from Leica.  Now wondering what others are doing to 
handle this problem.  TIA!
Maria
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Re: [Histonet] Question about accessioning outside consult cases

[Lindrud, Scott via Histonet]

Hi Martha,

We have been using Beaker for over 2 years now.  We created another accession 
that identifies cases that are being sent to us as a consult/referral (both 
Cyto and Histo).   It works pretty well for us.

Scott


Scott A. Lindrud, MLS(ASCP)CT  | Histopathology Technical 
Specalist/Cytotechnologist

P: 320-231-4520
F: 320-231-4503
scott.lind...@carrishealth.com

Carris-Health
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201






-Original Message-
From: Martha Ward-Pathology 
Sent: Thursday, December 10, 2020 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about accessioning outside consult cases

We are in the process of switching to AP Beaker and in the midst of the build.  
  My question involves how others are handling outside consult surgical and/or 
cytology cases.   Do you assign them an unique number that identifies them as a 
consult as opposed to an inside case - SO20- verses S20-? We 
currently assign them as just another surgical case but I know some places do 
have outside consult designations.

What is everyone doing?

Thanks in advance for your input.

Martha Ward, MT ASCP (QIHC)
Manager, Molecular Diagnostics Lab
Wake Forest Baptist Medical Center
Winston-Salem, NC 27157

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[Histonet] Weekend Coverage for Small Pathology Labs

[Lindrud, Scott via Histonet]

Hi Histonet,

I apologize if this is being re-posted but I did not see this message on the 
daily Histonet Digest so I'm not sure if it ever got posted.

I was wondering if anyone would be willing to share their experience with 
weekend Histology coverage for smaller Pathology labs?  When I say small, I'm 
saying about 30,000-33,000 blocks per year (avg 130-140/day) with automated IHC 
and special staining.  Our lab is in a rural area where all but one of the 
histotechs live between 25-50 miles away.  Winter weather has played a role in 
numerous instances of trying to get a histotech to the lab on a bad weather 
Saturday.

Our lab currently has a histotech come in on Saturday morning to set up a gross 
for about 16-20 cases which will be put on processing run to come off on Monday 
morning.  The majority of the cases are from colonoscopies, colposcopies, skin 
excisions, gallbladder/appendix, and placentas.

I'm asking this to see if there is a necessity for the Saturday coverage for a 
lab our size.  If the industry standard is that most labs of smaller size have 
some sort of Saturday histology coverage, then we're good to go.  But if most 
smaller labs aren't providing some sort of weekend coverage, then I feel more 
confident talking to management for our histotechs about the necessity of 
providing the Saturday coverage.   I just don't know what other smaller labs 
are doing.

Thanks for any opinions or information!

Scott A. Lindrud, MLSCM(ASCP)CTCM | Histopathology Technical Specialist
Phone: 320-231-4406
Fax: 320-231-4305
scott.lind...@carrishealth.com



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[Histonet] Fat Aspirates for Amyloidosis

[Lindrud, Scott via Histonet]

Hi Histonetters,

Would anyone be willing to share how they process a Fat Pad aspirate for 
Amyloidosis?  Our method does not provide a very good result and we're looking 
to improve on how we do this.

We collect direct aspirates using an 18 gauge needle and make air dried smears 
and stain with Congo Red.  We also get additional passes and place directly in 
10% NBF and will try to make a cell block using histogel out of them.  Problem 
is fat floats and it is difficult to get a good cell block made.

I would think spinning the 10%NBF and filtering out the fat and placing 
directly into a biopsy bag or wrapping in paper would be the way to go.  After 
you process the block, I'm guessing all the lipid will process out and there 
would only be cell membranes from the fibroadipose tissue left over.  Wouldn't 
you need a significant amount of fat to process to have anything to embed?  
I've seen suggestions on other websites suggesting 35-50 mg of fat.  Maybe we 
need to go to a larger needle, maybe 16 gauge?

Any ideas would be greatly appreciated!

Scott



Scott A. Lindrud, MLS(ASCP) CM CT CM
Histopathology Technical Specialist/Cytotechnologist
CarrisHealth Rice Memorial Hospital
301 Becker Ave SW | Willmar | MN | 56201
scott.lind...@carrishealth.com
320-231-4406/4520
320-231-4503 (fax)

Carris Health is a new entity created to deliver health care to West Central 
and Southwest Minnesota. Carris Health is a partnership between CentraCare 
Health, Rice Memorial Hospital and ACMC Health. 
www.carrishealth.com

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[Histonet] MMR Negative controls

[Lindrud, Scott via Histonet]

Good Morning HistoNet,

Would anyone be willing to share what they use for control tissue for MMR 
testing?  Finding negative expressing tumor is becoming more difficult for us 
to find and we're wondering if there is an alternate negative tissue that would 
be acceptable.

NordiQC recommends using Tonsil as a positive tissue control and Colon 
Adenocarcinoma that has a loss of expression as a negative tissue control for 
all four antibodies (MLH1, MSH2, MSH6, PMS2).  Stromal cells in the negative 
tissue control should have positive nuclear staining and serve as a positive 
internal control.  NordiQC doesn't have any recommendations for alternative 
tissue to use as a negative control.

Anyone doing anything differently?

Thanks,

Scott

Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201
WP(320)231-4406
Fax(320)231-4861
scott.lind...@rice.willmar.mn.us




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[Histonet] Negative IHC Control for Her2

[Lindrud, Scott via Histonet]

Hi All,

We are having an internal debate regarding Her2 IHC control tissue in our lab.  
We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 
staining tissue taken from lumpectomy/resection cases.  I'm in the process of 
searching for more tissue to use in future control blocks and it can be 
difficult to find tissue that is 0+ and 3+.

I've discussed this with our pathologist in charge of Histology and he says 
that we don't need to run all 4 of the cores as controls.  He says all we need 
to run is a positive control and a negative control.  He says the positive 
control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 
1+.

I respectfully disagreed with him and said the negative control is not meant 
for accessing staining interpretation but to verify the sensitivity and 
specificity of the actual antibody-antigen reaction.  I said a 1+ is still 
staining the tissue where there is no staining in a 0+ reaction.  The 1+ is not 
a negative staining reaction, but a negative interpretation.   The pathologist 
says I'm wrong.

The CAP in its checklist says "It is also important to assess the specificity 
of each antibody by a negative tissue control, which must show no staining of 
tissues known to lack the antigen".To me, a 1+ Her2 staining reaction shows 
that that tissue has antigen and should not be used as a negative control.

So, after saying all that, can/should  1+ Her2 breast tissue be used as a 
negative tissue control?  Seems pretty straight forward to me, but I'm just a 
Cytotechnologist/Histotech.

Thanks for any input!

Scott

Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201
WP(320)231-4406
Fax(320)231-4861
scott.lind...@rice.willmar.mn.us




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Re: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer

[Lindrud, Scott via Histonet]

Miranda,

We use the Dako Artisan for special staining and it performs great.  We've been 
utilizing ours for about 7 years now.  We use the following staining protocols:

Iron
PAS
PAS/D
GMS
Retic
Trichrome
AFB
Warthin Starry

The staining is consistent and reproducible for all of our protocols.  The 
stainer is easy to set up and maintain.  Ours has been super reliable and when 
we have had problems, their technical support is helpful. Getting a Field 
Service Engineer out to make repairs for the few times we've needed it has 
always been prompt (within a day).

I have no experience with the Benchmark so I can't compare that to the Artisan, 
but I would have to give the Artisan Link a "2 thumbs up" review!

Scott Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist
Rice Memorial Hospital
Willmar, MN



-Original Message-
From: Miranda Giorgi [mailto:mgio...@incdx.com]
Sent: Monday, July 03, 2017 1:38 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer

Hello,

Our lab is currently evaluating the Benchmark Special Stainer from Ventana and 
we are finding the stain quality and consistency to be lacking, especially the 
retic and trichrome.  While we have the opportunity, we would like to consider 
an alternative platform, the Dako Artisan.  Has anyone used one or both of 
these plans?  If so, can you provide any feedback on your experience, including 
any pros or cons?

Any feedback is very much appreciated as we do not want to be stuck with an 
instrument that is not going to work.

Thanks in advance!

Miranda Giorgi, HTL (ASCP)cm
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[Histonet] Decal times for Bone Marrow Biopsies

[Lindrud, Scott via Histonet]

Happy Friday to Everyone,

I have a question about decal for Trephine collected bone marrows.  We 
currently decal our Trephine collected bone marrow biopsies for 1 hour in 
Cal-Rite by Richard Allan, a mixture of formic acid and formaldehyde.  Although 
this seems to work adequately most of the time, there are times when the decal 
isn't as thorough as we would like.

My questions for everyone are (if you are willing to share):


1.   What do you decal your bone marrows with and for how long?



2.   Are you able to perform Fluorescence In Situ Hybridization (FISH) or 
Chromogenic In Situ Hybridization (CISH) testing on your biopsies with your 
decal protocol?


Thanks for any information you are willing to share!

Scott Lindrud

Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist
Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201
WP(320)231-4406
Fax(320)231-4861
scott.lind...@rice.willmar.mn.us




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